Plant Epigenetics: from Mechanisms to Ecological Relevance
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45Th Anniversary Year
VOLUME 45, NO. 1 Spring 2021 Journal of the Douglasia WASHINGTON NATIVE PLANT SOCIETY th To promote the appreciation and 45 conservation of Washington’s native plants Anniversary and their habitats through study, education, Year and advocacy. Spring 2021 • DOUGLASIA Douglasia VOLUME 45, NO. 1 SPRING 2021 journal of the washington native plant society WNPS Arthur R. Kruckberg Fellows* Clay Antieau Lou Messmer** President’s Message: William Barker** Joe Miller** Nelsa Buckingham** Margaret Miller** The View from Here Pamela Camp Mae Morey** Tom Corrigan** Brian O. Mulligan** by Keyna Bugner Melinda Denton** Ruth Peck Ownbey** Lee Ellis Sarah Reichard** Dear WNPS Members, Betty Jo Fitzgerald** Jim Riley** Mary Fries** Gary Smith For those that don’t Amy Jean Gilmartin** Ron Taylor** know me I would like Al Hanners** Richard Tinsley Lynn Hendrix** Ann Weinmann to introduce myself. I Karen Hinman** Fred Weinmann grew up in a small town Marie Hitchman * The WNPS Arthur R. Kruckeberg Fellow Catherine Hovanic in eastern Kansas where is the highest honor given to a member most of my time was Art Kermoade** by our society. This title is given to Don Knoke** those who have made outstanding spent outside explor- Terri Knoke** contributions to the understanding and/ ing tall grass prairie and Arthur R. Kruckeberg** or preservation of Washington’s flora, or woodlands. While I Mike Marsh to the success of WNPS. Joy Mastrogiuseppe ** Deceased love the Midwest, I was ready to venture west Douglasia Staff WNPS Staff for college. I earned Business Manager a Bachelor of Science Acting Editor Walter Fertig Denise Mahnke degree in Wildlife Biol- [email protected] 206-527-3319 [email protected] ogy from Colorado State Layout Editor University, where I really Mark Turner Office and Volunteer Coordinator [email protected] Elizabeth Gage got interested in native [email protected] plants. -
Conserving Europe's Threatened Plants
Conserving Europe’s threatened plants Progress towards Target 8 of the Global Strategy for Plant Conservation Conserving Europe’s threatened plants Progress towards Target 8 of the Global Strategy for Plant Conservation By Suzanne Sharrock and Meirion Jones May 2009 Recommended citation: Sharrock, S. and Jones, M., 2009. Conserving Europe’s threatened plants: Progress towards Target 8 of the Global Strategy for Plant Conservation Botanic Gardens Conservation International, Richmond, UK ISBN 978-1-905164-30-1 Published by Botanic Gardens Conservation International Descanso House, 199 Kew Road, Richmond, Surrey, TW9 3BW, UK Design: John Morgan, [email protected] Acknowledgements The work of establishing a consolidated list of threatened Photo credits European plants was first initiated by Hugh Synge who developed the original database on which this report is based. All images are credited to BGCI with the exceptions of: We are most grateful to Hugh for providing this database to page 5, Nikos Krigas; page 8. Christophe Libert; page 10, BGCI and advising on further development of the list. The Pawel Kos; page 12 (upper), Nikos Krigas; page 14: James exacting task of inputting data from national Red Lists was Hitchmough; page 16 (lower), Jože Bavcon; page 17 (upper), carried out by Chris Cockel and without his dedicated work, the Nkos Krigas; page 20 (upper), Anca Sarbu; page 21, Nikos list would not have been completed. Thank you for your efforts Krigas; page 22 (upper) Simon Williams; page 22 (lower), RBG Chris. We are grateful to all the members of the European Kew; page 23 (upper), Jo Packet; page 23 (lower), Sandrine Botanic Gardens Consortium and other colleagues from Europe Godefroid; page 24 (upper) Jože Bavcon; page 24 (lower), Frank who provided essential advice, guidance and supplementary Scumacher; page 25 (upper) Michael Burkart; page 25, (lower) information on the species included in the database. -
Structural Basis for Receptor Recognition of Pollen Tube Attraction Peptides
ARTICLE DOI: 10.1038/s41467-017-01323-8 OPEN Structural basis for receptor recognition of pollen tube attraction peptides Xiaoxiao Zhang1, Weijia Liu1, Takuya T. Nagae2, Hidenori Takeuchi3, Heqiao Zhang1, Zhifu Han1, Tetsuya Higashiyama2,4,5 & Jijie Chai1,6,7 Transportation of the immobile sperms directed by pollen tubes to the ovule-enclosed female gametophytes is important for plant sexual reproduction. The defensin-like (DEFL) cysteine- 1234567890 rich peptides (CRPs) LUREs play an essential role in pollen tube attraction to the ovule, though their receptors still remain controversial. Here we provide several lines of biochemical evidence showing that the extracellular domain of the leucine-rich repeat receptor kinase (LRR-RK) PRK6 from Arabidopsis thaliana directly interacts with AtLURE1 peptides. Structural study reveals that a C-terminal loop of the LRR domain (AtPRK6LRR) is responsible for recognition of AtLURE1.2, mediated by a set of residues largely conserved among PRK6 homologs from Arabidopsis lyrata and Capsella rubella, supported by in vitro mutagenesis and semi-in-vivo pollen tube growth assays. Our study provides evidence showing that PRK6 functions as a receptor of the LURE peptides in A. thaliana and reveals a unique ligand recognition mechanism of LRR-RKs. 1 Ministry of Education Key Laboratory of Protein Science, Center for Structural Biology, School of Life Sciences, Tsinghua-Peking Joint Center for Life Sciences, Tsinghua University, 100084 Beijing, China. 2 Division of Biological Science, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan. 3 Gregor Mendel Institute (GMI), Austrian Academy of Sciences, Vienna Biocenter (VBC), Dr. Bohr-Gasse 3, 1030 Vienna, Austria. -
Director, Vienna Biocenter Core Facilities
Director, Vienna BioCenter Core Facilities Location: Vienna, Austria The Vienna BioCenter (VBC), http://viennabiocenter.org/ ,is one of the leading multidisciplinary biomedical research centers in Europe and the premier location for Life Sciences in Austria. Its research institutions include the Institute of Molecular Pathology (IMP), the Institute of Molecular Biotechnology (IMBA), the Gregor Mendel Institute (GMI), and the Max F. Perutz Laboratories (MFPL). The VBC further hosts 17 biotech companies. The VBC has attracted excellent scientists from 70 different nations as well as substantial private and public funding. The Vienna BioCenter Core Facilities (VBCF) GmbH, http://www.vbcf.ac.at/, provides research infrastructure to researchers at the VBC and beyond. It currently employs a staff of 80 and is funded by a grant from the Austrian Science Ministry, the City of Vienna and user fees. Since its foundation in 2010, VBCF has succeeded in implementing a broad range of outstanding infrastructure, to recruit highly-qualified experts, and to develop a unique portfolio of research services. In parallel, VBCF has become a flagship for cutting-edge technologies essential for top-level research in Vienna. Responsibilities The Director is responsible for overseeing and coordinating all scientific, technology and management aspects of the VBCF core facilities in interaction with the shareholders and the funding bodies. Main Tasks • Leadership of the VBCF and definition of long-term objectives of the VBCF in dialogue with the VBC stakeholders -
Ping Kao and Michael Nodine
© 2021. Published by The Company of Biologists Ltd | Development (2021) 148, dev199884. doi:10.1242/dev.199884 INTERVIEW The people behind the papers – Ping Kao and Michael Nodine The application of single-cell mRNA sequencing technologies to plant embryos promises to reveal the gene expression dynamics underlying cell-type differentiation. A new paper in Development reports the generation of high-quality transcriptomes from single embryonic nuclei without contamination from maternal tissues. To find out more about the story, we caught up with first author Ping Kao and his supervisor Michael Nodine, who recently moved from the Gregor Mendel Institute in Vienna to become Assistant Professor in the Laboratory of Molecular Biology at Wageningen University in the Netherlands. Michael, can you give us your scientific biography and the questions your lab is trying to answer? MN: My first research position was at the Clemson University Genomics Institute in South Carolina starting in 2000, and then at the Arizona Genomics Institute with Professor Rod Wing. This was followed by PhD training in genomics, molecular and cellular biology at the University of Arizona in Professor Frans Tax’s lab. Ping (L) and Michael (R) in front of the Belvedere in Vienna (image credit: I then joined Professor Dave Bartel’s lab at the Whitehead Institute Nicholas Nodine). for Biomedical Research at MIT, where my interest in RNA biology grew and fused with the fascination with plant embryology and PhD programme, we have been actively searching for new solutions genomics I had developed as a student. In the summer of 2012, to improve our knowledge of zygotic embryos. -
Genome-Wide Association Studies Were Pioneered by Human
PRESS ALERT From weeds to humans: new mixed model GWAS approach developed from Arabidopsis data opens up new analytic possibilities, also for human genetics. Using the model plant Arabidopsis thaliana, Magnus Nordborg, Scientific Director of the Gregor Mendel Institute of Molecular Plant Biology of the Austrian Academy of Sciences, Arthur Korte, and other colleagues at the GMI have extended the mixed model GWAS approach to now also deal with correlated phenotypes. This development has exciting potential for analysis not only of plant but also of human genetic data; it will also allow for cost-efficient re-analysis of data from previous studies. The results of this study have been published in the current online issue of the scientific journal Nature Genetics: “A mixed-model approach for genome-wide association studies of correlated traits in structured population.” A fundamental challenge in biology today is understanding the respective contributions that the environment and the genetic makeup of an organism, plant or animal, make to its phenotypic (physically manifested) traits. GWAS (genome-wide association studies) attempt to understand how genetic variation translates into phenotypic variation. The principle behind a genome-wide association study is to compare the DNA sequences of individual organisms and to see whether particular differences in the DNA sequence are associated with particular differences in the characteristics of the individuals. However, relationships between individuals in a population present a major difficulty; to solve this problem, mixed models have been recently developed. This new study now extends this mixed model approach to also deal with correlated phenotypic traits. Being able to deal with these correlations will allow new insights into the interplay between environment and genetic makeup. -
Literature Cited Robert W. Kiger, Editor This Is a Consolidated List Of
RWKiger 26 Jul 18 Literature Cited Robert W. Kiger, Editor This is a consolidated list of all works cited in volumes 24 and 25. In citations of articles, the titles of serials are rendered in the forms recommended in G. D. R. Bridson and E. R. Smith (1991). When those forms are abbreviated, as most are, cross references to the corresponding full serial titles are interpolated here alphabetically by abbreviated form. Two or more works published in the same year by the same author or group of coauthors will be distinguished uniquely and consistently throughout all volumes of Flora of North America by lower-case letters (b, c, d, ...) suffixed to the date for the second and subsequent works in the set. The suffixes are assigned in order of editorial encounter and do not reflect chronological sequence of publication. The first work by any particular author or group from any given year carries the implicit date suffix "a"; thus, the sequence of explicit suffixes begins with "b". Works missing from any suffixed sequence here are ones cited elsewhere in the Flora that are not pertinent in these volumes. Aares, E., M. Nurminiemi, and C. Brochmann. 2000. Incongruent phylogeographies in spite of similar morphology, ecology, and distribution: Phippsia algida and P. concinna (Poaceae) in the North Atlantic region. Pl. Syst. Evol. 220: 241–261. Abh. Senckenberg. Naturf. Ges. = Abhandlungen herausgegeben von der Senckenbergischen naturforschenden Gesellschaft. Acta Biol. Cracov., Ser. Bot. = Acta Biologica Cracoviensia. Series Botanica. Acta Horti Bot. Prag. = Acta Horti Botanici Pragensis. Acta Phytotax. Geobot. = Acta Phytotaxonomica et Geobotanica. [Shokubutsu Bunrui Chiri.] Acta Phytotax. -
Cytogenetic Mapping of Common Bean Chromosomes Reveals a Less Compartmentalized Small-Genome Plant Species
Chromosome Research (2009) 17:405–417 DOI 10.1007/s10577-009-9031-4 Cytogenetic mapping of common bean chromosomes reveals a less compartmentalized small-genome plant species Andrea Pedrosa-Harand & James Kami & Paul Gepts & Valérie Geffroy & Dieter Schweizer Received: 13 November 2008 /Revised and Accepted: 21 January 2009 / Published online: 28 March 2009 # The Author(s) 2009. This article is published with open access at Springerlink.com Abstract Cytogenetic maps of common bean chro- with genetically mapped markers, mostly with single- mosomes 3, 4 and 7 were constructed by fluorescence copy RFLPs, a large subset of BACs, from 13 in-situ hybridization (FISH) of BAC and a few other different genomic regions, contained repetitive genomic clones. Although all clones were selected sequences, as concluded from the regional distribu- tion patterns of multiple FISH signals on chromo- somes: pericentromeric, subtelomeric and dispersed. Electronic supplementary material The online version of this article (doi:10.1007/s10577-009-9031-4) contains Pericentromeric repeats were present in all 11 chro- supplementary material, which is available to authorized users. mosome pairs with different intensities, whereas subtelomeric repeats were present in several chromo- Responsible Editor: Jiming Jiang. some ends, but with different signal intensities : A. Pedrosa-Harand D. Schweizer depending on the BAC, suggesting that the terminal Department of Chromosome Biology, University of Vienna, heterochromatin fraction of this species may be Vienna, Austria composed of different repeats. The correlation of J. Kami : P. Gepts genetic and physical distances along the three studied Department of Plant Sciences/MS1, chromosomes was obtained for 23 clones. This Section of Crop and Ecosystem Sciences, correlation suggests suppression of recombination University of California, around extended pericentromeric regions in a similar Davis, CA, USA way to that previously reported for plant species with V. -
Director, Vienna Biocenter Core Facilities Location: Vienna, Austria
Director, Vienna BioCenter Core Facilities Location: Vienna, Austria The Vienna BioCenter (VBC), http://viennabiocenter.org/ ,is one of the leading multidisciplinary biomedical research centers in Europe and the premier location for Life Sciences in Austria. Its research institutions include the Institute of Molecular Pathology (IMP), the Institute of Molecular Biotechnology (IMBA), the Gregor Mendel Institute (GMI), and the Max F. Perutz Laboratories (MFPL). The VBC further hosts 17 biotech companies. The VBC has attracted excellent scientists from 70 different nations as well as substantial pri- vate and public funding. The Vienna BioCenter Core Facilities (VBCF) GmbH, http://www.vbcf.ac.at/, provides research infrastructure to researchers at the VBC and beyond. It currently employs a staff of 80 and is funded by a grant from the Austrian Science Ministry, the City of Vienna and user fees. Since its foundation in 2010, VBCF has succeeded in implementing a broad range of outstanding infrastructure, to recruit highly-qualified experts, and to develop a unique portfolio of research services. In parallel, VBCF has become a flagship for cutting-edge technologies essential for top-level research in Vienna. Responsibilities The Director is responsible for overseeing and coordinating all scientific, technology and management aspects of the VBCF core facilities in interaction with the shareholders and the funding bodies. Main Tasks • Leadership of the VBCF and definition of long-term objectives of the VBCF in dialogue with the VBC stakeholders -
Gregor Mendel Institute of Molecular Plant Biology, GMI
14 Gregor Mendel Institute of Molecular Plant Biology, GMI Head: Dieter Schweizer Aims and Functions Research at the GMI is curiosity driven and currently The Gregor Mendel Institute of Molecular Plant Biology focuses on the genetic and epigenetic plasticity of the (GMI GmbH) was founded by the Austrian Academy of plant genome in the contexts of gene regulation, chro- Sciences in 2000 to promote research excellence within mosome biology and development. We share a common the field of plant molecular biology. The GMI is the first interest in epigenetics with the IMP and IMBA. GMI and only international centre for basic plant research in scientists also study the nature and crosstalk of plant sig- Austria. Since January 2006, it has been located at the nal transduction pathways in response to intrinsic and Vienna Biocenter Campus – which encompasses both environmental stimuli at both the genetic and epigenetic independent and academic research institutes and com- levels. Arabidopsis thaliana is used as the primary model panies. One of the GMI’s great strengths lies in its prox- organism. Research groups are evaluated annually by an imity to institutions undertaking biomedical research. international Scientific Advisory Board. Research at the We share a building, the Austrian Academy of Sciences GMI is supported primarily by the Austrian Academy of Life Sciences Center Vienna, with the Institute of Mo- Sciences, complemented by grants obtained from various lecular Biotechnology (IMBA) and are internally con- funding agencies. In the years 2004/2005, GMI group nected to the adjacent Research Institute of Molecular leaders received external grants from the Austrian Sci- Pathology (IMP) of Boehringer Ingelheim. -
Phylogeny, Morphology and the Role of Hybridization As Driving Force Of
bioRxiv preprint doi: https://doi.org/10.1101/707588; this version posted July 18, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Phylogeny, morphology and the role of hybridization as driving force of evolution in 2 grass tribes Aveneae and Poeae (Poaceae) 3 4 Natalia Tkach,1 Julia Schneider,1 Elke Döring,1 Alexandra Wölk,1 Anne Hochbach,1 Jana 5 Nissen,1 Grit Winterfeld,1 Solveig Meyer,1 Jennifer Gabriel,1,2 Matthias H. Hoffmann3 & 6 Martin Röser1 7 8 1 Martin Luther University Halle-Wittenberg, Institute of Biology, Geobotany and Botanical 9 Garden, Dept. of Systematic Botany, Neuwerk 21, 06108 Halle, Germany 10 2 Present address: German Centre for Integrative Biodiversity Research (iDiv), Deutscher 11 Platz 5e, 04103 Leipzig, Germany 12 3 Martin Luther University Halle-Wittenberg, Institute of Biology, Geobotany and Botanical 13 Garden, Am Kirchtor 3, 06108 Halle, Germany 14 15 Addresses for correspondence: Martin Röser, [email protected]; Natalia 16 Tkach, [email protected] 17 18 ABSTRACT 19 To investigate the evolutionary diversification and morphological evolution of grass 20 supertribe Poodae (subfam. Pooideae, Poaceae) we conducted a comprehensive molecular 21 phylogenetic analysis including representatives from most of their accepted genera. We 22 focused on generating a DNA sequence dataset of plastid matK gene–3'trnK exon and trnL– 23 trnF regions and nuclear ribosomal ITS1–5.8S gene–ITS2 and ETS that was taxonomically 24 overlapping as completely as possible (altogether 257 species). -
Formalized Classification of Ephemeral Wetland Vegetation
Formalized classification of ephemeral wetland vegetation (Isoëto-Nanojuncetea class) in Poland (Central Europe) Zygmunt Kącki1,*, Andrzej Łysko2, Zygmunt Dajdok3, Piotr Kobierski4, Rafał Krawczyk5, Arkadiusz Nowak6, Stanisław Rosadzinski 7,8 and Agnieszka Anna Popiela9,* 1 Botanical Garden, University of Wrocław, Wrocław, Poland 2 Faculty of Computer Science and Information Technology, Western Pomeranian University of Technology in Szczecin, Szczecin, Poland 3 Institute of Environmental Biology, University of Wrocław, Wrocław, Poland 4 Zespół Szkół Technicznych im. Władysława Reymonta w Lubsku, Lubsko, Poland 5 Institute of Biological Sciences, Maria Curie-Skłodowska University, Lublin, Poland 6 Botanical Garden, Center for Biological Diversity Conservation, Polish Academy of Sciences, Warszawa, Poland 7 Department of Plant Ecology and Environmental Protection, Faculty of Biology, Adam Mickiewicz University in Poznan, Poznan, Poland 8 Current Affiliation: Poznan, Poland 9 Institute of Biology, University of Szczecin, Szczecin, Poland * These authors contributed equally to this work. ABSTRACT Formalized classification of the class Isoëto-Nanojuncetea has not been performed in Poland. We used 69,562 relevés stored in Polish Vegetation Database. Based on the literature and expert knowledge we selected 63 diagnostic species for the Isoëto- Nanojuncetea class. Unequivocal classification was applied in this work according to Cocktail method. A set of formal definitions was established using a combination of logical operators of total cover of species in case of high-rank syntaxa while sociological species groups and cover of particular species were used for logical formulas describing class, alliances and associations. An Expert System was prepared Submitted 26 November 2020 and applied to classify the whole data set of PVD and 1,340 relevés were organized at Accepted 8 June 2021 the class level.