bioRxiv preprint doi: https://doi.org/10.1101/329938; this version posted May 24, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 CD32+ and PD-1+ Lymph Node CD4 T Cells Support Persistent HIV-1 2 Transcription in Treated Aviremic Individuals 3 Alessandra Notoa, Francesco A. Procopioa, Riddhima Bangaa, Madeleine Suffiottia, Jean-Marc 4 Corpatauxb, Matthias Cavassinic, Craig Fenwicka, Raphael Gottardod, Matthieu Perreaua, 5 Giuseppe Pantaleoa,e# 6 7 aService of Immunology and Allergy, Lausanne University Hospital, University of Lausanne, 8 Lausanne, Switzerland 9 bService of Vascular Surgery, Lausanne University Hospital, University of Lausanne, Lausanne, 10 Switzerland 11 cService of Infectious Diseases, Lausanne University Hospital, University of Lausanne, 12 Lausanne, Switzerland 13 dVaccine and Infectious Disease Divisions, Fred Hutchinson Cancer Research Center, Seattle, 14 Washington, USA 15 eSwiss Vaccine Research Institute, Lausanne University Hospital, University of Lausanne, 16 Lausanne, Switzerland 17 Running Head: Role of CD32 and PD-1 in Defining the HIV Reservoir 18 #Address correspondence to Giuseppe Pantaleo,
[email protected] 19 Word count for the abstract: 318 Word count for the text: 4130 20 1 bioRxiv preprint doi: https://doi.org/10.1101/329938; this version posted May 24, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 21 ABSTRACT 22 A recent study conducted in blood has proposed CD32 as the marker identifying the ‘elusive’ HIV 23 reservoir. We have investigated the distribution of CD32+ CD4 T cells in blood and lymph nodes 24 (LNs) of healthy HIV-1 uninfected, viremic untreated and long-term treated HIV-1 infected 25 individuals and their relationship with PD-1+ CD4 T cells.