DOCSLIB.ORG

Signature Redacted Angela M

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Signature Redacted Angela M Environmental remediation and biofuel production through nanoparticle stimulation of MASSACHUSETTS INSTITUTE yeast OF TECHNOLOGY 03 2019 by JUL Shalmalee Pandit LIBRARIES ARCHIVES B.S. Bioengineering The University of California, Berkeley (2015) Submitted to the Department of Biological Engineering in Partial Fulfillment of the Requirements for the Degree of Master of Science in Biological Engineering at the Massachusetts Institute of Technology June 2019 ©2019 Massachusetts Institute of Technology All rights reserved. redacted Signature of A uthor.........................&1............Signature .....--. ......... Department of Biological Engineering May 23, 2019 Certified.....Signature redacted Angela M. Belcher Professor, Department of Biological Engineering Thesis Supervisor Signature redacted Accepted by ........ ........................ Forest White Chair, Department Committee on Graduate Students 77 Massachusetts Avenue Cambridge, MA 02139 MITLibraries http'l'ibraries.mitedu/ask DISCLAIMER NOTICE Due to the condition of the original material, there are unavoidable flaws in this reproduction. We have made every effort possible to provide you with the best copy available. Thank you. The images contained In this document are of the best quality available. Environmental remediation and biofuel production through nanoparticle stimulation of yeast by Shalmalee Pandit Submitted to the Department of Biological Engineering On May 23, 2019 in Partial Fulfillment of the Requirements for the Degree of Master of Science in Biological Engineering Abstract Artificially photosynthetic systems aim to store solar energy and chemically reduce carbon dioxide. These systems have been developed in order to use light to drive processes for carbon fixation into biomass and/or liquid fuels. We have developed a hybrid-biological system that manages both genetically controlled generation of products along with the photoactivability of a semiconductor system. We show an increase in the production of ethanol, a common biofuel, through the electron transfer stimulated by biologically produced cadmium sulfide nanoparticles and light. This work provides a basis on which to improve the production of many metabolites and products through endogenously produced nanoparticles. Thesis Supervisor: Angela M. Belcher Title: Professor, Department of Biological Engineering 2 Contents 1 Chapter 1 Introduction ..................................................... .... ........... .... 7 1.1 Environmental and health effects due to cadmium ......................................... 7 1.1.1 Environmental cleanup of cadmiumc.a.............................. .................... 7 1.1.2 Current methods of cadmium ion removal............................................. 7 1.2 Current state of biofuel production ........................... .......................... 9 1.2.1 Drivers of biofuel production ................. ............................................. 9 1.2.2 Biofuel production to date... --..... ........... ....... 9 1.2.3 Key issues and considerations for biofuel production ......................... 10 2 Chapter 2 Characterization of yeast-hybrid system .................................... 11 2.1 Design of yeast-inorganic hybrid system .................. 11 2.2 Visual characterization of system........................................... 12 2.2.1 Transmission electron microscopy (TEM) images of system .............. 13 2.2.2 Elemental mapping analysis .--..... - .......-- ... ................. 14 2.3 Experimental design to use hybrid system ................. 17 2.4 Transcriptomic characterization of system................. 18 2.4.1 Principal component analysis of RNA sequencing data...................... 18 2.4.2 Gene expression fold change induced by cadmium treatment ..... 19 2.4.3 Gene expression fold change induced by light treatment....... 20 2.5 Experimental Methods........... ................. 21 2.5.1 Yeast strain and culture ... ................. ....... ........................ 21 2.5.2 Light experiments............................ 22 2.5.3 Transmission electron microscopy (TEM)................ 22 2.5.4 RNA sequencing and analysis .. .................... ................... 23 3 Chapter 3 Further characterization and effects of transcriptomic changes on metabolite production ......-............--...... ............... 24 3.1 Further characterization through gene set enrichment analysis (GSEA)... 24 3.2 Intracellular ATP levels in yeast strains ................................... 26 3.3 Intracellular glucose levels in yeast strains ........................ -................... 27 3 3.4 Intracellular NAD+ and NADH Concentration.......................................... 28 3.5 Discussion, conclusions, and future work ....................................................... 30 3.6 Experim ental M ethods.................. .............................................................. 31 3.6.1 Gene set enrichment analysis (GSEA) ...................... 31 3.6.2 Yeast lysate preparation ........................................................................ 31 3.6.3 Measuring intracellular ATP concentration .............. 31 3.6.4 Measuring intracellular glucose concentration .................................. 31 3.6.5 Measuring intracellular NAD+ and NADH ......................................... 31 4 Chapter 4 Producing biofuel using hybrid-biological system.......................... 32 4.1 Potential mechanistic explanation to produce biofuel (ethanol)............... 32 4.2 Measuring intracellular ethanol concentration......................................... 32 4.3 Implementing and characterizing the mutation in another yeast strain .... 33 4.3.1 Visual characterization of Y567 AMet17 ............................................. 33 4.3.2 Elemental m apping analysis .................................................................. 35 4.3.3 Measuring intracellular ATP concentration .................................... 38 4.3.4 Measuring the NAD+/NADH ratio ....................... 39 4.4 Intracellular ethanol concentration in Y567 AMet17 strain ............ 40 4.5 Discussion, conclusions, and future work ........ ........................................... 41 4.6 Experim ental M ethods............................................................................... 42 4.6.1 Yeast strain and culture methods......................... 42 4.6.2 Knocking out M et17 in Y567 ................................................................. 42 4.6.3 Measuring intracellular ethanol concentration................................... 42 B ibliography .................................................................................................................. 43 Acknowledgements ......................................... 48 4 List of Figures Figure 2-1 Yeast-hybrid biological system. a. Schematic of yeast-hybrid system. Knocking out a pathway in thiol production lead to an increase in hydrogen sulfide production. This hydrogen sulfide is shuttled out of the cell. b. When the yeast cells are treated with cadmium ions, cadmium sulfide nanoparticles are precipitated on the yeast's cell surface. The CdS nanoparticles excite at UV wavelengths........... 12 Figure 2-2 Wild-type yeast treated with cadmium ions display no CdS nanoparticles in the cell surface. ........................................................................................................... 13 Figure 2-3 Mutated yeast (AMet17) when treated with cadmium, precipitate CdS nanoparticles on the cell surface. ......................................................................... 14 Figure 2-4 CdS nanoparticles displayed on the yeast cell surface............... 15 Figure 2-5 Elemental mapping analysis of cadmium on TEM image.................... 15 Figure 2-6 Elemental mapping analysis of sulfur on TEM image. ........................ 16 Figure 2-7 Elemental analysis displaying both cadmium (red) and sulfur (blue) superim posed on the TEM im age. ..................... ................................................... 17 Figure 2-8 Experimental conditions for light and dark experiments. ................... 18 Figure 2-9 PCA plot displaying RNA Sequencing data........................................... 19 Figure 2-10 Log fold change volcano plot depicting the effects of the cadmium sulfide nanoparticles on the transcriptome of the yeast-hybrid system................ 20 Figure 2-11 Log fold change volcano plot depicting the effects of the light treatment on the transcriptome of the yeast-hybrid system. .................................................. 21 Figure 3-1 Gene set enrichment analysis of protein coding genes involved in ATP production and synthesis due to the presence of light. The p-value is less than 0.05. Random walk is displayed as well........................................................................... 25 Figure 3-2 Gene set enrichment analysis of genes involved in glycolysis due to the presence of cadmium sulfide nanoparticles on the yeast's cell surface. The p-value is less than 0.05. Random walk is also displayed. ....................................................... 26 Figure 3-3 ATP concentration in various yeast strains. ATP concentration was calculated per cell. Yellow plots indicate light treatment....................................... 27 Figure 3-4 Intracellular glucose concentration displayed in various yeast strains. Yellow bars indicate light treatm ent. ...................................................................... 28 Figure 3-5 Total NAD+ and NADH in
Load more

Recommended publications
  • Advancement of Biodiesel in Bangladesh
    IOSR Journal of Engineering (IOSRJEN) www.iosrjen.org ISSN (e): 2250-3021, ISSN (p): 2278-8719 Vol. 06, Issue 06 (June. 2016), ||V2|| PP 59-64 Advancement of Biodiesel in Bangladesh A. Z. A. Saifullah1, Md. Abdul Karim2, Md. Raisul Karim3 1, 2Department of Mechanical Engineering, IUBAT – International University of Business Agriculture and Technology, Dhaka 1230, Bangladesh 3Department of Mechanical Engineering, Dhaka University of Engineering and Technology, Gazipur 1700, Bangladesh Abstract:–This paper presents an overview of Biodiesel resources and research in Bangladesh. The energy sector of Bangladesh largely depends on natural gas and petroleum oil. But the reserves are inadequate to meet the energy demand for long term economic growth. Biodiesel can be an alternative to these fossil fuels. Biodiesel is non-toxic and biodegradable. The combustion of Biodiesel emits very less amount of COX, SOX, hydrocarbons and particulate matter. In Bangladesh, a good number of edible and non-edible Biodiesel feedstock is available. But the edible sources are not promising as they need arable lands for cultivation. The arable lands in Bangladesh are used for food production. About 47750 km of road and railway side arid lands can be used to produce non-edible Biodiesel feedstock. But Algae can be the most effective source of Biodiesel. Algae possess high productivity and high lipid content. Producing 1kg of Algae Biodiesel can fix 1.83 kg of CO2. Algae can be produced in non-arable lands, fresh water, salt water and waste water. In Bangladesh, about 4.418 million hectares of infertile land, 1.383 million hectares of water areas (lakes, rivers, costal saline water etc.) and 0.31 million hectares of ponds are available which can be used for Algae production.
    [Show full text]
  • Uses of Non Edible Jatropha Seeds – Review M
    International Journal of Multidisciplinary Research and Modern Education (IJMRME) ISSN (Online): 2454 - 6119 (www.rdmodernresearch.com) Volume II, Issue II, 2016 USES OF NON EDIBLE JATROPHA SEEDS – REVIEW M. Vivek*, Dr. P. K. Srividhya** & Dr. K. Sujtha*** * Research Scholar, Periyar Maniammai University, Vallam, Thanjavur, Tamilnadu ** Professor, Department of Mechanical Engineering, Periyar Maniammai University, Thanjavur, Tamilnadu *** Professor, Department of Electrical and Electronics Engineering, Dr.M.G.R University, Chennai, Tamilnadu Abstract: For the most part creating nation are confronted such a variety of issues, for example, Transport, Energy, Food, Cloth, Employment, Education and so forth., now a day's the greater part of the vehicle are worked with diesel or petrol. The accessibility of these fluid powers is decreased because of the gigantic utilization of transportation. Normally the accessibility of energizes shifted depending up on surface of the earth. Couple of nations are holding enormous limit of oils. The remaining is buy from those nations. The expense of the fuel is additionally fluctuated depending up on the accessibility; henceforth the world exchanges can't ready to foresee the expense of the oil and to minimize the fuel utilization. From this basic circumstance compelled to locate another fuel which is utilized to infer the source as much as long. It starts to deliver oils from non palatable seed cakes. Jatropha curcas plant is one of the oil plants. The Jatropha seed cakes are utilized to deliver oils. The oil was mixed with Diesel and infers the vehicle. It gives closest proficiency when contrasted and immaculate diesel. At that point the administration starts to plant Jatropha curcas trees and deliver oil from itself.
    [Show full text]
  • Physiological, Biochemical, and Molecular Mechanisms of Heat Stress Tolerance in Plants
    Int. J. Mol. Sci. 2013, 14, 9643-9684; doi:10.3390/ijms14059643 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 www.mdpi.com/journal/ijms Review Physiological, Biochemical, and Molecular Mechanisms of Heat Stress Tolerance in Plants Mirza Hasanuzzaman 1,*, Kamrun Nahar 2,3, Md. Mahabub Alam 2, Rajib Roychowdhury 4 and Masayuki Fujita 2,* 1 Department of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Dhaka 1207, Bangladesh 2 Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan; E-Mails: [email protected] (K.N.); [email protected] (M.M.A.) 3 Department of Agricultural Botany, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka 1207, Bangladesh 4 Department of Biotechnology, Visva-Bharati University, Santiniketan 731235, West Bengal, India; E-Mail: [email protected] * Authors to whom correspondence should be addressed; E-Mails: [email protected] (M.H.); [email protected] (M.F.); Tel.: +8187-891-3133 (M.F.); Fax: +8187-891-3021 (M.F.). Received: 1 February 2013; in revised form: 16 April 2013 / Accepted: 19 April 2013 / Published: 3 May 2013 Abstract: High temperature (HT) stress is a major environmental stress that limits plant growth, metabolism, and productivity worldwide. Plant growth and development involve numerous biochemical reactions that are sensitive to temperature. Plant responses to HT vary with the degree and duration of HT and the plant type. HT is now a major concern for crop production and approaches for sustaining high yields of crop plants under HT stress are important agricultural goals.
    [Show full text]
  • 40517-013: Public-Private Infrastructure Development Facility
    Completion Report Project Numbers: 40517-013 and 40517-042 Loan Numbers: 2453 and 2454 Grant Numbers: 0253 and 0254 TA Number: 7143 September 2018 Bangladesh: Public–Private Infrastructure Development Facility This document is being disclosed to the public in accordance with ADB’s Public Communications Policy 2011. CURRENCY EQUIVALENTS Currency Unit – taka (Tk) At Appraisal At Project Completion 24 June 2008 31 December 2014 Tk1.00 = $0.01459 $0.01283 $1.00 = Tk68.5200 Tk77.9200 ABBREVIATIONS ADB – Asian Development Bank ADF – Asian Development Fund DMF – design and monitoring framework ESSF – environmental and social safeguards framework FAPAD – Foreign Aided Project Audit Directorate IDB – Islamic Development Bank IDCOL – Infrastructure Development Company Limited JICA – Japan International Cooperation Agency LIBOR – London interbank offered rate MW – megawatt OCR – ordinary capital resources PPIDF Public–Private Infrastructure Development Facility PPP – Public–private partnership RRP – report and recommendation of the President SDR – special drawing rights SHS – solar home systems TA – technical assistance NOTES (i) The fiscal year (FY) of the Government of Bangladesh ends on 30 June. FY before a calendar year denotes the year in which the fiscal year ends, e.g., FY2018 ends on 30 June 2018. (ii) In this report, "$" refers to United States dollars. Vice-President Wencai Zhang, Operations 1 Director General Hun Kim, South Asia Department (SARD) Director Manmohan Parkash, Bangladesh Resident Mission, SARD Team leader Bidyut Kumar Saha, Senior Project Officer, SARD Team members Kamrun Nahar Chowdhury, Operations Assistant, SARD M.M. Zimran Khan, Associate Project Analyst, SARD Md. Golam Mortaza, Economist, SARD In preparing any country program or strategy, financing any project, or by making any designation of or reference to a particular territory or geographic area in this document, the Asian Development Bank does not intend to make any judgments as to the legal or other status of any territory or area.
    [Show full text]
  • Kamrun Nahar Doctor of Philosophy November 2015
    A Low Cost Method for Glyphosate Analysis, and Site Investigation and Modelling of Glyphosate Fate and Transport from Genetically Modified Canola Farmland in Parkes, NSW, Australia Kamrun Nahar A dissertation in fulfilment of the requirements for the degree of Doctor of Philosophy School of Engineering and Information Technology The University of New South Wales Canberra, Australia November 2015 ORIGINALITY STATEMENT 'I hereby declare that this submission is my own work and to the best of my knowledge it contains no materials previously published or written by another person , or substantial proportions of material which have been accepted for the award of any other degree or diploma at UNSW or any other educational institution, except where due acknowledgement is made in the thesis. Any contribution made to the research by others, with whom I have worked at UNSW or elsewhere, is explicitly acknowledged in the thesis. I also declare that the intellectual content of this thesis is the product of my own work, except to the extent that assistance from others in the project's design and conception or in style, presentation and linguistic expression is acknowledged.' Signed ..........._. ~ --· .. -: .. ... ... .... .......... ...... Date .... .. .. -~ .T./ _1 _1 / ~Jj /.~ ... .... ........... COPYRIGHT STATEMENT 'I hereby grant the University of New South Wales or its agents the right to archive and to make available my thesis or dissertation in whole or part in the University libraries in all forms of media, now or here after known, subject to the provisions of the Copyright Act 1968. I retain all proprietary rights, such as patent rights. I also retain the right to use in future works (such as articles or books) all or part of this thesis or dissertation.
    [Show full text]
  • A Case Study of Bansi River, Savar, Dhaka, Bangladesh Kamrun Nahar Khan Mukti1
    UITS Journal Volume: 5 Issue: 1 Study of Sources of Pollution and its Effects on Water Quality and Human Habitat: A Case Study of Bansi River, Savar, Dhaka, Bangladesh Kamrun Nahar Khan Mukti1 Abstract—Bansi River catchment is surrounded with different land use activities ranging from urbanization to agriculture and quarrying. The objectives of this study are to establish the number of point and non-point sources pollution of Bansi River and to identify which area source pollutions have the potential to increase the pollution rate in this river with its effects. This study involved both field and laboratory data (secondary data) study. Field study is conducted to establish the number of sources pollution and its effects. The various parameters of the water such as Dissolved Oxygen, Biochemical Oxygen Demand, pH, Chlorine, etc. were examined. Laboratory data was collected from Bangladesh Water Development Board (BWDB) and Department of Environment (DOE). Based on an analysis that has compliance with these standards, it can be concluded that point and non-point sources pollution that toward the station (Nayarhat area) have potential to increase pollution rate in the River. Key words: Point sources pollution, Non-Point source pollution, Water Quality Analysis. Introduction Bansi River is located approximately 20 km far from of the Dhaka city. The Bansi is located within 2393 north latitude to 2381 north latitude and 9021 east longitude to 9026 east longitude. Savar area is on the bank of Eastern side and Nayarhat is on the western side of the Bansi River. The river system of Bansi River is spread in Dhaka district and in neighboring areas of Dhaka City.
    [Show full text]
  • Communicating Science for Conservation
    Communicating Science for Conservation ISSN 0971-6378 (Print); 0973-2543 (Online) Magazine of Zoo Outreach Organization Vol. XXXV, No. 5 www.zoosprint.zooreach.org May 2020 Communicating science for conservation Vol. XXXV, No. 5, May 2020 ISSN 0971-6378 (Print); 0973-2543 (Online) Contents Articles ‘The devil is in the detail’: Peer-review of the Wildlife Conservation Plan by the Wildlife Institute of India for the Etalin Hydropower Project, Dibang Valley -- Chintan Sheth, M. Firoz Ahmed, Sayan Banerjee, Neelesh Dahanukar, Shashank Dalvi, Aparajita Datta, Anirban Datta Roy, Khyanjeet Gogoi, Monsoonjyoti Gogoi, Shantanu Joshi, Arjun Kamdar, Jagdish Krishnaswamy, Manish Kumar, Rohan K. Menzies, Sanjay Molur, Shomita Mukherjee, Rohit Naniwadekar, Sahil Nijhawan, Rajeev Raghavan, Megha Rao, Jayanta Kumar Roy, Narayan Sharma, Anindya Sinha, Umesh Srinivasan, Krishnapriya Tamma, Chihi Umbrey, Nandini Velho, Ashwin Viswanathan & Rameshori Yumnam, Pp. 1–78 Bat Conservation Awareness Campaign: Bats did not directly infect humans with COVID-19 -- Chelmala Srinivasulu, Sanjay Molur, Bhargavi Srinivasulu, Aditya Srinivasulu, Sanjeev Baniya, Pushpa Raj Acharya, Subrat Debata, Harpreet Kaur, Sayantani Nath, Akaanksha Venkataraman, Baheerathan, S., Harshada Yadkikar, Tijo K Joy, Nagarathna, & Vijaya, Pp. 79–91 TidBITS Poem on Corona -- B.R. Arpitha, P. 92 Video on Corona -- Akshaya Pradeep, Rani Pradeep & Pradeep Kumar, P. 92 Articles Sundarvan: An urban green space and its role in supporting wildlife diversity -- S. Sivakumar, Pp. 93–103 Conservation status of wildlife of Bangladesh -- Naim Khandakar & Kamrun Nahar Jeny, Pp. 104–106 Reptile Rap New geographical distribution of Asiatic Softshell Turtle from Mizoram, India -- Gospel Zothanmawia Hmar, Lalmuansanga, Lalbiakzuala, H.T. Lalremsanga & V.L. Mawia, Pp. 107–110 Bugs R All Genetic aberration in a Continental Common Pierrot from West Bengal, India -- Arnob Chakrovorty, Arunava Garai, Banani Bhattacharjee & Asmita Samadder, Pp.
    [Show full text]
  • The Environmentally Conscious Skies: Did the European Union's
    Washington University Global Studies Law Review Volume 14 Issue 1 2015 The Environmentally Conscious Skies: Did the European Union’s Game of Brinksmanship Lead to a Viable Global Plan for Emissions Trading in Aviation? Darren A. Prum Florida State University Kathryn Kisska-Schulze Clemson University Follow this and additional works at: https://openscholarship.wustl.edu/law_globalstudies Part of the Air and Space Law Commons, Commercial Law Commons, Environmental Law Commons, European Law Commons, and the International Law Commons Recommended Citation Darren A. Prum and Kathryn Kisska-Schulze, The Environmentally Conscious Skies: Did the European Union’s Game of Brinksmanship Lead to a Viable Global Plan for Emissions Trading in Aviation?, 14 WASH. U. GLOBAL STUD. L. REV. 1 (2015), https://openscholarship.wustl.edu/law_globalstudies/vol14/iss1/5 This Article is brought to you for free and open access by the Law School at Washington University Open Scholarship. It has been accepted for inclusion in Washington University Global Studies Law Review by an authorized administrator of Washington University Open Scholarship. For more information, please contact [email protected]. Washington University Global Studies Law Review VOLUME 14 NUMBER 1 2015 THE ENVIRONMENTALLY CONSCIOUS SKIES: DID THE EUROPEAN UNION’S GAME OF BRINKSMANSHIP LEAD TO A VIABLE GLOBAL PLAN FOR EMISSIONS TRADING IN AVIATION? DARREN A. PRUM KATHRYN KISSKA-SCHULZE ABSTRACT Effective January 1, 2012, the European Union (EU) instituted the first emissions trading scheme (ETS) for aviation, which affected the domestic and international commercial airlines flying into and out of the EU. The EU established the ETS to counter the global aviation sector’s role in releasing greenhouse gas (GHG) emissions; however, such measures were met with heavy opposition by foreign countries, the International Civil Aviation Organization (ICAO), various commercial airlines and the Air Transport Association of America (ATA).
    [Show full text]
  • Cost Burden of Type 2 Diabetes Mellitus (DM) in an Urban Area of Bangladesh: a Hospital-Based Mix Method Study Running Head: Cost Burden of Type 2 Diabetes Mellitus
    medRxiv preprint doi: https://doi.org/10.1101/2020.08.03.20167478; this version posted August 4, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-ND 4.0 International license . Title: Cost burden of type 2 diabetes mellitus (DM) in an urban area of Bangladesh: A hospital-based mix method study Running Head: Cost Burden of type 2 diabetes mellitus Authors: Monidipa Sahaa, Shirmin Bintay Kadera,b Shafquat Haider Chowdhurya,c, Md. Khaledul Hasanb, Mir Nabila Ashrafd , Md. Marufur Rahmana,e and Kamrun Nahar Kolyb a Department of Public Health, American International University-Bangladesh b Health Systems and Population Studies Division, icddr,b, Dhaka, Bangladesh c Bangladesh institute of health sciences, Dhaka, Bangladesh d Department of Public Health, North South University, Dhaka 1229, Bangladesh e Center for Medical Biotechnology, Directorate General of Health Services, Dhaka 1212, Bangladesh Emails: Monidipa Saha: [email protected] Shirmin Bintay Kader: [email protected] Shafquat Haider Chowdhury: [email protected] Md. Khaledul Hasan: [email protected] Mir Nabila Ashraf: [email protected]; Md. Marufur Rahman: [email protected] Kamrun Nahar Koly: [email protected] Corresponding author: Health Systems and Population Studies Division, icddr,b, Dhaka, Bangladesh [email protected] Word count: Abstract=242; Text=3928 (excluding abstract page, reference, tables and Figures); number of tables=3; number of figures=2 (1 color) Author’s Contributions: All authors contribute significantly to the paper.
    [Show full text]
  • Ricinus Communis L.) - a Biofuel Plant: Morphological and Physiological Parameters Propagated from Seeds in Bangladesh
    Asian Business Review, Volume 2, Numebr 2/2013 (Issue 4) ISSN 2304-2613 (Print); ISSN 2305-8730 (Online) -------------------------------------------------------------------------------------------------------------- Castor Bean (Ricinus communis L.) - A Biofuel Plant: Morphological and Physiological Parameters Propagated from Seeds in Bangladesh Dr. Kamrun Nahar Assistant Professor, Department of Environmental Science and Management, North south University, BANGLADESH ABSTRACT Pot experiments were carried out in Dhaka, Bangladesh from March to September 2012 to evaluate the morphological and physio- logical parameters of Castor oil Plant (Ricinus communis L.), a second generation energy crop propagated from seeds. The leaves and petioles of castor plants were collected from the earthen pot to determine the leaf and petiole nutrient contents. So this study provides a reliable account of the endogenic concentrations of nutrients present in petiole and their content in leaves including the morphological parameters such as plant height, stem diameter, leaf growth, fresh and dry weight of leaves, petiole and root length of the plant at 2 vegetative growth stages grown in Silty clay loam soil were attempted. The experiment revealed that the morpholog- ical parameters responded better in mature plant compared to young plant but the physiological parameter showed variations at 2 growth stages. Key words: Castor oil plant, Ricinus communis, Height, leaf, Root and Nutrient uptake. 1 INTRODUCTION astor oil plant (Ricinus communis L.) is a non food, The Plant is produced from seed as well as by tissue cul- C drought resistant, energy crop gaining attention for ture. Regeneration by tissue culture technique would also producing biofuel as biodiesel in developed as well as be a feasible alternative for improving the quality and pro- in developing countries.
    [Show full text]
  • A Morphological and Physiological Study of Jatropha Curcas Linn
    Middle-East Journal of Scientific Research 13 (8): 1115-1118, 2013 ISSN 1990-9233 © IDOSI Publications, 2013 DOI: 10.5829/idosi.mejsr.2013.13.8.623 A Morphological and Physiological Study of Jatropha curcas Linn. Propagated from Seeds in Bangladesh 12Kamrun Nahar and Sirajul Hoque 1North South University, Dhaka, Bangladesh 2University of Dhaka, Bangladesh Abstract: Pot experiments were carried out in Dhaka, Bangladesh to evaluate the morphological and physiological parameters of Jatropha, a second generation energy crop propagated from seeds. The leaves and petioles of Jatropha curcas plants were collected from the earthen pot to determine the leaf and petiole nutrient contents. So this study provides a reliable account of the endogenic concentrations of nutrients present in petiole and their content in leaves including the morphological parameters such as plant height, leaf growth, fresh and dry weight of leaves, petiole and root length of the plant at 2 vegetative growth stages grown in Silty clay loam soil were attempted. The experiment revealed that the morphological parameters responded better in mature plant compared to young plant but the physiological parameter showed variations at 2 growth stages. Key words: Jatropha curcas Height Leaf Root length and Nutrient uptake INTRODUCTION production [2,3]. As an oil bearing biomass feedstock, it can ensure an alternative source of energy and reduce our In recent years public attention has been focused on dependency on fossil fuel. This plant can grow anywhere renewable sources of energy in both developed and including soil considered infertile for food production and developing countries all over the world. Biofuel, which is can live for about 50 years [4].
    [Show full text]
  • Jatropha: an Alternative Substitute to Fossil Fuel1 Kamrun Nahar and Monica Ozores-Hampton2
    Archival copy: for current recommendations see http://edis.ifas.ufl.edu or your local extension office. HS1193 Jatropha: An Alternative Substitute to Fossil Fuel1 Kamrun Nahar and Monica Ozores-Hampton2 Abstract second-generation biofuel is to increase the biofuel supply with crops such as Jatropha, castor (Ricinus communis), and Jatropha curcas L. is a non-food bioenergy plant that is Camelina (Camelina sativa). Jatropha yields a considerable known for the production of biofuel. Jatropha can be con- amount of inedible oil that can be converted to biodiesel. sidered a second-generation biofuel plant that may provide Th e oil can be used as a direct replacement for fuel in a portion of the fuel supply. Jatropha is a tropical plant and engines and machines, and it has other industrial and com- can be grown in low to high rainfall and diverse soil types, mercial uses as well (Cerrate et al. 2006; Ndong et al. 2009). but the plant is susceptible to freezes. Th e plant produces Additionally, diff erent parts of Jatropha have medicinal seeds containing inedible oil that can be converted to bio- value, such as anticancer properties (Duke 1983). Roots diesel, which can be used in the transportation and energy and leaves can be used to make antibiotics and products sectors. Th e detoxifi ed cake by-product from oil extraction for the treatment of skin diseases (Henning 2002). Being can be used for fi sh and animal feed, biogas, or as an rich in nitrogen (N), the seed cake can be an excellent plant organic fertilizer. Th e crop can be mechanically harvested, nutrient source if detoxifi ed (Makkar et al.
    [Show full text]