Virginia P. Edgcomb

Total Page:16

File Type:pdf, Size:1020Kb

Virginia P. Edgcomb CURRICULUM VITAE VIRGINIA P. EDGCOMB Woods Hole Oceanographic Institution Current Position: Associate Scientist Department of Geology and Geophysics Tel: (508) 289-3734 220 McLean Lab, MS#8 Fax: (508) 457-2183 Woods Hole, MA 02543 e-mail: [email protected] GRADUATE EDUCATION 1997 Ph.D. Biology, University of Delaware (J. H. McDonald and D. W. Smith). Thesis title: Molecular Analysis of Spatial Heterogeneity of Sulfate-Reducing Bacteria in a Delaware Salt Marsh. RESEARCH EXPERIENCE/APPOINTMENTS 2014-Present Associate Scientist with Tenure, Woods Hole Oceanographic Institution. 2009–2014 Research Specialist, Woods Hole Oceanographic Institution. 2005–2009 Research Associate, Woods Hole Oceanographic Institution. 2002–2004 Visiting Investigator, Woods Hole Oceanographic Institution. 2000–2002 National Research Council Postdoctoral Associate at the Woods Hole Oceanographic Institution, Woods Hole, MA. Project focused on an assessment of the potential habitat range of selected hydrothermal vent hyperthermophillic archaea-based laboratory tests of their tolerance to various stressors typical of subsurface conditions. This project was funded by a NASA Astrobiology Institute/NRC postdoctoral associateship awarded to V.E. in 2000 (advisor: A. Teske, WHOI/UNC Chapel Hill). 1999–2000 Staff Scientist—The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, MA (with M.L. Sogin). 1998 University of Sydney, Australia. Visiting scholar, June-Sept. Protist isolation and cultivation from environmental samples, and electron microscopy (with D. Patterson). 1997–1999 Postdoctoral Fellow in The Josephine Bay Paul Center for Comparative Molecular Biology and Evolution. Projects included; isolation of novel protists from extreme environments, expanding phylogenies of deep-branching eukaryotes based on small subunit rRNA and protein coding genes, examining diversity of prokaryotic and eukaryotic microorganisms in extreme environments, particularly hydrothermal vent and other anaerobic freshwater and marine sediments using molecular and culture-based approaches (with M.L. Sogin). 1997 Marine Biological Laboratory, Woods Hole, MA Workshop on Molecular Evolution. 1997 United States Environmental Protection Agency Microbial Ecology Branch, Gulf Breeze, Florida. Visiting scholar working on molecular microbial ecology. AWARDS/FELLOWSHIPS 2012 Seymour H. Hutner Prize in Protistology (awarded by International Society of Protistologists) 2001 NASA/NRC Astrobiology Institute Postdoctoral Associateship Award (admin. by the NRC renewal). 2000 NASA/NRC Astrobiology Institute Postdoctoral Associateship Award (admin. by the NRC). V. EDGCOMB – 1 1999 Award from Gordon Research Conferences to attend the 1999 Gordon Conference on Molecular Evolution in Hayama, Japan. 1997 Block Fellowship. A competitive fellowship awarded to a Biology Department graduate student in recognition of outstanding achievement, University of Delaware. TEACHING EXPERIENCE 2002-present Part-time Life Science Teacher, Falmouth Academy, Falmouth, MA. 2003–2008 Lecturer and laboratory instructor for “Living in the Microbial World” secondary science teacher training workshops (NASA) Marine Biological Laboratory, Woods Hole, MA. 1998 Course Assistant, Workshop on Molecular Evolution, Marine Biological Laboratory, Woods Hole, MA. 1997 Course Instructor, University of Delaware-Microbiology. 1992–1997 Graduate teaching assistant, Department of Biology, University of Delaware. PROFESSIONAL AFFILIATIONS • American Society of Microbiologists • International Society of Protistologists • American Society for Limnology and Oceanography • International Society for Microbial Ecology PROFESSIONAL ACTIVITIES • President-elect 2014 International Society of Protistologists • Full Member: Science Scientific Committee on Oceanic Research (SCOR) Working Group on Microbial Community Responses to Ocean Deoxygenation (2014-2019) • EarthCube End User Domain Workshop for Deep-Sea Processes and Dynamics, URI Graduate School of Oceanography, Narragansett, RI, June, 2013. • Moore Foundation Workshop on Microbial Ecology and Biogeochemistry of Oxygen-Deficient Marine Waters (OMZ) in Santa Cruz, Chile, 3/2013. • Associate Editor, Frontiers in Extreme Microbiology (2010-present) • Review Editor, Frontiers in Microbial Symbioses (2014-present) • Review Editor, Frontiers in Marine Molecular Biology and Ecology (2014-present) • Special Topics Editor, Deep Subsurface Microbiology, Frontiers in Extreme Microbiology (2012) • Editorial Advisor of New England Science Public Series on Evolution. • Special Topics Editor, Eukaryotes in Extreme Environments, Frontiers in Microbiology (2012) • Co-Chair of Special Session at Aquatic Sciences Meeting, New Orleans - Microbial Ecology of Hypersaline Habitats 2012 • Assoc. member SCOR Working Group 134 — Microbial Carbon Pump in the Ocean (2012) • Development of a WEB-based resource for COSEE NOW (Centers for Ocean Sciences Education Excellence, Networked Ocean World) to help scientists to prepare more effective Broader Impact Statements for NSF Criterion II, January, 2012 • NSF Panel on Shipboard Technical Services, February, 2012 V. EDGCOMB – 2 • Annual 2-day summer workshop on Marine Biotechnology for high school students, through the Weston High School Advanced Biotechnology Institute (2010 to present) • Initiated trimester internship program between Falmouth Academy high school students and WHOI science laboratories — first program year 2011-2012 • Participant in IODP “Building U.S. Strategies for 2013-2023 Scientific Ocean Drilling” workshop, Denver, CO 5/2012 • Dive and Discover Expeditions to the Seafloor — Expedition 14 (2011) • Chair, Nominating Committee, ISOP 2010-11 • Chair of Special Session at ASLO 2011, San Juan Puerto Rico — Protists in Extreme Environments: Beyond Diversity. • Session Leader C-DEBI RCN, Microbial activity, physiology, metabolism 2011 • Board of Directors, Zephyr Education Foundation 2010 to present • Education Advisory Committee for Waquoit Bay National Estuarine Research Reserve (2011 to present) • 2012-2015 WHOI Partnership Program • NSF Panel on Oceanographic Instrumentation/Shipboard Scientific Support Equipment, January 2010. • WHOI Women’s Committee (2010 to 2013) OTHER PROFESSIONAL ACTIVITIES: MANUSCRIPT/PROPOSAL REVIEWER Nature Geosciences National Science Foundation Journal of Eukaryotic Microbiology Limnology and Oceanography Molecular Biology and Evolution NASA Ecology Extremophiles Aquatic Microbial Ecology Environmental Microbiology Applied and Environmental Microbiology Protist Trends in Ecology and Evolution ISME Journal PNAS Microbial Ecology Frontiers FEMS Microbiology Letters Molecular Ecology Resources FEMS Microbial Ecology NEW TECHNOLOGY DEVELOPMENT Development of new technology for in situ studies in marine microbial ecology, the Microbial Sampler - Submersible Incubation Device, MS-SID) together with Craig Taylor (WHOI Biology) and McLane Research Laboratories, E. Falmouth, MA. Retrofitting and application of an older technology (Deep-SID) to in situ fixation of water samples for microscopy in Cariaco Basin with Craig Taylor (WHOI Biology). GROUP MEMBERS AND GUESTS CURRENT • Postdoc, Maria Pachiadaki, Oct. 2012 to present. V. EDGCOMB – 3 • Postdoc, Roxanne Beinart, Dec. 2013 to present. • Research Associate, David Beaudoin, assists on various research projects, 2006–present. • Dr. Sofia Ibarraran • Thesis committee member for PhD candidates Joseph Russell (U. Delaware, 2011-present), Elizabeth Suter (Stony Brook University 2013-present) GROUP MEMBERS AND GUESTS PAST • Dr. Konstantinos Kormas, sabbatical visitor 2013 • Postdoc, William T. Orsi, 2011– 2013. • PhD candidate Vanessa Redou, Laboratoire de Biodiversite et Ecologie Microbienne, ESMISAB, France, 2/2014-5/2014) and PhD committee member. • Masters candidate Manon Duret, Universite du Littoral, CNRS-UMR, France (1/2014-6/2014) • PhD candidate Emma Louise Rocke, Hong Kong University of Science and Technology (6/2013- 12/2013-6/2013) and PhD committee member. • Joseph Russell, U. Delaware, in my laboratory 3/2012, Ph.D. committee member 2011-present. • Sophie Charvet, U. Laval, Canada, as Guest Graduate Student in my laboratory spring 2012. • Verena Salman, Max-Planck, Germany as Guest Investigator in my laboratory 8/1/2012–9/30/2012. • Gaëtan Burgaud, U. Brest, France as Guest Investigator 1/2011–5/2011. • Dr. Ankita Srivastava, 2013-14. • Summer Undergraduate Guest Students: Vincent Klokman from Univ. North Carolina, summer 2009; Anja Hickethier, Kaiserslautern University, Germany, spring 2010; Greg Gotta, St. Lawrence University, summer 2010; Brecia Douglas, Northeastern U., summer 2011; Colin Morrison, U. Nevada Reno (co-advised with J. Bernhard), summer 2012. • MS candidate Aimee Gillespie, MIT. Committee member and supervisor of her molecular microbial ecology work, 2011–2012. • PhD candidate William Orsi, Northeastern University. PhD committee member and co-supervisor with Slava Epstein (Northeastern U., his primary thesis advisor) of the environmental sequencing portion of his thesis project (2007-2011), and supervisor of his work on the E. Mediterranean “Pickled Protists” project, 2007–2011. • Undergraduate Research Fellows Simone Boer and Karen Lloyd, while working in Teske Lab on pressure, sulfide, heavy metal, and pH tolerance of deep-sea hyperthermophiles 2000-2002. • Technician, David Kysela, working on molecular survey of prokaryotic and eukaryotic diversity in Guaymas Basin hydrothermal sediments,
Recommended publications
  • A Wide Diversity of Previously Undetected Freeliving
    Environmental Microbiology (2010) 12(10), 2700–2710 doi:10.1111/j.1462-2920.2010.02239.x A wide diversity of previously undetected free-living relatives of diplomonads isolated from marine/saline habitatsemi_2239 2700..2710 Martin Kolisko,1 Jeffrey D. Silberman,2 Kipferlia n. gen. The remaining isolates include rep- Ivan Cepicka,3 Naoji Yubuki,4† Kiyotaka Takishita,5 resentatives of three other lineages that likely repre- Akinori Yabuki,4 Brian S. Leander,6 Isao Inouye,4 sent additional undescribed genera (at least). Small- Yuji Inagaki,7 Andrew J. Roger8 and subunit ribosomal RNA gene phylogenies show that Alastair G. B. Simpson1* CLOs form a cloud of six major clades basal to the Departments of 1Biology and 8Biochemistry and diplomonad-retortamonad grouping (i.e. each of the Molecular Biology, Dalhousie University, Halifax, Nova six CLO clades is potentially as phylogenetically Scotia, Canada. distinct as diplomonads and retortamonads). CLOs 2Department of Biological Sciences, University of will be valuable for tracing the evolution of Arkansas, Fayetteville, AR, USA. diplomonad cellular features, for example, their 3Department of Zoology, Faculty of Science, Charles extremely reduced mitochondrial organelles. It is University in Prague, Prague, Czech Republic. striking that the majority of CLO diversity was unde- 4Institute of Biological Sciences, Graduate School of Life tected by previous light microscopy surveys and and Environmental Sciences and 7Center for environmental PCR studies, even though they inhabit Computational Sciences and Institute of Biological a commonly sampled environment. There is no Sciences, University of Tsukuba, Tsukuba, Ibaraki, reason to assume this is a unique situation – it is Japan. likely that undersampling at the level of major lin- 5Japan Agency for Marine-Earth Science and eages is still widespread for protists.
    [Show full text]
  • Molecular Identification and Evolution of Protozoa Belonging to the Parabasalia Group and the Genus Blastocystis
    UNIVERSITAR DEGLI STUDI DI SASSARI SCUOLA DI DOTTORATO IN SCIENZE BIOMOLECOLARI E BIOTECNOLOGICHE (Intenational PhD School in Biomolecular and Biotechnological Sciences) Indirizzo: Microbiologia molecolare e clinica Molecular identification and evolution of protozoa belonging to the Parabasalia group and the genus Blastocystis Direttore della scuola: Prof. Masala Bruno Relatore: Prof. Pier Luigi Fiori Correlatore: Dott. Eric Viscogliosi Tesi di Dottorato : Dionigia Meloni XXIV CICLO Nome e cognome: Dionigia Meloni Titolo della tesi : Molecular identification and evolution of protozoa belonging to the Parabasalia group and the genus Blastocystis Tesi di dottorato in scienze Biomolecolari e biotecnologiche. Indirizzo: Microbiologia molecolare e clinica Universit degli studi di Sassari UNIVERSITAR DEGLI STUDI DI SASSARI SCUOLA DI DOTTORATO IN SCIENZE BIOMOLECOLARI E BIOTECNOLOGICHE (Intenational PhD School in Biomolecular and Biotechnological Sciences) Indirizzo: Microbiologia molecolare e clinica Molecular identification and evolution of protozoa belonging to the Parabasalia group and the genus Blastocystis Direttore della scuola: Prof. Masala Bruno Relatore: Prof. Pier Luigi Fiori Correlatore: Dott. Eric Viscogliosi Tesi di Dottorato : Dionigia Meloni XXIV CICLO Nome e cognome: Dionigia Meloni Titolo della tesi : Molecular identification and evolution of protozoa belonging to the Parabasalia group and the genus Blastocystis Tesi di dottorato in scienze Biomolecolari e biotecnologiche. Indirizzo: Microbiologia molecolare e clinica Universit degli studi di Sassari Abstract My thesis was conducted on the study of two groups of protozoa: the Parabasalia and Blastocystis . The first part of my work was focused on the identification, pathogenicity, and phylogeny of parabasalids. We showed that Pentatrichomonas hominis is a possible zoonotic species with a significant potential of transmission by the waterborne route and could be the aetiological agent of gastrointestinal troubles in children.
    [Show full text]
  • Author's Manuscript (764.7Kb)
    1 BROADLY SAMPLED TREE OF EUKARYOTIC LIFE Broadly Sampled Multigene Analyses Yield a Well-resolved Eukaryotic Tree of Life Laura Wegener Parfrey1†, Jessica Grant2†, Yonas I. Tekle2,6, Erica Lasek-Nesselquist3,4, Hilary G. Morrison3, Mitchell L. Sogin3, David J. Patterson5, Laura A. Katz1,2,* 1Program in Organismic and Evolutionary Biology, University of Massachusetts, 611 North Pleasant Street, Amherst, Massachusetts 01003, USA 2Department of Biological Sciences, Smith College, 44 College Lane, Northampton, Massachusetts 01063, USA 3Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, 7 MBL Street, Woods Hole, Massachusetts 02543, USA 4Department of Ecology and Evolutionary Biology, Brown University, 80 Waterman Street, Providence, Rhode Island 02912, USA 5Biodiversity Informatics Group, Marine Biological Laboratory, 7 MBL Street, Woods Hole, Massachusetts 02543, USA 6Current address: Department of Epidemiology and Public Health, Yale University School of Medicine, New Haven, Connecticut 06520, USA †These authors contributed equally *Corresponding author: L.A.K - [email protected] Phone: 413-585-3825, Fax: 413-585-3786 Keywords: Microbial eukaryotes, supergroups, taxon sampling, Rhizaria, systematic error, Excavata 2 An accurate reconstruction of the eukaryotic tree of life is essential to identify the innovations underlying the diversity of microbial and macroscopic (e.g. plants and animals) eukaryotes. Previous work has divided eukaryotic diversity into a small number of high-level ‘supergroups’, many of which receive strong support in phylogenomic analyses. However, the abundance of data in phylogenomic analyses can lead to highly supported but incorrect relationships due to systematic phylogenetic error. Further, the paucity of major eukaryotic lineages (19 or fewer) included in these genomic studies may exaggerate systematic error and reduces power to evaluate hypotheses.
    [Show full text]
  • Supporting Material
    Supporting Text Recursions. We developed a model to investigate the evolution of ploidy levels in the presence of host-parasite interactions between a focal and nonfocal species. The focal species is assumed to have two loci, a ploidy modifier locus with alleles C1 and C2 and an interaction locus with alleles A and a. Thus there are four haploid gamete types in the focal species: AC1 with frequency X1, aC1 with frequency X2, AC2 with frequency X3, and aC2 with frequency X4. The modifier locus influences ploidy levels by altering the timing of meiosis; diploid zygotes of genotype CiCj have a probability, dij, of undergoing meiosis late in life, thus experiencing host-parasite selection as a diploid, versus early in life, thus experiencing selection as a haploid (Fig. 2). The nonfocal species is assumed to be a sexual diploid, having only a brief haploid stage, although results derived with a haploid nonfocal species were similar. For clarity, we assume that the A locus in the focal species interacts with a B locus in the nonfocal species, with alleles B and b. Note that Table 1 differs from this convention by referring to alleles in both species as A and a. We use a different notation here to avoid additional subscripts in the equations. The frequencies of alleles are denoted by pA, pa, pC1, and pC2 in the focal species and pB and pb in the nonfocal species, all measured at the gamete stage of the life cycle (Fig. 2). Also measured at this stage is D = (X1 X4 - X2 X3), the disequilibrium between the modifier and selected loci in the focal species.
    [Show full text]
  • A Free-Living Protist That Lacks Canonical Eukaryotic DNA Replication and Segregation Systems
    bioRxiv preprint doi: https://doi.org/10.1101/2021.03.14.435266; this version posted March 15, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 A free-living protist that lacks canonical eukaryotic DNA replication and segregation systems 2 Dayana E. Salas-Leiva1, Eelco C. Tromer2,3, Bruce A. Curtis1, Jon Jerlström-Hultqvist1, Martin 3 Kolisko4, Zhenzhen Yi5, Joan S. Salas-Leiva6, Lucie Gallot-Lavallée1, Geert J. P. L. Kops3, John M. 4 Archibald1, Alastair G. B. Simpson7 and Andrew J. Roger1* 5 1Centre for Comparative Genomics and Evolutionary Bioinformatics (CGEB), Department of 6 Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS, Canada, B3H 4R2 2 7 Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom 8 3Oncode Institute, Hubrecht Institute – KNAW (Royal Netherlands Academy of Arts and Sciences) 9 and University Medical Centre Utrecht, Utrecht, The Netherlands 10 4Institute of Parasitology Biology Centre, Czech Acad. Sci, České Budějovice, Czech Republic 11 5Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, School of Life Science, 12 South China Normal University, Guangzhou 510631, China 13 6CONACyT-Centro de Investigación en Materiales Avanzados, Departamento de medio ambiente y 14 energía, Miguel de Cervantes 120, Complejo Industrial Chihuahua, 31136 Chihuahua, Chih., México 15 7Centre for Comparative Genomics and Evolutionary Bioinformatics (CGEB), Department of 16 Biology, Dalhousie University, Halifax, NS, Canada, B3H 4R2 17 *corresponding author: [email protected] 18 D.E.S-L ORCID iD: 0000-0003-2356-3351 19 E.C.T.
    [Show full text]
  • Phylogenomic Analyses Support the Monophyly of Excavata and Resolve Relationships Among Eukaryotic ‘‘Supergroups’’
    Phylogenomic analyses support the monophyly of Excavata and resolve relationships among eukaryotic ‘‘supergroups’’ Vladimir Hampla,b,c, Laura Huga, Jessica W. Leigha, Joel B. Dacksd,e, B. Franz Langf, Alastair G. B. Simpsonb, and Andrew J. Rogera,1 aDepartment of Biochemistry and Molecular Biology, Dalhousie University, Halifax, NS, Canada B3H 1X5; bDepartment of Biology, Dalhousie University, Halifax, NS, Canada B3H 4J1; cDepartment of Parasitology, Faculty of Science, Charles University, 128 44 Prague, Czech Republic; dDepartment of Pathology, University of Cambridge, Cambridge CB2 1QP, United Kingdom; eDepartment of Cell Biology, University of Alberta, Edmonton, AB, Canada T6G 2H7; and fDepartement de Biochimie, Universite´de Montre´al, Montre´al, QC, Canada H3T 1J4 Edited by Jeffrey D. Palmer, Indiana University, Bloomington, IN, and approved January 22, 2009 (received for review August 12, 2008) Nearly all of eukaryotic diversity has been classified into 6 strong support for an incorrect phylogeny (16, 19, 24). Some recent suprakingdom-level groups (supergroups) based on molecular and analyses employ objective data filtering approaches that isolate and morphological/cell-biological evidence; these are Opisthokonta, remove the sites or taxa that contribute most to these systematic Amoebozoa, Archaeplastida, Rhizaria, Chromalveolata, and Exca- errors (19, 24). vata. However, molecular phylogeny has not provided clear evi- The prevailing model of eukaryotic phylogeny posits 6 major dence that either Chromalveolata or Excavata is monophyletic, nor supergroups (25–28): Opisthokonta, Amoebozoa, Archaeplastida, has it resolved the relationships among the supergroups. To Rhizaria, Chromalveolata, and Excavata. With some caveats, solid establish the affinities of Excavata, which contains parasites of molecular phylogenetic evidence supports the monophyly of each of global importance and organisms regarded previously as primitive Rhizaria, Archaeplastida, Opisthokonta, and Amoebozoa (16, 18, eukaryotes, we conducted a phylogenomic analysis of a dataset of 29–34).
    [Show full text]
  • The Phylogenetic Position of Enteromonads: a Challenge for the Present Models of Diplomonad Evolution
    International Journal of Systematic and Evolutionary Microbiology (2005), 55, 1729–1733 DOI 10.1099/ijs.0.63542-0 The phylogenetic position of enteromonads: a challenge for the present models of diplomonad evolution Martin Kolisko, Ivan Cepicka, Vladimı´r Hampl, Jaroslav Kulda and Jaroslav Flegr Correspondence Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic Martin Kolisko [email protected] Unikaryotic enteromonads and diplokaryotic diplomonads have been regarded as closely related protozoan groups. It has been proposed that diplomonads originated within enteromonads in a single event of karyomastigont duplication. This paper presents the first study to address these questions using molecular phylogenetics. The sequences of the small-subunit rRNA genes for three isolates of enteromonads were determined and a tree constructed with available diplomonad, retortamonad and Carpediemonas sequences. The diplomonad sequences formed two main groups, with the genus Giardia on one side and the genera Spironucleus, Hexamita and Trepomonas on the other. The three enteromonad sequences formed a clade robustly situated within the diplomonads, a position inconsistent with the original evolutionary proposal. The topology of the tree indicates either that the diplokaryotic cell of diplomonads arose several times independently, or that the monokaryotic cell of enteromonads originated by secondary reduction from the diplokaryotic state. INTRODUCTION retortamonads constituted a sister clade of the diplomonad
    [Show full text]
  • Systema Naturae. the Classification of Living Organisms
    Systema Naturae. The classification of living organisms. c Alexey B. Shipunov v. 5.601 (June 26, 2007) Preface Most of researches agree that kingdom-level classification of living things needs the special rules and principles. Two approaches are possible: (a) tree- based, Hennigian approach will look for main dichotomies inside so-called “Tree of Life”; and (b) space-based, Linnaean approach will look for the key differences inside “Natural System” multidimensional “cloud”. Despite of clear advantages of tree-like approach (easy to develop rules and algorithms; trees are self-explaining), in many cases the space-based approach is still prefer- able, because it let us to summarize any kinds of taxonomically related da- ta and to compare different classifications quite easily. This approach also lead us to four-kingdom classification, but with different groups: Monera, Protista, Vegetabilia and Animalia, which represent different steps of in- creased complexity of living things, from simple prokaryotic cell to compound Nature Precedings : doi:10.1038/npre.2007.241.2 Posted 16 Aug 2007 eukaryotic cell and further to tissue/organ cell systems. The classification Only recent taxa. Viruses are not included. Abbreviations: incertae sedis (i.s.); pro parte (p.p.); sensu lato (s.l.); sedis mutabilis (sed.m.); sedis possi- bilis (sed.poss.); sensu stricto (s.str.); status mutabilis (stat.m.); quotes for “environmental” groups; asterisk for paraphyletic* taxa. 1 Regnum Monera Superphylum Archebacteria Phylum 1. Archebacteria Classis 1(1). Euryarcheota 1 2(2). Nanoarchaeota 3(3). Crenarchaeota 2 Superphylum Bacteria 3 Phylum 2. Firmicutes 4 Classis 1(4). Thermotogae sed.m. 2(5).
    [Show full text]
  • Marine Biological Laboratory) Data Are All from EST Analyses
    TABLE S1. Data characterized for this study. rDNA 3 - - Culture 3 - etK sp70cyt rc5 f1a f2 ps22a ps23a Lineage Taxon accession # Lab sec61 SSU 14 40S Actin Atub Btub E E G H Hsp90 M R R T SUM Cercomonadida Heteromita globosa 50780 Katz 1 1 Cercomonadida Bodomorpha minima 50339 Katz 1 1 Euglyphida Capsellina sp. 50039 Katz 1 1 1 1 4 Gymnophrea Gymnophrys sp. 50923 Katz 1 1 2 Cercomonadida Massisteria marina 50266 Katz 1 1 1 1 4 Foraminifera Ammonia sp. T7 Katz 1 1 2 Foraminifera Ovammina opaca Katz 1 1 1 1 4 Gromia Gromia sp. Antarctica Katz 1 1 Proleptomonas Proleptomonas faecicola 50735 Katz 1 1 1 1 4 Theratromyxa Theratromyxa weberi 50200 Katz 1 1 Ministeria Ministeria vibrans 50519 Katz 1 1 Fornicata Trepomonas agilis 50286 Katz 1 1 Soginia “Soginia anisocystis” 50646 Katz 1 1 1 1 1 5 Stephanopogon Stephanopogon apogon 50096 Katz 1 1 Carolina Tubulinea Arcella hemisphaerica 13-1310 Katz 1 1 2 Cercomonadida Heteromita sp. PRA-74 MBL 1 1 1 1 1 1 1 7 Rhizaria Corallomyxa tenera 50975 MBL 1 1 1 3 Euglenozoa Diplonema papillatum 50162 MBL 1 1 1 1 1 1 1 1 8 Euglenozoa Bodo saltans CCAP1907 MBL 1 1 1 1 1 5 Alveolates Chilodonella uncinata 50194 MBL 1 1 1 1 4 Amoebozoa Arachnula sp. 50593 MBL 1 1 2 Katz lab work based on genomic PCRs and MBL (Marine Biological Laboratory) data are all from EST analyses. Culture accession number is ATTC unless noted. GenBank accession numbers for new sequences (including paralogs) are GQ377645-GQ377715 and HM244866-HM244878.
    [Show full text]
  • Multi-Gene Phylogenetic Analysis of the Supergroup Excavata
    MULTI-GENE PHYLOGENETIC ANALYSIS OF THE SUPERGROUP EXCAVATA By CHRISTINA CASTLEJOHN (Under the Direction of Mark A. Farmer) ABSTRACT The supergroup Excavata, one of six supergroups of eukaryotes, has been a controversial supergroup within the Eukaryotic Tree of Life. Excavata was originally based largely on morphological data and to date has not been well supported by molecular studies. The goals of this research were to test the monophyly of Excavata and to observe relationships among the nine subgroups of excavates included in this study. Several different types of phylogenetic analyses were performed on a data set consisting of sequences from nine reasonably conserved genes. Analyses of this data set recovered monophyly of Excavata with moderate to strong support. Topology tests rejected all but two topologies: one with a monophyletic Excavata and one with Excavata split into two major clades. Simple gap coding, which was performed on the ribosomal DNA alignments, was found to be more useful for species-level analyses than deeper relationships with the eukaryotes. INDEX WORDS: Excavata, excavates, monophyly, phylogenetic analysis, gap coding MULTI-GENE PHYLOGENETIC ANALYSIS OF THE SUPERGROUP EXCAVATA By CHRISTINA CASTLEJOHN B.S., Georgia Institute of Technology, 2002 A Thesis Submitted to the Graduate Faculty of The University of Georgia in Partial Fulfillment of the Requirements for the Degree MASTER OF SCIENCE ATHENS, GEORGIA 2009 © 2009 Christina Castlejohn All Rights Reserved MULTI-GENE PHYLOGENETIC ANALYSIS OF THE SUPERGROUP EXCAVATA By CHRISTINA CASTLEJOHN Major Professor: Mark A. Farmer Committee: James Leebens-Mack Joseph McHugh Electronic Version Approved: Maureen Grasso Dean of the Graduate School The University of Georgia August 2009 iv DEDICATION To my family, who have supported me in my journey v ACKNOWLEDGEMENTS I would like to thank Mark Farmer for helping me so much in my pursuit of higher education and my plans for the future.
    [Show full text]
  • Giardia Intestinalis and Complex Evolutionary Dynamics Spanning the Transition to Parasitism in the Lineage Fornicata Shweta V
    Pipaliya et al. BMC Biology (2021) 19:167 https://doi.org/10.1186/s12915-021-01077-2 RESEARCH ARTICLE Open Access Unexpected organellar locations of ESCRT machinery in Giardia intestinalis and complex evolutionary dynamics spanning the transition to parasitism in the lineage Fornicata Shweta V. Pipaliya1† , Rui Santos2† , Dayana Salas-Leiva3 , Erina A. Balmer4 , Corina D. Wirdnam4 , Andrew J. Roger3 , Adrian B. Hehl2 , Carmen Faso4,6* and Joel B. Dacks1,5,7* Abstract Background: Comparing a parasitic lineage to its free-living relatives is a powerful way to understand how that evolutionary transition to parasitism occurred. Giardia intestinalis (Fornicata) is a leading cause of gastrointestinal disease world-wide and is famous for its unusual complement of cellular compartments, such as having peripheral vacuoles instead of typical endosomal compartments. Endocytosis plays an important role in Giardia’s pathogenesis. Endosomal sorting complexes required for transport (ESCRT) are membrane-deforming proteins associated with the late endosome/multivesicular body (MVB). MVBs are ill-defined in G. intestinalis, and roles for identified ESCRT- related proteins are not fully understood in the context of its unique endocytic system. Furthermore, components thought to be required for full ESCRT functionality have not yet been documented in this species. Results: We used genomic and transcriptomic data from several Fornicata species to clarify the evolutionary genome streamlining observed in Giardia, as well as to detect any divergent orthologs of the Fornicata ESCRT subunits. We observed differences in the ESCRT machinery complement between Giardia strains. Microscopy-based investigations of key components of ESCRT machinery such as GiVPS36 and GiVPS25 link them to peripheral vacuoles, highlighting these organelles as simplified MVB equivalents.
    [Show full text]
  • BMC Evolutionary Biology Biomed Central
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Springer - Publisher Connector BMC Evolutionary Biology BioMed Central Research article Open Access An ancient spliceosomal intron in the ribosomal protein L7a gene (Rpl7a) of Giardia lamblia Anthony G Russell†, Timothy E Shutt†, Russell F Watkins and Michael W Gray* Address: Program in Evolutionary Biology, Canadian Institute for Advanced Research, Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia B3H 1X5, Canada Email: Anthony G Russell - [email protected]; Timothy E Shutt - [email protected]; Russell F Watkins - [email protected]; Michael W Gray* - [email protected] * Corresponding author †Equal contributors Published: 18 August 2005 Received: 22 April 2005 Accepted: 18 August 2005 BMC Evolutionary Biology 2005, 5:45 doi:10.1186/1471-2148-5-45 This article is available from: http://www.biomedcentral.com/1471-2148/5/45 © 2005 Russell et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Only one spliceosomal-type intron has previously been identified in the unicellular eukaryotic parasite, Giardia lamblia (a diplomonad). This intron is only 35 nucleotides in length and is unusual in possessing a non-canonical 5' intron boundary sequence, CT, instead of GT. Results: We have identified a second spliceosomal-type intron in G. lamblia, in the ribosomal protein L7a gene (Rpl7a), that possesses a canonical GT 5' intron boundary sequence.
    [Show full text]