Isolation and Structure Elucidation of Antiproliferative
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Direct Analysis of Hops by Leaf Spray and Paper Spray Mass Spectrometry
Western Michigan University ScholarWorks at WMU Master's Theses Graduate College 12-2012 Direct Analysis of Hops by Leaf Spray and Paper Spray Mass Spectrometry Kari Blain Follow this and additional works at: https://scholarworks.wmich.edu/masters_theses Part of the Chemistry Commons Recommended Citation Blain, Kari, "Direct Analysis of Hops by Leaf Spray and Paper Spray Mass Spectrometry" (2012). Master's Theses. 100. https://scholarworks.wmich.edu/masters_theses/100 This Masters Thesis-Open Access is brought to you for free and open access by the Graduate College at ScholarWorks at WMU. It has been accepted for inclusion in Master's Theses by an authorized administrator of ScholarWorks at WMU. For more information, please contact [email protected]. DIRECT ANALYSIS OF HOPS BY LEAF SPRAY AND PAPER SPRAY MASS SPECTROMETRY Kari Blain, M.S. Western Michigan University, 2012 The objective of this research is to develop a new and innovative method of hops analysis, which is much faster than standard testing methods, as well as reduce the amount of consumables and solvent used. A detailed discussion on the development of an ambient ionization mass spectrometry method called paper spray (PS-MS) and leaf spray (LS-MS) mass spectrometry will be presented. This research investigates the use of PS-MS and LS-MS techniques to determine the α- and β- acids present in hops. PS-MS and LS-MS provide a fast way to analyze hops samples by delivering data as rapidly as a UV-Vis measurement while providing information similar to lengthy liquid chromatographic separations. The preliminary results shown here indicate that PS-MS could be used to determine cohumulone and α/β ratios. -
ACE Food Beverage Applications Guide
Food and Beverage Applications Guide Ultra-Inert Base Deactivated UHPLC / HPLC Columns Contents Application Index 1 Analyte Index 2 - 4 Application Notes 5 - 90 Send us your application and receive a free ACE column Send us your application on an ACE column and help extend our applications database. Your proven method will enable your chromatography colleagues to benefit and if we select your application for publication we’ll send you a FREE ACE analytical column of your choice. To submit your application e-mail us at: [email protected] ACE ® Food and Beverage Applications: Application Index Application Pages Application Pages Additives and intense sweeteners 5 Organic acids 2 47 Argicultural pesticides 6 Organic acids 3 48 Amino acids in peas 7 Organic acids 4 49 Amino acids and biogenic amines in wine and beer 8 Organophosphorus flame retardants in water by LC-MS/MS 50 Aminoglycosides in eggs 9 Organophosphorus isomer flame retardants in water 51 Annatto 10 Paraben preservatives 52 Anthocyanins from sambucus nigra (elderberry) 11 Perfluoro acids by LC-MS/MS 53 Appetite suppressants by LC-MS 12 Perfluoroalkyl substances by ion pairing LC-MS/MS 54 Arsenolipids from edible seaweed 13 Perfluorinated compounds in water by LC-MS/MS 55 Artificial colours (water soluble) 14 Pesticides (250 analytes) by LC-MS/MS 56 Artificial food colouring 15 Pesticides (47 analytes) by LC-MS/MS 60 Artificial sweeteners global method 16 Pesticides in water 61 Artificial sweeteners (stevia glycosides) 17 Phenolic compounds in ground water and landfill leachates -
Masterarbeit / Master's Thesis
MASTERARBEIT / MASTER’S THESIS Titel der Masterarbeit / Title of the Master‘s Thesis Optimization of an LC-MS/MS Method for the Determination of Xenobiotics in Biological Matrices verfasst von / submitted by Thomas Jamnik BSc angestrebter akademischer Grad / in partial fulfilment of the requirements for the degree of Master of Science (MSc) Wien, 2020 / Vienna 2020 Studienkennzahl lt. Studienblatt / UA 066 863 degree programme code as it appears on the student record sheet: Studienrichtung lt. Studienblatt / Masterstudium Biologische Chemie degree programme as it appears on the student record sheet: Betreut von / Supervisor: Assoz. Prof. Dipl.-Ing. Dr. Benedikt Warth, Bakk.techn. 1 2 Erklärung Ich erkläre, dass die vorliegende Masterarbeit von mit selbst verfasst wurde und ich keine anderen als die angeführten Behelfe verwendet bzw. mich auch sonst keiner unerlaubter Hilfe bedient habe. Ich versichere, dass diese Arbeit bisher weder im In- noch Ausland in irgendeiner Form als Prüfungsarbeit vorgelegt wurde. Ich habe mich bemüht, sämtliche Inhaber der Bildrechte ausfindig zu machen und ihre Zustimmung zur Verwendung der Bilder in dieser Arbeit eingeholt. Sollte dennoch eine Urheberrechtsverletzung bekannt werden, ersuche ich um Meldung bei mir. Danksagung Ich danke Dr. Benedikt Warth nicht nur für die Möglichkeit diese interessante Masterarbeit verfassen zu dürfen, sondern auch für die gewonnenen Erfahrungen die der Einblick in seine Arbeitsgruppe und das Institut für Lebensmittelchemie erlaubt hat. Besonderer Dank gilt meiner direkten Betreuerin Dipl.-Ing. Mira Flasch, welche stets hilfsbereite Unterweisung in die Praxis als auch Theorie der verwendeten Arbeitsmethoden gab, immer für ausgiebige Diskussionen bereit stand und sich viel Zeit für diverse Korrekturen dieser Arbeit nahm. -
Environmental Chemical Test Results Prepared For: Anjuli S
Environmental Chemical Test Results Prepared for: Anjuli S. th Sample collection date: October 5 , 2020 Pg 1 of 23 Environmental Chemical Test Results Prepared for: Sample Collection Date: th Anjuli S. October 5 , 2020 Below are the results for your exposures to everyday toxic chemicals and phytoestrogens. This includes your personalized recommendations based on your lifestyle audit and exposures. This information is for educational purposes only and is not intended to diagnose or treat any health conditions. Contact us for questions or feedback, or see the FAQs for answers to general questions about your test. Report Table of Contents BISPHENOLS 2 PARABENS 5 PHTHALATES 10 OTHER EVERYDAYSample TOXIC CHEMICALS 14 MYCOTOXINS 19 PHYTOESTROGENS 21 Environmental Chemical Test Results Prepared for: Anjuli S. th Sample collection date: October 5 , 2020 Pg 2 of 23 BISPHENOLS Bisphenol A Test Results About Click here for information about sources of exposure and health effects. Test Results Total Bisphenol A (BPA) = Not Detected* Your level was not-detected, indicating LOW levels of exposure. Report Sample Environmental Chemical Test Results Prepared for: Anjuli S. th Sample collection date: October 5 , 2020 Pg 3 of 23 Bisphenol S Test Results About Click here for information about sources of exposure and health effects. Test Results Total Bisphenol S (BPS) = Not Detected* Your level was not-detected, indicating LOW levels of exposure. Report Sample **SAMPLE RECOMMENDATIONS--Your report will be more detailed and tailored to you.** Take Action: Bisphenols Great job! You have low exposures to BPA and BPS. To keep these exposures low: 1. Avoid “Proposition 65” products. -
Determination of Isoxanthohumol, Xanthohumol, Alpha and Beta Bitter
Determination of Isoxanthohumol, Xanthohumol, Alpha and Beta Bitter Acids, and trans and cis-Iso-Alpha Acids by HPLC with UV and Electrochemical Detection: Application to Hop and Beer Analysis Paul A. Ullucci, Ian N. Acworth, and David Thomas Thermo Fisher Scientific, Chelmsford, MA, USA Data Analysis Polyphenol Method – Targeted Analysis FIGURE 2. Principal Component Plots for A) ECD and B) UV Data. TABLE 2. Hops Bitter Acids Data Presented in mg/L. Overview 1 Data were analyzed using Thermo Fisher Dionex Chromeleon Chromatography Data The analytical figures of merit for this assay were described preciously. Briefly, the Beer 1 Beer 2 Beer 3 Beer 4 Purpose: To develop gradient HPLC methods using a spectro-electro array platform for use System 6.8 (SR 9) and CoulArray™ software 3.1. EC-array data were transferred to limits of detection were typically 10−50 pg on column by ECD and 100−500 pg by UV. Beer 7 Compound Ultra IPA Ultra IPA Regular Light Beer to either measure specific analytes in beer samples or in a metabolomic approach to Pirouette® software for chemometric analysis using a CoulArray version 2.0 Software The limits of quantification were 200−1000 pg on column by ECD and 500−5000 pg by A distinguish between different beer samples, as well as study beer stability. Utility (Pattern Recognition Setup Wizard). UV data were tabularized prior to transfer to UV. Response range was over seven orders of magnitude by ECD and five by UV. Factor2 Ultra Isoxanthohumol 2.10 1.3 0.38 0.28 2 Methods: Gradient HPLC with diode-array detection and electrochemical array detection Pirouette. -
Dry Forest Trees of Madagascar
The Red List of Dry Forest Trees of Madagascar Emily Beech, Malin Rivers, Sylvie Andriambololonera, Faranirina Lantoarisoa, Helene Ralimanana, Solofo Rakotoarisoa, Aro Vonjy Ramarosandratana, Megan Barstow, Katharine Davies, Ryan Hills, Kate Marfleet & Vololoniaina Jeannoda Published by Botanic Gardens Conservation International Descanso House, 199 Kew Road, Richmond, Surrey, TW9 3BW, UK. © 2020 Botanic Gardens Conservation International ISBN-10: 978-1-905164-75-2 ISBN-13: 978-1-905164-75-2 Reproduction of any part of the publication for educational, conservation and other non-profit purposes is authorized without prior permission from the copyright holder, provided that the source is fully acknowledged. Reproduction for resale or other commercial purposes is prohibited without prior written permission from the copyright holder. Recommended citation: Beech, E., Rivers, M., Andriambololonera, S., Lantoarisoa, F., Ralimanana, H., Rakotoarisoa, S., Ramarosandratana, A.V., Barstow, M., Davies, K., Hills, BOTANIC GARDENS CONSERVATION INTERNATIONAL (BGCI) R., Marfleet, K. and Jeannoda, V. (2020). Red List of is the world’s largest plant conservation network, comprising more than Dry Forest Trees of Madagascar. BGCI. Richmond, UK. 500 botanic gardens in over 100 countries, and provides the secretariat to AUTHORS the IUCN/SSC Global Tree Specialist Group. BGCI was established in 1987 Sylvie Andriambololonera and and is a registered charity with offices in the UK, US, China and Kenya. Faranirina Lantoarisoa: Missouri Botanical Garden Madagascar Program Helene Ralimanana and Solofo Rakotoarisoa: Kew Madagascar Conservation Centre Aro Vonjy Ramarosandratana: University of Antananarivo (Plant Biology and Ecology Department) THE IUCN/SSC GLOBAL TREE SPECIALIST GROUP (GTSG) forms part of the Species Survival Commission’s network of over 7,000 Emily Beech, Megan Barstow, Katharine Davies, Ryan Hills, Kate Marfleet and Malin Rivers: BGCI volunteers working to stop the loss of plants, animals and their habitats. -
Simultánní Stanovení Prenylflavonoidů a Isoflavonoidů Ve Chmelu a Pivu
KVASNY PRUM. Simultánní stanovení prenylflavonoidů a isoflavonoidů ve chmelu a pivu ... 59 / 2013 (2) 41 Simultánní stanovení prenylflavonoidů a isoflavonoidů ve chmelu a pivu metodou HPLC-DAD: Studie aplikace homogenátu zeleného chmele v pivovarském procesu Simultaneous Determination of Prenylflavonoids and Isoflavonoids in Hops and Beer by HPLC-DAD Method: Study of Green Hops Homogenate Application in the Brewing Process MARIE JURKOVÁ 1, Pavel ČEJKA 1, MILAN HOUŠKA 2, ALExANDR MIKYŠKA 1 1 Výzkumný ústav pivovarský a sladařský, a.s., Pivovarský ústav Praha, Lípová 15,120 44 Praha 2 / Research Institute of Brewing and Malting PLC, Brewing Institute Prague, Lípová 15, CZ – 120 44 Prague 2, Czech Republic 2 Výzkumný ústav potravinářský Praha / Food Research Institute Prague – Rádiová 7, CZ – 102 31, Prague 10, Czech Republic e-mail: [email protected] Jurková, M. – Čejka, P. – Houška, M. – Mikyška, A.: Simultánní stanovení prenylflavonoidů a isoflavonoidů ve chmelu a pivu metodou HPLC-DAD: Studie aplikace homogenátu zeleného chmele v pivovarském procesu. Kvasny Prum . 59, 2013, č . 2, s . 41–49 . Polyfenolové látky náležející do skupin prenylflavonoidů a isoflavonoidů mají účinky podporující zdraví a ochranu proti řadě civilizač- ních chorob . Působí jako antioxidanty, mají protirakovinné, antimikrobiální, protizánětlivé vlastnosti, některé jsou fytoestrogeny a půso- bí proti osteoporóze . Byla vypracována nová analytická metoda HPLC-DAD pro simultánní stanovení prenylflavonoidů (xanthohumol, isoxanthohumol, 8-prenylnaringenin, 6-prenylnaringenin) a isoflavonoidů (daidzein, genistein, formononetin a Biochanin A) ve chmelu a pivu a byla provedena studie dopadu chmelení homogenátem zeleného chmele stabilizovaného vysokým tlakem na obsah těchto látek v pivu . Vypracovaná metoda může najít uplatnění při monitorování obsahu bioaktivních flavonoidů ve chmelových surovinách a pivu . -
Highly Isoxanthohumol Enriched Hop Extract Obtained by Pressurized Hot
ACCEPTED MANUSCRIPT HIGHLY ISOXANTHOHUMOL ENRICHED HOP EXTRACT OBTAINED BY PRESSURIZED HOT WATER EXTRACTION (PHWE). CHEMICAL AND FUNCTIONAL CHARACTERIZATION. Alicia Gil-Ramíreza; José Antonio Mendiolab; Elena Arranza; Alejandro Ruíz- Rodrígueza; Guillermo Regleroa; Elena Ibáñez; Francisco R Marína* a Department of Characterization and production of New Foods. b Department of Bioactivity and Food Analysis. Institute of Food Science Research (CIAL), (Spanish National Research Council–Universidad Autónoma de Madrid), C/Nicolás Cabrera, 9. Campus de la Universidad Autónoma de Madrid, 28049 Madrid, Spain. * Corresponding author: [email protected] +34910017921 ACCEPTED MANUSCRIPT 1 ACCEPTED MANUSCRIPT ABSTRACT Hop (Humulus lupulus) is one of the richest natural sources of a prenylfalvonoids such as xanthohumol (XN), desmetylxanthohumol (DMX), isoxanthohumol (IX) or 8- prenylnaringenin (8-PN), being XN the most abundant of them in the raw material. So far, obtention of prenylflavonoids have been done by chemical synthesis or extraction with organic solvents, with no described methods for the isolation of IX, which has been reported to have anti-inflammatory properties. In this study, pressurized hot water extraction (PHWE) it is shown not only as effective method to extract some prenylflavonoids but to selectively change the relative amount of them, favoring the extraction of IX against XN. Thus, pressurized water extraction at 150ºC showed a high selectivity towards IX, being proposed as a method to enrich natural hop´s extracts in IX. On the other hand, the extracts thus obtained were chemically characterized and evaluated for their anti-inflammatory activity, which was higher than the expected by its content in IX. KEYWORDS: Prenylflavonoids, xanthohumol (XN), isoxanthohumol (IX), Pressurized HotACCEPTED Water Extraction (PHWE), MANUSCRIPT anti-inflamatory. -
By Intestinal Microbiota; Conversion of Isoxanthohumol Into 8-Prenylnaringenin
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Ghent University Academic Bibliography J. Agric. Food Chem. 2005, 53, 6281−6288 6281 Activation of Proestrogens from Hops (Humulus lupulus L.) by Intestinal Microbiota; Conversion of Isoxanthohumol into 8-Prenylnaringenin SAM POSSEMIERS,† ARNE HEYERICK,‡ VEERLE ROBBENS,† DENIS DE KEUKELEIRE,‡ AND WILLY VERSTRAETE*,† Laboratory of Microbial Ecology and Technology (LabMET), Faculty of Bioscience Engineering, Gent UniversitysUGent, Coupure Links 653, and Laboratory of Pharmacognosy and Phytochemistry, Faculty of Pharmaceutical Sciences, Gent UniversitysUGent, Harelbekestraat 72, B-9000, Gent, Belgium Hop, an essential ingredient in most beers, contains a number of prenylflavonoids, among which 8-prenylnaringenin (8-PN) would be the most potent phytoestrogen currently known. Although a number of health effects are attributed to these compounds, only a few reports are available about the bioavailability of prenylflavonoids and the transformation potency of the intestinal microbial community. To test these transformations, four fecal samples were incubated with xanthohumol, isoxanthohumol (IX), and 8-PN. Upon incubation with IX, present in strong ales up to 4 mg/L, 36% was converted into 8-PN in one fecal sample and the estrogenic properties of the sample drastically increased. In an experiment with 12 fecal cultures, this conversion was observed in one-third of the samples, indicating the importance of interindividual variability in the intestinal microbial community. Eubacterium limosum was identified to be capable of this conversion (O-demethylation) of IX into 8-PN, and after strain selection, a conversion efficiency of 90% was achieved. Finally, strain supplementation to a nonconverting fecal sample led to rapid and high 8-PN production at only 1% (v/v) addition. -
Sniffing out Evolution
WAGENINGEN NIFFING OUT VOLUTION UR S E Congruence of Fragrances and Phylogenetic Relationships in Annonaceae Jeike L. van de Poel BSc. – MBI 890421659010 Under supervision of Dr. Lars Chatrou and Dr. Kate Goodrich Biosystematics group, October 2012 Course code: BIS-80439 Abstract The flowering plants are a relatively young group that has reached high levels of diversity and harbors an enormous assortment of chemical components. Annonaceae encompass an astounding amount of different flower fragrances. Floral odor is important in the attraction of pollinators, especially if the pollinators in question are beetles, which is the case for most Annonaceae. A floral fragrance consists of approximately 100 Volatile Organic Compounds. From two genera within the Annonaceae (Asimina Adans. and Deeringothamnus Small) the complete chemical composition of the floral scent has been studied. These chemical data were provided in order to perform optimizations over a simplified version of an existing phylogenetic tree based on chloroplast markers (ITS, accD-psal, matK-trnK, psbA-trnH, psbM-ycf6, rpL16 intron, rpl32-trnl, rpoB-trnC, trnC-ycf6, trnL-trnL-trnF, trnS-psbC, trnS-trnfM, ycf1). Due to a lack of previous studies in which phylogenetic analysis and extensive collection of fragrance data are combined, there is no real agreement as to what is the best method to use fragrance data in phylogenetic analyses. The aim of this study is to find out which method (within Maximum Parsimony and Maximum Likelihood optimization) is best to use for optimization of components from floral fragrances, and whether it is possible to use chemical data as a means to resolve polytomies. -
Ethnomedicine and Materia Medica Used by Kui Traditional Healers in Northeast Thailand
ETHNOMEDICINE AND MATERIA MEDICA USED BY KUI TRADITIONAL HEALERS IN NORTHEAST THAILAND MISS ARIKA VIRAPONGSE A THESIS FOR THE DEGREE OF MASTER OF SCIENCE KHON KAEN UNIVERSITY 2006 ISBN 974-626-847-3 ETHNOMEDICINE AND MATERIA MEDICA USED BY KUI TRADITIONAL HEALERS IN NORTHEAST THAILAND MISS ARIKA VIRAPONGSE A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE IN PHARMACEUTICAL CHEMISTRY AND NATURAL PRODUCTS GRADUATE SCHOOL KHON KAEN UNIVERSITY 2006 ISBN 974-626-847-3 อารีกา วีระพงส. 2549. การแพทยพื้นบานและยาสมุนไพรที่ใชโดยหมอพื้นบานชนชาติกุยใน ภาคตะวันออกเฉียงเหนือของประเทศไทย. วิทยานิพนธปริญญาวิทยาศาสตรมหาบณฑั ิต สาขาวิชาเภสชเคมั ีและผลิตภัณฑธรรมชาติ บัณฑิตวิทยาลัย มหาวิทยาลัยขอนแกน. [ISBN 974-626-847-3] อาจารยที่ปรึกษาวิทยานิพนธ: รศ.ดร. ชยันต พิเชียรสุนทร, ผศ.ดร.จุลรัตน คนศิลป, อ. ประธาน ฦาชา บทคัดยอ ชนชาติกุยเปนกลุมชาติพันธุหนึ่งในหาสิบกลุมชาติพันธุที่ไมใชไตในประเทศไทย และอาจเปน กลุมชาติพันธุแรก ๆ ที่เขามาตั้งถิ่นฐานอาศัยอยูทางตอนใตของภาคตะวันออกเฉียงเหนือของประเทศไทย การศึกษานี้มีวัตถุประสงคเพื่อศึกษาประมวลแนวคิดในเชิงทฤษฎีในระบบการแพทยพื้นบานของกลุมชาติ พันธุกุย และยาสมุนไพรที่ใชบําบัดโรคหรือความเจ็บปวยตาง ๆ กลุมประชากรที่ใชในการศึกษาครั้งนี้เปน หมอพื้นบานชาวกุยจํานวน 388 คน ซึ่งตั้งถิ่นฐานอยูในพื้นที่จังหวัดสุรินทร ศรีสะเกษ และอุบลราชธานี โดยการศึกษาเชิงลึกกับหมอพื้นบานจํานวน 30 คน วิธีวิจัยที่ใชในการเก็บรวบรวมขอมูลประกอบดวยการ ใชความจําแบบเสรี บันทึกขอมูลดานสุขภาพ การสังเกตการณโดยนักวิจัย การสังเกตการณโดยผูเขารวม โครงการวิจัย การเก็บรวบรวมตัวอยางสมุนไพร และการสัมภาษณ ผลที่ไดไดวิเคราะหและประมวลเปน -
An Examination of Medicinal Ethnobotany and Biomedicine Use in Two Villages on the Phnom Kulen Plateau Taylor Walker Hollins University, [email protected]
Hollins University Hollins Digital Commons Undergraduate Research Awards Student Scholarship and Creative Works 4-26-2017 An examination of medicinal ethnobotany and biomedicine use in two villages on the Phnom Kulen plateau Taylor Walker Hollins University, [email protected] Follow this and additional works at: https://digitalcommons.hollins.edu/researchawards Part of the Medicine and Health Sciences Commons Recommended Citation Walker, Taylor, "An examination of medicinal ethnobotany and biomedicine use in two villages on the Phnom Kulen plateau" (2017). Undergraduate Research Awards. 36. https://digitalcommons.hollins.edu/researchawards/36 This Article is brought to you for free and open access by the Student Scholarship and Creative Works at Hollins Digital Commons. It has been accepted for inclusion in Undergraduate Research Awards by an authorized administrator of Hollins Digital Commons. For more information, please contact [email protected], [email protected]. An examination of medicinal ethnobotany and biomedicine use in two villages on the Phnom Kulen plateau Taylor J. Walker Hollins University Roanoke, VA, United States Center for Mekong Studies, The School for Field Studies Siem Reap, Cambodia Research Advisor: Lisa Arensen, Ph.D. 5 May 2016 Contents Figures...................................................................................................................................... iii Tables ......................................................................................................................................