Qty: 100 µg/200 µl Mouse anti-Neurofilament-200 kD (NF-H)

Catalog No. 13-1000 Lot No. See product label

Mouse anti-Neurofilament-200 kD (NF-H)

FORM Liquid. Purified (from mouse ascites fluid) in PBS containing 0.1% sodium azide (NaN 3) at a concentration of 0.5 mg/ml.

(1) CLONE: RMO-24 ISOTYPE: IgG 1, kappa

CLONING PARTNER: Sp/2

IMMUNOGEN: Rat neurofilaments

SPECIFICITY This antibody reacts with the 200 kD polypeptides of human neurofilament. It specifically recognizes a phosphate-dependent epitope in the tail domain of NF-H. Dephosphorylation of the NF-H will diminish the immunoreactivity of RMO-24.

APPLICATION Neurofilament (NFPs) are a macromolecular complex comprised of 3 polypeptides designated as NF-L, NF-M and NF-H. NFPs are found in the perikarya, particularly in neuronal throughout the central and peripheral . Since NFPs are major structural proteins and biochemically quite stable, to NFPs are useful probes in studies of neuronal expression, morphology, connectivity and pathology. The presence or absence of NFP in a variety of nervous system or neuroendocrine tumors can provide useful information about the original type of the tumor. Alterations in the NFP expression pattern are also seen in most toxin-induced, sporadic and heriditary axonopathies ocurring in humans and animals.

USAGE

Immunohistochemistry* (1,2,3) : 5-10 µg/ml Immunoblotting (1,2) : 0.5-1.0 µg/ml Immunoprecipitation (2) : 2-5 µg ELISA: 0.1-0.5 µg/ml

*This antibody is suitable for immunohistochemical of Bouin's and alcohol-fixed paraffin-embedded or frozen tissue sections. But formalin-fixed paraffin-embedded tissue section can also be used if it is pretreated with trypsin digestion (Cat No. 00-3008). To stain, incubate 30-60 minutes at room temperature or overnight at 4°C. Specificity is confirmed by a Western Blot using rat brain lysate.

STORAGE Store at 2-8°C for up to one month. Store at -20°C for long term storage. Do not repeated freeze and thaw.

BACKGROUND Neurofilaments are intermediate (10-12nm) filaments located specifically in . There are three classes of neurofilaments: NF-L (68 kDa), NF-M (160 kDa). And NF-H (200 kDa). The neurofilaments are long helical proteins which polymerize to form a rigid in the . This polymerized network is composed of all three filaments, and the stoichiometry of association varies during development. Neurofilaments are posttranslationally modified both by and . Like other proteins, phosphorylation likely mediates neurofilament function remains unclear.

PI131000 MAN0008040 Rev 1.00 Effective Date: 16 MAY 2013

For Research Use Only. Not for use in diagnostic procedures. Manufacturing site: 7335 Executive Way | Frederick, MD 21704 | Toll Free in USA 800.955.6288 www.lifetechnologies.com

REFERENCES 1. Lee VM-Y, et al; J Neurosci 7:3474-3488, 1987 2. Clark and Lee; J Neurosci Res 30:116-123 (1991) 3. Carden MJ et al; J Neurosci 7:3489-3504 (1987) 4. Trojanowski, JQ; et al.: Brain Pathol 3:45-54 (1993) 5. Trojanowski et al; Molec Chem Neuropath 17:121-135 (1992) 6. Schmidt et al; Lab Invest 56:282-294 (1987) 7. Schmidt ML et al; Am J Pathol 136:1069 (1990) 8. Schmidt ML et al; Am J Pathol 139:53 (1991)

RELATED PRODUCTS

Product Clone Cat. No. Ms x Neurofilament-68 kD (NF-L) DA2 13-0400 Ms Ms x Neurofilament-160 kD (NF-M) RMO-270 13-0700 Ms x Neurofilament-160/200 kD (NF-M+H) RmdO-20 13-1300 Ms x Neurofilament-L+M+H (PAN) RMO-24 18-0171 Ms x Neurofilament-160 kD (NF-M) RMO-44 13-0500

Protein A Sepharose ® 4B 10-1041 rec- G Sepharose ® 4B 10-1241

Sepharose ® is a registered trademark of Pharmacia LKB.

Explanation of symbols Symbol Description Symbol Description

Manufacturer Catalog number

Use by Temperature limitation

Consult instructions for use Caution, consult accompanying documents

Batch code

PI131000 MAN0008040 Rev 1.00 Effective Date: 16 MAY 2013

For Research Use Only. Not for use in diagnostic procedures. Manufacturing site: 7335 Executive Way | Frederick, MD 21704 | Toll Free in USA 800.955.6288 www.lifetechnologies.com

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