ELMO2 Polyclonal Antibody

ELMO2 polyclonal antibody

Catalog # : PAB6106 規格 : [ 100 ug ] List All

Specification Application Image

Product Goat polyclonal antibody raised against synthetic peptide of ELMO2. Western Blot (Tissue lysate) Description:

Immunogen: A synthetic peptide corresponding to C-terminus of human ELMO2.

Sequence: C-PKEPSSYDFVYHYG enlarge Host: Goat Immunohistochemistry Theoretical MW 82.5 (Formalin/PFA-fixed paraffin- (kDa): embedded sections)

Reactivity: Human

Specificity: NP_877496.1 and NP_57340.3.1 are varients that encode the same protein. enlarge Form: Liquid ELISA Purification: Antigen affinity purification

Concentration: 0.5 mg/mL

Recommend ELISA (1:32000) Usage: Western blot (1-3 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-6 ug/mL) The optimal working dilution should be determined by the end user.

Storage Buffer: In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)

Storage Store at -20°C. Instruction: Aliquot to avoid repeated freezing and thawing.

Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Datasheet: Download

Publication Reference 1. CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration. Gumienny TL, Brugnera E, Tosello-Trampont AC, Kinchen JM, Haney LB, Nishiwaki K, Walk SF, Nemergut ME, Macara IG, Francis R, Schedl T, Qin Y, Van Aelst L, Hengartner MO, Ravichandran KS.Cell. 2001 Oct 5;107(1):27-41.

Applications Western Blot (Tissue lysate)

Page 1 of 3 2017/2/16 ELMO2 polyclonal antibody (Cat # PAB6106) staining (2 ug/mL) of human brain lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

ELMO2 polyclonal antibody (Cat # PAB6106)(3.8 ug/mL) staining of paraffin embedded human prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

ELISA

Gene Information

Entrez GeneID: 63916

Protein NP_877496.1;NP_57340.3.1 Accession#:

Gene Name: ELMO2

Gene Alias: CED-12,CED12,ELMO-2,FLJ11656,KIAA1834

Gene engulfment and cell motility 2 Description:

Omim ID: 606421

Gene Ontology: Hyperlink

Gene Summary: The protein encoded by this gene interacts with the dedicator of cyto- kinesis 1 protein. Similarity to a C. elegans protein suggests that this protein may function in phagocytosis of apoptotic cells and in cell migration. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq

Other OTTHUMP00000031762,PH domain protein CED12A,ced-12 homolog 2 Designations:

Related Disease Diabetes Mellitus, Type 2 Diabetic Nephropathies Genetic Predisposition to Disease Kidney Failure, Chronic

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