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Regional Research Station (Terai Zone) Directorate of Research Uttar Banga Krishi Viswavidyalaya Pundibari, Cooch Behar, West Bengal, Pin-736165

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Regional Research Station (Terai Zone) Directorate of Research Uttar Banga Krishi Viswavidyalaya Pundibari, Cooch Behar, West Bengal, Pin-736165 WHERE WISDOM IS FREE REGIONAL RESEARCH STATION (TERAI ZONE) DIRECTORATE OF RESEARCH UTTAR BANGA KRISHI VISWAVIDYALAYA PUNDIBARI, COOCH BEHAR, WEST BENGAL, PIN-736165 Contents Sl. Title Pages No. 1 Preface 2 2 Research programme undertaken in Kharif-2017-18 3-85 3 Research programme undertaken in Rabi-2017-18 86-153 4 Personal information of the scientists 154- 170 i. Total staff of RRSTZ 154 ii Award 155 iii Memberships 156- 157 iv Project other than RRSTZ 158- 162 v Publication other than RRSTZ 163- 168 vi Publication from RRSTZ 169 vii Seminar and symposium 170 With an objective of catering the location specific research need of sub Himalayan plains of the State, the Regional Research Station for Terai Zone was established in 1978 under the then newly formed North Bengal Campus of Bidhan Chandra Krishi Viswavidyalaya (BCKV) at Pundibari, Cooch Behar. Subsequently blessed with human resource support from National Agricultural Research Project (NARP) in 1990, the research activity of the Station achieved a strategic boost through formulation of bench level study of the zone. The goal setting was done on the basis of identified rationales and the task was accomplished by dedicated participation of multi- disciplinary group of scientists each committed to his/her own field. In 2001, the Station extended adequate support in formulation of new Directorate of Research of Uttar Banga Krishi Viswavidyalaya (UBKV) born out of bifurcation of BCKV and since then it became a constituent of the same. This also incurred some static change in scientists group through optional discontinuity of few seniors and subsequent refilling by fresher. Under the present era of UBKV, the station observes due responsibility to keep parity with the predecessors vis-a-vis setting new goals pertaining with existing agro ecological dynamics of the zone. Based upon the research need of this area under consideration programmes are adopted and carried out accordingly on seasonal basis twice in a year i.e. Rabi and Kharif. Apart from half yearly project formulation session, Scientists meet regularly for technical discussion on progress of running programmes once in every month and all issues related with same are covered duly. The present Report comprises of results obtained out of programmes under taken at the Station for three consecutive crop seasons i.e. Pre Kharif , Kharif 2017and Rabi 2017-18. Critical observations, suggestions on this report will be cordially accepted for further improvement of the same. Prof. Ashutosh Sarkar In-Charge, RRS (Terai Zone) Uttar Banga Krishi Viswavidyalaya Pundibari, Cooch Behar 736165 List of Kharif-2017-18 Programme under RRSTZ Sl. Project Code Title of the Programme Associate Scientists No. Animal Science & Fishery 1 RRSTZ/Kharif/17- Studies on the prevalence of the major Dr. Nonigopal Shit 18/04 vector borne diseases affecting cross breed Dr. Dilip Kumar Hajra cattle in Cooch Behar district, West Bengal 2 RRSTZ/2017- Improved induced breeding program on Prof. Debapriya Sarkar 2018/Kharif/05 Asian magur through administration and Dr. Nilanjana inducing hormone and different synthetic Chaudhuri analogues. 3 RRSTZ/2017- Performance trial of different crops with Prof. Debapriya Sarkar 2018/Kharif/06 fish under Integrated Farming System and Dr. Nilanjana Research in the fish farm of RRS, Terai Chaudhuri Zone Crop Improvement 4 RRSTZ/2017- Selection of mungbean genotypes for Dr. Rupsanatan Mandal 2018/Kharif/03 tolerance to water-logging under field condition Crop Production 5 RRSTZ/Kharif/17- Project Title: Improved package of Dr. Parthendu Poddar, 18/02 practices for major Agricultural and Dr. Suchand Datta, Dr. Horticultural crops in Terai Zone of West Partha Sarathi Patra Bengal. Crop Protection 6 RRSTZ/Kharif/17- Project Title: A Review on the Occurrence Dr. Suprakash Pal, Dr. 18/01 of Pests and Diseases of major crops and Nilanjana Choudhuri, Their Management in Terai Region of West Dr. Satyajit Hembram Bengal Annual Technical Report 2017-18 ANIMAL SCIENCE AND FISHERIES Title of the Programme: Studies on the prevalence of the major vector borne diseases affecting cross breed cattle in Cooch Behar district, West Bengal Project code: RRSTZ/Kharif/17-18/04 Associate Scientist/s: Dr. Nonigopal Shit & Dr. Dilip Kumar Hajra Objectives: The investigation of the prevalence of vector borne haemoprotozoan diseases in cross breed cattle population in Cooch Behar district Figure 1: Blood collection at the peripheral circulation and thin smear preparation from the different areas of Cooch Behar district Methodology of the programme: Sample collection: Blood collection (at least 5ml) was aseptically made from the jugular vein or peripheral circulation (ear vein) of the target animals in an anti-coagulant coated sterile sample collection vial labelled previously as required. Immediately after collection, sample was temporarily kept in the ice-pack insulated box and brought cautiously to the laboratory without hemolysis. Figure 3: Microscopic examination (100X) of haemoprotozoan parasites of cross bred cattle in Cooch Behar district 3 Annual Technical Report 2017-18 ANIMAL SCIENCE AND FISHERIES Smear preparation and centrifugation: A small drop of fresh blood was mounted over a dry and grease free slide and using another slide of smooth edge, a thin blood smear was made in triplicate. The rest blood was centrifuged following standard protocol (3000 rpm for 10min) for the serum extraction. The serum was emptied to the serum collection cryo-vials suitably labelled and stored at -18oC for further biochemical study. Staining and Microscopic examination: Glass slides with thin blood smear were air dried and fixed with methanol (75%). Consequent to that glass slides were kept on the staining rack and the smear was stained with diluted Giemsa’s stain (Himedia Pvt. Ltd) following to manufacture’s instruction. The stain was allowed for 15min and finally washed under slow tap water till the stain is removed. The smear was air dried completely and each slide was examined under oil emersion microscope (100 x illuminations) and counted the total number of parasites from at least fifty fields of each slide. Figure 2: Giemsa’s staining of dry blood smear at the laboratory, animal science unit for the prevalence study of haemoprotozoan parasites Results: The study was focused to examine the occurrence of the haemo-protozoan parasites in the cross bred cattle under Cooch Behar district. Among the total 200 samples, only 28, 5, 13 and 2 samples were recorded positive for the Theileriosis, Babesiosis, Anaplasmosis and Trypanosomiasis respectively. On the percent prevalence basis, Theileriosis (T. annulata) revealed highest degree of prevalence (14%) and the least infection was counted for Trypanosomiasis (T. evansi). 4 Annual Technical Report 2017-18 ANIMAL SCIENCE AND FISHERIES Prevalence 14 12 10 8 6 Prevalent % Prevalent 4 2 0 Theileria Babesia Anaplasma Trypanosoma Figure 4: Prevalent percent of the haemo-protozoan parasites recorded in the cross bred cattle in Cooch Behar district 200 200 175 150 125 positive samples 100 75 50 28 13 5 No. Of positiveOf samples No. 25 2 0 Total sample Theileria Babesia Anaplasma Trypanosoma Figure 5: Positive samples of different haemo-protozoan parasites in the randomly collected blood of cross bred cattle in Cooch Behar district 5 Annual Technical Report 2017-18 ANIMAL SCIENCE AND FISHERIES Figure 6: Identification of the intra-cellular haemoprotozoan parasites under oil emersion microscopic examination The questionnaire survey prior to this study demonstrated in spite of the proper management animals suffers from the chronic problems of anorexia, laboured breathing, gradual loss of production and susceptibility to diseases even in minor changes of climatic condition. The present findings could be invariably correlated with these facts and facets which are prevailed over the field condition. Biochemical analysis may correlate more precisely to reach at pin point diagnosis. Further study including DNA based molecular diagnosis of the antigen for those parasites will be more valuable for concrete year round recommendations 6 Annual Technical Report 2017-18 ANIMAL SCIENCE AND FISHERIES Title of the Programme: Improved induced breeding program on Asian magur through administration inducing hormone and different synthetic analogues. Project code: RRSTZ/2017-2018/Kharif/05 Associate Scientist/s: Prof. Debapriya Sarkar and Dr. Nilanjana Chaudhuri Objectives: Assessment on comparative efficacy of ALFA-FH (synthetic hormone) and pituitary extract (natural hormone) on stripping method of induced breeding in Asian magur. Comparison betweennumber of hatchlings produced from stripping (sacrifice) and non-sacrifice method of induced breeding. Interpretation on cost analysis for both ways. EXPERIMENTAL DESIGN Experiment No. 1. Title : Comparative efficacy of ALFA-FH and pituitary extract on stripping method of induced breeding in Clarius batrachus. Methodology : Five sets (males : females = 2:1) of brood Asian magur each weighing 200-250g were separately injected with ALFA-FH and pituitary hormone @ 0.4 ml/100 g and 4mg/100g body wt of female respectively. Stripping method were followed for egg collection from females after 16 hrs Sperm fluid were collected through sacrificing the males Two males were used to fertilize eggs collected from one female Sticky fertilized eggs were kept in aluminium tray with medium water flow to get hatchlings within 24 hours (Fig.1) Hatchlings were fed after 72 hrs with artemia as recommended for 15 days and later on with egg custard or boiled rice and eggs to attain a length of 2 inches. Observations : Table 1. Summary table of Asian magur breeding with ALFA-FH and Pituitary extract ALFA-FH PITUITARY EXTRACT Av.wt of female (g) 200 g 200 g Av.wt of male (g) 250 g 250 g Dose in female 0.4 ml/100gbw 4 mg/ 100 g bw Dose in male 0.2 ml/fish 2 mg / fish Av. no of eggs released 2650 nos/100g 4500 nos/100g No of hatchlings 1000 nos/100g 2500 nos/100g 7 Annual Technical Report 2017-18 ANIMAL SCIENCE AND FISHERIES Interpretation : Table 1 clearly indicated a better performance by pituitary extract as compared with ALFA- FH as inducing agent in stripping method.
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