Data Sheets Biowave

Total Page:16

File Type:pdf, Size:1020Kb

Data Sheets Biowave Biowave DNA A DEDICATED LIFE SCIENCE BASED INSTRUMENT WITH STORED ROUTINES FOR NUCLEIC ACID, PROTEIN AND Life Science Spectrophotometer CELL DENSITY MEASUREMENTS. A WORKHORSE FOR THE MOLECULAR BIOLOGIST The Biowave DNA has been specifically designed for life ■ NOVEL GIFFORD OPTICS FOR HIGH science applications and is a powerful tool for the laboratory ENERGY COMBINED WITH A XENON that requires a dedicated instrument for the determination of nucleic acid purity and concentration, protein SOURCE FOR LONG LAMP LIFETIME concentrations or cell density measurements. ■ SIMPLE SELECTION SOFTWARE WITH The system utilises Gifford Optics for high energy STORED METHODS FOR LIFE SCIENCE throughput, a Xenon light source for long lamp lifetimes APPLICATIONS together with simple selection software and large graphical display for ease of use and data interpretation. The stored ■ FULL GRAPHICS DISPLAY methods include DNA, RNA and oligonucleotide calculations, protein assays such as direct UV measurement, ■ NUCLEIC ACID SCANS FOR PURITY BCA, Biuret, Bradford and Lowry and cell density CHECKING. measurement. Unlike many dedicated life science ■ INTEGRATED PRINTER (OPTION) instruments the Biowave DNA can also measure Absorbance or concentration at any wavelength so there is complete ■ COMPACT SPACE SAVING DESIGN flexibility for future applications. ■ COMPATIBLE WITH LOW VOLUME For added convenience it is possible to display a scan of the nucleic acid profile which is particularly useful for RNA CUVETTES samples where impurities may be present in the 230 nm ■ UNIQUE, INTEGRAL CUVETTE TRAY region, yet not have an adverse effect on the 260/280 Absorbance ratio. The system is compatible with both Quartz FOR SECURELY HOLDING EXPENSIVE and disposable low volume UV cuvettes. CELLS AND VALUABLE SAMPLES Results may be printed to an optional integrated high quality graphical printer for a permanent record or exported via a USB connection to a suitable PC running optional Print Via Computer (PVC) software for advanced reporting or data storage. Colorimeters and UV/Visible Spectrophotometers HOME PAGE DNA SCAN The Biowave DNA is optimised for the The standard nucleic acid methods life scientist who is involved in nucleic include the ability to display a acid quantification or protein wavelength scan of the region. This determinations. System set up could allows a visual check for imputities and this data may be printed or not be easier using the simple exported as additional proof of selection software together with the analysis. pre-stored methods for all aspects of DNA, RNA, Oligonucleotides, proteins and cell culture determinations. NUCLEIC ACIDS PROTEINS The instrument displays 260/280 and Pre-stored protein methods include 260/230 ratios plus concentration direct UV measurement, BCA, including compensation for Bradford, Lowry and Biuret with the pathlength and dilution factor. option of curve fitting routines on up Background correction is available at to 9 standards in triplicate. The 320nm if required for buffer calibration curve is displayed with compensation or when using reduced statistical data as part of the aperture cells. comprehensive data handling routines All the data is displayed on one screen and the curve can be stored for future for convenience. use. Technical Specifications Ordering Guide Biochrom Limited Stored methods 9 Biowave DNA UV/Visible Life Science Spectrophotometer 80-3004-70 22 Cambridge Science Park, Wavelength accuracy ± 2nm Biowave DNA UV/Visible Life Science Spectrophotometer with printer 80-3004-71 Cambridge, CB4 0FJ England Photometric reproducibility ± 0.002A at 0-0.5A, 546nm Printer accessory 80-3003-84 Tel: +44 (0)1223 423723 Photometric accuracy ± 0.003A at 0-0.5A Spare printer paper (20 rolls) 80-3004-07 Fax: +44 (0)1223 420164 Outputs USB Print via computer software and cable 80-3004-73 Email: [email protected] Dimensions (W x D x H) 260 x 390 x 100mm Web: www.biochrom.co.uk Weight <4.5 kg 80-3004-75 WPA belongs to the Biochrom group of companies..
Recommended publications
  • The Role of SAMHD1 in Restriction and Immune Sensing of Retroviruses and Retroelements
    The role of SAMHD1 in restriction and immune sensing of retroviruses and retroelements Die Rolle von SAMHD1 in der Restriktion und Immunerkennung von Retroviren und Retroelementen Der Naturwissenschaftlichen Fakultät der Friedrich-Alexander-Universität Erlangen-Nürnberg zur Erlangung des Doktorgrades Dr. rer. nat. vorgelegt von Alexandra Herrmann aus Biberach an der Riß Als Dissertation genehmigt von der Naturwissenschaftlichen Fakultät der Friedrich-Alexander-Universität Erlangen-Nürnberg Tag der mündlichen Prüfung: 31.07.2018 Vorsitzender des Promotionsorgans: Prof. Dr. Georg Kreimer Gutachter: Prof. Dr. Lars Nitschke Prof. Dr. Manfred Marschall Table of content Table of content I. Summary ......................................................................................................................... 1 I. Zusammenfassung ......................................................................................................... 3 II. Introduction ..................................................................................................................... 5 1. The human immunodeficiency virus .................................................................................... 5 2. Transposable elements ......................................................................................................... 7 3. Host restriction factors ........................................................................................................ 10 4. The restriction factor SAMHD1 ..........................................................................................
    [Show full text]
  • WO 2016/028843 A2 25 February 2016 (25.02.2016) P O P C T
    (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2016/028843 A2 25 February 2016 (25.02.2016) P O P C T (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every C12Q 1/68 (2006.01) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (21) International Application Number: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, PCT/US20 15/045805 DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (22) International Filing Date: HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, 19 August 2015 (19.08.2015) KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, (25) Filing Language: English PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, (26) Publication Language: English SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (30) Priority Data: 62/039,341 19 August 2014 (19.08.2014) US (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (71) Applicant: PRESIDENT AND FELLOWS OF HAR¬ GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, VARD COLLEGE [US/US]; 17 Quincy Street, Cam TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, bridge, Massachusetts 02138 (US).
    [Show full text]
  • Pharmacokinetics, Pharmacodynamics and Metabolism Of
    PHARMACOKINETICS, PHARMACODYNAMICS AND METABOLISM OF GTI-2040, A PHOSPHOROTHIOATE OLIGONUCLEOTIDE TARGETING R2 SUBUNIT OF RIBONUCLEOTIDE REDUCTASE DISSERTATION Presented in Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy in the Graduate School of The Ohio State University By Xiaohui Wei, M.S. * * * * * * The Ohio State University 2006 Approved by Dissertation Committee: Dr. Kenneth K. Chan, Adviser Adviser Dr. Guido Marcucci, Co-adviser Graduate Program in Pharmacy Dr. Thomas D. Schmittgen Dr. Robert J. Lee Co-Adviser Graduate Program in Pharmacy ABSTRACT Over the last several decades, antisense therapy has been developed into a promising gene-targeted strategy to specifically inhibit the gene expression. Ribonucleotide reductase (RNR), composing of subunits R1 and R2, is an important enzyme involved in the synthesis of all of the precursors used in DNA replication. Over- expression of R2 has been found in almost every type of cancer studied. GTI-2040 is a 20-mer phosphorothioate oligonucleotide targeting the coding region in mRNA of the R2 component of human RNR. In this project, clinical pharamcokinetics (PK), pharmacodynamics (PD) and metabolism of this novel therapeutics were investigated in patients with acute myeloid leukemia (AML). A picomolar specific hybridization-ligation ELISA method has been developed and validated for quantification of GTI-2040. GTI-2040 and neophectin complex was found to enhance drug cellular uptake and exhibited sequence- and dose-dependent down-regulation of R2 mRNA and protein in K562 cells. Robust intracellular concentrations (ICs) of GTI-2040 were achieved in peripheral blood mononuclear cells (PBMC) and bone marrow (BM) cells from treated AML patients. GTI-2040 concentrations in the nucleus of BM cells were found to correlate with the R2 mRNA down-regulation and disease response.
    [Show full text]
  • US5142033.Pdf
    |||||||||||||| USOO5142O33A United States Patent (19) (11) Patent Number: 5,142,033 Innis (45) Date of Patent: Aug. 25, 1992 54) STRUCTURE-INDEPENDENT DNA y AMPLIFICATION BY THE POLYMERASE FOREIGN PATENT DOCUMENTS CHAIN REACTION 0237362 9/1987 European Pat. Off. 75) Inventor: Michael A. Innis, Moraga, Calif. w 025801717 3/19889 European Pat. OffA 73) Assignee: Hoffmann-La Roche Inc., Nutley, OTHER PUBLICATIONS N.J. Barr et al., 1986, Bio Techniques 4(5):428-432. Saiki et al., 1988, Science 239:476-49. (21) Appl. No.: 738,324 Promega advertisement and certificated of analysis 22 Filed: Jul. 31, 1991 dated Aug. 9, 1988 "Tag Track Sequencing System". Heiner et al., 1988, Preliminary Draft. Related U.S. Application Data McConlogue et al., 1988, Nuc. Acids Res. 16(20):9869. 63) continuation of ser, No. 248,556, sep. 23, 1988, Pat. Inset a 1988, Proc. Natl. Acad. Sci. USA No. 5,091,310. 85:9436-9440. & Mizusawa et al., 1986, Nuc. Acids Res. 14(3):1319-1324. 51) Int. C. ....................... C07H 21/04; SES 6. Simpson et al., 1988 Biochem. and Biophys. Res. Comm. 151(1):487-492. 52) U.S. C. .......................................... 536/27; 435/6; Chait, 1988, Nature 333:477-478. 435/15; 435/91; 435/83; 435/810; 436/501; 436/808; 536/28: 536/29; 530/350; 530/820; Primary Examiner-Margaret Moskowitz 935/16: 935/17, 935/18: 935/78; 935/88 Assistant Examiner-Ardin H. Marschel 58) Field of Search ....................... 435/6, 91, 15, 810, Attorney, Agent, or Firm--Kevin R. Kaster; Stacey R. 435/183; 436/501, 808: 536/27-29; 935/16, 17, Sias 18, 78, 88: 530/820, 350 (57) ABSTRACT 56) References Cited Structure-independent amplification of DNA by the U.S.
    [Show full text]
  • WO 2013/188582 Al 19 December 2013 (19.12.2013) P O P C T
    (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2013/188582 Al 19 December 2013 (19.12.2013) P O P C T (51) International Patent Classification: Way, San Diego, California 92122 (US). RONAGHI, Mo- C12Q 1/68 (2006.01) G01N 27/447 (2006.01) stafa; 5200 Alumina Way, San Diego, California 92122 (US). GUNDERSON, Kevin L.; 5200 Illumina Way, San (21) International Application Number: Diego, California 92122 (US). VENKATESAN, Bala PCT/US20 13/045491 Murali; 5200 Illumina Way, San Diego, California 92122 (22) International Filing Date: (US). BOWEN, M. Shane; 5200 Illumina Way, San 12 June 2013 (12.06.2013) Diego, California 92122 (US). VIJAYAN, Kandaswamy; 5200 Illumina Way, San Diego, California 92122 (US). (25) Filing Language: English (74) Agents: MURPHY, John T. et al; 5200 Illumina Way, (26) Publication Language: English San Diego, California 92122 (US). (30) Priority Data: (81) Designated States (unless otherwise indicated, for every 61/660,487 15 June 2012 (15.06.2012) US kind of national protection available): AE, AG, AL, AM, 61/715,478 18 October 2012 (18. 10.2012) US AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, 13/783,043 1 March 2013 (01.03.2013) US BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, (71) Applicant: ILLUMINA, INC. [US/US]; 5200 Illumina DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, Way, San Diego, California 92122 (US). HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, (72) Inventors: SHEN, Min-Jui Richard; 5200 Illumina Way, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, San Diego, California 92122 (US).
    [Show full text]
  • Rapid Isolation of High Molecular Weight DNA from Single Dried
    African Journal of Biotechnology Vol. 11(90), pp. 15654-15657, 8 November, 2012 Available online at http://www.academicjournals.org/AJB DOI: 10.5897/AJB12.1714 ISSN 1684–5315 ©2012 Academic Journals Full Length Research Paper Rapid isolation of high molecular weight DNA from single dry preserved adult beetle of Cryptolaemus montrouzieri for polymerase chain reaction (PCR) amplification Kamala Jayanthi PD1*, Rajinikanth R1, Sangeetha P1, Ravishankar KV2, Arthikirubha A1, Devi Thangam S1 and Abraham Verghese1 1Department of Entomology and Nematology, Indian Institute of Horticultural Research, Hessaraghatta Lake PO, Bengaluru-560089, Karnataka, India. 2Department of Biotechnology, Indian Institute of Horticultural Research, Hessaraghatta Lake PO, Bengaluru-560089, Karnataka, India. Accepted 16 October, 2012 For studying genetic diversity in populations of predatory coccinellid, Cryptolaemus montrouzieri Mulsant (Coccinellidae: Coleoptera), our attempts to isolate high quality DNA from individual adult beetle using several previously reported protocols and even modifications were quite unsuccessful as the insect size was small and was preserved at -20°C as dry specimen. Here we describe a simple, rapid and efficient method of isolating high-quality intact genomic DNA with reduced protein contamination for polymerase chain reaction (PCR) amplification from a single, dry preserved specimen of adult Cryptolaemus. The procedure features macerating and mixing the single adult specimen of Cryptoalemus with cationic detergent cetyltrimethylammonium bromide (CTAB) in the homogenization buffer, two chloroform-isoamylalcohol extractions and an alcohol precipitation. RNA contamination was eliminated with RNAse treatment. The purity of DNA was high since the A260/A280 ratio ranged from 1.78 to 1.97. The isolated DNA was used as template for PCR, and the results were evaluated by comparing with different preserved samples.
    [Show full text]
  • Nucleic Acid Approaches to Toxin Detection Nicola Chatwell
    Nucleic Acid Approaches To Toxin Detection Nicola Chatwell, BSc Thesis submitted to the University of Nottingham for the degree of Master of Philosophy December 2013 ABSTRACT PCR is commonly used for detecting contamination of foods by toxigenic bacteria. However, it is unknown whether it is suitable for detecting toxins in samples which are unlikely to contain bacterial cells, such as purified biological weapons. Quantitative real-time PCR assays were developed for amplification of the genes encoding Clostridium botulinum neurotoxins A to F, Staphylococcal enteroxin B (SEB), ricin, and C. perfringens alpha toxin. Botulinum neurotoxins, alpha toxin, ricin and V antigen from Yersinia pestis were purified at Dstl using methods including precipitation, ion exchange, FPLC, affinity chromatography and gel filtration. Additionally, toxin samples of unknown purity were purchased from a commercial supplier. Q-PCR analysis showed that DNA was present in crudely prepared toxin samples. However, the majority of purified or commercially produced toxins were not detectable by PCR. Therefore, it is unlikely that PCR will serve as a primary toxin detection method in future. Immuno-PCR was investigated as an alternative, more direct method of toxin detection. Several iterations of the method were investigated, each using a different way of labelling the secondary antibody with DNA. It was discovered that the way in which antibodies are labelled with DNA is crucial to the success of the method, as the DNA concentration must be optimised in order to fully take advantage of signal amplification without causing excessive background noise. In general terms immuno-PCR was demonstrated to offer increased sensitivity over conventional ELISA, once fully optimised, making it particularly useful for biological weapons analysis.
    [Show full text]
  • Abbas Thesis
    Thesis for doctoral degree (Ph.D.) 2008 Thesis for doctoral degree (Ph.D.) 2008 Targeting Nucleic Acids in Bacteria with Synthetic Ligands Targeting nucleic acids in bacteria with nucleic synthetic ligands Targeting Abbas Nikravesh Abbas Nikravesh From the Programme for Genomics and Bioinformatics Department of Cell and Molecular Biology Karolinska Institutet, Stockholm, Sweden TARGETING NUCLEIC ACIDS IN BACTERIA WITH SYNTHETIC LIGANDS Abbas Nikravesh Stockholm 2008 All previously published papers were reproduced with permission from the publisher. Published by Kar olinska Institutet. Printed by [name of printer] © Abbas Nikravesh, 2008 ISBN 978-91-7357-489-1 In the Name of God, the Beneficent, the Merciful I would like to dedicate this thesis to my dear late parents Fatemeh and Hassan Questions are the cure for human ignorance. Let us ask what we do not know, and solve our entire unknown (Mohammad Taghi Jafari). ABSTRACT There is a need for new antibacterial agents, and one attractive strategy is to develop nucleic acid ligands that inhibit pathogen genes selectively. Also, such ligands can be used as molecular biology probes to study gene function and nucleic acid structures. In this thesis, bacterial genes were selectively inhibited with antisense peptide nucleic acid (PNA) and the higher order structures formed by (GAA)n repeats were probed with the intercalator benzoquinoquinoxaline (BQQ). A majority of bacterial genes belong to tight clusters and operons, and regulation within cotranscribed genes has been difficult to study. We examined the effects of antisense silencing of individual ORFs within a natural and synthetic operon in Escherichia coli. The results indicate that expression can be discoordinated within a synthetic operon but only partially discoordinated within a natural operon.
    [Show full text]
  • Understanding Nucleic Acid Amplification Techniques in the Detection of Influenza Viruses in Developing Countries REVIEW ARTICLE
    Page 17 Research Article Journal of Research and Review in Science, 17-25 Volume 6, December 2019 DOI:10.36108/jrrslasu/9102/60(0130)REVIEW ARTICLE REVIEW ARTICLE Understanding Nucleic Acid Amplification Techniques in the Detection of Influenza viruses in Developing Countries AbdulAzeez A. Anjorin*1, 2, 3, Olumuyiwa B. Salu2, 3, Robert K. Obi 2, 3, 4, Bamidele O. Oke 2, 3, Akeeb OB. Oyefolu1, Wellington A. Oyibo 2, Sunday A. Omilabu 2,3 1Department of Microbiology (Virology Research), Lagos State University, Ojo, Abstract: Lagos, Nigeria Introduction: Early detection of emerging influenza virus variant is a key factor in the WHO influenza Global strategies for prevention and control. 2 Department of Medical Microbiology and Rapid, accurate, inexpensive and portable detection systems are needed Parasitology, College of Medicine, University of Lagos, Nigeria for influenza virus diagnosis and surveillance. Such a detection system should easily identify all the subtypes of influenza virus. Degenerate 3Virology Research Laboratory, Department primers and probes designed from evolutionally conserved regions for of Medical Microbiology and Parasitology, known influenza A viruses present the best way to identify unknown College of Medicine/ Lagos University Teaching Hospital (LUTH), Idi-Araba, subtypes of influenza A virus by polymerase chain reaction PCR and array Nigeria techniques. The isothermal reactions, Nucleic Acid Sequencing Based Amplification (NASBA) and Loop-mediated isothermal Amplification 4 Department of Microbiology, Federal (LAMP) possess great potential for influenza A virus detection especially University of Technology, Owerri, Imo, Nigeria in developing countries. However, multiplex real-time (rT) or quantitative (q) polymerase chain reaction (qPCR) remains a rapid, accurate and time- Correspondence saving technique used for influenza virus detection.
    [Show full text]
  • View • Inclusion in Pubmed and All Major Indexing Services • Maximum Visibility for Your Research
    Sachla and Eichenbaum BMC Microbiology (2016) 16:68 DOI 10.1186/s12866-016-0687-6 RESEARCH ARTICLE Open Access The GAS PefCD exporter is a MDR system that confers resistance to heme and structurally diverse compounds Ankita J. Sachla and Zehava Eichenbaum* Abstract Background: Group A streptococcus (GAS) is the etiological agent of a variety of local and invasive infections as well as post-infection complications in humans. This β-hemolytic bacterium encounters environmental heme in vivo in a concentration that depends on the infection type and stage. While heme is a noxious molecule, the regulation of cellular heme levels and toxicity is underappreciated in GAS. We previously reported that heme induces three GAS genes that are similar to the pefRCD (porphyrin regulated efflux) genes from group B streptococcus. Here, we investigate the contributions of the GAS pef genes to heme management and physiology. Results: In silico analysis revealed that the PefCD proteins entail a Class-1 ABC-type transporter with homology to selected MDR systems from Gram-positive bacteria. RT-PCR experiments confirmed that the pefRCD genes are transcribed to polycistronic mRNA and that a pefC insertion inactivation mutant lost the expression of both pefC and pefD genes. This mutant was hypersensitive to heme, exhibiting significant growth inhibition already in the presence of 1 μMheme.Inaddition,thepefC mutant was more sensitive to several drugs and nucleic acid dyes and demonstrated higher cellular accumulation of heme in comparison with the wild type and the complemented strains. Finally, the absence of the PefCD transporter potentiated the damaging effects of heme on GAS building blocks including lipids and DNA.
    [Show full text]
  • Molecular Tools for Nucleic Acid Analysis
    Molecular Tools for Nucleic Acid Analysis Deirdre O’ Meara Doctoral Thesis Department of Biotechnology Royal Institute of Technology Stockholm 2001 © Deirdre O’ Meara Department of Biotechnology Royal Institute of Technology, KTH SCFAB 106 91 Stockholm Sweden Printed at Universitetsservice US AB Box 700 14 100 44 Stockholm, Sweden ISBN 91-7283-161-8 Deirdre O’ Meara (2001): Molecular Tools for Nucleic Acid Analysis. Department of Biotechnology, Royal Institute of Technology, KTH, SCFAB, Stockholm, Sweden. ISBN 91-7283-161-8 Abstract Nucleic acid technology has assumed an essential role in various areas of in vitro diagnostics ranging from infectious disease diagnosis to human genetics. An important requirement of such molecular methods is that they achieve high sensitivity and specificity with a fast turnaround time in a cost-effective manner. To this end, in this thesis we have focused on the development of sensitive nucleic acid strategies that facilitate automation and high-throughput analysis. The success of nucleic acid diagnostics in the clinical setting depends heavily on the method used for purification of the nucleic acid target from biological samples. Here we have focused on developing strategies for hybridisation capture of such templates. Using biosensor technology we observed that the hybridisation efficiency could be improved using contiguous oligonucleotide probes which acted co-operatively. By immobilising one of the probes and annealing the second probe in solution, we achieved a marked increase in target capture due to a base stacking effect between nicked oligonucleotides and/or due to the opening up of secondary structure. Such co- operatively interacting modular probes were then combined with bio-magnetic bead technology to develop a capture system for the extraction of hepatitis C RNA from serum.
    [Show full text]
  • Species Diagnostics Protocols PCR and Other Nucleic Acid Methods Series: Methods in Molecular Biology
    springer.com Justin P. Clapp Species Diagnostics Protocols PCR and Other Nucleic Acid Methods Series: Methods in Molecular Biology Species Diagnostics Protocols: PCR and Other Nucleic Acid Methods is premised on the rapid development in recent years of the use of nucleic acid-based technology for the identification of various organisms. The majority of applications have been in such medically related fields as the diagnosis of disease or the identification of dis• ease vectors, but this emphasis is changing as the potential and range of the techniques becomes more generally recognized. This has been especially true since the advent of polymerase chain reaction (PCR) technology. The use of nucleic acids for diagnostic purposes offers numerous advantages over conventional methods of identification. It must be stressed, however, that conventional methods are still extremely valuable and will always remain at the forefront of many diagnostic procedures. The ability of the skilled taxonomist begins to fail when there is a requirement to identify rapidly organisms that are small, immature, or present in large numbers. In such cases the time scale necessary to obtain an identification often precludes practical usage. It is in this context that molecular methods come into their own. There are several advantages that DNA can offer 1996, XI, 416 p. diagnostics other than the obvious and important fact that its base sequence is the fundamental source of biological variation. It offers a great amount of information that is not Printed book accessible to other methods of identifica• tion because the vast majority of DNA is not Softcover expressed. 184,99 € | £162.50 | $229.00 [1]197,94 € (D) | 203,49 € (A) | CHF 218,50 eBook 149,79 € | £130.00 | $179.00 [2]149,79 € (D) | 149,79 € (A) | CHF 174,50 Available from your library or springer.com/shop MyCopy [3] Printed eBook for just € | $ 24.99 springer.com/mycopy Order online at springer.com / or for the Americas call (toll free) 1-800-SPRINGER / or email us at: [email protected].
    [Show full text]