Anne-Calof-CV.Pdf

CURRICULUM VITAE - ANNE LEIGHTON CALOF (SERVICE AND RECENT GRANT ACTIVITY HIGHLIGHTED IN BLUE)

ADDRESS Department of Anatomy & Neurobiology Developmental & Cell Biology, and the Center for Complex Biological Systems University of California, School of Medicine Irvine, California 92697-1275 [email protected]

EDUCATION AND PROFESSIONAL EXPERIENCE 1978 B.A. in Biology/Psychology Reed College Portland, OR 1985 Ph.D. in Neurobiology (L.F. Reichardt, Mentor) Department of Physiology University of California, San Francisco School of Medicine San Francisco, CA 1985-1987 Postdoctoral Associate (T. Jessell, Mentor) Howard Hughes Medical Institute, Center for Neurobiology and Behavior Columbia University College of Physicians & Surgeons New York, NY 1987 Assistant Instructor Molecular Embryology of the Mouse course Cold Spring Harbor Laboratory Cold Spring Harbor, NY 1987-1990 Postdoctoral Research Associate/Instructor (D. Chikaraishi, Mentor) Tufts Univ. Sch. Med., Boston, MA 1991-1995 Assistant Professor Department of Biological Sciences University of Iowa, Iowa City, IA 1995-present Assistant, Associate, Full Professor (Step VI as of July 2018) Department of Anatomy & Neurobiology, University of California, Irvine, School of Medicine

2018-present Adjunct Professor Neurobiology and Cognitive Science Center National Taiwan University, Taipei, Taiwan

FELLOWSHIPS, HONORS, AWARDS 1978 Phi Beta Kappa, Reed College Chapter 1978-1981 Graduate Fellowship National Science Foundation 1981-1982 Graduate Opportunity Fellowship University of California 1988 Postdoctoral Fellowship Muscular Dystrophy Association 1989-1990 Postdoctoral Fellowship National Institutes of Health NRSA 1993-1996 Basil O'Connor Starter Scholar Research Award March of Dimes Birth Defects Foundation 2003-2004 Excellence in Teaching University of California, Irvine, School of Medicine 2005-2006 Excellence in Teaching University of California, Irvine, School of Medicine 2006-2007 Excellence in Teaching University of California, Irvine, School of Medicine 2008-2009 Faculty Mentor Award to Promote Diversity Office of Graduate Studies University of California, Irvine 2008-2013 Co-Director, Cornelia de Lange Foundation Center of Excellence in Research, CdLS Foundation USA 2009-2010 Excellence in Teaching for Best First Year Course Histology for MSIs, Anne L. Calof, Course Director University of California, Irvine, School of Medicine 2012-2013 Excellence in Teaching University of California, Irvine, School of Medicine 2013-2018 Co-Director, Cornelia de Lange Foundation Center of Excellence in Research, CdLS Foundation USA 2014-2015 Excellence in Teaching University of California, Irvine, School of Medicine 2015-2016 Excellence in Teaching University of California, Irvine, School of Medicine 2016 RARE Champion of Hope in Science Award, Global Genes Alliance in Rare Diseases

2018 Excellence in Teaching Award University of California, Irvine, School of Medicine

STATEMENT OF RESEARCH INTERESTS AND CONTRIBUTIONS TO SCIENCE The overall goal of my laboratory’s research is to understand how changes in cell lineage progression parameters and in the expression of diverse genes act in concert to direct normal development and, when development goes awry, to cause syndromic and non-syndromic birth defects. We are particularly interested in the nervous system, but we also study other systems such as heart, limb, visceral organs, and craniofacial structures. In many of our studies, we use mouse and zebrafish models of Nipbl-deficiency (the major cause of Cornelia de Lange Syndrome (CdLS)) as systems with which to understand this question – a question that is common to many ”transcriptomopathies” that are characterized by global dysregulation of gene expression arising as a consequence of an initial genetic lesion (e.g. NIPBL haploinsufficiency in CdLS, trisomy in Down Syndrome, MECP2 mutation in Rett Syndrome, etc.). Our goals are (1) to gain insight into general principles of developmental regulation by studying animal models of Nipbl-deficiency, and (2) to use these model systems to test therapeutic interventions for CdLS. Our studies on CdLS involve, in addition to me and the members of my laboratory, other investigators at UCI and around the world. Our colleagues at UCI include Arthur Lander, Thomas Schilling (who oversees the work on zebrafish that we pursue as part of our studies of CdLS), and Kyoko Yokomori (whose primary interest in CdLS is in chromatin structure and regulation of gene expression). The papers resulting from such studies are published collaboratively, with between two and four PIs and different members of the various laboratories taking authorships, as appropriate for a given study. My lab’s most recent studies of Nipbl-deficient mice have led to novel insights into the determinants of risk for the development of congenital heart disease (see Santos et al., 2016, PLoS Biology; and review by Gelb BD (The Hole and the Whole: Lessons from Manipulation of Nipbl Deficiency. PLoS Biol. 2016 Sep 8;14(9):e2000494. doi:0.1371/journal.pbio.2000494). In addition to molecular approaches to studying development and congenital disease, I have increasingly employed a systems biology approach in my laboratory’s studies. I serve as a Theme Leader in Control of Growth and Morphogenesis at UCI’s Center for Complex Biological Systems, a NIGMS-sponsored National Center for Systems Biology. In this capacity, I work with many investigators on interdisciplinary research projects that integrate computational modeling, genomics, and high-throughput data gathering integrated with experimental developmental biology and genetics. My lab’s research in this area has mainly been focused on understanding how signaling systems participate in feedback regulation of stem and progenitor cells and the effects of such feedback on morphogenesis, patterning, and regulated growth of different tissues. The systems for which our contributions have been most significant are the olfactory epithelium, neural retina, and gustatory epithelium of the tongue, although we currently have a project to study neocortex as well. During the review period, we have published a number of studies that combine wet-bench experimentation with extensive mathematical analysis and computational modeling, as well as some papers that are purely theoretical. These studies are published collaboratively, with multiple PIs and members of the participating research groups taking authorships as appropriate for a given study. Our most recent work, published in 2016 in PLoS Computational Biology, is a theoretical exploration of the ways in which feedback effects on stem cell self-renewal can serve as a driving force in morphogenesis (Kunche et al., 2016, PLoS Computational Biology). I consider my major contributions to science to be in the following areas: 1. Vertebrate animal models to understand the origins of sporadic and syndromic birth defects: Cornelia de Lange Syndrome (CdLS) is a multi-system birth defects syndrome caused, in most cases, by haploinsufficiency for NIPBL, which encodes a cohesin regulatory protein. We helped identify NIPBL as the causative gene in most cases of CdLS, and developed the first mouse and zebrafish

models for Nipbl-deficiency. Our studies of these model systems led to the understanding that birth defects observed in CdLS can be attributed to global transcriptional dysregulation in Nipbl-deficient tissues. Since birth defects that arise in CdLS are also common in the general population, these models are helping us understand the etiology of sporadic birth defects, and in particular have recently contributed to our understanding of the complexity of risk determinants for congenital heart defects. Representative publications are: Krantz et al., 2004 (Nature Medicine); Kawauchi et al., 2009 (PLoS Genetics); Muto et al., 2011 (PLoS Biology); Muto et al., 2014 (PLoS Genetics); Santos et al., 2016 (PLoS Biology). 2. Understanding how neural stem cells are regulated by secreted signaling molecules: What signals govern the behavior of neural stem cells, their differentiation into committed progenitors and, subsequently, into terminally-differentiated neurons? Do different signaling molecules oppose one another in regulating progression of cells through neural lineages? What are the mechanisms by which these signals act? Do cells within a lineage contribute to building their own niche, and if so, how? The systems for which our contributions have been most significant in addressing these questions are the olfactory epithelium, neural retina, and gustatory epithelium of the tongue. Representative publications are: DeHamer et al., 1994 (Neuron); Shou et al., 1999 (Nature Neuroscience); Wu et al., 2003 (Neuron); Kim et al., 2005 (Science); Kawauchi et al., 2005 (Development); Beites et al., 2009 (Development); Kawauchi et al., 2009 (Development); Gokoffski et al., 2011 (Development). 3. Feedback and the regulation of proliferative control and spatial patterning: How do neural stem and progenitor cells know when to stop dividing and differentiate? Does feedback play a role? Can mathematical models help us understand how feedback might achieve growth control? Evidence from studies of induced neurogenesis and regeneration of the olfactory epithelium suggested to us that differentiated neurons provide negative feedback signals that regulate proliferation and size control. We identified, for the first time, a signaling molecule mediating negative feedback of neurogenesis (GDF11). We discovered that negative feedback is important in determining not only neuron number, but also stem cell fate; and in the process determined that a single signaling mrotein – GDF11 – could exert these actions in both neural retina and OE. We used such findings as the basis for developing a model of cell lineage progression that we explored to determine how feedback confers robustness to different perturbations in regenerating olfactory epitheliium; how it controls progress of stem cells through the hierarchy of commitment and terminal differentiation; and how it contributes to morphogenesis and spatial patterning of tissues. Exploration of models also led us to identify additional feedback loops important in directing lineage branching and fate acquisitions by stem cells, as well as patterning of stem cell domains. Representative publications are: Wu et al., 2003 (Neuron); Kim et al., 2005 (Science); Lander et al., 2009 (PLoS Biology); Beites et al., 2009 (Development); Gokoffski et al., 2011 (Development); Kunche et al., 2016 (PLoS Computational Biology). 4. Highly-characterized tissue culture systems for studies of neurogenesis and neuronal differentiation: I was motivated to develop these systems by the need to understand the actions of molecular signals on neurons and the stem and progenitor cells that give rise to them in the controlled environment of tissue culture. As a graduate student, I developed methods for purifying spinal motor neurons by fluorescence-activated cell sorting. As a postdoc, I developed a tissue culture system for mouse olfactory epithelium (OE) in which division and differentiation of stem and progenitor cells takes place, which allowed me to characterize the progression of neurogenesis. Later, my own laboratory developed a means to purify OE stem cells. These studies not only provided useful tissue culture systems for the neuroscience community, they also enabled us to uncover specific activities of laminin subunits in promoting adhesion, axon outgrowth, and cell migration; and to obtain direct evidence for the existence of feedback inhibition of neurogenesis, a finding that spurred many

subsequent studies by us and our collaborators. Representative publications are: Calof and Reichardt, 1984 (Developmental Biology); Calof and Chikaraishi, 1989 (Neuron); Calof et al., 1994 (Neuron); Mumm et al., 1996 (Proc. National Acad. Sci. USA).

FOUNDATIONS, PROFESSIONAL SOCIETIES, AND ADVISORY PANELS Cornelia de Lange Syndrome Foundation/World CdLS Federation (Research Advisory Committee and Board of Directors, 2003-2008; International Scientific Advisory Committee, 2005-present; Clinical Advisory Board 2003-present; Advancing Clinical Trials Committee, 2015-present) Society for Neuroscience (Session Chair, Cell Lineage and Determination III, SFN Annual Meeting, 1994; Session Chair, Genesis of Neurons and Glia I, SFN 27th Annual Meeting, 1997; Communications and Chapters Committee 1996-2002 [Chair, 1998-2002]; Committee on Committees 2002-2004; Government & Public Affairs Committee, 1998-2005) Association for Chemoreception Sciences (Co-organizer, Workshop on Olfactory Cell Cycle Analysis, AChemS Annual Meeting, 1996; Session Chair, “Neurogenesis and Cell Death in the Olfactory Epithelium”, 1997 International Symposium on Olfaction and Taste/AChemS, San Diego, CA; Symposium Co-Organizer, “Stem cells in sensory epithelium development and regeneration”, 2009 International Symposium on Olfaction and Taste, San Francisco, CA) Society for Developmental Biology; American Society for Cell Biology; Gordon Conference on Neural Development (1994, 1996, 2000) External Review Panel, NIH Program Project "Development and Regeneration in Vertebrate Chemoreception", University of Cincinnati Medical Center, March 1993 Neurology B2 Study Section, NIH/CSR, Ad Hoc Member 1994-1996; Regular Member, 1996-1998 Member, Special Emphasis Panel for RO3 applications, National Institute on Deafness and Other Communication Disorders, NIH, March 1995 External Review Panel, NIH Program Project "Development and Regeneration in Vertebrate Chemoreception", University of Maryland School of Medicine, June 1997 Regular Member, Molecular Cellular Developmental Neurobiology 6 Study Section, Center for Scientific Review, National Institutes of Health, 1998-1999 External Grant Reviewer, Department of Defense Congressionally-Directed Medical Research Program (CDMRP), Defense and Health-Related Research Program (DHRP), 2000, 2003 External Grant Reviewer, The Wellcome Trust, 2000 Scientific Advisory Council, House Ear Institute, Los Angeles, California, 2001 Reviewer, NIH/CSR Neurogenesis and Cell Fate Study Section, June, 2005 Reviewer, NIH/CSR Developmental Brain Disorders and Clinical Neuroscience Study Section, June 2006 Reviewer, Swedish Research Council, "Basic Mechanisms of Disease" panel, 2008 Reviewer, Swiss National Research Programme, “Stem cells and regenerative medicine” mechanism (NFP 63), 2009 Autism Workshop Member, California Institute for Regenerative Medicine (CIRM), 2009, San Francisco, CA Reviewer, Human Frontiers in Science Short Term Fellowship Programme, 2010 5

Reviewer, Medical Research Council, United Kingdom, 2010-2011 Regular Member, NIH/CSR, Development 1 Study Section, 2007-2011 Editorial Board Member, BMC Biology (2012-present) Member, NIH/CSR ZRG1 F05-D (21) Cell Biology, Developmental Biology, and Bioengineering Fellowship Special Emphasis Panel, 2013 Reviewer, New York State Health Dept. and the Empire State Stem Cell Board, NYSTEM Neurobiology 1 Panel, 2013 Reviewer, NICHD Special Emphasis Panel ZHD1 DSR-M BA, Human Structural Birth Defects, 2014 2015, Reviewer, NICHD SEP 2016/01 ZHD1 DSR-L (BA)1, Human Structural Birth Defects Reviewer, NIH/CSR Application Comparison Pilot Study, 2015-2016 2016, External referee for Biomedical Research, Fondazione Pisa, Italy 2016, Reviewer, NIA Special Emphasis Panel, ZAG1-ZIJP(J1) National Institute on Aging 2018, Member, NIH/CSR Special Emphasis Panel (F05-U), Cell Biology, Developmental Biology, and Bioengineering Fellowship Special Emphasis Panel (March 2018, Bethesda, MD) 2018, CME Planning Committee and Session Chair, Eighth Biennial Cornelia de Lange Syndrome Scientific and Educational Symposium, June 27-28, 2018, Minneapolis, Minnesota 2018, Member, 2018 NIH/NICHD Review Panel, ZHD1 DSR-Y (92), NICHD T32 Training Grant Awards (PA16-152) (November 2018, Bethesda, MD)

MEMBERSHIPS IN SCIENTIFIC/PROFESSIONAL SOCIETIES AND FOUNDATIONS Society for Neuroscience Society for Developmental Biology American Society for Cell Biology Association for Chemoreception Sciences (active membership ceased 2016) Genetics Society of America

PATENTS AND COPYRIGHTS US Patent Application, Filing Date May 30, 2006 (supercedes US Provisional Patent Application No. 60/685,630) "Compositions and Methods for Treatment of Neural Disorders Using Transforming Growth Factor- beta Superfamily Proteins and Their Antagonists" Inventors: Anne L. Calof (with Joon Kim, and Shimako Kawauchi)

US Patent Application Filing Date September 29, 2006 (supersedes U.S. provisional patent application 60/722695) "Compositions and Methods Relating to Cornelia de Lange Syndrome" Inventors: Arthur D. Lander and Anne L. Calof

WRITING AND EDITORIAL ACTIVITIES FOR PROFESSIONAL JOURNALS Trends in Neurosciences, "Journal Club" member (invited writer for Research News), 1990-1993 6

Issue Editor, NeuroProtocols 3: Immortalizing Neural Cells (Academic Press, 1993) Editorial Board, BMC Biology, 2010 - present Reviewer for the following journals (in addition to BMC Biology): Developmental Biology; Journal of Cell Biology; Neuron; Development; Neuroscience; J. Histochem. Cytochem.; J. Comp. Neurol.; J. Neurosci.; Eur. J. Neurosci.; Dev. Dynamics; Experimental Neurology; Science; J. Neurobiol.; Mol. Cell. Neurosci.; Nature Neuroscience; Micros. Res. Tech.; Neuron; Stem Cells; PLoS Biology; Neuroscience Letters; PLoS One; Developmental Cell, Developmental Dynamics, PLoS Genetics; Brain Research, Frontiers in Pediatrics

RESEARCH GRANT SUPPORT (2004-present, in chronological order; recent activity highlighted in blue)

Funding Source Number or Title Calof’s Role Amount Date Span of (name of PI or (TC) Award Director if other than Calof) NIH R01-DC003583 PI $1,703,441 4/03 – 3/10 Feedback regulation of neurogenesis in mammals

NIH R01-DC003583 PI $50,000 10/03 – 9/04 Feedback regulation of neurogenesis in mammals (Administrative Supplement for Generation of Targeted Mouse Mutations)

NIH P01-HD38761 Project Leader $935,343 6/00 –5/07 (Lander, Program (Calof director) project only) Positive and Negative Regulation of Neurogenesis by BMPs

UCI IRU in Hearing N/A PI $8,000 2002-2004 and Speech Sciences Molecular Genetic Analysis of Signaling Molecules in Ear Development

March of Dimes 1-FY05-138 PI $294,507 6/05-9/08 Birth Defects Foundation Autoregulation of Neurogenesis by GDF11 in the Developing Central Nervous System

UCI IRU in Hearing Pilot Grant Co PI with $4,000 2006 and Speech Sciences Leonard Kitzes and postdoc Shimako Kawauchi Investigations of auditory dysfunction in a mouse model of Cornelia de Lange Syndrome

UCI Center for Opportunity Award MPI (one of two) $11,000 2007-2008 Complex Biological Systems Developing a Stochastic Model of Neuronal Stem Cell Aging

UCI Center for Opportunity Award MPI (one of two) $11,000 2008-2009 Complex Biological Systems Computational Modeling of Taste Papillae Patterning using the Classic Turning-Meinhardt Model

Foundation Fighting BR-CMM-0507- PI $100,000 6/07-5/10 Blindness 0380-UCI approximate annual direct costs Role of the Endogenous GDF11-FST Neurogenic Control System in the Pathophysiology and Potential Treatment of Retinal Degenerative Disease

UCI Center for Opportunity Award MPI (one of two) $11,000 2009-2010 Complex Biological Systems Cell Lineages as a Progression of Stochastic Dynamical Systems

UCI Center for Opportunity Award MPI (one of two) $11,000 2011-2012 Complex Biological Systems Role of Localized Cell Proliferation in Branching Morphogenesis of the Olfactory Epithelium and Nasal Cavity

NIH P50-GM076516 Theme B Leader $2,199,700 8/07-9/12 (amount shown is for Theme B only)

Systems Biology of Morphogenesis and Spatial Information Flow

NIH P01 HD-52860 Co-Project Leader, $1,748,853 4/06-3/11 Project 2 (amount (Krantz) shown is for this project only) Vertebrate Animal Models of Cornelia de Lange Syndrome This is one of three projects in a trans-institutional program project grant. The aims are to generate and study mouse and zebrafish models of Cornelia de Lange Syndrome. I am one of 3 Co-PIs on this project.

NIH 3P01 HD-52860- Co-Project Leader $142,719 9/08-2/11 03S1 (amount shown is for this project (Krantz) only) Vertebrate Animal Models of Cornelia de Lange Syndrome (minority supplement) 8

This is one of three projects in a trans-institutional program project grant. The aims are to generate and study mouse and zebrafish models of Cornelia de Lange Syndrome. I am one of 3 Co-PIs on this project.

NIH 5P01 HD-52860 - Co-Project Leader $159,760 9/09-9/11 04S1 (amount shown is for this project (Krantz, I.), only) Vertebrate Animal Models of Cornelia de Lange Syndrome (ARRA administrative supplement) This is one of three projects in a trans-institutional program project grant. The aims are to generate and study mouse and zebrafish models of Cornelia de Lange Syndrome. I am one of 3 Co-PIs on this project. This is an ARRA supplement specifically for personnel.

NIH P01-HD052860-06 Project Leader, $4,915,528 4/11-3/17 Project 2(amount of which (Krantz, I., shown is for this approx Children’s project only $1,950,000 Hosp. of PA.) for UCI project Vertebrate Animal Models of Cornelia de Lange Syndrome (competitive renewal) This is one of three projects in a trans-institutional program project grant. The aims are to generate and study mouse and zebrafish models of Cornelia de Lange Syndrome. I am one of 3 Co-PIs on this project.

NIH P50-GM076516- Theme B Leader $2,460,459 9/12-8/18 06 (amount shown is for Theme B only Systems Biology of Morphogenesis and Spatial Information Flow (competitive renewal) This represents one of three large, multi-disciplinary research themes (Theme B, Epithelial Growth Control) of a NIGMS Center Grant in Systems Biology, of which Dr. Arthur Lander is center director. Theme B concentrates on mathematical and computational modeling of epithelial growth control. Calof is Theme B Leader, and directs the efforts of the theme, which also has seven other faculty.

NIH R21 HD062951 Collaborator (role) $413,100 2/10-1/12 (Yokomori, K) Role of Cohesin in Developmental Gene Regulation A collaborative project among multiple investigators to study the role of cohesion in the regulation of transcription of developmentally important genes.

Cornelia de Lange 200246 PI $15,000 9/14 – 8/15 Syndrome Foundation Therapeutic Intervention in a Mouse Model of CdLS The goal of this project is to identify whether a proposed pharmacological treatment of the most common underlying genetic defect in CdLS is feasible and will succeed in mammals

UCI Devel. Biology Pilot Grant One of two MPIs $10,000 10/14-10/15 Center

Development of CRISPR-mediated knock-in mice expressing a Halo-tagged Nipbl from its endogenous locus

NIH R21- HD078849- Co-Investigator $419,921 05/14 - 04/16 01 (Yokomori, K) Nipbl Haploinsufficiency in Cornelia de Lange Syndrome

Scholar Rock, Inc SRI-206626 PI $5,328 8/16-9/16

Provision of Specialized Tissue to Scholar Rock, Inc. for Analysis with Proprietary Antibodies

University of 2015-UTUCI Supervisor $100,000 6/15-5/17 Tsukuba Tsukuba University Liaison Office This grant provides salary support for a senior member of the Calof Lab, Dr. Shimako Kawauchi, to provide liaison services between the CCBS at UCI and Tsukuba University in Japan

March of Dimes #1 – FY15-337 PI $247,000 06/15-5/18 Gene Expression Signatures to Assess Treatment Strategies for Developmental Disorders Using a Conditional/Invertible Mouse Model

NIH R01 NS095355-01 MPI (one of three) $2,120,107 09/15-6/20 Spatial Dynamics of Tissue and Organ Size Control

NIH R01HL138659-01 MPI (one of two) $2,124,918 08/17 – 5/21 Investigating the multifactorial origins of congenital heart disease

TRAINING & SHORT-COURSE GRANT PARTICIPATION at UCI (most recent activity highlighted in blue) NIH T32-HD07029 One of 19 $145,650 9/00 – 8/05 (Bryant, P.) training supervisors Developmental Mechanisms Underlying Congenital Defects

NIH T32-NS07486 One of 12 $1,072,339 5/01-5/06 (Steward, O.) training supervisors Postdoctoral Training Program in Neural Regeneration

NIH T32-NS07444 1 of 16 training $596,800 07/03-06/09 (Lander, A.D.) supervisors

Training Program in Cellular and Molecular Neuroscience

NIH T32 CA09054 (Fan, 1 of 35 training $2,292,957 4/02- 4/06 Hung) supervisors A Training Program in Carcinogenesis

NIH T32 HD060555 1 of 26 training $1,244,465 5/09-4/14 supervisors Systems Biology of Development

NIH T32 EB009418 1 of 37 training $1,112,030 4/09-3/14 supervisors Mathematical, Computational and Systems Biology

NIH R25-GM096989 One of 16 co- $88 6,124 4/11-3/16 (Wan and investigators Lander) A National Short Course in Systems Biology: Tackling Spatial Dynamics in Cells and Tissues This grant established an open-access annual 3-week course at UCI for training scientists with an interest in learning skills relevant to systems biology. NIH T32NS082174 1 of 21 training $771,851 06/13-06/18 supervisors

Training Program in Stem Cell Translational Medicine for Neurological Disorders

NIH T32 EB009418 1 of 43 training $1,237,683 04/16-3/20 supervisors Mathematical, Computational and Systems Biology (competitive renewal)

NIH R25GM126365 One of 27 co- $1,469,184 09/17-07/22 investigators

Systems Biology: A Foundation for Interdisciplinary Careers

GRANTS APPLIED FOR BUT NOT FUNDED (August 1, 2017 – present)

The following was submitted September 5, 2017 as a subcontract of an SBIR2 grant proposal by Recursion Pharmaceuticals to the NIH, but was not funded.

X NIH- R43 HD084025 MPI of UCI $403,335 03/18-02/20 SBIR2 Subcontract (proposed (one of two) subcontract total costs)

Application of an efficient drug repurposing platform to identify treatments for Cornelia de Lange Syndrome

SERVICE, UNIVERSITY OF IOWA Panelist and Presenter, "Funding for Research, Scholarly and Creative Activity", College of Liberal Arts Orientation for Newly Appointed Faculty (Organizer: Dean K. Gfeller), August 19, 1993 Co-Chair, Organization Committee for First Annual Neuroscience at Iowa Conference, Neuroscience Ph.D. Program, 1993 Seminar Committee, Department of Biological Sciences, 1992-1994 Member, Graduate Programs in Neuroscience and Biological Sciences, 1992-1995

SERVICE, UNIVERSITY OF CALIFORNIA, IRVINE Graduate Committee, Dept. of Anatomy and Neurobiology, 1995-1999 Chair, Seminar Committee, Dept. of Anatomy and Neurobiology, 1995-1998 Ad Hoc Member, Senate Committee on Research, School of Medicine Academic Senate, 1996 Interviewer and Mentor, M.D.-Ph.D. Program, School of Medicine, 1996- present UCI Research Infrastructure Group Roundtable “Stress Project” (National Science Board /Government-University-Industry Research Roundtable), June, 1996 Senior Human Geneticist Search Committee, School of Medicine, 1996-1997 Committee to Review the School of Medicine, 1996 Committee on the Advancement of Women Faculty, School of Medicine, 1997 Curriculum Committee, MBGB Graduate Program, Schools of Biological Sciences and Medicine, 1997-1999 M.D.-Ph.D. Program Steering Committee, School of Medicine, UCI, 1998-2001 Co-organizer, Department of Developmental and Cell Biology Seminar Series, 1998-2013 Faculty Co-Advisor and Organizational Committee Member, "Building and Re-Building the Brain Symposium" at the Beckman Center for the National Academy of Sciences of UCI, 1999-2000. Faculty Mentor, MBGB Graduate Gateway Program, 1999-2009 (Admissions Committee 2007- 2008) Molecular Neurobiology Track Coordinator, MBGB Graduate Program, 2000-2006 Molecular Medicine Search Committee, Dept. of Medicine, 2001-2002 Core Group, Genetic Epidemiology Research Institute, 2001-2002 Mentor and Research Supervisor, UCI Minority Undergraduate Biomedical Research Support Program (MBRS) and BRIDGES to the Baccalaureate Program for Diversity Students, School of Biological Sciences, 2002-2008 Selection Committee, FST Student Travel Awards, School of Biological Sciences, 2002, 2003 Admissions Committee, MBGB Graduate Program, Schools of Biological Sciences and Medicine, 1999-2003 Ad Hoc Committee for Promotion to Tenure, UCI Academic Affairs, 2004 Faculty Mentor, Interdepartmental Neuroscience Graduate Program, 2004-present

Mentor and Research Supervisor, UCI PREP Post-Baccalaureate, Pre-Ph.D. Program for Diversity Students, School of Biological Sciences, 2005-2010 Interviewer, UC Irvine MSTP Program admissions, School of Medicine, 2005-present Campus-wide Search Committee, Stem Cell Initiative, 2005 Member, School of Medicine Academic Assembly, 2005-2007 Co-Director, SoCal Consortium for Eye Regeneration Research, UC Irvine, UC Santa Barbara, UC Riverside, and UC Los Angeles (2006-2007) Co-Organizer, Roundtable Workshop on Cornelia de Lange Syndrome and University-Foundation Interactions in Birth Defects Research, School of Biological Sciences and School of Medicine, 2006 Member and Theme Leader, Center for Complex Biological Systems, a NIGMS National Center in Systems Biology, 2007-present Mathematical, Computational and Systems Biology Gateway Graduate Program (Biology Bootcamp Lecturer/Co-organizer), 2008-present First-Year Graduate Student Advisor, Mathematical, Computational and Systems Biology Gateway Graduate Program, 2008-present Educational Support Committee, UC Irvine School of Medicine, 2008-2010 (joint with Curriculum and Education Policy Committee on a quarterly basis and SoM Academic Senate, 2008-2010) Faculty Mentor, CMB Graduate Gateway Program, 2009- present Council of Mentors, Institute for Clinical and Translational Science, School of Medicine; 2009- present Co-organizer, “System Biology of Stem Cells” Symposium (co-sponsored by Center for Complex Biological Systems, Sue and Bill Gross Stem Cell Center, and California Institute for Regenerative Medicine), Beckman Center for the National Academy of Sciences of UCI, 2010 DECADE Mentor: 2011-2015 School of Medicine. (The UCI DECADE (Diverse Educational Community and Doctoral Experience) program, is a University-wide, federally-funded (US Department of Education) program to advance diversity in faculty, staff, and students at the University of California. Library Committee, School of Medicine (Member, 1996-2001, 2003-2005, 2011-2016; Chair, 2011- 2016) Executive Committee, School of Medicine, 2011-2016 Co-organizer and Platform Speaker, 2nd International Symposium on Systems Biology of Stem Cells, Arnold and Mabel Beckman Center for the National Academies of Science and Engineering, Irvine, California, June 9-11, 2013 Mentor and Research Supervisor, SURF (Summer Undergraduate Research Fellowship) program, which supports diversity students that UCI Graduate Division seeks to recruit to its Ph.D. programs, 2014 UC Irvine Divisional Assembly Representative, for the School of Medicine, 2014-2016 Departmental Representative for Anatomy & Neurobiology to the COHS Representative Assembly, 2015-2017 Ad Hoc Committee to Review Department Chairs for UCI School of Medicine, 2016 13

Member, UC Irvine Council on Research, Computing & Libraries (CORCL), 2015-present CORCL Subcommittee on ORUs and SRPs, 2016-2017 Member, UCI Research Cyber Infrastructure Center (RCIC) Advisory Committee, 2017-present Departmental Representative for Anatomy & Neurobiology to the COHS Representative Assembly, 2017-2019 COHS Representative to the Irvine Divisional Senate, 2017-2019 UCI Institutional Animal Care and Use Committee (IACUC), Member, 2018-2021

RESEARCH CENTER AFFILIATIONS, UC IRVINE 1995-present Faculty Affiliate, Developmental Biology Center, 1996-present Faculty Affiliate, Chao Comprehensive Cancer Center, and Cancer Research Institute 2001-present Faculty Affiliate, UCI Center for Complex Biological Systems Theme Leader, 2006- present 2002-present Faculty Affiliate, UCI Center for Hearing Research 2006-present Member, UCI Sue and Bill Gross Stem Cell Research Center

TEACHING ACTIVITIES PRIOR TO APPOINTMENT AT UC IRVINE COLD SPRING HARBOR LABORATORY, NEW YORK 1987 Assistant Instructor Molecular Embryology of the Mouse course 1988 Lecturer in Developmental Neurobiology Molecular Embryology of the Mouse course

TUFTS UNIVERSITY SCHOOL OF MEDICINE, BOSTON, MASSACHUSETTS 1989 Lecturer in Developmental Neurobiology Graduate Program in Neuroscience

UNIVERSITY OF IOWA MEMBERSHIPS IN GRADUATE PROGRAMS , UNIVERSITY OF IOWA 1991-1995 Faculty Member Graduate Programs in Biology and Neuroscience

UNDERGRADUATE BIOLOGY HONORS STUDENTS SUPERVISED, UNIVERSITY OF IOWA 1995 Rachel Davis 1994 Jeff Mumm, Rachel Davis 1993 Melinda DeHamer, Sara Whitehead, Murali Adusumalli, Jeff Mumm 1992 Sara Whitehead, Melinda DeHamer 14

GRADUATE STUDENTS SUPERVISED, UNIVERSITY OF IOWA 1995 Phu Tran, Hui-Hui Dai, Adam Jenkins, Jianyong Shou 1994 Brian Limberg, Phu Tran, Angela Loihl, Stefanie Jurk, Kai Jiao 1993 Jae-Gun Yoon, Jisue Lee, Jeff London-Davidson, Brian Limberg 1992 Jill Bayliss, Dawn Wamboldt, Ron Paliwoda

POSTDOCTORAL FELLOWS SUPERVISED, UNIVERSITY OF IOWA 1993-1995 J. David Holcomb, M.D. (currently Director, Holcomb Facial Plastic Surgery, Sarasota, FL) 1993-1995 Nobuko Hagiwara, Ph.D. (currently Assistant Professor, Division of Cardiovascular Medicine/Rowe Program of Genetics, University of California, Davis, CA)

COURSES TAUGHT, UNIVERSITY OF IOWA 1995 Instructor, Honors Seminar in Biology Department of Biological Sciences (2:198) The University of Iowa 1993-1994 Instructor, Molecular Developmental Neurobiology Department of Biological Sciences [2:255] The University of Iowa 1992-1994 Instructor, Introduction to Developmental Biology Department of Biological Sciences [2:104] The University of Iowa

TEACHING ACTIVITIES, UNIVERSITY OF CALIFORNIA AT IRVINE

MAJOR TEACHING RESPONSIBILITIES DURING THE REVIEW PERIOD (2004-2017) HUMAN MICROSCOPIC ANATOMY (HISTOLOGY) Anatomy 503; School of Medicine (Fall, Winter, Spring quarters) Course Director or Key Faculty (varies by year): CALOF, A. Enrollment: 90-104 students (Medical Students, MS1)

DEVEL NEUROBIOLOGY (Fall, Winter, Spring, Summer quarters) Bio Sci 199, Sec. 295, 1-5-unit Research; School of Biological Sciences Instructor: CALOF, A. Enrollment: up to 9 students (Undergraduate)

ANATOMY RESEARCH (Fall, Winter, Spring quarters) Anatomy 200, 2-12-unit Research; School of Medicine Instructor: CALOF, A. Enrollment: up to 3 students (Graduate)

DEV NEUROBIOLOGY (Fall, Winter, Spring quarters) Dev Bio 200A, Sec. 9, 2-12-unit Research; School of Biological Sciences Instructor: CALOF, A. Enrollment: 1- 3 students (Graduate)

ADV TOP IN CELL BIO (Fall quarter; ) Dev Bio 206A, Sec. A, 2-unit Seminar; School of Biological Sciences Instructor: CALOF, A. Enrollment: 20-30 students (varies by year) (Graduate)

DEVEL & CELL BIOL (Fall quarter) Dev Bio 290A, Sec. A, 2-unit Colloquium; School of Biological Sciences Instructor: CALOF, A. Enrollment: 20-40 students (varies by year) (Graduate)

MEMBERSHIPS IN GRADUATE PROGRAMS , UCI 1995-1997 Graduate Program in Anatomy and Neurobiology; 1995-present M.D.-Ph.D. Program, College of Medicine 1997-present Graduate Program in Molecular Biology, Genetics, & Biochemistry (Cellular & Molecular Biology as of 2009) M.D.-Ph.D. Program, College of Medicine 2004-present Interdepartmental Neuroscience Program 2007-present Graduate Program in Mathematical, Computational & Systems Biology

PH.D. ADVANCEMENT AND PRELIMINARY EXAMINATION COMMITTEES, UCI 1996 Amy Dawson, Dept. of Developmental & Cell Biology (Member) 1997 Robert Chen, Dept. of Developmental & Cell Biology (Member) 1997 Kevin Hoover, Dept. of Developmental & Cell Biology (Member) 1997 Jianyong Shou, Dept. of Anatomy and Neurobiology (Chair) 2003 Candice Crocker, Dept. of Anatomy & Neurobiology (Chair) 2003 Joon Kim, Dept. of Anatomy & Neurobiology (Chair) 2006 Eric Pendleton, Dept. of Anatomy & Neurobiology (M.S. 2006)(Chair) 2007 Kimberly Gokoffski, Dept. of Developmental & Cell Biology (Chair) 2009 Piper Hollenbeck, Dept. of Anatomy & Neurobiology (Chair) 2010 Rosaysela Santos, Dept. of Anatomy & Neurobiology (Chair) 2014 Marissa Machietto, Dept. of Developmental & Cell Biology (Member) 2014 Joshua Gervin, Dept. of Developmental & Cell Biology (Member) 16

2016 Phoebe Valdes, Dept. of Developmental & Cell Biology (M.S. 2016) (Chair) 2016 Andrew Dolinko, Dept. of Developmental & Cell Biology (transferred 2017) (Member) 2017 Roy Zhao, Mathematical, Computational, & Systems Biology Graduate Program (Member) 2018 Roy Zhao, Mathematical, Computational, & Systems Biology Graduate Program (Member)

UNDERGRADUATE STUDENT RESEARCHERS SUPERVISED, UCI (# indicates diversity student) BIO 199 UNDERGRADUATE RESEARCHERS 1996 Brian Lin, Sharan Katra 1997 Sharan Katra, Andrew Wang, Cambria Hembry, Raj Gopalakrishnan 1998 Andrew Wang, Cambria Hembry, Raj Gopalakrishnan, Sinda Osburn 1999 Raj Gopalakrishnan, Ragui Sedeek, Seong Choi, Daniel Navandzadeh 2000 Raj Gopalakrishnan, Seong Choi, Daniel Navandzadeh, Sylvia Jaramillo#, Alice Ly 2001 Daniel Navandzadeh, Sylvia Jaramillo#, Alice Ly, Natalia Mangubat 2002 Sylvia Jaramillo#, Alice Ly, Huong Tran, Rosaysela Santos# 2003 Sylvia Jaramillo#, Alice Ly, Huong Tran, Connie Tsai, Rosaysela Santos#, Candice Rubio 2004 Rosaysela Santos#, Candice Rubio, Vu Tran, Aida Brush#, Annie Espinosa#, Dan Phan 2005 Dan Phan, Catherine Sung, Annie Espinosa#, Stephen Melgarejo#, Devin Aria# 2006 Devin Arias#, Stephen Melgarejo#, Clint Young, Alice Quach, Neel Pandya, Bedro Jin 2007 Clint Young, Erik Goka, Bedro Jin, Neel Pandya, Joe McKelley, Steven Claessens, Garrett Johnson, Michelle Hoang, Shabnam, Ghazizadeh, Christina Cheng, Teresa Ung, Michelle Hoang, Aileen Alvarez, Theresa Chua, Jenny Lee, Tracy Clevenger 2008 Shabnam Ghazizadeh, Christina Cheng, Teresa Ung, Michelle Hoang, Aileen Alvarez, Theresa Chua, Teresa Ung, Jenny Lee, Bedro Jin, Samir Quarashi, Shirley Kim, Tracy Clevenger 2009 Jeffry Wu, Teresa Ung, Aileen Alvarez, Theresa Chua, Jenny Lee, Samir Quarashi, Shirley Kim, Tracy Clevenger, Ceyda Yaramanoglu, Napoleon Greene#, Randy Luu, Rachel Asprer 2010 Jeffry Wu, Tracy Clevenger, Ceyda Yaramanoglu, Napoleon Greene#, Randy Luu, Samir Qurashi, Tritia Schostak, Teresa Ung, Mona Yazdi, Jared Olivo 2011 Napoleon Greene#, Michael Liang, Randy Luu, Ceyda Yaramanoglu, Tritia Schostak, Mona Yazdi, Jared Olivo, Andrew Nguyen, Kalvin Nguyen, Salvador Deniz# 2012 Michael Liang, Alexander Hardy, Mona Yazdi, Andrew Nguyen, Kalvin Nguyen, Salvador Deniz#, Harrison DiStefano, Abhinav Mohan, Laura Ochikubo, David Razo# 2013 Rachel Phan Banh, Harrison DiStefano, Sameer Kandula, Abhinav Mohan, Laura Ochikubo, David Razo#, Jeremy Sharma, Louise Villagomez, Tyler Anderson 2014 Jeremy Sharma, Louise Villagomez, Tyler Anderson, Sameer Kandula, Abhinav Mohan, Laura Ochikubo, Phoebe Yam, Abhay Mahajan, Hojae Choi, Esteban Kim, Abigail Reeser 2015 Hojae Choi, Qumber Ali, Esteban Kim, Abhay Mahajan, Henry Chang, Rebecca Varghese 2016 Hojae Choi, Qumber Ali, Esteban Kim, Jocelyn Ha, Henry Chang, Rebecca Varghese, James Thien Tran, Daniel Gilchrist, Amanda Hidajat, Amanda Nyam 17

2017 Qumber Ali, Jocelyn Ha, Henry Chang, Rebecca Varghese, Daniel Gilchrist, Amanda Hidajat, Amanda Nyam, Hema Monica Sharda 2018 Amanda Hidajat, Amanda Nyam, Hema Monica Sharda, Daniel Gilchrist, Lorelle Meidt, Han Nguyen 2019 Daniel Gilchrist, Lorelle Meidt, Han Nguyen

BIO 199 STUDENTS WHO RECEIVED EXCELLENCE IN RESEARCH AWARDS: PAPERS PUBLISHED ONLINE

1. Ung, T. and Calof, A.L. (2009) FGF8 Regulates the Development of GnRH+ Neurons. Journal of Undergraduate Research, Academic Year 2008-2009; online publication only (https://www.bio.uci.edu/undergraduates/research/excellence-in-research/ ).

2. Wu, J. and Calof, A.L. (2010) Creating a Gdf11 Conditional Knockout Mouse in the Rx-domain. Journal of Undergraduate Research, Academic Year 2009-2010 ; online publication only (https://www.bio.uci.edu/undergraduates/research/excellence-in-research/ ). 3. Schostak, T. and Calof, A.L. (2011) Establishing Conditional Nipbl Mouse Lines to Model CdLS. Journal of Undergraduate Research, Academic Year 2010-2011 ; online publication only (https://www.bio.uci.edu/undergraduates/research/excellence-in-research/ ). 4. Yaramanoglu, C. and Calof, A.L. (2011) Characterization of Mouse Embryonic Stem Cell Lines with New FLEX Technology in the Nipbl Locus. Journal of Undergraduate Research, Academic Year 2010-2011; online publication only (https://www.bio.uci.edu/undergraduates/research/excellence-in-research/ ). 5. Yazdi, M. and Calof, A.L. (2012) Downregulation of Nipbl in the cardiac primordium gives rise to heart defects in Nipbl conditional mutant mice. Journal of Undergraduate Research, Academic Year 2011-2012; online publication only in (https://www.bio.uci.edu/undergraduates/research/excellence-in-research/ ). Also published online and hardcover in The UCI Undergraduate Research Journal, Volume XV (2012), pp. 55- 61.

6. Mohan, A. and Calof, A.L. (2014) The Pre-Botzinger Complex: Breathing Life into Cornelia de Lange Syndrome. Journal of Undergraduate Research, Academic Year 2013-2014; online publication only (https://www.bio.uci.edu/undergraduates/research/excellence-in-research/ ) . 7. Sharma, J. and Calof, A.L. (2014) Exploring the Origins of Neurological Deficits in Cornelia de Lange Syndrome Using Mice in which the Central Nervous System is NIPBL-Deficient. Journal of Undergraduate Research, Academic Year 2013-2014; online publication only (https://www.bio.uci.edu/undergraduates/research/excellence-in-research/ ) . 8. Villagomez, L. and Calof, A.L. (2014) Curing Retinal Dystrophy by Rescuing Stem Cells in a Mouse Models of Microphthalmia. Journal of Undergraduate Research, Academic Year 2013- 2014; online publication only (https://www.bio.uci.edu/undergraduates/research/excellence-in- research/ ) .

9. Chang, H. and Calof, A.L. (2017) The Vsx2-/-;Gdf11-/- Double Mutant Model of Retinal Dystrophy Rescue. Journal of Undergraduate Research, Academic Year 2016-2017; online 18

publication only (https://www.bio.uci.edu/undergraduates/research/excellence-in-research/ ) . 10. Ha, J. and Calof, A.L. (2017) Assessing Phenotypic Differences in Mice that are Nipbl Deficient in the Central Nervous System, A Model for Neurological Deficits in Cornelia De Lange Syndrome. Journal of Undergraduate Research, Academic Year 2016-2017; online publication only (https://www.bio.uci.edu/undergraduates/research/excellence-in-research/ ) .

11. Varghese, R. and Calof, A.L. (2017) Developmental Abnormalities of the Kidney in Nipbl- Deficient Mice. Journal of Undergraduate Research, Academic Year 2016-2017; online publication only (https://www.bio.uci.edu/undergraduates/research/excellence-in-research/ ).

BIO 199 STUDENTS WHO RECEIVED EXCELLENCE IN UROP and SURP AWARDS:

2011-2012 Mona Yazdi (UROP award) Summer 2013 Abhinav Mohan (SURP award) 2013-2014 Louise Villagomez (UROP award) 2015-2016 Henry Chang (UROP award) 2016-2017 Henry Chang (Second UROP award) Summer 2017 Daniel Gilchrist (SURP award) 2017-2018 Hema Monica Sharda (UROP award)

BIO 199 STUDENTS WHO RECEIVED OTHER AWARDS: Rosaysela Santos Hispanic Scholarship (2002-2003)- $2,500, The Hispanic Scholarship Fund 1st Place Developmental Biology Poster (2004)- Funded to attend and present research at the 2005 Society for Developmental Biology 64th Annual Meeting, MARC-FASEB Program & SDB

Teresa Ung UCI Chancellor’s Awards for Excellence in Undergraduate Research in Medicine 2009 Jocelyn Ha Edward Mittelman Memorial Fund Scholarship, Ayala School of Biological Sciences, UCI 2017

Diversity students mentored in research in the UCI Minority Undergraduate Biomedical Research Support Program (MBRS) and BRIDGES to the Baccalaureate Program Sylvia Jaramillo, 2000 (was also a Bio199 student 2000-2003) Rosaysela Santos, 2002-2005 (**was also a Bio199 student 2002-2004) Aida Brush, 2004 (**was also a Bio199 student in 2004) Annie Espinosa, 2004-2005 (**was also a Bio199 student 2004-2005) Stephen Melgarejo 2005-2006 (**was also a Bio199 student 2005-2006) 19

Devin Arias, 2005-2006 (**was also a Bio199 student 2005-2006) Kimberly Frutoz, 2008

Diversity students mentored in research in the UCI PREP Post-Baccalaureate, Pre-Ph.D. Program 2005-2006 Rafael Gomez-Amaro 2005-2006 Rosaysela Santos 2006-2007 Celina Mojica 2009-2010 Nilsa Mendez

2011-2012 Research supervisor for Salvador Deniz, supported by the CAMP (California Alliance for Minority Participation) program (**was also a Bio199 student 20112012) Summer 2014 Summer research supervisor for Ivory Paulk# (undergraduate from University of Central Florida) in the SURF (Summer Undergraduate Research Fellowship) program, which supports diversity students that UCI Graduate Division seeks to recruit to its Ph.D. programs Summer 2016 Summer research supervision of Daniel Gilchrist in the MCBU (Mathematical and Computational Biology for Undergraduates) program, the goal of which is to prepare undergraduates in interdisciplinary research for future careers in systems biology.

GRADUATE AND POSTBACCALAUREATE STUDENTS SUPERVISED, UCI (# indicates diversity student)

1995 Jianyong Shou 1996 Jianyong Shou, Minako Hashimoto, Mark Cirkot, Steven H.S. Lin 1997 Jianyong Shou, Cameron Hoover, Anna DeHaas-Johnson 1998 Jianyong Shou, Song Zhou, Fumio Ogoshi 1999 Annie Lee, Candice Crocker, Jianyong Shou [Jianyong Shou, Ph.D. September, 1999 (currently Senior Research Director, Biology, Lily China R&D Center, Shanghai, China)] 2000 Candice Crocker 2001 Candice Crocker, Joon Kim 2002 Candice Crocker, Joon Kim, Zhiping Wang, Michael Chou 2003 Candice Crocker, Joon Kim, Ying Wang, Spencer Currle 2004 Candice Crocker, Joon Kim, Erik Pendleton 2005 Candice Crocker, Joon Kim, Erik Pendleton, Matt Groza, Jessica Wu, Lulu Chen, Rafael Gomez-Amaro#, Rosaysela Santos# 2006 Candice Crocker, Joon Kim, Erik Pendleton, Kimberly Gokoffski, Piper Hollenbeck, Rafael Gomez-Amaro#, Rosaysela Santos#, Celina Mojica# Eric Pendleton M.S. 2006 (currently Associate Scientist Vividion Therapeutics, San Diego,

California) Joon Kim, Ph.D., 2006 (currently Associate Professor, KAIST, Grad. Sch. of Med. Sci. & Engineering, Daejeon, South Korea 2007 Candice Crocker, Kimberly Gokoffski, Piper Hollenbeck, Rosaysela Santos#, Celina Mojica#, Chris Sontag, Reginald McNulty# Candice Crocker, Ph.D. 2007 (currently Research Associate and Team Leader, Nova Scotia Early Psychosis Program, Dept. of Psychiatry, Dalhousie Univ,, Halifax, Nova Scotia, Canada 2008 Kimberly Gokoffski, Piper Hollenbeck, Rosaysela Santos#, MichaelCarter, Kimberly Frutos# 2009 Kimberly Gokoffski, Piper Hollenbeck, Rosaysela Santos#, Nilsa Mendez#, Jeremy Ovadia 2010 Kimberly Gokoffski, Piper Hollenbeck, Rosaysela Santos#, Edwin Vargas#, Diane Su, Nilsa Mendez# Kimberly K. Gokoffski, Ph.D. 2010, M.D., 2011 (currently Clinical Instructor of Ophthalmology, Roski Eye Institute University of Southern California, Los Angeles, CA 2011 Piper Hollenbeck, Rosaysela Santos#, Alvaro Ponce Real# Piper Hollenbeck, Ph.D. 2011 (currently Scientist – Clinical Genomics, Invitae: A Genetic Information Company, Palo Alto, CA) 2012 Rosaysela Santos#, Carrie Ng, Arjun Nair, Diego Hoyle#, Phoebe Valdes# Rosaysela Santos#, Ph.D. December 2012 (did Postdoc with Calof 2013-2017, currently Academic Administrator, Dept. of Anatomy & Neurobiology, UC Irvine [Gross Anatomy Instructor, Mentor: Dr. Jamie Wickenheiser]) 2013 Phoebe Valdes, Ziwei Zhong 2014 Phoebe Valdes 2015 Phoebe Valdes, William Sherman, Katrina Taylor Evans 2016 Phoebe Valdes (Phoebe Valdes, M.S. in Biological Sciences, 2016; currently Research Associate, Innovio Pharmaceuticals, Research and Development, San Diego, CA 2017 Stephenson Chea, Adam Stabell 2018 Stephenson Chea, Trini Nguyen (First Year Advisor, MCSB graduate Program) 2019 Stephenson Chea, Trini Nguyen (First Year Advisor, MCSB graduate Program)

AWARDS TO MENTORED GRADUATE STUDENTS DURING REVIEW PERIOD Kimberly Kinga Gokoffsk (awarded the Ph.D. in 2010, the M.D. in 2012) Neurosciences Training Grant NIH GM-08620. Support for two years including $2,200 for experimental materials and $500 travel per year. (2005-2007) 2nd place for oral presentation at MSTP retreat 2006 and 2008. $50 student award. Center for Complex Biological Systems project competition winner, University of California, Irvine (2007) $10,000 granted for project “Stochastic model of stem cell aging”, $500 award for student. NINDS/AUPN/ANA Medical Students Mentoring Workshop Scholarship. Expenses paid to participate in workshop: How to Combine Clinical and Research Careers in Neuroscience. (2007) Travel award to attend International Conference in Systems Biology, Goteborg Sweden. 2008 Paul H. Silverman Award for Research in Stem Cells. (2008) 21

Developmental and Systems Biology Training Grant T32 HD060555-01. Awarded position in 2009 but declined. Center for Complex Biological Systems project competition winner, University of California, Irvine (2009) $10,000 granted for project “Stochastic model of the neuronal gene regulatory network in the olfactory epithelium”, $500 award for student. Graduation with Distinction in Research, School of Medicine, University of California, Irvine Graduation with Distinction in Service, School of Medicine, University of California, Irvine

Piper Nicolosi née Hollenbeck (awarded the Ph.D. in 2011) Spatial Dynamics Research Challenge, Center for Complex Biological Systems, $10,000 for project, $500 award for student (2008) Biological Sciences Dean’s Office Travel Award, University of California, Irvine, $200 (2008) International Symposium for Olfaction and Taste Student Travel Award, San Francisco, California, $300 (2008) Predoctoral Training Grant Award, Eunice Kennedy Shriver National Institute of Child Health and Human Development Grant number: 1 T32 HD060555-01 (tuition, fees, and stipend for 1 year, 2009-2010) University of California, Irvine, CA Biological Sciences Dean’s Office Travel Award, University of California, Irvine, $200 (2009) Paul H. Silverman Memorial Fellowship, University of California, Irvine, $250 (2009) Graduate Dean’s Dissertation Fellowship, University of California, Irvine, CA (2010) - $10,000 School of Medicine Travel Award, University of California, Irvine, CA (2011) - $250 UCI-Howard Hughes Medical Institute Graduate Fellow, Professor Training Program University of California, Irvine, CA (2010-2011) - $500

Rosaysela Santos Research Funding (2006)- $4,000, Alliance for Graduate Education and Professoriate (AGEP) Competitive Edge Summer Program University of California, Irvine, National Science Foundation Grant #HRD 0450366 HHMI-UCI Professor Program: In Situ TA training (2007)- Selected to participate in the In Situ Teacher assistant (TA) training program provided by the Howard Hughes Medical Institute funded UCI Professor Program at the University of California, Irvine Research & Educational Fellowship (2008-2009)- $33,015 - University of California Irvine Faculty Mentor Program Fellowship to Promote Diversity Award to attend the Gordon Research Conference: Mechanisms on Epilepsy and Neuronal Synchronization (2008)- $600, GRC Chair’s Fund Award to attend the Cold Spring Harbor Workshop on Autism Spectrum Disorders (2009)- $1000, Alliance for Graduate Education and Professoriate (AGEP), University of California, Irvine Young Investigator Travel Award- XIVth International Symposium on Retinal Degeneration (2010)- $2000, National Eye Institute Award to attend the Society for the Advancement of Chicanos and Native Americans in Science 22

(SACNAS) National Conference and Mexican American Engineers and Scientists (MEAS) Symposium (2010)- Fully Funded, Alliance for Graduate Education and Professoriate (AGEP), University of California, Irvine The St. Jude Children’s Hospital Award for outstanding Research Presentation (2010)- $500, Graduate Student Oral Presentation Award in the field of Biology at the Society for the Advancement of Chicanos and Native Americans in Science (SACNAS) National Conference and Mexican American Engineers and Scientists (MEAS) Symposium, St. Jude Children’s Hospital Nomination to attend the 17th Annual Compact for Faculty Diversity (2010)- Alliance for Graduate Education and Professoriate (AGEP), University of California, Irvine Award to attend the 17th Annual Compact for Faculty Diversity, Tampa, FL (2010)- Fully Funded, By invitation only; Southern Regional Education Board (SREB)-State Doctoral Scholars Program. Research & Educational Funding (2011-2012)- University of California Irvine, President’s Dissertation Year Fellowship Fifth National CdLS Scientific & Educational Symposium Travel Award (2012)- $400, The Cornelia de Lange Syndrome Foundation, Inc. USA Winner of the 2012 DECADE Logo Design Competition (2012)- $1000 Travel Award, Diverse Educational Community and Doctoral Experience (DECADE), funds to be used towards scientific conference of choice; used to attend the Fifth National CdLS Scientific & Educational Symposium 2012.

Phoebe Valdes (M.S. 2016, in Biological Sciences) HHMI-UCI Professor Program: In Situ TA training (2013)- Selected to participate in the In Situ Teacher assistant (TA) training program provided by the Howard Hughes Medical Institute funded UCI Professor Program at the University of California, Irvine Center for Complex Biological Systems project competition winner, University of California, Irvine (2014-2015) $10,000 granted for project “Prediction of complex developmental phenotypes using multiple data types”, $500 award for student. 2016, GAANN (Graduate Assistance in Areas of National Need) Fellowship, Dept. of Developmental and Cell Biology

POSTDOCTORAL FELLOWS SUPERVISED, UCI 1995 Nobuko Hagiwara, Ph.D. (currently Associate Professor, Division of Cardiovascular Medicine/Dept. of Internal Medicine, University of California, Davis, CA) 1996 Pasquale Iannuzzelli, Ph.D. (currently Pharmaceutical and Strategic Planning Consultant, PGI Medcomm, Philadephia, PA) 1996-97 Quynh Chu-LaGraff, Ph.D. (currently Associate Professor, Department of Biology, Union College, Schenectady, NY) 1997-2003 Richard Murray, Ph.D. (currently Associate Professor, Department of Biology, Hendrix College, Conway, AR) 1999-2003 Alexandre Bonnin, Ph.D. (currently Assistant Professor, Dept. of Cell and

Neurobiology/Zilkha Neurogenetic Institute, Keck School of Medicine, USC, Los Angeles, CA) 2000-2003 Hsiao-Huei (Juli) Wu, Ph.D. (currently R&D Senior Program Administrator, Southern California Clinical and Translational Science Institute, University of Southern California, Los Angeles, CA 2000-2017 Shimako Kawauchi, Ph.D. 2001-2003, Fellow, Human Frontiers Science Program 2003-2005, Postodoctoral Trainee, UCI Carcinogenesis Training Grant 2005-2012, Assistant Project Scientist, UC Irvine 2012-2013, Assistant Professor, University of Trento, Trento, Italy Visiting Researcher, CCBS, UCI 2013- 2017, Associate Project Scientist, CCBS, UC Irvine (as of August 2017, SRA III, Office of Research (TMF), UC Irvine) 2003-2004 Hisaki Hayashi, Ph.D. (currently Instructor, Dept. of Physiology, Aichi Medical University, Nagakute, Japan 2003-2008 Crestina Beites, Ph.D. (currently Associate Professor, School of Midwifery, Sudbury, Ontario, Canada) 2007-2008 Jason Hamilton, Ph.D. (currently Analytical Project Leader (US), Cell and Gene Therapy, Novartis Pharmaceuticals, New York, NY) 2007-2012 Akihiko Muto, Ph.D. (joint with Arthur Lander and Thomas Schilling; currently Assistant Professor, Dept. of Biological Science, Graduate School of Science, Hiroshima University, Hiroshima, Japan) 2012-2013 Xu Wang, Ph.D. (currently Investigator, Dept. of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, China) 2013-2017 Rosaysela Santos, Ph.D. (Currently Academic Administrator, Dept. of Anatomy & Neurobiology, UC Irvine [Gross Anatomy Instructor, Mentor: Dr. Jamie Wickenheiser]) 2014-2016 Nabil Azhar, Ph.D. (currently Senior Bioinformatics Scientist, Netgnix LLC, Irvine, CA)

BIBLIOGRAPHY (PEER-REVIEWED PAPERS; CATEGORY IN BOLD ITALICS) 1. Calof, A.L., Jones, R.B., and Roberts, W.J. (1981) Sympathetic modulation of mechanoreceptor sensitivity in frog skin. J. Physiol. 310: 481-499. Journal article, peer reviewed

2. Lander, A.D. Tomaselli, K.J., Calof, A.L., and Reichardt, L.F. (1983) Studies on extracellular matrix components that promote neurite outgrowth. C.S.H. Symp. Quant. Biol. 48: 611-623. Journal article, peer reviewed 3. Calof, A.L. and Reichardt, L.F. (1984) Motoneurons purified by cell sorting respond to two distinct activities in myotube conditioned medium. Dev. Biol. 106: 194-210. Journal article, peer reviewed

4. Calof, A.L. and Reichardt, L.F. (1985) Response of purified chick motoneurons to myotube conditioned medium: Laminin is essential for substratum-binding, neurite outgrowth- promoting activity. Neuroscience Letters 59: 183-189. Journal article, peer reviewed

5. Calof, A.L. and Chikaraishi, D.M. (1989) Analysis of neurogenesis in a mammalian neuroepithelium: Proliferation and differentiation of an olfactory neuron precursor in vitro. Neuron 3: 115-127. Journal article, peer reviewed

6. Calof, A.L. (1991) Immortalized neurons derived from tumors in transgenic mice. Trends in Neurosciences 14: 1-3. Journal article, peer reviewed [invited review]

7. Calof, A.L. and Lander, A.D. (1991) Relationship between neuronal migration and cell-substratum adhesion: laminin and merosin promote olfactory neuronal migration but are anti-adhesive. J. Cell Biol. 115: 779-794. Journal article, peer reviewed

8. Calof, A.L., Lander, A.D., and Chikaraishi, D.M. (1991) Regulation of neurogenesis and neuronal differentiation in primary and immortalized cells from mouse olfactory epithelium. In: Regeneration of Vertebrate Sensory Receptor Cells. Wiley, Chichester (Ciba Foundation Symposium 160), pp. 249-276. Conference Proceedings, peer-reviewed [book chapter]

9. Calof, A.L. (1992) Sex, nose and genotype. Current Biology 2: 103-105. Journal article, peer reviewed [invited review] 10. Trevino, C., Calof, A.L., and Muneoka, K. (1992) Position specific growth regulation of 3T3 cells in vivo. Dev. Biol. 150: 72-81. Journal article, peer reviewed

11. Calof AL. (1993) Immortalizing neural cells -- Overview. NeuroProtocols 3:175. Journal article, peer reviewed [overview article for special edition, of which I was Editor] 12. Calof, A.L. and Guevara, J. L. (1993) Cell lines derived from retrovirus-mediated oncogene transduction into olfactory epithelium cultures. NeuroProtocols 3: 222-231. Journal article, peer reviewed 13. Lander, A.D. and Calof, A.L. (1993) Extracellular matrix in the developing nervous system. In: Molecular Genetics of Nervous System Tumors. (A.J. Levine and H.H. Schmidek, Eds.) Wiley, New York, pp. 341-355. Book chapter, peer reviewed

14. Calof, A.L., Campanero, M.R., Yurchenco, P.D., and Lander, A.D. (1994) Domain-specific activation of the neuronal migration and neurite outgrowth activities of laminin. Neuron, 13: 117-130. Journal article, peer reviewed

15. DeHamer, M., Guevara, J., Hannon, K., Olwin, B., and Calof, A.L. (1994) Genesis of olfactory receptor neurons: Regulation of progenitor cell divisions by fibroblast growth factors. Neuron 13: 1083-1097. Journal article, peer reviewed

16. Calof, A.L., Adusumalli, M., DeHamer, M., Guevara, J.L., Mumm, J.S., Whitehead, S., and Lander, A.D. (1994) Generation, differentiation, and maturation of olfactory receptor neurons in vitro. In: Olfaction and Taste XI (K. Kurihara, N. Suzuki, H. Ogawa, eds.) Tokyo, Springer-Verlag, pp. 36-40. Conference Proceedings, peer-reviewed [book chapter]

17. Calof, A.L. (1995) Intrinsic and extrinsic factors regulating vertebrate neurogenesis. Current Opinion in Neurobiology 5: 19-27. Journal article, peer reviewed

18. Gordon, M.K., Mumm, J.S., Davis, R.A., Holcomb, J.D. and Calof, A.L. (1995) Dynamics of MASH-1 expression in vitro and in vivo suggest a non-stem cell site of MASH-1 action in the olfactory receptor neuron lineage. Mol. Cell. Neurosci. 6: 363-379. Journal article, peer reviewed 19. Holcomb, J.D., Mumm, J.S. and Calof, A.L. (1995) Apoptosis in the neuronal lineage of the mammalian olfactory epithelium: Regulation in vivo and in vitro. Devel. Biol. 172: 307-323. Journal article, peer reviewed 20. Calof, A.L., Guevara, J.G., and DeHamer, M.K. (1995) Mouse olfactory epithelium. In: Primary Neuronal Cell Culture: A Practical Approach. (J. Cohen and G. Wilken, eds.) Oxford, IRL Press, pp. 205-220. Book chapter, peer reviewed 21. Calof, A.L., Hagiwara, N., Holcomb, J.D., Mumm, J.S., and Shou, J. (1996) Neurogenesis and cell death in the olfactory epithelium. J. Neurobiol. 30: 67-81. Journal article, peer reviewed

22. Holcomb, J.D., Graham, S., and Calof, A.L. (1996) Neuronal homeostasis in mammalian olfactory epithelium: A review. Am. J. Rhinol. 10: 125-134. Journal article, peer reviewed

23. Mumm, J.S., Shou, J., and Calof, A.L. (1996) Colony-forming progenitors from mouse olfactory epithelium: Evidence for feedback regulation of neuron production. Proc. Natl. Acad. Sci. USA 93: 11167-11172. Journal article, peer reviewed

24. Calof, A.L., Holcomb, J.D., Mumm, J.S., Hagiwara, N., Tran, P., Smith, K.M., and Shelton, D.L. (1996) Factors affecting neuronal birth and death in mammalian olfactory epithelium. In: Growth Factors as Drugs for Neurological and Sensory Disorders (G.R. Bock and J.A. Goode, eds.) Wiley, Chichester (Ciba Foundation Symposium 196), pp. 188-210. Conference Proceedings, peer-reviewed [book chapter] 25. Calof, A.L., Mumm, J.S., Rim, P.C, and Shou. J. (1998) The neuronal stem cell of the olfactory epithelium. J. Neurobiol. 36: 190-205. Journal article, peer reviewed

26. Calof, A.L., Rim, P.C, Askins, K.J., Mumm, J.S., Gordon, M.K., Iannuzzelli, P., and Shou, J. (1998) Factors regulating neurogenesis and programmed cell death in mouse olfactory epithelium. In: Olfaction and Taste XII (C. Greer and C. Murphy, eds.) New York, Annals of the New York Academy of Sciences 855, pp. 226-229. Conference Proceedings, peer- reviewed [book chapter] 27. Calof, A.L. (1998) Overcommitment. Current Biology 8: R107. Journal article, other (think piece) 28. Shou, J., Rim, P.C., and Calof, A.L. (1999) BMPs inhibit neurogenesis by a mechanism involving degradation of a transcription factor. Nature Neuroscience 2: 339-345. Journal article, peer reviewed . ** Reviewed in News and Views by G. Fishell, 1999, “BMPs: Time to Murder and Create”, Nature Neuroscience 2, 301 - 303 (1999) doi:10.1038/7208.

29. Murray, R.C. and Calof, A.L. (1999) Neuronal regeneration: Lessons from the olfactory system. Seminars in Cell & Developmental Biology 10: 421-431. Journal article, peer reviewed 26

30. Calof, A.L., Mumm, J.S., Rim, P. and Shou, J. (1999) In vitro analysis of neuronal progenitor cells from mouse olfactory epithelium. In: The Neuron in Tissue Culture (L. Haynes, Ed.) Wiley, Chichester,pp. 23-44. Conference Proceedings, peer-reviewed [book chapter]

31. Calof, A.L. (1999) Grant-writing amnesia. Current Biology 9: R869. Journal article, other (think piece) 32. Murray, R.C., Tapscott, S.J., Petersen, J.M, Calof, A.L., and McCormick, M.B. (2000). A fragment of the neurogenin1 gene confers regulated expression of a reporter gene in vitro and in vivo. Developmental Dynamics 218: 189-194. Journal article, peer reviewed

33. Shou, J., Murray, R. C., Rim, P.C., Kim, Y.C., and Calof, A.L. (2000) Opposing effects of bone morphogenetic proteins on neuron production and survival in the olfactory receptor neuron lineage. Development 127: 5403-5413. Journal article, peer reviewed

34. Calof, A.L. (2000) Long odds. Current Biology 10: R539. Journal article, other (think piece) 35. Calof, A.L., Bonnin, A., Crocker, C., Kawauchi, S., Murray, R., Shou, J., and Wu, H.-H. (2002) Progenitor cells of the olfactory receptor neuron lineage. Microsc. Res. Tech. 58: 176-188. Journal article, peer reviewed 36. Murray, R.C., Navi, D., Fesenko, J., Lander, A.D., and Calof, A.L. (2003) Widespread defects in the developing mammalian olfactory system caused by loss of Mash1 function. The Journal of Neuroscience 23: 1769-1780. Journal article, peer reviewed

37. Wu, H. -H., Ivkovic, S., Murray, R. C., Jaramillo, S., Lyons, K. M., Johnson, J. E., and Calof, A. L. (2003). Autoregulation of neurogenesis by GDF11. Neuron 37: 197-207. Journal article, peer reviewed ** Reviewed in News & Views in Nature Medicine (N.B. Hastings and E. Gould, “Neurons Inhibit Neurogenesis”, 2003, vol. 9, pp. 264-266) and Developmental Cell (L.W. Garner, J. Nove, and V Rosen, “Return of the Chalones”, 2003, vol. 4, pp. 149-151). 38. Krantz, I.D., McCallum, J., DeScipio, C., Kaur, M., Gillis, L., Yaeger, D., Jukofsky, L., Wasserman, N., Bottani, A., Morris, C.A., Nowaczyk, N., Toriello, H., Bamshad, M.J., Carey, J.C., Rappaport, E., Kawauchi, S., Lander, A.D., Calof, A.L., Ki, H.-H., Devoto, M., and Jackson, L.G. (2004) Cornelia de Lange Syndrome is caused by mutations in NIPBL, the human homolog of the Drosophila Nipped-B gene. Nature Genetics 36: 631-635. Journal article, peer reviewed ** Reviewed in “ ‘Nipped’ gene holds key to birth defect”, www.excellence-earlychildhood.ca/documents/Page9Vol4No2Oct05ANG.pdf

39. Kawauchi, S., Beites, C., Crocker, C., Wu, H.-H., Bonnin, A., Murray, R., and Calof, A.L. (2004) Molecular regulation of neurogenesis in the olfactory receptor neuron lineage. Developmental Neuroscience 26: 166-180. Journal article, peer reviewed

40. Beites, C., Kawauchi, S., Crocker, C.E., and Calof, A.L. (2005) Identification and molecular regulation of neural stem cells in the olfactory epithelium. Experimental Cell Research 306: 309-316. Journal article, peer reviewed

41. Kim, J., Wu, H.-H., Lander, A.D., Lyons, K.M., Matzuk, M. M., and Calof, A.L. (2005). GDF11 controls the timing of progenitor cell competence in developing retina. Science 308: 1927-1930. Journal article, peer reviewed ** Reported/reviewed in Science (This Week in Science, “Calling New Neurons” Vol. 308, p.1838); the Orange County Register (June 24, 2005); the Santa Barbara 27

News-Press (June 25, 2005); Obesity, Fitness, and Wellness Week (July 16, 2005), and online at: http://today.uci.edu/news/release_detail.asp?key=1344); http://www.blindness.org/research.asp?id=253; Sci. STKE, Vol. 2005, Issue 290, pp. tw236, 28 June 2005 [DOI: 10.1126/stke.2902005tw236]

42. Kawauchi S., Shou, J., Santos R., Hebert J., McConnell S.K., Mason, I., and Calof, A.L. (2005) Fgf8 expression defines a morphogenetic center required for olfactory neurogenesis and nasal cavity development in the mouse. Development, 132: 5211-5223. Journal article, peer reviewed ** Reviewed in Development (J. Bradbury, “Fgf8 noses into olfactory development”; Development 2005 132: 2303).

43. Ogata, S., Kawauchi, S. Calof, A., and Cho, K.W.Y. (2007) Plastic embedding and sectioning of Xenopus laevis embryos. Journal of Visualized Experiments, http://www.jove.com/index/Details.stp?ID=188. Journal article, peer reviewed 44. Hamilton, JA, Beites, CL, Gokoffski, KK, Hollenbeck PLW, Kawauchi, S, Santos R, Bonnin A, Wu HH, Kim J, & Calof, A.L. (2008) Negative regulation of endogenous stem cells in sensory neuroepithelia: implications for neurotherapeutics. Stem Cell Research and Therapeutics, Y. Shi, D. Clegg (Eds.) Springer, pp. 45-68. Book chapter, invited, peer reviewed

45. Lo, W-C, Chou, C-S, Gokoffski, KK, Wan, FYM, Lander, AD, Calof, AL, and Nie, Q (2009) Feedback regulation in multistage cell lineages. Mathematical Biosciences and Engineering, 6(1): 59-82. Journal article, peer reviewed

46. Lander, A.D., Gokoffski, K.K., Wan, F., Nie, Q., and Calof, A.L. (2009) Cell Lineages and the logic of proliferative control. PLoS Biology 7(1): e1000015. doi:10.1371/journal.pbio.1000015. Journal article, peer reviewed ** Reviewed in G.T. Reeves & S.E. Fraser (2009) “Biological Systems from an Engineer’s Point of View”, PLoS Biology Primer, PLoS Biol 7(1): e1000021. https://doi.org/10.1371/journal.pbio.1000021

47. Kawauchi, S., Kim, J., Santos, R., Wu, H. H., Lander, A. D. and Calof, A. L. (2009). Foxg1 promotes olfactory neurogenesis by antagonizing Gdf11. Development 136, 1453-64. Journal article, peer reviewed 48. Beites, CL, Hollenbeck, PLW, Kim, J, Lovell-Badge, R, Lander, AD, and Calof, AL (2009) Follistatin modulates a BMP autoregulatory loop to control size and patterning of sensory domains in the developing tongue. Development, 136: 2187-2197. Journal article, peer reviewed

49. Kawauchi, S, Calof, AL**, Santos, R., Lopez-Burks, ME, Young, CM, Hoang, MP, Chua, A., Lao, T., Lechner, MS, Daniel, JA, Nussenzweig, A., Kitzes, L., Yokomori, K., Hallgrimsson, B. and Lander, A.D. (2009) Multiple organ system defects and transcriptional dysregulation in a mouse model of Cornelia de Lange Syndrome. PLoS Genetics 5(9): e1000650. Doi:10.1371/journal.pgen.1000650. (** = Co-first author as well as corresponding author) Journal article, peer reviewed 50. Barlow, LA and Calof, AL (2009) Stem cells in sensory epithelium development and regeneration. Ann. NY Acad. Sci., 1170: 5-6. Conference Proceedings, peer-reviewed [journal article]

51. Kawauchi, S, Santos, R, Kim, J, Hollenbeck, PLW, Murray, RC, and Calof, AL (2009) The role of Foxg1 in the development of neural stem cells of the olfactory epithelium. Ann. NY Acad. Sci., 1170: 21-27. Conference Proceedings, peer-reviewed [journal article]

52. Beites, C.L., Kawauchi, S., & Calof, A.L. (2009) Olfactory neuron patterning and specification. In: The New Encyclopedia of Neuroscience, Vol. 7 (L. Zipusky, Ed.) Elsevier, Oxford, pp. 145- 156. Book chapter, invited, peer reviewed.

53. Beites, C.L., Kawauchi, S., & Calof, A.L. (2009) Olfactory neuron patterning and specification. In: Developmental Neurobiology (G. Lemke, Ed.) Elsevier, Oxford, pp. 226-237. Book chapter, invited, peer reviewed 54. Chou, C.-S., Lo, W.-C., Gokoffski, K.K., Zhang, Y.-T., Wan, F.Y.M., Lander, A.D., Calof, A.L., and Nie, Q. (2010) Spatial dynamics of multistage cell lineages in tissue stratification. Biophys. J. 99 (10): 3145-3154. Journal article, peer revieweds

55. Gokoffski, K.K., Kawauchi, S., H.-H. Wu, R. Santos, P.W.L. Hollenbeck, A.D. Lander and A.L. Calof (2010) "Feedback regulation of neurogenesis in the mammalian olfactory epithelium: New insights from genetics and systems biology." In: Neurobiology of Olfaction, (A. Menini, Ed.) CRC Press, pp. 241-265. PMID:21882434. Book chapters, invited, peer reviewed 56. Chien R, Zeng W, Kawauchi S, Santos R, Gregson HC, Schmiesing JA, Newkirk D, Kong X, Ball AR J, Calof AL, Lander AD, Groudine M & Yokomori K (2011) Cohesin mediates chromatin interactions that regulate mammalian beta-globin expression. J. Biol. Chem. 286:DOI 10.1074. Journal article, peer reviewed

57. Gokoffski K, Wu H-H, Beites CL, Kim J, Kim E, Matzuk MM, Johnson JE, Lander AD & Calof AL (2011) Activin and GDF11 collaborate in feedback control of neuroepithelial stem cell proliferation and fate. Development, 138: pp. 4131-4142. Journal article, peer reviewed

58. Muto A, Calof AL, Lander AD, and Schilling, T.F. (2011) Multifactoral origins of heart and gut defects in nipbl-deficient zebrafish, a model of Cornelia de Lange Syndrome. PLoS Biology Oct;9(10):e1001181. Epub 2011 Oct 25. (comment: PLoS Biol. 2011 Oct;9(10):e1001180) PMCID: PMC3201921. Journal article, peer reviewed ** Reviewed in J. Weaver (2011) “Genetic Origins of Birth Defects Revealed by New Animal Model”, PLoS Biology, (http://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1001180).

59. Santos R, Wu H-H, Hamilton J, Pinter R, Hindges R & Calof, AL (2012) Restoration of retinal development in Vsx2 deficient mice by reduction of Gdf11 activity. Adv. Exp. Med. Biol. 723: 671-677. PMID 22183392 Journal article, peer reviewed

60. Lander AD, Kimble J, Clevers H, Fuchs E, Montarras D, Buckingham M, Calof AL, Trumpp A, and Oskarsson T (2012) What does the concept of the stem cell niche really mean today? BMC Biology, 10, 19: pp 1-15; doi: 10.1186/1741-7007-10-19. Journal article, other [review and opinion pieces written by 9 authors; the article was published as a special edition of BMC Biology] 61. Kawauchi S, Santos R, Kim J, Wu, HH, Gokoffski KK, Lander AD and Calof AL (2012) Genetic interactions of cell intrinsic factors and secreted signals regulate neurogenesis in olfactory

epithelium. Journal for the Japanese Association for Study of Taste and Smell 19(1): 49-55. (in Japanese) Conference Proceedings, peer-reviewed [journal article]

62. S. Remeseiro, A. Cuadrado, S. Kawauchi, A.L. Calof, A.D. Lander and A. Losada (2013) Reduction of Nipbl impairs cohesin loading locally and affects transcription but not cohesion- dependent functions in a mouse model of Cornelia de Lange Syndrome. Biochim Biophys Acta 1832, 2097-102. doi: 10.1016/j.bbadis.2013.07.020. PMCID: PMC3825806. Journal article, peer reviewed 63. Visnes, T., F. Giordano, A. Kuznetsova, JA Suja, A.D. Lander, A.L. Calof and L. Strom (2013) Localization of the SMC-loading complex Nipbl/Mau2 during mammalian meiotic prophase I. Chromosoma, 123, 239-252. PMID: 24287868; PMCID: PMC4031387. Journal article, peer reviewed 64. Muto, A., S. Ikeda, M.E. Lopez-Burks, Y Kikuchi, A.L. Calof*, A.D. Lander and T.F. Schilling (2014) Nipbl and Mediator Cooperatively Regulate Gene Expression to Control Limb Development. PLoS Genetics, 10(9):e1004671. doi: 10.1371/journal.pgen.1004671. PMID 25255084. PMCID: PMC4177752. * corresponding author Journal article, peer reviewed

65. Chiang M, Hallman S, Cinquin A, de Mochel NR, Paz A, Kawauchi S, Calof AL, Cho KW, Fowlkes CC, Cinquin O. (2015) Analysis of in vivo single cell behavior by high throughput, human-in-the-loop segmentation of three-dimensional images. BMC Bioinformatics 16:397. https://doi.org/10.1186/s12859-015-0814-7 PMID: 26607933, PMCID: PMC4659165. Journal article, peer reviewed 66. Kunche, S., H. Yan, A.L. Calof*, J.S. Lowengrub and A.D. Lander (2016) Feedback, lineages and self-organizing morphogenesis. PLoS Computational Biol, 12, e1004814. doi:10.1371/journal.pcbi.1004814. Journal article, peer reviewed * corresponding author ** Cited in PLoS Collections, Editor’s Picks in Stem Cell Research (http://collections.plos.org/stem- cell-research).

67. Kawauchi, S., R. Santos, A. Muto, M.E. Lopez-Burks, T.F. Schilling, A.D. Lander and A.L. Calof (2016) Using Mouse and Zebrafish Models to Understand the Etiology of Developmental Defects in Cornelia de Lange Syndrome. Am J Med Genet C Semin Med Genet. 172(2):138-45. doi: 10.1002/ajmg.c.31484. PMID: 27120001. Journal article, peer reviewed

68. Lopez-Burks, M.E., R. Santos, S. Kawauchi, A.L. Calof and A.D. Lander (2016) Genetic enhancement of limb defects in a mouse model of Cornelia de Lange Syndrome. Am J Med Genet C Semin Med Genet. 2016 Jun;172(2):146-54. doi: 10.1002/ajmg.c.31491. PMID: 27120109. Journal article, peer reviewed 69. Santos R., S. Kawauchi, R.E. Jacobs, M.E. Lopez-Burks, H. Choi, J. Wikenheiser, B. Hallgrimsson, H.A. Jamniczky, S.E. Fraser, A.D. Lander, and A.L. Calof* (2016) Conditional creation and rescue of Nipbl-deficiency in mice reveals multiple determinants of risk for congenital heart defects. PLOS Biology, 14, e2000197. doi: 10.1371/journal.pbio.2000197. PMID: 27606604. Journal article, peer reviewed * corresponding author ** Reviewed in: PLoS Biology Primer by B. Gelb (2016)“The Hole and the Whole: Lessons from Manipulation of Nipbl Deficiency” 2016 PLoS Biology, doi: 10.1371/journal.pbio.2000494 Also: Arney K (2016) “Squeezing the Heart” In: Biomedical Picture of the Day (BPoD) http://bpod.mrc.ac.uk/archive/2016/10/13 30

Press releases/wire service pickups: http://www.som.uci.edu/news_releases/risk-factors-congenital-heart-defects-may-lie-inside- outside-heart.asp http://www.eurekalert.org/pub_releases/2016-09/uoc--rff090716.php http://www.newswise.com/articles/risk-factors-for-congenital-heart-defects-may-lie-both- inside-and-outside-the-heart https://www.sciencedaily.com/releases/2016/09/160908150914.htm 70. Newkirk, D., Y.-Y. Chen, R. Chien, W. Zeng, J. Biesinger, E. Flowers, S. Kawauchi, R. Santos, A.L. Calof, A.D. Lander, X. Xie, K. Yokomori (2017) The effect of Nipped-B-like (Nipbl) haploinsufficiency on genome-wide cohesin binding and target gene expression: modeling Cornelia de Lange Syndrome. Clinical Epigenetics, 2017 Aug 25; 9:89. doi: 10.1186/s13148-017- 0391-x. eCollection 2017. Journal article, peer reviewed

71. Crestina L. Beites, Shimako Kawauchi, Rosaysela Santos, Kimberly K. Gokoffski, and Anne L. Calof (2017) Patterning, cell specification and feedback in the olfactory epithelium. In: Reference Module in Neuroscience and Biobehavioral Psychology, Elsevier, 2017, pp. 1-14; ISBN 978-0-12-809324-5, https://doi.org/10.1016/B978-0-12-809324-5.02621-3.Book chapter, invited, peer reviewed 72. Calof AL, Santos R, Groves L, Oliver C, and Lander AD. “Cornelia de Lange Syndrome: Insights into Neural Development from Clinical Studies and Animal Models.” In: Comprehensive Developmental Neurosciences Series, 2nd Edition, Neural Developmental Disorders Section (Anthony Winshaw-Boris, Editor), Elsevier Press. Book chapter, invited, peer-reviewed, in preparation. 73. Calof AL, Maclean A, Kopez-Burks M, Villagomez L, Bui B, Santos R, and Lander AD. Transcriptomic Approach to Identify Therapies and Therapeutic Targets for Cornelia de Lange Syndrome. In preparation.

BIBLIOGRAPHY (OTHER PUBLICATIONS) 1. Calof, A.L. (1993) Review of Cell Biology of Olfaction (by A. Farbman, Cambridge University Press, 1992). Trends in Neuroscience 16: 489-490. Review of a book, commissioned by the editors of Trends in Neuroscience, which is a peer-reviewed journal. 2. Daniel A. Newkirk, Yen-Yun Chen, Richard Chien,Weihua Zeng, Jacob Biesinger, Ebony Flowers, Shimako Kawauchi, Rosaysela Santos, Anne L. Calof, Arthur D. Lander, Xiaohui Xie, and Kyoko Yokomori (May 2017) The effect of Nipped-B-like (Nipbl) haploinsufficiency on genome-wide cohesin binding and target gene expression: modeling Cornelia de Lange Syndrome. BioRXiv (Cold Spring Harbor Laboratory), posted online May. 5, 2017; doi: http://dx.doi.org/10.1101/134825. Online archived article. *** Please note that BioRXiv is a widely-used repository/online journal for pre-print data and manuscripts in the field of genomics. Most people post to it and most in the field also cite it in publications.

BIBLIOGRAPHY (ABSTRACTS)

1. Roberts, W.J. and Calof, A.L. (1977) Sympathetic influence on responses of frog cutaneous receptors. Soc. Neurosci. Abstr. 3: 490. 2. Reichardt, L.F., Calof, A.L., Gorin, P.D., Greenspan, R.J., Greif, K.F., Lander, A.D., Matthew, W.D., Shelton, D.L., Tomaselli, K.J., and Winter, J. (1983) Neurotrophic and substrate activated outgrowth factors. (Symposium Lecture) 7th European Neuroscience Congress, Hamburg, West Germany. Neuroscience Letters 0: Suppl. 14, 5302.

3. Calof, A.L. and Reichardt, L.F. (1983) Effects of conditioned media on neurite outgrowth and survival of purified motoneurons. Soc. Neurosci. Abstr. 9:9. 4. Calof, A.L. and Reichardt, L.F. (1984) Purified motoneurons respond to two distinct activities in myotube conditioned medium. Soc. Neurosci Abstr. 10: 370. 5. Lander, A.D., Calof, A.L., Winter, J., Tomaselli, K.J., and Reichardt, L.F. (1985) Role of laminin and laminin-containing complexes in promoting process outgrowth by neurons. Cell Differentiation 16: Suppl. 1725.

6. Studies on neurotrophic and substrate — attached neurite outgrowth factors Reichardt LF, Calof AL, Goren PD, Greenspan RJ, Greif KF, Lander AD, Matthew WD, Shelton DL, Tomaselli K, Winter J. International Journal of Developmental Neuroscience. 1983 December; 1(3):200 7. Calof, A.L. and Jessell, T.M. (1986) Use of a retroviral vector to transfer and express a foreign gene in murine neurons and neural ectoderm. Soc. Neurosci. Abstr. 12: 183. 8. Calof, A.L. and Chikaraishi, D.M. (1988) In vitro analysis of neurogenesis in a mammalian neuroepithelium. Soc. Neurosci. Abstr. 14: 162. 9. Calof, A.L. and Chikaraishi, D.M. (1988) Identification of a proliferating neuronal precursor cell in cultures of a mammalian neuroepithelium. J. Cell Biol. 107: 510a.

10. Calof, A.L., Lander, A.D., and Chikaraishi, D.M. (1989) Regulation of neurogenesis and neuronal differentiation in primary and immortalized cells from mouse olfactory epithelium. J. Cell Biol. 109: 57a. 11. Trevino, C., Calof, A.L., and Muneoka, K. (1990) Position specific growth of 3T3 cells in the developing mouse limb. J. Cellular Biochem. Suppl. 14E: 100. 12. Calof, A.L. and Lander, A.D. (1990) In vitro analysis of neuronal precursor migration from mouse olfactory epithelium: evidence for regulation by extracellular matrix. J. Cell Biol. 111: 491a.

13. Calof, A.L. and Lander, A.D. (1991) Laminin and merosin promote neuronal migration, but are anti-adhesive. Soc. Neurosci. Abstr. 17: 204. 14. Calof, A.L., Yurchenco, P.D., O'Rear, J.J., and Lander, A.D. (1991) Relationship between neuronal migration and cell-substratum adhesion: laminin and merosin promote olfactory neuronal migration but are anti-adhesive. J. Cell Biol. 115: 5a. 15. Lander, A.D., Calof, A.L., Kindt, R.M., and Plantefaber, L.C. (1992) The neuronal response to laminin. Anatomical Record 232: 53A.

16. Lander, A.D., Calof, A.L., Kindt, R.M., and Plantefaber, L.C. (1992) The neuronal response to laminin. Abstracts of the 5th International Congress on Cell Biology, Madrid, Spain: 176. 17. Calof, A.L., Yurchenco, P.D., O'Rear, J.J., and Lander, A.D. (1992) Adhesion, anti-adhesion, and migration of olfactory neurons and neuronal precursors are regulated by distinct molecular domains of laminin. Soc. Neurosci. Abstr. 18: 925.

18. Calof, A.L., Yurchenco, P.D., O'Rear, J.J., and Lander, A.D. (1992) Adhesion, anti-adhesion, and migration of olfactory neurons and neuronal precursors are independently regulated by distinct molecular domains of laminin. Mol. Biol. Cell 3: 74a. 19. Lander, A.D., Campanero, M.R., Yurchenco, P.D., and Calof, A.L. (1993c) Domain-specific regulation of neurite outgrowth- and neuron-migration-promoting activities of laminin. Mol. Biol. Cell 4: 181a. 20. Calof, A.L., Escandon, E., Guevara, J.L., Mumm, J.S., Nikolics, K., and Whitehead, S.J. (1993b) Regulation of olfactory receptor neuron maturation and survival in vitro. Mol. Biol. Cell 4: 371a.

21. Calof, A.L., DeHamer, M., Adusumalli, M., Guevara, J., Lopez, A., and Olwin, B. (1993a) Generation of olfactory receptor neurons in vitro: characterization of neuronal progenitor cells and their mitogens. Soc. Neurosci. Abstr. 19: 1286. 22. Calof, A.L., Adusumalli, M. D., DeHamer, M., Guevara, J.L., Mumm, J.S., and Whitehead, S.J. (1994) Generation, differentiation, and maturation of olfactory receptor neurons in vitro. J. Cellular Biochem. S18B: 171. 23. Holcomb, J.D., Guevara, J.L., Whitehead S. and Calof, A.L. (1994) Apoptosis in cultured, axotomized and regenerating olfactory receptor neurons. Otolaryngology - Head and Neck Surgery 111: P154. 24. Holcomb, J.D., Guevara, J.L., and Calof, A.L. (1994) Dynamics of apoptosis in murine olfactory epithelium following removal of target-derived trophic support: studies in vitro and in vivo. Soc. Neurosci. Abstr. 20: 432.

25. Calof, A.L., Guevara, J.L., Hannon, K., Olwin, B.B. and DeHamer, M.K. (1994) Genesis of olfactory receptor neurons proceeds through distinct progenitor cell stages and is regulated by fibroblast growth factors. Soc. Neurosci. Abstr. 20: 1275. 26. Lander, A.D., Kindt, R.M., Ivins, J.K., Campanero, M.R., Yurchenco, P.D., and Calof, A.L. (1994) Effects of laminins on neurons. Mol. Biol. Cell 5: 7a. 27. Holcomb, J.D. and Calof, A.L. (1995) Dynamics of cell death in murine olfactory epithelium. Abstracts of the Association for Research in Otolaryngology, 18th Annual Meeting: 163.

28. Holcomb, J.D., Graham, S., and Calof, A.L. (1995) Neuronal homeostasis in mammalian olfactory epithelium: a review. Abstracts of the American Rhinologic Society 41st Annual Scientific Meeting: 24. 29. Calof, A.L., Mumm, J.S., Davis, R.A., Holcomb, J.D., and Gordon, M.K. (1995) Dynamics of MASH1 expression in mouse olfactory epithelium suggests a non-stem cell site of MASH1 action. Soc. Neurosci. Abstr. 21: 1512. 33

30. Hagiwara, N., Mumm, J.S., and Calof, A.L. (1995) Characterization and immortalization of olfactory receptor neuron progenitor cells from mouse olfactory epithelium. Soc. Neurosci. Abstr. 21: 287. 31. Holcomb, J.D., Mumm, J.S., and Calof, A.L. (1995) Regulation of apoptosis in the neuronal lineage of mouse olfactory epithelium. Soc. Neurosci. Abstr. 21: 1788.

32. Calof, A.L., Mumm, J.S., Rim, P.C., and Shou, J. (1996) Regulation of neurogenesis by neuronal colony-forming progenitors isolated from mouse olfactory epithelium. Soc. Neuro. Abstr. 22: 285. 33. Shou, J., Mumm, J.S., Rim, P.C., and Calof, A.L. (1996) Differentiated olfactory receptor neurons feed back to inhibit neurogenesis by neuronal colony-forming progenitors isolated from mouse olfactory epithelium. Mol. Biol. Cell 7: 317a. 34. Calof, A.L., Rim, P.C., Askins, K.J., Mumm, J.S., Gordon, M.K., Iannuzzelli, P., and Shou, J. (1997) Factors regulating neurogenesis and programmed cell death in mouse olfactory epithelium. Chemical Senses 22: 654-655.

35. Shou, J., Rim, P.C., and Calof, A.L. (1997) Feedback inhibition of neurogenesis mediated by TGF-beta superfamily members. Soc. Neuro. Abstr. 23: 298. 36. Calof, A.L., Iannuzzelli, P., Rim, P.C., MacArthur, C., Mason, I., and Shou, J. (1997) Potential autocrine role for FGF8 in regulating neurogenesis in mammalian olfactory epithelium. Soc. Neuro. Abstr. 23: 298. 37. Shou, J. Rim, P.C., and Calof, A.L. (1998) Mechanism of negative regulation of olfactory neurogenesis by bone morphogenetic proteins. Soc. Neuro. Abstr. 24: 1526. 38. Murray, R.C., Askins, K.J., Osburn, S.W., and Calof, A.L. (1998) Expression of Noggin in the olfactory epithelium of transgenic mice: an approach to determine the role of BMPs in the regulation of olfactory neurogenesis. Soc. Neuro. Abstr. 24: 1526.

39. Shou, J., Rim, P.C., and Calof, A.L. (1998) Mechanism of inhibitory action by bone morphogenetic proteins on olfactory neurogenesis. Mol. Biol. Cell 9: 313A. 40. Calof, A.L. Murray, R.C., Gopalan, S., Rim, P.C., Mumm, J.S., and Shou, J. (1999) Regulation of neuron number in mammalian olfactory epithelium. Abstracts of the 22nd Midwinter Research Meeting, Assoc. for Research in Otolaryngology: 108.

41. Huang, X.-C., Calof, A.L., and Sumners, C. (1999) Angiotensin II (Ang II) Type 1 (AT1) receptor-mediated neuronal migration in mouse olfactory epithelium explant cultures. The FASEB Journal 13: A139.

42. Murray, R.C., McCormick, M.B., Tapscott, S.J., and Calof, A.L. (1999) Characterization of a neuroD3 upstream fragment that directs regulated expression of a reporter gene in P19 cells and in transgenic mice. Soc. Neuro. Abstr. 25: 248. 43. Shou, J., Murray, R.C., Kim, Y.C., Rim, P.C., and Calof, A.L. (1999) BMP4 exerts concentration-dependent effects on olfactory neurogenesis. Soc. Neuro. Abstr. 25: 248. 44. Calof, A.L., Shou, J., Murray, R.C., Chern, P.L., Crocker, C.E., Pichard, D.S.L., and Bonnin, A.

(2000) Multiple roles of bone morphogenetic proteins in the regulation of neurogenesis. J. Neurochem. 74: S2. 45. Bonnin, A., Chern, P.L., Yi, S.E., Lyons, K.M., and Calof, A.L. (2000) The type I bone morphogenetic protein receptor BMPRIB is required for the antineurogenic effect of BMP7 in the olfactory epithelium. Soc. Neuro. Abstr. 26: 1087.

46. Crocker, C.E., Chern, P.L., Murray, R.C., and Calof, A.L. (2000) Role of bone morphogentic protein 12 (GDF7) in regulating neurogenesis in the mouse olfactory epithelium. Soc. Neuro. Abstr. 26: 1343. 47. Calof, A.L., Bonnin, A., Chern, P.L., Crocker, E.E., Murray, R.C., Shou, J., and Wu, H.H. (2000) Bone morphogenetic protein share multiple roles in the regulation of olfactory neurogenesis. Proceedings of the International Society for Developmental Neurobiology 2000 meeting (Heidelberg, Germany): 109. 48. Calof, A.L., Bonnin, A., Chern, P.L., Crocker, C.E., Murray, R.C., Shou, J., and Wu, H.-H. (2000) Multiple roles for bone morphogenetic proteins in regulating development of a neuronal lineage. Proceedings of the International Congress on Differentiation and Cell Biology 2000, Gold Coast, Australia.

49. Wu, H.-H., Chern, P., Johnson, J.E., and Calof, A.L. (2001) BMP11 - a candidate negative regulator of olfactory neurogenesis. Developmental Biology 235: 47. 50. Bonnin, A., Wu, H.-H., Yi, S.E., Lyons, K.M., and Calof, A.L. (2001) BMPRIB acts as a brake on neurogenesis in the olfactory epithelium. Soc. Neuro. Abstr. 27: 934. 51. Crocker, C.E., Wu, H.-H, Bonnin, A., and Calof, A.L. (2001) Actions of bone morphogenetic protein 12 (GDF7) on neuronal progenitors of mouse olfactory epithelium. Soc. Neuro. Abstr. 27: 934. 52. Murray, R.C. and Calof, A.L. (2001) Altering the level of BMP signaling in the olfactory epithelium of transgenic mice affects the dynamics of neurogenesis. Soc. Neuro. Abstr. 27: 934.

53. Calof, A.L., Bonnin, A., Crocker, C.E., Kawauchi, S., Kim, J., Murray, R.C., Shou, J., and Wu, H.-H. (2001) Bone morphogenetic proteins: multifunctional regulators of neurogenesis. Journal of Rehabilitation Research and Development 38: S12. 54. Jaramillo, S., Wu, H.H., and Calof, A.L. (2001). Neurogenesis in the olfactory epithelium of the mouse embryo. AAAS Annual Meeting and Science Innovation Exposition (San Franscisco, CA) 167: A87. 55. Kawauchi, S. Shou, J., and Calof, A.L. (2002) Requirement for Fgf8 in olfactory neurogenesis. Dev. Biol. 247: 499.

56. Murray, R. C., Lander, A. D., and Calof, A. L. (2002) The Tattler-4 transgenic reporter mouse reveals multiple defects in the primary olfactory pathway of Mash1-deficient mice. Soc. Neuro. Abstr. 28: Program Number 626.9. 57. Jaramillo, S., Wu, H.H., and Calof, A.L. (2002) BMP11, a likely negative regulator of neural proliferation in the olfactory epithelium. AAAS Annual Meeting and Science Innovation 35

Exposition (Boston, MA) 168: A105. 58. Jen, Y.H.L., Musacchio, M., Calof, A.L., and Lander, A.D. (2002) A major determinant of brain weight linked to the Glypican-1 (GPC1) locus of mice. Soc. Neuro. Abstr. 28: Program Number 23.12.

59. Calof, A.L., Bonnin, A., Crocker, C., Jaramillo, S., Kawauchi, S., Kim, J., Ly, A., Murray, R., Santos, R., Silman, E., Wu, H.-H. (2003) Studies of the neuronal stem cell of the olfactory receptor neuron lineage. Abstracts of the 26th Midwinter Research Meeting, Assoc. for Research in Otolaryngology: Abstract Number 1395. 60. Calof, A.L., Wu, H.-H., Kim, J., Jaramillo, S., and Ly, A. (2003) Feedback regulation of neurogenesis. Abstracts of the June, 2003 Juan March Meeting on Developmental Biology (Madrid, Spain), "Developmental Mechansisms in Vertebrate Organogenesis" 61. Wu, H.-H., Kim, J., Ly, A., Ivkovic, S., Lyons, K., Matzuk, M., and Calof, A.L. (2003) Role of TGF-betas in negative autoregulation of neurogensis. Soc. Neuro. Abstr. 29: Program Number 349.1.

62. Murray, R.C., Blitz, I.L., and Calof, A.L. (2003) Altering BMP levels in vivo affects neuron generation and apoptosis in the olfactory epithelium. Soc. Neuro. Abstr. 29: Program Number 349.17. 63. Kawauchi, S., Shou, J., Santos, R., and Calof, A.L. (2003) Role of Fgf8 in regulating olfactory neurogenesis. Soc. Neuro. Abstr. 29: Program Number 349.11. 64. Crocker, C.E. and Calof, A.L. (2003) Actions of GDF7 on neuronal progenitors of mouse olfactory epithelium. Soc. Neuro. Abstr. 29: Program Number 349.2. 65. Kim, J., Wu, H.-H., Ivkovic, S., Lyons, K., and Calof, A.L. (2003) Role of Gdf11 in retinal neurogenesis. Dev. Biol. 259: 476.

66. Santos, R., Kawauchi, S. and Calof, A.L. (2003) Identifying the regulatory role of Fgf8 in mammalian OE neurogenesis. National Conference and K-12 Teacher Workshop 167: 185 67. Calof, A.L., Beites, C., Crocker, C., Hayashi, H., Kim, J., Silman, E., Santos, R., Kawauchi, S. (2004) Common molecular signals regulating progression through the neuronal lineage in olfactory and other sensory epithelia. AChemS 26: ID 118006 (Symposium) 68. Calof, A.L., Kim, J., Silman, E., and Wu, H.-H. (2004) GDF11 and follistatin interact to govern cell number and cell-type determination in developing mouse nervous system. Abstracts of the Santa Cruz Conference on Developmental Biology, Aug. 5-9, 2004, p. 30.

69. Wineland, A., Blitz, I.L., Murray, R.C., and Calof, A.L. (2004) A Transgenic Approach for Determining the Role of Bone Morphogenetic Proteins in the Development of Olfactory Receptor Neurons. Dev. Biol. 271: 606. 70. Kim, J., Wu, H.-H., and Calof, A.L. (2004) Gdf11 and follistatin interact to govern cell number and cell-type determination in developing mouse retina. Soc. Neuro. Abstr. 30: Program Number 150.17.

71. Crocker, C.E. and Calof, A.L. (2004) GDF7 regulates transit amplifying divisions of neuronal progenitor cells in mouse olfactory epithelium. Soc. Neuro. Abstr. 30: Program Number 491.15. 72. Beites, C., Kawauchi, S., and Calof, A.L. (2004) Is the transcription factor Sox2 a stem cell marker in the olfactory epithelium of the mouse? Soc. Neuro. Abstr. 30: Program Number 150.18.

73. Santos, R., Kawauchi, S., and Calof, A.L. (2004) Requirement of Fibroblast Growth Factor 8 During Olfactory Epithelium Neurogenesis. 2004 AAAS Annual Meeting 24:11. 74. Santos, R., Kawauchi, S. and Calof, A.L. (2004) Fibroblast Growth Factor 8: A crucial signaling molecule for olfactory epithelium neurogenesis. Annual Biomedical Research Conference for Minority Students, Dallas, TX, November 10-13, 2004. **First Prize, best Developmental Biology Poster, awarded jointly by FASEB/Society for Developmental Biology. 75. Kawauchi, S., Shou, J., Santos, R., and Calof, A.L. (2005) Fgf8 controls neurogenesis in mouse olfactory epithelum by regulating survival of stem cells. Keystone Meeting on Cancer and Development, Banff, Canada, Program #213.

76. Beites, C., Kawauchi, S., and Calof, A.L. (2005) Does Sox2 expression define a bipotential stem cell in the olfactory epithelium? Proceedings of the 3rd Annual Meeting of the International Society for Stem Cell Research, San Francisco, CA, Control Number 2004-A-1030-ISSCR. 77. Santos, R., Kawauchi, S. and Calof, A.L. (2005) Fibroblast Growth Factor 8: A crucial signaling molecule for olfactory epithelium neurogenesis. Abstracts of the Annual Meeting of the Society for Developmental Biology (Control Number 183). 78. Kim, J., Wu, H.-H., and Calof, A.L. (2005) GDF11 regulates cell number and cell-fate determination in developing mouse retina. Proceedings of the ARVO Western Eye Research Conference, control Number 05-A-13-WERC. 79. Krantz, I.D., McCallum, J., DeScipio, C., Kaur, M., Gillis, L.A., Yaeger, D., Jukofsky, L., Wasserman, N., Bottani, A., Morriss, C.A., Nowaczyk, J.M., Toriello, H., Bamshad, M.J., Carey, J.C., Rappaport, E., Kawauchi, S., Lander, A.D., Calof, A.L., Li, H.-H., Devoto, M., and Jackson, L.G. (2005). Mutations in NIPBL, the human homologue of the Drosophila Nipped-B gene, cause Cornelia de Lange Syndrome. Proceedings of the Greenwood Genetic Center 24: 171-172.

80. Lander, A.D., Kawauchi, S., Lopez-Burks, M., Chua, A., and Calof, A.L. (2005) Towards a mouse model of Cornelia de Lange Syndrome. Proceedings of the Greenwood Genetic Center 24: 172- 173. 81. Melgarejo, S., Kawauchi, S., and Calof, A.L. (2006) Investigating the roles of GDF11 and follistatin in hair cell genesis in the inner ear. 2006 AAAS Ann. Meeting 26: p. 98. 82. Kawauchi, S., Santos, R., Lopez-Burks, M., Chua, A., Kitzes, L., Lander, A.D., and Calof, A.L. (2006) Developmental defects in multiple organ systems in a mouse model of Cornelia de Lange Syndrome. Abstracts of the Annual Meeting of the Society for Developmental Biology, Developmental Biology 352: 66.

83. Santos, R., Kawauchi, S., Lopez-Burks, M., Yong, C.M., Hoang, M.P., Quach, A.S., Halgrimson, B., Kitzes, L.M., Lander, A.D. and Calof, A.L. (2007) Multi-Organ System Defects in a Mouse Model of Cornelia de Lange Syndrome. West Coast Society for Developmental Biology Regional 37

Conference: 20.40. Pacific Grove, CA 84. Lander, A.K., Kawauchi, S., Santos, R., Lopez-Burks, M., Chua, A., Kitzes, L., and Calof, A.L. (2007) Multi-organ system defects in a mouse model of Cornelia de Lange Syndrome. American Journal of Human Genetics, Part A 143A:1299.

85. Lander, A.D., Kawauchi, S., Santos, R., Lopez-Burks, M., Hoffman, T., Young, C.M., Hoang, M.P., Chua, A., Lao, T., Lechner, S., Halgrimson, B., Schilling, T.F. and Calof, A.L. (2007) Vertebrate Animal Models of Nipbl Deficiency. Abstracts of the First International Meeting on Cohesin Biology and the Cohesinopathies. Varenna, Italy. 86. Calof, A.L., Kawauchi, S., Hamilton, J., Santos, R., Lopez-Burks, M., Young, C.M., Hoang, M.P., Chua, A., Kitzes, L.M., A., Halgrimson, B., and Lander, A.D. (2007) Developmental and Neurological Phenotypes of CdLS: Insights from the Nipbl Mutant Mouse. Abstracts of the First International Meeting on Cohesin Biology and the Cohesinopathies. Varenna, Italy.

87. Gokoffski KK, Wu HH, Kim J, Phan D, Beites CL, Matzuk MM, Calof AL. 2007. ActivinβB Regulates Neural Stem and Progenitor Cell Proliferation and Differentiation in Mammalian Olfactory Epithelium. (30.19), Abstracts of the Society for Neuroscience Annual Meeting, San Diego, CA. 88. Kawauchi, S, Young, C, and Calof, AL (2007) Requirement for Fgf8 in the generation of GnRH- expressing hypothalamic neurons during mouse development (562.7). Abstracts of the Society for Neuroscience Annual Meeting, San Diego, CA. 89. Gokoffski KK, Wu HH, Kim J, Phan D, Beites CL, Matzuk MM, Calof AL. 2007 A Tripartite Feedback System Regulates Neural Stem and Progenitor Cell Proliferation and Differentiation in Mammalian Olfactory Epithelium. West Coast Society for Developmental Biology Conference, Pacific Grove, CA.

90. Gokoffski KK, Wu HH, Kim J, Phan D, Beites CL, Matzuk MM, Calof AL. 2007 A Tripartite Feedback System Regulates Neural Stem and Progenitor Cell Proliferation and Differentiation in Mammalian Olfactory Epithelium. Keystone Symposium: Stem Cell Interactions with their Microenvironmental Niche (X1), Keystone, CO: 154, p 66. 91. Lander, AD, Gokoffski, K, and Calof, AL (2007) Feedback control of size, cellular composition, and regneration speed in neural epithelia: Experimental and computational analysis. Abstracts of the 8th International Conference on Systems Biology (ICSB 2007; B04), Long Beach, CA. 92. Yosiphon, G, Gokoffski, KK, Calof, AL, Lander AD, and Mjolsness, E (2007) Dynamical grammar modeling of cellular proliferative dynamics in the olfactory epithelium. Abstracts of the 8th International Conference on Systems Biology (ICSB 2007; E14), Long Beach, CA.

93. Yosiphon G, Gokoffski KK, Calof AL, Lander AD, Mjolsness E. (2007) Stochastic Multiscale Modeling Methods of Stem Cell Niches. Abstracts of the Neural Information Processing Systems Conference: Workshop on Machine Learning in Computational Biology, Whistler, B.C. 94. Yosiphon, G., Gokoffski, K.K., Calof, A.L., Lander, A.D., and Mjolsness, E. (2007) Stochastic Stochastic Multiscale Modeling Methods for Stem Cell Niches. Machine Learning in

Computational Biology workshop (NIPS-MLCB) http://raetschlab.org:10080/nipscompbio/previous/MLCB2007/program/abstracts 95. Hamilton, JA, Kim, J, and Calof, AL (2007) GDF11-FST regulation of retinal neurogenesis in normal and ocular mutant mice. Abstracts of the Pacific Ocular Regenerative Biology Conference X11, p. 19.

96. Kawauchi, S, Santos, S, Lopez-burks, ME, Hoffman, T, Young, CM, Hoang, MP, Chua, A, Lao, T, Lechner, MS, Kitzes, L, Hallgrimsson, B, Schilling, TF, Calof, AL, and Lander, AD (2007) Vertebrate animal models of Cornelia de Lange Syndrome. 5th Structural birth Defects Working Group Meeting, National Institute of Child Health and Human Development, Baltimore, MD. 97. Kawauchi S., Kim J., Santos R., and Calof A.L. (2008) Opposing actions of cell-intrinsic factors and secreted signals regulate neurogenesis in olfactory epithelium. Abstract #611, Proceedings of the 15th International Symposium on Olfaction and Taste. San Francisco, CA. 98. Hollenbeck P.L.W., Beites C.L., Kim J, Lovell-Badge R, Calof A.L. (2008) Follistatin directs patterning and development of Sox2-expressing taste bud progenitors. Abstract #120, Proceedings of the 15th International Symposium on Olfaction and Taste. San Francisco, CA.

99. Kawauchi, S, Santos, R, Lopez-Burks, ME, Hoang, MP, Kitzes, LM, Lao, T, Lechner, MS, Hallgrmsson, B, Daniel, JA, Nussenzweig, A, Lander, AD, and Calof, AL. (2008) Insights into Cornelia de Lange Syndrome from the Nipbl-mutant mouse. Am. J. Med. Genet. Part A, 149A: 1620-1621.

100. Muto, A, Hoffman, T, Kawauchi, S, Schilling, TF, Lander, AD, and Calof, AL (2008) Modeling early developmental defects in Cornelia de Lange Syndrome using the zebrafish. Am. J. Med. Genet. Part A, 149A: 1621. 101. Lander, AD, Kawauchi, S, Santos, R, Lopez-Burks, ME, Hoffman, T, Muto, A, Young, CM, Hoang, MP, Chua, Am Kitzes, LM, Lao, T, Lechner, MS, Hallgrimsson, B, Schilling, TF, and Calof, AL. (2008) Vertebrate animal models of Cornelia de Lange Syndrome. 6th Structural Birth Defects Working Group Meeting, National Institute of Child Health and Human Development, Bethesda, MD.

102. Gokoffski KK, Wu HH, Kim J, Beites CL, Lander AD, Calof AL. 2008. ActivinβB, GDF11 and Follistatin participate in a feedback network that regulates genesis of neurons and glia by olfactory stem cells. Abstracts of the 9th International Conference on Systems Biology (ICSB 2008), Gothenburg Sweden. 103. Calof, AL, Kawauchi S, Santos, R, Lopez-Burks, ME, Hoang, MP, Kitzes, LM, Lao, T, Lechner, M., Halgrimsson, B, Daniel, JA, Nussenzweig, A., Lander, AD. (2009) Insights into Cornelia de Lange Syndrome from the Nipbl-mutant mouse. Abstracts of the Second International Meeting on Cohesin Biology and the Cohesinopathies. Certosa di Pontignano, Siena, Italy (American Journal of Human Genetics). 104. Lander, AD, Muto, A, Hoffman, T, Kawauchi, S, Schilling, TF, and Calof, AL (2009) Modeling early developmental defects in Cornelia de Lange Syndrome using the zebrafish. Abstracts of the Second International Meeting on Cohesin Biology and the Cohesinopathies. Certosa di Pontignano, Siena, Italy (American Journal of Human Genetics, in press).

105. Scott Christley, Piper Hollenbeck, Ching-Shan Chou, Arthur Lander, Anne Calof and Qing Nie. Computational modeling of taste papillae patterning during development. (ICSB 2009): Abstracts of the 10th International Conference on Systems Biology, Stanford, California, August 2009, Poster 1.082. 106. Hollenbeck P.L.W., Beites C.L., Kim J, Lovell-Badge R, Christley S, Nie Q, Lander A.D., Calof A.L. (2009) A Follistatin-BMP7 Feedback Circuit Controls Taste Papillae Development and Patterning in Mouse Tongue. Abstract#497, Proceedings of the Society for Developmental Biology Annual Meeting, San Francisco CA; Developmental Biology 331: A167.

107. Muto A., Schilling T.F., Calof A.L., Lander, A.D. (2010) Regulation of endoderm development by zebrafish Nipbl. Proceedings of the 69th Society for Developmental Biology Annual Meeting, doi:10.1016/j.ydbio.2010.05.433 108. Kawauchi S., Santos R., Lopez-Burks M., Alvarez A., Chua T., Luu R., Schostak T., Lander A.D., Calof A.L. (2010) Dysregulation of gne expression, adipogenesis, and heart development in the Nipbl+/- mouse, a model for Cornelia de Lange Syndrome. Proceedings of the 4th Biennial Symposium of the CdLS Foundation; Am J Med Genet Part A 152A: 2686-2687. 109. Santos, R., Wu, J., Hamilton, J.A., Pinter, R., Hindges, R., and Calof, A.L. (2010). Restoration of retinal development in Vsx2 deficient mice by reduction of Gdf11 activity. XIVth International Symposium on Retinal Degeneration: 23.131. Mont-Tremblant, Quebec, Canada. 110. Santos, R., Wu, J., Hamilton, J.A., Pinter, R., Hindges, R., and Calof, A.L. (2010). Restoration of retinal development in Vsx2 deficient mice by reduction of Gdf11 activity. Society for the Advancement of Chicanos and Native Americans in Science (SACNAS) National Conference and Mexican American Engineers and Scientists (MEAS) Symposium 2010: 12. Anaheim, CA.

111. Kawauchi, S., Santos, R., Lopez-Burks, M., Lander, A.D., Calof, A.L. (2010). Analysis of heart defects in the Nipbl+/- mouse, a model for Cornelia de Lange syndrome. Mouse Development, Genetics & Genomics 23rd Annual Meeting, Cold Spring Harbor laboratory, New York. 112. Muto A., Schilling T.F., Calof A.L., Lander, A.D. (2010) Regulation of zebrafish embryonic development by nipbl. Proceedings of the 4th Biennial Symposium of the CdLS Foundation; Am J Med Genet Part A 152A: 2687. 113. Kawauuchi S., Santos R., Lopez-Burks M., Lander A.D., Calof A.L. (2010) Analysis of Heart Defects in the Nipbl+/- Mouse, a Model for Cornelia de Lange Syndrome. Proceedings of the Mouse Molecular Genetics Meeting, CSH Laboratory, New York.

114. Muto A., Calof A., Schilling T., Lander A. (2011) Nipbl regulates organ laterality and Kupffer’s vesicle development in zebrafish. Proceedings of the 70th Society for Developmental Biology Annual Meeting, doi:10.1016/j.ydbio.2011.05.311 115. Muto A., Schilling T.F., Calof A.L., Lander A.D. (2011) Multifactorial origins of heart and gut defects in nipbl-deficient zebrafish, a model of Cornelia de Lange Syndrome. Abstracts of the Third Informal Meeting on Cohesin Biology and the Cohesinopathies, Il Ciocco, Barga, Italy. 116. Kawauchi S., Santos R., Lopez-Burks M., Jamniczky H., Qurashi S., Schostak T., Luu R., Yaramanoglu C., Yazdi M., Hallgrimsson B., Lander A.D., Calof A.L. (2011) Analysis of 40

structural birth defects in Nipbl-deficient mice. Abstracts of the Third Informal Meeting on Cohesin Biology and the Cohesinopathies, Il Ciocco, Barga, Italy. 117. Calof A.L., Gokoffski K.K., Wu H.-H., Beites C.L., Kim J., Kim, E.J., Matzuk M.M., Johnson J.E., Lander A.D. (2011) Diffferential action of activin and GDF11 on proliferation and fate choice by neural stem cells. Abstracts of the FASEB Summer Research Conference, The TGF- superfamily: Signaling in Development and Disease, Il Ciocco, Barga, Italy

118. Calof A.L., Santos R., Kawauchi S., Muto A., Lopez-Burks M., Schilling T., Lander, A.D. (2011) Vertebrate animals models of Cornelia de Lange Syndrome. Abstracts of the 8th Structural Birth Defects Meeting, NICHD, Bethesday, MD. 119. Calof A.L. (2011) Feedback, proliferation, and fate in sensory epithelium development. Abstracts of 2011 Queenstown Molecular Biology Week Development Programme, Queenstown, New Zealand 120. Calof A.L., Beites C., Gokoffski K.K., Hamilton J., Hollenbeck P., Kawauchi S., Kim J., Lopez-Burks M., Lander A.D., Santos R. (2011) Feedback, proliferation, and fate in sensory epithelium development. Abstracts of the 2011 West Coast Regional Meeting of the Society for Developmental Biology, Honolulu, Hawaii

121. Hollenbeck-Nicolosi P.L.W., Lu H.-M., Ovadia J., Greene N., Nie., Lander A., Calof A. (2011) Integration of feedback from Wnt and BMP signaling pathways modulates patterning of sensory stem cell domains in developing mouse tongue. Proceedings of the 70th Society for Developmental Biology Annual Meeting, Poster L50.

122. Hollenbeck-Nicolosi P.L.W., Ovadia J., Nie Q., Lander, A.D., Calof A.L. (2011) Feedback between Wnt and BMP signaling modulates early patterning of sensory stem cell domains in developing mouse tongue. Abstracts of the 1st Annual Southern California Systems Biology Conference, Irvine 123. Deniz, S., Santos, R., Kawauchi, S. and Calof A.L. (2011). Heart defects in a mouse model of Cornelia de Lange Syndrome. Society for the Advancement of Chicanos and Native Americans in Science (SACNAS) National Conference and Mexican American Engineers and Scientists (MEAS) Symposium 2011. San Jose, CA. 124. Kawauchi S, Santos R, Kim J, Gokoffski K, Wu H-H, Lander AD, Calof AL (2012) Genetic Interactions of Cell Intrinsic Factors and Secreted Signals Regulate Neurogenesis in Olfactory Epithelium, 45th the Japanese Association for the Study of Taste and Smell, Kanazawa, Japan. Chemical Senses. (2012) Vol. 37(4)

125. Calof, A.L., Muto, A., Santos, R., Kawauchi, S., Lopez-Burks, M., Shilling, T., and Lander, A.D. (2012). Understanding the origins of structural birth defects in CdLS using zebrafish and mouse models of Nipbl deficiency. Fifth National CdLS Scientific & Educational Symposium. Lincolnshire, IL 126. Santos, R., Kawauchi, S., Lopez-Burks, M., Muto, A., Yazdi, M., Deniz, S., Qurashi, S., Schilling, T., Lander, A.D and Calof, A.L. (2012). Conditional-invertible genetic strategy to understand the role of NIPBL deficiency in the etiology of cardiac defects in CdLS. Fifth National CdLS Scientific & Educational Symposium. Lincolnshire, IL

127. Yokomori K, Chien R, Newkirk DA, Chen Y-Y, Zeng W, Biesinger J, Infante N, Kawauchi S, Santos R, Calof AL, Lander AD, and Xie X (2012) The Effect Of Nipbl Haploinsufficiency On Genome-Wide Cohesin Binding And Gene Expression. Fifth National CdLS Scientific & Educational Symposium. Lincolnshire, IL 128. Muto A, Lopez-Burks M, Calof AL, Schilling TF, and Lander AD (2012) Potential causal factors for limb abnormalities in Cornelia de Lange Syndrome revealed by analysis of fin development in nipbl-deficient zebrafish. Proceedings of the 10th International conference on Zebrafish Development and Genetics, Madiscon, WI

129. Muto A, Lopez-Burks M, Calof AL, Schilling TF, and Lander AD (2012) Analysis of the potential origins of developmental defects in Cornelia de Lange Syndrome using zebrafish as an animal model Abstracts of the18th Japanese Medaka and Zebrafish Meeting, Kyoto, Japan 130. Calof A, Gokoffski KK, Hollenbeck-Nicolosi P, Kawauchi S, Kim J, Kunche S, Ovadia J, Santos R, Wang X, Nie Q, Lowengrub J, and Lander AD (2012) Developmental regulation and cell lineage control in sensory epithelia. Proceedings of the Sixth q-bio Conference, Santa Fe, NM 131. Calof, A.L. (2012) Developmental regulation and cell lineage control in neural development and human genetic disease. Proceedings of the First Biannual Summer School and International Conference on Neural Stem Cells in Development and Disease, Levico Terme, Trento,Italy. 132. Santos R, Wang X, Kawauchi S, Jacobs R, Lopez-Burks M, Muto A, Yazdi M, Deniz S, Qurashi S, Fraser S, Schilling T, Lander AD, and Calof AL (2012) Conditional-invertible genetic strategy to understand the role of NIPBL deficiency in the etiology of developmental defects in Cornelia de Lange Syndrome. Proceedings of the 25th Mouse Molecular Genetics Meeting, Asilomar, CA

133. Kunche S, Yan H, Lander AD, Lowengrub JS, and Calof AL (2013) Feedback Regulation of Cell Lineage Progression and the Initiation of Morphogenesis. 3rd Annual Southern California Systems Biology Conference, Irvine, CA. 134. Muto A, Lopez-Burks M, Kikuchi Y, Calof AL, Lander AD, and Schilling TF (2013) Nipbl and Med12 regulate limb-specific expression of genes cooperatively during vertebrate limb development. Abstracts of the 46th Annual Meeting of the Japanese Society for Developmental Biology, Shimane, Japan http://www.jsdb.jp/kaisai/jsdb2013/index-e.php 135. Calof AL, Muto A, Santos R, Kawauchi S, Lopez-Burks ME, Wang X, Schilling T, and Lander AD (2013) Understanding the etiology of structural birth defects using vertebrate animal models of Cornelia de Lange Syndrome. Abstracts of the NICHD 9th Structural Birth Defects Meeting , Rockville, Maryland.

136. Kunche S, Yan H, Lander AD, Lowengrub JS, and Calof AL (2013) Feedback Regualtion of Cell Lineage Progression and the Initiation of Morphogenesis. 2rd International Symposium on Systems Biology of Stem Cells, Irvine, CA. 137. Calof, AL, Santos R, Muto A, Kawauchi S, Lopez-Burks ME, Qurashi A, Yazdi M, Wang X, Yokomori K, Schilling T, Lander AD (2013) Understanding the etiology of structural birth defects using vertebrate animal models of Cornelia de Lange Syndrome. Proceedings of the 4th International Conference on Cohesin and Cohesinopathies, Certosa di Pontingano, Siena, Italy

138. Ivory Paulk, Rosaysela Santos, Shimako Kawauchi, Laura Ochikubo, Arthur Lander, and Anne Calof (2014) GABA-ergic Interneuron Deficits in a mouse Model of Cornelia de Lange Syndrome. 2014 SACNAS National Conference, Los Angeles, CA http://sacnas14abstracts.conferencespot.org/56803-sacnas-1.1394232/ivory-paulk-1.1394366 139. Anne L. Calof, Rosaysela Santos, Akihiko Muto, Shimako Kawauchi, Martha E. Lopez-Burks , Thomas Schilling2, and Arthur D. Lander (2014). Molecular and Cellular Origins of Heart and Limb Defects in Cornelia de Lange Syndrome. Abstracts of the NICHD 10th Structural Birth Defects Meeting, Rockville, Maryland.

140. Kline AD, Calof AL, Schaaf CA, Krantz ID, Jyonouchi S, Yokomori K, Gauze M,Carrico CS, Woodman J, Gerton JL, Vega H, Levin AV, Shirahige K, Champion M,Goodban MT, O'Connor JT, Pipan M, Horsfield J, Deardorff MA, Ishman SL, Dorsett D. Cornelia de Lange syndrome: further delineation of phenotype, cohesin biology and educational focus, 5th Biennial Scientific and Educational Symposium abstracts. American journal of medical genetics. Part A. 2014; 164A(6):1384-93. PubMed [journal] PMID: 24504889 141. Kline AD, Calof AL, Lander AD, Gerton JL, Krantz ID, Dorsett D, Deardorff MA, Blagowidow N, Yokomori K, Shirahige K, Santos R, Woodman J, Megee PC, O'Connor JT, Egense A, Noon S, Belote M, Goodban MT, Hansen BD, Timmons JG, Musio A, Ishman SL, Bryan Y, Wu Y, Bettini LR, Mehta D, Zakari M, Mills JA, Srivastava S, Haaland RE. Clinical, developmental and molecular update on Cornelia de Lange syndrome and the cohesin complex: abstracts from the 2014 Scientific and Educational Symposium. American journal of medical genetics. Part A. 2015; 167(6):1179-92. PubMed [journal] PMID: 25899772 142. Kline AD, Krantz ID, Deardorff MA, Shirahige K, Dorsett D, Gerton JL, Wu M, Mehta D, Mills JA, Carrico CS, Noon S, Herrera PS, Horsfield JA, Bettale C, Morgan J, Huisman SA, Moss J, McCleery J, Grados M, Hansen BD, Srivastava S, Taylor-Snell E, Kerr LM, Katz O, Calof AL, Musio A, Egense A, Haaland RE. Cornelia de Lange syndrome and molecular implications of the cohesin complex: Abstracts from the 7th biennial scientific and educational symposium 2016. American journal of medical genetics. Part A. 2017; 173(5):1172-1185. PubMed [journal] PMID: 28190301 143. Ian Krantz, Dale Dorsett , Arthur Lander, and Anne L. Calof (2017) Disorders of Transcriptional Regulation and Structural Birth Defects: Nips, Rings, CHOPS and Beyond (2- PO1HD052860) (2017)Abstracts of the NICHD 11th Structural Birth Defects Meeting, Rockville, Maryland

144. Anne Calof, Rosaysela Santos, Shimako Kawauchi, Martha E. Lopez-Burks, Phoebe Valdes, Felix Grun and Arthur Lander (2017) Using gene expression signatures to assess potential treatment strategies for Cornelia de Lange Syndrome (CdLS) using a conditional/invertible mouse model of Nipbl deficiency. Abstracts of the NICHD 11th Structural Birth Defects Meeting, Rockville, Maryland. 145. Kline AD, Krantz ID, Bando M, Shirahige K, Chea S,Sakata T, Rao S, Dorsett D, Singh V, Gerton JL, Horsfield J, Calof A, et al. (2019) Cornelia de Lange syndrome and molecular implications of the cohesin complex: Abstracts from the 8th biennial scientific and educational symposium 2018. American journal of medical genetics. Part A. 2019. 146. Santos R, Lopez-Burks M, Villagomez L, Bui B, Lander AD, and Calof AL (2019) 43

Investigating the Origins of Congenital Heart Defects in a Mouse Model of Cornelia deCornelia de Lange Syndrome. Abstracts of the Society for Experimental Biology Meeting (Abstract ID 3364), April 2019, Orlando, FL.

INVITED SEMINARS, SYMPOSIA, WORKSHOPS (since appointment at UCI only) 1995 "Factors Affecting Neuronal Birth and Death in Mammalian Olfactory Epithelium" Ciba Foundation Symposium No. 196 [Growth Factors as Drugs for Neurological and Sensory Disorders], London, United Kingdom. "Neuronal Birth and Death in the Mammalian Olfactory Epithelium" Dept. of Cell Biology and Neuroanatomy, University of Minnesota, Minneapolis, Minnesota. "Neuronal Birth and Death in the Mammalian Olfactory Epithelium" National Institute for Medical Research, London, England. "Factors Affecting Neurogenesis and Cell Death in the Mammalian Olfactory Epithelium" Gordon Conference on Neurotrophins, Plymouth, New Hampshire. "Factors Affecting Neurogenesis and Cell Death in the Mammalian Olfactory Epithelium" Second International Corsica Workshop on Molecular and Genetic Determinants of Glial and Neuronal Fate, Saint Florent, Corsica. “Neuronal Birth and Death in the Mammalian Olfactory Epithelium” Dept. of Cell and Developmental Biology, Harvard University, Cambridge, Massachusetts. “Neuronal Birth and Death in the Mammalian Olfactory Epithelium” Dept. of Biology, Brandeis University, Waltham, Massachusetts. “Neuronal Birth and Death in the Mammalian Olfactory Epithelium” Dept. of Biology, Columbia University, New York, New York. “Neuronal Birth and Death in the Mammalian Olfactory Epithelium” Dept. of Anatomy and Neurobiology, Medical College of Pennsylvania, Philadelphia, Pennsylvania. “Neuronal Birth and Death in the Mammalian Olfactory Epithelium” Dept. of Genetics and Development, Columbia University College of Physicians and Surgeons, New York, New York. 1996 "Regulating the Production of Neurons" Dept. of Biological Chemistry, University of California, Los Angeles “Regulating the Production of Olfactory Receptor Neurons” London Developmental Neurobiology Club, Guy’s Medical School, London, U.K. "Regulating the Production of Neurons in a Mammalian Sensory Epithelium" Dept. of Anatomy, University of New Mexico College of Medicine, Albuquerque New Mexico “Regulating the Production of Neurons” UCI Campuswide Symposium on Basic Cancer Research, University of California, Irvine “Regulating the Production of Neurons” Dept. of Microbiology and Molecular Genetics, University of California, Irvine

“Regulating the Production of Neurons” Dept. of Developmental and Cell Biology, University of California, Irvine 1997 "Regulating the Production of Neurons" Division of Cell and Developmental Biology, University of Texas Southwestern Medical Center, Dallas, Texas. "Regulating the Production of Neurons" Department of Cell and Neurobiology, University of Southern California School of Medicine, Los Angeles, California. “Factors Regulating Neurogenesis and Programmed Cell Death in Mouse Olfactory Epithelium” International Symposium on Olfaction and Taste XII/AChemS XIX, San Diego, California. “Intrinsic and Extrinsic Factors Regulating Vertebrate Neurogenesis” Department of Biology, University of Southern California, Los Angeles, California.

1998 "Regulating the Production of Neurons in the Olfactory Epithelium" IGBMC, CNRS/INSERM/Universite Louis Pasteur, Strasbourg, France. “Regulating the Production of Neurons: Role of BMPs in Olfactory Neurogenesis” Genetics Institute, Cambridge, Massachusetts. “Regulating the Production of Neurons: Role of BMPs in Olfactory Neurogenesis” Louisiana State University School of Medicine, New Orleans, Louisiana. 1999 "Regulation of Neuron Number in Mammalian Olfactory Epithelium” Association for Research in Otolaryngology (Symposium Presentation), St. Petersburg, Florida “Regulating the Production of Neurons: Role of BMPs in Olfactory Neurogenesis” Department of Cell and Neurobiology, University of Southern California School of Medicine, Los Angeles, California. “Regulating the Production of Neurons: Role of BMPs in Olfactory Neurogenesis” Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois. “BMPs Inhibit Neurogenesis By a Mechanism Involving Degradation of a Transcription Factor” Gordon Conference on Developmental Biology (Invited Presentation), Proctor Academy, Andover, New Hampshire. 2000 “Intrinsic and Extrinsic Factors Regulating Neurogenesis” Basic Research Seminar Series, Children’s Hospital of Los Angeles Research Institute, Los Angeles, California. “Intrinsic and Extrinsic Factors Regulating Neurogenesis in Olfactory Epithelium” Seminar Series, House Ear Institute, Los Angeles, California 2001 "Bone Morphogenetic Proteins: Multifunctional Regulators of Olfactory Neurogenesis" Gordon Conference on Chemical Senses (Symposium Presentation), Salve Regina University, Newport, Rhode Island.

"Bone Morphogenetic Proteins: Multifunctional Regulators of Neurogenesis" Department of Biology, Basic Research Seminar Series, California Institute of Technology, Pasadena, California. "Bone Morphogenetic Proteins: Multifunctional Regulators of Neurogenesis" Symposium Presentation, Ninth International Symposium on Neural Regeneration, Asilomar Conference Center, Pacific Grove, California. 2002 "Bone Morphogenetic Proteins: Multifunctional Regulators of Neurogenesis" Wyeth Pharmaceuticals, Princeton, New Jersey. "Negative Autoregulation of Neurogenesis by BMP11" Genetics Institute, Cambridge, Massachusetts "Negative Autoregulation of Neurogenesis by GDF11" Invited platform presentation, Santa Cruz Conference on Developmental Biology, Santa Cruz, California. "Negative Autoregulation of Neurogenesis by GDF11" Invited platform presentation, Gordon Conference on Neural Development, Newport, Rhode Island.

2003 "Regulation of Neurogenesis in Mammals", Dept. of Physiology and Biophysics Seminar Series, University of Washington, Seattle, Washington. "Studies of the Neuronal Stem Cell of the Olfactory Receptor Neuron Lineage" Invited Symposium Presentation, 26th Midwinter Research Meeting, Assoc. for Research in Otolaryngology, Daytona Beach, FL "Feedback Regulation of Neurogenesis", Invited platform presentation, International Symposium on Developmental Biology, Juan March Foundation, Madrid, Spain, June 2003 "Feedback Regulation of Neuron Number", Platform Presentation, Children's Hospital of Los Angeles Research Institute Symposium on Organogenesis (Inaugural Symposium of the Sabin Research Institute), Los Angeles, California. "Feedback Regulation of Neuron Number" Department of Biology Seminar Series, University of Southern California, Los Angeles, California.

2004 "Molecular Regulation of Neurogenesis in the Olfactory Receptor Neuron Lineage" Department of Physiology Seminar Series, University of Kentucky, Lexington, KY "Common Molecular Signals Regulating Progression through the Neuronal Lineage in Olfactory and Other Sensory Epithelia" , Symposium Presentation, Annual Meeting, Association for Chemoreception Sciences, Sarasota, FL "GDF11 and Follistatin Interact to Govern Cell Number and Cell-Type Determination in Developing Mouse Nervous System" Platform presentation. Stem Cells Session, Santa Cruz Conference on Developmental Biology, Santa Cruz, CA "Molecular Signals Regulating Development of Mammalian Neural Stem Cells". Platform Presentation, Howard Schneiderman Memorial Bioethics Lecture Series, "Developing Stem 46

Cell Therapies", University of California, Irvine

2005 "Common Molecular Signals Regulating Neurogenesis in the Olfactory Epithelium and other Sensory Epithelia" Center for Molecular Neuroscience, Vanderbilt University, Nashville, TN "Common Molecular Signals Relating Progression through the Neuronal Lineage in Olfactory Epithelium and other Sensory Epithelia" Symposium Presentation, Gordon Conference on Epithelial Differentiation and Keratinization, Il Ciocco, Barga, Italy "How Studying the Mouse Can Provide Us with Clues for Understanding Neural Stem Cells and Human Genetic Disease" Keynote Speaker, University of California, Davis, Annual Neuroscience Retreat, Fallen Leaf Lake, CA " GDF11 Regulates Cell Number and Cell Fate Determination in Developing Mouse Retina" Symposium Presentation, ARVO Western Eye Research Conference on Regenerative Ocular Biology: Restoring Vision in the Aged and Diseased Eye, Laguna Beach, CA "How Studying the Mouse Can Provide Us with Clues for Understanding Neural Stem Cells and Human Genetic Disease" Rutgers University Distinguished Women In Neuroscience Seminar Series, Rutgers University, Piscataway, New Jersey "How Studying the Mouse Can Provide Us with Clues for Understanding Neural Stem Cells and Human Genetic Disease", Departmental Seminar Series, Dept. of Cell Biology, Neurobiology and Anatomy. Medical College of Wisconsin, Milwaukee, WI "Neuronal and Glial Lineages in the Primary Olfactory System -- How Many Stem Cells?" Invited Lecture, Advanced Cell Biology Course, Interdisciplinary Graduate Program, Medical College of Wisconsin, Milwaukee, WI "Common Molecular Signals Regulating Neurogenesis in Sensory Epithelia" Invited Lecture, Merck Pharmaceuticals, West Point, PA "Common Molecular Signals Regulating Neurogenesis in Sensory Epithelia" Grand Rounds, Dept. of Ophthalmology, University of California School of Medicine, Irvine, CA

2006 "Common Molecular Signals in Olfactory Neurogenesis and Craniofacial Morphogenesis", Symposium Presentation, Gordon Conference on Craniofacial Morphogenesis and Tissue Regeneration, Ventura, CA "Requirement for Fgf8 in Primary Olfactory Neurogenesis and Nasal Cavity Morphogenesis", Symposium Presentation, Gordon Conference on Fibroblast Growth Factors in Development and Disease, Ventura, CA "Role of TGF-beta Superfamily Signaling in Retinal Cell-Type Specification" Symposium 47

Presentation, Visual System Development Gordon Research Conference, Il Ciocco, Barga, Italy "Regulation of Neurogenesis in Mammals" Molecular Biology Formal Seminar Series, University of California, Los Angeles, School of Medicine, Los Angeles, CA "Regulation of Neurogenesis in Mammals" Program in Biological Sciences, Formal Neuroscience Seminar Series, University of California, San Francisco, School of Medicine, San Francisco, CA "Regulation of Neurogenesis in Mammals" Departmental Seminar Series, Dept of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara

2007 "Regulation of Neurogenesis in Mammals" Stem Cell Seminar Series, Duke University School of Medicine, Raleigh-Durham, North Carolina. "Regulation of Neurogenesis in Mammals" Blaffer Endowed Lectureship, M.D. Anderson Cancer Center of the University of Texas, Houston, Texas "Regulation of Neurogenesis in Mammals" Neuroscience Seminar Series, University of Pittsburgh, Pittshburgh, Pennsylvania. “Common Molecular Signals Regulating Neurogenesis in Olfactory Epithelium and Neural Retina”, Invited Presentation, London Developmental Neurobiology Club, Guy’s Hospital, London, England. “Developmental and Neurological Phenotypes of Cornelia de Lange Syndrome: Insights from the Nipbl Mutant Mouse”, Platform Presentation, First Meeting on Cohesin Biology and the Cohesinopathies, Varenna, Italy. “Can Developmental Signaling Molecules Be Harnessed to Promote Retinal Regneration from Endogenous Stem Cells?” Platform Presentation, University of California, Irvine, Eye Institute 2nd Annual Collaboration Colloquium, University of California, Irvine. “Developmental and Neurological Phenotypes of Cornelia de Lange Syndrome: Insights from the Nipbl Mutant Mouse” Platform Presentation, 4th CdLS World Conference, Niagara Falls, Canada “Common Molecular Signals Regulating Neurogenesis in Sensory Epithelia”, Platform Presentation, 25th Reunion Symposium, CSH Molecular Embryology of the Mouse Course, Cold Spring Harbor Laboratory, New York. “Can Developmental Signaling Molecules Be Harnessed to Promote Retinal Regeneration from Endogenous Stem Cells?” Platform Presentation, Pacific Ocular Regenerative Biology Conference X11, Laguna Beach, CA. “Common Molecular Signals Regulating Neurogenesis in Sensory Epithelia”, Regular Seminar Series, School of Biological Sciences, University of Liverpool, Liverpool, England. 48

“Mouse Models for Studying Neural Stem Cell Development and Human Genetic Disease”. Departmental Seminar, MRC Centre for Developmental Neurobiology, King's College London, London, UK.

2008 “Using a Model Genetic Organism to Investigate the Molecular Basis of Hearing Defects in Inherited Disease”, Platform presentation, UCI Center for Hearing Research 2008 Fall Community Outreach Symposium on Hearing Research, Beckman National Academy of Sciences, University of California, Irvine “Modeling early developmental defects in Cornelia de Lange Syndrome using the zebrafish” Platform Presentation, Third National CdLS Foundation Scientific Symposium, Chicago, Illinois “Insights into Cornelia de Lange Syndrome from the Nipbl-mutant mouse” Platform Presentation, Third National CdLS Foundation Scientific Symposium, Chicago, Illinois “Stem cells in sensory epithelium development and regeneration”, Symposium, 15th International Symposium on Olfaction and Taste. San Francisco, California

2009 “Insights into Cornelia de Lange Syndrome from the Nipbl-mutant mouse, Platform Presentation, Second International Meeting on Cohesin Biology and the Cohesinopathies, Certosa di Pontignano, Siena, Italy “Mouse Models for Studying Neural Stem Cell Development and Human Genetic Disease”, Centre for Integrative Biology, CIBIO, University of Trento, Trento, Italy “Insights into Cornelia de Lange Syndrome from the Nipbl-mutant mouse”, Platform Presentation, Sanger Institute/CSHL Joint meeting on Mouse Genetics and genomics; Development & Disease, Hinxton, Cambridge, United Kingdom “Mouse Models for Studying Neural Stem Cell Development and Human Genetic Disease”. Departmental Seminar Series, Department of Biology, University of Iowa, Iowa City, Iowa

2010 "Using the Mouse as a Model Organism for Studying Neural Stem Cell Development and Human Genetic Disease", UCI Stem Cell Symposium (International Stem Cell Awareness Day) -- Platform Presentation/ "Meet the Scientists", Irvine, California “Mouse Models for Studying Neural Stem Cell Development and Human Genetic Disease”. Program in Neuroscience, Invited Seminar, University of Washington, Seattle, Washington

“Etiology of Birth Defects in Cornelia de Lange Syndrome: Insights from studies of mouse and Zebrafish Model Systems”, Invited Platform Presentation, Fourth National Cornelia de Lange Syndrome Scientific Symposium, Dallas, Texas

2011 "Feedback, Proliferation, and Fate in the Retina", Vision Discovery Institute, Distinguished Speaker Seminar Series, Georgia Health Sciences University, Augusta, Georgia "Feedback, Proliferation, and Fate in Sensory Epithelium Development", Plenary Speaker, Society for Developmental Biology West Coast Regional Meeting, University of Hawaii, Honolulu, Hawaii "Using the Mouse as a Model Organism for Studying Neural Stem Cell Development and Human Genetic Disease", Killam Distinguished Speaker Seminar Series, Montreal Neurological Sciences Institute, Montreal, Canada "Using the Mouse as a Model Organism for Studying Neural Stem Cell Development and Human Genetic Disease", Public Lecture Series, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, California "Vertebrate Animal Models of Cornelia de Lange Syndrome", National Institute of Child Health and Human Disease, Eight Structural Birth Defects Meeting, Rockville, Maryland "Differential Actions of Activin and GDF11 on Proliferation and Fate Choice by Neural Stem Cells", Platform Presentation, FASEB Summer Research Conference 'The TGF-beta Superfamily" Signaling in Development and Disease', Il Ciocco, Barga, Italy "Feedback, Proliferation, and Fate in Sensory Epithelium Development", Platform Presentation, Queenstown Molecular Biology Week, Queenstown, New Zealand "Isabel's Web: What We Can Learn from Studying Mouse and Animal Models of Cornelia de Lange Syndrome", Cornelia de Lange Syndrome Foundation, Orange County Reception for CdLS Foundation USA, Costa Mesa, California

2012 "Insights into Developmental Regulation: From Cell Lineage Control to Birth Defects”, Department of Physiology, Yale University School of Medicine, New Haven, Connecticut. “Understanding the Origins of Structural Birth Defects in Cornelia de Lange Syndrome Using Zebrafish and Mouse Models of NIPBL Deficiency”, Platform Presentation, Fifth National CdLS Scientific and Educational Symposium, Cornelia de Lange System Foundation National Conference, Chicago, Illinois “Developmental Regulation and Cell Lineage Control in Sensory Epithelia”, Platform Presentation, The Sixth Annual q-bio Conference, Santa Fe, New Mexico 50

“Developmental Regulation and Cell Lineage Control in Neural Development and Human Genetic Disease”, Platform Presentation, Neural Stem Cells in Development and Disease (First International Conference and Summer School), Levico Terme, Trento, Italy "Insights into Developmental Regulation: From Cell Lineage Control to Birth Defects”, Department of Genetics, Yale University School of Medicine, New Haven, Connecticut (October 18, 2012) “Insights into Regulation of Neural Development: From Cell Lineage Control to Birth Defects”, Platform Presentation, Symposium on Stem Cell Technologies in Functional Neurogenomics, Lieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, Maryland (November 27, 2012)

2013 “Understanding the etiology of structural birth defects using vertebrate animal models of Cornelia de Lange Syndrome”, NICHD 9th Structural Birth Defects Meeting, Rockville, MD (April, 2013) (Invited platform presentation) “Multimodal feedback in lineage control and morphogenesis”, Invited platform presentation, Second International Symposium on Systems Biology of Stem Cells, Beckman Center of the National Academies of Science, Irvine, CA (June, 2013) “Feedback control of lineage progression in morphogenesis and cell fate choice” Invited platform presentation, Gordon Research Conference on Neural Crest and Placodes: Concepts, Mechanisms, and Models, Stonehill College, Easton MA (July 2013) 2014 “Using the Mouse as a Model Organism for Studying Retinal Stem Cell Development and and Human Genetic Disease”, The Gavin Herbert Eye Institute Discovery Center for Eye Research “Bench to Bedside Symposium”, Irvine, California, May 2014 “Origins of Congenital Heart Defects in Mouse Models of CdLS”, Invited Platform presentation, Sixth National Cornelia de Lange Syndrome Scientific and Educational Symposium, Costa Mesa, California, June 2014 "Isabel's Web: What We Can Learn from Studying Mouse and Animal Models of Cornelia de Lange Syndrome", Cornelia de Lange Syndrome Foundation, Orange County Reception, Costa Mesa, California. Opening presentation, CdLS Foundation Family Conference, Costa Mesa, California, June 2014 “Molecular and Cellular Origins of Heart and Limb Defects in Cornelia de Lange Syndrome”, National Institute for Child Heath and Developmental Disorders, 10th Structural Birth Defects Meeting, Bethesda, Maryland, December 2014 2015 “Insight into the Regulation of Neural Development: From Cell Lineage Control to Birth Defects”, Invited presentation, Intellectual and Developmental Disorders Research Center 51

(IDDRC), The Childrens Research Institute, Children’s National Medical Center and George Washington University, Washington, DC (January 2015) “On Being a Scientist and Professor of Biology at UC Irvine”, Career Day, Thurston Middle School, Laguna Beach, California (October 14, 2015) “Insight into Developmental Regulation: From Cell Lineage Control to Birth Defects”, Invited seminar, Fuzhou University Institute of Life Sciences, Fuzhou, China (December, 2015) 2016 “Interactions Within and Between Stem Cell Linages: From Morphogenesis to Birth Defects”, Invited Platform Presentation, 12th Annual Stem Cell Symposium, “Stem Cells and the Niche”, Eli and Edythe Broad Center Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA (February 2016) “Utilizing Gene Expression Signatures to Assess Treatment Strategies for Cornlia de Lange Syndrome Using a Conditional/Invertible Mouse Model of Nipbl Deficiency”, Invited Platform presentation, Seventh National Cornelia de Lange Syndrome Scientific and Educational Symposium, Orlando, Florida, (June 2016) “Using the Mouse to Model the Origins of Birth Defects in Transcriptomopathies”, Invited Seminar (Regular Seminar Series), Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan (July, 2016) 2017 “Transcriptional Regulation and Structural Birth Defects: Nips, Rings, CHOPS and Beyond - Using conditional creation and rescue of Nipbl deficiency in mice to understand the origins of congenital heart defects in CdLS”, Invited Platformresentation, National Institute for Child Heath and Developmental Disorders and Society for Developmental Biology, 11th Structural Birth Defects Meeting, FASEB Conference Center, Bethesda, Maryland, April 2017 “Molecular approaches for assessing treatment strategies for Cornelia de Lange Syndrome (CdLS) using a conditional/invertible mouse model of Nipbl deficiency”, Platform presentation, National Institute for Child Heath and Developmental Disorders and Society for Developmental Biology, 11th Structural Birth Defects Meeting, FASEB Conference Center, Bethesda, Maryland, April 2017 “Transcriptomopathies: A Window on the Origins of Birth Defects”, Invited Seminar (Regular Seminar Series), Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan (July, 2017) “Academic Training and Career Opportunities in Biomedical Sciences in the United States”, Invited presentation to GIP-TRIAD (Global Innovation Joint Degree Program) Master’s students, National Taiwan University School of Medicine, Taipei, Taiwan (July 2017) “Developmental Regulation and Cell Lineage Control in Sensory Epithelia and Human Genetic Disease, Graduate Institute of Brain and Mind Sciences, National Taiwan University, Taipei, Taiwan (August 2017)

2018 “Towards a Rational Approach to Identify Potential Targets for Pharmacological Therapies for Cornelia de Lange Syndrome”, Eight Biennial Cornelia de Lange Syndrome Scientific and Educational Symposium, Minneapolis, Minnesota (June 2018) “Genetic Routes to Discovery in Developmental Neuroscience”, National Taiwan University, Graduate Institute of Brain and Mind Sciences and Center for Biotechnology, Taipei, Taiwan (August 2018)