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TRAF2 Sirna Set I TRAF2 Sirna Set I

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TRAF2 Sirna Set I TRAF2 Sirna Set I Catalog # Aliquot Size T57-911-05 3 x 5 nmol T57-911-20 3 x 20 nmol T57-911-50 3 x 50 nmol TRAF2 siRNA Set I siRNA duplexes targeted against three exon regions Catalog # T57-911 Lot # Z2097-85 Specificity Formulation TRAF2 siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human TRAF2 expression. shipped at room temperature. Product Description Reconstitution Protocol TRAF2 siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human TRAF2 mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The gene Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_021138. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot TRAP, TRAP3, MGC: 45012 1x stock solutions for storage. Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or TRAF2 Protein T57-30H below -20oC. After resuspension, siRNA stock solutions ≥2 Anti-TRAF2 T57-363BR µM can undergo up to 50 freeze-thaw cycles without Anti-TRAF2 T57-363R significant degradation. For long-term storage, it is Anti-TRAF3 T58-363R recommended that the siRNA is stored at -70oC. For most Anti-TRAF3 T58-363BR favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Scientific Background TRAF2 (also known as TNF receptor-associated factor 2) TRAF2 siRNA Set I belongs to the TNF receptor associated factor (TRAF) siRNA duplexes targeted against three exon regions protein family that mediates signal transduction from members of the TNF receptor superfamily (1). TRAF2 is Catalog # T57-911 involved in TNF-alpha-mediated activation of MAPK8/JNK Lot # Z2097-85 and NF-B pathway. TRAF2 interacts with TNF receptors Packaging Specifications 2.5 nmol/tube for 3 x 5nmol and forms a heterodimeric complex with TRAF1. The Format Lyophilized powder TRAF1-TRAF2 complex interacts with IAPs to mediate the Stability 1yr at -70oC from date of shipment anti-apoptotic signals from TNF receptors. TRAF2 also Storage & Shipping The lyophilized powder is stable for at interacts with TRADD, a TNF receptor associated least 4 weeks at room temperature. It is apoptotic signal transducer, to ensure the recruitment of recommended that the lyophilized and resuspended siRNAs are stored at IAPs for the direct inhibition of caspase activation (2). or below -20oC. After resuspension, siRNA stock solutions ≥2 µM can undergo up to 50 freeze-thaw cycles References without significant degradation. For 1. Wajant, H. Et al: The TNF-receptor-associated factor long-term storage, it is recommended family: scaffold molecules for cytokine receptors, that the siRNA is stored at -70oC. For most favorable performance, avoid kinases and their regulators.". Cell. Signal. 2001;13 (6): repeated handling and multiple 389–400. freeze/thaw cycles. 2. Bradley, J.R., Pober, J.S. Tumor necrosis factor receptor-associated factors (TRAFs).". Oncogene,2001; 20 (44): 6482–91 To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .
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