MAP1LC3B Antibody Cat

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MAP1LC3B Antibody Cat. No.: 7581 MAP1LC3B Antibody Immunohistochemistry of MAP1LC3B in human brain Immunofluorescence of MAP1LC3B in human brain tissue tissue with MAP1LC3B antibody at 5 μg/mL. with MAP1LC3B antibody at 20 μg/mL. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse, Rat HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%) MAP1LC3B antibody was raised against an 18 amino acid peptide near the amino terminus of human MAP1LC3B. IMMUNOGEN: The immunogen is located within the first 50 amino acids of MAP1LC3B. TESTED APPLICATIONS: ELISA, IF, IHC-P, WB September 29, 2021 1 https://www.prosci-inc.com/map1lc3b-antibody-7581.html MAP1LC3B antibody can be used for detection of MAP1LC3B by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. APPLICATIONS: Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. MAP1LC3B antibody is human, mouse and rat reactive. Multiple isoforms MAP1LC3B are SPECIFICITY: known to exist. MAP1LC3B antibody is predicted to not cross-react with MAP1LC3A or MAP1LC3C POSITIVE CONTROL: 1) Cat. No. 1303 - Human Brain Tissue Lysate 2) Cat. No. 10-301 - Human Brain Tissue Slide Predicted: 14 kDa PREDICTED MOLECULAR WEIGHT: Observed: 14 kDa Properties PURIFICATION: MAP1LC3B antibody is affinity chromatography purified via peptide column. CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: MAP1LC3B antibody is supplied in PBS containing 0.02% sodium azide. CONCENTRATION: 1 mg/mL MAP1LC3B antibody can be stored at 4˚C for three months and -20˚C, stable for up to STORAGE CONDITIONS: one year. Additional Info OFFICIAL SYMBOL: MAP1LC3B MAP1LC3B Antibody: LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1ALC3, Autophagy- ALTERNATE NAMES: related protein LC3 B ACCESSION NO.: NP_073729 PROTEIN GI NO.: 12383056 GENE ID: 81631 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References September 29, 2021 2 https://www.prosci-inc.com/map1lc3b-antibody-7581.html Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and plasticity (1). MAP1LC3B belongs to the MAP1 LC3 family and it includes 3 different light chains, LC1, LC2 and LC3 (2). MAP1LC3B is involved BACKGROUND: in formation of autophagosomal vacuoles (autophagosomes) (3). It is most abundant in heart, brain, skeletal muscle and testis. MAP1LC3B is essential for autophagy and associated to the autophagosome membranes after processing (4). 1) Mandelkow E and Mandelkow EM. Microtubules and microtubule-associated proteins. REFERENCES: Curr. Opin. Cell Biol. 1995; 7:72-81. 2) Fink JK, Jones SM, Esposito C, et al. Human microtubule-associated protein 1A (MAP1A) gene: genomic organization, cDNA sequence, and developmental and tissue-specific expression. Genomics 1996; 35:577-85. 3) Colecchia D, Strambi A, Sanzone S, et al. MAPK15/ERK8 stimulates autophagy by interacting with LC3 and GABARAP proteins. Autophagy 2012; 8:1724-40. 4) Kabeya Y, Mizushima N, Ueno T, et al. LC3, a mammalian homolog of yeast Apg8p, is localized in autophagosome membrane after processing. EMBO J. 2000; 19:5720-8. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 29, 2021 3 https://www.prosci-inc.com/map1lc3b-antibody-7581.html.

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