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A revised and enlarged version of Shimazu’s （1988） paper entitled “Trematodes of the Genera , Dimerosaccus and （: Opecoelinae） from freshwater fishes of Japan”

著者名（英） Takeshi Shimazu journal or Journal of Nagano Prefectural College publication title volume 55 page range 15-29 year 2000-12 URL http://id.nii.ac.jp/1118/00000255/

Creative Commons : 表示 - 非営利 - 改変禁止 http://creativecommons.org/licenses/by-nc-nd/3.0/deed.ja JoumalofNaganoPrefecturalCollege，No．55，pp．15－29，December2000 15

ARevisedandEnlargedVersionofShimazu’S（1988）PaperEntitled “TrematodesoftheGenera Coitocaecunq，Dimensaccus and Qt）eCOehts

（Opecoelidae：Opecoelinae）fromFreshwaterFishesofJapan”

TakeshiSHIMAZU＊

Abstract：ThispaperisarevisedandenlargedversionofShimazu’S（1988）paper

entitled“Trematodes of the genera Coitocaecum，Dbnerosaccus and QPecoehLS

（Opecoelidae：Opecoelinae）fromfreshwaterfishesofJapan．”Itdescribesandillus－ tratesthreespeciesinthreegenera（：Opecoelidae）fromfreshwaterfishesof

Japan：Coitocaecum 毎毎如oYChis Ozaki，1926（Opecoelinae）；QPecoehts ukb7iShima－ zu，1988（Opecoelinae）；and DimerosaccL岱 OnCOrhynchi（Eguchi，1931）Shimazu， 1980（Plagioporinae）・Diagnosesofthefamily，Subfamilies，andgeneraareproposed・ SomerelevantdataobtainedsincethepublicationofthepreviouspaperareglVen．

Keyword5：COわCαeC〟刀移 転が0γCねか省略γロぶαcc鋸30乃COγ如乃Cあもq如COgゐ岱 虎なロ玖 Digenea，Opecoelidae，freshwaterfishes，Japan．

In1988I published a paper entitled Cylindrical，aSpinose，nOnOCulate．Oral sucker “Trematodes of the genera Coitocaecum， Subterminal．Prepharynx short or practically

Dimerosaccus and OPecoelus（Opecoelidae： absent．Pharynxpresent．Esophagusvariousin

Opecoelinae）fromfreshWaterfishesofJapan” length．Intestinalbifurcationinforebody．Intes－

（Shimazu，1988b）．Inthisversion，theprevious tineslong，terminating blindly，eaCh opening Paperhasbeenrevisedandenlargedaccording throughananus，unitedtoformacyclocoelor tonewrelevantdataobtainedsinceitspublica－ to open through a common ventralanus，Or tion，andsomemistakesanderrorsfoundinit fusing with excretory vesicle to form a uro－ have been corrected．For the materials and proct．Ventralsuckerinanteriorhalfofbody， methods，the readeris referred to Shimazu SeSSile or pedunculate，with or without papi1－

（1988a）． 1iform or tentacular appendages on anterior andposteriorlipmargins． Fami1yOpecoelidaeOzaki1925 Testesusuallytwo，rarelythreetoten（usu－

allynine），OPpOSite，Oblique，Ortandeminhind－

0pecoelidae Ozaki，1925，p．53（type ， body．Cirruspouchlarge，enClosingwholemale qpgC∂gゐ岱Ozaki，1925）． terminalduct（Orwholeseminalvesicle，prOS－ Coitocaecidae Ozaki，1929，p．76（type genus， taticcomplex，andcirrus）；rudimentary，Small，

CβわCαβαmNicoll，1915）． enclosingsmallinternalseminalvesicle，PrOS－

Podocotylidae Dollfus1960，pp．101－102（type tatic complex，and cirrus，Withlarge external genus，lbdocob，leDujardin，1845）． Seminal vesiclelying freeinparenchyma；Or absent．Genitalatriumsmall．Genitalporeante－

Diqg7WSis．：Digenea．Body small riortoventralsucker，Ventral，uSuallysinistro－ tomedium－Sized，elongateoroval，flattenedor Submedian，rarelymaybemedian，OCCaSionally

With accessory sucker．0vary entire tolobed， ＊物乃口 瑚C加mg C扉極拘．8－49－7 助α， PreteSticular，median or submedian．00type－ 入物乃8380－8525；ノ如α犯． COmplexpreovarian．Laurer’scanalpreovarian， 16 TakeshiSHIMAZU

Openingdorsally．Seminalreceptaclepresentor prostaticcomplex，andcirrus，withlargeexter－ absent；uterineseminalvesiclepresent．Uterus nalseminalvesiclelyingfreeinparenchyma；Or usually preovarian Or preteSticular，rarely absent．Genital pore prebifurcal，OCCaSionally extendingintointer－Or pOSt－teSticular reglOn； With accessory sucker．Seminal receptacle metratermpresent．Eggs operculate，OCCaSion－ absent；uterine seminal vesicle present．Eggs ally with polar filaments，uSually not em－ without polar filaments．Adults parasitic usu－ bryonated or rarely fully embryonated，When allyindigestivetract，rarelyingal1bladder，Of laid．Vitellariafollicular，Circumcecal，COntinu一 marine andfreshwater ．

〇uS Orinterrupted，uSually reaching posterior Dゐcussion．Thissubfamilydiagnosishasbeen end of body and confluentin posトtesticular basedmainlyOzaki（1925b，C，1928），Yamaguti reglOn，Variousin anterior extent．Excretory （1971），GibsonandBray（1982，1984），andthis VeSicletubular，Variousinextent，Withposter0－ Study． terminal or almost dorsal excretory pore．

Adultsparasiticusuallyindigestivetract，rare－ Genus CoitocaeculnNic011，1915 lyin gall bladder，Of teleosts and rarelyin digestivetractofamphibians． Coitocoecum Nicoll，1915，pp．25－26（type species，

Lifb qcle．Miracidia nonoculate，with one CO言わcOgαJ乃7gツ777乃卸如才毎mNicoll，1915）． pairofflamecells；epidermalcellformula6，7， COわCαgCa〝犯：Ozaki，1929，pp．76－77． 4，2．Cotylomicrocercous cercariae developing 0zakiaWigniewski，1934，PP．35，38，tablel（type insporocystsinprosobranchsnai1S；flame－Cell SpeCies，CoitocaecLm〝 吻iorrhisOzaki，1926）． formula2［（2＋2）＋（2＋2）］＝16．Metacercar－ Aeudocoitocaecum Bilqees，1972，P．21（type iae encystinginaquatic arthropods，Snails，Or SpeCies，．托eudocoitocaecZmlihl恵soclesゐ Bilqees， leeches，OCCaSional1yprogenetic． 1972）．

Dゐcwsion．This fami1y diagnosis has been hrYtddcかlostomum Zhukov，1972，p．347（type based main1y on Ozaki（1925b，C，1928）， species，Rlraddc＆lostomumindicumZhukov，1972）． Yamaguti（1971），Gibson and Bray（1982，

1984），Schell（1985），and this study．For the DαgnOSis．0pecoelidae：Opecoelinae．

Classificationofthefamily，thereaderisrefer－ Cyclocoelpresent．Ventralsuckerwithoutmar－ redtoYamaguti（1971）andGibson（1996）． ginal appendages．Cirrus pouch rudimentary， Small，thick－Wallとd，muSCular，enClosing small

SubfamilyOpecoelinaeOzaki，1925 internalseminalvesicle，PrOStaticcomplex，and

Cirrus；eXternalseminal vesiclelarge，freein

0pecoelidae Ozaki，1925，p．53（type genus， parenchyma．Genital pore prebifurcal，rarely

qpecOggZはOzaki，1925）． maybepostbifurcal．Accessorysuckerabsent． 0pecoelinae Stunkard，1931，p．724（type genus， Vitellinefolliclessometimesenteringforebody，

q如COe加Ozaki，1925）． interruptedornotindistribution．Adultspara－ Coitocoecinae Poche，1926（imprint1925），p． Siticnormallyinintestineofmarineandfresh－

163（typegenus，CoitocoecumNicoll，1915）． water teleosts．

Discwsion．Nicoll（1915）established a new

D毎77OSis．Opecoelidae．Intestines ending genus，Coitocoecum，basedonanewspecies，C．

blindly，eaChopeningthroughananuS，unitedto 幻′mn卸halhtm，from a marine ，勒anlS formacyclocoelortoopenthroughacommon ausbulis（Sparidae），from Australia．Ozaki

Ventralanus，Orfusingwithexcretoryvesicleto （1929）changed the original spelling form a uroproct．Ventral sucker sometimes Coitocoecumto Coiiocaec鋸mWithoutexplaining

withmarginalappendages．Testestwo．Cirrus thechange（discussedlateragain）．Sincethen，

pouchrudimentary，Small，thick－Walled，muSCu－ manysubsequentworkershaveusedthiss豆bse－

lar，SurrOundingsmallinternalseminalvesicle， quent spelling．The subsequent spelling TrematodesfromfreshwaterfishesofJapan 17

Coitocaecumisretainedinthispaper． Ozakia 吻ioγChis：Wi喜niewski，1934，p．38，table2．

Nicoll（1915）stated thatin C．Bymn卸hal－

hlm，nO true Cirrus pouchis present，and the 肋ierial examined．1）Lotl．Four（2im一 genitalporeissituatedneartheleftsideofthe mature and 2 gravid）whole－mOuntS（MPM middleofthepharynx．Ozaki（1926，1929）de－ Coll．No．22585）ofC．phqionhisofYamaguti

SCribedandfiguredawell－developedmuscular （1934）foundinintestineofMogumda obscum

Cirruspouchenclosingasmallinternalseminal ［＝OdoTLtObl血 obsclml Dbscu772］（Gobiidae）

VeSicle，PrOStatic complex，and cirrus；and a from Lake Ogura∴Kyoto（not Shiga）Prefec－ largeexternalseminalvesiclelyingfreeinthe ture，On15and30Mayand4June1932． parenchymain C．phqiorchis Ozaki，1926and 2）Lot2．Fifteen（3immatureand12gravid） fourotherspeciesofthegenus．Ozakisaidthe unpublished whole－mOuntS（MPM Coll．No． genitalporeaslateralinthegenericdiagnosis； 22291）of C．phqiorchbinYamaguti，s collec－ butthegenitalporeisdistinctlysubmedian（or tionfoundinintestineof O．0．ObscuクⅦfromthe sublateral）infront oftheleftintestineinthe KatsuraRiver∴Kyoto，On2and5June andlO fivespeciesasinC．Bymn卸halhm官．Wi至niewski July1936．

（1934）proposed a new genus，Ozahia，tO 3）Lot3．ThreegravidwholeィnountS（MPM accommodatethe five species，Separatingit Coll．No．22292）ofC．p吻iorchisofYamaguti fromthegenusCoitocaecumprincipallybythat （1942）foundinintestine of肱obsczml［＝0． the cirrus pouchis short and contains the 0．Obscunl］（experimentalinfection）． ejaculatory duct andinternalseminalvesicle， 4）Lot4．Two gravidwhole－mOuntS（MPM

While an external seminal vesicleis present． Coll・No．22587）ofC．がなiorcゐofYamaguti

However，Crowcroft（1951）andAken’Ovaand （1939）foundinintestine of ChaenogvbiL岱an一 Cribb（1996）proved that C．aJmnqi）hallum 乃〟αγね 鋸γ0αβ乃α［＝C鬼．乙‘γ0才αg乃オα］ also have such a cirrus pouch as seenin C． （Gobiidae）fromLakeBiwa，ShigaPrefecture，

P廟orchねindicating that the two genera as On3December1938． SynOnymS．AccordingtoBray（1987，p．1054，a 5）Lot 5．0neimmature whole－mOunt foot note），the type species of two genera， （NSMT－P13105）ofC．が由iorch怨ofShimazu

乃eudocoitocaecum Bilqees，1972 and fbn－ （1988b）foundbyNagasawainintestineofCh．

ddcかlostomum Zhukov，1972，are the same uniaeniafromLakeBiwaatOnoeon4Febru＿

SpeCies，Whichmeansthatthetwo genera are ary1980． identical；andfurthertheyaresynonymouswith 6）Lot 6．0neimmature whole－mOunt thegenus CoitocaecumJtshouldbementioned， （NSMT－P13106）ofC．phqionhis ofShimazu however，that the submediangenital poreis （1988b）foundinintestine of Ch．urviaenia distinctlypostbifurcalinthetwo genera（Bi1－ fromLakeBiwaatOnoeon6June1980． qees，1972；Zhukov，1972）． 7）Lot 7．Twoimmature whole－mOuntS Thisgenericdiagnosishasbeenbasedmainly （NSMT－P13104）of Ci）ldonhis ofShimazu on Nicoll（1915），Ozaki（1929），Crowcroft （1988b）foundinintestine of Cみ．ゐaza from

（1951），Aken’0va and Cribb（1996），and this LakeBiwa atOnoe on6June1980．

Study． 8）Lot8．Three（1immatureand2gravid）

unidentified and unPublished whole－mOuntS

Cb紀ocαeC比机がαgZorc九ねOzaki，1926 （MPM C011．No．30013）of C．phqiorchis of （Figs．ト4） Shimazu（1988b）in Ozaki，s collection from “SmallGoro”［＝Ch．isazaP］fromLakeBiwa

CoitocoecumblcqionhisOzaki，1926，PP．125－128，nO （otherdatanotgiven）． figure． 9）Lot9．Four（1immatureand3gravid） CoitocaecLmlP吻iorchis：Ozaki，1929，pp．77－82，figs． Whole－mOuntS（MPM Coll．No．22553）of C．

1－3． 1）hqioγChis ofYamaguti（1934）foundinintes－ 18 TakeshiSHIMAZU

Fig∴ト4． CoわCαeα‘犯が智わγCゐゐOzaki，1926．

Figs．1－3．Yamaguti’sunpublishedgravidspecimens（lot2）from Odontobuぬob－

scum obscum from Kyoto．1：Entire worm，Ventral view．2：Terminal

genitalia，Ventralview．3：0variancomplex，dorsalview． Fig．4． ⅠmmatureSpeCimen（lot7）from ChaenogobhLSゐaza from Lake Biwa， terminalgenitalia，Showingathinmembranesurroundingdistalpart of

extemalseminalvesicleandglandcells（prostatic？），Ventralview． （Scalebars：0．5mminFig．1；0．1mminFigs．2－4．） tine of BりtiosZ岱 kawamebaYi［＝Con坤erca foundinintestine of P nudic密S from Lake kawamebari］（Percichthyidae）from the BiwaatOnoeon4May1992．

Asago River，Hyogo Prefecture，On7January 13）Lot13．0neimmature whole－mOunt and7April1932． （NSMT－P13103）of C．phqiorChis ofShimazu

10）Lot10．Twoimmature whole－mOuntS （1988t））found by Nagasawainintestine of （MPM Coll．No．22586）of C．」坤函0γChis of Coiius ohmiensis［＝Co．reinii］（Cottidae） Yamaguti（1939）foundinintestineofPelieoba－ fromLakeBiwaatOnoeon4February1980．

97LS nudicQs（Bagridae）fromLakeBiwa on 14）Lot14．Fourteen（12immatureand2just 7December1938． matured）whole－mOuntS（NSMT－P14615）

11）Lot ll．One gravid whole－mOunt foundinpyloric ceca of Co．rehdifrom Lake

（NSMT－P13102）of C．pltqionh由ofShimazu BiwaatOnoeon4May1992． （1988b）foundbyNagasawainintestineofP Descク旬tion．1）Forthe specimens oflotsl nudic密S from Lake Biwa at Onoe onll and9，4andlO，and3，See Yamaguti（1934，

November1980． 1939，1942），reSPeCtively．Yamaguti（1934）de－

12）Lot12．Twelve（10immatureand2just scribedtheexcretoryvesicleasYMShaped；but

matured）whole－mOuntS（NSMT－P14614） thisorganisdistinctlyI－Shaped，aSdescribedby TrematodesfromfreshwaterfishesofJapan 19

Ozaki（1926，1929）．Inonespecimenoflot3，the Ⅰ－Shaped，eXtendingtoanteriorborderofante－ uterusincludingmanyeggsdescendedalongthe rior testis． left side ofthetestes as far as the posterior Intheimmature specimens oflot7，a thin borderoftheposteriortestis． membrane enclosed the anterior part of the 2）Basedonthespecimensoflots2，5－8，and externalseminalvesicleandglandcells（pros－ l1－14；14gravidwhole－mOuntSmeaSured（Figs． tatic？）；andthemembranewasanteriorlycon－

1－4）．Bodyoblongorlanceolate－Oblong，Slightly nected tothe thickNWalled cirrus pouch but

Widerinhindbodythaninforebody，0．94－1，60 indiscernibleposteriorly（Fig．4）． by O．44－0．78；forebody40－50％of totalbody Discussion．Ozaki（1926）firstdescribedthis length．Oralsuckerantero－terminal，0．12－0．20 SpeCiesas Coitocoecum 毎毎0γCゐwithnofig－ byO．13－0．20．Prepharynxveryshort．Pharynx urefromadultsfoundinthestomachandintes－ globular or elliptical，0．08－0．11in diameter． tine of M．obscura Later Ozaki（1929）rede－ EsophagusO．05－0．12long，bifurcatingmidway SCribedandfiguredthespeciesunderthename betweenpharynxandventralsucker；CyClocoel Coitocaecum pldoYrhねindicating that the ending some distance from posterior end of locality was Saijo，Hiroshima Prefecture， body．Ventralsuckersessile，0．20－0．27byO．20－ Japan；buthedidnotexplainthechangeofthe 0．31；SuCkerwidthratiol：1．44－1．72． Originalspelling Coitocoec乙m3byNicoll（1915）

Testesentire，COntiguous，Oblique，1yingencir－ to C（フォわCαgCを〝ク乞． cledbycyclocoel，0．12－0．23byO．16－0．31．Cir－ Theholotype（No．P．235）ofthespecieswas rus pouch rudimentary，Club－Shaped，thick－ OnCedepositedinthecollectionoftheZoologト

Walled，muSCular，Small，0．12－0．19 by O．04－ CalInstitute，ScienceFaculty，TokyoImperial

0．06，infrontofleftintestine orslightlyover－ University（Ozaki，1926，1929）；butitis

1appingitposteriorly．Internalseminalvesicle believedthattheholotypehasalreadybeenlost small，SWelling，COntainingsperms．Pars pros－ （seeShimazuetal．，1996）．However，therehave taticausuallyovate，aCCOmpaniedbyprostatic been found13slides（MPM Coll．No．30028） cells．Cirrusshort，nOnSPinous．Externalsemi－ 1abeled merely“gori”inJapanese by himin nalvesicle sinuous，rather thick－Walled，ante－ Ozaki’scollection．Theslidesincludespecimens riortoventralsuckerorextendingtomidlevel Ofdigeneans，C．phqiorchis（6immatureand15 orrarelytoposteriorborderofventralsucker， gravidspecimens），ASy2ク坤hylodoru macrvstoma surrounded by dispersed gland cells（pros－ Ozaki，1925（3gravidspecimens），and Genar－ tatic？）．GenitalatriumSmall．Genitalporesub－ Ch噌S怨gpppo Ozaki，1925（1gravidspecimen） median，tOleft of pharynx．0vary globular， （myidentification；SeealsoShimazu，1992，1995 anterO－dextraltolateraltoanteriortestis，0．08p a，b，1997）．Ozaki（1925a，1926，1929）described

O．14in diameter．0viduct usually originated all of these three species on the basis of the fromrightsideofovary．00typelocatedantero－ SPeCimens foundin 肱 obscura from Saijo，

Sinistral to ovary；Mehlis’gland compactly HiroshimaPrefecture（seealso Shimazu，1995 massed around ootype．Laurer’s canallong， b）．It seems certain that the above一mentioned lyingusuallyanteriortoootype，Slightlydilated SpeCimensarepartsofhisoriginalones ofthe at proximalpart（butno sperms seeninthis three species（see Shimazu，1992，1995a，b）． part）．Uterus pre－0Varian or－teSticular，inter－ ThespecimensofC．phqioγChゐmorphologicaト cecal；metraterm Shorter than cirrus pouch． 1yagreewellwithOzaki’S（1926，1929）descripH Eggs rather numerous，56－68by34－38〟min tions forthe species andthe specimens de－ balsam．Vitelline follicles entering forebody SCribedinthispaper． usuallytomidlevelofesophagusorsometimes Yamaguti（1934，1939，1942）described the tolevel of pharynx，Separated anteriorly， SpeCimens oflotsl and9，4andlO，and3， extendingclosetoposteriorendofbody，COnflu－ respectively．Yamaguti（1942）briefly de－ entinpost－teSticularregion．Excretoryvesicle SCribed the species from Misgum鋸ざ a柳il－ 20 TakeshiSIiIMAZU

licaudatus（Cobitidae）from Nisinomiya， Themetacercariahasbeenfoundencystedin

Hyogo Prefecture；and from Gobius similis shrimps：in Leander paucidens［＝nZlaemon

［sensulato］［＝Rhinogvbius sp．］（Gobiidae） Paucidens］（Palaemonidae）from［the Katsu－ fromSirakawa，Kyoto．Hisspecimenshavenot raRiver，Kyoto］（Yamaguti，1942；lexcysted yett）eenlocated． worm，MPM Coll．No．22292）；in Caridh乍a In theimmature specimens oflot7，a thin dg乃fgca‘加α［＝ⅣgOCαγ掃わα de犯行C鋸がα］ membrane surrounded the distalmost part of （Atyidae）near Tosu，Saga Prefecture the externalseminalvesicle and a smallnum－ （Yamaguti and Nisimura，1944；7 excysted ber of gland cells（prostatic？）（Fig．4）．Bray worms and10immature andl gravid wormS

（1987）noted the presence of a similar mem－ recovered from M obscu7u fed metacercariae braneinthe adult of Dacかlosiomum g櫛ihsi experimentally，MPM Coll．No．22293）；N

Bray，1987（Opecoelinae）．The two genera deniicukliafromKatayama，HiroshimaPrefec－

Coiiocaecum and Dacかlosiomum WooIcock， ture（Okabe and Shibue，1952）；and N

1935are closely allied．The presence of the denticuhliafromKurume，FukuokaPrefecture membrane suggests thatthe membraneis the （Shibue，1953）．Yamaguti（1942，lot 3）and VeStigeoftheposteriormembranousportionof Yamaguti and Nisimura（1944）experimen一 the divided cirrus pouch as seeninthe genus 七al1yfedmetacercariaetoM obscuYqfromthe DimensaccusShimazu，1980（givenlater）．The intestineofwhichtheyrecoveredgravidworms Cirrus pouchinitially formed might be of the 20and40dayslater，reSpeCtively．In China， Same type aS thatinthe adult of the genus Komiya and Tajimi（1943）described the

Dわnerosaccus；and the portionlater might metacercariafrom Macrobruchhm官n妙onense

Changein structure with disappearance or （Palaemonidae）in the vicinity of Shanghai． degeneration，1eavingtheanteriorsmallthick－ ThecercariaisnotknowneitherinJapanorin

Walled portion as seenin adults of genera， China．

Coiiocoecum and Qi）eCOehiS Ozaki，1925（given later）．Asto C．phqionhねthemaleterminal Genus OpecoelusOzaki，1925 genitaliain the metacercaria descrit）ed by

Komiya and Tajimi（1943）and Okabe and QPecoehLS Ozaki，1925，pP．51－52（type species， Shibue（1952）issimilartothoseintheadultas qpgc0gお頭血zgガα応Oza軋1925）．

Seeninthispaper．Thechangewouldtakeplace Qt）eCOehLS：Ozaki，1928，pp．6－7． inearlierstagesofdevelopmentofthemetacer－ Qt）卿ter Ozaki，1928，p．17（typespecies，Q留研一 ねγくりαねOzaki，1928）． TheadultofC．phqioγChishasbeenrecorded notonlyfrom Odontobuぬobscuru obscuYubut Diqwsii Opecoelidae：Opecoelinae．Intes－

also from Chaenogt）bhts uroiaeniq Ch．isaza， tinesfusingwitheachothertoopenthrougha “Small Goro”（＝Ch．isazaP），Coreppe7℃a COmmOn Ventral anus near posterior end of

カα甜αmg占αれ CO肋岱 柁ゐ城 〟独和び α柳gJ－ body．Ventralsucker bearingthree pairs of

ggcα〝dαね‘ぶ，ク招gO∂αgr〟∫ 乃富‘dZce蝕 and marginal appendages．Cirrus pouch rudimen－

RhinogDbius sp．from the Kinkiand Chugoku tary，Small，thick－Walled，muSCular，enClosing

districts，Japan（Ozaki，1926，1929；Yamaguti， Smallinternalseminalvesicle，prOStatic com－

1934，1939，1942；Shimazu，1988b；this paper）． plex，andcirrus．Extemalseminalvesiclelarge．

Thespeciesalso occursinChina：inMasiacem－ Accessory sucker absent．Vitelline follicles

behts aczdeatus（Mastacembelidae）in the extending anteriorly to variouslevels，SOme－

Vicinity of Shanghai（Komiya and Tajimi， times entering forebody，Excretory vesicle

1943）andin Sin＊eYra Chuaisi（Percichthy－ reachingto ovary．Adultsparasiticinintestine idae）in Hubei Province（Institute of ofmarine andfreshwater fishes．

Hydrobiology，HdbeiProvince，1973）． Dねcussion．Ozaki（1925b）erectedthisgenus TrematodesfromfreshwaterfishesofJapan 21

with Qi）eCOehLS亜hoeYicus asthetypespecies． whole－mOuntS（3 paratypes，NSMT－P12933

Later Ozaki（1925C）changed the original and3107）of O．uhbri of Shimazu（1988b）

SPelling卸hoericus to申haericwwithout ex－ foundinintestineof Chaenogobiussp．（forthe

Plainingthe change．Since then，many Subse－ middle－reaChes type of Chaenogobius annularl

quentworkershaveusedthissubsequentspell－ 由）（Gobiidae）fromthe Oono Riverin Oono， ing．The subsequent spelling sPhaericusis SOuthernHokkaido，On20and23August1984．

retainedin this paper．Ozaki（1928）created 2）Lot 2．Eighteen gravid whole－mOuntS

another new genus，Qbqgasierl With QPegt2Ster （holotype，NSMT－P13114；17paratypes，3108－ ovata（original1y spelled ovaius）as the type 3113and3115－3117）of O．u物oYi ofShimazu

SpeCies．Manyspecieshavesincebeendescribed （1988b）foundinintestine and rectum（acci－ inbothgenera．Thesetwo genera aresimilar． dental？）of Chaenogobiw sp．（type host）and

Yamaguti（1951）doubted the validity of the Ch．uroiaenia（forthefreshwatertype of Ch．

genus QpqgasteYI According to Ozaki（1928）， annuklrな）fromtheTobetsuRiver（typelocal－ the most striking feature of this species ity）in Kamiiso，SOuthern Hokkaido，On 26

［q夕卿ter OVaia］isthe distribution of the August1984． vitellinefo11icles，Whicharenotconfinedtothe Descr砂tion．Eighteen better－prepared speci－ regiOnposteriortotheventralsucker，aSinthe mensmeasured（measurementsofholotypein

Qt）eCOelus，but extendinto the neck．Cribb parentheses）（Figs．5－10）．Body elongate，

（1985）discussed the differences between the aspinose，1．40－2．90 by O．36－0．70（1．77 by two generainthe protrusion of the ventral O．43）；forebody O．44－0．70（0．53）long，27－ SuCker，thedegreeofdevelopment ofthemar－ 35（30）％oftotalbodylength．Severalpairs

ginal appendages of the ventral sucker，the Ofglandcellspresentinesophagealregion．Oral POSteriorextentoftheseminalvesicle，andthe SuCkerround，Subterminal，0．11－0．17by O．13－ anterior extent of the vitelline follicles；and 0．20（0．12by O．14）．Prepharynx very short． made the QPegaster a synonymOf the genus Pharynx elliptical，0．06－0．08 by O．07－

Qi）eCOelusinconclusion．Iagreewithhim．Not 0．09（0．08indiameter）．Esophagusshort，0．05－ onlyin Qt）eCOelus variabilis Cribb，1985（see 0．16（0．10）long，bifurcating fairly anterior

Cribb，1985）but also in Qpecoelus muhl to ventralsucker；intestines united to open Yamaguti，1940（Yamaguti，1940，MPM Coll． throughasingleventralanusnearposteriorend

No．22135，17gravid whole一mOuntS；See also Of body．Ventral sucker globular，Slightly

Yamaguti，1951）and the digenean to be de－ protruded，locatedataboutjunctionofanterior

scribed below，the vitelline follicles extend and middle one－thirds of body，0．17－0．27by anteriorly to variouslevels from specimen to O．19－0．31（0．21by O．23），with 3 pairs of

SpeCimen evenin a single species：They are finger－Shaped marginal appendages；SuCker distributed to the postbifurcal or bifurcal or widthratiol：1．33－1．73（1：1．72）．

prebifurcallevel．Itispracticallyimpossibleto Testes subspherical，entire or sometimes distinguishthetwogenerafromeachother． Slightlyindentedirregularly，tarldem，1nmiddle Thisgenericdiagnosishasbeenbasedmainly One－third of hindbody，0．10－0．27 by O．20－ onOzaki（1925b，C，1928）andCribb（1985）． 0．43（0．16－0．23by O．21－0．25）．Cirrus pouch rudimentary，thick－Walled，muSCular，Small，

Qpecoelus ukigoriShimazu，1988 0．06－0．10 by O．03－0．04（0．11－0．04），nOt （Figs．5－10） extending posteriorly beyondleftintestine；

internal seminal vesicle short，tubular；ParS

Qpecoehts uhなロ7iShimazu，1988，PP．13－15，figs．8－ PrOStaticasmall，SurrOundedbyasmallnumber 13． Ofprostaticcells；Cirrusshort．Externalseminal VeSicle elongated－Claviform，large，0．24－

Material examinedl）Lotl．Three gravid 0．43（0．31）long，eXtending to posterior bor－ TakeshiSHIMAZU

Fig．5710．Qi）eCOelus u点な07i Shimazu，1988，graVid specimens（lot 2）from

ChaenQgObhlSurOiaenia（formerlythefreshwatertypeofCh．annularis） andChaenogobhlSSp．（formerlythemiddle－reaChestypeofCh．annulaプ庖） from the Tobetsu River，Kamiiso，SOuternHokkaido．5：Entire worm，

holotype，Ventralview．6－7：Anterior part ofbody，ParatyPeS，Showing

anterior extent ofvitellaria，Ventralview．8：Ventralsucker，paratype，

Ventralview．9：Terminalgenitalia，Paratype，Ventralview．10：Ovarian

COmplex，ParatyPe，dorsalview．

（Scalebars：0．4mminFigs．5－7；0．2mminFigs．8－10．）

der of ventral sucker，SurrOunded partly by CeCalfolds；metratermShort，withasphincter． gland cells（prostatic？）．Genitalatrium sma11． Eggs notnumerous，58－64by36－40（62－64by Genital pore sinistro－Submedian，alittle 38－40）pminbalsam．Vitellinefolliclesrather prebifurcal．0vary transversely reniform， Small，endinganteriorlyatfromlevelofante－ median orsubmedian，justpretesticular，0．08－ rior border of ventral sucker to midlevel of

0．16 by O．16－0．35（0．18 by O．19）．00type－ esophagus（att）ifurcallevelinholotype），COn－

COmPlex preovarian．Laurer’s canal running fluentposLtesticularly，rarelywithafewfolli－ transverselybetween ootype and ovary．Semi－ cleslocated dorsal and anterior to ventral nalreceptaclenotseen；uterineseminalrecepta－ SuCker；vitelline reservOir anterior or antero－

Clepresent．UteruSpreOVarian，withafewinter－ dextraltoovary．Excretoryvesiclereachingto TrematodesfromfreshwaterfishesofJapan 23

0Vary；eXCretOry pOre dors0－ Or pOSterO－ rior extentinthe species，aSin0．vaYiabiHs． terminal． Withincreasing the body size，they do not The anterior extent of the vitelline follicles extend as faranteriorlyin thelatter species variedwidely．Outofthe21specimensstudied， （seeCribb，1985）．Thistendencywasnotclear inlO，thevitelline f011icles extendedinto the inthepresentspecies． forebody，butnotreachingthepharyngeallevel； The present trematode was obtained from in7，tOthebifurcallevel；andin3，0nlyjustto thefishcaughtinthestrictlyfreshwaterregiOn theleveloftheanteriormarginoftheventral oftheOonoandTobetsurivers．Allthehitherto sucker（Figs．5－7）． described species ofthe genus Qi）eCOelus（in－

Pnvalence．InJune to August，1984，this Cluding Qt）egaSter），eXCept atleast two，are trematode was found in Ch．urotaenia and parasiticinmarinefishes．Qpecoelus variabilis

Chaenogobius sp．Only from the Oono and COmPletes afreshwaterlife cycleinAustralia

TobetsuriversinsouthernHokkaido：in5（60－ （Cribb，1985）．Manter（1963）reported a 67mmin standard bodylength）of59fish trematodeunderthenameOt＞eCOehiSmuhlfrom

（Chaenogvbius sp．，50－85mm）fromthe Oono freshwater fishesin Fiji，butitslife cycleis River；andin3（80－87mm）of6fish（Ch． unknown．Thereisthepossibilitythatthepres－

uγロiaenia，70－100mm）and7（70－85mm）of14 enttrematodealsoisatruefreshwaterspecies．

fish（Chaenogobiw sp．，70－85mm）from the According toIshino（personal communica－

TobetsuRiver．Thetrematodewasnotfoundin tion），（1）although both types of the host

20fish（Ch．uroiaeniq90－130mm）fromLake fishes are amphidromous，OnCe they have as－

ToronearKushiro，eaSternHokkaido；in3fish Cendedfromsaltto freshwater，theyusually

（Chaenogobius sp．，45－80mm）from the Sho－ neverreturntosaltwateragain；and（2）itis kanbetsuRiverinMashike，CentralHokkaido； COnSidered that theinfected fish（60－87mm） in20fish（ChaenogobゐES Sp．，70－75mm）from Caughtinthetworiversduringthemonths of

theShubunbetsuRiverinMashike；Orin3fish July andAugust，1984，Were2＋or3＋years （ChaenQgObhLSSp．，85－95mm）fromtheChitose Old，Whichmeansthattheymigratedfromsalt RiverinEbetsu，CentralHokkaido． to freshwater duringthemonths ofJuly and Dゐcussion．Thisspeciesismostcloselysimi－ August，198lor1982，0rthattheyhadspentas

larinmorphologyto O．mutu Yamaguti，1940 longastwoorthreeyearsinfreshwater．Most

andO．variabilisCribb，1985．Thespeciesdiffers PreSumably，they acquiredinfection with the fromO．muhtasdescribedbyYamaguti（1940， trematode duringthefreshwaterlife afterthe

1951；the type series，MPM Coll．No．22135） upstreammlgration． fromamarineteleost，Scombr坤Sboqt）S（Scom－ The specieslivesinChaenogobおuniaenia

bropidae），from Hamazima，Mie Prefecture， （formerlythefreshwatertype of Ch．annular－

CentralJapan，mainlyinthattheexternalsemi－ is）andChaenogobh岱SP．（formerlythemiddle－

nal vesicleis shorter，eXtending only to the reaches type of Ch．annular怨）in southern POSterioredgeoftheventralsuckerinsteadof Hokkaido．The elucidation ofthelife cycleis abouthalfwaybetweentheventralsuckerand indeedneeded． theovary．ThespeciesalsoisdifferentfromO． VaYiabilis as described by Cribb（1985）from StibfamilyPlagioporinaeManter，1947 freshwater fishes of several species of the

Brisbane Riversystem，Australia，mainlyin a Plagioporinae Manter，1947，P．286（type genus，

largerbody，aShorterexternalseminalvesicle， P由iqt）OntSStafford，1904）．

longerfinger－Shapedappendagesoftheventral

SuCker，andsmaller eggs（58－64by36－40pm Dicwosis．Opecoelidae．Intestines usually

insteadof67－77by43－48／汀n）． ending blindly，rarely each opening through

Thevitelline follicles were variousinante－ anus，mited to form a cyclocoel or to open 24 TakeshiSHIMAZU

throughacommonventralanus．Ventralsucker Opecoelinae on account of the characteristic

SeSSile or pedunCulate，Without appendages． male terminal genitalia（see also Shimazu，

Testesusuallytwo，rarelythreeto ten．Cirrus 1988b）．Shimazu and Awakura（1993） pouch enclosing whole male terminal duct， removedthegenustothesubfamilyPlagiopor－ entire or dividedinto anterior small thick－ inaebecauseinD．oncoク句nchi，thecirruspouch

Walledandposteriorlargethin－Walledportions． enclosed the whole male terminal genitalia，

Genital pore prebifurcal，uSually submedian， thoughdivided；andtheproximalpartofLaひ rarelymedian．Accessorysuckerabsent．0vary rer，s canalhad a small dilatation，a pOSSible entire tolobed．Seminal receptacle wel1－ VeStigialseminalreceptacle（or a canalicular developed orvestigial；uterine seminalvesicle seminal vesicle），With a small number of usuallyabsent，rarelypresent．Eggsfilamented SPermSinit．The genusis readily separated or not．Vitelline follicles variousin anterior fromthegenusf）J鹿iqi，OntS bythat the cirrus extent．Adults parasiticusuallyinintestine of pouchisdivided（Eguchi，1931，1932；Shimazu， marine and freshwater teleosts and rarelyin 1980，1988b；thispaper）insteadofentire（Staf－

Smallintestineofamphibians． ford，1904；Miller，1940；Shimazu，1990；Gibson， Dゐcussion．Thissubfami1ydiagnosishasbeen 1996）． based chiefly on Manter（1947），Shimazu With regard to the divided cirrus pouch，a

（1990），Gibson（1996），andthisstudy． similar cirrus pouchis presentin Ma7gOlisia が血ね乃血 Bray，1987，月毎画玖Ⅳ描（月毎卸か椚

GenusDimerosaccusShimazu，1980 us）schizoiho7uCiZhang，1992，P（P）allovaris

Zhang，1992，and 勤tervgonoides disaccus

DhneYVSaCCuS Shimazu，1980，pp．163－164（type Zhang，1992（Bray，1987；Zhang，1992）．The SpeCies，AllocYudhlm OnCOプ勿′nChiEguchi，1931）． genus Ma7gOlisia from a marine fish has the intestines united to open througha common

Dicqnosis．Opecoelidae：Plagioporinae．Intes－ ventral anus．Provided that the anatomical

tinesendingblindly．Cirruspouchdividedinto structure of the reproductive systemis tax－

two portions．Eggs not filamented．Vitelline onomically more valuable thanthat of the

follicles occasionally entering forebody．Ex－ digestive systemin this case，it seems to me

CretOryVeSiclereachingovary．Adultsparasitic thatthegenus Mtlrgt）lisiais a member ofthe

inintestineoffreshwaterfishes，rarelyinsmall subfamily Plagioporinae rather than the sub－

intestine ofsalamanders． family Opecoelinae，in which Bray（1987） Dゐcussion．Eguchi（1931）brieflydescribeda placedit，though．P（P）schizothomciandP・

new species，Allocnadium oncoYhynchi（Allo－ （P．）allovarねboth from freshwater fishes do

creadiidae），With no figure．Later Eguchi not belong atleast to the genus P吻iqt）mtS

（1932）gaveafulldescriptionwithfiguresfor owingtothedividedcirruspouch．Theysome－ the species．Peters（1957）reexamined one of what resemble D．oncor勉′nChi（described

Eguchi’Sspecimensandtentativelytransferred below）．However，the former has a4－lobed

thespecies to thegenus PIcqiqi）OntS Stafford， ovary；and thelatter，an0Varywith many

1904（Opecoelidae），butwith some hesitancy prominences．Zhang（1992）assignedthegenus becauseits cirruS pOuCh differs considerably 劫sie叩gDnOides from afreshwaterfishto the

from that of other species of the genus． family Lepocreadiidae Odhner，1905（for the

Yamaguti（1958，1971），Who also reexamined familydiagnosis，SeeGibson，1996）．However， thesyntypeofthespecies，retainedthespecies thegenusshouldbetransferredto thefamily

withinthe genus Allocreadium，Stating thatit Opecoelidaebecause the tegumentis smooth，

ShouldbereferredtoanOpeCOelidgenus．Shima－ becausetheootype－COmplexispreovarian，and

Zu（1980）proposed a new genus，DbneYVSaC－ because the seminal receptacleis absent．It

CuS，based on the speciesin the subfamily differsfromthegenusDimerosaccusinhaving TrematodesfromfreshwaterfishesofJapan 25

theintestinesandvitellinefolliclesbothextend－ 5）Lot 5．Two gravid whole－mOuntS ing posteriorly only to the midlevel of the （NSMT－P13094and3095）ofD．oncoプ句nchiof hindbodyandthediagonaltestesneartheposte－ Shimazu（1988b）foundinintestine of0．m． riorendofthebody． masoufromtheShokanbetsuRiverinMashike， Hokkaido，Onland2August1984．

Dimerosaccus oncorhynchi（Eguchi，193D 6）Lot 6．Two gravid whole－mOuntS Shimazu，1980 （NSMT－P13096and3097）ofD．oncorhynchiof （Figs．1ト13） Shimazu（1988b）foundinintestine of S． leucomaenゐ［＝Sll．leucomaenゐ］from the Allocrudium oncoナ句nchiEguchi，1931，pP．21－22， ShokanbetsuRiverinMashikeon26July1984 nofigure． and2August1984．

Allocreadhlm OnCOYhynchi：Eguchi，1932，pP．24－28， 7）Lot 7．Two gravid whole－mOuntS figs．1－6． （NSMT－P13098and3099）ofD．oncoク句nchiof PILqipponts onco句nchi：Peters，1957，p．140． Shimazu（1988b）foundin rectum（acciden－

Dimervsaccus oncor7ynchi：Shimazu，1980，pp．164－ tal？）of Cothts nozawae（not Cothts Polhα）

166，figs．ト7． （Cottidae）from the Shokanbetsu Riverin P吻ipponiS honshuensis Moravec and Nagasawa， MashikeonlAugust1984． 1998，pp．283－284，fig．1．凡的軋 8）Lot8．Twenty－three（13immatureandlO gravid）whole－mOuntS（NSMT－P14609 and 肋terial examined．1）Lotl．Ten gravid 4610）foundinintestineofSl．phtvhLSfromthe whole，mOuntS（NSMT－P12169－2172）of D． MatsuRiverinHakubaon25and26September OnCO7句nchiofShimazu（1980）foundinintes－ 1993and4April1994． tine of Onco7hnchus祓odunts f．macnstomw 9）Lot9．Fifty－SeVen（39immature and18 l＝0．masouishikawae］（Salmonidae）from gravid）whole－mOuntS（NSMT－P14611and the Nagara River at Gujohachiman，Gifu Pre－ 4612）foundinpyloriccecaandintestineofS．l． fecture，On12September1975，20January1977， PhlVhtsfromNakakurozawa（asmallstream） and31March1979． in Hakuba on5and13September1994，25 2）Lot2．Fiftyイour（18immature and36 November1994，and24May1995． gravid）whole－mOuntS（NSMT－Pl1945－1950 Descr＊tion．1）Shimazu（1980）described and 2168）of D．oncoYhynchi of Shimazu thespecimensoflotsl，2，and4．Reexamination

（1980）foundinpyloric ceca andintestine of Of them hasindicated that the metratermis Sblvelinusleucomaenis phlVius（Salmonidae） Shorterthantheanteriorportionofthe cirrus fromtheSamuRiver（atributary ofthe Chi－ pouch and possesses，arOund the orifice，a kuma River）at Fujisawa，Iiyama，Nagano Sphincter with a crescent－Shaped thickening

Prefecture，On16，17，and24September1978 betweenthecirrusandthemetraterm；andthat， and18March1979． inonespecimqnOflot4，thevitellariareachthe

3）Lot3．0negravidwhole－mOunt（NSMT－ bifurcallevel．Thespecimenoflot3wassimilar

P12173）ofD．onco7勿nchiofShimazu（1980） to themin morphology and measurements． foundinintestineofS．l．PhtviusfromtheHime Shimazu（1980）describedandfigured anoma－ RiverinHakuba，Nagano Prefecture，On13 lous specimensbearingprobablyincompletely July1979． dividedtestesinthespecimensoflot4． 4）Lot4．Twelve（4immatureand8gravid） 2）Basedonthespecimensoflots5－7（Figs． whole－mOuntS（MPM Coll．No．19260）of D． 11－13）．BodylanCeOlate－Oblong，SOmetimes OnCOク句′nChiofShimazu（1980）foundinintes－ Withtruncateposteriorend，1．36－2．30byO．50－ tine of O．masou f．ゐhikawail＝0．masou 0．78；forebody34－44％ of total bodylength． masou］fromtheHoreiRiverinSanriku，Iwate Oralsucker O．16－0．20by O．16－0．21．Prephar－

Prefecture，On19March1978． ynx very short．Pharynx O．1ト0．16by O．11－ 26 TakeshiSHIMAZU

0．15．EsophagusO．12－0．20long．Intestinalceca 1980）．Shimazu（1980）described the speci－ extendingto nearposterior end ofbody．Ven－ mens oflots1－4，and Shimazu（1988b）reex－ tralsucker sessile，pOStbifurcal，0．24－0．29by amined them and described the specimens of O．26－0．32；SuCkerwidthratiol：1．39－1．71． lots5－7．ShimazuandAwakura（1993）report－

Testes entire orslightlyindented，inmiddle ed adults of the species from 0．m．masou One－thirdofhindbody，0．11－0．27byO．20－0．35． caughtatsea．MoravecandNagasawa（1998）

Anteriorportion of cirruspouchthick－Walled， proposedanewspecies，Pkqi卸OniS honshuen一 muscular，Sma11，0．08－0．13by O．05－0．06，en－ 泡onthebasisofthespecimensfoundinthe Closingveryshortdistalpartofseminalvesicle， intestine of Liobqg7W reini（Amblycipitidae）

Smallparsprostatica，aSmallnumberofpros－ fromthe Sho River at Ohta，Toyama Prefec－ tatic cells，and short cirrus；pOSterior portion ture，Japan．Myreexamination ofthreepara－ thin－Walled，membranous，large，0．16－0．30long， types of this species（NSMT－P15026－5028） not extending posteriorly t）eyOnd midlevel of hasshownthatthespeciesis conspecificwith

Ventral sucker，enClosing undulating tubular D．oncoプ句nchi．Many worms of the species proximalpart of seminalvesicle and alarge werefoundintheintestineofCo．polhαandSl number of gland cells（prostatic？）．Gemital l．PhtviusfromtheIdeRiveratAraya，Iiyama， atriumSmall．Genitalpore sinistro－Submedian On24SeptemberandlOctober1995，reSpeCtive－ atpharyngeallevel．0varytransverselyrenifom， 1y；butthesespecimenshavealreadybeenlost triangular，Or Weakly trilobed，0．09－0．16by （myunpublisheddata）．

O．16－0．24．00tyPe－COmPlexanterior oranter0－ ThespecimensfromHonshu（lots1－4，8，and

1ateraltoovary．Laurer’scanalhavingasmall 9；Shimazu，1980，figs．l and3；and those of dilatationtocontainasmallnumberofsperms MoravecandNagasawa，1998）andthosefrom andovaatproximalpart，Openingdorsallyto Hokkaido（lost 5M7）agree well with each

OVary．Uteruspreovarian，intercecal；metratem otherbut differin anterior extent ofthevite1－

Well－developed，Shorterthananteriorportionof 1inefollicles．Intheformer（Honshuform），the

Cirruspouch；Sphincterbearingacrescentthick－ vitellinefollicles endposteriorto thebifurcal ening．Eggs about20，58－64by34－38ノJmin level；butinthelatter（Hokkaidoform），ante－ balsam．Vitellariaextendinganteriorlytomid－ rior toit（see also Shimazu，1988b；Shimazu

1evelofesophagus．Excretoryvesiclereaching andAwakura，1993；Shimazu，1994）．Itis not

OVary． certainasyetwhetherthis differenceissuffi－

There was not seen a clearCut boundary cientlysignificanttodistinguishspecies． betweenthetwoportionsofthecirruspouchin Theadultstageofthespecieshassofarbeen

SOme SPeCimens（Fig．12）．The cirrus pouch recorded from Cothts nozawae，Co．pol如 was constricted and at this constrictionit was 上gO∂曙門帯 磁抑え 0乃Cの勿乃Cゐぴ 肌がβぴ ゐゐ戊α－ dividedinto thetwo portions．The anatomical Ⅷαち 0．刑．∽お粥 助g〃g肋MJe〟CO∽αe乃ゐ

StruCtureOfthecirruspouchappearedtotrans－ teucomaenねand Sll．Phtvius from the Hok－ formgraduallyfromthemusculartothemem－ kaido，Tohoku，and Chubu districts，Japan

（Eguchi，1931，1932；Shimazu，1980，1988b；this 3）Thespeqimensoflots8and9weremoト paper）．Inaddition，OnegraVidworm（NSMT－ Phologicallyslmilartothoseoflots1－4． P14613）oftheHonshuformwasfoundinthe Discussion．Eguchi（1931，1932）described smallintestine of the salamander Oプ砂－ thisspecies asA．oncoク勿nchifromthe diges－ chodac＆hlS j＊onicus（Amphibia：Caudata： tivetract（mostlytheintestine）of Oncoク句′n－ Hynobiidae）from Nakakurozawain Hakuba Cゐ描 ∽αCmSね∽ぴ［＝0．桝が0〟 なあ戊α紺αβ］ on25November1994（myunpublisheddata）． fromtheNagaraRiver（theexactlocalitynot Thelifecycleremainsunknown．

SpeCified）．Probably，all of Eguchi’s original

SpeCimens have already beenlost（Shimazu， TrematodesfromfreshwaterfishesofJapan 27

Fig．11－13．Dbnerosaccus oncorhynchi（Eguchi，1931）Shimazu，1980，graVid speci－

mens（lot5）from OncoYhynchus masou masou from the Shokanbetsu

River，Mashike，Hokkaido．11：Entireworm，Ventralview．12：Terminal

genitalia，Ventralview．13：0variancomplex，dorsalview． （Scalebars：0．5mminFig．11；0．1mminFigs．12－13．）

Acknowledgments 2）Bilqees，F．M．（1972）：Marinefishtrematodes OfWestPakistanVIII．Descriptionofthirteen Ithank Prof．TeijiKifune，Fukuoka University， new species including a new genus Fukuoka，forhelpfulsuggestion onthe nomenclaH Aeudocoitocaecum fromfishes ofKarachi．1n： ture．Themostpartofthetextandallofthefigures Helminth Parasit．es of Some Vertebrates Ofthepreviouspaperhavebeenreproducedinthis ChieflyfromFishesofWestPakistan（F．M． paperbypermissionoftheEditoroftheJapanese Bilqees，R．Saeed，R．Rehana，A．Khatoon，and SocietyofSystematicZoology． S．H．Kaikabad，eds．），pp．1－40，Agricultural Research Council，Government of Pakistan， References Karachi． 3）Bray，R．A．（1987）：Somehelminthparasites D Aken’0va，T．0．andCribb，T．H．（1996）：Two Of marine fishes of South Africa：family speciesofCoitocaecumNicoll，1915（Digenea： Opecoelidae（Digenea）．J．Nat．Hist．，21，1049－ Opecoelidae）fromMoretonBay，Queensland， 1075． Australia．Syst．Parasito1．，33，217－224． 4）Cribb，T．H．（1985）：Thelifecycleandbiology 28 TakeshiSHIMAZU

Of Oi）eCOehiS VaYiabilis，Sp．nOV．（Digenea： trematodesoffishesofFiji．II．FamiliesLepo－ Opecoelidae）．Aust．J．Zoo1．，33，715－728． Creadiidae，Opistholebetidae，andOpecoelidae．

5）Crowcroft，P．W．（1951）：Notesonthetaxon－ J．Parasito1．，49，99－113． Omy Of the genus Coitocaecum Nicol1， 16）Miller，M．J．（1940）：Parasites offreshwater

1951（Digenea：Opecoelidae）．J．Parasito1．，37， fish．ⅠII．Further studies on the internal 251－256． trematodesoffishinthecentralSt．Lawrence 6）DollfuS，R．Ph．（1960）：Recherches expgr－ WaterShed．Can．J．Res．，Sect．D，18，423－434． imentales sur Nicolla Btlllica（R．・Ph．Dollfus 17）Moravec，F．and Nagasawa，K．（1998）：He1－ 1941）R．一Ph．Dollfus1958，Sa CerCaire minth parasites ofthe rare endemic catfish， COtylicerqueetsam邑tacercaireprog∈n芭tique． LiobagntS rehl乙inJapan．FoliaParasitolり45， Observations surla famille des Coitocaecidae 283－294． Y．Ozaki1928，S．f．CoiiocaecinaeF．Poche1926 18）Nicoll，W．（1915）：Thetrematodeparasitesof

7bmatoddPodocoかloidea etsurles cercaires North Queensland．ⅠⅠⅠ．Parasites of fishes． COtylicerques d’eau douce et marines．Ann． Parasitology，8，22－41，pls．4－5． Parasito1．，35，65－117． 19）Okabe，K．and Shibue，H．（1952）：On On－ 7）Eguchi，S．（1931）：［On anewspecies ofthe COmehmia nosqphonlRobsonandtwo species trematode genus Allocreadhlm ParaSiticin of metacercaria［sic］found in the fresh－ Onco吻／nChus macrostomus．］Nihon Kisei－ water shrimp，A匂ocaridhw denticulaia（de ChugakkaiKiji，No．3，20－22．（InJapaneSe．） Haan）collectedin Katayama，Hiroshima

8）Eguchi，S．（1932）：Studies onsomeparasites Prefecture．（Studies on the control of schis－ OfOncoYhynchusinJapan．Ⅰ．Anewtrematode tosomiasisjaponicaIV．）KurumeIgakkaiZas－ from O乃COク句′乃Cゐ描∽αcmぶわ∽描Or“amagO．” shi，15，607－611．（InJapanese，With English Osaka KotoIgaku Semmongakko Zasshi，1， Summary．） 24－29，1pl． 20）Ozaki，Y．（1925a）：On a new genus of fish 9）Gibson，D．I．（1996）：Trematoda．1h：Guideto trematodes，GenaγChqPめandanewspeciesof the Parasites of Fishes of Canada（L Mar A印画勿′わ血相．Jpn．J．Zoolり1，101－108． golis and Z．Kabata，eds．），PartIV，NRC 21）Ozaki，Y．（1925b）：Preliminary notes on a ResearchPress，Ottawa，373pp． trematodewith anus．J．Parasito1．，12，51753，

10）Gibson，D．I．andBray，R．A．（1982）：Astudy pl．7． and reorganization of Pl庭iqPonLS Stafford， 22）Ozaki，Y．（1925C）：Preliminary notes on a 1904（Digenea：Opecoelidae）andrelatedgen－ trematodewithanus．DobutsugakuZasshi，37， era，Withspecial reference to forms from 416－423，P1．8．（InJapanese，withEnglishsum－ European Atlantic waters．J．Nat．Hist．，16， mary．） 529－559． 23）Ozaki，Y．（1926）：［On some new species of 11）Gibson，D．I．and Bray，R．A．（1984）：On trematodes of freshwater fishes fromJapan Aプ70∽αわかで∽α Zhukov，1957，PgJJα77秒ZO乃 （Preliminary report）．］Dobutsugaku Zasshi， Montogomery，1957，and〔砂ecoelina Manter， 38，124－130．（InJapanese．） 1934（Digenea：Opecoelidae），With a descrip－ 24）Ozaki，Y．（1928）：On some trematodes with

tion of Anomaloわでma koiae sp．nov．from anus．Jpn．J．Zoo1．，2，5－33． NorthAtlanticwaters．J．Nat．Hist．，18，949－ 25）Ozaki，Y．（1929）：Note on Coitocaecidae，a 964． newtrematodefamily．Annot．Zool．Japon．，12， 12）Institute of Hydrobiology，Hubei Province 75－90． （1973）：［AnIllustratedGuidetoFishDiseases 26）Peters，L．E．（1957）：An analysis of the and Pathogenetic Faunaand Florain Hubei trematodegenusAllocreadiumLoosswiththe Province．］KexueChubanshe，Beijing，456pp． description of Allocreadium neotenicum sp． （InChinese．） nov．fromwaterbeetles．J．Parasito1．，43，136－ 13）Komiya，Y．and Tajimi，T．（1943）：Metacer． 142． Cariae from 〟αCγ0γαCゐ〝名吟 乃坤∂朋がね 27）Poche，F．（1925，issued1926）：DasSystemder ［sic］（de Haan）inShanghaiarea andtheir Platodaria．Arch．Naturgesch．，Abt．A，91，1－ excretorysystem．ShanghaiSizenkagakuKen－ 458，pls．ト7． kyushoIho，13，45－57，pls．1－4．（InJapanese， 28）Schell，S．C．（1985）：HandbookofTrematodes withEnglishsummary．） of NorthAmerica Northof Mexico．Univer－ 14）Manter，H．W．（1947）：The digenetic SityPressofIdaho，Moscow，263pp． trematodesofmarinefishesofTortugas，Flor－ 29）Shibue，H．（1953）：Onsometrematodelarvae ida．Am．Midl．Natura1．，38，257－416． foundinthefresh－WaterShrimp，Neocaridina 15）Manter，H．W．（1963）：Studies on digenetic denticulaia（de Haan）collected in Kurume TrematodesfromfreshwaterfishesofJapan 29

District∴Kyushu∴KurumeIgakkaiZasshi，16， OnCO7句′nCki（Opecoelidae），inmasu salmon 780－790．（InJapanese，With English sum－ （Oncoク句′nChus masou masou）caught atsea． mary．） Sci．Rep．HokkaidoFishHatchery，No．47，1－

30）Shimazu，T．（1980）：Dimerosaccus gen．nov． 5． （Digenea：Opecoelidae），witha redescription 41）Stafford，J．（1904）：Trematodes from Ofitstypespecies，Dimervsaccus oncoYhynchi Canadianfishes．Zool．Anz．，27，481－495． （Eguchi，1931）comb．nov．Jpn．J．Parasito1．， 42）Stunkard，H．W．（1931）：Further・Observations 29，163－168． OftheoccurreneofanalopenlngSindigenetic 31）Shimazu，T．（1988a）：Trematodesofthegenus trematodes．Z．Parasitenkd．，3，713－725． Allocreadium（Allocreadiidae）from fresh－ 43）WiSniewski，L W．（1934）：Beitrag zur Water fishes ofJapan．Bull．Natl．Sci．Mus．， Systematik der Coitocaecidae（Trematoda）． Tokyo，Ser．A，14，1－21． ∧樗coJ肋 g．n”Ozα烏ぬ g，nリ CofわCαgCaf肌 32）Shimazu，T．（1988b）：Trematodesofthegen－ Proavitumsp．n．M∈m．Acad．Polon．Sci．Lett．， era Coitocaecum，Dimerosaccus and Oi）eCOelus Cracovie，Cl．Sci．Math．Nat．，Ser．B，No．6，27－

（Opecoelidae：Opecoelinae）from freshwater 41． fishesofJapan．Proc．Jpn．Soc．Syst．Zoo1．，No． 44）Yamaguti，S．（1934）：Studiesonthehelminth 37，1－19． faunaofJapan．Part2．Trematodesoffishes，Ⅰ． 33）Shimazu，T．（1990）：Trematodes of a new Jpn．J．Zoo1．，5，249－541． genus・NeqPl昭iopontf gen・n・（Digenea： 45）Yamaguti，S．（1939）：Studiesonthehelminth Opecoelidae：Plagioporlnae），andanunidenti－ faunaofJapan．Part26．Trematodesoffishes， fiedopecoelidfromfreshwaterfishesofJapan． VI．Jpn．J．Zoo1．，8，211－230，pls．29－30． Jpn．J．Parasito1．，39，384－396． 46）Yamaguti，S．（1940）：Studiesonthehelminth 34）Shimazu，T．（1992）：Trematodesofthegenera faunaofJapan．Part31．Trematodesoffishes， A即頑句心血叩，A明如血狛励め and fbぬβ抑 ⅤⅠⅠ．Jpn．J．Zoo1．，9，35－108，pls．1－2． Chゐ（Digenea：Lissorchiidae）from fresh－ 47）Yamaguti，S．（1942）：Studiesonthehelminth Waterfishes ofJapan．J．Nagano Pref．Coll．， faunaofJapan．Part39．Trematodesoffishes No．47，1－19． mainlyfromNaha．Trans．Biogeogr．Soc．Jpn．， 35）Shimazu，T．（1994）：Adult digenetic 3，329－398，pl．24． trematodes parasiticin freshwater fishes of 48）Yamaguti，S．（1951）：Studiesonthehelminth Hokkaido，Japan：a reView．Sci．Rep．Hok－ faunaofJapan．Part44．Trematodesoffishes， kaidoFishHatchery，No．48，69－78． IX．Arb．Med．Fak．Okayama，7，247－282，pls． 36）Shimazu，T．（1995a）：Trematodesofthegenus 1－5． Genarchopsis（Digenea，Derogenidae， 49）Yamaguti，S．（1958）：The Digenetic Halipeginae）fromfreshwaterfishesofJapan． Trematodes ofVertebrates，Partl．Systema Proc．Jpn．Soc．Syst．Zoo1．，No．54，1－18． Helminthum，Vol．1，pp．1－979．Interscience 37）Shimazu，T．（1995b）：Arevisedchecklistand Publishers，NewYork． bibliography ofthe platyhelminthparasites 50）Yamaguti，S．（1971）：Synopsis of Digenetic reportedbyDr．Yoshimasa Ozaki，1923－1966， Trematodes ofVertebrates．2voIs．，1074pp．， andtheirspecimens depositedintheMeguro 349pls．，KeigakuPublishing，Tokyo． Parasitological Museum，Tokyo．J．Nagano 51）Yamaguti，S．and Nisimura，H．（1944）：One Pref．Coll．，No．50，33－50． nematode and two trematodelarvae from 38）Shimazu，T．（1997）：Cestodes of freshwater Cbridina deniicuhlia de Haan．Hukuoka Acta earthwormsandfreshwaterfishesinJapan：a Medり37（6），ト6，51． review．J．NaganoPref．C011．，No．52，9－17．（In 52）Zhang，T．・f．（1992）：Parasitictrematodesfrom Japanese，withEnglishabstract．） fishes of Sichuan Provincein ChinaII．One 39）Shimazu，T．，Araki，J．，and Kamegai，S． new genus and four new species of

（1996）：Further notes on the platyhelminth Urotrematidae，Opecoelidae and Lepo－ parasitesreportedbyYoshimasaOzaki，1923－ Creadiidae（Trematoda：Digenea）．Acta 1966，withalistofhelminthparasitespecimens Zootaxonom．Sinica，17，6－15．（In Chinese， depositedintheDepartment ofZoology，Uni－ WithEnglishsummary．） VerSityMuseum，UniversityofTokyo，Tokyo． 53）Zhukov，E．Ⅴ．（1972）：New genera of J．NaganoPref．Coll．，No．51，11－15． trematodes from marine fishes ofIndia．Par－ 40）Shimazu，T．andAwakura，T．（1993）：Occur－ azitologiya，6，346－350．（InRussian，withEng－ renceofafr’eShwaterdigenean，Dbnensaccus 1ishsummary．）