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Responsive Transporter Genes Within the Murine Intestinal–Pancreatic Axis Form a Basis of Zinc Homeostasis

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Responsive Transporter Genes Within the Murine Intestinal–Pancreatic Axis Form a Basis of Zinc Homeostasis Responsive transporter genes within the murine intestinal–pancreatic axis form a basis of zinc homeostasis Juan P. Liuzzi*, Jeffrey A. Bobo*, Louis A. Lichten*, Don A. Samuelson†, and Robert J. Cousins*‡ *Nutritional Genomics Laboratory, Center for Nutritional Sciences, and †Small Animal Clinical Sciences Department, University of Florida, Gainesville, FL 32611 Contributed by Robert J. Cousins, August 23, 2004 Zn homeostasis in animals is a consequence of avid uptake and lothionein (MT) gene expression, which is usually concurrent retention, except during conditions of limited dietary availability, with enhanced cellular Zn acquisition, has been integrated into and͞or factors such as parasites, which compete for this micronu- all of these aspects of cellular Zn trafficking. trient or compromise retention by the host. Membrane proteins Zn transporters are essential components of systems that that facilitate Zn transport constitute the SLC30A (ZnT) and SLC39A influence Zn trafficking in times of dietary depletion or excess, (Zip) gene families. Because dietary recommendations are based such as during acute and chronic physiologic stress (e.g., infec- on the balance between intestinal absorption and endogenous tion and inflammation) and during pregnancy and lactation. losses, we have studied Zn transporter expression of the murine Therefore, experiments with mice were designed to show the intestinal–pancreatic axis to identify transporters that are likely to differential expression of ZnT and Zip transporter genes asso- be involved in homeostatic control of Zn metabolism. Marked ciated with dietary Zn restriction and excess. The data presented tissue specificity of expression was observed in Zn-depleted vs. here identify the transporters critical for regulation of Zn Zn-adequate mice. As shown by quantitative PCR, Western blot homeostasis in intestinal absorptive cells, as well as pancreatic analysis, and immunohistochemistry, intestinal Zip4 was markedly acinar cells. up-regulated in response to Zn-depletion conditions. The increased abundance of Zip4 is concentrated at the apical membrane of Materials and Methods enterocytes. There are 16 ZnT and Zip transporters expressed in Mice and Treatments. Young adult male CD-1 strain mice (Charles pancreas. Only two, ZnT1 and ZnT2 (both cellular Zn exporters), River Breeding Laboratories) were housed and fed as described show a progressive down-regulation under Zn-depleted condi- in detail in refs. 7 and 8. Mice were fed one of three dietary Zn tions. In Zn-adequate mice, ZnT1 is diffusely distributed in acinar levels [ZnϪ (Ͻ1mg͞kg), Zn normal (ZnN; 30 mg͞kg), or Znϩ ␣ cell cytoplasm and colocalizes with -amylase but is not detected (150 mg͞kg)], which represent depleted, adequate, or excessive in pancreatic islets. In acinar cells during Zn depletion, ZnT1 is intakes of Zn, respectively. Mice were fed individually for up to localized to the plasma membrane. Intestinal Zip4 up-regulation by 21 days. Blood was collected by cardiac puncture under halo- Zn-depletion conditions is dampened in metallothionein knockout thane anesthesia for measurement of the serum Zn concentra- mice, suggesting that intracellular Zn pools influence these re- tion (7), and preparation of peripheral blood mononuclear cells sponses. The results show that Zn transporter expression in the (PBMCs) was performed by using NycoPrep 1.077 (Life Tech- intestinal–pancreatic axis is a component of the homeostatic reg- nologies, Grand Island, NY) gradient centrifugation. MT knock- ulation of this micronutrient. out mice and appropriate 129͞SvCP strain controls (The Jackson Laboratory) were used as described (9) for some experiments. ur understanding of how Zn is metabolized in animals and Protocols were approved by the University of Florida Institu- Ohumans is advancing rapidly by examination of membrane tional Animal Care and Use Committee. proteins that facilitate Zn transport. Zn transporters fall into two gene families, SLC30A and SLC39A (1). These families are Real-Time PCR for Transporter Expression. Tissues (intestine, 7 cm; commonly referred to as ZnT and Zip transporters, respectively. starting 0.3 cm caudal to the pyloric sphincter and pancreas) Zn metabolism has been defined partially in studies with were excised rapidly after exsanguination and homogenized humans and animals by using radioactive and stable isotopes immediately in TriPure reagent (Roche Diagnostics), and total (reviewed in ref. 2). Kinetic analyses and metabolic modeling RNA was isolated. RNA from PBMCs was also obtained by using SCIENCES have established the major pathways by which this micronutrient TriPure reagent. Before quantitative PCR (Q-PCR), all samples is processed and how organ systems produce an effective ho- were treated with DNase (Ambion, Austin, TX). Sequences for APPLIED BIOLOGICAL meostatic control over absorption and excretion. The current transporter genes were obtained from GenBank, and primers Dietary Reference Intake (Institute of Medicine of the National and probe sets were designed by using PRIMER EXPRESS software Academies,Washington, DC) recommendations for Zn intake by (version 2.0, Applied Biosystems). These primers and probes are humans are based on a balance between intestinal absorption given in Table 1, which is published as supporting information on and endogenous losses by using these metabolic assessments (3). the PNAS web site. For Q-PCR, we used TaqMan chemistry for Dietary Zn restriction leads to an up-regulation of the medi- fluorescence detection and one-step reverse-transcriptase reac- ated component of the Zn-absorptive pathway in rodents, pre- tions (Applied Biosystems) and a fluorimetric sequence detector sumably by means of changes programmed in intestinal cells (4). (Bio-Rad). The 18S rRNA primer–probe set was obtained from Loss of endogenous Zn from intestinal and pancreatic secretions Applied Biosystems, and the set for murine MT-1 was developed are concomitantly reduced during Zn restriction (reviewed in refs. 5 and 6). Urinary losses of Zn are low, indicative of high reabsorptive capacity, and they are refractory to change over a Abbreviations: MT, metallothionein; Q-PCR, quantitative PCR; PBMC, peripheral blood wide Zn-intake range (6). Furthermore, metabolic studies have mononuclear cell; ZnN, Zn normal; AE, acrodermatitis enteropathica. identified organ systems that influence Zn metabolism and its ‡To whom correspondence should be addressed at: University of Florida, 201 FSHN, P.O. Box response to physiologic stimuli, including hormones, cytokines, 110370, Gainesville, FL 32611-0370. E-mail: cousins@ufl.edu. and growth factors (reviewed in ref. 5). Induction of metal- © 2004 by The National Academy of Sciences of the USA www.pnas.org͞cgi͞doi͞10.1073͞pnas.0406216101 PNAS ͉ October 5, 2004 ͉ vol. 101 ͉ no. 40 ͉ 14355–14360 Downloaded by guest on September 29, 2021 previously (7). Relative quantitation used five log10 standard curves with 18S rRNA as the normalizer, as described in refs. 8 and 10. Antibody Production. The peptides GTRPQVHSGXE (11), HAQKDSGSHP, and AEETPELLNPETRRL (12) were used to produce antibodies in rabbits to ZnT1, ZnT2, and Zip4, respectively. Peptides were analyzed by MS to assess purity. A C-terminal cysteine was added to each peptide to facilitate conjugation to keyhole limpet hemocyanin (KLH) to create the antigens and for conjugation to Sulfo-link (Pierce) matrix to immobilize each peptide for affinity chromatography. These methods are described in refs. 11 and 13. Goat anti-␣-amylase IgG was obtained from Santa Cruz Biotechnology. Western Blot Analysis of Membrane Proteins. Intestinal samples (as described above) were homogenized immediately in cold buffer (20 mM Hepes, pH 7.4͞1 mM EDTA͞300 mM mannitol) containing a protease inhibitor mixture (Sigma) by using a Potter–Elvehjem homogenizer. A membrane protein prepara- tion (pellet) was prepared by centrifugation (100,000 ϫ g) after nuclei and debris were first removed by centrifugation at 1,000 ϫ g. The proteins were resolved by PAGE using 10% gels, trans- ferred to poly(vinylidene difluoride) membrane (Millipore), incubated with the appropriate affinity-purified IgG, and visu- alized by chemiluminescence detection with a PhosphorImager (Molecular Dynamics). Details of these procedures have been presented (11, 13). Immunohistology. Tissue sections (proximal jejunum and pan- Fig. 1. Q-PCR analysis of ZnT and Zip transcript abundance in mouse small creas) were fixed with 10% formalin in PBS, embedded in intestine. Each relative abundance was calculated by using 18S rRNA as the paraffin, cut as 5-␮M sections, and mounted. Incubation with normalizer. Values given as means Ϯ SEM (n ϭ 3). Transcript levels in response primary antibodies (10 ␮g͞ml) was followed by addition of to low (ZnϪ, Ͻ1mg͞kg) or high (Znϩ, 150 mg͞kg) Zn diet relative to adequate anti-rabbit IgG–Alexa 594 conjugate or anti-goat IgG–FITC (ZnN, 30 mg͞kg) Zn diet after the mice were fed for 21 days. (A) ZnT transcript conjugate (Molecular Probes). As negative controls, the respec- levels. MT mRNA is shown as an example of a Zn-responsive gene. (B) Zip transcript levels. , P Ͻ 0.05, compared with ZnN group. tive peptides that were used as the antigens were incubated with * the primary antibody before exposure to the tissue section. Ј Counterstaining of nuclei was performed with 4 ,6-diamidino- primer–probe set used in these experiments. The progression of 2-phenylindole. We used an Axiovert microscope (Zeiss) and Zip4 mRNA up-regulation during 21 days
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