Transcriptomic and epigenomics atlas of myotubes reveals insight into the circadian control of metabolism and development Altnta, Ali; Laker, Rhianna C; Garde, Christian; Barrès, Romain; Zierath, Juleen R Published in: Epigenomics DOI: 10.2217/epi-2019-0391 Publication date: 2020 Document version Publisher's PDF, also known as Version of record Document license: CC BY-NC-ND Citation for published version (APA): Altnta, A., Laker, R. C., Garde, C., Barrès, R., & Zierath, J. R. (2020). Transcriptomic and epigenomics atlas of myotubes reveals insight into the circadian control of metabolism and development. Epigenomics, 12(8), 701- 713. https://doi.org/10.2217/epi-2019-0391 Download date: 25. Sep. 2021 Research Article For reprint orders, please contact:
[email protected] Transcriptomic and epigenomics atlas of myotubes reveals insight into the circadian control of metabolism and development Ali Altıntas¸‡,1 , Rhianna C Laker‡,1, Christian Garde1, Romain Barres` 1 & Juleen R Zierath*,1,2 1Section for Integrative Physiology, The Novo Nordisk Foundation Center for Basic Metabolic Research, University of Copenhagen, Copenhagen, Denmark 2Section for Integrative Physiology, Department of Molecular Medicine & Surgery, Karolinska Institutet, Stockholm, Sweden *Author for correspondence:
[email protected] ‡Authors contributed equally Aim: Innate circadian rhythms are critical for optimal tissue-specific functions, including skeletal muscle, a major insulin-sensitive tissue responsible for glucose homeostasis. We determined whether transcriptional oscillations are associated with CpG methylation changes in skeletal muscle. Materials & methods: We performed rhythmicity analysis on the transcriptome and CpG methylome of circadian synchronized my- otubes. Results: We identified several transcripts and CpG-sites displaying oscillatory behavior, which were enriched with Gene Ontology terms related to metabolism and development.