DOCSLIB.ORG

3 1 0 Material Safety Data Sheet

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text

Load more

He a lt h 1 3 Fire 3 1 0 Re a c t iv it y 0 Pe rs o n a l Pro t e c t io n H Material Safety Data Sheet Universal Indicator Solution MSDS Section 1: Chemical Product and Company Identification Product Name: Universal Indicator Solution Contact Information: Catalog Codes: SLU1051 Sciencelab.com, Inc. 14025 Smith Rd. CAS#: Mixture. Houston, Texas 77396 RTECS: Not applicable. US Sales: 1-800-901-7247 International Sales: 1-281-441-4400 TSCA: TSCA 8(b) inventory: Isopropyl alcohol; Methyl alcohol; Phenolphthalein; Bromothymol blue sodium salt; Order Online: ScienceLab.com Thymol blue, sodium salt; Methyl red, sodium salt; Water CHEMTREC (24HR Emergency Telephone), call: CI#: Not applicable. 1-800-424-9300 Synonym: International CHEMTREC, call: 1-703-527-3887 Chemical Name: Not applicable. For non-emergency assistance, call: 1-281-441-4400 Chemical Formula: Not applicable. Section 2: Composition and Information on Ingredients Composition: Name CAS # % by Weight Isopropyl alcohol 67-63-0 37 Methyl alcohol 67-56-1 0.8 Phenolphthalein 77-09-8 0.03 Bromothymol blue sodium salt 34722-90-2 0.03 Thymol blue, sodium salt 81012-93-3 0.03 Methyl red, sodium salt 845-10-3 0.02 Water 7732-18-5 62.1 Toxicological Data on Ingredients: Isopropyl alcohol: ORAL (LD50): Acute: 5045 mg/kg [Rat]. 3600 mg/kg [Mouse]. DERMAL (LD50): Acute: 12800 mg/kg [Rabbit]. VAPOR (LC50): Acute: 22627.4 ppm 4 hour(s) [Rat]. Methyl alcohol: ORAL (LD50): Acute: 5628 mg/kg [Rat.]. DERMAL (LD50): Acute: 15800 mg/kg [Rabbit.]. VAPOR (LC50): Acute: 64000 ppm 4 hour(s) [Rat.]. Section 3: Hazards Identification Potential Acute Health Effects: p. 1 Very hazardous in case of eye contact (irritant), of ingestion. Hazardous in case of skin contact (irritant), of inhalation. Slightly hazardous in case of skin contact (permeator). Non-corrosive for skin. Non-sensitizer for skin. Inflammation of the eye is characterized by redness, watering, and itching. Potential Chronic Health Effects: Non-corrosive for skin. Non-sensitizer for skin. Non-permeator by skin. CARCINOGENIC EFFECTS: Not available. MUTAGENIC EFFECTS: Not available. TERATOGENIC EFFECTS: Not available. DEVELOPMENTAL TOXICITY: PROVEN [Methyl alcohol] The substance is toxic to kidneys, lungs, the nervous system, mucous membranes, the reproductive system. Repeated or prolonged exposure to the substance can produce target organs damage. Section 4: First Aid Measures Eye Contact: Check for and remove any contact lenses. Immediately flush eyes with running water for at least 15 minutes, keeping eyelids open. Cold water may be used. Do not use an eye ointment. Seek medical attention. Skin Contact: After contact with skin, wash immediately with plenty of water. Gently and thoroughly wash the contaminated skin with running water and non-abrasive soap. Be particularly careful to clean folds, crevices, creases and groin. Cold water may be used. Cover the irritated skin with an emollient. If irritation persists, seek medical attention. Wash contaminated clothing before reusing. Serious Skin Contact: Wash with a disinfectant soap and cover the contaminated skin with an anti-bacterial cream. Seek medical attention. Inhalation: Allow the victim to rest in a well ventilated area. Seek immediate medical attention. Serious Inhalation: Evacuate the victim to a safe area as soon as possible. Loosen tight clothing such as a collar, tie, belt or waistband. If breathing is difficult, administer oxygen. If the victim is not breathing, perform mouth-to-mouth resuscitation. Seek medical attention. Ingestion: Do not induce vomiting. Loosen tight clothing such as a collar, tie, belt or waistband. If the victim is not breathing, perform mouth-to-mouth resuscitation. Seek immediate medical attention. Serious Ingestion: Not available. Section 5: Fire and Explosion Data Flammability of the Product: Flammable. Auto-Ignition Temperature: The lowest known value is 399°C (750.2°F) (Isopropyl alcohol). Flash Points: The lowest known value is CLOSED CUP: 11.67°C (53°F). (TAG). OPEN CUP: 18.3°C (64.9°F). (Cleveland). (Isopropyl alcohol) Flammable Limits: The greatest known range is LOWER: 2% UPPER: 12% (Isopropyl alcohol) Products of Combustion: These products are carbon oxides (CO, CO2). Fire Hazards in Presence of Various Substances: Flammable in presence of open flames and sparks, of oxidizing materials. Slightly flammable to flammable in presence of heat, of combustible materials. Explosion Hazards in Presence of Various Substances: Risks of explosion of the product in presence of mechanical impact: Not available. Risks of explosion of the product in presence of static discharge: Not available. Slightly explosive to explosive in presence of oxidizing materials. Fire Fighting Media and Instructions: Flammable liquid, soluble or dispersed in water. SMALL FIRE: Use DRY chemical powder. LARGE FIRE: Use alcohol foam, water spray or fog. p. 2 Special Remarks on Fire Hazards: Explosive in the form of vapor when exposed to heat or flame. May form explosive mixtures with air. Vapor may travel considerable distance to source of ignition and flash back. CAUTION: MAY BURN WITH NEAR INVISIBLE FLAME (Isopropyl alcohol) Special Remarks on Explosion Hazards: Not available. Section 6: Accidental Release Measures Small Spill: Dilute with water and mop up, or absorb with an inert dry material and place in an appropriate waste disposal container. Large Spill: Flammable liquid. Keep away from heat. Keep away from sources of ignition. Stop leak if without risk. Absorb with DRY earth, sand or other non-combustible material. Do not touch spilled material. Prevent entry into sewers, basements or confined areas; dike if needed. Eliminate all ignition sources. Be careful that the product is not present at a concentration level above TLV. Check TLV on the MSDS and with local authorities. Section 7: Handling and Storage Precautions: Keep away from heat. Keep away from sources of ignition. Ground all equipment containing material. Do not ingest. Do not breathe gas/fumes/ vapour/spray. In case of insufficient ventilation, wear suitable respiratory equipment If ingested, seek medical advice immediately and show the container or the label. Avoid contact with skin and eyes Storage: Flammable materials should be stored in a separate safety storage cabinet or room. Keep away from heat. Keep away from sources of ignition. Keep container tightly closed. Keep in a cool, well-ventilated place. Ground all equipment containing material. A refrigerated room would be preferable for materials with a flash point lower than 37.8°C (100°F). Section 8: Exposure Controls/Personal Protection Engineering Controls: Provide exhaust ventilation or other engineering controls to keep the airborne concentrations of vapors below their respective threshold limit value. Ensure that eyewash stations and safety showers are proximal to the work-station location. Personal Protection: Splash goggles. Lab coat. Vapor respirator. Be sure to use an approved/certified respirator or equivalent. Gloves. Personal Protection in Case of a Large Spill: Splash goggles. Full suit. Vapor respirator. Boots. Gloves. A self contained breathing apparatus should be used to avoid inhalation of the product. Suggested protective clothing might not be sufficient; consult a specialist BEFORE handling this product. Exposure Limits: Isopropyl alcohol TWA: 400 STEL: 500 (ppm) from ACGIH (TLV) [1995] TWA: 980 STEL: 1230 (mg/m3) from ACGIH [1995] Methyl alcohol TWA: 200 STEL: 250 (ppm) from ACGIH (TLV) [1995] TWA: 262 STEL: 328 (mg/m3) from ACGIH [1995] Consult local authorities for acceptable exposure limits. Section 9: Physical and Chemical Properties Physical state and appearance: Liquid. Odor: Not available. Taste: Not available. p. 3 Molecular Weight: Not applicable. Color: Not available. pH (1% soln/water): Neutral. Boiling Point: The lowest known value is 82.22°C (180°F) (Isopropyl alcohol). Weighted average: 93.36°C (200°F) Melting Point: May start to solidify at -89.5°C (-129.1°F) based on data for: Isopropyl alcohol. Critical Temperature: Not available. Specific Gravity: Weighted average: 0.91 (Water = 1) Vapor Pressure: The highest known value is 33 mm of Hg (@ 20°C) (Isopropyl alcohol). Weighted average: 23.31 mm of Hg (@ 20°C) Vapor Density: The highest known value is 2.07 (Air = 1) (Isopropyl alcohol). Weighted average: 1.16 (Air = 1) Volatility: Not available. Odor Threshold: The highest known value is 50 ppm (Isopropyl alcohol) Water/Oil Dist. Coeff.: The product is equally soluble in oil and water. Ionicity (in Water): Not available. Dispersion Properties: Is not dispersed in methanol, diethyl ether. See solubility in water, methanol, diethyl ether, n-octanol, acetone. Solubility: Easily soluble in cold water, hot water, methanol, diethyl ether, n-octanol, acetone. Section 10: Stability and Reactivity Data Stability: The product is stable. Instability Temperature: Not available. Conditions of Instability: Not available. Incompatibility with various substances: Slightly reactive to reactive with oxidizing agents. Corrosivity: Non-corrosive in presence of glass. Special Remarks on Reactivity: Not available. Special Remarks on Corrosivity: Not available. Polymerization: No. Section 11: Toxicological Information Routes of Entry: Eye contact. Inhalation. Ingestion. Toxicity to Animals: WARNING: THE LC50 VALUES HEREUNDER ARE ESTIMATED ON THE BASIS OF A 4-HOUR EXPOSURE. Acute oral toxicity (LD50): 9730 mg/kg (Mouse) (Calculated value for the mixture). Acute dermal toxicity (LD50): 34595 mg/kg (Rabbit) (Calculated value for the
Recommended publications
  • Technical Options for the Mitigation of Direct Methane and Nitrous Oxide Emissions from Livestock: a Review

    Technical Options for the Mitigation of Direct Methane and Nitrous Oxide Emissions from Livestock: a Review

    Animal (2013), 7:s2, pp 220–234 & Food and Agriculture Organization of the United Nations 2013 animal doi:10.1017/S1751731113000876 Technical options for the mitigation of direct methane and nitrous oxide emissions from livestock: a review - P. J. Gerber1 , A. N. Hristov2, B. Henderson1, H. Makkar1,J.Oh2, C. Lee2, R. Meinen2, F. Montes3,T.Ott2, J. Firkins4, A. Rotz5, C. Dell5, A. T. Adesogan6,W.Z.Yang7, J. M. Tricarico8, E. Kebreab9, G. Waghorn10, J. Dijkstra11 and S. Oosting11 1Agriculture and Consumer protection Department, Food and Agriculture Organization of the United Nations, Vialle delle terme di Caracalla, 00153 Rome, Italy; 2Department of Animal Science, The Pennsylvania State University, University Park, PA 16802, USA; 3Plant Science Department, The Pennsylvania State University, University Park, PA 16802, USA; 4Department of Animal Sciences, The Ohio State University, Columbus OH 43210, USA; 5Department of Animal Sciences, USDA-Agricultural Research Service, Pasture Systems and Watershed Management Research Unit, University Park, PA 16802, USA; 6University of Florida, Gainesville, FL 32608, USA; 7Agriculture and Agri-Food Canada, Lethbridge AB, Canada T1J 4B1; 8Innovation Center for U.S. Dairy, Rosemont, IL 60018, USA; 9Department of Animal Sciences, University of California, Davis, CA 95616, USA; 10DairyNZ, Hamilton 3240, New Zealand; 11Department of Animal Sciences, Wageningen University, 6700 AH Wageningen, The Netherlands (Received 28 February 2013; Accepted 15 April 2013) Although livestock production accounts for a sizeable share of global greenhouse gas emissions, numerous technical options have been identified to mitigate these emissions. In this review, a subset of these options, which have proven to be effective, are discussed.
  • Bromothymol Blue

    Bromothymol Blue

    Safety Data Sheet Bromothymol Blue 1. PRODUCT AND COMPANY IDENTIFICATION Product Name: Bromothymol Blue Synonyms/Generic Names: 4,4'-(3H-2,1-Benzoxathiol-3-ylidene)bis[2-bromo-3-methyl-6-(1- methylethyl)phenol]S,S-dioxide sodium salt SDS Number: 116.00 Product Use: For Educational Use Only Manufacturer: Columbus Chemical Industries, Inc. N4335 Temkin Rd. Columbus, WI. 53925 For More Information Contact: Ward's Science 5100 West Henrietta Rd. PO Box 92912-9012 Rochester, NY 14692 (800) 962-2660 (Monday-Friday 7:30-7:00 Eastern Time) In Case of Emergency Call: CHEMTREC - 800-424-9300 or 703-527-3887 (24 Hours/Day, 7 Days/Week) 2. HAZARDS IDENTIFICATION OSHA Hazards: No known OSHA hazards. Target Organs: None Signal Word: Warning Pictograms: None GHS Classification: Acute toxicity, Oral Category 5 GHS Label Elements, including precautionary statements: Hazard Statements: H303 May be harmful if swallowed. Precautionary Statements: P312 Call a POISON CENTER or doctor/physician if you feel unwell. Potential Health Effects Eyes May cause eye irritation. Inhalation May be harmful if inhaled. Causes respiratory tract irritation. Skin May be harmful if absorbed through skin. Causes skin irritation. Ingestion May be harmful if swallowed. Revised on 01/07/2013 Page 1 of 5 NFPA Ratings HMIS Ratings Health 1 Health 1 Flammability 1 Fire 1 Reactivity 0 Reactivity 0 Specific hazard Not Available Personal E 3. COMPOSITION/INFORMATION ON INGREDIENTS EINECS# / Molecular Component Weight % CAS # Formula ELINCS# Weight Bromothymol Blue 100 34722-90-2 N/A C27H27Br2NaO5S 646.36 g/mol 4. FIRST-AID MEASURES Eyes Rinse with plenty of water for at least 15 minutes and seek medical attention if necessary.
  • Thymol Decreases Apoptosis and Carotid Inflammation Induced by Hypercholesterolemia Through a Discount in Oxidative Stress

    Thymol Decreases Apoptosis and Carotid Inflammation Induced by Hypercholesterolemia Through a Discount in Oxidative Stress

    http://www.cjmb.org Open Access Original Article Crescent Journal of Medical and Biological Sciences Vol. 4, No. 4, October 2017, 186–193 eISSN 2148-9696 Thymol decreases apoptosis and carotid inflammation induced by hypercholesterolemia through a discount in oxidative stress Roshanak Bayatmakoo1, Nadereh Rashtchizadeh2*, Parichehreh Yaghmaei1, Mehdi Farhoudi3, Pouran Karimi3 Abstract Objective: Atherosclerosis sclerosis is a chronic inflammatory disease that can lead to cardiovascular and cerebrovascular disorders that are generally along with hypercholesterolemia and oxidative stress. Various surveys have shown that thymol is a polyphenolic compound with anti-inflammatory and antioxidant properties. This study aimed to investigate the anti- inflammatory and antiapoptotic effects of thymol on carotid tissue of hypercholesterolemic rats. Materials and Methods: Forty male Wistar rats were randomly divided into 4 groups with 10 members each (n = 10): a control group with a normal diet (ND), a group with a high-cholesterol (2%) diet (HD), a group with a high-cholesterol diet combined with thymol (24 mg/kg HD + T), and a group with a thymol diet (T). After preparing serum from peripheral blood of rats, lipid measurements were obtained, including total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and triglycerides (TG), by using a colorimetric method; the levels of oxidized LDL (OxLDL) were obtained through enzyme-linked immunosorbent assay (ELISA). The activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) antioxidant enzymes, as well as the concentrations of malondialdehyde (MDA) and serum total antioxidant capacity (TAC), were determined with the use of colorimetric methods. The protein expressions of Bcl2 and cleaved caspase 3 and the phosphorylation of p38 mitogen-activated protein kinase (MAPK) in rat carotid tissue were determined by an immunoblotting method.
  • Estrogen Pharmacology. I. the Influence of Estradiol and Estriol on Hepatic Disposal of Sulfobromophthalein (BSP) in Man

    Estrogen Pharmacology. I. the Influence of Estradiol and Estriol on Hepatic Disposal of Sulfobromophthalein (BSP) in Man

    Estrogen Pharmacology. I. The Influence of Estradiol and Estriol on Hepatic Disposal of Sulfobromophthalein (BSP) in Man Mark N. Mueller, Attallah Kappas J Clin Invest. 1964;43(10):1905-1914. https://doi.org/10.1172/JCI105064. Research Article Find the latest version: https://jci.me/105064/pdf Journal of Clinical Investigation Vol. 43, No. 10, 1964 Estrogen Pharmacology. I. The Influence of Estradiol and Estriol on Hepatic Disposal of Sulfobromophthalein (BSP) inMan* MARK N. MUELLER t AND ATTALLAH KAPPAS + WITH THE TECHNICAL ASSISTANCE OF EVELYN DAMGAARD (From the Department of Medicine and the Argonne Cancer Research Hospital,§ the University of Chicago, Chicago, Ill.) This report 1 describes the influence of natural biological action of natural estrogens in man, fur- estrogens on liver function, with special reference ther substantiate the role of the liver as a site of to sulfobromophthalein (BSP) excretion, in man. action of these hormones (5), and probably ac- Pharmacological amounts of the hormone estradiol count, in part, for the impairment of BSP dis- consistently induced alterations in BSP disposal posal that characterizes pregnancy (6) and the that were shown, through the techniques of neonatal period (7-10). Wheeler and associates (2, 3), to result from profound depression of the hepatic secretory Methods dye. Chro- transport maximum (Tm) for the Steroid solutions were prepared by dissolving crystal- matographic analysis of plasma BSP components line estradiol and estriol in a solvent vehicle containing revealed increased amounts of BSP conjugates 10% N,NDMA (N,N-dimethylacetamide) 3 in propylene during estrogen as compared with control pe- glycol. Estradiol was soluble in a concentration of 100 riods, implying a hormonal effect on cellular proc- mg per ml; estriol, in a concentration of 20 mg per ml.
  • Thinking Like a Chemist About Acids and Bases Part V UNIT 6 DAY 10

    Thinking Like a Chemist About Acids and Bases Part V UNIT 6 DAY 10

    Thinking Like a Chemist About Acids and Bases Part V UNIT 6 DAY 10 What are we going to learn today? Explore Acid – Base Titrations Explore the concept of protonation and pKa Explore Behavior of Polyprotic Acids Acid Base Titration Why do a titration? You have a solution with an unknown property Unknown Concentration? Unknown Ka (Kb)? Both Slowly neutralize the solution by adding a strong base (acid) monitor the pH with each addition What do these plots tell you? How is the plot on the right different? Work with neighbors on Titration Discovery Activity Poll: Clicker Question The initial concentration of the HBr is: A) 7 M B) 0.7 M C) 0.007 M D) 0.0007 M E) 3.5 M Poll: Clicker Question The pKa for acetic acid is: A) 1.7 x 10-5 B) 1.6 x 10-9 C) 4.76 D) 8.32 E) 3.43 Chemical Equilibrium pH indicator Chemical Equilibrium pH indicator Bromothymol Blue, pKa = 7.1 Protonated form Deprotonated form yellow blue Poll: Clicker Question Chemical Equilibrium pH indicator Bromothymol Blue Bromophenol Blue has a pKa of around 7. When it is protonated (HA form) it is yellow, when it is deprotonated (A- form) it is blue. What color would in be in a solution in which the pH was 9? A. blue B. yellow C. green CH302 Vanden Bout/LaBrake Spring 2012 Polyprotic Acid Poll: Clicker Question Polyprotic Acid -4 Ka1 = 7.4 x 10 -5 Ka2 = 1.7 x 10 -7 Ka3 = 4.0 x 10 At pH = 2 how many of the acidic protons will be on the molecule? A.
  • Bromothymol Blue Indicator, 0.04-1% Aqueous

    Bromothymol Blue Indicator, 0.04-1% Aqueous

    SDS Safety Data Sheet 1. IDENTIFICATION Product Identifier: Bromothymol Blue Indicator, 0.04-1% w/v Aqueous Product Code(s): B1013, B1020, B1021 Synonyms: BTB, Sodium Salt Solution; 3,3’-Dibromothymolsulfonphthalein, Sodium Salt Solution Recommended Use: For manufacturing, industrial, and laboratory use only. Use as a laboratory reagent. Uses Advised Against: Not for food, drug, or household use. Supplier: Rocky Mountain Reagents, Inc. 4621 Technology Drive, Golden, CO 80403 Phone: (303) 762-0800 Fax: (303) 762-1240 Emergency Phone Number: For health emergency, call poison control: (800) 222-1222. 2. HAZARDS IDENTIFICATION Hazard Classifications: This product is classified as not hazardous under OSHA's Hazard Communication Standard, 29 CFR 1910.1200 (HCS) and the United Nations’ Globally Harmonized System of Classification and Labeling of Chemicals (GHS). However, all chemicals handled and used in the workplace should be treated with caution. Signal Word: Not applicable. Hazard Statements: Not applicable. Pictograms: Not applicable. Precautionary Statements: Prevention: Not applicable. Response: Not applicable. Storage: Not applicable. Disposal: Not applicable. Hazards Not Otherwise Not applicable. Classified: Toxicity Statement: Not applicable. Product: Bromothymol Blue Indicator, 0.04-1% w/v Aqueous Revision Date: 05/19/2016 1/7 3. COMPOSITION AND INFORMATION ON INGREDIENTS Component Common Name / Synonyms CAS# Chemical Formula % by Weight Water Water 7732-18-5 H2O ≥ 99.0 Bromothymol Blue, 3,3’-Dibromothymolsulfonphthalein, 34722-90-2 NaC27H27O5SBr2 ≤ 1.00 Sodium Salt Sodium Salt Trade Secret Statement: Not applicable. 4. FIRST AID MEASURES First Aid Procedures: Inhalation: Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. Call a physician if symptoms occur.
  • Thymol, Menthol and Camphor from Indian Sources

    Thymol, Menthol and Camphor from Indian Sources

    THYMOL, MENTHOL AND CAMPHOR IN INDIA : CHOPRA & MUKHERJEE 361 ' sweetmeats, in pan supari' (betel leaf) mix- Articles tures, etc. The ajowan plant has, therefore, Original been grown to a greater or lesser extent all /over India. It is particularly abundant in Bengal, Central India (Indore) and Hyderabad THYMOL, MENTHOL AND CAMPHOrf (Deccan). 7,000 to 8,000 acres of land FROM INDIAN SOURCES Nearly are under cultivation each year in the Nizam's By R. N. CHOPRA, m.a., m.d. (Cantab.) Dominions alone and similar large areas are LIEUTENANT-COLONEL, I.M.S. also stated to be under cultivation in the and the United Provinces. i and Punjab Large quantities also find their way into India through B. m.b. MUKHERJEE, (Cal.) the inland routes from Afghanistan, Baluchistan Indigenous Drugs Enquiry, I. R. F. A., Series No. 35 and Persia. It can in fact be grown in any of the Indian Peninsula and the (From the Department of Pharmacology, School of part country Tropical Medicine, Calcutta) has possibilities of being a rich source of raw material for the of Indeed Thymol, menthol and camphor are well production thymol. this source has been the known in the materia medica of western already exploited by manufacturers as will be seen from the medicine as well as in that of the foreign indigenous of seeds from India between medicine in India. Thymol has been considered quantities exported 1911 and 1918 :? important on account of its powerful antiseptic, Value of germicidal and anthelmintic properties. One T otal the quantity seed of its chief uses in recent years has been in the in cwts.
  • Practical Problems Revised Grading Scheme

    Practical Problems Revised Grading Scheme

    Student Code AAA-1 Practical Problems Revised Grading Scheme "Bonding the World with Chemistry" 49th INTERNATIONAL CHEMISTRY OLYMPIAD Nakhon Pathom, THAILAND Practical problems (official English version), 49th IChO 2017, Thailand 1 Student Code AAA-1 General Instructions. General Information In 0.1 mol dm-3 HCl, indicators are in the acidic form (HIn) only. In 0.1 mol dm-3 NaOH, indicators are in the basic form (In-) only. There will be no mark for the answer in the dotted line box. NOTE: Students are suggested to check the spectrophotometer before use by measuring the absorbance values of the instrument check solution at two different wavelengths, i.e., 430 and 620 nm. Spectrophotometer No. ________ is used throughout the experiment. Record the absorbance values of the instrument check solution A (at 430 nm) A (at 620 nm) Measured value ________________ ________________ Guided value 0.220 – 0.260 0.450 – 0.510 In case that the measured values are within the guided values, students can proceed with further experiments. If not, students can ask for assistance. Part a Absorbance measurement of an acid-base indicator (methyl orange) in strong acid and strong base 1. Pipette 1.50 cm3 of 2.00 10-4 mol dm-3 methyl orange indicator solution into a 25.00- cm3 volumetric flask, add 2.5 cm3 of 1 mol dm-3 HCl into the flask and make up to the volume using distilled water. Record the absorbance at 470 and 520 nm. 2. Pipette 2.00 cm3 of 2.00 10-4 mol dm-3 methyl orange indicator solution into a 25.00- cm3 volumetric flask, add 2.5 cm3 of 1 mol dm-3 NaOH into the flask and make up to the volume using distilled water.
  • Investigation of Antifungal Mechanisms of Thymol in the Human Fungal Pathogen, Cryptococcus Neoformans

    Investigation of Antifungal Mechanisms of Thymol in the Human Fungal Pathogen, Cryptococcus Neoformans

    molecules Article Investigation of Antifungal Mechanisms of Thymol in the Human Fungal Pathogen, Cryptococcus neoformans Kwang-Woo Jung 1,*, Moon-Soo Chung 1, Hyoung-Woo Bai 1,2 , Byung-Yeoup Chung 1 and Sungbeom Lee 1,2,* 1 Radiation Research Division, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-si 56212, Jeollabuk-do, Korea; [email protected] (M.-S.C.); [email protected] (H.-W.B.); [email protected] (B.-Y.C.) 2 Department of Radiation Science and Technology, University of Science and Technology, Daejeon 34113, Yuseong-gu, Korea * Correspondence: [email protected] (K.-W.J.); [email protected] (S.L.) Abstract: Due to lifespan extension and changes in global climate, the increase in mycoses caused by primary and opportunistic fungal pathogens is now a global concern. Despite increasing attention, limited options are available for the treatment of systematic and invasive mycoses, owing to the evo- lutionary similarity between humans and fungi. Although plants produce a diversity of chemicals to protect themselves from pathogens, the molecular targets and modes of action of these plant-derived chemicals have not been well characterized. Using a reverse genetics approach, the present study re- vealed that thymol, a monoterpene alcohol from Thymus vulgaris L., (Lamiaceae), exhibits antifungal Cryptococcus neoformans activity against by regulating multiple signaling pathways including cal- cineurin, unfolded protein response, and HOG (high-osmolarity glycerol) MAPK (mitogen-activated protein kinase) pathways. Thymol treatment reduced the intracellular concentration of Ca2+ by Citation: Jung, K.-W.; Chung, M.-S.; Bai, H.-W.; Chung, B.-Y.; Lee, S.
  • Lab.10. Equilibrium. Determination of Dissociation Constant for a Weak Acid

    Lab.10. Equilibrium. Determination of Dissociation Constant for a Weak Acid

    Lab.8. Equilibrium. Determination of dissociation constant for a weak acid Key words: Equilibrium, equilibrium constant, dissociation constant, electrolytes, non electrolyte, weak and strong acids (bases), dissociation, titration, buffer solutions, calculation the pH of buffer solution, the Henderson-Hasselbalch equation, the unique properties of buffer solution (effect of dilution, effect of added acids and bases, buffer capacity). Literature J. A. Beran; Laboratory Manual for Principles of General Chemistry, pp. 281-346. M. Hein and S. Arena: Introduction to Chemistry, 13th ed. Wiley 2011; pp. J. Crowe. T. Bradshaw, P. Monk, Chemistry for the Biosciences. The essential concepts., Oxford University Press, 2006; Chapter 16, 482 - 512. J. Brady, N. Jespersen, A. Hysop, Chemistry, International Student Version, 7thed. Wiley, 2015, Chapter 14, 686 – 709. P. Monk, Physical chemistry, Wiley 2004, Chapters, 5, 6, 177 – 229 and 233- 276, Theoretical background The Swedish chemist Arrhenius suggested that solutions that conduct electricity, so called electrolytes, do so because they dissociate into charged species called ions. Compounds of this type: acids, bases and salts may be classified as strong electrolytes dissociate in solution almost completely into ions, weak electrolytes dissociate only to a small extent in solution (only 10% or less ions are present in solution). Acids are compounds that ionize to release hydrogen ions, H+, to their surroundings. Bases are compounds that accept hydrogen ions, The equation for the ionization of a weak acid may be expressed as: HA ↔ H+ + A- (1) However, this suggests that protons exist free in solution. The reality is that protons are solvated in solution, that is they go around attached to a solvent (water) molecules.
  • Bogen Universal Indicator Solution

    Bogen Universal Indicator Solution

    Material Safety Data Sheet Bogen Universal Indicator Solution Revised: 06/25/2012 Replaces: 04/25/2012 Printed: 08/31/2012 Section 1 - Product Description Product Name: Bogen Universal Indicator Solution Product Code(s): 848263, 848265, C70402, C70403 Size: 100 ml, 500 ml, 4 oz, 15 ml Chemical Name: See Section 3 CAS Number: See Section 3 Formula: See Section 3 Synonyms: None known Distributor: Carolina Biological Supply Company, 2700 York Road, Burlington, NC 27215 Chemical Information: 800-227-1150 (8am-5pm (ET) M-F) Chemtrec 800-424-9300 (Transportation Spill Response 24 hours) Section 2 - Hazard Identification Emergency Overview: WARNING - Flammable. Irritating to eyes and skin. Potential Health Effects: Eyes: May cause irritation. Skin: May cause irritation to skin. Ingestion: May cause gastrointestinal discomfort. Inhalation: May cause irritation to respiratory tract. Section 3 - Composition / Information on Ingredients Principal Hazardous Components: Hexane (Denaturant) (CAS# 110-54-3) 0.5%; Methyl red (CAS# 493-52-7) 0.0185%; Bromothymol blue (CAS# 76-59-5) 0.06%; Phenolphthalein (CAS# 77-09-8) 0.064%; Ethanol (CAS# 64-17-5) 47.5%; Methyl isobutyl ketone (Denaturant) (CAS# 108-10-1; Water (CAS# 7732-18-5) Balance TLV units: ACGIH-TLV (Ethanol) 1000 ppm (TWA) ACGIH-TLV (Methyl Isobutyl Ketone) 50 ppm (TWA); 75 ppm (STEL) ACGIH-TLV (Hexane) 50 ppm (TWA) PEL units: OSHA-PEL (Ethanol) 1000 ppm (TWA) OSHA-PEL (Methyl Isobutyl Ketone) 100 ppm (TWA); 75 ppm (STEL) OSHA-PEL (Hexane) 500 ppm (TWA) Section 4 - First Aid Measures Emergency and First Aid Procedures: Eyes - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
  • Bromothymol Blue Solution 0 04 (S25207A) Canadasds En 2019

    Bromothymol Blue Solution 0 04 (S25207A) Canadasds En 2019

    Page: 1/8 Safety Data Sheet according to OSHA HCS (29CFR 1910.1200) and WHMIS 2015 Regulations Revision: July 05, 2019 1 Identification · Product identifier · Trade name: Bromothymol Blue Solution, 0.04% · Product code: S25207A · Recommended use and restriction on use · Recommended use: Laboratory chemicals · Restrictions on use: No relevant information available. · Details of the supplier of the Safety Data Sheet · Manufacturer/Supplier: AquaPhoenix Scientific, Inc. 860 Gitts Run Road Hanover, PA 17331 Phone: (717)632-1291 Toll-Free: (866)632-1291 [email protected] · Distributor: Fisher Science Education 6771 Silver Crest Road Nazareth, PA 18064 (800) 955-1177 · Emergency telephone number: ChemTel Inc. (800)255-3924 (North America) +1 (813)248-0585 (International) 2 Hazard(s) identification · Classification of the substance or mixture The product is not classified as hazardous according to the Globally Harmonized System (GHS). · Label elements · GHS label elements Not regulated. · Hazard pictograms: None. · Signal word: None · Hazard statements: None. · Precautionary statements: None. · Other hazards There are no other hazards not otherwise classified that have been identified. 3 Composition/information on ingredients · Chemical characterization: Mixtures · Components: 7732-18-5 Water >90% 34722-90-2 Sodium α-(3-bromo-5-isopropyl-4-oxo-2-methyl-2,5-cyclohexadienylidene)-2-(3- 5-10% bromo-4-hydroxy-5-isopropyl-2-methylphenyl)toluenesulphonate · Additional information: For the listed ingredient(s), the identity and/or exact percentage(s) are being withheld as a trade secret. For the wording of the listed Hazard Statements, refer to section 16. (Cont'd. on page 2) 49.4.0 Page: 2/8 Safety Data Sheet according to OSHA HCS (29CFR 1910.1200) and WHMIS 2015 Regulations Revision: July 05, 2019 Trade name: Bromothymol Blue Solution, 0.04% (Cont'd.