NEW PANELS SPOTLIGHT

Bring Your Biomarkers to Life HUMAN Your comprehensive guide to multiplex and single protein detection.

Analyte Quarterly, Vol. 2 2017 MOUSE RAT • MILLIPLEX® map Assays

• SMC™ (Single Molecule Counting) Assays CELL SIGNALING • ELISA

• RIA

• GyroMark™ HT Assays

• Custom Assay Development INSTRUMENTS SINGLE PROTEIN CUSTOM The life science business of Merck operates as MilliporeSigma in the U.S. and Canada. For Research Use Only. PROTEIN DETECTION PLATFORMS Previously undetectable, nowquantified,onemoleculeatatime with accuracyandprecision orexceeds thatmeets ELISAperformance. we build into each panel to produce results you trust we buildintoeachpaneltoproduceresults Flexibility and sensitivity: ourplatformsfit your purpose andsensitivity: Flexibility protein detectionplatformforyour needs We're here toguideyou tochoosethebest Platforms FitforYour Purpose Our custom kits fortheGyrolab Our customkits as 1 μLofsample requiring aslittle GyroMark™ High-throughput,nanoliter-scale assays HTKits: intracellular biomarkers. Searchforyour atSigmaAldrich.com target . We have acombinedofferingof>1800ELISAs, EIAsandRIAsagainstnumeroussolublecirculating withourclassicassay your kits criticaltargets Quantify ELISAs: technology (developed by Singulex Combine atraditionalimmunoassay workflowwithultrasensitive SingleMoleculeCounting(SMC™) SMC™ assays forusewithSMCxPRO™ orErenna (e.g., MILLIPLEX andstability). detectability during ourvalidation process:cross-reactivity, dilutionlinearity, kitstability, andsamplebehavior In addition totheassay specificationslistedintheprotocol, we evaluate criteria otherperformance MILLIPLEX across awiderangeofresearch areas andspecies. • GoodPerformance MerckMillipore.com/immunoassays Gyrolab ELISA SMCxPRO™ orErenna Platforms Detection Protein Luminex ® ® workstation platform ® Fit forPurpose High precision Fully automated Most widelycited Plate reader compatibility High performance Ultrasensitivity Flexible platform Multiplex detection multiplex detection: rely on the quality mapmultiplexdetection:rely onthequality •• StrongPerformance ® system ® platformare fullyvalidated forreliable useintranslationalresearch, ® , Inc.) to quantify concentrationsdowntothefemtogram/mL, Inc.)toquantify level. Quantitative ••• SuperiorPerformance Yes Yes Yes Yes ®

assays offer the broadest selection of analytes map assays offerthebroadestselectionofanalytes Sensitivity pg/mL pg/mL pg/mL fg/mL 50–100 µL 5–100 µL ≤25µL Volume Sample < 5µL ® instruments: instruments: NotRecommended Dynamic Range ••• ••• ••• •• Capability Multiplex Recommended Support Custom Assay Assay Yes Yes Yes Yes NEW PANELS

PROTEIN DETECTION PLATFORMS For Research Use Only. Catalog No. Catalog No. MABMAG-83K MSHMAG-21K Coming Soon! Coming Soon! Coming Soon! Coming Soon! RMYOMAG-88K EZHMWAN-65K HCVD6MAG-67K | 1 MerckMillipore.com/immunoassays map Panels ® map listings: ® Mouse Human Species Species Human Human Human Rat Human Multi-Species Human kits, so you don’t have to do it yourself. have don’t so you map kits, ® and Single Protein Detection Kits Detection Protein Single and map ® map Panels map Panels ® ® Available for any number of kits for any Available number of analytes within a qualifying kit for any Available included beads are all of the requested quality assurance tested to ensure vials are Premixed days business Allow up to 10 additional Autoimmune Autoantibody Amyloid β 1-40 and Amyloid β 1-42, 2-plex β 1-40 and Amyloid Amyloid Description Description Bcl-2 Family Apoptosis Panels 1 and 2 Panels Apoptosis Bcl-2 Family Cardiovascular Disease Panel 6 Disease Panel Cardiovascular MILLIPLEX MILLIPLEX Immuno-Oncology Checkpoint Protein Panel Myokine Neuroscience Panel 2 Neuroscience Panel Hormone Panel ELISAs Adiponectin High Molecular Weight Custom Premix Kit Option for MILLIPLEX Custom Premix mixing for selected MILLIPLEX now offer bead We Coming Soon NEW! MILLIPLEX NEW! For qualifying kits, look for this symbol in the MILLIPLEX look for this symbol qualifying kits, For • • • • SPOTLIGHT

For Research Use Only. NEW PRODUCT SPOTLIGHT Erenna These results demonstratethat theSMCxPRO™ instrument gives similarsampleresults tothosefromthe R Figure 1.For measured, theanalytes themean interpolatedconcentration of eachsample(R ontheSMCxPRO™The sameplatewasanalyzedfirst instrument,andthenontheErenna Shown beloware results forindividual andpooledplasmasamplesfromfourSMC™immunoassay kits. SMCxPRO™ vs. Erenna two instruments.two Human cTNI(#03-0092-00)R 2 |MerckMillipore.com/smcxpro • • • • • This compactbenchtopsolutionoffers: technology you canrely ontodetectyour target. lowlevelsand quantifying ofproteins, you need When thesuccessofyour research requires finding assaysensitivity needs. offers you alowercostsolutiontomeet your high The newSMCxPRO™ Immunoassay System Discovery DependsonDetection No.(Cat. 95-0100-00) SMCxPRO™ Platform New ProductSpotlight • 2 =0.9976, andHuman IL-23 (#03-0112-00) R Access too R Compact: 14”WX16”H17.5”D Bead- orplate-basedassa Rapid analy High sensitivit

pg/mL pg/mL eady-made tofullycustomizedkits 0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 0.00 0.10 0.20 0.30 0.40 0.50 0.60 0.70 0.80 ® instrument and researchers can confidently move instrumentandresearchers canconfidently fromoneinstrument totheother. Normal HumanPlasma:MeanInterpolatedcTnI,pg/mL Normal HumanPlasma:MeanInterpolatedIL-4,pg/mL ver 600antibodypairs sis y (femtogram/mL) LLoQ =0.69pg/mL LLoQ =0.04pg/mL SMCxPRO SMCxPRO Sample ID Sample ID y format TM TM Erenna Erenna ® InstrumentSampleValue Comparisons ® ® 2 2 =0.9413. =0.9944, Human IL-6 (#03-0089-01) R MerckMillipore.com/smcxpro Learn more at abundant proteinsinlessthananhour. on theSMCxPRO™ low- platformandquantify For reliable andaffordableproteindetection,count pg/mL pg/mL 0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 0.00 0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 Normal HumanPlasma:MeanInterpolatedIL-23,pg/mL Normal HumanPlasma:MeanInterpolatedIL-6,pg/mL

2 =0.9972, Human IL-4 (#03-0052-00) LLoQ =0.10pg/mL LLoQ =0.08pg/mL SMCxPRO SMCxPRO 2 ) was nearly identical between the ) wasnearlyidentical between Sample ID Sample ID TM TM Erenna Erenna ® ® ® instrument. instrument.

SPOTLIGHT

NEW PRODUCT SPOTLIGHT For Research Use Only. page, ters, and e types. A e types. l on a single l on a single ✔ ✔ | 3 MerckMillipore.com/elisa_ria species and sampl species and o list them al o list them e t ylation events cardiovascular disease to metabolic disease and cardiovascular performance parame , performance iew protocols Competitive ELISA Competitive to detect lysates use on cell For phosphor is impossibl v and – – t EIA – Cell-Based Phosphorylation ELISA – • • ou can also rom inflammation rom inflammation Metabolic Cardiovascular Angiogenesis Signaling . Y illipore.com e and validation LISAs and EIAs, covering a diverse range of diverse a covering EIAs, LISAs and km addressed, f addressed, 0 E

✔ ✔ ✔ ✔ ✔ ✔✔ ✔ ✔✔ ✔ ✔✔ ✔ ✔ ✔ ✔ ✔ ✔ ✔ ✔ Cytokines arget at merc ant olio of > 1,80 f search areas are search areas Requires custom manufactur custom Requires apply Upcharge may Some may be designated “lysate” to be designated “lysate” Some may change from serum, plasma accommodate the or supernat Standard off-the-shelf assay – – – – Custom Sandwich ELISA Custom Sandwich – – – Sandwich ELISA – • ELISA Designations variety of re variety of ELISAs, i a large number research. With such veterinary your t so search . Certificate of Analysis • We offer a port offer We ELISAs SPOTLIGHT

For Research Use Only. NEW PRODUCT SPOTLIGHT 4 |MerckMillipore.com/elisa_ria adiponectin ratioof0.53(Figure 2)whichcorrelates wellwiththeexpectedvalue. Our HumanHMWAdiponectinELISAand(Total) AdiponectinELISA(EZHADP-61K) give aHMW/Total kit issufficienttomeasure 37samplesinduplicate. step. Samplesfortheassay orculture may media.One extracts include humanserum,plasma,adipocyte EZHMWAN-65K isasimplesandwichELISAwhichfirst requires a2.5hoursample pre-treatment/digestion This kitreplaces ourdiscontinuedHMWAdiponectinELISA withanimproved, more streamlined workflow. isoforms, givingaHMW/Total ratioof~0.5. glucosehomeostasis. of maintaining TheHMWadiponectinisoformcomprises~50%ofallthree adiponectin and isassociatedwithalowerincidenceofdiabetes. Itisbelieved tobethemostbiologicallyactive interms Synthesized by adipocytes, circulatingHMWadiponectinmediatesinsulinsensitizationinperipheraltissues Weight AdiponectinELISAhasbeenvalidated ofourEZ-line tothehighstandards ofELISAassays. Introducing thenewestaddition TheHumanHigh Molecular ELISAportfolio. toourextensive No.(Cat. EZHMWAN-65K) Human HighMolecularWeight (HMW)AdiponectinELISA 1. Reference previous kit,EZHMWA-64K. results correlate withpublished results fromour our newHumanHMWAdiponectinELISA.These vs. Type IIdiabetespatients. All were assayed using Adiponectin fromsamplesnormal/non-diabetic In fromBIORECLAMATION,adiponectin ratiois0.53.Allsamplesobtained LLC, Westbury, NY. Figure 2.Assay ofhumanserum/plasmasamples(n=32)inEZHMWAN-65K vs. EZHADP-61K. Theresulting HMW/Total 2144-2151 (2007). etal.,ClinicalChemistry,Sinha, M.K., 53:12, , we show plasma concentrations of HMW Figure 3,weshowplasmaconcentrationsofHMW

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NEW PRODUCT SPOTLIGHT For Research Use Only. 200™ ® | 5 MerckMillipore.com/milliplex AM MC ucMGP • • From the same assay as described in Figure 3, these as described in Figure assay the same 5. From Figure four analytes show somewhat higher ng/mL concentrations. Statistical significance was found between the normal and CVD samples for only ucMGP (p=0.0018). Human normal and cardiovascular disease positive 4. Human normal and cardiovascular Figure from purchased (CVD) were serum/plasma samples (n=18) using the and assayed NY, Westbury, LLC, BIORECLAMATION 6 with a Luminex Disease Panel Human Cardiovascular ng/ as average instrument. Data expressed for each analyte are found to have were mL +/- SEM. The four analytes shown here for both normalthe lowest ng/mL concentration in serum/plasma Statistical between(non-CVD) significance CVD and samples. normal and CVD samples was found in the analytes Endostatin (p=0.0002), pGRN (p=0.03) and MCAM (p=0.00001). map panels to measure the latest biomarkers the latest to measure map panels

®

DPP4

LRG1 MCAM* G1 pGRN LR

• • ucMGP* pGRN* map Human Cardiovascular Disease Panel 6 Cardiovascular Human map Panel Disease

® CD14

Control Avg (n=18)Control CVD (n=18) Avg Control Avg (n=18)Control CVD Avg (n=18) Endostatin* atin 5 0

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ng/mL DPP4 Endost ng/mL CD14 Introducing the 6th in our series of MILLIPLEX the 6th in our Introducing MILLIPLEX HCVD6MAG-67K) (Cat. No. of cardiovascular disease in human serum, plasma or cell culture samples. plasma or cell culture human serum, disease in of cardiovascular the following analytes or all of this 7-plex panel: from Choose any • • • HUMAN

For Research Use Only. MILLIPLEX® map IMMUNOLOGY 6 |MerckMillipore.com/milliplex Panel I Human Cytokine/Chemokine Immunology HUMAN (Cat. No.(Cat. HCYP3MAG-63K) Panel III Human Cytokine/Chemokine Panel II Human Cytokine/Chemokine 41 41 38 38 30 30 29 29 23 23 Lymphotactin /XCL1 LIX /CXCL6 /GCP-2 I-TAC /CXCL11 IL-29 /IFNλ1 IL-11 HCC-1 /CCL14▲ IL-23 IL-21 IL-20 IL-16 I-309/CCL1 Eotaxin-3/CCL26 Eotaxin-2/CCL24/ ENA-78/CXCL5 CTACK/CCL27 BCA-1/CXCL13 6Ckine/CCL21/ IL-8 /CXCL8 ♦ IL-7 IL-6 IL-5 IL-4 IL-3 IL-2 IL-1Ra IL-1β IL-1α IFNγ IFNα2 GRO GM-CSF G-CSF Fractalkine /CX3CL1 Flt3 Ligand FGF-2 /FGF-basic /CCL11♦ Eotaxin EGF sCD40L

MPIF-2 Exodus-2 No. (BulkCat. HCYTMAG60PMX41BK) No. (Cat. HCYTMAG-60K-PX41) No. (BulkCat. HCYTMAG60PMX38BK) No. (Cat. HCYTMAG-60K-PX38) No.(Bulk Cat. HCYTMAG60PMX30BK) No. (Cat. HCYTMAG-60K-PX30) (Bulk Cat. No.(Bulk Cat. HCP2MAG62KPX23BK) No.(Cat. HCP2MAG-62K-PX23) No.(Bulk Cat. HCYTMAG60PMX29BK) No.(Cat. HCYTMAG-60K-PX29) (Cat. No.(Cat. HCYP2MAG-62K) No.(Cat. HCYTOMAG-60K) ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ TNFβ /Lymphotoxin-α TNFα TGFα RANTES /CCL5♦ PDGF-AB /BB VEGF-A NAP-2 /CXCL7 ▲ MIP-3β /CCL19 MIP-3α /CCL20 MIG /CXCL9 M-CSF TSLP TRAIL/TNFSF10 TPO TARC/CCL17 SDF-1/CXCL12 SCF MIP-1δ/MIP-5/CCL15 MCP-4/CCL13 MCP-2/CCL8 LIF IL-33/NF-HEV IL-28A/IFNλ2 PDGF-AA MIP-1β /CCL4♦ MIP-1α /CCL3♦ MDC /CCL22 MCP-3 /CCL7 MCP-1 /CCL2♦ IP-10 /CXCL10 ♦ IL-17A ♦ /CTLA8 IL-15 IL-13 IL-12 (p70) IL-12 (p40) IL-10 IL-9 (LTA) (mature) ♦ ♦ ♦ ♦ ♦ ♦ ▲ ♦ ♦ ♦ ▲ ▲ ♦ T Cell Human HighSensitivity Panel IV Human Cytokine/Chemokine (Cat. No.(Cat. HSCRMAG-32K) Receptor Human SolubleCytokine No.(Cat. HSTC384-28K) TCell Sensitivity 384-well HumanHigh 21 21 21 21 21 13 13 14 14 21 IL-10 IL-8 /CXCL8 ♦ IL-7 IL-6 IL-5 IL-4 IL-2 IL-1β IFNγ GM-CSF Fractalkine /CX3CL1 IL-24 IL-19 IL-14 /α-Taxilin IFNβ HMGB1 ● HCC-4 /CCL16 CXCL16 CCL28 BRAK /CXCL14 BAFF /Blys APRIL /TNFSF13 sIL-4R sIL-2Rα sIL-1RII sIL-1RI sgp130 sEGFR sCD30 IL-10 IL-8 /CXCL8 IL-7 IL-6 IL-5 IL-4 IL-2 IL-1β IFNγ GM-CSF Fractalkine /CX3CL1

(Bulk Cat. No. (BulkCat. HSTCMAG28PMX21BK) No. (Cat. HSTCMAG28SPMX21)

(Bulk Cat. No.(Bulk Cat. HSTCMAG28PMX13BK) No.(Cat. HSTCMAG28SPMX13) No.(Cat. HCP4MG64KPX21BK) No.(Cat. HCY4MG-64K-PX21) (Bulk Cat. No.(Bulk Cat. HSCRMAG32PMX14BK) No.(Cat. HSCRMAG32KPX14) No.(Bulk Cat. HSTCMAG384PX21BK) No.(Cat HSTCMAG384-PX21) (Cat. No.(Cat. HSTCMAG-28SK) No.(Cat. HCYP4MAG-64K) ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ TNFα MIP-3α /CCL20 MIP-1β /CCL4 MIP-1α /CCL3 I-TAC /CXCL11 IL-23 IL-21 IL-17A /CTLA8 IL-13 IL-12 (p70) YKL40/CHI3L1 MPIF /CCL23 MIP-4 /PARC /CCL18 IL-38 /IL-1F10 IL-37 /IL-1F7 IL-36β /IL-1F8 IL-35 IL-34 IL-32α IL-28B /IFNλ3 sVEGFR3 /sFlt-4 sVEGFR2 /sKDRsFlk-1 sVEGFR1 /sFlt-1 sTNF RII sTNF RI sRAGE sIL-6R TNFα MIP-3α /CCL20 MIP-1β /CCL4 MIP-1α /CCL3 I-TAC /CXCL11 IL-23 IL-21 IL-17A /CTLA8 IL-13 IL-12 (p70) ♦ ♦ ♦ ♦ ♦ Human CD8+TCell No.(Cat. HTH17MAG-14K) Human Th17 (Cat. No.(Cat. HMMP2MAG-55K) Human MMPPanel 2 No.(Cat. HMMP1MAG-55K) Human MMPPanel 1 No.(Cat. HCMP2MAG-19K) PanelHuman Complement 2 No.(Cat. HCMP1MAG-19K) PanelHuman Complement 1 17 17 25 25 IL-2 IFNγ Granzyme B Granzyme A GM-CSF sFasL sFAS /TNFRSF6 sCD137/4-1BB/ IL-17A /CTLA8 IL-15 IL-13 IL-12 (p70) IL-10 IL-9 IL-6 IL-5 IL-4 IL-2 IL-1β IFNγ GM-CSF MMP-7 MMP-2 MMP-1 MMP-12 MMP-3 C4 C3b /iC3b C3 C1q C5 C4b C2 Adipsin/Factor D

TNFRSF9

(Bulk Cat. No.(Bulk Cat. HCD8MAG15KPX17BK) No.(Cat. HCD8MAG15K17PMX) No.(Bulk Cat. HT17MAG14PMX25BK) No.(Cat. HT17MG-14K-PX25) (Cat. No.(Cat. HCD8MAG-15K) TNFα Perforin MIP-1β /CCL4 MIP-1α /CCL3 IL-13 IL-10 IL-6 IL-5 IL-4 TNFβ/Lymphotoxin-α TNFα MIP-3α /CCL20 IL-33 /NF-HEV IL-31 IL-28A /IFNλ2 IL-27 IL-23 IL-22 IL-21 IL-17F IL-17E /IL-25 MMP-10 MMP-9 MMP-13 Factor P/Properdin Factor H Factor B Mannose-binding Factor I C9 C5a (LTA) (mature) Lectin (MBL) HUMAN

MILLIPLEX® map IMMUNOLOGY / METABOLISM/ENDOCRINOLOGY For Research Use Only. ◗ Polypeptide (PP) Polypeptide ▼ FGF-19 FGF-21 FGF-23 HGF IGFBP5 IGFBP6 IGFBP7 IGF-2 Insulin Leptin IL-6 Insulin Leptin MCP-1/CCL2 Pancreatic (total)PYY TNFα IL-15 Irisin LIF Myostatin/GDF8 Oncostatin-M (OSM) Osteocrin/Musclin Osteonectin/SPARC ■ ◗ ◗ ◗ ◗ | 7 MerckMillipore.com/milliplex Serum/Plasma only samples only Tissue Culture format assay Competitive Available for custom premix Available (Bulk Cat. No. HDIAB34KPMX5BK) (Bulk Cat. No.

(Cat.No. HDIAB-34K-PMX5) ● ★ ^ α-Fetoprotein (AFP) α-Fetoprotein ANGPTL3 ANGPTL4 ANGPTL6 FABP1 IGFBP1 IGFBP2 IGFBP3 IGFBP4 IGF-1 C-Peptide (active) GLP-1 Glucagon Amylin † ◗ (active) Amylin (total) † ◗ C-Peptide (active) Ghrelin GIP (total) GLP-1 † ◗ (active) GLP-1 (total) † ◗ Glucagon Apelin BDNF Erythropoietin (EPO) FABP3 FGF21 Fractalkine/CX3CL1 FSTL1 IL-6 NON-CONFIGURABLE KIT NON-CONFIGURABLE 5 5 Human Liver Protein Human Liver HLPPMAG-57K) (Cat. No. Human IGF Binding Protein HIGFBMAG-53K) (Cat. No. Human IGF HIGFMAG-52K) (Cat. No. Diabetes Human Human Metabolic Hormone HMHEMAG-34K) (Cat. No. Human Myokine HMYOMAG-56K) (Cat. No. Leptin MCP-1/CCL2 NGF TNFα PAI-1 (total) PAI-1 Resistin MCP-1/CCL2 NGF (total) PAI-1 Resistin TNFα Involucrin Keratin-1,10 Keratin-6 IgG3 IgG4 IgM These analytes cannot be plexed with otherThese analytes cannot be plexed analytes in this panel in serum/plasma a protease inhibitor during Requires sample collection sample extraction Requires (Cat. No. HGAMMAG-301K) (Cat. No. ▲  ◗ ■ Albumin (HSA)

HGF IL-1β IL-6 IL-8/CXCL8 Insulin Adiponectin HGF IL-1β IL-6 IL-8/CXCL8 Leptin Adiponectin D Adipsin/Factor NGAL/Lipocalin-2 IgA IgG1 IgG2 IgE Cortisol ^ Fibronectin Human Serum (Cat. No. HADK2MAG-61K) (Cat. No. Human Adipocyte (Cell samples) culture HADCYMAG-61K) (Cat. No. Metabolism/ Endocrinology 1 Human Adipokine Panel (Serum/Plasma samples) HADK1MAG-61K) (Cat. No. 2 Human Adipokine Panel (Serum/Plasma samples) Human Immunoglobulin Human Immunoglobulin Isotyping – Singleplex Human IgE HGAMMAG-303E) (Cat. No. Skin Human extracts(Skin only) SKINMAG-50K) (Cat.No.

map kits ▼ ® (TSP-1) Resistin Thrombospondin-1 MIF (total) PAI-1 sVCAM-1 / CCL3 MIP-1α / CCL4 MIP-1β MMP-8 TGFβ3 TIMP-2 TIMP-3 TIMP-4 Analytes which cannot be plexed together:Analytes which cannot be plexed – Active and total – Active and total – Free listed in Cat. No. Available Premix panel only Premix

▼ † Legend key for MILLIPLEX key Legend ♦ (ELA2) Lactotransferrin (LTF) Lactotransferrin Neutrophil Elastase-2 NGAL/Lipocalin-2 Granzyme B HSP70 IL-1α / CXCL8 IL-8 / TNFRSF6 sFAS sFasL sICAM-1 TGFβ1 TGFβ2 TGFβ1 TIMP-1 TIMP-2 TIMP-1 NON-CONFIGURABLE KIT Human Sepsis Panel 3 Human Sepsis Panel HSP3MAG-63K) (Cat. No. Human Sepsis Panel 2 Human Sepsis Panel HSP2MAG-63K) (Cat. No. Human Sepsis Panel 1 Human Sepsis Panel HSP1MAG-63K) (Cat. No. Immune Response (Cat. No. TGFBMAG-64K-03) (Cat. No. Multi-Species TGFβ – 3 Plex TGFβ Multi-Species (Cat. No. TGFBMAG-64K-01) (Cat. No. TGFBMAG-64K-01BK) (Bulk Cat. No. Multi-Species TGFβ – TGFβ Multi-Species Singleplex (Cell culture samples) (Cell culture HTMP2MAG-54K) (Cat. No. Human TIMP Panel 2 Panel Human TIMP Human TIMP Panel 1 Panel TIMP Human samples) (Serum/Plasma HTMP1MAG-54K) (Cat.No. HUMAN

For Research Use Only. MILLIPLEX® map BONE / CVD / CANCER / METABOLISM / ENDOCRINOLOGY 8 |MerckMillipore.com/milliplex (Cat. No.(Cat. STTHMAG-21K) Hormone Multi-Species Steroid/Thyroid No.(Cat. HPTP1MAG-66K) Panel 1 Human Pituitary (Cat. No.(Cat. HCVD4MAG-67K) PanelHuman CVD 4 No.(Cat. HCVD3MAG-67K) (Acute Phase) PanelHuman CVD 3 No.(Cat. HCVD2MAG-67K) PanelHuman CVD 2 No.(Cat. HCVD1MAG-67K) PanelHuman CVD 1 Cardiovascular No.(Cat. MSHMAG-21K) Panel Multi-Species Hormone Pentraxin-3 (PTX3) Follistatin (FST) dPAPP-A sCD31/sPECAM-1 Fibrinogen Fetuin A CRP Adipsin/Factor D α-2-Macroglobulin α-1-Acid Glycoprotein Myeloperoxidase sICAM-1 GDF-15 D-dimer ADAMTS13 LIGHT FABP4 FABP3 Endocan-1 (ESM-1) CXCL16 CK-MB BNP Progesterone Estradiol Cortisol Progesterone Estradiol Cortisol CNTF Agouti-Related ACTH (AGP) (MPO) Protein (AgRP) ■

^ ■

^ Troponin T(TnT) Tissue Factor (TF) Thrombomodulin sE-Selectin von WillebrandFactor Serum Amyloid P Platelet Factor 4 sL-Selectin Haptoglobin sVCAM-1 Serum Amyloid A sP-Selectin NGAL/Lipocalin-2 Myoglobin Troponin I(TnI) Growth Placental (OSM) Oncostatin-M NT proBNP LIX/CXCL6/GCP-2 T4 T3 Testosterone T4 T3 TSH LH GH FSH (vWF) (SAP) (PF4)/CXCL4 Factor (PLGF) NEW Bone No.(Cat. APOMAG-62K) Human Apolipoprotein No.(Cat. HCVD6MAG-67K) PanelHuman CVD 6 No.(Cat. HCVD5MAG-67K) PanelHuman CVD 5 (Cat. No.(Cat. HCCBP1MAG-58K) Biomarker Panel 1 Human CirculatingCancer Cancer No.(Cat. HRNKLMAG-51K-01) Human RANKL–Singleplex No.(Cat. HBNMAG-51K) Human Bone Apo B Apo AII Apo AI pGRN Endostatin DPP4 sCD14 LDLR IGF1R HSP60 Chromogranin-A sCD163 13(CDH13) Cadherin ADAM15 IL-6 HGF HE4 HCGβ FGF-2/FGF-basic sFasL sFAS/TNFRSF6 CYFRA21-1 CEA CA19-9 CA15-3 CA125 α-Fetoprotein (AFP) RANKL Leptin Insulin IL-6 IL-1β FGF-23 DKK1 ACTH (CHGA/CGA) Apo E Apo CIII Apo CII ucMGP MCAM LRG1 Syndecan 4(SYND4) sST2/IL1RL1 PTGDS PLA2G7 Notch1 MR-ProADM Leptin Receptor (LEPR) VEGF-A TRAIL/TNFSF10 TNFα TGFα SCF PSA (total)† PSA (free)† Prolactin Osteopontin (OPN) MIF Leptin IL-8/CXCL8 TNFα Sclerostin (SOST) PTH Osteoprotegerin (OPG) Osteopontin (OPN) Osteocalcin (OC) NEW (Cat. No.(Cat. HCCB4MAG-58K) Biomarker Panel 4 Human CirculatingCancer No.(Cat. HCCBP3MAG-58K) Biomarker Panel 3 Human CirculatingCancer No.(Cat. HCCBP2MAG-58K) Biomarker Panel 2 Human CirculatingCancer (Cat. No.(Cat. HAGP1MAG-12K) Factor Panel 1 Human Angiogenesis/Growth No.(Cat. HCMBMAG-22K) Biomarker Panel 1 Human Cancer/Metastasis Hepsin EpCAM CD44 AnhydraseCarbonic ALDH1A1 IGFBP3 Galectin 3 Fibroblast Activation Ferritin D Cathepsin Complement Factor Antithrombin III G-CSF Follistatin (FST) FGF-2/FGF-basic FGF-1/FGF-acidic Endothelin-1 Endoglin EGF BMP-9 Angiopoietin-2 Osteonectin/SPARC Neuron-specific GDF-15 DKK1 9 (CA9) Protein (FAP) H (CFH) Enolase (NSE) Transglutaminase 2 NCAML1/L1CAM/ Midkine Mesothelin Kallikrein-6 Sex HormoneBinding Myeloperoxidase Melanoma Inhibitory Vitamin DBinding Vitamin Matrix Extracellular VEGF-D VEGF-C VEGF-A Growth Placental Leptin IL-8/CXCL8 HGF HB-EGF YKL40/1CHI3L1 TWEAK TRAP5 Periostin Osteoprotegerin (TGM2) CD171 Globulin (SHBG) (MPO) (MIA) Activity Protein Protein 1(ECM1) Factor (PLGF) (OPG) HUMAN

MILLIPLEX® map CANCER / NEUROSCIENCE / TOXICITY For Research Use Only. Osteoactivin TIMP-1 Uromodulin C Cystatin RBP4 MDH1 SDH EGF NGAL/Lipocalin-2 Osteopontin (OPN) Uromodulin Osteopontin (OPN) PTH Renin | 9 MerckMillipore.com/milliplex Serum/Plasma only samples only Tissue Culture format assay Competitive Available for custom premix Available

● ★ ^ α-1-Microglobulin Collagen IV NGAL/Lipocalin-2 β-2-Microglobulin Clusterin 5'NT/CD73 ARG1 GSTα α-1-Microglobulin Albumin Clusterin C Cystatin β-2-Microglobulin RBP4 EGF FABP1 IP-10/CXCL10 KIM-1 Human Kidney Injury Panel 6 InjuryHuman Kidney Panel (Serum/Plasma samples) HKI6MAG-99K) (Cat. No. InjuryHuman Liver HLINJMAG-75K) (Cat. No. Human Kidney Injury Panel 2 2 Injury Kidney Human Panel samples) (Urine HKI2MAG-99K) (Cat.No. 3 InjuryHuman Kidney Panel (Urine samples) HKI3MAG-99K) (Cat. No. 4 InjuryHuman Kidney Panel (Serum/Plasma samples) HKI4MAG-99K) (Cat. No. 5 InjuryHuman Kidney Panel (Serum/Plasma samples) HKI5MAG-99K) (Cat. No. ^

■ ^

■ Dismutase 1 (sSOD1) Dismutase 2 (sSOD2) Melatonin KIM-1 Osteoactivin Renin TFF-3 TIMP-1 Osteopontin (OPN) Soluble Superoxide Soluble Superoxide A Orexin Oxytocin Substance P PAI-1 (total) PAI-1 PDGF-AA PDGF-AB/BB RANTES/CCL5 sVCAM-1 sRAGE S100B These analytes cannot be plexed with otherThese analytes cannot be plexed analytes in this panel in serum/plasma a protease inhibitor during Requires sample collection sample extraction Requires ▲  ◗ ■ (AGT) (MPO) Cortisol Calbindin Collagen IV FABP1 GSTα IP-10/CXCL10 Angiotensinogen Contactin-1 A Fetuin Kallikrein-6 α-MSH β-Endorphin Neurotensin Amyloid beta 1-40 Amyloid beta 1-42 Amyloid GDNF BDNF CathepsinD sICAM-1 Myeloperoxidase sNCAM Human Circadian Stress HNCSMAG-35K) (Cat. No. Toxicity 1 InjuryHuman Kidney Panel (Urine samples) HKI1MAG-99K) (Cat. No. Human Neurological Human Neurological 3 Disorders Panel HND3MAG-39K) (Cat. No. Human Neuropeptide HNPMAG-35K) (Cat. No. Human Neurodegenerative Human Neurodegenerative 4 Disease Panel (CSF samples) HNDG4MAG-36K) (Cat. No. Human Neurodegenerative Neurodegenerative Human 3 Panel Disease HNDG3MAG-36K) (Cat.No.

map kits ® (SAP) (TGM2) (TSP-2) MIP-4/PARC/CCL18 PEDF P Serum Amyloid PARK5/UCHL1 PARK7/DJ1 2 Transglutaminase Apo E Complement C3 H Complement Factor pTau (Thr181) pTau (total) Tau sIL-6Rα (sNRP-1) sNeuropilin-1 Osteopontin (OPN) PDGF-AB/BB C (TN-C) Tenascin Thrombospondin-2 sTIE-2 suPAR sVEGFR1/sFlt-1 sVEGFR2/sKDR/sFlk-1 sVEGFR3/sFlt-4 Analytes which cannot be plexed together:Analytes which cannot be plexed – Active and total – Active and total – Free listed in Cat. No. Available Premix panel only Premix

▼ † Legend key for MILLIPLEX key Legend ♦ Enolase (NSE) Protein (GFAP) sErbB2 sErbB3 Factor Receptor Receptor Factor (SCFR) α-1-Antitrypsin (A1AT) α-1-Antitrypsin C4 CRP α-2-Macroglobulin Apo AI Apo CIII Neuron-specific α-Synuclein Glial Fibrillary Acidic Amyloid beta 1-40 Amyloid beta 1-42 Amyloid sE-Selectin sEGFR/sHER1/sErbB1 sHER2/sEGFR2/ sHER3/sEGFR3/ sHGFR/sc-Met Angiostatin/Kringle sAXL sCD31/sPECAM-1 Cell sc-Kit/sStem (Cat. No. HNDG2MAG-36K) (Cat. No. Human Neurodegenerative Human Neurodegenerative 2 Disease Panel (Cat. No. HNDG1MAG-36K) (Cat. No. Human Neurodegenerative Human Neurodegenerative 1 Disease Panel Human Neuroscience Panel 1 Human Neuroscience Panel (CSF samples) HNS1MAG-95K) (Cat. No. Human Amyloid Beta and Tau Beta and Tau Human Amyloid (CSF samples) HNABTMAG-68K) (Cat. No. Neuroscience Human Angiogenesis Panel 2 Panel Angiogenesis Human HANG2MAG-12K) (Cat. No. PRIMATE

For Research Use Only. MILLIPLEX® map IMMUNOLOGY / METABOLISM / ENDOCRINOLOGY 10 |MerckMillipore.com/milliplex Cytokine/Chemokine Panel 1 Cytokine/Chemokine Non-Human Primate Immunology PRIMATE (Cat. No.(Cat. TGFBMAG-64K-03) Multi-Species TGFβ –3Plex No.(Bulk Cat. TGFBMAG-64K-01BK) No.(Cat. TGFBMAG-64K-01) Singleplex Multi-Species TGFβ – Panel 2 Cytokine/Chemokine Non-Human Primate 25 25 24 24 23 23 NON-CONFIGURABLE KIT TGFβ2 TGFβ1 TGFβ1 IL-17A/CTLA8 IL-16 IL-6 IL-4 IL-2 IL-1α Granzyme B Granzyme A Fractalkine/CX3CL1 FGF-2/FGF-basic sFasL Eotaxin/CCL11 sCD137/4-1BB/ IL-10 IL-8/CXCL8 IL-6 IL-5 IL-4 IL-2 IL-1Ra IL-1β IFNγ GM-CSF G-CSF sCD40L

TNFRSF9

(Bulk Cat. No.(Bulk Cat. PRCY2MG40PMX25BK) No.(Cat. PRCYTMAG40K-PX25) No.(Bulk Cat. PRCY2MG40PMX24BK) No.(Cat. PRCYTMAG40K-PX24) No.(Bulk Cat. PRCYMAG40PMX23BK) No.(Cat. PCYTMG-40K-PX23) (Cat. No.(Cat. PRCYT2MAG40K) No.(Cat. PRCYTOMAG-40K) TGFβ3 TNFβ/Lymphotoxin-α RANTES/CCL5 ▲ Perforin MIP-3α/CCL20 IP-10/CXCL10 IL-33/NF-HEV IL-31 IL-28A/IFNλ2 IL-23 IL-22 IL-21 IL-17E/IL-25 VEGF-A TNFα TGFα MIP-1β/CCL4 MIP-1α/CCL3 MCP-1/CCL2 IL-18 IL-17A/CTLA8 IL-15 IL-13 IL-12/23 (p40) (LTA) (mature) ▼ (Cat. No.(Cat. STTHMAG-21K) Hormone Multi-Species Steroid/Thyroid No.(Cat. NHPPT1MG-46K) Panel 1 Non-Human PrimatePituitary No.(Cat. NHPMHMAG-45K) Hormone Metabolic Non-Human Primate Endocrinology Metabolism/ Panel 1 Mouse Cytokine/Chemokine Immunology MOUSE No.(Cat. MSHMAG-21K) Panel Multi-Species Hormone 32 32 25 25 IL-12 (p70) IL-12 (p40) IL-10 IL-9 IL-7 IL-6 IL-5 IL-4 IL-3 IL-2 CNTF Agouti-Related ACTH IL-6 Glucagon GLP-1 (active) GIP (total) Ghrelin (active) C-Peptide Amylin (active) IL-1β IL-1α IFNγ GM-CSF G-CSF Eotaxin/CCL11 Progesterone Estradiol Cortisol Progesterone Estradiol Cortisol

Protein (AgRP) (Bulk Cat. No. (BulkCat. MCYTMAG70PMX32BK) No. (Cat. MCYTMAG-70K-PX32) (Bulk Cat. No.(Bulk Cat. MCYTMAG70PMX25BK) No.(Cat. MCYTOMAG-70K-PMX) (Cat. No.(Cat. MCYTOMAG-70K) ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ■ ◗

^ ♦ ♦ ■ ◗

◗ ^ ◗ VEGF-A TNFα RANTES/CCL5 MIP-2/CXCL2 MIP-1β/CCL4 MIP-1α/CCL3 MIG/CXCL9 M-CSF MCP-1/CCL2 LIX LIF KC/GROα/CXCL1 IP-10/CXCL10 IL-17A/CTLA8 IL-15 IL-13 TSH LH GH FSH PYY (total) Pancreatic MCP-1/CCL2 Leptin Insulin T4 T3 Testosterone T4 T3 Polypeptide (PP) NEW ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ Mouse High Sensitivity TCell Mouse HighSensitivity Panel 2 Mouse Cytokine/Chemokine Mouse Th17 Receptor Mouse SolubleCytokine (Cat. No.(Cat. MHSTCMAG-70K) 18 18 15 15 25 25 IL-7 IL-6 IL-5 IL-4 IL-2 IL-1β IL-1α IFNγ GM-CSF IL-16 IL-11 IFNγ IFNβ1 Fractalkine/CX3CL1 Exodus-2/ (EPO) Erythropoietin IL-17A/CTLA8 IL-15 IL-13 IL-12 (p70) IL-10 IL-6 IL-5 IL-4 IL-2 IL-1β IFNγ GM-CSF sCD40L sIL-6R sIL-4R sIL-2Rα sIL-1RII sIL-1RI sGP130 sCD30

CCL21/6Ckine

(Bulk Cat. No.(Bulk Cat. MHSTCMAG-70KPXBK) No.(Cat. MHSTCMAG-70KPMX) No.(Bulk Cat. MECY2MAG73KPXBK) No.(Cat. MECY2MAG-73KPX) (Bulk Cat. No.(Bulk Cat. MT17MAG47PMX25BK) No.(Cat. MT17MAG47K-PX25) (Cat. No.(Cat. MECY2MAG-73K) (Cat. No.(Cat. MTH17MAG-47K) No.(Cat. MSCRMAG-42K) TNFα MIP-2/CXCL2 MCP-1/CCL2 LIX KC/GROα/CXCL1 IL17A/CTLA8 IL-13 IL-12 (p70) IL-10 TIMP-1 TARC/CCL17 MIP-3β/CCL19 MIP-3α/CCL20 MCP-5/CCL12 MDC/CCL22 IL-20 IL-17A/F TNFβ/Lymphotoxin-α TNFα MIP-3α/CCL20 IL-33/NF-HEV IL-31 IL-28B/IFNλ3 IL-27 IL-23 IL-22 IL-21 IL-17F IL-17E/IL-25 sVEGFR3/sFlt-4 sVEGFR2/sKDR/sFlk-1 sVEGFR1/sFlt-1 sTNF RII sTNF RI sRAGE (LTA) (mature)

MOUSE

MILLIPLEX® map CVD / IMMUNOLOGY / METABOLISM / ENDOCRINOLOGY For Research Use Only. ^

■ NEW (SAP) (OSTN) Testosterone T3 T4 (total) PAI-1 sP-Selectin Thombomodulin Oncostatin M PLGF-2 I (TnI) Troponin T (TnT) Troponin CRP Haptoglobin P Serum Amyloid Irisin LIF Myostatin/GDF8 Oncostatin-M (OSM) Osteocrin/Musclin Osteonectin/SPARC LH Prolactin TSH T3 T4 ^

■ Serum/Plasma only samples only Tissue Culture format assay Competitive Available for custom premix Available | 11 MerckMillipore.com/milliplex

(AGP) Protein 1 (FSTL1) ● ★ ^ Cortisol Estradiol Progesterone sCD31/sPECAM-1 sE-Selectin sICAM-1 ProMMP-9 sCD40L CXCL16 Endocan-1 Follistatin LIGHT D Adipsin/Factor Glycoprotein α-1-Acid α-2-Macroglobulin BDNF Erythropoietin (EPO) ★ FGF-21 Fractalkine/CX3CL1 Follistatin-like IL-6 IL-15 ACTH BDNF FSH GH Cortisol Estradiol Progesterone (Cat. No. MSHMAG-21K) MSHMAG-21K) (Cat. No. Cardiovascular 1 Mouse CVD Panel MCVD1MAG-77K) (Cat. No. 2 Mouse CVD Panel MCVD2MAG-77K) (Cat. No. 2 Mouse Acute Phase Panel MAP2MAG-76K) (Cat. No. Mouse Myokine Mouse MMYOMAG-74K) (Cat. No. Mouse Pituitary MPTMAG-49K) (Cat. No. Multi-Species Hormone Steroid/Thyroid STTHMAG-21K) (Cat. No. Hormone Multi-Species Panel Polypeptide (PP) Polypeptide (PP) Polypeptide Leptin Pancreatic (total)PYY Leptin MCP-1/CCL2 Pancreatic (total)PYY Resistin TNFα PAI-1 (total) PAI-1 Resistin TNFα PAI-1 (total) PAI-1 Resistin TNFα ◗ ◗ ◗ ◗ ◗ ◗ ◗ These analytes cannot be plexed with otherThese analytes cannot be plexed analytes in this panel in serum/plasma a protease inhibitor during Requires sample collection sample extraction Requires ▲  ◗ ■ GIP (total) (active) GLP-1 Insulin GIP (total) (active) GLP-1 Glucagon IL-6 Insulin (active) Amylin (active) Ghrelin (active) Amylin 2 C-Peptide (active) Ghrelin Adiponectin IL-6 Leptin MCP-1/CCL2 Adiponectin IL-6 Insulin Leptin MCP-1/CCL2 IgE (Cat. No. MGTMAG-78K) (Cat. No. Mouse Metabolic Hormone MMHMAG-44K) (Cat. No. (Cat. No. MADCYMAG-72K) (Cat. No. Mouse Adiponectin – Singleplex (Serum/Plasma samples) MADPNMAG-70K-01) (Cat. No. Mouse Gut Hormone Metabolism/ Endocrinology Mouse Adipokine (Serum/Plasma samples) MADKMAG-71K) (Cat. No. Mouse Adipocyte samples) (Cell culture Mouse IgE – Singleplex IgE Mouse MGAMMAG-300E) (Cat. No.

map kits ▼ ® IgG2b IgG3 IgM MMP-12 proMMP-9 MMP-12 MMP-8 TGFβ3 IL-4 IL-5 IL-6 IL-10 IL-13 MIP-1β/CCL4 RANTES/CCL5 TNFα Analytes which cannot be plexed together:Analytes which cannot be plexed – Active and total – Active and total – Free listed in Cat. No. Available Premix panel only Premix

▼ † Legend key for MILLIPLEX key Legend ♦ (Cat. No. MGAMMAG-300K) (Cat. No. (Cat. No. MCD8MAG-48K) (Cat.No. (Bulk Cat. No. MCD8MAG48KPX15BK) (Bulk Cat. No. (Cat. No. MCD8MAG48K-PX15) (Cat.No.

TNFRSF9

IgA IgG1 IgG2a MMP-2 MMP-3 MMP-8 proMMP-9 MMP-2 MMP-3 TGFβ1 TGFβ2 TGFβ1 sFas/TNFRSF6 sFasL GM-CSF Granzyme B IFNγ IL-2 sCD137/4-1BB/ NON-CONFIGURABLE KIT 15 15 Immune Response Mouse Immunoglobulin Isotyping Mouse MMP Panel 3 Mouse MMP Panel samples) (Cell culture MMMP3MAG-79K) (Cat. No. (Cat. No. MMMP2MAG-79K) (Cat. No. Mouse MMP Panel 2 Mouse MMP Panel (Serum/Plasma samples) (Cat. No. MMMP1MAG-79K) (Cat. No. Mouse MMP Panel 1 Mouse MMP Panel (Serum/Plasma samples) (Cat. No. TGFBMAG-64K-03) (Cat. No. Multi-Species TGFβ – 3 Plex TGFβ Multi-Species (Cat. No. TGFBMAG-64K-01) (Cat. No. TGFBMAG-64K-01BK) (Bulk Cat. No. Multi-Species TGFβ – TGFβ Multi-Species Singleplex Mouse CD8+ T Cell CD8+ Mouse MOUSE

For Research Use Only. MILLIPLEX® map BONE / CANCER / CVD / IMMUNOLOGY / METABOLISM / ENDOCRINOLOGY / NEUROSCIENCE / TOXICITY 12 |MerckMillipore.com/milliplex Factor Panel 1 Angiogenesis /Growth Mouse Cancer No.(Cat. MBNMAG-41K) Mouse Bone Bone Metabolism (Cat. No.(Cat. MKI2MAG-94K) PanelMouse Kidney Injury 2 No.(Cat. MKI1MAG-94K) PanelMouse Kidney Injury 1 Toxicity No.(Cat. RMNPMAG-83K) Mouse Neuropeptide No.(Cat. MABMAG-83K) Mouse Amyloid Beta Neuroscience No.(Cat. MAGPMAG-24K) EGF Cystatin C Clusterin KIM-1 IP-10/CXCL10 β-2-Microglobulin Neurotensin β-Endorphin α-MSH Amyloid 1-40 beta IL-1β HGF G-CSF Follistatin (FST) FGF-2/FGF-basic sFasL Endothelin-1 Endoglin EGF ▲ sCD31/sPECAM-1 ▲ Betacellulin Angiopoietin-2 ▲ Amphiregulin sALK-1 Insulin IL-6 FGF-23 DKK1 ACTH Osteopontin (OPN) NGAL/Lipocalin-2 VEGF-A TIMP-1 Renin P Substance Oxytocin Orexin A Amyloid 1-42 beta VEGF-D VEGF-C VEGF-A TNFα SDF-1/CXCL12 Prolactin Growth Placental MIP-1α/CCL3 MCP-1/CCL2 Leptin KC/CXCL1 IL-17A/CTLA8 IL-6 TNFα Sclerostin (SOST) Osteoprotegerin Leptin Factor (PLGF-2) (OPG) NEW ■

^ (Bulk Cat. No.(Bulk Cat. TGFBMAG-64K-01BK) No.(Cat. TGFBMAG-64K-01) Singleplex Multi-Species TGFβ – Rat Cytokine/Chemokine Immunology RAT (Cat. No.(Cat. RMHMAG-84K) Hormone Rat Metabolic No.(Cat. RADPCMAG-82K) culture(Cell samples) Rat Adipocyte No.(Cat. RADPKMAG-80K) (Serum/Plasma samples) Rat Adipokine Endocrinology Metabolism/ No.(Cat. TGFBMAG-64K-03) Multi-Species TGFβ –3Plex 27 27 27 NON-CONFIGURABLE KIT IL-6 IL-5 IL-4 IL-2 IL-1β IL-1α IFNγ GROα /KC /CINC- GM-CSF G-CSF Fractalkine /CX3CL1 /CCL11 Eotaxin EGF IL-6 Glucagon GLP-1 (active) GIP (total) Ghrelin (active) C-Peptide 2 Amylin (active) Leptin IL-6 IL-1β Adiponectin Leptin Insulin IL-6 IL-1β TGFβ2 TGFβ1 TGFβ1

1 /CXCL1

(Bulk Cat. No.(Bulk Cat. RECYMAG65PMX27BK) No.(Cat. RECYMAG65K27PMX) (Cat. No.(Cat. RECYTMAG-65K) ◗ ◗ ◗ ◗ VEGF TNFα RANTES/CCL5 MIP-2/CXCL2 MIP-1α/CCL3 MCP-1/CCL2 LIX Leptin IP-10/CXCL10 IL-18 IL-17A/CTLA8 IL-13 IL-12 (p70) IL-10 TNFα PYY (total) Pancreatic MCP-1/CCL2 Leptin Insulin TNFα PAI-1 (total) MCP-1/CCL2 TNFα PAI-1 (total) MCP-1 /CCL2 TGFβ3 Polypeptide (PP) ▼ (Cat. No.(Cat. STTHMAG-21K) Hormone Steroid /Thyroid Multi-Species No.(Cat. RSHMAG-69K) Rat Stress Hormone No.(Cat. RPTMAG-86K) Rat Pituitary No.(Cat. RMYOMAG-88K) Rat Myokine (Cat. No.(Cat. RV1MAG-26K) (Serum/Plasma samples) Rat Vascular Panel Injury 1 No.(Cat. RCI1MAG-87K) Panel Injury 1 Rat Cardiac Cardiovascular No.(Cat. RTHYMAG-30K) Rat Thyroid No.(Cat. MSHMAG-21K) Panel Multi-Species Hormone Corticosterone^ ACTH GH FSH BDNF ACTH GDF8/Myostatin FSTL1 Fractalkine FGF-21 Erythropoietin BDNF GROα /KC /CINC- Connective Tissue Caveolin-1 Creatine Kinase TroponinCardiac T TroponinCardiac I T4^ T3^ Progesterone Estradiol Cortisol Progesterone Estradiol Cortisol 1 /CXCL1 (CTGF) FactorGrowth Muscle (CKM) (cTnT) (cTnI) ■

^ Melatonin^ TSH Prolactin LH Osteonectin/SPARC Osteocrin (OSTN) LIF Irisin IL-15 IL-6 TSH T4 T3 Testosterone T4^ T3^ VEGF TNFα TIMP-1 PAI-1 (total) MCP-1 / CCL2 IL-6 TIMP-1 Myosin LightChain3 Follistatin-like Protein FABP3 (MYL3) 1 (FSTL1) NEW NEW ■ ■

^ RAT

MILLIPLEX® map BONE / IMMUNOLOGY / METABOLISM / ENDOCRINOLOGY / NEUROSCIENCE / TOXICITY For Research Use Only.

^

■ NEW RBP4 TFF-3 IL-13 IL-18 KC/GRO / CCL2 MCP-1 PDGF-BB RANTES / CCL5 SCF / CXCL12 SDF-1 TNFα T3 T4 Testosterone T3 T4 / CCL2 MCP-1 NGAL / Lipocalin-2 Osteopontin (OPN) ^

■ Serum/Plasma only samples only Tissue Culture format assay Competitive Available for custom premix Available | 13 MerckMillipore.com/milliplex (Cat. No. FCYTMAG-20K-PMX) (Cat. No. FCYTMAG20KPX19BK) (Bulk Cat. No.

● ★ ^ Albumin β-2-Microglobulin sFAS/TNFRSF6 Flt3 Ligand GM-CSF IFNγ IL-1β IL-2 IL-4 IL-6 / CXCL8 IL-8 (p40) IL-12 Cortisol Estradiol Progesterone Cortisol Estradiol Progesterone Clusterin C Cystatin / CXCL8 IL-8 KIM-1 NON-CONFIGURABLE KIT 19 19 Canine Kidney Toxicity Panel 2 Panel Canine Toxicity Kidney (Urine samples) CKT2MAG-97K) (Cat. No. Immunology Cytokine/ChemokineFeline Multi-Species Multi-Species Hormone Steroid/Thyroid STTHMAG-21K) (Cat.No. Hormone Multi-Species Panel MSHMAG-21K) (Cat. No. Toxicity Canine Toxicity Kidney 1 Expanded Panel (Urine samples) CKT1MAG-97K) (Cat. No. ▼ Polypeptide (PP) Polypeptide TGFβ3 Insulin Leptin Pancreatic (total)PYY GH TSH GSTα SDH IL-15 IL-18 / CXCL10 IP-10 KC-like / CCL2 MCP-1 TNFα ◗ These analytes cannot be plexed with otherThese analytes cannot be plexed analytes in this panel in serum/plasma a protease inhibitor during Requires sample collection sample extraction Requires (Cat. No. CCYTOMAG-90K) (Cat. No. (Cat. No. CCYTMG-90K-PX13) (Cat. No. CCYTMAG90KPX13BK) (Bulk Cat. No.

▲  ◗ ■

GIP (total) ◗ (active) GLP-1 Glucagon ACTH BDNF FSH TGFβ1 TGFβ2 (total) ◗ Amylin (active) Ghrelin TGFβ1 GM-CSF IFNγ IL-2 IL-6 IL-7 / CXCL8 IL-8 IL-10 5'NT/CD73 ARG1 GOT1 NON-CONFIGURABLE KIT 13 13 Canine Pituitary CPTMAG-96K) (Cat. No. (Cat. No. TGFBMAG-64K-03) (Cat. No. Metabolism/ Endocrinology Canine Gut Hormone CGTMAG-98K) (Cat. No. Multi-Species TGFβ – TGFβ Multi-Species Singleplex TGFBMAG-64K-01) (Cat. No. TGFBMAG-64K-01BK) (Bulk Cat. No. – 3 Plex TGFβ Multi-Species OTHER SPECIES OTHER Immunology Canine Cytokine/Chemokine Rat Liver InjuryRat Liver RLI1MAG-92K) (Cat. No.

map kits ® ▲ ^

■ (vWF) Cystatin C Cystatin EGF NGAL / Lipocalin-2 KIM-1 Osteopontin (OPN) TIMP-1 VEGF-A Orexin A Orexin Oxytocin Substance P Fibrinogen Haptoglobin Leptin Osteoprotegerin (OPG) PTH Sclerostin (SOST) sICAM-1 Willebrand Factor von Analytes which cannot be plexed together:Analytes which cannot be plexed – Active and total – Active and total – Free listed in Cat. No. Available Premix panel only Premix

▼ † Legend key for MILLIPLEX key Legend ♦ (AGP) (A2M) (AGP) α-1-Acid Glycoprotein Glycoprotein α-1-Acid Albumin^ β-2-Microglobulin Calbindin Clusterin GSTα^ / CXCL10 IP-10 α-MSH β-Endorphin Neurotensin ACTH DKK1 FGF-23 Insulin α-2-Macroglobulin α-1-Acid Glycoprotein Glycoprotein α-1-Acid Adiponectin sE-Selectin (Cat. No. RKTX2MAG-37K) (Cat. No. Rat Kidney Toxicity Panel 2 Panel Toxicity Rat Kidney (Urine samples) Rat Kidney Toxicity Panel 1 Panel Toxicity Rat Kidney (Urine samples) RKTX1MAG-37K) (Cat. No. Toxicity Rat Neuropeptide RMNPMAG-83K) (Cat. No. Neuroscience Rat Bone Panel 1 Rat Bone Panel (Serum/Plasma samples) RBN1MAG-31K) (Cat. No. Bone Metabolism (Cat. No. RV3MAG-26K) (Cat. No. Rat Vascular Injury Panel 3 Injury Panel Rat Vascular (Serum/Plasma samples) Rat Vascular Injury Panel 2 Injury Panel Rat Vascular samples) (Serum/Plasma RV2MAG-26K) (Cat.No. OTHER SPECIES

For Research Use Only. MILLIPLEX® map IMMUNOLOGY / METABOLISM / ENDOCRINOLOGY 14 |MerckMillipore.com/milliplex (Cat. No.(Cat. STTHMAG-21K) Hormone Multi-Species Steroid/Thyroid No.(Cat. FMHMAG-29K) Feline Hormone Metabolic Endocrinology Metabolism/ (Cat. No.(Cat. TGFBMAG-64K-03) Multi-Species TGFβ –3Plex No.(Bulk Cat. TGFBMAG-64K-01BK) No.(Cat. TGFBMAG-64K-01) Singleplex Multi-Species TGFβ - Porcine Cytokine/Chemokine Immunology No.(Cat. MSHMAG-21K) Panel Multi-Species Hormone 13 13 NON-CONFIGURABLE KIT TGFβ2 TGFβ1 TGFβ1 IL-4 IL-2 IL-1Ra IL-1β IL-1α IFNγ GM-CSF Progesterone Estradiol Cortisol Progesterone Estradiol Cortisol Glucagon GLP-1 (active) GIP (total) Ghrelin (active) Amylin (active)

(Bulk Cat. No.(Bulk Cat. PCYTMAG23PMX13BK) No.(Cat. PCYTMG-23K-13PX) (Cat. No.(Cat. PCYTMAG-23K) ♦ ▼ † Legend key forMILLIPLEX

Premix panelonly Available No. inCat. listed –Free andtotal –Active andtotal Analytes whichcannotbeplexed Analytes together: ■ ◗

^ ■ ◗

◗ ^ ◗ TGFβ3 TNFα IL-18 IL-12 IL-10 IL-8/CXCL8 IL-6 T4 T3 Testosterone T4 T3 PYY (total) Pancreatic Leptin Insulin Polypeptide (PP) NEW ® ▼ mapkits

Panel Equine Cytokine/Chemokine Immunology No.(Cat. MSHMAG-21K) Panel Multi-Species Hormone No.(Cat. STTHMAG-21K) Hormone Multi-Species Steroid/Thyroid Endocrinology Metabolism/ 23 23 Progesterone Estradiol Cortisol Progesterone Estradiol Cortisol IL-5 IL-4 IL-2 IL-1β IL-1α IFNγ GRO GM-CSF G-CSF Fractalkine/CX3CL1 FGF-2/FGF-basic Eotaxin/CCL11

■ ◗ ▲ 

What’s intheMILLIPLEX (Cat. No.(Cat. EQCTMG93KPX23BK) No.(Cat. EQCYTMG-93KPX23) (Cat. No.(Cat. EQCYTMAG-93K) Plate Sealers Griener BlackMylar Requires sample extraction sample collection Requires aproteaseinhibitorduring inthispanelserum/plasma analytes cannotbeplexedThese analytes withother 96-well Plate ■

^ Detection Antibody ■

^ Cocktail T4 T3 Testosterone T4 T3 TNFα RANTES/CCL5 MCP-1/CCL2 IP-10/CXCL10 IL-18 IL-17A/CTLA8 IL-13 IL-12 (p70) IL-10 IL-8/CXCL8 IL-6 NEW Serum Matrix ® kit? Protocol Included 10X Wash Buffer Panel Multi-Species Hormone No.(Cat. STTHMAG-21K) Hormone Multi-species Steroid/Thyroid Endocrinology Metabolism/ No.(Cat. TGFBMAG-64K-03) Multi-Species TGFβ -3Plex No.(Bulk Cat. TGFBMAG-64K-01BK) No.(Cat. TGFBMAG-64K-01) Singleplex Multi-Species TGFβ – (Cat. No.(Cat. MSHMAG-21K) Bead Diluent NON-CONFIGURABLE KIT Progesterone Estradiol Cortisol TGFβ2 TGFβ1 TGFβ1 Progesterone Estradiol Cortisol ^ ★ ● Assay Buffer Assay

Available forcustom premix Competitive assay format Tissue Culture samplesonly Serum/Plasma only ■

^ Streptavidin Phycoerythrin ■

^ SAPE Capture antibody-conjugated QC1 &QC2 Controls T4 T3 TGFβ3 T4 T3 Testosterone Standard Magnetic Beads beads insolution NEW

▼ CELL SIGNALING

MILLIPLEX® map CELL SIGNALING / MAPmate™ KITS For Research Use Only.

® | 15 MerckMillipore.com/milliplex map Cell Signaling kits* ® Cell Signaling Kit* Cardiovascular and metabolic health andCardiovascular disease - Immune response -  - Neurological disorders and cancer

Plex up to 8 individual MAPmate™ kits to design Plex up to 8 individual MAPmate™ using our Cell Signaling assays own custom your Buffer and Detection Kit* kits into existing MAPmate™ Plex additional MILLIPLEX Measure multiple total or phosphoproteinsMeasure simultaneously in a single sample a deeper understandingAcquire of intracellular in both normal and disease involved pathways states that include: Use β–tubulin or GAPDH as housekeeping/loading Use β–tubulin or GAPDH as housekeeping/loading plexing into an existing MILLIPLEX controls by map Refer to the guidelines provided in the protocols. in the protocols. to the guidelines provided Refer • • * Cell & signaling phosphoprotein total multiplex assays • • phosphoprotein & total MAPmate™ singleplex kits •

® map assays

® platform ® xMAP ® map cell signaling multiplex

® platform, to better understand cell signaling

®

All 2-plex assays (exceptions noted) can be noted) (exceptions All 2-plex assays totalcombined with each other to study multiple and phosphorylated well proteins in the same same well Directly compare the total vs. phosphoprotein the total compare vs. Directly them in the reading by assay in your levels pathways. Multiplexing, using the Luminex panels and MAPmate™ assays, using the Luminex assays, panels and MAPmate™ (see instrumentation section), provides faster(see instrumentation section), provides compared cell signaling questions answers to your mass spectrometry blots, to traditional Western that require phosphorylation assays and radioactive large amounts of sample. • • Cell + signaling phosphoprotein total assays 2-plex Discovering the pathway Discovering often interconnected and are Signaling pathways Count on our mechanisms. multifaceted involve to enable accurate assays cell signaling multiplex quantitation of both total and phosphorylated forms connections and revealing of signaling proteins, Choose of interest. pathways crosstalk within your from MILLIPLEX xMAP Cell MILLIPLEX signaling Communicate Uncover How Your Cells Your How Uncover CELL SIGNALING

For Research Use Only. MILLIPLEX® map CELL SIGNALING 16 |MerckMillipore.com/milliplex (Cat. No.(Cat. 48-6 Akt1 Phospho/Total No.(Cat. 48-618MAG) AB2▼✸●● Akt Phospho/Total SignalingPhosphoprotein+TotalCell 2PlexAssays (Cat. No.(Cat. 48-619MAG) AB2▼●● Erk/MAPK 1/2Phospho/Total No.(Cat. 48-628MAG) AB2▼●● CREB Phospho/Total No.(Cat. 48-633MAG) AB2▼✸●● Akt3 Phospho/Total No.(Cat. 48-632MAG) AB2▼✸●● Akt2 Phospho/Total Analyte Akt/PKB Analyte Analyte CREB CREB Analyte Analyte Analyte Akt1 Erk/MAPK 1/2 Akt3 Akt2 Akt1 Akt/PKB Erk/MAPK 1/2 Akt3 Akt2 ▼ ✸ ●●

Premix panelonly Can beplexedCan withother2Plexes Cannot plexwith otherphosphoAkt Cannot 3 1MAG) AB2▼✸●● Total Total Total Total Total Total 3 3 3 3 3 3 – – – – 2Plex – 2Plex 2Plex 2Plex 2Plex 3 (Ser473) Phosphorylated (Ser473) Phosphorylated 3 (Thr185/Tyr187) Phosphorylated (Ser472) Phosphorylated (Ser474) Phosphorylated NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT 3 (Ser133) Phosphorylated NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT

– 2Plex AB2: AB1: H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R Uses Assay Buffer2 Uses Assay Buffer1 (Cat. No.(Cat. 48-625MAG) AB2▼●● mTOR Phospho/Total No.(Cat. 48-622MAG) AB2▼●● JNK Phospho/Total No.(Cat. 48-626MAG) AB2▼●● IRS1 Phospho/Total (Cat. No.(Cat. 48-623MAG) AB2▼●● STAT3 Phospho/Total No.(Cat. 48-624MAG) AB1orAB2▼●● p38 Phospho/Total mTOR Analyte JNK/SAPK1 Analyte IRS1 Analyte STAT3 Analyte p38/SAPK2A/B Analyte mTOR JNK/SAPK1 IRS1 STAT3 p38/SAPK2A/B R Rat M Mouse H Human Total Total Total Total Total 3 3 3 3 3 – – – 2Plex 2Plex – – 2Plex 2Plex 3 (Ser2448) 3 (Thr183/Tyr185) 3 (Ser636) Phosphorylated Phosphorylated Phosphorylated 3 (Tyr705) 3 (Thr180/Tyr182) Phosphorylated Phosphorylated 2Plex NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT H, M,R H, M,R H, M H, M H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R MILLIPLEX® map CELL SIGNALING For Research Use Only. H, M H, M, R H H, M H, M, R H, M, R H H, M, R H, M, R H H H H H H H H H H H H, M, R H H H H H H, M, R H H, M, R H H, M, R H, M, R H H H, M 7 Plex – 10 Plex – 3 (Ser212) 3 (Thr180/Tyr182) 3 (Ser15) 3 (Tyr701) 3 (Ser73) 3 (Thr185/Tyr187) 3 (Ser78) 3 (Thr183/Tyr185) 3 (Ser222) Phosphorylated (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) Phosphorylated 3 (Ser345) 3 (Thr68) 3 (Ser139) 3 (Ser15) Phosphorylated 3 (Thr71) NON-CONFIGURABLE KIT NON-CONFIGURABLE NON-CONFIGURABLE KIT

| 17 MerckMillipore.com/milliplex 3 3 3 Total Total Total p38/SAPK2A/B p53 STAT1 Erk/MAPK 1/2 HSP27 JNK/SAPK1 MEK1 MSK1 Analyte c-Kit c-Met/HGFR ErbB1/EGFR ErbB2/HER2 ErbB3/HER3 ErbB4/HER4 FGFR1 Flt3 IGF1R IR MSCFR PDGFRα PDGFRβ TIE1 TIE2 VEGFR1/Flt-1 VEGFR2/KDR/Flk-1 VEGFR3/Flt-4 Analyte ATR Chk1 Chk2 H2A.X MDM2 p21 p53 Analyte ATF2 c-Jun Human RTK (Phosphoprotein) Human RTK (Choose Analytes Needs) that Meet your AB1 HPRTKMAG-01K) (Cat. No. Human DNA Damage/Genotoxicity Damage/Genotoxicity Human DNA AB1 ▼ 48-621MAG) (Cat.No. MAPK/SAPK (Phosphoprotein) AB2 ▼ 48-660MAG) (Cat. No. H, M, R H H H H H, M, R H H, M, R H, M, R H, M, R H, M, R H, R H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R H, M H H, R H, M H, M, R H, M, R H, M, R H, M, R 11 Plex – Phosphorylated 3 (Ser473) 3 (Ser112) 3 (Ser70) 3 (Thr183/Tyr185) 3 (Ser46) Phosphorylated Phosphorylated 3 (Ser473) 3 (Ser21) 3 (Ser9) 3 (Tyr1135/1136) 3 (Tyr1162/1163) 3 (Ser636) 3 (Ser2448) 3 (Thr389/412) 3 (Ser380) 3 (Ser235/236) 3 (Ser939) NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE 11 Plex 3 3 3 3 3 3 3 3 3 3 3 3 3 – Total Total Total 7 Plex – Analyte Akt/PKB BAD Bcl-2 Caspase 8 Active Caspase 9 Active JNK/SAPK1 p53 Analyte Akt/PKB GSK3α GSK3β IGF1R IR IRS1 mTOR p70S6 Kinase PTEN RPS6 TSC2 Analyte Akt/PKB GSK3α GSK3β IGF1R IR IRS1 mTOR p70S6 Kinase PTEN RPS6 TSC2 Early Apoptosis ▼ AB2 48-669MAG) (Cat. No. Akt/mTOR (Total) (Total) Akt/mTOR ▼ AB2 48-612MAG) (Cat. No. Akt/mTOR (Phosphoprotein) (Phosphoprotein) Akt/mTOR AB2 ▼ 48-611MAG) (Cat.No. Cell Signaling For Research Use Only. MILLIPLEX® map CELL SIGNALING 18 |MerckMillipore.com/milliplex (Cat. No.(Cat. 48-655MAG) AB2▼ Protein Translation —6Plex No.(Cat. 48-630MAG) AB1▼ NFκB —6Plex No.(Cat. 48-681MAG) AB2▼ Multi-Pathway (Total) —9Plex (Cat. No.(Cat. 48-680MAG) AB2▼ Multi-Pathway (Phosphoprotein)—9Plex Signaling(continued) Cell TNFR1 NFκB 4E-BP1 elF-4G elF-4E elF-4B IKKα/β elF2a IκBα Analyte FADD c-Myc Analyte Analyte STAT5A/B STAT3 p70S6 Kinase p38/SAPK2A/B NFκB JNK/SAPK1 Erk/MAPK 1/2 CREB Akt/PKB STAT5A/B STAT3 p70S6 Kinase p38/SAPK2A/B NFκB JNK/SAPK1 Erk/MAPK 1/2 CREB Akt/PKB Analyte Total Total Total Total 3 3 3 3 3 3 3 3 3 3 3 3 NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT 3 (Tyr694/699) 3 (Ser727) 3 (Thr389/412) 3 (Thr180/Tyr182) 3 (Ser536) 3 (Thr183/Tyr185) 3 (Thr185/Tyr187) 3 (Ser133) 3 (Ser473) Phosphorylated 3 (Ser536) 3 (Ser177/181) 3 (Ser32) 3 (Ser194) Phosphorylated Phosphorylated 3 (Thr37/46) 3 (Ser1108) 3 (Ser209) 3 (Ser422) 3 (Ser51) Phosphorylated

H H, M H H, M,R H, M,R H, M,R H, M,R H, M,R H H H H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H H, M,R H, M,R H, M,R H, M,R (Cat. No.(Cat. 48-610MAG) AB2▼ STAT (Phosphoprotein)—5Plex No.(Cat. 48-650MAG) AB2▼ (Phosphoprotein) —8Plex Src Family KinaseActive Site (Cat. No.(Cat. 48-614MAG) AB2▼ TGFβ —6Plex No.(Cat. 48-690MAG) AB2▼ T-Cell Receptor (Phosphoprotein)—7Plex STAT1 STAT2 Analyte Yes Src Lyn Lck Hck Fyn Fgr Blk Analyte TGFβRII SMAD4 SMAD3 SMAD2 Erk/MAPK 1/2 Akt/PKB Analyte Analyte CD3ε STAT3 CREB STAT5A/B Erk/MAPK 1/2 STAT6 LAT Lck Syk ZAP-70 Total Total Total Total 3 3

NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT 3 (Ser423/425) 3 (Ser465/467) 3 (Thr185/Tyr187) 3 (Ser473) Phosphorylated NON-CONFIGURABLE KIT 3 (Tyr421) 3 (Tyr419) 3 (Tyr397) 3 (Tyr394) 3 (Tyr411) 3 (Tyr420) 3 (Tyr412) 3 (Tyr389) Phosphorylated 3 (Tyr701) Phosphorylated 3 (Tyr690) 3 (panTyr) Phosphorylated 3 (Tyr705) 3 (Ser133) 3 (Tyr694/699) 3 (Thr185/Tyr187) 3 (Tyr641) 3 (panTyr) 3 (panTyr) 3 (panTyr) 3 (panTyr) H, M H, M,R H, M,R H, R H, M,R H, M H, M,R H, R H H H, M,R H, M,R H, M,R H, M,R H H H H, M,R H, M,R H, M,R H, M,R H H H, M,R H H MILLIPLEX® map CELL SIGNALING / MAPmate™ KITS For Research Use Only. 47-208 47-220 47-206 47-216 47-219 47-224 47-235 47-238 47-207 Cat. No. Cat. No. 46-654MAG 46-654MAG 46-655MAG 46-655MAG 46-656MAG 46-656MAG 46-612MAG 46-612MAG 46-610MAG 46-610MAG 46-685MAG 46-685MAG 46-686MAG 46-686MAG 46-618MAG 46-618MAG 46-613MAG 46-613MAG 46-609MAG 46-609MAG 46-602MAG 46-602MAG 46-604MAG 46-604MAG 46-694MAG 46-694MAG 46-675MAG 46-675MAG 46-677MAG 46-677MAG 46-667MAG 46-667MAG 46-713MAG 46-713MAG H, M H, M H H, M, R H, M, R H, M, R H, M, R H, M H, M H, M, R H, M, R H, M H, M H, M, R H, M, R H H, M, R Species Homology | 19 MerckMillipore.com/milliplex 2 O 2 STAT1 (total) STAT1 STAT1 (Tyr701) STAT1 Cleaved PARP (total) PARP Cleaved p38/SAPK2A/B (total) p38/SAPK2A/B (Thr180/Tyr182) mTOR (total) mTOR mTOR (Ser2448) mTOR JNK/SAPK1 (total) JNK/SAPK1 (Thr183/Tyr185) ERK/MAPK 1/2 (total) ERK/MAPK 1/2 (Thr185/Tyr187) Caspase 3 (Active) BAD (Ser112) BAD Akt/PKB (total) Akt/PKB (Ser473) GAPDH (total) β-Tubulin (total) β-Tubulin MAPmate™ Kits MAPmate™ Jurkat: Paclitaxel Jurkat: Unstim Jurkat: MCF7: IGF-1 NIH3T3: Anisomycin Ramos: PVD Lysate Description Lysate HepG2: TGFβ HUVEC: Serum Anisomycin Jurkat: H Jurkat: 47-210 47-218 47-217 47-226 47-229 47-230 47-205 47-211 47-232 47-227 Cat. No. Cell Signaling Panels to enhance the panel or serve as the panel or serve to enhance Panels map Cell Signaling ® GAPDH and β-Tubulin MAPmates™ can be used MAPmates™ GAPDH and β-Tubulin of the MAPmates™. for normalization with any Phospho-specific and total MAPmate™ pairs. pairs. Phospho-specific and total MAPmate™ – – – – All MAPmate™ assays require the Cell Signaling the require assays All MAPmate™ ). This kit Kit (48-602MAG Buffer & Detection thecontains all necessary except reagents and flat bottom Both a filter assay. MAPmate™ included for convenience. plate are Signaling Assay use Cell assays All MAPmate™ Buffer 2 (AB2). should not be assays The following MAPmate™ together: plexed Consult the protocol prior to use. Consult the protocol A431: EGF A549: Camptothecin Daudi: IL-4 HeLa: IFNα HeLa: Lambda Phosphatase HeLa: TNFα+CalA HeLa: Unstim HeLa: HS/Ars HepG2: DCA HepG2: Insulin Lysate Description Lysate Lysates • • • Important MAPmate™ Guidelines Important MAPmate™ • Cell (continued) Signaling Singleplex Assays & Total Phosphoprotein MAPmate™: Kit, and Detection Cell Signaling Buffer together using our assays 8 individual MAPmate™ Plex up to them in existing MILLIPLEX or include controls within the guidelines provided in the protocols. All of the analytes in the MAPmate™ portfolio All of the analytes in the MAPmate™ in the protocols. provided the guidelines controls within or email sales specialist contact information your more For into custom assays. to build available are . us at [email protected] For Research Use Only. LUMINEX® TECHNOLOGY 20 |MerckMillipore.com/luminex All systems runontheLuminex Analyst 5.1software • • • Luminex Bring your biomarkers tolife with Specifications xMAP results, whichinstrumentascientist usesfortheirresearch. nomatter tofitthecapabilitiesof each platform. This software adapts enable you togainmore informationquicklyandreliably. software. Ourongoingdedicationandindustry-leading experiencewithmultiplextechnology available, ofmultiplexanalytes portfolio weprovide you themaximumpowerofLuminex Enhance your generationwithourLuminex data Driving Assay Consistency As aLuminex technology, themosttrusted,widelyusedplatformforbiomarker screening andproteinanalysis. LIS Security Automation CFR 21Part 11 Weight (Analyzer) (W xDH) Dimensions includingPC Dynamic Range Bead Compatibility Multiplex Capacity Applications Hardware Description Read Time(MicrotiterPlate) Instrument Optics Software Cat. No.Cat. and fasterwashingtechniques, includinghigh- better toamagneticfield,enabling and efficiently Magnetic bead-basedformat:Responds rapidly sample usingamaximumof25µLperwell. time, screen more inasingle than40analytes Low samplevolume: Withminimalhands-on parameters. toexplore sets multiple to constructcleanerdata less pronetousererror, enablingtheend-user is generatedsimultaneously Multiplexing: Data ® technology offers many advantagescompared tootherimmunoassay technologyoffers methods: ® partner, weare apreferred distributorofLuminex ® ® xPONENT systems andMILLIPLEX Magnetic andnonmagnetic The fastest,mostflexible ~75 min/384-wellplate FLEXMAP 3D ~20 min/96-wellplate Flow Cytometry-based Protein/Nucleic Acid Lasers/APDs/PMTs 110 x6263cm 77.1 kg (170lbs) xPONENT ® 4.2 data acquisitionplatformproviding 4.2data aconsistentexperience, inputto fromdata platform. 4.5 logs 40-014 500 3 3 3 3 ® ® System 4.2 ® instrumentsolutions. Combinedwiththelargest used Luminex • • Gen 2oftheoriginalwidely Magnetic andnonmagnetic Luminex so criticaltoyour work. faster, gainingtheearlyandcomprehensive data 384-well format,enablingyou to doyour work different inasinglesample, analytes in96-or measure theconcentrationofalargenumber Speed/high-throughput: Simultaneously that solid-phasedflatarrays cannotprovide. xMAP Reproducibility: High-volume productionof throughput washingoptions. ~40 min/96-wellplate Flow Cytometry-based 100 (80forMagPlex Protein/Nucleic Acid Lasers/APDs/PMTs 115 x6050cm 49 kg (113lbs) xPONENT ® microspheres allowsassay standardization 3.5 logs ® 40-012 ® 200™System instruments, accessoriesand ® 3 3 3 3 100™System. ® 4.2 ® ) effective modelforrunning The newestmostcost- ~60 min/96-wellplate Protein/Nucleic Acid Fluorescent Imager MAGPIX 17.5 kg (38.5lbs) LED/CCD Camera 70 x6050cm ® protein assays. xPONENT xMAP Magnetic 3.5 logs 40-072 50 3 3 3 3 ® System ® ®

4.2 ®

INSTRUMENTS

LUMINEX® SYSTEMS For Research Use Only. ® Cat. No. Cat. No. Cat. No. 40-50015 40-50018 SVCLUMPLT MPXDF-4PK SVCLUMSLV SVCLUMBRZ Protocol No. SVCLUMGLD VP-FM3D-4.2 LX2-CAL-K25 Coming Soon! F3D-CAL-K25 MPX-CAL-K25 VP-MAGPIX-4.2 LX2-PVER-K25 F3D-PVER-K25 MPX-PVER-K25 SVCLUMPLT360 SVCLUMGLD360 92-00040-00-002 SVCLUMPLTFM3D SVCLUMSLVFM3D SVCLUMBRZFM3D SVCLUMGLDFM3D SVCMAGPIXPLATPM SVCLUMGLDMAGPIX SVCMAGPIXGOLDPM SVCLUMPLT360FM3D SVCLUMGLD360FM3D 1 L 4.2 platform 20 L 4.2 platform • • • • • ® ® | 21 MerckMillipore.com/luminex 25 uses 25 uses 25 uses 25 uses 25 uses 25 uses 2 (PM) 200™ and required for FLEXMAP 3D 200™ and required Pack Size Pack ® 4.2 platform assay support see TRONSITE). map assay ® ® engineers the use of tailored to ® 4 pack, 750 mL ea for xPONENT ® Systems Installation Systems Qualification • • • • • • • • • ® 200™ xPONENT xPONENT ® 1 (PM) ® IQ/OQ Protocol FLEXMAP 3D Field Service** MAGPIX Luminex only (for MILLIPLEX ** Optional for Luminex Luminex (IQ) and Operational Qualification (OQ) ®

Repair Unlimited Emergency ) ® Systems Systems ® Cat. No. Cat. No. • • • • • • • • • • • • • • • TRONSITE-MA TRONSITE-MPX Remote Remote Support ) TRONSITE-FM3D Unlimited TRONSITE-LX200 ® instruments, we supply full service and throughout at point of sale instruments, TRONSITE-BIOTEK TRONSITE-MAGPIX ® response Onsite 2-business days 100/200™ & FLEXMAP 3D ® System Onsite System Calibration Kit Kit Verification Performance , Gold , Gold 360* , Platinum , Platinum 360* , Bronze , Silver ® ® ® ® ® ® ® ® ® Analyst 5.1 Software 5.1 Software Analyst map Kits Training ® ® System System 200™ System Onsite 200™ System 200™ Calibration Kit (xPONENT Kit (xPONENT Verification 200™ Performance 200™, Gold 200™, Gold 360* 200™, Platinum 200™, Platinum 360* 200™, Bronze 200™, Silver System Onsite Installation System Drive Fluid Drive Calibration Kit Kit Verification Performance , Gold , Platinum , Standard ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® Washer Onsite Installation Washer ® 1 Year Warranty Plans Warranty 1 Year MAGPIX FLEXMAP 3D Luminex Luminex Description MAGPIX Description Luminex MILLIPLEX and Training and Training Luminex Installation and Training FLEXMAP 3D Luminex Onsite Installation and Training BioTek MILLIPLEX Installation and Training FLEXMAP 3D FLEXMAP 3D FLEXMAP 3D Luminex Luminex Luminex MAGPIX MAGPIX FLEXMAP 3D Luminex FLEXMAP 3D Sheath Fluid (20x concentrate) FLEXMAP 3D MAGPIX Sheath Fluid for Luminex Luminex MAGPIX FLEXMAP 3D MAGPIX Onsite 1-business day response response Onsite 1-business day 1 (PM) = includes 1 preventive maintenance 2 (PM) = includes 2 preventive maintenances includes 2 preventive maintenance 2 (PM) = 1 (PM) = includes 1 preventive Luminex by developed * 360 service includes on-site support for assays Training Consumables Service Contracts As a preferred partner of Luminex As a preferred Instrument and Kit Service, Training and Consumables Service, Kit and Instrument Training your research. Multi-level service contracts provide cover from Luminex from service cover contracts provide Multi-level research. your your new instrument. Our staff can help you install the instrument, train you to use your platform, andyour to use you you install Our staff new instrument. train the instrument, can help your to run it as required. with the appropriate reagents you continue to supply INSTRUMENTS

For Research Use Only. MILLIPLEX® ANALYST 5.1 SOFTWARE 22 |MerckMillipore.com/luminex In developing thecurve-fitting algorithmsforMILLIPLEX analysisdata packages. curves. andarestandard intheseregions commonlymissedby Data canbeimportant existingmultiplex MILLIPLEX validated usingreal-life sets data from ourexperienceinassay manufacturingand algorithm,developedUse ouradvanced curve-fitting MILLIPLEX low andhighendsofthecurves, andthatworkwelleven curves oflowquality. withstandard curves todeterminethe fitthatwouldgive standard CVs thelowest usingactualexperimental sets atthe and TNFα). Curves generatedusing5parameterlogarithmiccurve fitusingMILLIPLEX Figure 6.Example fromtheMILLIPLEX data foruseinpresentationsandrecord completemultiplexdata keeping.Easily export MILLIPLEX minimum detectable concentration. minimum detectable Table fromHSTCMAG-28SK curve performance 1.Standard IL-6 analytes andTNFα using MILLIPLEX DC =(0.047,2025) =0.16%,R²0.9999 Chi =1.88%,CV 5-P.log(2.96,Curve statistics: 11.89,0.52,3.19,0.31) MILLIPLEX MILLIPLEX MILLIPLEX Standard MILLIPLEX Description Blank IL-6 Std 1 Std 2 Std 3 Std 4 Std 5 Std 6 Std 7 Blank TNFα Std 1 Std 2 Std 3 Std 4 Std 5 Std 6 Std 7 ® ® ® ® ® Analyst Edition –5seatlicense 5.1Software Database Analyst Edition –1seatlicense 5.1Software Database Analyst 5.1Software –5seatlicense Analyst 5.1Software –1seatlicense Analyst derived 5.1software wasdesignedtofocusondata fromthelowandhighendsof Exp pg/mL ® Analyst Reports 5.1Detailed 109.38 11.72 46.88 187.5 27.34 437.5 1750 0.18 0.73 2.93 0.43 1.71 6.84 750 0 0 ® Analyst 5.1Software 18348 10648 23262 ® 211.5 22.25 246.5 954.5 DC =(0.097,7853) =0.14%,R²0.9999 Chi =1.66%,CV 5-P.log(2.13,Curve statistics: 11.10,0.41,3.06,0.47) 3052 8262 3665 Human High Sensitivity TCellpanel(HSTCMAG-28SK, HumanHighSensitivity IL-6 analytes 38.5 83.5 MFI 847 24 75 36 Obs pg/mL 181.59 759.68 111.88 428.21 11.99 27.45 1758 0.18 0.74 2.86 47.5 0.43 1.74 6.66 — — ® Analyst 5.1,simulationswere runon600+data % CV 11.79 11.31 11.12 5.51 5.68 9.18 4.73 5.02 1.92 3.93 2.54 3.73 4.96 3.43 0.05 1.01 ® Analyst 5.1. with expectedconcentrations. observed concentrations between distance Chi –TheChi-Squared ofthe teststatistic curve replicates ateachdilutionlevel. –TheCoefficientofCV standard Variationof concentrationrange.DC –Thedetectable Notes: % Recovery ® Analyst 5.1.minDC= 100.69 102.34 101.33 101.29 101.83 100.38 102.29 100.44 99.99 97.64 96.85 99.62 97.42 98.11 — — minDC pg/mL Cat. No.Cat. 40-089 40-088 40-087 40-086 0.047 0.097 WASHING SOLUTIONS For Research Use Only.

40-094 40-095 40-096 40-097 Cat. No. map

® 405™ LS ® washers come pre- ® | 23 MerckMillipore.com/biotek 405™ TS models have a state-of-the-art, high- a state-of-the-art, 405™ TS models have user interface LED backlit touch screen resolution and flexible instrument for an intuitive 405™ Ultrasonic TS models come with the built-in with the cleaning even enabling easy Advantage™ toughest of sample types • •

405™ Select TS (touch screen) Washer (left); the BioTek Washer 405™ Select TS (touch screen) ® -approved ® xMAP ® , we offer the latest advancements in 96- and 384-well plate washing: a fully in 96- and advancements , we offer the latest ®

plate washer models: the BioTek ® map and Luminex ® washer advantages: washer advantages: ® 405™ LS Magnetic 96-well Washer Filtration 96-well Washer 405™ LS Magnetic/Vacuum Cleaning and Ultrasonic Screen Complete with Touch 405™ TS Magnetic 96-well Washer and Ultrasonic Cleaning Screen Complete with Touch Filtration 96-well Washer 405™ TS Magnetic/Vacuum ® ® ® ®

MILLIPLEX Fast and hands-free full plate washing and hands-free Fast High-energy neodymium iron boron magnets for rapid separation of multiplex beads withfor rapid separation of multiplex beads superior retention Description BioTek BioTek BioTek BioTek Washer (right). Washer Figure 7. BioTek Figure • BioTek •

In partnership with BioTek Washing solutions for MILLIPLEX for solutions Washing automated system designed to quickly wash an entire plate through biomagnetic separation, washing and plate through biomagnetic designed to quickly wash an entire automated system the 405™ vacuum filtration options—with allow magnetic and systems which We offer two filtration. vacuum These newest BioTek screen. and glove-touchable an easy-to-use TS model offering loaded with our validated wash protocols. loaded with our validated

• and all your plate-based assays plate-based all your and For Research Use Only. WASHING SOLUTIONS 24 |MerckMillipore.com/biotek • • • • securely heldtothesidesby surroundingeachwell. 9magnets offthe and blotting remainder onapapertowel.Magneticbeadsare plate toberemoved by simplydecantingor“flicking” thecontents ofthe96-well handheld magneticseparatorallowstheliquidcontents map magneticimmunoassays withoutlossinassay performance. The • • • Magnetic BeadAssays • • • • Hands-free washingwithasmallfootprint Reagent Dispenser Better together:ELx50washerwithMILLIPLEX Better BioTek instrumentforMILLIPLEX The perfect BioTek We offeralow-costalternative toautomatedwashingofMILLIPLEX Well orConical Flat Bottom Plates Handheld MagneticSeparatorBlockfor96-­ Conical Well Plates or Handheld MagneticSeparatorBlockfor96-wellFlatBottom Description BioTek Description BioTek Description Magnetic strength: (MGO) 52MegaGaussOersteds grippingforallsizesofhands O-Ring onbaseplatefacilitates separator block clipsystemAdjustable holdsawidevariety ofmicroplatestothe base resistant steelplateunderneath,allmountedtoapolypropylene Top magneticframeiswhitepolycarbonate, withacorrosion- assay plates delivery—ensuring completecontrolwhile washingmultiplex Self-contained, programmablewasherenables precise fluidic assay plates Optimized magnetforstripwellsandflat-bottom magneticbead Reduced hands-on timeformultiplexassays 384-well assay plates delivery—ensuring completecontrolwhilewashing96-welland Self-contained, programmablewasherenablesprecise fluidic ofalargerrobotforfractionthecost The performance Optimized forboth384-and96-wellplates walk away Fully automatedprocess:loadstandards, andsamples QCs ® ® ELx50Magnetic96-wellStripWasher MultiFlo™ FXAutomatedReagent Dispenser ® ® Magnetic96-wellStripWasher — MultiFlo™ FXAutomated ® map384-wellAssays ® map

well Cat. No.Cat. Cat. No.Cat. Cat. No.Cat. 40-099 40-285 40-062

®

384-WELL OPTIONS For Research Use Only. ®

®

Analyst Software Software Analyst ® FLEXMAP 3D 4.2 software ® ® | 25 MerckMillipore.com/milliplex

(Cat. No. 40-014) (Cat. No. 384-well capable system with384-well capable system xPONENT representation 7. Side-by-side Figure of min DCs in the 384-well and the of 18 analytes determined 96-well assay MILLIPLEX by (384-well, n=12; 96-well, n=3). Luminex

TNFα

β β MIP1 IL-8

96-well IL-7

IL-6

IL-5

IL-4

384-well

1 MultiFlo™ FX, and the 384-well reading capability of the FX, and the 384-well reading MultiFlo™ IL-2 Sensy MultiFlo™ FX MultiFlo™

®

® IL-2 e

brand. Extensive testing for standard curve range, sensitivity, sensitivity, testing for standard range, map brand. Extensive curve IL-1β ®

Fully automated reagent dispenser automated reagent Fully performance superior provides option and walkaway 40-099) (Cat. No. BioTek naly IL-17A

3 IL-1 , users can increase their throughput to ~22,932 data their throughput , users can increase points in a

®

0 IL12p7

α MIP3

increases your throughput and decreases your hands-on time compared to time compared hands-on your throughput and decreases your increases ® IL-10 Coarae ssay Human High Sensitivity T Cell 21-plex assay for 384-well plates, BioTek for 384-well plates, map Human High Sensitivity T Cell 21-plex assay

® IFNγ 384-well Human High Sensitivity T Cell Panel is analytically validated to deliver the to deliver analytically is validated Sensitivity map 384-well Human High T Cell Panel GM-CSF ®

map Kits ® ITAC map 384-well Human

® FLEXMAP 3D ®

3.00 2.50 2.00 1.50 1.00 0.50 0.00

n DC DC n L MultiFlo™ FX and FLEXMAP 3D MultiFlo™ High Sensitivity T Cell Panel High Sensitivity T Cell Panel (Cat. No. HSTCMAG384-PX21) (Cat. No. With the MILLIPLEX The Data MILLIPLEX The Tools The MILLIPLEX Using 3 Plates Using 22,932 Data Points in 24 Hours 24 in Data22,932 Points MILLIPLEX quality you expect from the MILLIPLEX quality you equivalent 96-well protocols. equivalent single day (based on 3 assays per day). (based on 3 assays single day This kit, plus reagent addition via the BioTek addition This kit, plus reagent precision, cross-reactivity and recovery shows that the 384-well format produces comparable, if not comparable, that the 384-well format produces shows and recovery cross-reactivity precision, to the 96-well format kit. results superior, Luminex For Research Use Only. SINGLE PROTEIN DETECTION 26 |MerckMillipore.com/immunoassays • • • HT assays are unique, providing: our ELISAs, RIAsandGyroMark™ needs, includinghighthroughput, For othersingleproteindetection systems. • • • needs withour: your singleproteindetection Choose theplatformthatmeets use withSMCxPRO™ orErenna own SMC™immunoassays for tobuildyour optimization kits well asassay development and a rangeofimmunoassay as kits biomarkers iscritical.We offer detecting lowlevels ofprotein measurements when sensitivity and technologyallowforhigh Our SMC™immunoassay kits tomeetyourproducts specificneeds. Measuring singleproteinbiomarkers? We offerarangeof Single ProteinAnalysis Solutions In-assay controls plasma conditionsinserumor analyte simulate conditionsofnative curves thataccurately standard Serum matrixforgenerating reference lots validatedStandards tomatch GyroMark™ HTkits ELISA/RIA kits SMC™ immunoassay kits

®

reference lot). of (±10% lots 16 past the for ELISA (EZHPI-15K)values Proinsulin fortheHumanIntact show consistent values control quality high and Figure 8.Low control testing. and usedforfinalquality arestandards available World HealthOrganization pM 50.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 0.0 5.0 1 2 Human IntactProinsulinELISA 3 Quality ControlV 4 5 6 QC2 7 alues forPast16Lots 8 9 QC1 10 11 Kit 12 13 14 15 16 SINGLE PROTEIN

SINGLE PROTEIN DETECTION: SMC™ For Research Use Only.

Quantify laser; detected is by Sample detection counted tags 5 | 27 MerckMillipore.com/smctech PROPRIETARY

Elute chemically is Complex broken apart 4 Detect 3

instrument, eluate is then drawn into the system capillary tube, the system instrument, eluate is then drawn into ® Capture on Plate or Bead or Plate instruments capture the sum of all digital events counted above a counted above the sum of all digital instruments events capture ® 2 STANDARD Technology ™ instrument, a proprietary as an algorithm computes the total sum of all photons recorded ® Analyte of Interest in Solution

1

SMC™ technology provides maximum immunoassay performance similar while following a workflow immunoassay maximum provides SMC™ technology step and elution a unique assay combining By as shown below. technology, to traditional ELISA traditional over signal-to-noise ratios improved robust digital achieves counting, SMC™ technology quantification at both low enhanced The SMC™ technology thus provides technologies. immunoassay system. on one complete of expression and high levels Single Molecule Counting (SMC™) Technology: Molecule CountingSingle Technology: (SMC™) signal increased + background Reduced additional standard curve. The SMCxPRO™ instrument digital event algorithm computes digital algorithm computes events instrument digital event The SMCxPRO™ standardadditional curve. sensitivity and assay thus improving across a spectrum of time series for a single standard curve, with traditional technologies. can be achieved what extending the dynamic measuring range beyond background threshold. This model can have limited dynamic range, so at higher concentrations on the so limited dynamic range, This model can have threshold. background Erenna Digital counting improves sensitivity and dynamic range Digital counting improves and Erenna The SMCxPRO™

SMC™ Assay Workflow SMC™ Assay into a signal. antibodies translate each biomarker specific and detection steps, During the capture are dissociated from detection antibodies dye-labeled fluorescent During the modified elution step, the immunocomplex. In the Erenna SMC which contains a small interrogation space that is illuminated by a laser. Single fluorescently labeled Single fluorescently laser. a which contains interrogation space that is illuminated by a small space as detected events. pass through the interrogation the laser as they by excited molecules are The generation instrument supporting is the second assays. SMC™ technology The SMCxPRO™ collects technology approach signal from analyte fluorochromes free-floating in SMCxPRO™ counted by and individual photons are camera optics, CCD suspension using a rotating laser, Photodiode (APD). Avalanche SINGLE PROTEIN

For Research Use Only. SINGLE PROTEIN DETECTION: SMC™ 28 |MerckMillipore.com/smcxpro • • Min. InstrumentPerformance Specifications • SMCxPRO 2 1 • • • Instrument Specifications • • Assay Format • Read PlateFormat • RequirementNetwork/PC Specifications Response measurements from30fMcalibrator, n=20  Dynamic Range Precision Limit ofDetection(LoD) Background Slope Metric with fluorophore Determined fromcalibratorset:0-300 fMof150kDantibodylabeled (32- or64-bitcompliant) xPRO OperatingandAnalysis Software included server IPaddressA static andanFTP (Operating range180-250V) International: 230VAC, 50-60Hz (Operating range90-125V) US: 115VAC, 50-60Hz Weight: 22.7kg (50lbs) W x444.5mmD(16"H14"W17.5"D) Instrument dimensions:406.4mmHx355.6 Plate-based assays Bead-based assays 384-well plate Microsoft Windows ® 7/10OperatingSystems

™ Immunoassay System

>1.2 response/fM <3 response/fM Specification <10% CV >4 logs <1 fM 2 1 1 sales specialistformore information. • • • andTrainingInstallation SMCxPRO™ andErenna develop your ownimmunoassays foruseonthe This trainingwillprovide thetoolsyou needto your oratoneofourglobaltrainingcenters. facility based assay format.We offer hands-on trainingat specific immunoassays ineither bead-orplate- technology whiledeveloping your own application- Take advantage ofultrasensitive (SMC™) digital Advanced CustomerTraining • • • forSMCxPRO™ Platform Support Three Levels ofOnsiteService Jitterbug PlateShakerJitterbug Tecan Hydroflex Microplate8-Channel Washer MAG plate BioTek SMCxPRO™ PQService SMCxPRO™ IQ/OQInstallation SMCxPRO™ System Training – 1 day SMCxPRO™Standard Installation SMCxPRO™ CompleteSystem SMCxPRO™ ProductServices &Ancillary SMC™ Assay Development Training day -extra SMC™ Assay Development Training -3days Description Optional IQ/OQ and PQ Services availableOptional IQ/OQandPQServices performance Run ofASSIST programtoverify instrument of unit Set upofunitonbenchandcompletecalibration maintenance visitforoneyear LaborforonepreventativeEssential Service: visit foroneyear one preventative maintenanceandonefloating Labor,Advanced Service: travel andexpensesfor one year travel, forany for service expensesandallparts Total includinglabor, Unlimitedvisits Service: ® 405Touch MicroplateWasher with ® instruments. Askyour 70-0009-00 95-0014-00 95-0004-05 96-2013-00 96-2012-00 96-2022-00 96-2011-00 95-0100-00 96-2025-00 96-2024-00 Cat. No.Cat. Cat. No.Cat. SINGLE PROTEIN DETECTION: SMC™ For Research Use Only. Cat. No. 95-0017-00 96-0016-00 95-0004-05 95-0014-00 70-0009-00 70-0018-00 96-0015-00 96-0032-02 Instrument ® | 29 MerckMillipore.com/smctech Installation ® with Sgx Link ® & Ancillary Product Services Immunoassay System & Accessories System Immunoassay with Sgx link IQ/OQ Installation 405 Touch Microplate washer with Microplate washer 405 Touch ® ® ® ® Unlimited visits including labor, Service: Unlimited visits including labor, Total expenses and all parts service for for any travel, one year and expenses for travel Service:Advanced Labor, and one floating maintenance one preventative visit for one year Essential Service: Labor for one preventative maintenance visit for one year Erenna Complete Erenna PQ for Erenna MAG plate MAG Washer 8-Channel Microplate Hydroflex Tecan Jitterbug Plate Shaker Heat Sealer Erenna BioTek Erenna Three Levels of Onsite Service of Onsite Levels Three Supportfor the Erenna • • • † <1 fM* >4 logs <100 DE <7% CV

>20 DE/fM* Specification

Immunoassay System System Immunoassay 7 Operating Systems ® ®

Int.: 230 VAC, 50-60 Hz Int.: 230 VAC, U.S.: 115 VAC, 50-60 Hz 115 VAC, U.S.: (operating range 90-125 V) (operating range 180-250 V) Width: 540 mm (21.25 in) Depth: 575.6 mm (22.7 in) (69 lbs) 31.3 kg Weight: Height: 400 mm (15.75 in) Microsoft Windows A static IP address and an FTPA static IP address server software Sgx link Operating and Analysis included Determined from calibrator set: 0–300 fM of 150 kD antibody labeled with fluorophore Dynamic Range Slope Background Limit of detection (LoD) Precision Metric DE measurements from 30 fM calibrator, n=20 from 30 fM calibrator, DE measurements † *  • Specifications Min. Instrument Performance Power Requirement Power • • • • Instrument Dimensions and Weight Instrument Dimensions and • • Network/PC Requirement Erenna

• • Specifications SMC™ Bead-based Immunoassay Kits

Median LLoQ Endogenous Sample Analyte (pg/mL)1 (pg/mL)2 Species3 Type4 Catalog No. Akt1 (Ser473) 0.98 NA H, M, R L 03-0100-01 Akt1 (total) 7.8 NA H, M, R L 03-0099-01 Amyloid beta 1-40 Coming Soon H, M, R C, P 03-0145-00

SINGLE PROTEIN DETECTION: SMC™ SINGLE PROTEIN Amyloid beta 1-42 Coming Soon H, M, R C, P 03-0146-00 cTnI 0.69 1.75 H, Cy, R, C, GP P, S 03-0092-00 G-CSF 0.08 17 H P 03-0047-00 GM-CSF 0.02 0.2 H P 03-0067-00 GLP-1 (active) 0.4 3.46 H, M, R, C P 03-0024-03 GLP-1 (total) 0.39 17.8 H, M, R, C P 03-0025-06 IFN-γ 0.2 0.79 H P 03-0049-00 IL-1α 0.39 1.06 H P 03-0072-00 IL-1β 0.2 P: 0.45; S: 1.08 H P 03-0028-00 IL-2 0.05 0.21 H P, S 03-0051-00 IL-4 0.04 0.12 H P 03-0052-00 IL-5 3.91 4.52 H P 03-0053-00 IL-6 0.08 1.3 H P, S 03-0089-01 IL-7 0.39 4.91 H P, S 03-0094-00 IL-8 / CXCL8 0.24 3.6 H P, S 03-0055-00 IL-10 0.39 1.01 H P 03-0056-00 IL-12 0.05 0.13 H P, S 03-0057-00 IL-13 v2 0.04 0.21 H P, S 03-0109-02 IL-15 0.1 3.38 H P, S 03-0058-00 IL-17A v2 0.03 0.12 H P, S 03-0103-00 IL-17F v2 0.2 0.86 H P, S 03-0102-00 IL-17A/F Heterodimer v2 1.2 2.75 H P, S 03-0119-00 IL-21 0.2 0.53 H S 03-0014-07 IL-22 0.2 3.3 H P 03-0059-01 IL-23 0.1 0.18 H P, S 03-0112-00 KIM-15 3.91 P: 65; S: 75; H P, S, U 03-0118-00 U: 147 TNFα 0.2 2.3 H P 03-0088-00 TNFα 0.4 38.6 M S 03-0108-00 VEGF 0.2 66.5 H P 03-0068-00

1 LLOQ: Lowest point on standard curve with CV < 20% and accuracy within 20% of expected values. 2 Median Endogenous: Minimum of 10 samples from individual donors assessed for ability to quantify baseline biomarker values. 3 Optimized for first species type listed. Other listed species have been tested, but not optimized for peak performance. Key: H=Human; M=Mouse; R=Rat; GP=Guinea Pig; Cy=Cynomolgus Monkey; C=Canine 4 Optimized for use in sample type(s) listed. Key: P=Plasma; S=Serum; L=Lysate; U=Urine; C=Cerebrospinal Fluid (CSF) 5 KIM-1 is a plate-based assay. For Research Use Only. Research For

30 | MerckMillipore.com/smctech SINGLE PROTEIN DETECTION: SMC™ For Research Use Only. 03-0137-00 03-0127-00 03-0126-00 03-0125-01 03-0123-00 03-0133-00 03-0134-00 03-0132-00 03-0136-00 Catalog No. | 31 MerckMillipore.com/smctech Serum Serum Serum Serum Serum Serum Serum Serum Serum Sample Type Assays within 20% of within Dilutional linearity: Assays expected value from Minimum of 10 samples Endogenous range: individual donors assessed for ability to quantify levels biomarker • • 4.9 6.2 100 0.49 0.49 0.98 3.91 0.24 0.49 LLoQ LLoQ (pg/mL) platform ®

Low-cost Reproducible LLOQ Simple workflow Minimal sample volume Samples run precision: and intra-assay Inter- and Spiked multiple days. over on multiple plates samples within 20% across experiments un-spiked spiked Minimum of 10 samples recovery: Spike between 80–120% with acceptable recovery Lower limit of quantitation: Lowest point onLower standardwith CV within<20% and accuracy curve 20% of expected values Mouse TNFα Mouse IL-22 Mouse IL-21 Mouse IL-17F Mouse IL-17A Mouse IL-17A Mouse IL-13 Mouse IL-13 Mouse IL-10 Mouse IL-10 Mouse IL-5 Mouse IL-5 Analyte Mouse IL-4 Mouse IL-4

Verified Immunoassays Verified Validation Criteria for for Criteria Validation • • • • Immunoassay Kits Immunoassay complete kits as provided are to immunoassays Discovery Erenna run on your Mouse Plate-based Discovery Discovery Mouse Plate-based • • • A comprehensive set of criteria evaluating ultimate quantitative ultimate quantitative of criteria evaluating set A comprehensive performance used to qualify are immunoassays verified Expert Custom Services & Sample Testing for SMC™ Technology

Custom services and sample testing when time and resources require an expert outsource partner to help accelerate programs from discovery into clinical trials.

SINGLE PROTEIN DETECTION: SMC™ SINGLE PROTEIN Our capabilities include:

Collaborative Biomarker Discovery & SMC™ Assay Kit Development Development • Proprietary or commercial SMC™ assay kit • Fit-for-purpose sample testing (non-GLP) development • Immunoassay development • Assay kit verification and validation • Biomarker validation (to sponsor requirements) • Assay kit manufacturing • Method transfer to CRO Biomarker Assay Services for Biotherapeutic Discovery Sample Testing • Pharmacokinetics • R&D and prototype assay kits • Pharmacodynamics • Commercial assay kits • Immunogenicity • Potential biological relevance in real samples Antibody Derivatization/Labeling and • Biological anomalies that may occur due to drug Characterization interference, matrix effects, etc. • Antibody screening and selection • Antibody labeling • Assay stability testing For Research Use Only. Research For

32 | MerckMillipore.com/smctech SINGLE PROTEIN DETECTION: ELISA For Research Use Only. Cat. No.* EZHI-14BK EZRL-83BK EZRL-83BK EZHL-80BK EGLP-35BK EZHR-95BK EZML-82BK EZHA-52BK EZHPI-15BK EZCCP-47BK EZHPP-40BK EZGRT-89BK EZRMI-13BK EZGLU-30BK EZHCP-20BK EZGRA-88BK EZHGIP-54BK EZHADP-61BK EZMADP-60BK EZRMGH-45BK EZGLP1T-36BK Bulk Packaging EZGLPHS-35BK EZRMGIP-55BK EZHFGF23-32BK Cat. No. EGLP-35K EZHA-52K EZHI-14K EZCL-31K EZRL-83K EZHR-95K EZCCP-47K EZML-82K EZGRT-89K EZHCP-20K EZGRA-88K EZHPI-15K EZHL-80SK EZHPP-40K EZRMI-13K EZGLU-30K EZGLP2-37K EZHGIP-54K EZRGRT-91K EZRADP-62K EZHADP-61K EZRGRA-90K EZMADP-60K EZGLP1T-36K EZGLPHS-35K EZHPYYT66K EZRMGIP-55K EZRMCP2-21K EZRMGH-45K EZHIASF-14K EZHFGF21-19K EZHFGF23-32K EZHMWAN-65K EZHMWAN-65K EZMSAA3-12K EZPIIANP-53K | 33 MerckMillipore.com/elisa_ria EZRMFGF21-26K

5 µL 20 μL 10 μL 20 μL 20 μL 10 μL 10 μL 20 µL 10 µL 10 µL 25 µL 50 µL 20 µL 10 µL 20 µL 10 µL 10 µL 10 µL 50 µL 10 µL 25 µL 20 µL 50 µL 10 µL 50 µL 20 µL 20 µL 20 µL 20 µL 20 µL 10 µL 50 μL 50 µL 100 µL Sample Volume 20-50 μL 150-300 μL 0.5 pM 0.7 pM 1.0 pM 1.0 pM 15.0 pM 0.14 pM 6.5 pg/mL 0.1 ng/mL 0.2 ng/mL 0.2 ng/mL 3.5 pg/mL 0.5 ng/mL 0.2 ng/mL 8.0 pg/mL 0.4 ng/mL 4.2 pg/mL 4.2 pg/mL 0.3 ng/mL 1.0 μU/mL Sensitivity 12.3 pg/mL 10.0 pg/mL 10.0 pg/mL 0.21 ng/mL 0.05 ng/mL 0.08 ng/mL 0.07 ng/mL 30.0 ng/mL 0.02 ng/mL 15.0 pg/mL 50.0 pg/mL 0.05 ng/mL 0.24 ng/mL 0.04 ng/mL 0.85 μU/mL 0.003 ng/mL 0.078 μg/mL

Standard 2–200 pM 1–100 pM 2–100 pM 1–50 ng/mL 1–64 ng/mL 25–1600 pM 4.1–1000 pM Curve Range 2–200 µU/mL 2–200 µU/mL lot dependent 0.2–10 ng/mL 0.2–30 ng/mL 0.2–30 ng/mL 0.02–2 ng/mL 0.7–50 ng/mL 0.16–5 ng/mL 0.2–20 ng/mL 0.2–10 ng/mL 0.1–10 ng/mL See data sheet 0.078-5 μg/mL 0.78–50 ng/mL 0.5–100 ng/mL 1.5–200 ng/mL 10–2000 pg/mL 25–2000 pg/mL 25–2000 pg/mL 1.56–200 ng/mL 9.9–2400 pg/mL 8.2–2000 pg/mL 8.2–2000 pg/mL 100–5000 pg/mL 12.6–3000 pg/mL 3.125–200 ng/mL 31.25–2000 pg/mL 49.4–12,000 pg/mL Species Human Human Mouse, Rat Mouse, Human Canine Mouse Rat Human Human, Rat Mouse, Human Mouse, Rat Mouse, Human Human Human Mouse Human Human Mouse Rat Human Human Canine Mouse, Rat Mouse, Human Mouse, Rat Mouse, Human Human Mouse, Rat Mouse, Human Mouse, Rat Mouse, Human Mouse, Rat Mouse, Multi-Species Multi-Species Multi-Species Multi-Species WHO WHO WHO

kit equivalent) space (10 friendly and saves environmentally on select kits — more available Bulk packaging (active) for measuring GLP-1 assay Preferred Chemiluminescent assay

WHO Description Insulin Insulin (Animal serum free) Leptin Leptin Leptin Leptin “Dual Range” Pancreatic Polypeptide Proinsulin (total) Growth (GH) Hormone IIA Procollagen Type N-Propeptide (PIIANP) (total)PYY Resistin SAA-3 Insulin Adiponectin Adiponectin (High Molecular Weight) Adiponectin Adiponectin Amylin (active) Amylin C-Peptide C-Peptide C-Peptide 2 C-Peptide FGF-21 FGF-21 FGF-23 Ghrelin (active) Ghrelin Ghrelin (active) Ghrelin Ghrelin (total) Ghrelin Ghrelin (total) Ghrelin GIP (total) GIP (total) GLP-1 (active) GLP-1 GLP-1 High Sensitivity GLP-1 ∆•• (active) GLP-1 (total) GLP-1 GLP-2 Glucagon ∆

Metabolic/Endocrine ELISAs metabolic/endocrineOur broad range of of action, aid in can help elucidate therapeutic mechanisms ELISAs metabolicmore. diseases and investigate toxicities, predict states, early diagnosis of disease ELISA and RIA KitsRIA and ELISA * •• ∆ For Research Use Only. SINGLE PROTEIN DETECTION: ELISA 34 |MerckMillipore.com/elisa_ria quantify solublebiomarkersquantify inseraandlysates. with reliable quantificationofbiomarkers. Trust ourneuropeptideandneurodegenerative ELISAstoprecisely Get acompletepicture ofthenervous system ofthecomplexitiesassociatedwith normalanddiseasestates Neuroscience: Neuropeptide&Neurodegenerative ELISAs S100B Amyloid Set beta, Amyloid 1-42 beta Amyloid 1-40 beta α-Synuclein Amyloid (Brain)1-40 beta Description NGF (Nerve Growth Factor)NGF (Nerve Growth Acidic Protein) GFAP (GlialFibrillary Neurotrophic Factor) BDNF (Brain-Derived Amyloid (Brain),Set beta Amyloid (Brain)1-42 beta NPY (NeuropeptideY) NPY (NeuropeptideY) Derived Factor) PEDF (PigmentEpithelium- (pNF-H) Sandwich Neurofilament, Phosphorylated

Human Human Human Human Human, Mouse, Rat Human Species Mouse, Rat Human, Mouse, Rat Human, Rat Human Human Human Mouse, Rat Human Multi-Species 0.0293–15 ng/mL 2.7-2000 pg/mL 0.9–62.5 ng/mL 10–1000 pg/mL 7.8–500 pg/mL 1.5-100 ng/mL 16–500 pg/mL 16–500 pg/mL 16–500 pg/mL 16–500 pg/mL 5-1000 pg/mL 0.01-2 ng/mL Contains 1eachofEZBRAIN40andEZBRAIN42 Contains Curve Range Curve 3–60 ng/mL Contains 1eachofEZHS40andEZHS42 Contains Standard Standard

0.0585 ng/mL 10-15 pg/mL 0.004 ng/mL Sensitivity 1.5 ng/mL 0.9 ng/mL 1.3 pg/mL 7.8 pg/mL 3 ng/mL 5 pg/mL 4 pg/mL 5 pg/mL 4 pg/mL 2 pg/mL Volume Sample 1-10 µL 100 µL 100 μL 50 µL 50 µL 50 µL 50 µL 50 µL 50 µL 50 µL 50 µL 20 µL 50 µL EZHS100B-33K EZRMNPY-27K EZBRAIN-SET EZHNPY-25K EZBRAIN42 EZBRAIN40 EZHS-SET Cat. No.Cat. EZHS42 EZHS40 CYT306 CYT304 CYT420 NS170 NS400 NS830 SINGLE PROTEIN DETECTION: RIA For Research Use Only. PI-12K RI-13K HI-14K XL-85K HL-81K GL-32K LPI-16K Cat. No. SRI-13K HPI-15K SHL-81K HCP-20K PYY-67HK CCP-24HK PYYT-66HK GHRT-89HK HADP-61HK GHRA-88HK GLP1T-36HK GLP1A-35HK RMPYY-68HK 5 µL 50 μL 50 µL 50 µL 50 µL 50 µL 50 µL 50 µL 50 µL 50 µL 50 µL 50 µL 300 µL 300 µL 100 µL Sample Volume <100 µL <100 µL 50–100 µL 100–200 µL 100 µL (20 µL)

| 35 MerckMillipore.com/elisa_ria 3 pM 3 pM 2 pM 1 ng/mL 1 ng/mL 2 μU/mL 2 µU/mL 93 pg/mL 20 pg/mL 20 pg/mL 10 pg/mL 7.8 pg/mL 0.1 ng/mL 0.1 ng/mL 0.5 ng/mL 2.5 µU/mL Sensitivity 15.6 pg/mL 0.15 ng/mL 0.02 ng/mL 0.05 ng/mL (78.1 pg/mL)

Standard 2–100 pM 10–500 pM 1–50 ng/mL 10–1000 pM 0.1–5 ng/mL Curve Range 2–200 μU/mL 2–200 µU/mL lot dependent lot dependent lot dependent lot dependent lot dependent lot dependent kits for your research needs. research map kits for your 0.1–10 ng/mL 0.02–1 ng/mL 20–400 pg/mL 0.5–100 ng/mL 0.05–10 ng/mL ® 2.5–250 µU/mL 0.156–20 ng/mL Species Human Canine Human Human Human Multi-Species Multi-Species Multi-Species Porcine Rat Multi-Species Rat Human Human Multi-Species Human Human Mouse, Rat Mouse, Human Human Adiponectin C-Peptide C-Peptide (active) Ghrelin (total) Ghrelin Description GLP-1 (active) GLP-1 (total) GLP-1 Glucagon Insulin Insulin Insulin LisPro Insulin (sensitive) Insulin Specific Leptin Leptin Leptin (sensitive) Proinsulin PYY PYY (3-36) PYY (total)PYY Radioimmunoassays (RIAs) Radioimmunoassays protein concentration. to measure accurate way economical and a valuable, been considered long RIAs have to recommend we would like material, from using radioactive away move researchers as many However, MILLIPLEX our ELISAs or consider using that you For Research Use Only. SINGLE PROTEIN DETECTION: GyroMark™ HT ASSAYS 36 |MerckMillipore.com/gyromark Visit: Fully fortheGyrolab qualifiedkits GyroMark™ HTAssays Considering owningyour ownGyrolab • • • • • nanoliter-scale microfluidicstructures. forcestosteerliquidflowinproprietary and capillary through precise, automatedcontrolofcentrifugal save time, sampleandreagents. Thisisachieved overdata abroaddynamicrange, andhelpsyou Gyros technologydelivers immunoassay highquality Automated platformforbiomarker detection. More withlesssamplevolume: data As aGyrolab reproducible assays tosave you timeandmoney! company toprovide highquality, robustand We have partneredwithGyrostobethefirst biomarker studythroughevery phase. especially whenyou along-term needtosupport your canbetime-consumingandcostly, ownkits fast thetechnologycanbe. However, developing Open platform.Full flexibility. Gyros platformwithconfidence. Move your assay fromconventional ELISAtothe Nanoliter precision. Reduced consumption. flexibility. Minimize matrixeffects. and Maximizesimplicity dilutions andrepeats. frompgtoμg.Minimize data High-quality [email protected]. To aspecialistat contact getstarted, assay ontheGyrolab Interested indeveloping your own gyros.com. ® user, you knowhowreliable and ® platform? ® workstation? workstation?

Sample volume was1,000nLandsampleswere diluted1:2. and ourMulti-Species GLP-1 Active No. ELISA(Cat. EGLP-35K). Species GLP-1 Active Assay fortheGyrolab GLP-1 theGyroMark™ Active between standards HTMulti- Figure 9.HighcorrelationExcellent toELISAdata. overlap of *MTO isMadetoOrder NGAL/Lipocalin-2 KIM-1 Insulin Insulin Infliximab ADA IL-6 IL-6 IL-33 Polypeptide (HPP) Human Pancreatic Glucagon GLP-1 (total) GLP-1 (active) Cystatin C Clusterin β-2-Microglobulin Drug Quant Adalimumab ADA and Description ® platform! Response GLP-1 ActiveGyrosvs.ELISAStandard 1000 0.01 100 0.1 10 1 0.1 ELISA Standard Concentration (pM) 1 Rat Rat Rat/Mouse Human Human Mouse Human Human Human Multi-Species Multi-Species Multi-Species Rat Rat Rat Human Species 10 GYROS Standard 100 ® xPWorkstation 1000 Status* MTO MTO MTO MTO MTO MTO MTO MTO MTO MTO MTO MTO MTO MTO MTO MTO CUSTOM

CUSTOM IMMUNOASSAYS AND SERVICES For Research Use Only.

Assay Validation xPlore™ ® systems* ® | 37 MerckMillipore.com/customassay xP and Gyrolab ® Assay Verification Please refer to the SMC™ technology section for complete to the SMC™ technology section for Please refer and sample custom assay details on how to meet your testing needs. Gyrolab GyroMark™ HT GyroMark™ Automated Single Protein Detection: * Single Molecule Counting (SMC™) Detection: Single Protein Ultrasensitive SMCxPRO™ or Erenna SMCxPRO™ Validated 200™, ® Assay Development , Luminex ® Developed and Verified (SPRCUS) and Verified Developed Prototyped (SPR) systems ® Platform ® Special Product Release (Custom) Special Product Release Reagent Reagent Characterization & Feasibility

ELISA Single Protein Detection: Plate reader compatibility FLEXMAP 3D STEP 2 validation and of verification level required type assay based on your Choose your (Prototyped, Developed and Verified or Validated). or and Verified (Prototyped, Developed Luminex Pick a platform based on the number of proteins you would like to measure in your sample. in your to measure would like on the number of proteins you Pick a platform based STEP 1 and/or throughput constraints. volume sample Consider sensitivity requirements, We offer four types of assays to meet your assay verification and validation requirements. Projects can requirements. validation verification and your assay to meet offer four types of assays We assays analytes or use of existing in a new format (including transferring include new analyte development type). for a new sample technical specialists Our consultative help will assays or verifying to a new platform solution for you. determine the right Immunoassay Development Immunoassay Fit-for-Purpose Custom Custom Fit-for-Purpose MAGPIX Multiplex Detection: CUSTOM

For Research Use Only. CUSTOM IMMUNOASSAYS AND SERVICES 38 |MerckMillipore.com/customassay for a complete list of formats availablefor acompletelistofformats by platform. Immunoassay companionpiece MenuofServices only) canbeselected.DownloadourCustom and/or premixed beads(custommultiplexassays assay (customSMC™andmultiplex) formats special bulkpackaging requirements, 384-well kitmaterials.protocol andstandard Inaddition, needs. Allcustomassays willincludeacustomized canbecustomizedtomeetyourFinal kitformats custom assay. formatforyourDetermine thekitted STEP 4 available. verification andarevalidation services also and includedforeachplatform.Additional Minimum levels ofassay verification are suggested custom assay. and validation testingneededinyour Determine theappropriatelevel ofverification STEP 3 and enables us to support youand enablesustosupport asyour research evolves. greater sensitivity. Use of the same reagents ensures consistency in epitope detection across platforms, usedinamultiplexassaytest thesameantibodypairs screen inacustomSMC™assay toachieve proprietary. We cantestantibodiesusedinany ofourassays onany platform.For example, wecan in different immunoassay formats. Antibodypairinformationincommercialimmunoassays isusually fourdifferent totestthesameantibodypair(s) One advantageofsupporting platformsistheability DID YOU KNOW? for a complimentary feasibility assessment. for acomplimentary Contact today.Get started customassay MerckMillipore.com/ for eachplatform: validation testingavailable formats, verification, and for acompletelistofassay companionpiece Services Immunoassay Menuof Download ourCustom

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Plasma Multiple Sample Sample Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Cell and Tissue Culture SupernatantsCulture | 39 MerckMillipore.com/customassay map map map map map map map map map map map map map map map map map map map map map map map map map map ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® Platform MILLIPLEX Platform HT GyroMark™ HT GyroMark™ MILLIPLEX MILLIPLEX MILLIPLEX MILLIPLEX MILLIPLEX MILLIPLEX MILLIPLEX MILLIPLEX MILLIPLEX MILLIPLEX MILLIPLEX MILLIPLEX MILLIPLEX MILLIPLEX MILLIPLEX MILLIPLEX MILLIPLEX MILLIPLEX MILLIPLEX MILLIPLEX MILLIPLEX MILLIPLEX HT GyroMark™ MILLIPLEX HT GyroMark™ HT GyroMark™ HT GyroMark™ HT GyroMark™ MILLIPLEX MILLIPLEX

Contact your local specialist or email local specialist or Contact your [email protected] analytes in the above. listed if interested BMP-9 Analyte Adalimumab ADA Adalimumab Drug QuantitationAdalimumab (BCMA) B-cell Maturation Antigen Analyte Semaphorin 3A sTM (Soluble Thrombomodulin) DKK1 Galectin-1 Galectin-3 HGFR/cMET IGF-1 Osteonectin Periostin sAXL C Tenascin TRAIL Vasopressin CSTB (Cystatin B) CSTB (Cystatin 2) of Tumorigenicity ST2 (Suppressor Vitronectin Calprotectin N CD13 Aminopeptidase Collagen 1 Elafin Galectin-1 Glucagon GRP78 Polypeptide) HPP (Human Pancreatic IL-33 IL-6 Infliximab ADA (LCAT) Lecithin–cholesterol Acyltransferase Moesin Mouse and/or Rat Human Custom Analytes Custom For Research Use Only. SOLUBLE ANALYTE APPENDIX 40 |MerckMillipore.com/immunoassays M G S E appearinalphabeticorder.Soluble analytes represents inthechart Thealphabeticletter theplatform: How tousetheappendix Appendix Soluble Analyte R Apo AI Apelin Antithrombin III ANGPTL6 ANGPTL4 ANGPTL3 Angiotensinogen (AGT) Angiostatin/Kringle Angiopoietin-2 Amyloid 1-42 beta Amyloid 1-40 beta Amylin (total) Amylin (active) Amphiregulin α-Synuclein α-MSH α-Fetoprotein (AFP) α-2-Macroglobulin (A2M) α-1-Antitrypsin (A1AT) α-1-Microglobulin α-1-Acid Glycoprotein (AGP) sALK-1 ALDH1A1 (AgRP) Agouti-Related Protein Albumin (urine) Albumin (urine) Adipsin/Factor D Weight Adiponectin, HighMolecular Adiponectin ADAM15 ADAMTS13 ACTH 5'-NT/CD73 6CKine/CCL21/Exodus-2 Analyte = = = = = MILLIPLEX GyroMark™ HTassay SMC™ (SingleMoleculeCounting)assay ELISA ELISA RIA RIA

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M ES M ES M ER M E M E M M M M M M M M M M M M M M M M M M M M M M M M M E Mouse M S M S M E M M M M M M M M M E M E Rat M M M M M M M S S E Canine M M M Feline M Porcine Primate M M M Equine Species Multi- SOLUBLE ANALYTE APPENDIX For Research Use Only. Multi- Species Equine M M Primate Porcine | 41 MerckMillipore.com/immunoassays Feline M M Canine M M M M assay R = RIA S = SMC™ assay map assay Rat M E M G ® M M M M M M M M Mouse M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M E Human Analyte Apo AII Apo B Apo CII Apo CIII Apo E APRIL/TNFSF13 ARG1 sAXL BAFF/Blys BCA-1/CXCL13 BDNF Betacellulin β-Endorphin β-2-Microglobulin BMP-9 BNP BRAK/CXCL14 C1q C2 C3 C3b/iC3b C4 C4b C5 C5a C9 CA125 CA15-3 CA19-9 Cadherin 13 (CDH13) Calbindin 9 (CA9) Carbonic Anhydrase Cathepsin D Caveolin-1 CCL28 sCD14 sCD30 SCD137/4-1BB/TNFRSF9 sCD163 sCD31/sPECAM-1 sCD40L CD44 CEA E = ELISA G = GyroMark™ HT assay M = MILLIPLEX M = HT assay E = ELISA G = GyroMark™ For Research Use Only. SOLUBLE ANALYTE APPENDIX 42 |MerckMillipore.com/immunoassays FABP3 FABP1 1 (ECM1) MatrixProtein Extracellular FABP4 Exodus-2/CCL21/6CKine Estradiol sE-Selectin Erythropoietin (EPO) Erythropoietin EpCAM Eotaxin-3/CCL26 Eotaxin-2/CCL24/MPIF-2 Eotaxin/CCL11 Endothelin-1 Endostatin Endoglin Endocan-1 (ESM-1) ENA-78/CXCL5 EGF DPP4 dPAPP-A DKK1 D-dimer Cystatin C CYFRA21-1 CXCL16 CTACK/CCL27 C-Peptide CRP (CKM) Creatine KinaseMuscle C-Peptide 2 Cortisol Corticosterone Contactin-1 Factor (CTGF) Connective TissueGrowth Complement Factor H(CFH) Complement Factor C3 CNTF Collagen IV Clusterin CK-MB sc-Kit/sSCFR CGA) Chromogranin-A (CHGA/ Analyte Human M ER M E M E M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M Mouse M E M M M M M M M M M M M M M M M M G M G M E Rat M M M M M M M M M M M E Canine E R M M M M Feline M M Porcine M M Primate M M M M M Equine M M M Species Multi- M M SOLUBLE ANALYTE APPENDIX For Research Use Only. E E R G E R G E R G Multi- Species M M M M M Equine M M M M M M M M M M M M M Primate M Porcine | 43 MerckMillipore.com/immunoassays M M M M M M M M Feline S M M M M M M M S Canine E E S M M M M M M M M M M assay R = RIA S = SMC™ assay map assay Rat M E M E M E M E M E M S ® E E S M M M M M M M M M M M M M M E M E M E M E M E M S Mouse M M M M M M M M M M M M M M M M M M M M M M M M M M E R M E M E M E M E M E M E M S M S M S M S M E R Human Analyte Factor B Factor H Factor I Factor sFAS/TNFRSF6 sFasL Ferritin A Fetuin FGF-1/FGF-acidic FGF-2/FGF-basic FGF-19 FGF-21 FGF-23 Follistatin (FST) Follistatin Flt3 Ligand Fibrinogen ProteinFibroblast Activation (FAP) Fibronectin Follistatin-like Protein 1 Follistatin-like (FSTL1) Fractalkine/CX3CL1 FSH Galectin 3 G-CSF GDF-15 GDNF GH Ghrelin (active) Ghrelin Ghrelin (total) Ghrelin GIP (total) Glial Fibrillary Acidic Protein (GFAP) GLP-1 (active) GLP-1 GLP-1 High Sensitivity GLP-1 (active) (total) GLP-1 GLP-2 Glucagon GM-CSF GOT1 sGP130 Granzyme B Granzyme A GSTα GROα/KC/CINC-1/CXCL1 GRO E = ELISA G = GyroMark™ HT assay M = MILLIPLEX M = HT assay E = ELISA G = GyroMark™ For Research Use Only. SOLUBLE ANALYTE APPENDIX 44 |MerckMillipore.com/immunoassays IL-1Ra IL-1β IL-1α IgM IgG4 IgG3 IgG2b IgG2a IgG2 IgG1 IGFBP7 IGFBP6 IGFBP5 IGFBP4 IGFBP3 IGFBP2 IGFBP1 IGF-2 IGF1R IGF-1 IgE IgA IFNγ IFNβ1 IFNβ IFNα2 sICAM-1 I-309/CCL1 (HSA) Human SerumAlbumin HSP70 HSP60 HMGB1 sHGFR/sc-Met HGF sHER3/sEGFR3/sErbB3 sHER2/sEGFR2/sErbB2 sHER1/sEGFR/sErbB1 Hepsin HE4 HCGβ HCC-4/CCL16 HCC-1/CCL14 HB-EGF Haptoglobin Analyte Human M S M S M S M E M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M Mouse M M M M M M M M M M M M M M Rat M M M M M Canine M Feline M M Porcine M M M M Primate M M M M Equine M M M Species Multi- SOLUBLE ANALYTE APPENDIX For Research Use Only. Multi- Species M M M M M M M M M M Equine M M M M M M M M M M M M M M M M M M M Primate M M M M M M M Porcine | 45 MerckMillipore.com/immunoassays M M M M M M M Feline M M M M M M M Canine M M M M M M M M M M assay R = RIA S = SMC™ assay map assay Rat ® M M M M M M M M M M M M M M M M M M M M M M M S M S M S M S M S M S M S M S M S M G Mouse S S M M M M M M M M M M M M M M M M M M M M M M M M M E M S M S M S M S M S M S M S M S M S M S M S M S M S M G M S G Human Analyte sIL-1RI sIL-1RII IL-2 sIL-2Rα IL-3 IL-4 sIL-4R sIL-4R IL-5 IL-6 sIL-6R sIL-6Rα IL-7 IL-8/CXCL8 IL-9 IL-10 IL-12 (p40) IL-12 IL-11 IL-12/23 (p40) IL-12/23 (p70) IL-12 IL-12 IL-13 IL-14/α-Taxilin IL-15 IL-16 IL-17A/CTLA8 Il-17E/IL-25 IL-17A/F IL-17F IL-18 IL-19 IL-20 IL-21 IL-22 IL-23 IL-24 IL-27 IL-28B/IFNλ3 IL-28A/IFNλ2 IL-29/IFNλ1 IL-31 IL-34 IL-32α (mature) IL-33/NF-HEV IL-35 E = ELISA G = GyroMark™ HT assay M = MILLIPLEX M = HT assay E = ELISA G = GyroMark™ For Research Use Only. SOLUBLE ANALYTE APPENDIX 46 |MerckMillipore.com/immunoassays Melatonin Protein(MIA) Activity Melanoma Inhibitory MDHI Mesothelin MDC/CCL22 M-CSF MCP-5/CCL12 MCP-4/CCL13 MCP-3/CCL7 MCP-2/CCL8 MCP-1/CCL2 MCAM/MUC18/sCD146 (MBL) Mannose-binding lectin Lymphotactin/XCL1 sL-Selectin glycoprotein 1(LRG1) Leucine-rich alpha-2- LIX/CXCL6/GCP-2 LIGHT LIF LH OB-R Leptin Receptor (LEPR)/ Leptin LDLR Laminin Lactotransferrin (LTF) KIM-1 Keratin-6 Keratin-1, 10 KC-like Protein Kallekrein-6 I-TAC/CXCL11 Irisin IP-10/CXCL10 Involucrin Insulin Specific Insulin Lispro Insulin (AnimalSerumFree) Insulin IL-38/IL-1F10 IL-37/IL-1F7 IL-36β/IL-1F8 Analyte Human M EG M ER M S M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M R E E Mouse M EG M E M M M M M M M M M M M M ERG M G M E Rat M M M M M M M Canine M E M M M M M Feline M M M Porcine R Primate M M M M M Equine M M Species Multi- R R SOLUBLE ANALYTE APPENDIX For Research Use Only. Multi- Species Equine M M M Primate Porcine | 47 MerckMillipore.com/immunoassays Feline M Canine E E M M M M M M M assay R = RIA S = SMC™ assay map assay Rat M G ® E E M M M M M M M M M M M M M M M M M Mouse E M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M Human Analyte Midkine MIF MIG/CXCL9 MIP-1α/CCL3 MIP-1β/CCL4 MIP-1δ/MIP-5/CCL15 MIP-2/CXCL2 MIP-3α/CCL20 MIP-3β/CCL19 MIP-4/PARC/CCL18 MMP-1 MMP-2 MMP-3 MMP-7 MMP-8 MMP-9 MMP-10 MMP-12 MMP-13 MPIF/CCL23 MR-ProADM (MPO) Myeloperoxidase Myoglobin Myostatin/GDF8 Myostatin Light Chain 3Myostatin (MYL3) NAP-2/CXCL7 sNCAM NCAML1/L1CAM/CD171 Neuropeptide Y (NPY) sNeuropilin-1 (sNRP-1) Neurotensin Neuron-specific Enolase (NSE) Neutrophil Elastase-2 (ELA2) NGAL/Lipocalin-2 NGF Notch1 NT proBNP Oncostatin-M (OSM) Orexin A Orexin Osteoactivin Osteocalcin (OC) Osteocrin (OSTN)/Musclin E = ELISA G = GyroMark™ HT assay M = MILLIPLEX M = HT assay E = ELISA G = GyroMark™ For Research Use Only. SOLUBLE ANALYTE APPENDIX 48 |MerckMillipore.com/immunoassays S100B Resistin Renin RBP4 RANTES/CCL5 RANKL sRAGE PTH PTGDS PYY (total) PYY PYY (3-36)Specific sP-Selectin PSA (total) PSA (free) Properdin/Factor D ProMMP-9 Prolactin Proinsulin Progranulin (pGRN) Progesterone PIIANP CXCL4 Platelet Factor 4(PF4)/ (PLGF-2) Factor Growth Placental 2 (PLGF) Factor Growth Placental PLA2G7 Neurofilament (pNF-H) Phosphorylated Perforin Pentraxin-3 (PTX3) PEDF PDGF-BB PDGF-AB/BB PDGF-AA PARK7/DJ1 PARK5/UCHL1 Periostin Pancreatic Polypeptide (PP) PAI-1 (total) Oxytocin Osteoprotegerin (OPG) Osteopontin (OPN) Osteonectin/SPARC Analyte Human M EG M ER M E M E M E E R M M M M M M M M M M M M M M M M M M M M M M M M M M M M M R E Mouse M M M M M M M M M M M M M M M M R Rat M M M M M M M M M M M R Canine M M M M M Feline M M M M M Porcine M Primate M M M M M Equine M M Species Multi- M E SOLUBLE ANALYTE APPENDIX For Research Use Only. M M M M M M Multi- Species M M M M M M M Equine M M M M M M M M M Primate M M M M M M M Porcine | 49 MerckMillipore.com/immunoassays M M M M M M Feline M M M M M M M M Canine M M M M M M M M M M M assay R = RIA S = SMC™ assay map assay Rat ® E M M M M M M M M M M M M M M M M M S Mouse M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M M S Human Analyte Sclerostin (SOST) SAA-3 SCF SDF-1/CXCL12 SDH A Serum Amyloid P (SAP) Serum Amyloid Sex Hormone Binding Globulin (SHBG) sST2/IL1RL1 Substance P Soluble Superoxide Soluble Superoxide Dismutase (sSOD1) 1 Soluble Superoxide Dismutase 2 (sSOD2) Syndecan 4 (SYND4) T3 T4 TARC/CCL17 pTau (Thr181) pTau (total) Tau (TN-C) Tenascin-C Testosterone TFF-3 TGFα TGFβ1 TGFβ2 TGFβ3 Thrombomodulin Thrombospondin-1 (TSP-1) Thrombospondin-2 (TSP-2) sTIE-2 TIMP-1 TIMP-2 TIMP-3 TIMP-4 (TF) Tissue Factor TNFα TNFβ/Lymphotoxin-α (LTA) TNFβ/Lymphotoxin-α sTNF RI sTNF RII TPO TRAIL/TNFSF10 2 (TGM2) Transglutaminase TRAP5 E = ELISA G = GyroMark™ HT assay M = MILLIPLEX M = HT assay E = ELISA G = GyroMark™ For Research Use Only. SOLUBLE ANALYTE APPENDIX 50 |MerckMillipore.com/immunoassays E =ELISAGGyroMark™ HTassay M=MILLIPLEX von WillebrandFactor (vWF) YKL40/CHI3L1 DBindingProtein Vitamin sVEGFR3/sFlt-4 sVEGFR2/sKDR/sFlk-1 sVEGFR1/sFlt-1 VEGF-D VEGF-C VEGF-A VEGF sVCAM-1 ucMGP Uromodulin suPAR TWEAK TSLP TSH TroponinCardiac T(cTnt) Cardiac TroponinCardiac I(cTnI) Troponin T(TnT) Troponin I(TnI) Analyte Human M E M M M M M M M M M M M M M M M M M S S Mouse M M M M M M M M M ® M S Rat mapassay R=RIASSMC™assay M M M M Canine M S Feline Porcine Primate M M S Equine Species Multi- CELL SIGNALING ANALYTE APPENDIX For Research Use Only. M2 M2 M2 M2 MK MK MK MK MK MK MK MK MM Rat M2 S M2 S

MK M2 MK M2 MK M2 MM MK M2 MM MK M2 MM MK M2 MM

M2 M2 M2 M2 MK MK MK MK MK MK MK MK MK MM MM MM M2 S M2 S MAPmate™ assay MAPmate™ MK M2 MK M2 Mouse MK M2 MM MK M2 MM MK M2 MM MK M2 MM MAPmate™ assay S = SMC™ assay assay map MAPmate™ map ®

® | 51 MerckMillipore.com/immunoassays MILLIPLEX M2 M2 M2 M2 MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MM MM M2 S M2 S MK M2 MK M2 MK MM Human SMC™ (Single Molecule Counting) assay SMC™ (Single Molecule MK M2 MM MK M2 MM MK M2 MM MK M2 MM S = MM =

Phospho/Total 2 Plex Kit MM = MILLIPLEX map Phospho/Total ®

Phospho/Total 2 Plex Kit Phospho/Total map Kit map

® ® map Kit M2 = MILLIPLEX ®

= MILLIPLEX = MILLIPLEX

Erk/MAPK 1/2 (total) ErbB1/EGFR (pan Tyr) ErbB2/HER2 (pan Tyr) ErbB3/HER3 (pan Tyr) ErbB4/HER4 (pan Tyr) Erk/MAPK 1/2 (Thr185/Tyr187) eIF-4G (Ser1108) eIF-4G eIF-4E (Ser209) eIF-4E eIF-4B (Ser422) eIF-4B eIF2a (Ser51) CREB (total) c-Kit (pan Tyr) c-Met/HGFR (pan Tyr) (total) c-Myc CREB (Ser133) Chk2 (Thr68) (Ser73) c-Jun Blk (Tyr389) Caspase 3 (Active) (total)Caspase 8, Active (total)Caspase 9, Active CD3Ɛ (pan Tyr) Chk1 (Ser345) ATR (total) ATR (Ser112) BAD Bcl-2 (Ser70) (total) β-Tubulin ATF2 (Thr71) ATF2 Akt3 (total) Akt3 (Ser472) Akt2 (total) Akt2 (Ser474) Akt1 (total) Akt1 (Ser473) Akt/PKB (total) Akt/PKB (Ser473) 4E-BP1 (total) Analyte 4E-BP1 (Thr37/46) MK = MILLIPLEX the type of kit: MK

How to use the appendix How to letter The alphabetic in the chart represents analytesCell Signaling order. appear in alphabetic Cell Analyte Signaling Appendix M2 For Research Use Only. CELL SIGNALING ANALYTE APPENDIX 52 |MerckMillipore.com/immunoassays Fgr (Tyr412) FGFR1 (panTyr) FADD (Ser194) Analyte Fyn (Tyr420) Flt3 (panTyr) GSK3α (Ser21) GSK3α GAPDH (total) GSK3α (total) GSK3α GSK3β (Ser9) GSK3β GSK3β (total) GSK3β H2A.X (Ser139) Hck (Tyr411) IGF1R (Tyr1135/1136) IGF1R (panTyr) HSP27 (Ser78) IGF1R (total) IR (total) IR (total) IR (Tyr1162/1163) IR (panTyr) IRS1 (Ser636) IRS1 (total) JNK/SAPK1 (Thr183/Tyr185) Iκκα/β (Ser177/181) IκBα (Ser32) JNK/SAPK1 (total) Lck (panTyr) LAT (panTyr) Lck (Tyr394) Lyn (Tyr397) MDM2 (total) MEK1 (Ser222) MSK1 (Ser212) MSCFR (panTyr) mTOR (Ser2448) NFκB (Ser536) mTOR (total) NFκB (total) p38/SAPK2A/B (Thr180/Tyr182) p21 (total) p38/SAPK2A/B (total) p70S6 Kinase(Th389/412) p53 (Ser46) p53 (Ser15) p70S6 Kinase(total) PDGFRα (panTyr) PARP, Cleaved (total) PTEN (Ser380) PDGFRβ (panTyr) MK M2MM MK M2MM MK M2MM MK M2MM MK M2MM MK M2MM Human MK M2 MK M2 MM MM MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK M2MM MK M2MM MK M2MM MK M2MM MK M2MM MK M2MM Mouse MK M2 MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK M2 MK M2MM MK M2MM MK MM M2 MM MK M2 MK M2 MK M2 Rat MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK APPENDIX

CELL SIGNALING ANALYTE APPENDIX For Research Use Only. MK MK MK MK MK MK MK MK MK MK MK MK MK MK Rat MK M2 MK M2 MK MK MK MK MK MK MK MK MK MK MK MK MK MK MM MAPmate™ assay S = SMC™ assay assay MAPmate™ MK M2 MK M2 Mouse MK MM map

® | 53 MerckMillipore.com/immunoassays MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MK MM MK M2 MK M2 MK MM Human Phospho/Total 2 Plex Kit MM = MILLIPLEX Phospho/Total map

® map Kit M2 = MILLIPLEX

® ZAP-70 (pan Tyr) ZAP-70 Yes (Tyr421) Yes VEGFR1/Flt-1 (pan Tyr) VEGFR1/Flt-1 (pan Tyr) VEGFR2/KDR/Flk-1 (pan Tyr) VEGFR3/Flt-4 TSC2 (total) STAT6 (Tyr641) STAT6 (pan Tyr) Syk (total) TGFβRII TIE1 (pan Tyr) TIE2 (pan Tyr) TNFR1 (total) TSC2 (Ser939) STAT5A/B (total) STAT5A/B STAT5A/B (Tyr694/699) STAT5A/B STAT3 (total) STAT3 STAT3 (Ser727) STAT3 STAT1 (total) STAT1 (Tyr690) STAT2 (Tyr705) STAT3 STAT1 (Tyr701) STAT1 Src (Tyr419) SMAD4 (total) SMAD3 (Ser423/425) SMAD2 (Ser465/467) RPS6 (total) RPS6 (Ser235/236) PTEN (total) PTEN Analyte MK = MILLIPLEX Lit. Code SLN_MRK 2017-06097 10/2017