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Myopalladin Gene Polymorphism Is Associated with Rabbit Meat Quality Traits

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Myopalladin Gene Polymorphism Is Associated with Rabbit Meat Quality Traits Italian Journal of Animal Science ISSN: (Print) 1828-051X (Online) Journal homepage: http://www.tandfonline.com/loi/tjas20 Myopalladin gene polymorphism is associated with rabbit meat quality traits Jie Wang, Yu Shi, Mauricio A. Elzo, Yuan Su, Xianbo Jia, Shiyi Chen & Songjia Lai To cite this article: Jie Wang, Yu Shi, Mauricio A. Elzo, Yuan Su, Xianbo Jia, Shiyi Chen & Songjia Lai (2017) Myopalladin gene polymorphism is associated with rabbit meat quality traits, Italian Journal of Animal Science, 16:3, 400-404, DOI: 10.1080/1828051X.2017.1296333 To link to this article: http://dx.doi.org/10.1080/1828051X.2017.1296333 © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. Published online: 02 Mar 2017. Submit your article to this journal Article views: 113 View related articles View Crossmark data Full Terms & Conditions of access and use can be found at http://www.tandfonline.com/action/journalInformation?journalCode=tjas20 Download by: [University of Florida] Date: 29 July 2017, At: 12:51 ITALIAN JOURNAL OF ANIMAL SCIENCE, 2017 VOL. 16, NO. 3, 400–404 http://dx.doi.org/10.1080/1828051X.2017.1296333 SHORT COMMUNICATION Myopalladin gene polymorphism is associated with rabbit meat quality traits aà aà b a a a a Jie Wang , Yu Shi , Mauricio A. Elzo , Yuan Su , Xianbo Jia , Shiyi Chen and Songjia Lai aCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China; bDepartment of Animal Science, University of Florida, Gainesville, FL, USA ABSTRACT ARTICLE HISTORY The objective of this study was to investigate the effect of the polymorphism in the Myopalladin Received 23 May 2016 (MYPN) gene on meat quality traits in the Hyla, Champagne, Tianfu Black rabbit breeds using Revised 24 August 2016 PCR and DNA sequencing. A novel SNP (g.18497416 G > A) was found at 229 bp in exon 13 of Accepted 3 December 2016 chromosome 18. The three rabbit populations had intermediate levels of genetic diversity KEYWORDS according to their polymorphism information content values. The statistical analysis indicated à à Rabbit; MYPN that rabbits with the GG genotype had a significantly greater a 24h and b 24h than rabbits with polymorphism; PH; meat the AA genotype (p < .05) in the longissimus dorsi muscle and that animals with the AA geno- à à colour; intramuscular fat type had substantially higher a 0h and a 24h than those with the AG genotype (p < .05) in the biceps femoris muscle. The rabbits with the AA genotype had the highest intramuscular fat (IMF) values and those with the GG genotype had the lowest IMF values in both longissimus dorsi and biceps femoris muscles. These results indicated that MYPN could be considered as candidate genes for genetic improvement of rabbit meat traits. Introduction studied until recently. The gene is located in Chromosome 18 and has118,864 bp length including The myopalladin (MYPN) gene codes for a multifunc- 22 exons, 21 introns and several unknown sequence tional protein that plays a central role in the organisa- gaps (http://asia.ensembl.org/). A large number of tion and tethering of nebulin and a-actinin in researches have conducted studies on rabbit meat vertebrate Z-lines (McElhinny et al. 2003). This is a sig- quality traits at a molecular level and identified single nature domain of this protein family and may have a nucleotide polymorphisms in the MSTN, MC4R, special role in creating the highly ordered cytoskeleton POU1F1, CAST, Myf5 and MYf6 genes that were associ- of the sarcomere (Gilbert et al. 1999). Recently, swine ated with meat traits (Jiang et al. 2008; Yin et al. 2011; research showed that single nucleotide polymorphisms Fontanesi et al. 2013; Qiao et al. 2014; Sternstein et al. in the MYPN gene were associated with lean cuts (LC), 2014; Wang et al. 2015; Yin et al. 2015; Wang et al. visible intermuscular fat (VIF), meat colour and tender- 2016, 2017). However, none of these studies consid- ness (Wimmers et al. 2007; Zhai et al. 2010; Braglia ered associations between MYPN SNPs and rabbit et al. 2013). Research in beef cattle indicated that SNP meat quality traits. Thus, the objective of this research of the MYPN gene affected loin-eye area and water- was to investigate a single nucleotide polymorphism holding capacity of beef (Jiao et al. 2010) and loin in the MYPN gene and to explore the association of muscle area (Hong et al. 2011). this variant with meat quality traits in the Hyla, Rabbit meat has lower fat content and high protein Champagne, Tianfu Black rabbit breeds. level than meat from other animals, thus it could pro- vide consumers with abundant bioactive compounds (Dalle Zotte 2002; Dalle Zotte & Szendro 2011). Materials and methods Further, rabbit meat has been considered to be a func- Animals tional food source that can effectively reduce public health costs (Dalle Zotte & Szendro 2011). However, We investigated 373 unrelated individuals, each from the rabbit MYPN gene has not been thoroughly a separate litter (i.e. number of litters ¼ 373), CONTACT Dr. Songjia Lai [email protected] College of Animal Science and Technology, Sichuan Agricultural University, Huimin road 211#, 611130 Chengdu, China à Jie Wang and Yu Shi contributed equally to this work. ß 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ITALIAN JOURNAL OF ANIMAL SCIENCE 401 representing three rabbit breeds from the rabbitry of likelihood ratio test with POPGENE software (Ver. 3.2). the Institute of Animal Genetics and Breeding, Sichuan Population genetic indexes (He, Ne and PIC) were Agricultural University in China: Hyla (137), obtained using methodology described by Nei and Champagne (147) and Tianfu Black (89). Rabbits were Roychoudhury (1974) and Botstein et al. (1980). all slaughtered together (i.e. on the same day) at 70 The linear model used to analyse the meat quality days of age and samples of ear tissue were collected traits was as follows: for DNA extraction. Carcasses were kept at 4 C for Yijkl ¼ l þ Bi þ Gj þ Sk þ eijkl 24 hours. Tissue samples were taken from the longissi- l mus dorsi and biceps femoris muscles. Measurements where Yijkl was a meat quality trait, was the overall à à à mean for each trait, B was the breed effect, G was the of pH (pH0h) and colour (L 0h,a 0h,b 0h) were taken i j within 15 min after slaughter. Next, after carcases had genotype effect, Sk was the fixed sex effect and eijkl been chilled for 24 at 4 C, measurements were taken was the residual effect. Litter effect was not included à à à in the model because each rabbit came from a separ- for pH (pH24 h), colour (L 24 h,a24 h,b24 h) and intra- muscular fat (IMF). The traits of the longissimus dorsi ate litter. A random polygenic animal effect was not and biceps femoris muscles including the pH, colour included in the model because rabbits were unrelated, and IMF were measured following the methods thus random animal genetic effects were confounded described in Van Laack et al. (2000) and AOAC (1980) with random residual effects. Initial versions of the on the same two days (i.e. on the day of slaughter statistical analyses included interactions between and 24 hours postmortem). All experimental proce- breed and genotype and between sex and genotype. dures were approved by the Institutional Animal Care None of these interactions were significant, thus they and Use Committee of Sichuan Agricultural University. were excluded from the final model. Least squares means and their standard errors were computed for all genotype effects, and pairwise comparisons among Detection of SNP and genotyping them were made using the Bonferroni t-tests. Genomic DNA was extracted using AxyPrep Genomic Computations were carried out using the general DNA Miniprep Kit (Axygen, Union City, CA) and stored linear model (GLM) procedure of SPSS 21 (IBM, À at 80 C. The PCR primers were designed by the Armonk, NY). Primer Premier 6 software based on the rabbit gene sequence (Ensembl accession NO. Results and discussion ENSOCUG00000009939). The PCR primers for the MYPN gene were as follows: MYPN-F: GGTGGTAAC We detected one single nucleotide polymorphism ACATTTCCCTCCTC, MYPN-R: AAGGAATCCTCTGATGGA (g.18497416 G > A, in chromosome 18) located at GAATCC. These PCR primers were used to amplify a 229 bp in exon 13. The SNP resulted in amino acid 591 bp fragment of exon 13 in the MYPN gene. The changes from serine to glycine. The genotype and 25 lL reaction mixture contained 50 ng genomic DNA, allele frequencies, Chi-square test, heterozygosity (He), 1 lM of each primers, 1.5 mM MgCl2, 200 lM dNTPs effective number of allele (Ne), and polymorphism (dCTP, dGTP, dATP and dTTP), and 0.3 units of Taq information content (PIC) were calculated and sum- DNA polymerase (MBI). The PCR protocol involved an marised in Table 1. Chi-square tests showed that geno- initial denaturation at 95 C for 5 min, 38 cycles of typic frequencies agree with the Hardy–Weinberg denaturing at 95 C for 40 s, annealing at 55.0 C for equilibrium in the three rabbit populations (p > .05). 45 s, extension at 72 C for 40 s, with a final extension The three rabbit populations had intermediate levels at 72 C for 10 min. The PCR products were directly of genetic diversity according to their PIC values.
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