PGAM2 Polyclonal Antibody

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For Research Use Only PGAM2 Polyclonal antibody Catalog Number:25518-1-AP www.ptgcn.com Catalog Number: GenBank Accession Number: Recommended Dilutions: Basic Information 25518-1-AP NM_000290 WB 1:500-1:1000 Size: GeneID (NCBI): 500 μg/ml 5224 Source: Full Name: Rabbit phosphoglycerate mutase 2 (muscle) Isotype: Calculated MW: IgG 28 kDa Purification Method: Observed MW: Antigen affinity purification 29 kDa Immunogen Catalog Number: Applications Tested Applications: Positive Controls: WB,ELISA WB : mouse testis tissue; Species Specificity: human, mouse Phosphoglycerate mutase (PGAM), an important enzyme in the glycolytic pathway, catalyzes the transfer of a Background Information phosphate group between the 2 and the 3 positions of glyceric acid. The muscle-specific isoform (type M, PGAM2) of phosphoglycerate mutase (PGAM) is a housekeeping enzyme and it catalyzes the conversion of 3-phosphoglycerate into 2-phosphoglycerate in the glycolysis process to release energy. It is encoded by the Pgam2 gene. In addition, it is demonstrated that PGAM2 locates both in cytoplasm and nuclei, and takes part in the glycometabolism process of cytoplasm and nuclei(PMID: 18499067). Defects in PGAM2 are the cause of glycogen storage disease type 10 (GSD10). This antibody is specific for PGAM2. Storage: Storage Store at -20ºC. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech T: 4006900926 E: [email protected] W: ptgcn.com Group brand and is not available to purchase from any other manufacturer. Selected Validation Data mouse testis tissue were subjected to SDS PAGE followed by western blot with 25518-1-AP (PGAM2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours..

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Oxidative Stress Activates SIRT2 to Deacetylate and Stimulate Phosphoglycerate Mutase
Published OnlineFirst May 1, 2014; DOI: 10.1158/0008-5472.CAN-13-3615 Cancer Tumor and Stem Cell Biology Research Oxidative Stress Activates SIRT2 to Deacetylate and Stimulate Phosphoglycerate Mutase Yanping Xu2,3, Fulong Li2,3, Lei Lv1,2, Tingting Li2,3, Xin Zhou2,3, Chu-Xia Deng4, Kun-Liang Guan1,2,5, Qun-Ying Lei1,2, and Yue Xiong1,2,6 Abstract Glycolytic enzyme phosphoglycerate mutase (PGAM) plays an important role in coordinating energy production with generation of reducing power and the biosynthesis of nucleotide precursors and amino acids. Inhibition of PGAM by small RNAi or small molecule attenuates cell proliferation and tumor growth. PGAM activity is commonly upregulated in tumor cells, but how PGAM activity is regulated in vivo remains poorly understood. Here we report that PGAM is acetylated at lysine 100 (K100), an active site residue that is invariably conserved from bacteria, to yeast, plant, and mammals. K100 acetylation is detected in ﬂy, mouse, and human cells and in multiple tissues and decreases PGAM2 activity. The cytosolic protein deacetylase sirtuin 2 (SIRT2) deacetylates and activates PGAM2. Increased levels of reactive oxygen species stimulate PGAM2 deacetylation and activity by promoting its interaction with SIRT2. Substitution of endogenous PGAM2 with an acetylation mimetic mutant K100Q reduces cellular NADPH production and inhibits cell proliferation and tumor growth. These results reveal a mechanism of PGAM2 regulation and NADPH homeostasis in response to oxidative stress that impacts cell proliferation and tumor growth. Cancer Res; 74(13); 1–13. Ó2014 AACR. Introduction contains two PGAM genes, PGAM1 (also known as PGAM-B), Enhanced glycolysis, commonly referred to as the "Warburg which is expressed in brain and most other tissues, and PGAM2 effect" (1), is a distinctive and prominent feature of cancer cells.
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