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Datasheet A07954-1 Anti-DNAJC10 Antibody

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Datasheet A07954-1 Anti-DNAJC10 Antibody Product datasheet Anti-DNAJC10 Antibody Catalog Number: A07954-1 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] Basic Information Product Name Anti-DNAJC10 Antibody Gene Name DNAJC10 Source Rabbit IgG Species Reactivity human, mouse, rat Tested Application WB,IHC-P,ICC/IF,FCM,Direct ELISA Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E.coli-derived human DNAJC10 recombinant protein (Position: D33-L793). Purification Immunogen affinity purified. Observed MW 91KD Dilution Ratios Western blot: 1:500-2000 Immunohistochemistry in paraffin section IHC-(P)： 1:50-400 Immunocytochemistry/Immunofluorescence (ICC/IF): 1:50-400 Flow cytometry (FCM)： 1-3μg/1x106 cells Direct ELISA： 1:100-1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing Background Information DnaJ homolog subfamily C member 10 is a protein that in humans is encoded by the DNAJC10 gene. This gene encodes an endoplasmic reticulum co-chaperone which is part of the endoplasmic reticulum-associated degradation complex involved in recognizing and degrading misfolded proteins. The encoded protein reduces incorrect disulfide bonds in misfolded glycoproteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Reference Anti-DNAJC10 Antibody被引用在0文献中。 暂无引用 FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-DNAJC10 Antibody Catalog Number: A07954-1 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] Selected Validation Data Figure 1. Western blot Figure 2. IHC analysis analysis of anti- DNAJC10 using anti- DNAJC10 antibody (A07954-1). The antibody (A07954-1). sample well of each lane detected in paraffin- was loaded with 50ug of embedded section of sample under reducing human breast cancer conditions. Lane 1: tissue. Biotinylated goat human HELA whole cell anti-rabbit IgG was used lysates, Lane 2: human as secondary antibody. HEPG2 whole cell lysates, The tissue section was Lane 3: human U87 whole developed using Strepavi cell lysates, Lane 4: rat din-Biotin-Complex stomach tissue lysates, (SABC) (Catalog # Lane 5: rat RH35 whole SA1022) with DAB as the cell lysates, Lane 6: chromogen. mouse stomach tissue lysates, Lane 7: mouse HEPA1-6 whole cell lysates. Use rabbit anti- DNAJC10 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for DNAJC10 at approximately 91KD. The expected band size for DNAJC10 at 91KD. Figure 3. IHC analysis Figure 4. IHC analysis using anti- DNAJC10 using anti- DNAJC10 antibody (A07954-1). antibody (A07954-1). detected in paraffin- detected in paraffin- embedded section of embedded section of human placenta tissue. human prostate cancer Biotinylated goat anti- tissue. Biotinylated goat rabbit IgG was used as anti-rabbit IgG was used secondary antibody. The as secondary antibody. tissue section was The tissue section was developed using Strepavi developed using Strepavi din-Biotin-Complex din-Biotin-Complex (SABC) (Catalog # (SABC) (Catalog # SA1022) with DAB as the SA1022) with DAB as the FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2 Product datasheet Anti-DNAJC10 Antibody Catalog Number: A07954-1 BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected] chromogen. chromogen. FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 3 Powered by TCPDF (www.tcpdf.org).
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