Natrinema gari sp. nov., a halophilic archaeon isolated from fish sauce in Thailand Page 1 of 2 Natrinema gari sp. nov., a halophilic archaeon isolated from fish sauce in Thailand 1234 Wanaporn Tapingkae , Somboon Tanasupawat , Takashi Itoh , Kirk L. Parkin , 155 Soottawat Benjakul , Wonnop Visessanguan and Ruud Valyasevi - Author Affiliations 1Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand 2Department of Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand 3Japan Collection of Microorganisms, RIKEN BioResource Center, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan 4Department of Food Science, University of Wisconsin, Madison, WI 53706, USA 5National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Paholyothin Rd, Klong 1, Klong Luang, Pathumthani 12120, Thailand Correspondence Wonnop Visessanguan
[email protected] Abstract Two Gram-negative, rod-shaped, halophilic archaea, designated strains HIS40-3T and HDS3-1, were isolated from anchovy fish sauce (nam-pla) collected from two different locations in Thailand. The two strains were able to grow at 20–60 °C (optimum 37–40 °C), at 1.7–5.1 M NaCl (optimum 2.6–3.4 M NaCl) and at pH 5.5– 8.5 (optimum pH 6.0–6.5). Hypotonic treatment with less than 1.7 M NaCl caused cell lysis. The major polar lipids of the isolates were C20C20 and C20C25 derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate, two glycolipids and one unidentified lipid. The DNA G+C contents were 64.0–65.4 mol%. In addition to phenotypic and chemotaxonomic characteristics, phylogenetic analysis based on 16S rRNA gene sequence similarities showed that strains HIS40-3T and HDS3-1 were related most closely to species of the genus Natrinema.