Mycobacterium Avium Complex Olecranon Bursitis Resolves
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Discovery of a Novel Mycobacterium Asiaticum PRA-Hsp65 Pattern
Infection, Genetics and Evolution 76 (2019) 104040 Contents lists available at ScienceDirect Infection, Genetics and Evolution journal homepage: www.elsevier.com/locate/meegid Short communication Discovery of a novel Mycobacterium asiaticum PRA-hsp65 pattern T ⁎ William Marco Vicente da Silva , Mayara Henrique Duarte, Luciana Distásio de Carvalho, Paulo Cesar de Souza Caldas, Carlos Eduardo Dias Campos, Paulo Redner, Jesus Pais Ramos National Reference Laboratory for Tuberculosis, Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública, Fiocruz, RJ, Brazil ARTICLE INFO ABSTRACT Keywords: Twenty-one pulmonary sputum samples from nine Brazilian patients were analyzed by the PRA-hsp65 method PRA-hsp65 for identification of Mycobacterium species and the results were compared by sequencing. We reported a mu- Identification tation at the position 381, that generates a suppression cutting site in the BstEII enzyme, thus leading to a new M. asiaticum PRA-hsp65 pattern for M. asiaticum identification. Nontuberculous mycobacteria (NTM) are opportunistic human pa- widely used for identification of Mycobacterium species. The PRA-hsp65 thogens. NTM are widespread in nature and are found in environmental methodology consists of restriction analysis of a 441 bp PCR fragment sources, including water, soil, and aerosols. They are resistant to most of the hsp65 gene with enzymes BstEII and HaeIII (Campos et al., 2012; disinfectants, including those used in treated water. More than 170 Devulder, 2005; Tamura et al., 2011; Verma et al., 2017). Nowadays, NTM species have been described (http://www.bacterio.net/ M. asiaticum has a single pattern described as type 1 in the PRAsite mycobacterium.html), however, the knowledge about NTM infections database (http://app.chuv.ch/prasite/index.html) based on the fol- is still limited (Chin'ombe et al., 2016; Tortoli, 2014). -
Mycobacterium Arupense Among the Isolates of Non-Tuberculous Mycobacteria from Human, Animal and Environmental Samples
Veterinarni Medicina, 55, 2010 (8): 369–376 Original Paper Mycobacterium arupense among the isolates of non-tuberculous mycobacteria from human, animal and environmental samples M. Slany1, J. Svobodova2, A. Ettlova3, I. Slana1, V. Mrlik1, I. Pavlik1 1Veterinary Research Institute, Brno, Czech Republic 2Regional Institute of Public Health, Brno, Czech Republic 3BioPlus, s.r.o., Brno, Czech Republic ABSTRACT: Mycobacterium arupense is a non-tuberculous, potentially pathogenic species rarely isolated from humans. The aim of the study was to ascertain the spectrum of non-tuberculous mycobacteria within 271 sequenced mycobacterial isolates not belonging to M. tuberculosis and M. avium complexes. Isolates were collected between 2004 and 2009 in the Czech Republic and were examined within the framework of ecological studies carried out in animal populations infected with mycobacteria. A total of thirty-three mycobacterial species were identified. This report describes the isolation of M. arupense from the sputum of three human patients and seven different animal and environmental samples collected in the last six years in the Czech Republic: one isolate from leftover refrigerated organic dog food, two isolates from urine and clay collected from an okapi (Okapia johnstoni) and antelope bongo (Tragelaphus eurycerus) enclosure in a zoological garden, one isolate from the soil in an eagle’s nest (Haliaeetus albicilla) band two isolates from two common vole (Microtus arvalis) livers from one cattle farm. All isolates were identified by biochemical tests, morphology and 16S rDNA sequencing. Also, retrospective screening for M. arupense occurrence within the collected isolates is presented. Keywords: 16S rDNA sequencing; non-tuberculous mycobacteria; ecology Non-tuberculous mycobacteria (NTM) are ubiq- According to the commonly used Runyon clas- uitous in the environment and are responsible for sification scheme, NTM are categorized by growth several diseases in humans and/or animals known rate and pigmentation. -
Epidemiology of Infection by Nontuberculous Mycobacteria JOSEPH O
CLINICAL MICROBIOLOGY REVIEWS, Apr. 1996, p. 177–215 Vol. 9, No. 2 0893-8512/96/$04.0010 Copyright q 1996, American Society for Microbiology Epidemiology of Infection by Nontuberculous Mycobacteria JOSEPH O. FALKINHAM III* Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061-0406 INTRODUCTION .......................................................................................................................................................178 The Nontuberculous Mycobacteria.......................................................................................................................178 Nontuberculous Mycobacterial Disease before the AIDS Epidemic................................................................179 Nontuberculous Mycobacterial Disease after the AIDS Epidemic ..................................................................179 Current Trends in the Epidemiology of Nontuberculous Mycobacterial Disease .........................................179 Chemotherapy of Nontuberculous Mycobacterial Infections............................................................................180 Detection, Recovery, and Identification of Nontuberculous Mycobacteria.....................................................181 Ecology and Physiology of Nontuberculous Mycobacteria................................................................................181 Virulence of Nontuberculous Mycobacteria ........................................................................................................182 -
Sneha Bowalekar and Rahul Gadkari.Pdf
Int.J.Curr.Microbiol.App.Sci (2021) 10(02): 476-482 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 10 Number 02 (2021) Journal homepage: http://www.ijcmas.com Case Study https://doi.org/10.20546/ijcmas.2021.1002.056 A Rare Case of Olecranon Bursitis caused by Mycobacterium abscessus Treated with Surgical Intervention Sneha Bowalekar* and Rahul Gadkari 1Consultant Microbiologist, Dr. Jariwala Laboratory, 1st floor, Rasraj Heights, Rokadia Lane, Near Gokul Hotel, Boriwali West, Mumbai, Maharashtra, India 2Consultant Orthopedic Surgeon, Sailee Hospital and Diagnostic Centre, Prathamesh Horizon, New Link Road, Boriwali West, Mumbai, Maharashtra, India *Corresponding author ABSTRACT K e yw or ds The present case of Mycobacterium abscessus olecranon bursitis in an M. abscessus , immunocompetant male treated with surgical drainage without requirement of Olecranon bursitis, antimycobacterial therapy. Nontuberculous mycobacteria are widely listed as Immunocompetant an infective cause of olecranon bursitis and other skin and soft tissue Article Info infections. Direct inoculation of the organism during trauma leads to musculoskeletal infections in particular. We could not find any other case Accepted: report of olecranon bursitis caused by M. abscessus in the literature. This case 07 January 2021 extends the view about nontuberculous mycobacterial infections in Available Online: 10 February 2021 immunocometant adults with co-morbid condition such as diabetes. Introduction olecranon bursitis caused by NTM identified as Mycobacterium abscessus. The infection Nontuberculous mycobacteria (NTM) are was treated with surgical drainage of increasingly being reported as cause of olecranon bursa fluid. Clinical follow up of infections in immunocompromised as well as patient was uneventful. immunocompetant patients. -
The Crystal Structure of Rv2991 from Mycobacterium Tuberculosis : an F 420 Binding Protein with Unknown Function
This is a repository copy of The crystal structure of Rv2991 from Mycobacterium tuberculosis : An F 420 binding protein with unknown function. White Rose Research Online URL for this paper: https://eprints.whiterose.ac.uk/147627/ Version: Accepted Version Article: Benini, Stefano, Haouz, Ahmed, Proux, Florence et al. (2 more authors) (2019) The crystal structure of Rv2991 from Mycobacterium tuberculosis : An F 420 binding protein with unknown function. JOURNAL OF STRUCTURAL BIOLOGY. pp. 216-224. ISSN 1047-8477 https://doi.org/10.1016/j.jsb.2019.03.006 Reuse This article is distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs (CC BY-NC-ND) licence. This licence only allows you to download this work and share it with others as long as you credit the authors, but you can’t change the article in any way or use it commercially. More information and the full terms of the licence here: https://creativecommons.org/licenses/ Takedown If you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing [email protected] including the URL of the record and the reason for the withdrawal request. [email protected] https://eprints.whiterose.ac.uk/ The crystal structure of Rv2991 from Mycobacterium tuberculosis: An F420 binding protein with unknown function Stefano Beninia, ⁎ [email protected] Ahmed Haouzb Florence Prouxb, 1 Pedro Alzaric Keith Wilsond a Bioorganic Chemistry and Bio-Crystallography Laboratory (B2Cl), Faculty of Science and Technology, Free University of Bolzano, Piazza Università 5, Bolzano 39100, Italy bC2RT-Plateforme de cristallographie, Institut Pasteur, CNRS UMR 3528, 75724 Paris Cedex 15, France cUnité de Microbiologie Structurale, Institut Pasteur, CNRS UMR 3528, Université Paris Diderot, Sorbonne Paris Cité, 75724 Paris Cedex 15, France d York Structural Biology Laboratory, Department of Chemistry, University of York, Heslington, York YO10 5DD, UK ⁎Corresponding author. -
Clinical and Epidemiological Features
P0506 Paper Poster Session III Nontuberculous mycobacteria Nontuberculous mycobacteria in a third level hospital in Spain: clinical and epidemiological features G. Barbeito Castiñeiras1, M. Otero1, L. Ferreiro1, R. Trastoy1, J.J. Costa1, V. Tuñez1, M.L. Pérez del Molino1 1Clinical Microbiology Department- Complexo Hospitalario Universitario de Santiago de Compostela, Santiago de Compostela, Spain INTRODUCTION In the last few years, we have been attending to an increasing number of isolations of non-tuberculous mycobacteria (NTM) in the health area of Santiago de Compostela (458.759 inhabitants). Our objective is to study the epidemiology of those infections caused by NTM, their associated factors and their clinical significance. METHOD Retrospective study of NTM isolations carried out from 2005 to 2013. Data sources: Microbiology Information System (OpenLab) and the electronic clinical history of Galicia (IANUS). Statistical analysis: SPSSv.20. Microbiological techniques: auramine staining, and the growth in liquid media (MGIT, Bactec 960, Becton Dickinson) 45 days and solid culture of Coletsos ® 8 weeks. Identification:phenotypic and genotypic methods: GenoType®Mycobacterium CM/AS (Hain Lifescience). For diagnosis, the criteria from the American Thoracic Society / Infectious Diseases Society of America (ATS/IDSA) 2007 were applied and the revision of the clinical history was used for the evaluation of clinical significance. RESULTS During those 9 years of study, a total of 456 strains were aisolated (Mycobacterium avium complex 34,65%, Mycobacterium intracellulare 20,83%, Mycobacterium xenopi 11,84%, Mycobacterium abscessus 9,21%, others 23,47%), concerning 212 patients. 91 patients fulfilled the NTM disease criteria of the ATS/IDSA (19,96%). The average age was 61 (range 1-89), 61,54% were male. -
Nontuberculous Mycobacteria in Respiratory Samples from Patients with Pulmonary Tuberculosis in the State of Rondônia, Brazil
Mem Inst Oswaldo Cruz, Rio de Janeiro, Vol. 108(4): 457-462, June 2013 457 Nontuberculous mycobacteria in respiratory samples from patients with pulmonary tuberculosis in the state of Rondônia, Brazil Cleoni Alves Mendes de Lima1,2/+, Harrison Magdinier Gomes3, Maraníbia Aparecida Cardoso Oelemann3, Jesus Pais Ramos4, Paulo Cezar Caldas4, Carlos Eduardo Dias Campos4, Márcia Aparecida da Silva Pereira3, Fátima Fandinho Onofre Montes4, Maria do Socorro Calixto de Oliveira1, Philip Noel Suffys3, Maria Manuela da Fonseca Moura1 1Centro Interdepartamental de Biologia Experimental e Biotecnologia, Universidade Federal de Rondônia, Porto Velho, RO, Brasil 2Laboratório Central de Saúde Pública de Rondônia, Porto Velho, RO, Brasil 3Laboratório de Biologia Molecular Aplicada a Micobactérias, Instituto Oswaldo Cruz 4Centro de Referência Professor Hélio Fraga, Escola Nacional de Saúde Pública-Fiocruz, Rio de Janeiro, RJ, Brasil The main cause of pulmonary tuberculosis (TB) is infection with Mycobacterium tuberculosis (MTB). We aimed to evaluate the contribution of nontuberculous mycobacteria (NTM) to pulmonary disease in patients from the state of Rondônia using respiratory samples and epidemiological data from TB cases. Mycobacterium isolates were identified using a combination of conventional tests, polymerase chain reaction-based restriction enzyme analysis of hsp65 gene and hsp65 gene sequencing. Among the 1,812 cases suspected of having pulmonary TB, 444 yielded bacterial cultures, including 369 cases positive for MTB and 75 cases positive for NTM. Within the latter group, 14 species were identified as Mycobacterium abscessus, Mycobacterium avium, Mycobacterium fortuitum, Myco- bacterium intracellulare, Mycobacterium gilvum, Mycobacterium gordonae, Mycobacterium asiaticum, Mycobac- terium tusciae, Mycobacterium porcinum, Mycobacterium novocastrense, Mycobacterium simiae, Mycobacterium szulgai, Mycobacterium phlei and Mycobacterium holsaticum and 13 isolates could not be identified at the species level. -
Rapid Detection and Differentiation of Mycobacterial Species Using a Multiplex PCR System
Revista da Sociedade Brasileira de Medicina Tropical 46(4):447-452, Jul-Aug, 2013 http://dx.doi.org/10.1590/0037-8682-0097-2013 MCaseajor ReportArticle Rapid detection and differentiation of mycobacterial species using a multiplex PCR system Andrea Santos Lima[1], Rafael Silva Duarte[2], Lílian Maria Lapa Montenegro[1] and Haiana Charifker Schindler[1] [1]. Departamento de Imunologia, Centro de Pesquisas Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, PE. [2]. Instituto de Microbiologia, Universidade Federal do Rio Janeiro, Rio de Janeiro, RJ. ABSTRACT Introduction: The early diagnosis of mycobacterial infections is a critical step for initiating treatment and curing the patient. Molecular analytical methods have led to considerable improvements in the speed and accuracy of mycobacteria detection. Methods: The purpose of this study was to evaluate a multiplex polymerase chain reaction system using mycobacterial strains as an auxiliary tool in the differential diagnosis of tuberculosis and diseases caused by nontuberculous mycobacteria (NTM) Results: Forty mycobacterial strains isolated from pulmonary and extrapulmonary origin specimens from 37 patients diagnosed with tuberculosis were processed. Using phenotypic and biochemical characteristics of the 40 mycobacteria isolated in LJ medium, 57.5% (n=23) were characterized as the Mycobacterium tuberculosis complex (MTBC) and 20% (n=8) as nontuberculous mycobacteria (NTM), with 22.5% (n=9) of the results being inconclusive. When the results of the phenotypic and biochemical tests in 30 strains of mycobacteria were compared with the results of the multiplex PCR, there was 100% concordance in the identifi cation of the MTBC and NTM species, respectively. A total of 32.5% (n=13) of the samples in multiplex PCR exhibited a molecular pattern consistent with NTM, thus disagreeing with the fi nal diagnosis from the attending physician. -
Mixed Cutaneous Infection Caused by Mycobacterium Szulgai and Mycobacterium Intermedium in a Healthy Adult Female: a Rare Case Report
Hindawi Publishing Corporation Case Reports in Dermatological Medicine Volume 2015, Article ID 607519, 4 pages http://dx.doi.org/10.1155/2015/607519 Case Report Mixed Cutaneous Infection Caused by Mycobacterium szulgai and Mycobacterium intermedium in a Healthy Adult Female: A Rare Case Report Amresh Kumar Singh,1 Rungmei S. K. Marak,2 Anand Kumar Maurya,3 Manaswini Das,2 Vijaya Lakshmi Nag,3 and Tapan N. Dhole2 1 Department of Microbiology, BRD Medical College, Gorakhpur, Uttar Pradesh 273013, India 2Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow 226014, India 3Department of Microbiology, All India Institute of Medical Sciences, Jodhpur 342005, India Correspondence should be addressed to Amresh Kumar Singh; [email protected] Received 2 December 2014; Revised 2 February 2015; Accepted 2 February 2015 Academic Editor: Kowichi Jimbow Copyright © 2015 Amresh Kumar Singh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Nontuberculous mycobacteria (NTMs) are ubiquitous and are being increasingly reported as human opportunistic infection. Cutaneous infection caused by mixed NTM is extremely rare. We encountered the case of a 46-year-old female, who presented with multiple discharging sinuses over the lower anterior abdominal wall (over a previous appendectomy scar) for the past 2 years. Microscopy and culture of the pus discharge were done to isolate and identify the etiological agent. Finally, GenoType Mycobacterium CM/AS assay proved it to be a mixed infection caused by Mycobacterium szulgai and M. -
The Impact of Chlorine and Chloramine on the Detection and Quantification of Legionella Pneumophila and Mycobacterium Spp
The impact of chlorine and chloramine on the detection and quantification of Legionella pneumophila and Mycobacterium spp. Maura J. Donohue Ph.D. Office of Research and Development Center of Environmental Response and Emergency Response (CESER): Water Infrastructure Division (WID) Small Systems Webinar January 28, 2020 Disclaimer: The views expressed in this presentation are those of the author and do not necessarily reflect the views or policies of the U.S. Environmental Protection Agency. A Tale of Two Bacterium… Legionellaceae Mycobacteriaceae • Legionella (Genus) • Mycobacterium (Genus) • Gram negative bacteria • Nontuberculous Mycobacterium (NTM) (Gammaproteobacteria) • M. avium-intracellulare complex (MAC) • Flagella rod (2-20 µm) • Slow grower (3 to 10 days) • Gram positive bacteria • Majority of species will grow in free-living • Rod shape(1-10 µm) amoebae • Non-motile, spore-forming, aerobic • Aerobic, L-cysteine and iron salts are required • Rapid to Slow grower (1 week to 8 weeks) for in vitro growth, pH: 6.8 to 7, T: 25 to 43 °C • ~156 species • ~65 species • Some species capable of causing disease • Pathogenic or potentially pathogenic for human 3 NTM from Environmental Microorganism to Opportunistic Opponent Genus 156 Species Disease NTM =Nontuberculous Mycobacteria MAC = M. avium Complex Mycobacterium Mycobacterium duvalii Mycobacterium litorale Mycobacterium pulveris Clinically Relevant Species Mycobacterium abscessus Mycobacterium elephantis Mycobacterium llatzerense. Mycobacterium pyrenivorans, Mycobacterium africanum Mycobacterium europaeum Mycobacterium madagascariense Mycobacterium rhodesiae Mycobacterium agri Mycobacterium fallax Mycobacterium mageritense, Mycobacterium riyadhense Mycobacterium aichiense Mycobacterium farcinogenes Mycobacterium malmoense Mycobacterium rufum M. avium, M. intracellulare, Mycobacterium algericum Mycobacterium flavescens Mycobacterium mantenii Mycobacterium rutilum Mycobacterium alsense Mycobacterium florentinum. Mycobacterium marinum Mycobacterium salmoniphilum ( M. fortuitum, M. -
Association of Non-Tuberculous Mycobacteria with Mycobacterium Leprae in Environment of Leprosy Endemic Regions in India T ⁎ Ravindra P
Infection, Genetics and Evolution 72 (2019) 191–198 Contents lists available at ScienceDirect Infection, Genetics and Evolution journal homepage: www.elsevier.com/locate/meegid Research Paper Association of non-tuberculous mycobacteria with Mycobacterium leprae in environment of leprosy endemic regions in India T ⁎ Ravindra P. Turankara, , Vikram Singha, Hariom Guptaa, Vinay Kumar Pathaka, Madhvi Ahujaa, Itu Singha, Mallika Lavaniaa, Amit K. Dindab, Utpal Senguptaa a The Leprosy Mission Trust India, Stanley Browne Laboratory, TLM community Hospital Nand Nagari, New Delhi 110093, India b Department of Pathology, Institute/University: All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110 029, India ARTICLE INFO ABSTRACT Keywords: Non-tuberculous mycobacteria (NTM) are environmental mycobacteria found ubiquitously in nature. The pre- Mycobacterial species sent study was conducted to find out the presence of various species of NTM in leprosy endemic region along 16S rRNA gene target with Mycobacterium (M) leprae. Water and wet soil samples from the periphery of ponds used by the community Sequencing were collected from districts of Purulia of West Bengal and Champa of Chhattisgarh, India. Samples were pro- FITC cessed and decontaminated followed by culturing on Lowenstein Jensen (LJ) media. Polymerase chain reaction PGL-1 (PCR) was performed using 16S rRNA gene target of mycobacteria and species was confirmed by sequencing M. leprae method. Indirect immune-fluorescent staining of M. leprae from soil was performed using M. leprae-PGL-1 rabbit polyclonal antibody. The phylogenetic tree was constructed by using MEGA-X software. From 380 soil samples 86 NTM were isolated, out of which 34(40%) isolates were rapid growing mycobacteria (RGM) and 52(60%) isolates were slow growing mycobacteria (SGM). -
Nunescosta2016.Pdf
Tuberculosis 96 (2016) 107e119 Contents lists available at ScienceDirect Tuberculosis journal homepage: http://intl.elsevierhealth.com/journals/tube REVIEW The looming tide of nontuberculous mycobacterial infections in Portugal and Brazil Daniela Nunes-Costa a, Susana Alarico a, Margareth Pretti Dalcolmo b, * Margarida Correia-Neves c, d, Nuno Empadinhas a, e, a CNC e Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal b Reference Center Helio Fraga, Fundaçao~ Oswaldo Cruz, FIOCRUZ, MoH, Rio de Janeiro, Brazil c ICVS e Health and Life Sciences Research Institute, University of Minho, Braga, Portugal d ICVS/3B's, PT Government Associate Laboratory, Braga/Guimaraes,~ Portugal e IIIUC e Institute for Interdisciplinary Research, University of Coimbra, Coimbra, Portugal article info summary Article history: Nontuberculous mycobacteria (NTM) are widely disseminated in the environment and an emerging Received 5 May 2015 cause of infectious diseases worldwide. Their remarkable natural resistance to disinfectants and anti- Received in revised form biotics and an ability to survive under low-nutrient conditions allows NTM to colonize and persist in 27 August 2015 man-made environments such as household and hospital water distribution systems. This overlap be- Accepted 16 September 2015 tween human and NTM environments afforded new opportunities for human exposure, and for expression of their often neglected and underestimated pathogenic potential. Some risk factors pre- Keywords: disposing to NTM disease have been