Luteinizing Hormone (LH)

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Clinical Immunology Laboratory TEST: Luteinizing Hormone (LH) PRINCIPLE: LH is a dimeric glycoprotein hormone secreted by the anterior pituitary in response to hypothalamic gonadotrophin releasing hormone. The -subunit is common to other glycoprotein hormones, while the -subunit, which confers biological activity, has some homology with that of human chorionic gonadotrophin. During the menstrual cycle, follicle stimulating hormone (FSH) stimulates growth of the ovarian follicle which, when mature, ovulates in response to a surge of LH and, to a lesser extent, of FSH. Ovarian steroids are the primary negative feedback control for LH secretion. At menopause, reduced ovarian negative feedback results in elevated LH concentrations. LH concentrations also tend to be elevated in women of pre-menopausal age who experience ovarian failure, or whose ovaries failed to mature during puberty. SPECIMEN REQUIREMENTS: 2 ml serum from blood collected in red top tube without additive or in a serum separator tube with gel barrier. Separate the serum from the clot to avoid hemolysis: red top tube – transfer serum into plastic transport vial, gel tube – spin. Transport to the lab at room temperature. Store at room temperature for up to 8h, refrigerate for up to 48h. Store frozen at -20°C or below for up to 30 days. Avoid repeated freeze-thaw cycles. METHOD: Enhanced Chemiluminescence. REFERENCES: 1. Carlsen RB et al. Human chorionic gonadotrophin. Linear amino acid sequence of the beta subunit. J Biol Chem. 248: 6810-6827; 1973. 2. Short RV. The control of menstruation. Br J Hosp Med. 7: 552-555; 1972. 3. Hillier SG. Current Concepts of the Roles of Follicle-Stimulating and Luteinizing Hormone in Folliculogenesis. Human Reproduction. 9:188-191; 1994. Normal Range: Normal Female Mid Follicular Phase: 2.12-10.89 mIU/ml Normal Female Mid Cycle Peak: 19.18-103.03 mIU/ml Normal Mid Luteal Phase: 1.20-12.86 mIU/ml Postmenopausal Female: 10.87-58.64 mIU/ml Normal Male: 1.24-8.62 mIU/ml Turnaround Time: 3 days 75.

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Human Chorionic Gonadotropin (HCG), a Polypeptide Hormone Produced by the Human
45792G/Revised: April 2011 CHORIONIC GONADOTROPIN FOR INJECTION, USP DESCRIPTION: Human chorionic gonadotropin (HCG), a polypeptide hormone produced by the human placenta, is composed of an alpha and a beta sub-unit. The alpha sub-unit is essentially identical to the alpha sub-units of the human pituitary gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone (FSH), as well as to the alpha sub-unit of human thyroid-stimulating hormone (TSH). The beta sub-units of these hormones differ in amino acid sequence. Chorionic gonadotropin is obtained from the human pregnancy urine. It is standardized by a biological assay procedure. Chorionic Gonadotropin for Injection, USP is available in multiple dose vials containing 10,000 USP Units with accompanying Bacteriostatic Water for Injection for reconstitution. When reconstituted with 10 mL of the accompanying diluent each vial contains: Chorionic gonadotropin 10,000 Units Mannitol 100 mg Benzyl alcohol 0.9% Water for Injection q.s. Buffered with dibasic sodium phosphate and monobasic sodium phosphate. Hydrochloric acid and/or sodium hydroxide may have been used for pH adjustment (6.0 Reference ID: 2933198 8.0). Nitrogen gas is used in the freeze drying process. CLINICAL PHARMACOLOGY: The action of HCG is virtually identical to that of pituitary LH, although HCG appears to have a small degree of FSH activity as well. It stimulates production of gonadal steroid hormones by stimulating the interstitial cells (Leydig cells) of the testis to produce androgens and the corpus luteum of the ovary to produce progesterone. Androgen stimulation in the male leads to the development of secondary sex characteristics and may stimulate testicular descent when no anatomical impediment to descent is present.
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Gonadotropin and Testosterone Measurements After
Pediat. Res. 10: 46-51 (1976) Estradiol puberty estrogen sexual differentiation gonadotropins testosterone maturation Gonadotropin and Testosterone Measurements after Estrogen Administration to Adult Men, Prepubertal and Pubertal Boys, and Men with Hypogonadotropism: Evidence for Maturation of Positive Feedback in the Male HOWARD E. KULIN"" AND EDWARD 0. REITER Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, USA Extract MATERIALS AND METHODS Nineteen male subjects were given five daily injections of SUBJECTS 17~-estradiol and circulating levels of estradiol (E 2 ), testosterone (T), and gonadotropins were determined by radioimmunoassay Seven normal, adult men (ages 20-22) and one male (age 21) before, during, and after the steroid course. Peak levels of E 2 with the syndrome of vanishing testes (I) were hospitalized for attained during the 5 days of treatment ranged from 173-577 pg/ml. study at the Clinical Center of the National Institutes of Health. Four of seven normal adult men and one castrate man demonstrated Each patient was given five daily intramuscular injections of IO or suppression of follicle-stimulating hormone ( FSH) and luteinizing 15 ,ug/kg body weight of 17~-estradiol (E2 ) (20) and blood samples hormone ( LH) with a subsequent rise in LH ( positive feedback) were obtained every 12-24 hr before, during, and after the estrogen while E 2 levels remained elevated. A rise in T was associated with course (see Table I). the LH increment in the four normal men. Nine pre-, early, or Nine endocrinologically normal boys between the ages of 7 and midpubertal boys and two men with hypogonadotropic hypogo 18 were studied in the course of evaluation for short stature, nadism displayed only gonadotropin suppression after E 2 adminis precocious puberty, or delayed adolescence.
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Basal Serum Luteinizing Hormone Value As the Screening Biomarker in Female Central Precocious Puberty
Original article https://doi.org/10.6065/apem.2019.24.3.164 Ann Pediatr Endocrinol Metab 2019;24:164-171 Basal serum luteinizing hormone value as the screening biomarker in female central precocious puberty Seung Heo, MD1, Purpose: Precocious puberty refers to the development of secondary sex Young Seok Lee, MD, PhD2, characteristics before ages 8 and 9 years in girls and boys, respectively. Central Jeesuk Yu, MD, PhD1 precocious puberty (CPP) is caused by premature activation of the hypothalamus- pituitary-gonadal (HPG) axis and causes thelarche in girls before the age of 8. A 1Department of Pediatrics, Dankook gonadotropin-releasing hormone (GnRH) stimulation test is the standard diagnostic University Hospital, Dankook University modality for diagnosing CPP. However, the test cannot always be used for screening College of Medicine, Cheonan, Korea because it is expensive and time-consuming. This study aimed to find alternative 2 Department of Diagnostic Radiology, reliable screening parameters to identify HPG axis activation in girls <8 years old Dankook University Hospital, Dankook (CPP) and for girls 8–9 years old (early puberty, EP). University College of Medicine, Methods: From January 2013 to June 2015, medical records from 196 girls younger Cheonan, Korea than 9 years old with onset of breast development were reviewed, including 126 girls who had a bone age (BA) 1 year above their chronological age. All patients underwent a GnRH stimulation test, and 117 underwent pelvic sonography. The girls were divided into 4 groups based on age and whether the GnRH stimulation test showed evidence of central puberty. Subanalyses were also conducted within each group based on peak luteinizing hormone (LH) level quartiles.
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Pro-MCH (1D1): Sc-293231
SANTA CRUZ BIOTECHNOLOGY, INC. pro-MCH (1D1): sc-293231 BACKGROUND APPLICATIONS Melanin-concentrating hormone (MCH) is a 19 amino acid cyclic neuropeptide pro-MCH (1D1) is recommended for detection of pro-MCH of human origin derived from a 165 amino acid pro-MCH precursor. In addition to the hormone, by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), the pro-MCH precursor contains a 144 amino acid mature MCH as well as a immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell 12 amino acid neuropeptide glycine-glutamic acid (NGE) and a 19 amino acid lysate)] and solid phase ELISA (starting dilution 1:30, dilution range 1:30- neuropeptide glutamic acid-isoleucine (NEI). Mainly expressed in the hypo- 1:3000). thalamus, the melanin-concentrating hormone modulates feeding behavior, Suitable for use as control antibody for pro-MCH siRNA (h): sc-42015, aggression, anxiety, arousal and reproductive function in mammals by con- pro-MCH shRNA Plasmid (h): sc-42015-SH and pro-MCH shRNA (h) trolling the release of luteinizing hormone (LH). The melanin-concentrating Lentiviral Particles: sc-42015-V. hormone receptor (MCHR, also designated SLC-1) is a glycosylated G protein- coupled receptor. MCHR mediates the effects of MCH through Gα i and/or Molecular Weight of pro-MCH: 45-50 kDa. Gα q signaling and is expressed in several regions of the brain, including the cerebral cortex, amygdala, thalamus and hypothalamus. MCH and MCHR RECOMMENDED SUPPORT REAGENTS have also been implicated in stimulating leptin expression and secretion in To ensure optimal results, the following support reagents are recommended: adipocytes, suggesting that the melanin-concentrating hormone and its 1) Western Blotting: use m-IgGk BP-HRP: sc-516102 or m-IgGk BP-HRP (Cruz receptor may be potential targets for modulating obesity.
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Activin-A Stimulates Hypothalamic Gonadotropin-Releasing Hormone Release by the Explanted Male Rat Hypothalamus: Interaction with Inhibin and Androgens
269 Activin-A stimulates hypothalamic gonadotropin-releasing hormone release by the explanted male rat hypothalamus: interaction with inhibin and androgens A E Calogero, N Burrello, A M Ossino, P Polosa and R D’Agata Division of Andrology, Department of Internal Medicine, University of Catania, 95123 Catania, Italy (Requests for offprints should be addressed to A E Calogero, Istituto di Medicina Interna e Specialita` Internistiche, Ospedale Garibaldi, Piazza S.M. di Gesu`, 95123 Catania, Italy) Abstract The presence of activins in those hypothalamic regions had no effect on GnRH output. As previously shown, containing gonadotropin-releasing hormone (GnRH)- testosterone (1 nmol/l) and dihydrotestosterone (DHT, secreting neurons suggests that these peptides may regulate 0·1 nmol/l) suppressed basal GnRH release, but only the reproductive function modulating not only pituitary testosterone was able to inhibit the release of GnRH FSH release and biosynthesis, but also hypothalamic stimulated by activin-A. Since DHT is a non-aromatizable GnRH release. The purpose of this study was to evaluate androgen, we evaluated whether the inhibitory effect of the effects of activin-A, a homodimer of inhibin âA testosterone was due to its in vitro conversion into 17â- subunit, on hypothalamic GnRH release in vitro and, estradiol. The addition of 4-hydroxyandrostenedione, a because of their well known antithetical effects, to evalu- steroidal aromatase inhibitor, did not influence the sup- ate its interaction with inhibin. In addition, since andro- pressive effect of testosterone on GnRH release stimulated gens modulate the release of GnRH from male rat by activin-A. hypothalami, we thought it of interest to study the possible In conclusion, activin-A stimulated hypothalamic interplay between these steroids and activin on GnRH GnRH release in vitro and this effect was abolished by release.
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Ing Hormone Receptor-11
Acta Pharmacol Sin 2008 Jun; 29 (6): 752–758 Full-length article High-throughput screening of novel antagonists on melanin-concentrat- ing hormone receptor-11 Jian-hua YAN2, Qun-yi LI2, Jean A BOUTIN3, M Pierre RENARD3, Yi-xiang DING4, Xiao-jiang HAO5, Wei-min ZHAO2, Ming- wei WANG2,6 2The National Center for Drug Screening and the State Key Laboratory of New Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; 3Les Laboratoires Servier, Neuilly-sur-Seine 92200, France; 4Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China; 5Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China Key words Abstract melanin-concentrating hormone; melanin- Aim: To find new antagonists on human melanin-concentrating hormone recep- concentrating hormone receptor-1; antagonist tor-1 (MCHR-1) through high-throughput screening (HTS) of a diverse com- 3 1Project supported in part by the Shanghai pound library. Methods: MCHR-1, [ H]SNAP7941, and FlashBlue G-protein- Municipality Science and Technology coupled receptor beads were used to measure the receptor-binding activities of Development Fund (No 06DZ22907 and various compounds based on scintillation proximity assay (SPA) technology. 07DZ22920), the Ministry of Science and 35 35 Technology (No 2004CB518902), and The guanosine 5' (γ-[ S]thio) triphosphate ([ S]GTPγS) binding assay was sub- Servier Beijing Pharmaceutical Research and sequently applied to functionally characterize the “hits” identified by the HTS Development Co, Ltd. campaign. Results: Of the 48 240 compounds screened with the SPA method, 6Correspondence to Dr Ming-wei WANG. Phn 86-21-5080-1313.
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Melanin-Concentrating Hormone Directly Inhibits Gnrh Neurons and Blocks Kisspeptin Activation, Linking Energy Balance to Reproduction
Melanin-concentrating hormone directly inhibits GnRH neurons and blocks kisspeptin activation, linking energy balance to reproduction Min Wua, Iryna Dumalskaa, Elena Morozovaa, Anthony van den Polb, and Meenakshi Alrejaa,c,1 Departments of aPsychiatry, bNeurosurgery, and cNeurobiology, Yale University School of Medicine and the Ribicoff Research Facilities, Connecticut Mental Health Center, New Haven, CT 06508 Edited by Wylie Vale, The Salk Institute for Biological Studies, La Jolla, CA, and approved August 18, 2009 (received for review July 29, 2009) A link between energy balance and reproduction is critical for the MCH neurons, which are mostly located in the lateral hypothal- survival of all species. Energy-consuming reproductive processes need amus and in the zona incerta, may also target GnRH neurons to be aborted in the face of a negative energy balance, yet knowledge directly, as MCH fibers are in close apposition with GnRH neurons of the pathways mediating this link remains limited. Fasting and food (22). MCH acts via the G-protein-coupled receptors MCHR1 restriction that inhibit fertility also upregulate the hypothalamic (23–27) and MCHR2 (28–30); only MCHR1 is present in the melanin-concentrating hormone (MCH) system that promotes feed- rodent brain, and 50–55% of rat GnRH neurons express MCHR1 ing and decreases energy expenditure; MCH knockout mice are lean (22). Intracerebral infusions of MCH can suppress (31) or enhance and have a higher metabolism but remain fertile. MCH also modulates (32, 33) pituitary gonadotropin release, depending on the estro- sleep, drug abuse behavior, and mood, and MCH receptor antagonists genic milieu. Fasting and food restriction, which has an inhibitory are currently being developed as antiobesity and antidepressant effect on fertility as evidenced by decreased circulating gonadotro- drugs.
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Inhibin and Activin Modulate the Release of Gonadotropin-Releasing Hormone, Human Chorionic Gonadotropin, and Progesterone From
Proc. Nati. Acad. Sci. USA Vol. 86, pp. 5114-5117, July 1989 Medical Sciences Inhibin and activin modulate the release of gonadotropin-releasing hormone, human chorionic gonadotropin, and progesterone from cultured human placental cells (foliculostatin/follicle-stimulating hormone-releasing protein/follitropin/foliicle-stimulating hormone) FELICE PETRAGLIA*, JOAN VAUGHAN, AND WYLIE VALEt Clayton Foundation Laboratories for Peptide Biology, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037 Communicated by C. H. Sawyer, March 20, 1989 (receivedfor review September 23, 1988) ABSTRACT Although it is clear that human chorionic cotropin], supports the hypothesis that placental hormono- gonadotropin (hCG) and progesterone play fundamental roles genesis may be regulated in part by locally produced peptides in pregnancy, the regulation of placental production of these (13-22). hormones remains to be defined. Recent evidence suggests that A recent report (11) showed that the addition of inhibin the human placenta expresses proteins related to inhibin (afi antiserum increased hCG production in primary human pla- subunits) or activin (8P subunits). Inhibin and activin (follicle- cental cultures, suggesting that endogenous inhibin might stimulating hormone-releasing protein) possess opposing ac- tonically inhibit secretion of the placental gonadotropin. To tivities in several biological systems including pituitary follicle- evaluate the possible roles of inhibin-related proteins in the stimulating hormone (follitropin) secretion, erythroid differ- regulation of placental hormones more directly, we have entiation, and gonadal sex-steroid production. The actions of investigated the effects-ofinhibin and activin on the secretion purified inhibin and activin on hormonogenesis by primary ofGnRH, hCG, and progesterone by cultured placental cells. cultures of human placental cells were studied.
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Activins and Inhibins: Roles in Development, Physiology, and Disease
Downloaded from http://cshperspectives.cshlp.org/ on October 5, 2021 - Published by Cold Spring Harbor Laboratory Press Activins and Inhibins: Roles in Development, Physiology, and Disease Maria Namwanje1 and Chester W. Brown1,2,3 1Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030 2Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030 3Texas Children’s Hospital, Houston, Texas 77030 Correspondence: [email protected] Since their original discovery as regulators of follicle-stimulating hormone (FSH) secretion and erythropoiesis, the TGF-b family members activin and inhibin have been shown to participate in a variety of biological processes, from the earliest stages of embryonic devel- opment to highly specialized functions in terminally differentiated cells and tissues. Herein, we present the history, structures, signaling mechanisms, regulation, and biological process- es in which activins and inhibins participate, including several recently discovered biolog- ical activities and functional antagonists. The potential therapeutic relevance of these advances is also discussed. INTRODUCTION, HISTORY AND which the activins and inhibins participate, rep- NOMENCLATURE resenting some of the most fascinating aspects of TGF-b family biology. We will also incorporate he activins and inhibins are among the 33 new biological activities that have been recently Tmembers of the TGF-b family and were ﬁrst discovered, the potential clinical relevance of described as regulators of follicle-stimulating
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Impact of the National Health Fund Policy on Hormone Treatment for Prostate Cancer in Jamaica
Investigación original / Original research Impact of the National Health Fund policy on hormone treatment for prostate cancer in Jamaica Belinda F. Morrison,1 William D. Aiken,1 and Marvin E. Reid 2 Suggested citation Morrison BF, Aiken WD, Reid ME. Impact of the National Health Fund policy on hormone treatment for prostate cancer in Jamaica. Rev Panam Salud Publica. 2011;29(6):404–8. ABSTRACT Objective. To compare the proportion of patients choosing surgical versus medical castra- tion to treat prostate cancer, before and after the National Health Fund (NHF) of Jamaica began to subsidize hormone therapy. Methods. A retrospective review was performed at the University Hospital of the West In- dies (UHWI), Jamaica. The pathology database at UHWI was searched to identify patients who had prostate biopsies between January 2000 and December 2007. These were combined with records of biopsies at external institutions. Medical records of all patients with positive prostate biopsies were reviewed to determine if they had received androgen deprivation ther- apy (ADT). Patients were classified as having had surgical castration (bilateral orchiectomy) or medical castration. Chi-square statistics were used to determine the difference in propor- tions between those choosing medical versus surgical castration before and after March 2005, when the NHF began offering subsidies for ADT drugs. Results. Of the 1 529 prostate biopsies performed during the study period, 680 (44.0%) cases of prostate cancer were diagnosed. Of these, 458 patients underwent ADT and had com- plete records available for analysis. The mean patient age was 72 years. During the entire study period, surgical castration was performed in 265 patients (58.0%) and medical castra- tion in 193 (42.0%).
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(FSH) and Luteinizing Hormone (LH) in Urine by Pubertal Girls
Pediat. Res. 6: 18-25 (1972) Follicle-stimulating Iuteinizing hormone hormone menarche gonadotropin ovulation Excretion of Follicle-Stimulating Hormone (FSH) and Luteinizing Hormone (LH) in Urine by Pubertal Girls ALLEN HAYES AND ANN JOHANSON1461 Department of Pediatrics, University of Virginia School of Medicine, Charlottesville, Virginia, USA Extract Daily urinary excretion of follicle-stimulating hormone (FSH) and luteinizing hor- mone (LH) in one premenarchial and four postmenarchial girls was determined over a 3-month collection period. Samples were collected overnight and a 24-hr excretion was extrapolated. Gonadotropins were acetone-precipitated and assessed by using the Second International Reference Preparation of Human Menopausal Gonadotropin (2nd IRP-HMG). Excretion of LH and FSH in the premenarchial girl followed no constant pattern, but average levels were comparable to postmenarchial and adult levels for FSH and early postmenarchial levels for LH. During the 3-month observation period, two simultaneous spikes of LH to 55 international units (IU)/24 hr and FSH to 60 IU/24 hr, occurring 6 days apart, were observed. These were of the magnitude of low normal adult midcyclc peak values. In all postmenarchial girls, simultaneous midcycle surges of FSII to as high as 70 IU/24 hr and LH to 43-155 IU/24 hr were observed in all cycles. During the earliest postmenarchial cycles, peak levels were less than those observed during cycles of girls who were postmenarchial for a longer period of time. Some characteristics of adult cycles, such as follicular phase FSH elevations and higher follicular than luteal phase levels of both gonadotropins, were observed during cycles occurring only 1-4 months after menarche.
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The Roles of Melanin-Concentrating Hormone in Energy Balance and Reproductive Function: Are They Connected?
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