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Genome Editing with CRISPR/Cas9 in Postnatal Mice Corrects PRKAG2 Cardiac Syndrome
Cell Research (2016) 26:1099-1111. © 2016 IBCB, SIBS, CAS All rights reserved 1001-0602/16 $ 32.00 ORIGINAL ARTICLE www.nature.com/cr Genome editing with CRISPR/Cas9 in postnatal mice corrects PRKAG2 cardiac syndrome Chang Xie1, 2, *, Ya-Ping Zhang3, *, Lu Song2, *, Jie Luo1, Wei Qi2, Jialu Hu3, Danbo Lu3, Zhen Yang3, Jian Zhang2, Jian Xiao1, Bin Zhou4, Jiu-Lin Du5, Naihe Jing2, Yong Liu1, Yan Wang1, Bo-Liang Li2, Bao-Liang Song1, Yan Yan3 1Hubei Key Laboratory of Cell Homeostasis, College of Life Sciences, Wuhan University, Wuhan 430072, China; 2The State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China; 3Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China; 4Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sci- ences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue-Yang Road, Shanghai 200031, China; 5Institute of Neuroscience and State Key Laboratory of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Acade- my of Sciences, 320 Yue-Yang Road, Shanghai 200031, China PRKAG2 cardiac syndrome is an autosomal dominant inherited disease resulted from mutations in the PRK- AG2 gene that encodes γ2 regulatory subunit of AMP-activated protein kinase. Affected patients usually develop ventricular tachyarrhythmia and experience progressive heart failure that is refractory to medical treatment and requires cardiac transplantation. In this study, we identify a H530R mutation in PRKAG2 from patients with famil- ial Wolff-Parkinson-White syndrome. By generating H530R PRKAG2 transgenic and knock-in mice, we show that both models recapitulate human symptoms including cardiac hypertrophy and glycogen storage, confirming that the H530R mutation is causally related to PRKAG2 cardiac syndrome. -
20120906-Ob-Umbrella.Pdf
U m b r e l l a By the same author F ICTION The Quantity Theory of Insanity Cock and Bull My Idea of Fun Grey Area Great Apes The Sweet Smell of Psychosis Tough, Tough Toys for Tough, Tough Boys How the Dead Live Dorian Dr Mukti and Other Tales of Woe The Book of Dave The Butt Liver Walking to Hollywood N on- F ICTION Junk Mail Sore Sites Perfidious Man Feeding Frenzy Psychogeography (with Ralph Steadman) Psycho Too (with Ralph Steadman) U m b r e l l a W i l l S e l f First published in Great Britain 2012 Copyright © 2012 by Will Self The moral right of the author has been asserted No part of this book may be used or reproduced in any manner whatever without written permission from the Publishers except in the case of brief quotations embodied in critical articles or reviews ‘Apeman’ by Ray Davies © Copyright 1970 Davray Music Ltd. All rights administered by Sony/ATV Music Publishing. All rights reserved. Used by permission ‘Chirpy Chirpy Cheep Cheep’ (Cassia/Stott) © 1971 Warner Chappell Music Italiana Srl (SIAE). All rights administered by Warner Chappell Overseas Holdings Ltd. All rights reserved ‘Don’t Let It Die’ (Smith) – RAK Publishing Ltd. Licensed courtesy of RAK Publishing Ltd. ‘Sugar Me’ by Barry Green and Lynsey De Paul © Copyright Sony/ATV Music Publishing Ltd. All rights reserved. Used by permission ‘Take Me Back to Dear Old Blighty’ Words and Music by Fred Godfrey, A. J. Mills & Bennett Scott © 1916. Reproduced by permission of EMI Music Publishing Ltd, London W8 5SW Every reasonable effort has been made to trace copyright -
Ascential Secure
Ascential Secure Enhanced Health & Safety Standards at Ascential’s Events in a Post-COVID-19 World 1 About Ascential Secure Ascential Secure is our approach to enhanced health and safety standards at our events following COVID-19. From exhibitors to sponsors, speakers, visitors and journalists, those who come to our events do so to gain the information, insights, connections, data and digital tools that they need, effectively and safely. The standards and practices that make up Ascential Secure are designed to provide confidence that at every Ascential event, we are striving to provide the highest standards of safety, hygiene, cleanliness and quality. Whether they are exhibitors, attendees, visitors, speakers or sponsors, people come to events to connect, learn, know more and do more business, effectively, safely and with confidence. Ascential Secure is based on a set of event industry standards, called AllSecure. The AllSecure industry standards were developed collaboratively by a group including industry associations UFI, AEO and SISO, event organisers Informa, Reed Exhibitions and Clarion, a range of event venues, suppliers and with input from health, government and local authorities. Ascential Secure is the way these industry-wide AllSecure standards are being applied to Ascential live events. You can expect to see that health and safety continues to be a priority, and that a range of measures are in place to ensure everyone involved is able to enjoy a safe, hygienic, productive and high-quality organised event experience. As a starting point, Ascential Secure events will be run according to the guidance of the government or official local authority for that location, and according to any venue-specific regulations. -
Annual Report 2020
In pursuit of progress since Annual report 2020 report Annual Annual report 2020 In pursuit of progress since Annual report 2020 report Annual Annual report 2020 CONTENTS ANNUAL REPORT STRATEGIC REPORT 2 Five-year summary 3 Group overview 4 From the chairman 6 From the chief executive 8 From the editor 9 Business review: the year in detail 13 The Economist Educational Foundation 15 The Economist Group and environmental sustainability 17 Corporate governance: the Wates Principles, our Section 172(1) statement and our guiding principles REPORT AND ACCOUNTS GOVERNANCE 22 Directors 23 Executive team 24 Trustees, board committees 25 Directors’ report 28 Directors’ report on remuneration 31 Financial review CONSOLIDATED FINANCIAL STATEMENTS 35 Independent auditor’s report to the members of The Economist Newspaper Limited 38 Consolidated income statement 39 Consolidated statement of comprehensive income 40 Consolidated balance sheet 41 Consolidated statement of changes in equity 42 Consolidated cashflow statement 44 Notes to the consolidated financial statements COMPANY FINANCIAL STATEMENTS 94 Company balance sheet 95 Company statement of changes in equity 96 Notes to the company financial statements NOTICES 108 Notice of annual general meeting 1 STRATEGIC REPORT Five-year summary 2020 2019 2018 2017 2016 £m £m £m £m £m Income statement—continuing business* Revenue 326 333 329 303 282 Operating profit 31 31 38 43 47 Profit after taxation 21 25 28 39 37 Profit on sale of CQ-Roll Call, Inc - 43 - - - Profit on sale of Economist Complex - - - - -
Annual Report 2012
BRINGING How we have performed KNOWLEDGE FIN ANCIAL HIGHLIGHTS • Record adjusted diluted EPS up 7.7% to 40.7p (2011: 37.8p), ahead of market expectations TO LIFE • Full year dividend increased by 10.1% – second interim dividend of 12.5p giving a total 2012 dividend of 18.5p (2011: 16.8p) Businesses, professionals and • Revenue broadly flat despite Robbins Gioia and European academics worldwide turn to Informa Conference disposals – £1.23bn (2011: £1.28bn) for unparalleled knowledge, up-to- • Adjusted operating profit up 4.0% to £349.7m the minute information and highly (2011: £336.2m); organic growth of 2.8% specialist skills and services. • Record adjusted operating margin of 28.4% (2011: 26.4%) Our ability to deliver high quality • Adjusted profit before tax of £317.4m up 7.3% (2011: £295.9m) knowledge and services through • Statutory profit after tax of £90.7m (2011: £74.3m) multiple channels, in dynamic and rapidly changing environments, • Strong cash generation – operating cash flow up 5.7% to £329.0m (2011: £311.2m) makes our offer unique and • Balance sheet strength maintained – net debt/EBITDA extremely valuable to individuals ratio of 2.1 times (2011: 2.1 times) and organisations. OPEAI R T ONAL HIGHLIGHTS • Proactive portfolio management drives significant Annual Report & Financial Statements for the year ended December 31 2012 improvement in the quality of Group earnings • Total product rationalisation reduced Group revenue by 2% • Investment in new products, geo-cloning and platform development • Acquisition of MMPI and Zephyr -
Strategic Orientation Document for Malaria Vector Surveillance and Control in Latin America and the Caribbean
Amazon Malaria Initiative/ Amazon Network for the Surveillance of Antimalarial Drug Resistance Strategic Orientation Document for Malaria Vector Surveillance and Control in Latin America and the Caribbean INICIATIVA AMAZÓNICA CONTRA LA MALARIA/ RED AMAZÓNICA DE VIGILANCIA DE LA RESISTENCIA A LOS ANTIMALÁRICOS Documento estratégico para la gestión del suministro y garantía de la calidad de los medicamentos e insumos para el diagnóstico y tratamiento de la malaria Recommended Citation: US Centers for Disease Control and Prevention (CDC), Pan American Health Organization (PAHO/WHO), and RTI. 2011. Strategic Orientation Document for Malaria Vector Surveillance and Control in La tin America and the Caribbean. Submitted to the U.S. Agency for International Development by the CDC and Links Media, LLC. Gaithersburg, MD. Available at http://www.usaidami.org/resources.shtml Amazon Malaria Initiative/ Amazon Network for the Surveillance of Antimalarial Drug Resistance TABLE OF CONTENTS 1. Glossary / 5 2. Acronyms and abbreviations / 6 3. Objective / 7 4. Introduction / 9 5. Vector control / 11 5.1 Indoor residual spraying / 12 5.2 Insecticide-treated nets / 13 5.3 Source reduction / 14 5.4 Integrated vector management / 15 6. Vector surveillance / 17 6.1 Entomological surveillance / 18 6.2 Monitoring vector control operations / 22 6.3 Entomological indicators / 23 6.4 Operational indicators / 25 7. Monitoring plans per epidemiological strata / 29 7.1 Moderate to low-transmission settings / 29 7.2 Low to very low-transmission settings / 31 7.3 Settings with no active transmission but risk of transmission / 33 8. References / 35 9. Annexes / 39 Annex 1: Malaria vectors in the Americas / 39 Annex 2: WHO recommended insecticides for indoor residual spraying / 40 Annex 3: WHO recommended long-lasting insecticide-treated nets / 41 Strategic Orientation Document for Malaria Vector Surveillance and Control in Latin America and the Caribbean 3 About this document: This document was produced through the Amazon Malaria Initiative (AMI) and was financed by the U.S. -
New Books Catalogue
Film & Media New Books Catalogue July-December 2020 Stuck in a research rut? A study slump? Learn the skills to get back on course. Sort the method from the madness with Bloomsbury Research Methods and Study Skills – textbooks and guides designed to give students the essential tools they need for their studies. www.bloomsbury.com/researchmethodsandstudyskills 9781350046948 | £21.99 9781474282949 | £23.99 9781441163752 | £22.99 9780826496317 | £22.99 Discover the What Is? Research Methods series of introductions – handy guides to all the main methodologies for researchers. Series Editor: Graham Crow, University of Edinburgh, UK 9781472530073 | £17.99 9781350018273 | £16.99 9781472515407 | £17.99 9781849665957 | £17.99 9781849669030 | £18.99 9781849669733 | £18.99 9781849665247 | £18.99 9781849666060 | £18.99 9781849668170 | £18.99 Discover the full series: www.bloomsbury.com/whatis RM+SS_BertramsBTU_ad.indd 1 24/06/2019 14:06 Contents EBooks BFI Film Classics . 3 ePub and ePdf availability is listed under each book entry. See the Asian and World Cinema ���������������������������������������������������� 5 website for details of vendors, or to puchase individual ebooks direct. Library ebook prices are available from your supplier. European Cinema. 6 Review Copies British Cinema �������������������������������������������������������������������� 8 Email [email protected] (Americas) Hollywood Cinema. 9 / [email protected] (UK / Rest of World). Film Theory. 9 Standing Orders Film History. 11 Many series are available -
Beijing Hits Back After US Treasury Labels China a Currency Manipulator
BUSINESS WITH PERSONALITY HEARTBREAK BLAST FROM THE PAST HOTEL WHEN A NEW SUPERCHARGED HOLIDAYS GO £90K CLASSIC MINI P18 WRONG P17 WEDNESDAY 7 AUGUST 2019 ISSUE 3,430 CITYAM.COM FREE CRYIN’ KING Disney investors GOVE POINTS tearful despite box office success THE FINGER CATHERINEAT NEILAN BRUSSELSthe backing of the Commons. ratification of the withdrawal agree- “We can’t have a deal that doesn’t ment, or further extension or revoca- @CatNeilan command the confidence of the gov- tion of Article 50... [I am] certainly not THE UK has accused the EU of refusing ernment, the parliament and the fatalistic about that. to budge on Brexit negotiations and in- country and that is why we have been “Our position is that the withdrawal sisted the bloc “must change its clear with the European Union that agreement, including the backstop is stance” if a no-deal exit is to be dodged. we need a new approach,” he closed, but there is always room for Ireland’s Leo Varadkar yesterday responded. talks and negotiations. We have said said he was confident a no-deal Brexit “We stand ready to engage with the we can certainly make changes to the could still be avoided, despite EU to negotiate in good faith… political declaration, and we have Prime Minister Boris Johnson We will put all our energy demonstrated before to offer clarifica- stressing the UK will leave into making sure we can tions as we did at the request of Prime the European Union “do secure that good deal. Minister May in the past.” or die”, come 31 Octo- “At the moment it’s Yesterday the pound hit its lowest ber. -
Introduction to Ascential Our Investment Case
INTRODUCTION TO ASCENTIAL OUR INVESTMENT CASE Clear long-term vision. Helping leading global brands connect with their customers in a data-driven world. Structural growth. Demand for information, data & analytics driven by growth of digital commerce. Market leaders. We are leaders, with a unique blend of specialisms, in the high growth areas in which we operate. Robust business model. High recurring and repeat revenue, with more than 50% revenues from digital subscription and platforms, across diverse global customer base. Attractive financial profile. Track record of high single digit revenue growth, strong margins and cash generation, supported by sound capital allocation. Introduction to Ascential 2 OUR CUSTOMER PROPOSITION Our information products and platforms support our customers to do three simple things… CREATE THE RIGHT MAXIMISE THE OPTIMISE DIGITAL PRODUCTS BRAND MARKETING COMMERCE IMPACT Know which products Know how to get Know how to execute the consumer wants maximum creativity with with excellence on the tomorrow. optimised media. winning platforms. 1. 2. 3. Introduction to Ascential 3 SEGMENTAL OVERVIEW –2019 Segment Revenue % Revenue1 Growth1 EBITDA2 Margin Business Model Advisory 10% Digital Subscriptions Product £86m 21% +8% £36m 42% & Platforms 90% Design Digital Subscriptions Advisory & Platforms 11% Marketing £136m 32% +9% £51m 37% 37% Events 52% Advisory 6% Digital Subscriptions & Sales - Platforms 94% Digital £90m 22% +21% £13m 15% Commerce Sales - Digital Subscriptions & Events Platforms 4% Non Digital £68m 16% -
VOLUME II Public School Code of 1949 Goods and Services Expenditures Fiscal Year 2017-2018
Public School Code of 1949 Goods and Services Expenditures Fiscal Year 2017-2018 VOLUME II Temple University Financial Disclosure Report Purchase of Goods and Services Contracts Notes and Definitions The following report provides the required disclosures for reporting the purchase of goods and services contracts. The University’s Banner Finance System does not include data enabling the distinction between the purchases of goods and services. Therefore, a single report is provided that includes both. Expenditures are categorized in the attached report using the following categories: General Supplies & Services: o General supplies, expendable equipment and software. Health Service Programs: o Animal lab, professional billing and other outside professional services. Insurance: o Malpractice, property, general liability, and employees insurances. Interest & Taxes: o Bond interest, real estate tax and debt service costs. Library: o Books, electronic periodicals, subscriptions and film. Professional Fees & Contracts: o Auditing, legal and collection fees and subcontracts. Property, Plant & Equipment: o Capital equipment, buildings and building improvements. Rent: o Equipment, building and office rentals. Repairs & Maintenance: o Equipment repair, maintenance of buildings and grounds. Telecommunications: o Telephone equipment, data communications and cellular services. Travel: o Travel agency fees, foreign and domestic travel expenses. Utilities: o Electric, gas, water, sewer, steam, chilled water and other miscellaneous utilities expenses. Each entry provides the category into which the purchase falls, the vendor name and address and the amount of the purchase. There is no more than one entry per vendor for a single category within a responsibility center. Purchases of goods and services in the Disclosure Report include those which equal or exceed $1,000 for each vendor from all Budgeted Operating Funds including Temple University Physicians. -
Pioneering Tool to Manage Media Industry's Digital Carbon Footprint 13 January 2020
Pioneering tool to manage media industry's digital carbon footprint 13 January 2020 industry understand and manage the carbon impact of digital media. Mapping the carbon footprint of digital services like advertising, publishing and broadcasting is difficult because the underlying technological systems are hugely complex and constantly shifting. Media content passes through content delivery networks, data centres, web infrastructure and user devices, to name just a few, with each element of the delivery chain having different owners. With climate change high on the agenda, DIMPACT The online tool with help media industry manage its will allow participating companies to understand digital carbon footprint. Credit: Pixabay/ University of their 'downstream' carbon impacts, right through to Bristol the end-user. This, in turn, will enable more informed decision-making to reduce the overall carbon footprint of digital services. A collaboration between computer scientists at the University of Bristol and nine major media "We know that more and more of our interactions companies, including ITV and BBC, will help the happen online, and screens play an ever more media industry understand and manage the important role in our lives. We can say with significant carbon impacts of digital content. absolute certainty that the digital economy will continue to grow. What we don't know is how those The 12-month collaboration, facilitated by modes of digital consumption translate into carbon sustainability experts, Carnstone, will see impacts and where the 'hotspots' reside. DIMPACT University of Bristol researchers working with will change that," said Christian Toennesen, Senior sustainability and technology teams at the BBC, Partner at Carnstone and DIMPACT's initiator and Dentsu Aegis Network, Informa, ITV, Pearson, product manager. -
New Books Catalogue
Classical Studies & Archaeology New Books Catalogue July-December 2020 Stuck in a research rut? A study slump? Learn the skills to get back on course. Sort the method from the madness with Bloomsbury Research Methods and Study Skills – textbooks and guides designed to give students the essential tools they need for their studies. www.bloomsbury.com/researchmethodsandstudyskills 9781350046948 | £21.99 9781474282949 | £23.99 9781441163752 | £22.99 9780826496317 | £22.99 Discover the What Is? Research Methods series of introductions – handy guides to all the main methodologies for researchers. Series Editor: Graham Crow, University of Edinburgh, UK 9781472530073 | £17.99 9781350018273 | £16.99 9781472515407 | £17.99 9781849665957 | £17.99 9781849669030 | £18.99 9781849669733 | £18.99 9781849665247 | £18.99 9781849666060 | £18.99 9781849668170 | £18.99 Discover the full series: www.bloomsbury.com/whatis RM+SS_BertramsBTU_ad.indd 1 24/06/2019 14:06 EBooks Contents ePub and ePdf availability is listed under each book entry. See the website for details of vendors, or to puchase individual ebooks direct. Library ebook prices are available from your supplier. Archaeology and Egyptology. .2 Ancient Greece. .3 Review Copies Email [email protected] (Americas) Ancient Rome / Late Antiquity . .4 / [email protected] (UK / Rest of World). Ancient Greek and Latin Literature. .5 Standing Orders Ancient Drama ����������������������������������������������������������������6 Many series are available on standing order. Ancient Philosophy. .7 Please contact our trade ordering departments (see pages 10 and 11). Classical Reception. .8 Representatives, Agents & Distributors ������������������������10 Translation Rights Available unless otherwise indicated. Key to Symbols Available on inspection / as exam copies: order online at www.bloomsbury.com. To request any other PB or eBook, email [email protected] (Americas) / [email protected] (UK / Rest of World).