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Product Information Sheet for HM-1099 Eggerthella sp., Strain MVA1 Tryptic soy agar with 5% defibrinated sheep blood or Modified Chopped Meat agar with 0.5% arginine or equivalent Incubation: Catalog No. HM-1099 Temperature: 37°C Atmosphere: Anaerobic For research use only. Not for human use. Propagation: 1. Keep vial frozen until ready for use, then thaw. Contributor: 2. Transfer the entire thawed aliquot into a single tube of broth. Maria V. Sizova, Department of Biology, Northeastern 3. Use several drops of the suspension to inoculate an agar University, Boston, Massachusetts, USA slant and/or plate. 4. Incubate the tube, slant and/or plate at 37°C for 2 to 4 days. Manufacturer: BEI Resources Citation: Acknowledgment for publications should read “The following Product Description: reagent was obtained through BEI Resources, NIAID, NIH as Bacteria Classification: Eggerthellaceae, Eggerthella part of the Human Microbiome Project: Eggerthella sp., Strain Species: Eggerthella sp. MVA1, HM-1099.” Strain: MVA1 Original Source: Eggerthella sp., strain MVA1 is a vaginal Biosafety Level: 2 isolate obtained in 2011 from a woman with bacterial Appropriate safety procedures should always be used with this vaginosis in Seattle, Washington, USA.1,2 material. Laboratory safety is discussed in the following Comments: Eggerthella sp., strain MVA1 (HMP ID 1636) is a publication: U.S. Department of Health and Human Services, reference genome for The Human Microbiome Project (HMP). Public Health Service, Centers for Disease Control and HMP is an initiative to identify and characterize human Prevention, and National Institutes of Health. Biosafety in microbial flora. The complete genome of Microbiological and Biomedical Laboratories. 5th ed. Eggerthella sp., strain MVA1 is being sequenced at the Washington, DC: U.S. Government Printing Office, 2009; see J. Craig Venter Institute. www.cdc.gov/biosafety/publications/bmbl5/index.htm. Note: HMP material is taxonomically classified by the depositor. Quality control of these materials is only performed to Disclaimers: demonstrate that the material distributed by BEI Resources is You are authorized to use this product for research use only. It identical to the deposited material. is not intended for human use. Eggerthella species are Gram-positive, obligately anaerobic, Use of this product is subject to the terms and conditions of the non-sporulating, non-motile rods found in the large intestine and BEI Resources Material Transfer Agreement (MTA). The MTA genitourinary tract.3 Eggerthella were reclassified from is available on our Web site at www.beiresources.org. Eubacteria in 1999, and the family Eggerthellaceae was reclassified from Coriobacteriaceae in 2013.3-5 Eggerthella While BEI Resources uses reasonable efforts to include species are phenotypically difficult to identify, and are emerging accurate and up-to-date information on this product sheet, as human pathogens.6,7 neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific Material Provided: literature and patents are provided for informational purposes Each vial contains approximately 0.5 mL of bacterial culture in only. Neither ATCC® nor the U.S. Government warrants that Modified Chopped Meat medium with 0.5% arginine such information has been confirmed to be accurate. supplemented with 10% glycerol. This product is sent with the condition that you are responsible Note: If homogeneity is required for your intended use, please for its safe storage, handling, use and disposal. ATCC® and the purify prior to initiating work. U.S. Government are not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable Packaging/Storage: effort is made to ensure authenticity and reliability of materials ® HM-1099 was packaged aseptically in cryovials. The product is on deposit, the U.S. Government, ATCC , their suppliers and provided frozen and should be stored at -60°C or colder contributors to BEI Resources are not liable for damages arising immediately upon arrival. For long-term storage, the vapor from the misidentification or misrepresentation of products. phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided. Use Restrictions: This material is distributed for internal research, Growth Conditions: non-commercial purposes only. This material, its product or Media: its derivatives may not be distributed to third parties. Except as Modified Chopped Meat medium with 0.5% arginine or performed under a U.S. Government contract, individuals equivalent contemplating commercial use of the material, its products or its BEI Resources E-mail: [email protected] www.beiresources.org Tel: 800-359-7370 Fax: 703-365-2898 © 2018 American Type Culture Collection (ATCC). All rights reserved. HM-1099_26MAY2018 Page 1 of 2 Product Information Sheet for HM-1099 derivatives must contact the contributor to determine if a license is required. U.S. Government contractors may need a license before first commercial sale. References: 1. Sizova, M. V., Personal Communication. 2. HMP ID 1636 (Eggerthella sp., strain MVA1) 3. Moore, W. E. C., E. P. Cato and L. V. Holdeman. “Eubacterium lenturn (Eggerth) Prevot 1938: Emendation of Description and Designation of the Neotype Strain.” Int. J. Syst. Evol. Microbiol. 21 (1971): 299-303. 4. Gupta, R. S., et al. “Molecular Signatures for the Class Coriobacteriia and its Different Clades; Proposal for Division of the Class Coriobacteriia into the Emended Order Coriobacteriales, Containing the Emended Family Coriobacteriaceae and Atopobiaceae fam. nov., and Eggerthellales ord. nov., Containing the Family Eggerthellaceae fam. nov.” Int. J. Syst. Evol. Microbiol. 63 (2013): 3379-3397. PubMed: 23524353. 5. Kageyama, A., Y. Benno and T. Nakase. “Phylogenetic Evidence for the Transfer of Eubacterium lentum to the Genus Eggerthella as Eggerthella lenta gen. nov., comb. nov.” Int. J. Syst. Bacteriol. 49 (1999): 1725-1732. PubMed: 10555354. 6. Cho, G. S., et al. “Quantification of Slackia and Eggerthella spp. in Human Feces and Adhesion of Representatives Strains to Caco-2 Cells.” Front. Microbiol. 7 (2016): 658. PubMed: 27242689. 7. Ling, Z., et al. “Molecular Analysis of the Diversity of Vaginal Microbiota Associated with Bacterial Vaginosis.” BMC Genomics. 11 (2010): 488. PubMed: 20819230. ATCC® is a trademark of the American Type Culture Collection. BEI Resources E-mail: [email protected] www.beiresources.org Tel: 800-359-7370 Fax: 703-365-2898 © 2018 American Type Culture Collection (ATCC). All rights reserved. HM-1099_26MAY2018 Page 2 of 2 .
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  • Supplementary Data TMAO Microbiome R1

    Supplementary Data TMAO Microbiome R1

    BMJ Publishing Group Limited (BMJ) disclaims all liability and responsibility arising from any reliance Supplemental material placed on this supplemental material which has been supplied by the author(s) Gut Content for Supplementary Data Tables Tables Page Annotation guide for taxonomic features. 1 Supplementary Data 1a. Microbial associations with TMAO, and intakes 7 of choline and red meat. Supplementary Data 1b. Microbial associations with TMAO in 8 15 sensitivity analysis models. Supplementary Data 2a. Associations of intakes of choline and red meat, 21 or other TMAO precursors with TMAO levels, stratified by TMAO- associated abundant species, in full models and additionally adjusted for other TMAO precursors or TMAO predicting species. Supplementary Data 2b. Associations of intakes of choline and red meat, 34 or other TMAO precursors with TMAO levels, stratified by TMAO- producer status, additionally adjusted for other TMAO precursors. Supplementary Data 2c. Associations of intakes of choline and red meat, 37 or other TMAO precursors with TMAO levels, stratified by TMAO- producer status, in 6 different sensitivity analysis models. Supplementary Data 2d. Associations of intakes of red meat with HDLC 39 and HBA1c levels, stratified by TMAO-producer status or TMAO- associated abundant species. Supplementary Data 3a. Association of DNA gene clusters within 40 TMAO-associated species with plasma TMAO levels, and intakes of choline and red meat. Supplementary Data 3b. Association of transcriptions of gene clusters 46 (RNA/DNA ratio) within TMAO-associated species with plasma TMAO levels, and intakes of choline and red meat. Supplementary Data 4a. Association of DNA enzyme within TMAO- 62 associated species with plasma TMAO levels, and intakes of choline and red meat.