ZW10 Antibody Cat

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ZW10 Antibody Cat. No.: 58-512 ZW10 Antibody Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse This ZW10 antibody is generated from rabbits immunized with a KLH conjugated synthetic IMMUNOGEN: peptide between 59-88 amino acids from the N-terminal region of human ZW10. TESTED APPLICATIONS: WB APPLICATIONS: For WB starting dilution is: 1:1000 PREDICTED MOLECULAR 89 kDa WEIGHT: Properties This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification. CLONALITY: Polyclonal ISOTYPE: Rabbit Ig CONJUGATE: Unconjugated PHYSICAL STATE: Liquid September 26, 2021 1 https://www.prosci-inc.com/zw10-antibody-58-512.html BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide. CONCENTRATION: batch dependent Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: ZW10 ALTERNATE NAMES: Centromere/kinetochore protein zw10 homolog, ZW10 ACCESSION NO.: O43264 GENE ID: 9183 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. The encoded protein binds to centromeres BACKGROUND: during the prophase, metaphase, and early anaphase cell division stages and to kinetochore microtubules during metaphase. REFERENCES: 1) Chan, Y.W., et al. J. Cell Biol. 185(5):859-874(2009) 2) Aoki, T., et al. Mol. Biol. Cell 20(11):2639-2649(2009) 3) Inoue, M., et al. Genes Cells 13(8):905-914(2008) 4) Famulski, J.K., et al. J. Cell Biol. 180(3):507-520(2008) ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 26, 2021 2 https://www.prosci-inc.com/zw10-antibody-58-512.html.

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TANGO1 Builds a Machine for Collagen Export by Recruiting And
RESEARCH ARTICLE TANGO1 builds a machine for collagen export by recruiting and spatially organizing COPII, tethers and membranes Ishier Raote1,2†, Maria Ortega-Bellido1,2†, Anto´ nio JM Santos1,2‡, Ombretta Foresti1,2, Chong Zhang3, Maria F Garcia-Parajo4,5, Felix Campelo4, Vivek Malhotra1,2,5* 1Centre for Genomic Regulation, The Barcelona Institute of Science and Technology, Barcelona, Spain; 2Universitat Pompeu Fabra, Barcelona, Spain; 3SIMBIOsys Group, Department of Information and Communication Technologies, Universitat Pompeu Fabra, Barcelona, Spain; 4ICFO-Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, Castelldefels, Spain; 5Institucio Catalana de Recerca i Estudis Avanc¸ats, Barcelona, Spain Abstract Collagen export from the endoplasmic reticulum (ER) requires TANGO1, COPII coats, and retrograde fusion of ERGIC membranes. How do these components come together to produce a transport carrier commensurate with the bulky cargo collagen? TANGO1 is known to form a ring that corrals COPII coats, and we show here how this ring or fence is assembled. Our data reveal that a TANGO1 ring is organized by its radial interaction with COPII, and lateral interactions with *For correspondence: cTAGE5, TANGO1-short or itself. Of particular interest is the finding that TANGO1 recruits ERGIC [email protected] membranes for collagen export via the NRZ (NBAS/RINT1/ZW10) tether complex. Therefore, †These authors contributed TANGO1 couples retrograde membrane flow to anterograde cargo transport. Without the NRZ equally to this work complex, the TANGO1 ring does not assemble, suggesting its role in nucleating or stabilising this Present address: ‡Division of process. Thus, coordinated capture of COPII coats, cTAGE5, TANGO1-short, and tethers by Hematology, Department of TANGO1 assembles a collagen export machine at the ER.
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Kinetochores, Microtubules, and Spindle Assembly Checkpoint
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Product Name: ZW10 Peptide Polyclonal Antibody, ALEXA FLUOR® 594 Conjugated Catalog No
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32-5283: Recombinant Human ZW10 Interacting Kinetochore Protein
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APOCB2006-04-001 Inhibition of the Mitotic Kinesin Eg5 Induces Mitotic
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