CURRICULUM VITAE VIRGINIA P. EDGCOMB

Woods Hole Oceanographic Institution Current Position: Associate Scientist Department of Geology and Geophysics Tel: (508) 289-3734 220 McLean Lab, MS#8 Fax: (508) 457-2183 Woods Hole, MA 02543 e-mail: [email protected]

GRADUATE EDUCATION 1997 Ph.D. Biology, University of Delaware (J. H. McDonald and D. W. Smith). Thesis title: Molecular Analysis of Spatial Heterogeneity of Sulfate-Reducing Bacteria in a Delaware Salt Marsh.

RESEARCH EXPERIENCE/APPOINTMENTS 2014-Present Associate Scientist with Tenure, Woods Hole Oceanographic Institution. 2009–2014 Research Specialist, Woods Hole Oceanographic Institution. 2005–2009 Research Associate, Woods Hole Oceanographic Institution. 2002–2004 Visiting Investigator, Woods Hole Oceanographic Institution. 2000–2002 National Research Council Postdoctoral Associate at the Woods Hole Oceanographic Institution, Woods Hole, MA. Project focused on an assessment of the potential habitat range of selected hydrothermal vent hyperthermophillic archaea-based laboratory tests of their tolerance to various stressors typical of subsurface conditions. This project was funded by a NASA Astrobiology Institute/NRC postdoctoral associateship awarded to V.E. in 2000 (advisor: A. Teske, WHOI/UNC Chapel Hill). 1999–2000 Staff Scientist—The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, MA (with M.L. Sogin). 1998 University of Sydney, Australia. Visiting scholar, June-Sept. Protist isolation and cultivation from environmental samples, and electron microscopy (with D. Patterson). 1997–1999 Postdoctoral Fellow in The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution. Projects included; isolation of novel protists from extreme environments, expanding phylogenies of deep-branching based on small subunit rRNA and protein coding genes, examining diversity of prokaryotic and eukaryotic microorganisms in extreme environments, particularly hydrothermal vent and other anaerobic freshwater and marine sediments using molecular and culture-based approaches (with M.L. Sogin). 1997 Marine Biological Laboratory, Woods Hole, MA Workshop on Molecular Evolution. 1997 United States Environmental Protection Agency Microbial Ecology Branch, Gulf Breeze, Florida. Visiting scholar working on molecular microbial ecology.

AWARDS/FELLOWSHIPS 2012 Seymour H. Hutner Prize in Protistology (awarded by International Society of Protistologists) 2001 NASA/NRC Astrobiology Institute Postdoctoral Associateship Award (admin. by the NRC renewal). 2000 NASA/NRC Astrobiology Institute Postdoctoral Associateship Award (admin. by the NRC). V. EDGCOMB – 1 1999 Award from Gordon Research Conferences to attend the 1999 Gordon Conference on Molecular Evolution in Hayama, Japan. 1997 Block Fellowship. A competitive fellowship awarded to a Biology Department graduate student in recognition of outstanding achievement, University of Delaware.

TEACHING EXPERIENCE 2002-present Part-time Life Science Teacher, Falmouth Academy, Falmouth, MA. 2003–2008 Lecturer and laboratory instructor for “Living in the Microbial World” secondary science teacher training workshops (NASA) Marine Biological Laboratory, Woods Hole, MA. 1998 Course Assistant, Workshop on Molecular Evolution, Marine Biological Laboratory, Woods Hole, MA. 1997 Course Instructor, University of Delaware-Microbiology. 1992–1997 Graduate teaching assistant, Department of Biology, University of Delaware.

PROFESSIONAL AFFILIATIONS • American Society of Microbiologists • International Society of Protistologists • American Society for Limnology and Oceanography • International Society for Microbial Ecology

PROFESSIONAL ACTIVITIES • President-elect 2014 International Society of Protistologists • Full Member: Science Scientific Committee on Oceanic Research (SCOR) Working Group on Microbial Community Responses to Ocean Deoxygenation (2014-2019) • EarthCube End User Workshop for Deep-Sea Processes and Dynamics, URI Graduate School of Oceanography, Narragansett, RI, June, 2013. • Moore Foundation Workshop on Microbial Ecology and Biogeochemistry of Oxygen-Deficient Marine Waters (OMZ) in Santa Cruz, Chile, 3/2013. • Associate Editor, Frontiers in Extreme Microbiology (2010-present) • Review Editor, Frontiers in Microbial Symbioses (2014-present) • Review Editor, Frontiers in Marine Molecular Biology and Ecology (2014-present) • Special Topics Editor, Deep Subsurface Microbiology, Frontiers in Extreme Microbiology (2012) • Editorial Advisor of New England Science Public Series on Evolution. • Special Topics Editor, Eukaryotes in Extreme Environments, Frontiers in Microbiology (2012) • Co-Chair of Special Session at Aquatic Sciences Meeting, New Orleans - Microbial Ecology of Hypersaline Habitats 2012 • Assoc. member SCOR Working Group 134 — Microbial Carbon Pump in the Ocean (2012) • Development of a WEB-based resource for COSEE NOW (Centers for Ocean Sciences Education Excellence, Networked Ocean World) to help scientists to prepare more effective Broader Impact Statements for NSF Criterion II, January, 2012 • NSF Panel on Shipboard Technical Services, February, 2012 V. EDGCOMB – 2 • Annual 2-day summer workshop on Marine Biotechnology for high school students, through the Weston High School Advanced Biotechnology Institute (2010 to present) • Initiated trimester internship program between Falmouth Academy high school students and WHOI science laboratories — first program year 2011-2012 • Participant in IODP “Building U.S. Strategies for 2013-2023 Scientific Ocean Drilling” workshop, Denver, CO 5/2012 • Dive and Discover Expeditions to the Seafloor — Expedition 14 (2011) • Chair, Nominating Committee, ISOP 2010-11 • Chair of Special Session at ASLO 2011, San Juan Puerto Rico — Protists in Extreme Environments: Beyond Diversity. • Session Leader C-DEBI RCN, Microbial activity, physiology, metabolism 2011 • Board of Directors, Zephyr Education Foundation 2010 to present • Education Advisory Committee for Waquoit Bay National Estuarine Research Reserve (2011 to present) • 2012-2015 WHOI Partnership Program • NSF Panel on Oceanographic Instrumentation/Shipboard Scientific Support Equipment, January 2010. • WHOI Women’s Committee (2010 to 2013)

OTHER PROFESSIONAL ACTIVITIES: MANUSCRIPT/PROPOSAL REVIEWER Nature Geosciences National Science Foundation Journal of Eukaryotic Microbiology Limnology and Oceanography Molecular Biology and Evolution NASA Ecology Extremophiles Aquatic Microbial Ecology Environmental Microbiology Applied and Environmental Microbiology Protist Trends in Ecology and Evolution ISME Journal PNAS Microbial Ecology Frontiers FEMS Microbiology Letters Molecular Ecology Resources FEMS Microbial Ecology

NEW TECHNOLOGY DEVELOPMENT Development of new technology for in situ studies in marine microbial ecology, the Microbial Sampler - Submersible Incubation Device, MS-SID) together with Craig Taylor (WHOI Biology) and McLane Research Laboratories, E. Falmouth, MA. Retrofitting and application of an older technology (Deep-SID) toin situ fixation of water samples for microscopy in Cariaco Basin with Craig Taylor (WHOI Biology).

GROUP MEMBERS AND GUESTS CURRENT • Postdoc, Maria Pachiadaki, Oct. 2012 to present.

V. EDGCOMB – 3 • Postdoc, Roxanne Beinart, Dec. 2013 to present. • Research Associate, David Beaudoin, assists on various research projects, 2006–present. • Dr. Sofia Ibarraran • Thesis committee member for PhD candidates Joseph Russell (U. Delaware, 2011-present), Elizabeth Suter (Stony Brook University 2013-present)

GROUP MEMBERS AND GUESTS PAST • Dr. Konstantinos Kormas, sabbatical visitor 2013 • Postdoc, William T. Orsi, 2011– 2013. • PhD candidate Vanessa Redou, Laboratoire de Biodiversite et Ecologie Microbienne, ESMISAB, France, 2/2014-5/2014) and PhD committee member. • Masters candidate Manon Duret, Universite du Littoral, CNRS-UMR, France (1/2014-6/2014) • PhD candidate Emma Louise Rocke, Hong Kong University of Science and Technology (6/2013- 12/2013-6/2013) and PhD committee member. • Joseph Russell, U. Delaware, in my laboratory 3/2012, Ph.D. committee member 2011-present. • Sophie Charvet, U. Laval, Canada, as Guest Graduate Student in my laboratory spring 2012. • Verena Salman, Max-Planck, Germany as Guest Investigator in my laboratory 8/1/2012–9/30/2012. • Gaëtan Burgaud, U. Brest, France as Guest Investigator 1/2011–5/2011. • Dr. Ankita Srivastava, 2013-14. • Summer Undergraduate Guest Students: Vincent Klokman from Univ. North Carolina, summer 2009; Anja Hickethier, Kaiserslautern University, Germany, spring 2010; Greg Gotta, St. Lawrence University, summer 2010; Brecia Douglas, Northeastern U., summer 2011; Colin Morrison, U. Nevada Reno (co-advised with J. Bernhard), summer 2012. • MS candidate Aimee Gillespie, MIT. Committee member and supervisor of her molecular microbial ecology work, 2011–2012. • PhD candidate William Orsi, Northeastern University. PhD committee member and co-supervisor with Slava Epstein (Northeastern U., his primary thesis advisor) of the environmental sequencing portion of his thesis project (2007-2011), and supervisor of his work on the E. Mediterranean “Pickled Protists” project, 2007–2011. • Undergraduate Research Fellows Simone Boer and Karen Lloyd, while working in Teske Lab on pressure, sulfide, heavy metal, and pH tolerance of deep-sea hyperthermophiles 2000-2002. • Technician, David Kysela, working on molecular survey of prokaryotic and eukaryotic diversity in Guaymas Basin hydrothermal sediments, 1998–2000. • Numerous high school students on short-term research projects, 1997-present.

CRUISE PARTICIPATION • Co-Chief Scientist cruise to Cariaco Basin (Venezuela), R/V Hermano Gines, water column meta- omics study of eukaryotic activities along redoxcline (3 days — November 2014) • Co-Chief Scientist cruise to Cariaco Basin (Venezuela), R/V Hermano Gines, water column meta- omics study of eukaryotic activities along redoxcline (3 days — May 2014) • Participating Scientist cruise to E. Mediterranean, R/V Urania, microbiology of deep hypersaline brine pools (19 days — September 2012)

V. EDGCOMB – 4 • Chief Scientist cruise to Santa Barbara Basin (CA), R/V Robert Gordon Sproul, symbiosis study (4 days-January 2012) • Chief Scientist cruise to E. Mediterranean, R/V Atlantis, microbiology of deep hypersaline brine pools (14 days — November 2011) • Participating Scientist cruise to Santa Barbara Basin (CA), R/V Robert Gordon Sproul, symbiosis study and denitrification study (6 days — April 2010) • Participating Scientist cruise to Bahamas, R/V Walton Smith Bahamian stromatolite eukaryotes (8 days—March 2010) • Chief Scientist cruise to E. Mediterranean, R/V Oceanus, microbiology of deep hypersaline brine pools (8 days — July 2009) • Chief Scientist cruise to Cariaco Basin (Venezuela) R/V Hermano Gines, water column microbial diversity study (4 days — Nov 2009) • Chief Scientist cruise to Santa Barbara Basin (CA), R/V Robert Gordon Sproul, symbiosis study and denitrification study (5 days — June 2009) • Chief Scientist cruise to Santa Barbara Basin (CA), R/V Robert Gordon Sproul, symbiosis study and denitrification study (7 days — Oct 2008) • Participating Scientist cruise to Santa Barbara Basin (CA), R/V Robert Gordon Sproul, symbiosis study and denitrification study (4 days — June 2008) • Participating Scientist cruise to Santa Barbara Basin (CA), R/V Robert Gordon Sproul, symbiosis study and denitrification study (6 days — Oct 2007) • Chief Scientist cruise to Cariaco Basin (Venezuela) R/V Hermano Gines, water column microbial diversity study (5 days — Nov 2007) • Chief Scientist cruise to Cariaco Basin (Venezuela) R/V Hermano Gines, water column microbial diversity study (5 days — Jan 2005) • Chief Scientist cruise to Cariaco Basin (Venezuela) R/V Hermano Gines, water column microbial diversity study (5 days — May 2005) • Participating Scientist on a joint NOAA/National Geographic cruise to Stellwagen Bank addressing microbial diversity (2 days-July 1998) • Participating Scientist cruise to Guaymas Basin, R/V Atlantis/Alvin, deep-sea thermal vent sediment sampling (17 days — May 1997)

RECENT INVITED TALKS (WITHIN LAST THREE YEARS) 2015 ASLO/Ocean Sciences Meeting, Granada, Spain, (2/2015) 2015 Falkland Islands Science Symposium hosted by Falkland Islands Government and the British Embassy, Falkland Islands, Jan. 2015. 2014 University of Rhode Island Seminar Series, Sept. 19, 2014. 2014 NorthEastern Microbiologists: Physiology, Ecology and (NEMPET) Keynote Address, Blue Mtn. Lake, NY (6/2014) 2014 ASLO/Ocean Sciences Meeting, Honolulu, Hawaii, Symposium Speaker 2013 New England Science Public 7th Biology New England South 2013 meeting, Bristol, RI Keynote Address (12/2013) 2013 McLane Research Laboratories, Falmouth, MA (12/2013) 2013 Hong Kong University of Science and Technology, Hong Kong (12/2013)

V. EDGCOMB – 5 2013 Symposium speaker, Ciliates and the Rare Biosphere Session, International Congress of Protistology XIV (July 2013, Vancouver, Canada) 2013 University of North Carolina, Chapel Hill (4/2013) 2012 Massachusetts Institute of Technology (11/2012) 2012 University of Massachusetts Dartmouth (11/2012) 2012 University of Tennessee (10/2012) 2012 International Council for Science, Scientific Committee on Oceanic Research (SCOR Working Group 134 -Microbial Carbon Pump in the Ocean (8/12, Delmenhorst, Germany) 2012 Symposium speaker Protist 2012 (joint meeting between International Society of Protistologists (ISOP) and International Society for Evolutionary Protistology (ISEP) — Field Protistology: Cornerstone for understanding the diversity, ecology and evolution of microbial eukaryotes — Oslo Norway (7/2012) 2012 Seymour H. Hutner Keynote Lecture, Joint meeting between International Society of Protistologists (ISOP) and International Society for Evolutionary Protistology (ISEP) Oslo, Norway (7/2012) 2012 International Society for Microbial Ecology (ISME) — Session on: Microbial Ecology in Extreme Environments - Copenhagen, Denmark (8/2012) 2012 University of British Columbia, Victoria Experimental Network Under the Sea (VENUS) Symposium on Saanich Inlet, Victoria, BC, Canada (5/2012) 2012 University of Maryland Center for Environmental Science, Horn Point Laboratory (4/2012) 2012 Keynote Speaker, Massachusetts Association of Biology Teachers Annual Meeting, Framingham State University (3/2012)

PUBLICATIONS (refereed, *Postdoc or Student Advisee, #Technical Staff Advisee) *Rocke, E., Pachiadaki, M.G., Cobban, A., Kujawinski, E.B., Edgcomb, V.P. 2015. Protist community grazing in deep ocean waters. In press, PLoS ONE. Taylor, C.D., Edgcomb, V.P., Doherty, K.W., Engstrom, I., Shanahan, T., Pachiadaki, M.G., Molyneaux, S.J., and Honjo, S. 2015. Fixation filter, device for the rapid in situ preservation of particulate samples. Deep Sea Research I, 96:69-79. Gong, J., Shi, F., Ma, B., Dong, J., Pachiadaki, M., Zhang, X., Edgcomb, V.P. 2015. Depth shapes alpha- and beta-diversities of microbial eukaryotes in surficial sediments of coastal ecosystems. Environmental Microbiology Parris, D.J., Ganesh, S., Edgcomb V., DeLong, E.F., Stewart, F.J. 2014. Microbial diversity in the marine oxygen minimum zone off northern Chile. Frontiers in Microbiol, Aquatic Microbiology, doi: 10.3389/fmicb.2014.00543. Bernhard, J.M., Kormas, K.A., Pachiadaki, M.G., Rocke, E., Beaudoin, D.J., Morrison, C., Visscher, P.T., Cobban, A., Starczak, V.R., and Edgcomb, V.P. 2014. Benthic protists and fungi of Mediterranean deep hypersaline anoxic basin redoxcline sediments. Frontiers in Extreme Microbiology, doi: 10.3389/fmicb.2014.00605. *Redou, V., Ciobanu, M.C., Pachiadaki, M.G., Edgcomb, V., Alain, K., Barbier, G., and Burgaud G. 2015. In-depth analyses of deep subsurface sediments using 454-pyrosequencing reveals a reservoir of buried fungal communities. FEMS Microbiol Ecol, doi:10.1111/1574-6941.12447. *Pachiadaki, M.G., Yakimov, M.M., Leadbetter, E., Edgcomb, V.P. 2014. Unveiling microbial activities along the halocline of Thetis, a deep hypersaline anoxic basin in the Eastern Mediterranean Sea. The ISME J. doi:10.1038/ismej.2014.100. V. EDGCOMB – 6 Stoeck, T., Filker, S., Edgcomb, V., Orsi, W., Yakimov, M.M., Pachiadaki, M., Breiner, H.-W., LaCono, V., and Stock, A. 2014. Living at the limits: Evidence for microbial eukaryotes thriving under pressure in deep anoxic, hypersaline habitats. Advances in Ecology vol. 2014, Article ID 532687, doi:10.1155/2014/532687. Honjo, S., Eglinton, T.I., Taylor, C.D., Edgcomb, V., Iglesias-Rodrigues, D.M., Krishfield, R.A., van Mooy, B., Repeta, D.J., Sievert, S.M., Ulmer, K.M., and members of the GBF Scientific Steering Committee. 2014. A biogeochemical flux observatory initiative to understand the ocean’s role in global cycling of bioactive carbon. Oceanography 27(3):10-16, doi: 10.5670/oceanog.2014.78. *Pachiadaki, M., Taylor, C., Oikomomou, A., Yakimov, M., Stoeck, T., Edgcomb, V.P. 2014. In situ grazing experiments apply new technology to gain insights into deep sea food webs. Deep Sea Res II, doi:10.1016/j.dsr2.2014.10.019. Edgcomb, V.P., Taylor, C., Pachiadaki, M., Engstrom, I., Yakimov, M. 2014. Comparison of Niskin vs. in situ approaches for analysis of gene expression in deep Mediterranean Sea water samples. Deep Sea Res II, doi: 10.1016/j.dsr2.2014.10.020. Edgcomb, V.P., Pachiadaki, M. 2014. Ciliates along oxyclines of permanently stratified marine water columns. J. Euk. Microbiol. 61:434-445. #Beaudoin, D.J., Carmichael, C.A., Nelson, R.K., Reddy, C.M., Teske, A., Edgcomb, V.P. 2014. Impact of protists on a hydrocarbon degrading bacterial community from deep-sea Gulf of Mexico sediments. Deep-Sea Research II, doi:10.1016/j.dsr2.2014.01.007. Edgcomb, V.P., Orsi, W., Biddle, J.F. 2014. Fungi in the marine subsurface. In: Life in Extreme Environments: Microbial Life in the Deep Biosphere, J. Kallmeyer ed., DeGruyter Publishing, pp. 187-197. Teske, A., Biddle, J.F., Edgcomb, V.P., Schippers, A. 2013. Deep subsurface microbiology: a guide to the research topic papers. Frontiers in Microbiology doi: 10.3389/fmicb2013.00122. Heger, T.J., Edgcomb, V.P., Kim, E., Lukes, J., Leander, B.S., Yubuki, N. 2013. A resurgence in field research is essential to better understand diversity, ecology and evolution of microbial eukaryotes. In press, J. Euk. Microbiol. Grabenstatter, J., Mehay, S., McIntyre-Wressnig, A., Giner, J.-L., Edgcomb, V.P., Beaudoin, D., Bernhard, J., Summons, R.E. 2013, Identification of 24-n-propylidenecholesterol in a member of the Foraminifera. Organic Geochemistry 63:145-151. Edgcomb, V.P., Bernhard, J.M., Summons, R.E., Orsi, W., Beaudoin, D., Visscher, P. 2013, Active eukaryotes in microbialites of Highborne Cay, Bahamas and Shark Bay, Australia. ISME Journal, doi: 10.1038/ismej.2013.130. Stock, A., Edgcomb, V.P., Orsi, W., Filker, S., Breiner, H.W., Yakimov, M.M., Stoeck, T. 2013, Evidence for isolated evolution of deep-sea ciliate communities through environmental selection and geological chronology. BMC Microbiology 13:150 doi:10.1186/1471-2180-13-150. Burgaud, G., Woehlke, S., Redou, V., Orsi, W., Beaudoin, D., Barbier, G., Biddle, J.F., Edgcomb, V.P. 2013, Deciphering presence and activity of fungal communities in marine sediments using a model estuarine system. Aquatic Microbial Ecol 70:45-62. Bernhard, J.M., Edgcomb, V.P., McIntyre-Wressnig, A., Summons, R.E., Visscher, P.T., Jeglinski, M. 2013, Insights into foraminiferal influences on microfabrics of microbialites at Highborne Cay, Bahamas. PNAS doi:10.1073/pnas.1221721110. *Orsi, W., Edgcomb, V.P., Christman, G.D., and Biddle, J.F. 2013, Gene expression in the deep biosphere. Nature doi:10.1038/nature12230. *Orsi, W. and Edgcomb, V.P. 2013, Microbial eukaryotes in marine oxygen minimum zones. In: Seckbach, J., Oren, A., and Stan-Lotter, H. (eds.), COLE Series: Polyextremophiles – Life Under Multiple Forms of Stress, Cellular Origin, Life in Extreme Habitats and Astrobiology, Vol. 27, V. EDGCOMB – 7 Springer Verlag, 634pp. Edgcomb, V.P. and Orsi, W. 2013, Microbial eukaryotes in hypersaline anoxic deep sea basins. In: Seckbach, J., Oren, A., and Stan-Lotter, H. (eds.), COLE Series: Polyextremophiles – Life Under Multiple Forms of Stress, Cellular Origin, Life in Extreme Habitats and Astrobiology, Vol. 27, Springer Verlag, 634pp. Edgcomb, V.P., Bernhard JM. 2013, Heterotrophic protists in hypersaline microbial mats and deep hypersaline basin water columns. Special Issue “Extremophiles and Extreme Environments” Life doi:10.3390/life3020346. *Orsi, W., Biddle, J., and Edgcomb, V.P. 2013, Deep sequencing of subseafloor eukaryotic rRNA reveals active fungi across marine subsurface provinces. PLoS ONE, 8(2): e56335. doi:10.1371/ journal.pone.0056335 Edgcomb, V.P., R.E. Summons, J.M. Bernhard, D. Beaudoin, S. Pruss, A. Gillespie. 2013, Microbial diversity in oolitic sands of Highborne Cay, Bahamas. Geobiology doi: 10.1111/gbi.12029. Filker, S., Stock, A., Breiner, H.W., Edgcomb, V.P., Orsi, W., Yakimov, M.M., and Stoeck, T. 2012, Environmental selection of protistan communities in hypersaline anoxic deep-sea basins, Eastern Mediterranean Sea. MicrobiologyOpen 2(1):54-63. *Orsi, W., S. Charvet, J. Bernhard, and Edgcomb, V.P. 2012, Prevalence of partnerships between bacteria and ciliates in oxygen-depleted marine water columns. Frontiers in Extreme Microbiol.3:341. doi: 10.3389/fmicb.2012.00341. Bernhard, J.M., Casciotti, K.L., McIlvin, M.R., Beaudoin, D.J., Visscher, P.T., and Edgcomb, V.P. 2012, Potential importance of physiologically diverse benthic foraminifera in sedimentary nitrate storage and respiration. JGR Biogeosciences 117, G03002, doi:10.1029/2012JG001949. *Orsi, W., Y.C. Song, S. Hallam, and Edgcomb, V.P. 2012, Effect of oxygen minimum zone formation on communities of marine protists. ISME Journal, doi: 10.1038/ismej.2012.7 Beaudoin, D.J., J.M. Bernhard, and Edgcomb, V.P. 2012, A novel ciliate (Ciliophora: Hypotrichida) isolated from bathyal anoxic sediments. In: A. Altenbach, J. Bernhard, and J. Seckbach (eds.) Anoxia: Paleontological Strategies and Evidence for Eukaryote Survival Springer-Verlag. 147-152. *Orsi, W., Edgcomb, V.P., Faria, J., Foissner, W., Fowle, W.H., Hohnmann, T., Suarez, P., Taylor, C., Taylor, G.T., Vd’acny, P., and Epstein, S. 2012, Class Cariacotrichea, a novel ciliate taxon from the anoxic Cariaco Basin, Venezuela. The International Journal of Systematic and Evolutionary Microbiology doi:10.1099/ijs.0.034710-0. Bernhard, J.M., Edgcomb, V.P., Casciotti, K.L., McIlvin, M.R., and Beaudoin, D.J. 2011, Denitrification likely catalyzed by endobionts in an allogromiid foraminifer. ISME Journal 6(5):951-60. Stock, A., Breiner, H.-W., Pachiadaki, M., Edgcomb, V.P., Filker, S., LaCono, V., Yakimov, M., Stoeck, T. 2011, Microbial eukaryote life in the new hypersaline deep-sea basin Thetis. Extremophiles 16(1):21-34. Edgcomb, V.P., W. Orsi, H.-W. Breiner, A. Stock, S. Filker, M.M. Yakimov, and T. Stoeck. 2011, Novel kinetoplastids associated with hypersaline anoxic lakes in the Eastern Mediterranean deep-sea. Deep-Sea Research I 58(10):1040-1048. Edgcomb, V.P., Leadbetter, E.R., Bourland, W., Beaudoin, D., and Bernhard, J.M. 2011, Structured multiple endosymbiosis of bacteria and archaea in a ciliate from marine sediments: A survival mechanism in low oxygen, sulfidic sediments? Frontiers in Microbial Physiology and Metabolism 2: doi: 10.3389/fmicb.2011.00055. Edgcomb, V.P., Orsi, W., Bunge, J., Jeon, S.-O., Christen, R., Leslin, C., Holder, M., Taylor, G.T., Suarez, P., Varela, R., and Epstein, S. 2011, Protistan microbial observatory in the Cariaco Basin, Caribbean. I. Pyrosequencing vs. Sanger insights into species richness. ISME J 5:1344-1356.

V. EDGCOMB – 8 Edgcomb, V.P., Orsi, W., Taylor, G.T., Vdacny, P., Taylor, C., Suarez, P., and Epstein, S. 2011, Accessing marine protists from the anoxic Cariaco Basin. ISME J. 5:1237-1241. *Orsi, W., Edgcomb, V.P., Jeon, S.O., Leslin, C., Bunge, J., Taylor, G.T., Varela, R, and S. Epstein. 2011, Protistan microbial observatory in the Cariaco Basin, Caribbean. II. Habitat specialization. ISME J 5:1357-1373. Edgcomb, V.P., and Biddle, J. 2011, Microbial eukaryotes in the marine subsurface? In: A. Altenbach, J. Bernhard, and J. Seckbach (eds.) Anoxia: Paleontological Strategies and Evidence for Eukaryote Survival Springer-Verlag 479-491. Edgcomb, V.P., and Stoeck, T. 2011, Massively parallel tag sequencing unveils the complexity of marine protistan communities in oxygen-depleted habitats. In: M. Harbers and G. Kahl (eds.) Tag-based Approaches for Next Generation Sequencing, Wiley-Blackwell-VCH, Weinheim. *Dilegge, S.K., Edgcomb, V.P., and Leadbetter, E.R. 2011, Presence of the oral bacterium Capnocytophaga canimorsus in the tooth plaque of canines. Vet. Microbiol. 149(3-4):437-45. Edgcomb, V.P., Beaudoin, D., Gast, R., Biddle, J., and Teske, A. 2010, Marine subsurface eukaryotes: the fungal majority. Environ. Microbiol. 13(1):172-183. Edgcomb, V.P., Breglia, S.A., Yubuki, N., Beaudoin, D., Patterson, D.J., Leander, B.S., and Bernhard, J.M. 2010, Identity of epibiotic bacteria on symbiontid euglenozoans in O2-depleted marine sediments: evidence for symbiont and host co-evolution. ISME J. 5:231-243 Teske, A., Edgcomb, V.P., Rivers, A., Thompson, J.R., Gomez, A.D.V., Molyneaux, S.J., and Wirsen, C.O., 2009, Toward a molecular and physiological survey of the Jannasch lab Thermococcus and Pyrococcus culture collection, Extremophiles 13(1):151-167. Stoeck, T.A., Behnke, A., Christen, R., Amaral-Zettler, L., Rodrigues-Mora, M.J., Chisterserdov, A., Orsi, W., and Edgcomb, V., 2009, Massively parallel tag sequencing reveals the complexity of anaerobic marine protistan communities, BMC Biology 7:72 doi:10.1186/1741-7007-7-72. Stoeck, T., and Edgcomb, V., 2009, Role of protists in microbial interactions with hydrocarbons, In: K. N. Timmis (ed.) Microbiology of Hydrocarbons, Oils, Lipids, and Derived Compounds, Springer-Verlag. Behnke, A., Engel, M, Edgcomb, V., and Stoeck, T., 2009, Studying the in situ role of protistan communities in hydrocarbon contaminated water samples via community profiling and CARD-FISH, In: K. N. Timmis (ed.) Microbiology of Hydrocarbons, Oils, Lipids, and Derived Compounds, Springer-Verlag. Fernandez-Delgado, M., Garcia-Amado, M.A., Contreras, M., Edgcomb, V., Vitelli, J., Gueneau, P., and Suarez, P., 2009, Vibrio cholerae non-01, non-0139 associated with seawater and plankton from coastal marine areas of the Caribbean Sea, Journal of Environmental Health Research, 19(4):279-289. Santelli, C.M., Bach, W., Edgcomb, V., and Edwards, K.J., 2009, The diversity and abundance of bacteria inhabiting seafloor lavas positively correlate with rock alteration,Environmental Microbiology, 11(1):86-98. Yubuki, N., Edgcomb, V.P., Bernhard, J.M., and Leander, B.S., 2009, Cellular identity of a diverse group of deep sea, anoxic euglenozoans with epibiotic bacteria: Ultrastructure and molecular phylogeny of Calkinsia aureus from the Santa Barbara Basin (California, USA), BMC Microbiology, 9:16. Edgcomb, V., Orsi, W., Leslin, C., Epstein, S.S., Bunge, J., Jeon, S., Yakimov, M.M., Behnke, A., and Stoeck, T., 2009, Protistan community patterns within the brine and halocline of deep hypersaline anoxic basins (DHABs) in the eastern Mediterranean Sea, Extremophiles 13(1):151- 167. Edgcomb, V.P., Molyneaux, S.J., Böer, S., Wirsen, C.O., Saito, M., Atkins, M., and A. Teske, 2007, Survival and growth of two heterotrophic hydrothermal vent archaea, Pyrococcus strain GB-D and Thermococcus fumicolans, under hydrothermal vent and subsurface stress factors, V. EDGCOMB – 9 Extremophiles, 11(2):329-342. Edgcomb, V.P., Bernhard, J.M., and Jeon, S., 2007, Deep-sea microbial eukaryotes in anoxic, microoxic, and sulfidic environments, In: J. Seckbach (ed.)Algae and Cyanobacteria in Extreme Environments. Series: Cellular Origin, Life in Extreme Habitats and Astrobiology, Vol. 11., Springer-Verlag. Dufernez, F., Yernaux, C., Gerbod, D., Noël, C., Chauvenet , M., Wintjens, R., Edgcomb, V., Capron, M., Opperdoes, F., and Viscogliosi, E., 2006, The presence of four iron-containing superoxide dismutase isozymes in Trypanosomatidae: characterization and subcellular localization in brucei, Free Radical Biology and Medicine, 40:210-225. *Lloyd, K.G., Molyneaux, S.J., Edgcomb, V.P., Wirsen, C.O., Atkins, M., and Teske, A.P., 2005, Growth and Survival of the hyperthermophilic chemolithoautotrophic archaea Methanocaldococcus jannaschii and Archaeoglobus profundus under high temperature, high sulfide concentrations and low pH, Applied and Environmental Microbiology, 71:6383-6387. Noel, C., Dufernez, F., Gerbod, D., Edgcomb, V., Delgado-Viscogliosi, P., Ho, L.-C., Singh, M., Wintjens, R., Sogin, M., Capron, M., Zenner, L., and Viscogliosi, E., 2004, Molecular phylogenies of Blastocystis isolates from different hosts: implicataions for genetic diversity, speciation, and zoonosis, Journal of Clinical Microbiology, 43(1):348-355. Edgcomb, V.P., Molyneaux, S., Boer, S., Lloyd, K., Saito, M., Erickson, J., Atkins, M., Wirsen, C., and Teske, A., 2004, Sulfide Ameliorates metal toxicity for hydrothermal vent archaea,Applied and Environmental Microbiology, 70(4):2551-2555. Kormas, K. Ar., Smith, D. C., Edgcomb, V., and Teske, A., 2003, Molecular analysis of deep subsurface microbial communities in Nankai Trough sediments (ODP Leg 190, Site 1176)., FEMS Micrbiology Letters, 45(2):115-125. Noël, C., Peyronnet, C., Gerbod, D., Edgcomb, V.P., Delgado-Viscogliosi, P., Sogin, M.L., Capron, M., Viscogliosi, E., and Zenner, L., 2003, Phylogenetic analysis of Blastocystis isolates from different hosts based on the comparison of small-subunit rRNA gene sequences, Molecular and Biochemical Parasitology, 4063:1-5. Gerbod,, D., Noel, C., Dolan, M.F., Edgcomb, V.P., Kitade, O., Noda, S., Dufernez, F., Ohkuma, M., Kudo, T., Capron, M., Sogin, M., and Viscogliosi, E., 2002, Molecular phylogeny of inferred from small subunit rRNA sequences, with emphasis on the Descovinidae and Calonymphidae (Trichomonadea), Molecular Phylogenetics and Evolution, 25(3):545-56. Simpson, A.G.B., Roger, A.J., Silberman, J.D., Leipe, D., Edgcomb, V., Jermiin, L., Patterson, D.J., and Sogin, M.L., 2002, Evolutionary history of “early-diverging” eukaryotes: the excavate taxon is a close relative of , Molecular Biology and Evolution, 19:1782-1791. Edgcomb, V.P., Kysela, D.T., Teske, A., Gomez, A.D.V., and Sogin, M.L., 2002, Benthic eukaryotic diversity in the Guaymas Basin hydrothermal vent environment, PNAS, 99(11):7663-7668. Edgcomb, V.P., Simpson, A.G.B., Amaral Zettler, L., Nerad, T.A., Patterson, D.J., and Sogin, M.L., 2002, Pelobionts are degenerate protists: Insights from molecules and morphology, Molecular Biology and Evolution, 19:978-982. Teske, A., Hinrichs, K.-U., Edgcomb, V., Gomez, A.D.V., Kysela, D., Sogin, M.L., and Jannasch, H.W., 2002, Archaeal and bacterial population structure of hydrothermal sediments at the Guaymas Basin vent sites: evidence for anaerobic methanotrophy, Applied and Environmental Microbiology, 68(4):1994-2007. Patterson, D., Sogin, M., Edgcomb, V., Amaral-Zettler, L. and McArthur, A., 2001, WEB-based resources for promoting awareness of microbial biodiversity [Online]. Website: http://mbl.edu/microscope Gerbod, D., Edgcomb, V.P., Noël, C., Vanacova, S., Wintjens, R., Tachezy, J., Sogin, M.L., and Viscogliosi, E., 2001, Phylogenetic relationships of fumarase genes from trichomonad species, Molecular Biology and Evolution 18(8):1574-1584.

V. EDGCOMB – 10 Gerbod, D., Edgcomb, V.P., Noël, C., Zenner, L., Wintjens, R., Delgado-Viscogliosi, P., Holder, M.E., Sogin, M.L., and Viscogliosi, E., 2001, Phylogenetic identification of the trichomonad parasite of turkeys, (Smith) Tyzzer, inferred from small subunit rRNA sequence, Journal of Eukaryotic Microbiology, 48(4):498-504. Walker, G., Simpson, A.G.B., Edgcomb, V.P., Sogin, M.L., and Patterson, D.J., 2001, Ultrastructural identities of Mastigamoeba punctachora, Mastigamoeba simplex, and Mastigella commutans and assessment of hypotheses of relatedness of the pelobionts (Protista), European Journal of Protistology, 37: 25-49. Edgcomb, V.P., Roger, A., Kysela, D., Simpson, A.G.B., Silberman, J. and Sogin, M.L., 2001, New insights into the phylogeny of eukaryotes based on alpha and beta tubulin gene sequences: emphasis on the flagellates, Molecular Biology and Evolution, 18(4):514-522. Gerbod, D., Edgcomb, V.P., Noël, C., Delgado-Viscogliosi, P., and Viscogliosi, E., 2000, Phylogenetic position of symbionts from the termite Kalotermes flavicollisbased on small subunit rRNA sequences, International Microbiology, 3:165-172. Delgado-Viscogliosi, P., Viscogliosi, E., Gerbod, D., Kulda, J., Sogin, M.L., and Edgcomb, V.P., 2000, Molecular phylogeny of parabasalids based on small subunit rRNA sequences, with emphasis on the Trichomonadinae subfamily, Journal of Eukaryotic Microbiology, 47(1):70-75. Edgcomb, V.P., McDonald, J.H., Devereux, R., and Smith, D.W., 1999, Estimation of bacterial cell numbers in humic-rich salt marsh sediments using probes to 16S rDNA, Applied and Environmental Microbiology, 65(4):1516-1523. Viscogliosi, E., Edgcomb, V.P., Gerbod, D., Noël, C., and Delgado-Viscogliosi, P., 1999, Molecular evolution inferred from small subunit rRNA sequences: what does it tell us about phylogenetic relationships and taxonomy of the parabasalids? Parasite, 6:279-291. Edgcomb, V.P., Viscogliosi, E., Simpson, A.G.B., Delgado-Viscogliosi, P., Roger, A.J. and Sogin, M.L.,1998, New Insights into the phylogeny of Trichomonads inferred from small subunit rRNA sequences, Protist, 149:359-366. Edgcomb, V.P., McDonald, J.H., and Smith, D.W., 1996, Molecular analysis of spatial heterogeneity of sulfate-reducing bacteria in a Delaware salt marsh, Microbial & Comparative Genomics, 1(4):375.

PUBLICITY Highlighted in 2015 “Women in Oceanography: A Decade Later” http://www.tos.org/oceanography/ archive/27-4_supplement.html The Harshest Habitats on Earth, Astrobiology Magazine, Extreme Life News posted 9/24/13. WHOI Press Release, “Fungi Flourish Below the Seafloor” (March 1st, 2013) WHOI Press Release, “Deep Biosphere Harbors Active, Growing Communities of Microorganisms” (June 12th, 2013) Highlighted in National Geographic online report on Falkland Islands Symposium hosted by the British Embassy and the South Atlantic Environmental Research Institute (January, 2015): http://voices. nationalgeographic.com/2015/02/22/whats-living-under-these-elephant-seals/

ABSTRACTS (last 3 years, bold indicates presenter) Edgcomb, V.P.. Pachiadaki, M., Kormas K. Ar., Taylor, C., Bernhard, J.M. Microbial interactions and processes in deep hypersaline anoxic basin water columns and sediments. ASLO/Ocean Sciences Meeting, 2/2015, Granada, Spain. Pachiadaki, M., Suter, E., Taylor, C., Montes-Herrera, E., Taylor, G., Edgcomb, V. Polyphasic approach

V. EDGCOMB – 11 to microbial processes and interactions along a stable marine redoxcline. ASLO, Seville, Spain 2/2015. Suter, E.A., Montes-Herrera, E., Pachiadaki, M., Edgcomb, V.P, Taylor, G.T. Describing nitrogen loss from the Cariaco Basin water column using both rate measurements and molecular approaches. ASLO, Seville, Spain, 2/2015. Edgcomb, V.P. Protistan denizens of marine oxyclines and their prokaryotic associates: big diners at submerged smorgasbords. NorthEastern Microbiologists: Physiology, Ecology and Taxonomy (NEMPET) Keynote Address, Blue Mtn. Lake, NY 6/2014. Kormas, K. Ar., M.G. Pachiadaki, H. Karayanni, E.R. Leadbetter, J.M. Bernhard, V. P. Edgcomb. Active prokaryotes in the sediments of three deep-sea Mediterranean hypersaline basins; General Meeting of the American Society of Microbiology Annual Meeting, Boston, MA, USA, 2914, abstract N-1325. Edgcomb, V.P., Pachiadaki, M.P., Kormas, K. Ar., Bernhard, J.M. Microbial Interactions and Processes in Deep Hypersaline Anoxic Basin Water Columns and Sediments. ASLO, Seville, Spain, 2/2015. Russell, J., Orsi, W., Edgcomb, V.P., Biddle, J.F. Microorganismal distribution in the Iberian Margin deep biosphere. Goldschmidt 2014, Sacramento, CA, 6/2014. Biddle, J.F., Orsi, W., Christman, G. Edgcomb, V. The first metatranscriptome from the deep subsurface. ASLO/Ocean Sciences Meeting Honolulu, Hawaii 2/2014. Bernhard, J.M., Edgcomb, V.P., et al. Redoxcline sediments of haloclines associated with Deep Hypersaline Anoxic Lakes support protist and fungal populations. Liege Colloquium, May 2014. Edgcomb, V.P., Pachiadaki, M.G., Kormas, K., Torres Beltran, M., Hallam, S.J. Microbial eukaryotic community structure and dynamics in a seasonally anoxic fjord, Saanich Inlet. ASLO/Ocean Sciences Meeting Honolulu, Hawaii 2/2014. Bernhard, J.M., Kormas, K., Pachiadaki, M.G., Rocke, E., Edgcomb, V.P. Halocline sediments ofdeep hypersaline anoxic basins support protist and fungal populations. ASLO/Ocean Sciences Meeting Honolulu, Hawaii 2/2014. Pachiadaki, M., Taylor, C., Yakimov, M., Bernhard, J., Edgcomb, V. Meta-Omics reveal new insight into microbial community activities along deep-sea redoxclines and adaptations to hypersalinity. ASLO/Ocean Sciences Meeting Honolulu, Hawaii 2/2014. Parris, D.J., Ganesh, S., DeLong, E.F., Edgcomb, V., Stewart, F.J. Microbial eukaryote diversity in the marine oxygen minimum zone off northern Chile. ASM Annual Meeting May 2014, Boston. Goldstein, S.T., Edgcomb, V.P., Bernhard, J.M. Warming oceans and potential range expansion of “smaller” benthic foraminifera: Insights from propagule experiments. GSA Annual Meeting, Denver: 125th Anniversary, Oct. 2013. Edgcomb, V.P. Protistan denizens of marine oxyclines and haloclines: submerged smorgasbords. New England Science Public 7th Biology New England South 2013 meeting, Bristol, RI Keynote Address 12/2013. Edgcomb, V.P. Symposium speaker: Ciliates and the Rare Biosphere Session: Protistan denizens of marine oxyclines and haloclines: submerged smorgasbords, ICOP, Vancouver, Canada, 2013. Salman, V., Edgcomb, V., Angert, E., Klein, F., Teske, A. Abstract: Novel large sulfur-oxidizing bacteria in Sippewissett Salt Marsh. ASM 5/2013. Summons, R.E., Gillespie, A.L., Schubotz, F., Welander, P.V., Pruss, S.B., Edgcomb, V.P., Bernhard, J.M., Beaudoin, D., Roberts, M., Beaupre, S., Sessions, A.L. Abstract: The biogeochemistry of ooids and oolites. IMOG Meeting of the European Association of Organic Geochemists. 9/2013.

V. EDGCOMB – 12 Edgcomb, V., Bernhard, J.M., Visscher, P.T., and Summons, R.E. Abstract: Eukaryotic communities of different microbialites in hypersaline Hamelin Pool, Shark Bay, W. Australia. 2013 ASLO Meeting, New Orleans, LA 2/2013. Bernhard, J.M., Edgcomb, V.P., Morrison, C., Orsi, W., Beaudoin, D.J. Abstract: Halocline sediments of deep hypersaline anoxic basins appear to support protist populations. Submitted for 2013 ASLO Ocean Sciences Meeting, New Orleans, LA 2/2013. Orsi, W., Biddle, J., Christman, G., Edgcomb, V. Abstract: Active fungi in the marine subsurface amidst a eukaryotic rRNA paleome. AGU 12/2012. Orsi, W., Edgcomb, V.P., Christmann, G., Biddle, J. Abstract: Gene expression in the deep subsurface. C-DEBI All Hands Meeting, Ventura Beach, CA. 10/2012. Orsi, W., Biddle, J., Edgcomb, V.P. Abstract: Active microbial eukaryotes amidst a marine subsurface paleome. 2012 International Society for Microbial Ecology (ISME) meeting in Copenhagen, Denmark 8/2012. Orsi, W., Song, Y., Hawley, A., Torres-Beltran, M., Hallam, S., Edgcomb, V. Abstract: Response of microbial eukaryotes to oxygen minimum zone formation. 2012 International Society for Microbial Ecology (ISME) meeting in Copenhagen, Denmark 8/2012. Pachiadaki, M., Oikonomou, A., Orsi, W., Edgcomb, V., Taylor, C., Yakimov, M., and Stoeck, T. Abstract: Assessing protistan grazing on marine prokaryotes in the mesopelagic realm of the Mediterranean Sea. 2012 International Society for Microbial Ecology (ISME) meeting in Copenhagen, Denmark 8/2012. Orsi, W., Biddle, J., Edgcomb, V. Abstract: Active microbial eukaryotes admidst a marine subsurface RNA paleome. Bioinformatics Workshop, Bremen, Germany, 6/2012. Bernhard, J.M., Edgcomb, V.P., Summons, R.E., Visscher, P.T., McIntyre-Wressnig, A. Abstract: Were foraminfera the beginning of the end for stromatolites? Geological Society of America (GSA) Annual Meeting, Charlotte, NC, 2012. Bernhard, J.M. and Edgcomb, V.P. Abstract: Protist strategies for chemocline living. International Society for Microbial Ecology 2012 Meeting Copenhagen, Denmark. Bernhard, J.M., McIntyre-Wressnig, A., Edgcomb, V.P. Abstract: Were foraminifera the beginning of the end for stromatolites? Field Workshop on Living Foraminifera in Japan, Sesoo Island (Okinawa) and Yokosuka (JAMSTEC;Kanagawa), 15-21 July 2012. Woehlke, S.A., Burgaud, G., Edgcomb, V., Biddle, J. Abstract: Defining marine fungi: cultivation tests of fungi along a salinity gradient of the Delaware Bay. 2012 Ocean Sciences Meeting, Salt Lake City, UT, February 2012. Edgcomb, V.P. Hutner Prize Address: The continued importance of field work: recent studies of protists in low-oxygen/anoxic marine environments, International Society of Protistologists, OSLO, Norway, 2012. Edgcomb, V.P., Bernhard, J.M., Visscher, P.T., McIntyre-Wressnig, A., Summons, R.E., and Beaudoin, D. Abstract: Diversity of microbial eukaryotes in stromatolites and thrombolites of Highborne Cay, Bahamas. Astrobiology Science Conference Atlanta, GA, 2012. Edgcomb, V.P. Protists not pickled by hypersalinity: microbial eukaryotes in deep hypersaline anoxic basins (E. Mediterranean Sea), International Society for Microbial Ecology (ISME) — Session on: Microbial Ecology in Extreme Environments - Copenhagen, Denmark (8/2012)

V. EDGCOMB – 13