NF-L, Mouse Mab MCA-DA2

NF-L, mouse mAb MCA-DA2 Applications Host Isotype Molecular Wt. Species Cross-Reactivity Ordering Information WB, IF/ICC, IHC Mouse IgG1 68kDa by Hu, Rt, Ms, Bo, Po, Ho Web www.encorbio.com SDS-PAGE Email [email protected] Phone 352-372-7022 Fax 352-372-7066 HGNC name: NEFL RRID: AB_2572362 Immunogen: Enzymatically dephosphorylated full length pig NF-L protein Format: Purified antibody at 1mg/ mL in 50% PBS, 50% glycerol plus 5mM NaN3 Storage: Store at 4°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles. Recommended dilutions: WB: 1:5,000. IF/ICC and IHC: 1:1,000. Western blot analysis of whole tissue lysates using mouse mAb Immunofluorescent analysis of rat frontal cortex section stained to NF-L, MCA-DA2, dilution 1:5,000 in green: [1] protein standard with mouse mAb to NF-L, MCA-DA2, dilution 1:500 in red, (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse and costained with chicken pAb to GFAP, CPCA-GFAP, dilution spinal cord. The strong band at about 70 kDa corresponds to NF- 1:5,000 in green. Following transcardial perfusion of rat with 4% References: L protein. paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, 1. Mersiyanova IV, et al. A new variant and free-floating sections were stained with above antibodies. The of Charcot-Marie-Tooth disease type 2 is MCA-DA2 antibody labels cell bodies and processes of pyramidal probably the result of a mutation in the neurons, as well as dendrites and axons of other neuronal cells, neurofilament-light gene. Am. J. Hum. while the GFAP antibody stains the network of glial cells. Genet. 67:37-46, 2000. 2. Edson KJ, Linck RW, Letourneau PC. Identification of a tektin-like protein associated with neurofilaments in the Background: Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, developing chick nervous system. J and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L is the Neurosci Res. 30:105-15 1991. neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68 kDa. 3. Bongarzone ER, et al. Two neuronal Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue cell lines expressing the myelin basic culture. NF-L antibody can also be useful in the diagnostics of neurofilament accumulations seen in many protein gene display differences in their neurological diseases, such as Lou Gehrig’s disease and Alzheimer’s disease. Mutations in the protein coding in vitro survival and in their response to region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease. This antibody has been glia. J Neurosci Res. 54:309-19 1998. sold by Millipore-EMD for many years under the catalog number Mab1615, and has been used in numerous 4. Evans J. et al. Characterization publications. The same antibody is also marketed by Abcam, Cell Signaling Technology, Novus, ThermoFisher of mitotic neurons derived from adult and many other companies. rat hypothalamus and brain stem. J. Neurophysiol. 87:1076-85 2002. FOR RESEARCH USE ONLY. NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE. Abbreviation Key: mAb—Monoclonal Antibody pAb—Polyclonal Antibody WB—Western Blot IF—Immunofluorescence ICC—Immunocytochemistry IHC—Immunohistochemistry E—ELISA Hu—Human Mo—Monkey Do—Dog Rt—Rat Ms—Mouse Bo—Cow Po—Pig Ho—Horse Ch—Chicken Dr—D. rerio Dm—D. melanogaster Ce—C. elegans Sc—S. cerevisiae Sa—S. aureus Ec—E. coli..

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