Postdoctoral Position in Signal Transduction GMI - Gregor Mendel Institute of Molecular Plant Biology / Vienna, Austria

Total Page：16

File Type：pdf, Size：1020Kb

Postdoctoral Position in Signal Transduction GMI - Gregor Mendel Institute of Molecular Plant Biology / Vienna, Austria Research in our group focuses on dissecting fundamental molecular processes at the interface between signal transduction and coordinated responses of cellular metabolism and gene expression in stress situations. In our recent work we established the Arabidopsis protein kinase ASKalfa as a novel regulator of stress tolerance (Dal Santo et al.,2012, Plant Cell, 24:3380). We now wish to identify relevant upstream regulators and additional novel targets of ASKalfa and are therefore looking for a creative, flexible, and enthusiastic new team member interested in basic molecular mechanisms of signal transduction and physiological responses to stress. The GMI (http://www.gmi.oeaw.ac.at) is located at the “Campus Vienna Biocenter”, a cluster of research institutes and biotech companies (http://www.viennabiocenter.org/). GMI's research activities are supported by a world-class infrastructure including state-of- the-art plant growth facilities, mass spectrometry, biooptics and next generation sequencing. Vienna is a vibrant international city that ranks as one of the most attractive cities world wide. The successful candidate should have a strong background in biochemistry and/or molecular biology. Experience in protein kinase work and/or metabolic analysis is desirable. Practical knowledge of Arabidopsis physiology and/or genetics would certainly be a big advantage. Initially, the project is planned to last two years with a possible extension. To apply, please send a letter of motivation briefly outlining your ideas about the project, a full CV including a brief description of research experience and interests, and the contact details of at least two referees via email to: Claudia Jonak phone: + 43 1 79044 9850 email: [email protected] .

Copy Link

Recommended publications

Structural Basis for Receptor Recognition of Pollen Tube Attraction Peptides
ARTICLE DOI: 10.1038/s41467-017-01323-8 OPEN Structural basis for receptor recognition of pollen tube attraction peptides Xiaoxiao Zhang1, Weijia Liu1, Takuya T. Nagae2, Hidenori Takeuchi3, Heqiao Zhang1, Zhifu Han1, Tetsuya Higashiyama2,4,5 & Jijie Chai1,6,7 Transportation of the immobile sperms directed by pollen tubes to the ovule-enclosed female gametophytes is important for plant sexual reproduction. The defensin-like (DEFL) cysteine- 1234567890 rich peptides (CRPs) LUREs play an essential role in pollen tube attraction to the ovule, though their receptors still remain controversial. Here we provide several lines of biochemical evidence showing that the extracellular domain of the leucine-rich repeat receptor kinase (LRR-RK) PRK6 from Arabidopsis thaliana directly interacts with AtLURE1 peptides. Structural study reveals that a C-terminal loop of the LRR domain (AtPRK6LRR) is responsible for recognition of AtLURE1.2, mediated by a set of residues largely conserved among PRK6 homologs from Arabidopsis lyrata and Capsella rubella, supported by in vitro mutagenesis and semi-in-vivo pollen tube growth assays. Our study provides evidence showing that PRK6 functions as a receptor of the LURE peptides in A. thaliana and reveals a unique ligand recognition mechanism of LRR-RKs. 1 Ministry of Education Key Laboratory of Protein Science, Center for Structural Biology, School of Life Sciences, Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University, 100084 Beijing, China. 2 Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan. 3 Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna, Austria.
[Show full text]
Director, Vienna Biocenter Core Facilities
Director, Vienna BioCenter Core Facilities Location: Vienna, Austria The Vienna BioCenter (VBC), http://viennabiocenter.org/ ,is one of the leading multidisciplinary biomedical research centers in Europe and the premier location for Life Sciences in Austria. Its research institutions include the Institute of Molecular Pathology (IMP), the Institute of Molecular Biotechnology (IMBA), the Gregor Mendel Institute (GMI), and the Max F. Perutz Laboratories (MFPL). The VBC further hosts 17 biotech companies. The VBC has attracted excellent scientists from 70 different nations as well as substantial private and public funding. The Vienna BioCenter Core Facilities (VBCF) GmbH, http://www.vbcf.ac.at/, provides research infrastructure to researchers at the VBC and beyond. It currently employs a staff of 80 and is funded by a grant from the Austrian Science Ministry, the City of Vienna and user fees. Since its foundation in 2010, VBCF has succeeded in implementing a broad range of outstanding infrastructure, to recruit highly-qualified experts, and to develop a unique portfolio of research services. In parallel, VBCF has become a flagship for cutting-edge technologies essential for top-level research in Vienna. Responsibilities The Director is responsible for overseeing and coordinating all scientific, technology and management aspects of the VBCF core facilities in interaction with the shareholders and the funding bodies. Main Tasks • Leadership of the VBCF and definition of long-term objectives of the VBCF in dialogue with the VBC stakeholders
[Show full text]
Ping Kao and Michael Nodine
© 2021. Published by The Company of Biologists Ltd | Development (2021) 148, dev199884. doi:10.1242/dev.199884 INTERVIEW The people behind the papers – Ping Kao and Michael Nodine The application of single-cell mRNA sequencing technologies to plant embryos promises to reveal the gene expression dynamics underlying cell-type differentiation. A new paper in Development reports the generation of high-quality transcriptomes from single embryonic nuclei without contamination from maternal tissues. To find out more about the story, we caught up with first author Ping Kao and his supervisor Michael Nodine, who recently moved from the Gregor Mendel Institute in Vienna to become Assistant Professor in the Laboratory of Molecular Biology at Wageningen University in the Netherlands. Michael, can you give us your scientific biography and the questions your lab is trying to answer? MN: My first research position was at the Clemson University Genomics Institute in South Carolina starting in 2000, and then at the Arizona Genomics Institute with Professor Rod Wing. This was followed by PhD training in genomics, molecular and cellular biology at the University of Arizona in Professor Frans Tax’s lab. Ping (L) and Michael (R) in front of the Belvedere in Vienna (image credit: I then joined Professor Dave Bartel’s lab at the Whitehead Institute Nicholas Nodine). for Biomedical Research at MIT, where my interest in RNA biology grew and fused with the fascination with plant embryology and PhD programme, we have been actively searching for new solutions genomics I had developed as a student. In the summer of 2012, to improve our knowledge of zygotic embryos.
[Show full text]
Genome-Wide Association Studies Were Pioneered by Human
PRESS ALERT From weeds to humans: new mixed model GWAS approach developed from Arabidopsis data opens up new analytic possibilities, also for human genetics. Using the model plant Arabidopsis thaliana, Magnus Nordborg, Scientific Director of the Gregor Mendel Institute of Molecular Plant Biology of the Austrian Academy of Sciences, Arthur Korte, and other colleagues at the GMI have extended the mixed model GWAS approach to now also deal with correlated phenotypes. This development has exciting potential for analysis not only of plant but also of human genetic data; it will also allow for cost-efficient re-analysis of data from previous studies. The results of this study have been published in the current online issue of the scientific journal Nature Genetics: “A mixed-model approach for genome-wide association studies of correlated traits in structured population.” A fundamental challenge in biology today is understanding the respective contributions that the environment and the genetic makeup of an organism, plant or animal, make to its phenotypic (physically manifested) traits. GWAS (genome-wide association studies) attempt to understand how genetic variation translates into phenotypic variation. The principle behind a genome-wide association study is to compare the DNA sequences of individual organisms and to see whether particular differences in the DNA sequence are associated with particular differences in the characteristics of the individuals. However, relationships between individuals in a population present a major difficulty; to solve this problem, mixed models have been recently developed. This new study now extends this mixed model approach to also deal with correlated phenotypic traits. Being able to deal with these correlations will allow new insights into the interplay between environment and genetic makeup.
[Show full text]
Cytogenetic Mapping of Common Bean Chromosomes Reveals a Less Compartmentalized Small-Genome Plant Species
Chromosome Research (2009) 17:405–417 DOI 10.1007/s10577-009-9031-4 Cytogenetic mapping of common bean chromosomes reveals a less compartmentalized small-genome plant species Andrea Pedrosa-Harand & James Kami & Paul Gepts & Valérie Geffroy & Dieter Schweizer Received: 13 November 2008 /Revised and Accepted: 21 January 2009 / Published online: 28 March 2009 # The Author(s) 2009. This article is published with open access at Springerlink.com Abstract Cytogenetic maps of common bean chro- with genetically mapped markers, mostly with single- mosomes 3, 4 and 7 were constructed by fluorescence copy RFLPs, a large subset of BACs, from 13 in-situ hybridization (FISH) of BAC and a few other different genomic regions, contained repetitive genomic clones. Although all clones were selected sequences, as concluded from the regional distribu- tion patterns of multiple FISH signals on chromo- somes: pericentromeric, subtelomeric and dispersed. Electronic supplementary material The online version of this article (doi:10.1007/s10577-009-9031-4) contains Pericentromeric repeats were present in all 11 chro- supplementary material, which is available to authorized users. mosome pairs with different intensities, whereas subtelomeric repeats were present in several chromo- Responsible Editor: Jiming Jiang. some ends, but with different signal intensities : A. Pedrosa-Harand D. Schweizer depending on the BAC, suggesting that the terminal Department of Chromosome Biology, University of Vienna, heterochromatin fraction of this species may be Vienna, Austria composed of different repeats. The correlation of J. Kami : P. Gepts genetic and physical distances along the three studied Department of Plant Sciences/MS1, chromosomes was obtained for 23 clones. This Section of Crop and Ecosystem Sciences, correlation suggests suppression of recombination University of California, around extended pericentromeric regions in a similar Davis, CA, USA way to that previously reported for plant species with V.
[Show full text]
Director, Vienna Biocenter Core Facilities Location: Vienna, Austria
Director, Vienna BioCenter Core Facilities Location: Vienna, Austria The Vienna BioCenter (VBC), http://viennabiocenter.org/ ,is one of the leading multidisciplinary biomedical research centers in Europe and the premier location for Life Sciences in Austria. Its research institutions include the Institute of Molecular Pathology (IMP), the Institute of Molecular Biotechnology (IMBA), the Gregor Mendel Institute (GMI), and the Max F. Perutz Laboratories (MFPL). The VBC further hosts 17 biotech companies. The VBC has attracted excellent scientists from 70 different nations as well as substantial pri- vate and public funding. The Vienna BioCenter Core Facilities (VBCF) GmbH, http://www.vbcf.ac.at/, provides research infrastructure to researchers at the VBC and beyond. It currently employs a staff of 80 and is funded by a grant from the Austrian Science Ministry, the City of Vienna and user fees. Since its foundation in 2010, VBCF has succeeded in implementing a broad range of outstanding infrastructure, to recruit highly-qualified experts, and to develop a unique portfolio of research services. In parallel, VBCF has become a flagship for cutting-edge technologies essential for top-level research in Vienna. Responsibilities The Director is responsible for overseeing and coordinating all scientific, technology and management aspects of the VBCF core facilities in interaction with the shareholders and the funding bodies. Main Tasks • Leadership of the VBCF and definition of long-term objectives of the VBCF in dialogue with the VBC stakeholders
[Show full text]
Gregor Mendel Institute of Molecular Plant Biology, GMI
14 Gregor Mendel Institute of Molecular Plant Biology, GMI Head: Dieter Schweizer Aims and Functions Research at the GMI is curiosity driven and currently The Gregor Mendel Institute of Molecular Plant Biology focuses on the genetic and epigenetic plasticity of the (GMI GmbH) was founded by the Austrian Academy of plant genome in the contexts of gene regulation, chro- Sciences in 2000 to promote research excellence within mosome biology and development. We share a common the field of plant molecular biology. The GMI is the first interest in epigenetics with the IMP and IMBA. GMI and only international centre for basic plant research in scientists also study the nature and crosstalk of plant sig- Austria. Since January 2006, it has been located at the nal transduction pathways in response to intrinsic and Vienna Biocenter Campus – which encompasses both environmental stimuli at both the genetic and epigenetic independent and academic research institutes and com- levels. Arabidopsis thaliana is used as the primary model panies. One of the GMI’s great strengths lies in its prox- organism. Research groups are evaluated annually by an imity to institutions undertaking biomedical research. international Scientific Advisory Board. Research at the We share a building, the Austrian Academy of Sciences GMI is supported primarily by the Austrian Academy of Life Sciences Center Vienna, with the Institute of Mo- Sciences, complemented by grants obtained from various lecular Biotechnology (IMBA) and are internally con- funding agencies. In the years 2004/2005, GMI group nected to the adjacent Research Institute of Molecular leaders received external grants from the Austrian Sci- Pathology (IMP) of Boehringer Ingelheim.
[Show full text]
Ku Is Required for Telomeric C-Rich Strand Maintenance but Not for End-To-End Chromosome Fusions in Arabidopsis
Ku is required for telomeric C-rich strand maintenance but not for end-to-end chromosome fusions in Arabidopsis Karel Riha* and Dorothy E. Shippen† Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX 77843-2128 Edited by Joseph G. Gall, Carnegie Institution of Washington, Baltimore, MD, and approved November 21, 2002 (received for review October 9, 2002) Telomere dysfunction arising from mutations in telomerase or in Higher eukaryotes respond differently to a deficiency in Ku. telomere capping proteins leads to end-to-end chromosome fu- Mouse mutants lacking Ku exhibit premature senescence and sions. Paradoxically, the Ku70͞80 heterodimer, essential for non- stunted growth (19, 20). At the genome level, frequent end-to- homologous end-joining double-strand break repair, is also found end chromosome fusions and perturbations in telomere length at telomeres, and in mammals it is required to prevent telomere homeostasis are observed (21–24). We previously showed that fusion. Previously, we showed that inactivation of Ku70 in Arabi- telomeres in Arabidopsis mutants lacking Ku70 are dramatically dopsis results in telomere lengthening. Here, we have demon- extended, but their capping function seems to be intact, as we strated that this telomere elongation is telomerase dependent. found no evidence for chromosome fusions (9). ؅ Further, we found that the terminal 3 G overhang was signiﬁ- To further investigate the behavior of Ku in Arabidopsis,we cantly extended in ku70 mutants and in plants deﬁcient in both analyzed telomere architecture and maintenance in Ku70 mu- Ku70 and the catalytic subunit of telomerase (TERT), implying that tants and in mutants deficient in both Ku70 and telomerase.
[Show full text]
2009 Austria MASC Report
Austria http://www.arabidopsis.org/portals/masc/countries/Austria.jsp Epigenetics: Contact: Marie-Theres Hauser Werner Aufsatz (www.gmi.oeaw.ac.at/waufsatz.htm): RNA BOKU-University of Natural Resources & Applied Life mediated silencing, stress adaptation Science, Vienna Antonius and Marjori Matzke (www.gmi.oeaw.ac.at/amatzke. Email: [email protected] htm): RdDM, nuclear architecture Ortrun Mittelsten Scheid (www.gmi.oeaw.ac.at/oms.htm): In Austria, Arabidopsis projects are undertaken at four epigenetic changes in polyploids institutions (BOKU-University of Natural Resources & Applied Life Science Vienna, GMI-Gregor Mendel Institute of Hisashi Tamaru (www.gmi.oeaw.ac.at/htamaru.htm): Molecular Plant Biology, MFPL-Max F. Perutz Laboratories, chromatin during pollen development University of Salzburg) on: Glycobiology: Population genetics: Georg Seifert (www.dapp.boku.ac.at/ips.html?&L=1): Magnus Nordborg (www.gmi.oeaw.ac.at/en/research/magnus- arabinogalactan proteins and PCD nordborg/) has been appointed as director of the GMI Herta Steinkellner (www.dagz.boku.ac.at/11132.html?&L=1): Systems biology: “customised” N-glycosylation Wolfram Weckwerth has been newly appointed as Head of the Richard Strasser (www.dagz.boku.ac.at/12349.html?&L=1): Molecular Systems Biology at the University of Vienna N-glycosylation Chromosome biology: Raimund Tenhaken (www.uni-salzburg.at/zbio/tenhaken): biosynthesis of nucleotide sugars for cell wall polymers, PCD Karel Riha (www.gmi.oeaw.ac.at/rkriha.htm): telomeres and genome stability Plant
[Show full text]
Arabidopsis Thaliana Functional Genomics Project Annual Report 2008
The Multinational Coordinated Arabidopsis thaliana Functional Genomics Project Annual Report 2008 Xing Wang Deng [email protected] Chair Joe Kieber [email protected] Co-chair Joanna Friesner [email protected] MASC Coordinator and Executive Secretary Thomas Altmann [email protected] Sacha Baginsky [email protected] Ruth Bastow [email protected] Philip Benfey [email protected] David Bouchez [email protected] Jorge Casal [email protected] Danny Chamovitz [email protected] Bill Crosby [email protected] Joe Ecker [email protected] Klaus Harter [email protected] Marie-Theres Hauser [email protected] Pierre Hilson [email protected] Eva Huala [email protected] Jaakko Kangasjärvi [email protected] Julin Maloof [email protected] Sean May [email protected] Peter McCourt [email protected] Harvey Millar [email protected] Ortrun Mittelsten- Scheid [email protected] Basil Nikolau [email protected] Javier Paz-Ares [email protected] Chris Pires [email protected] Barry Pogson [email protected] Ben Scheres [email protected] Randy Scholl [email protected] Heiko Schoof [email protected] Kazuo Shinozaki [email protected] Klaas van Wijk [email protected] Paola Vittorioso [email protected] Wolfram Weckwerth [email protected] Weicai Yang [email protected] The Multinational Arabidopsis Steering Committee—July 2008 Front Cover Design Philippe Lamesch, Curator at TAIR/Carnegie Institute for Science, and Joanna Friesner, MASC Coordinator at the University of California, Davis, USA Images Map of geographical distribution of ecotypes of Arabidopsis thaliana Updated representation contributed by Philippe Lamesch, adapted from Jonathon Clarke, UK (1993).
[Show full text]
Meiosis & Telomeres
Katedry Genetiky a Bioché mie Prírodovedeckej fakulty Univerzity Komenské ho Vá s pozývajú na 45. prednášku v rá mci Kuž elových seminárov: Dr. Karel ŘÍHA Gregor Mendel Institute of Molecular Plant Biology Austrian Academy of Sciences, Viedeň , Rakúsko Meiosis & Telomeres: Unexpected connections ktorá sa uskutoč ní v piatok 12. novembra 2004 o 14:00 v miestnosti B1-501PriF UK http://www.fns.uniba.sk/~kbi/kuzela Dr. Karel ŘÍHA http://www.gmi.oeaw.ac.at/rkriha.htm 1990-1995: M.Sc. in Molecular Biology and genetics, Masaryk University, Brno, Czech republic 1995-1998: Ph.D. in Genetics, Masaryk University, Brno, Czech republic 1995-1998: Graduate research assistant in laboratory of Dr. Boris Vyskot, Institute of Biophysics, Czech Academy of Sciences, Brno, Czech Republic. 1999-2002: Postdoctoral research associate in laboratory of Dr. Dorothy E. Shippen at Department of Biochemistry & Biophysics, Texas A&M University, College Station, USA. 2003-present: Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Vienna, Austria. Lecture background: Telomeres are nucleoprotein structures which protect chromosome ends from being recognized as DNA double-strand breaks (DSB) and processed by DNA repair machinery. Interestingly, several DNA repair complexes appear to be essential for telomere protection and processing. Our research is focused on investigating the function of DNA repair proteins in telomere maintenance using the genetically tractable plant model Arabidopsis thaliana. We are particularly interested in the Ku70/80 heterodimer, which is important for the DSB repair via the non-homologous end-joining pathway as well as for telomere protection. Another area of our interest is the role of telomeres in genome stability.
[Show full text]
The Other Genome Project
Charis Cook The Other Genome Project n this article, Charis Cook explains the Arabidopsis thaliana is a model species like the fruit Key words significance of a cress called Arabidopsis, fly Drosophila, yeast, and the bacterium Escherichia coli. Scientists work on these species in order to genome the plant with the best-understood genome. understand as much as possible about a particular Arabidopsis I kind of organism. Arabidopsis was first suggested Food security hit the headlines in the 1960s, when database as a plant model species for developmental and leading economists were predicting a catastrophic genetic research in 1943. A German scientist Green Revolution food crisis. That food-based apocalypse never named Friedrich Laibach noted that A. thaliana arrived due to the unforeseen Green Revolution was easy to grow inside in a small space, showed which was largely down to a long 20 years of work a lot of natural variation, was amenable to cross- by Nobel Peace Prize winner Norman Borlaug. breeding between varieties, and generated large Today, global population growth and climate numbers of seeds per plant. change means the world’s food supply is again facing an uncertain future. Fortunately, plant research has changed beyond recognition since Borlaug started his wheat breeding programme in 1944, and scientific progress moves much faster today. The reason for the step change in plant science is extensive research into an insignificant member of the cabbage family, Arabidopsis thaliana, subject of The Other Genome Project. Envel Kerdaffrec, Gregor Mendel Institute Arabidopsis ecotypes: A selection of Arabidopsis ecotypes growing at the 1001 Genomes Project at the Gregor Mendel Institute, Austria.
[Show full text]