Anthropogenic Litter and Microplastic in Urban Streams: Abundance, Source, and Fate
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Loyola University Chicago Loyola eCommons Master's Theses Theses and Dissertations 2015 Anthropogenic Litter and Microplastic in Urban Streams: Abundance, Source, and Fate Amanda Rae Mccormick Loyola University Chicago Follow this and additional works at: https://ecommons.luc.edu/luc_theses Part of the Ecology and Evolutionary Biology Commons Recommended Citation Mccormick, Amanda Rae, "Anthropogenic Litter and Microplastic in Urban Streams: Abundance, Source, and Fate" (2015). Master's Theses. 3144. https://ecommons.luc.edu/luc_theses/3144 This Thesis is brought to you for free and open access by the Theses and Dissertations at Loyola eCommons. It has been accepted for inclusion in Master's Theses by an authorized administrator of Loyola eCommons. For more information, please contact [email protected]. This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License. Copyright © 2015 Amanda Rae Mccormick LOYOLA UNIVERSITY CHICAGO ANTHROPOGENIC LITTER AND MICROPLASTIC IN URBAN STREAMS: ABUNDANCE, SOURCE, AND FATE A THESIS SUBMITTED TO THE FACULTY OF THE GRADUATE SCHOOL IN CANDIDACY FOR THE DEGREE OF MASTER OF SCIENCE PROGRAM IN BIOLOGY BY AMANDA RAE MCCORMICK CHICAGO, ILLINOIS DECEMBER 2015 Copyright by Amanda Rae McCormick, 2015 All rights reserved. ACKNOWLEDGEMENTS This research was funded by the Illinois Water Resource Center and the Graduate School of Loyola University Chicago. Thank you to my thesis adviser Timothy Hoellein and committee members Martin Berg and John Kelly for their comments on this thesis. Thanks also to Maxwell London, Miguel Rojas, Joshua Hittie, Melaney Dunne, Margaret Sladek, Maxwell Jabay, Joseph Schluep, and the Hoellein Lab at Loyola University Chicago for their assistance in the field and laboratory. Thank you to Roy Kressman, Mike Wisniewski, Bill Perry, Jake Callahan, Jim Gorman, Carl Christensen, and Kyle Sinclair for their assistance in sample collection. Finally, thank you to my family and friends for encouraging me throughout the thesis writing process. iii To Kyle, for your constant support in all past, current, and future endeavors. TABLE OF CONTENTS ACKNOWLEDGEMENTS ............................................................................................... iii LIST OF TABLES ............................................................................................................ vii LIST OF FIGURES ............................................................................................................ x ABSTRACT ..................................................................................................................... xiii CHAPTER I: INTRODUCTION ........................................................................................ 1 Human alterations to global ecosystems ......................................................................... 1 AL distribution and abundance ....................................................................................... 2 Ecological, social, and economic implications of AL ..................................................... 4 Microplastic distribution and sources ............................................................................. 5 Ecological effects of microplastic ................................................................................... 8 Rivers are important in AL and microplastic research .................................................. 11 Thesis objectives ........................................................................................................... 12 CHAPTER II: ABUNDANCE, COMPOSITION, SOURCES, AND MOVEMENT OF ANTHROPOGENIC LITTER IN URBAN STREAMS ......................................... 14 Introduction ................................................................................................................... 14 Materials and Methods .................................................................................................. 16 Study sites .................................................................................................................. 16 Collection and categorization of benthic and riparian AL ........................................ 17 Riverine AL compared to marine benthic habitats and beaches ............................... 19 AL flux: study sites ................................................................................................... 20 AL flux: seasonal measurement ................................................................................ 20 AL flux: biweekly measurement ............................................................................... 22 The effect of sampling interval on AL accumulation rates ....................................... 22 Data analysis .............................................................................................................. 23 Results ........................................................................................................................... 28 AL abundance across streams and between habitats ................................................. 28 Relative AL composition among streams and between habitats ............................... 29 Anthropogenic factors influencing the AL density ................................................... 31 AL density, mass, and composition across ecosystem types ..................................... 32 Seasonal flux: net accumulation and export rates ..................................................... 33 Biweekly flux: net accumulation and export rates .................................................... 34 The effect of sampling interval on AL accumulation rates ....................................... 36 Discussion ..................................................................................................................... 54 AL abundance and composition differed between riparian and benthic habitats ...... 54 v Reach-scale, not watershed-scale factors explained AL abundance and composition........................................................................................................... 54 Hydrology and AL material type influence accumulation and export ...................... 57 AL is a dynamic, mobile substrate ............................................................................ 59 Scaling up AL density, mass, and flux to the watershed ........................................... 60 AL abundance and composition across ecosystems .................................................. 61 AL is abundant and mobile in rivers, with unknown biological effects .................... 64 CHAPTER III: MICROPLASTIC IN URBAN STREAMS: SOURCE, ABUNDANCE, AND SELECTION OF UNIQUE BACTERIA ASSEMBLAGES .... 66 Introduction ................................................................................................................... 66 Materials and Methods .................................................................................................. 69 Study sites .................................................................................................................. 69 Sample collection and microplastic quantification .................................................... 69 DNA extraction and sequencing ................................................................................ 72 Water chemistry......................................................................................................... 73 Data analysis .............................................................................................................. 73 Results ........................................................................................................................... 77 Physical and chemical characteristics of study streams ............................................ 77 Microplastic concentration ........................................................................................ 77 Bacterial assemblages across habitats ....................................................................... 79 Microplastic bacterial assemblages ........................................................................... 82 Discussion ..................................................................................................................... 98 Microplastic concentrations in urban rivers .............................................................. 98 Microplastic concentration in urban rivers is higher than other ecosystems........... 100 Bacterial assemblages colonizing microplastic are unique from natural habitats ... 102 WWTP effluent influences water column bacteria assemblages ............................ 105 The fate of riverine microplastic ............................................................................. 106 Future research and management implications ....................................................... 107 APPENDIX A: SUPPLEMENTAL ANTHROPOGENIC LITTER AND MICROPLASTIC TABLES ........................................................................................ 114 LITERATURE CITED ................................................................................................... 120 VITA................................................................................................................................138 vi LIST OF TABLES Table 1. Location and land use characteristics for the 15 study reaches in streams used for anthropogenic litter (AL) characterization…………………………………...25 Table 2. Anthropogenic activity for the 15 study reaches in