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Effects of Spaceflight and Simulated Microgravity on Microbial Growth and Secondary Metabolism Bing Huang1†, Dian-Geng Li1†, Ying Huang2* and Chang-Ting Liu1*

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Effects of Spaceflight and Simulated Microgravity on Microbial Growth and Secondary Metabolism Bing Huang1†, Dian-Geng Li1†, Ying Huang2* and Chang-Ting Liu1* Huang et al. Military Medical Research (2018) 5:18 https://doi.org/10.1186/s40779-018-0162-9 REVIEW Open Access Effects of spaceflight and simulated microgravity on microbial growth and secondary metabolism Bing Huang1†, Dian-Geng Li1†, Ying Huang2* and Chang-Ting Liu1* Abstract Spaceflight and ground-based microgravity analog experiments have suggested that microgravity can affect microbial growth and metabolism. Although the effects of microgravity and its analogs on microorganisms have been studied for more than 50 years, plausible conflicting and diverse results have frequently been reported in different experiments, especially regarding microbial growth and secondary metabolism. Until now, only the responses of a few typical microbes to microgravity have been investigated; systematic studies of the genetic and phenotypic responses of these microorganisms to microgravity in space are still insufficient due to technological and logistical hurdles. The use of different test strains and secondary metabolites in these studies appears to have caused diverse and conflicting results. Moreover, subtle changes in the extracellular microenvironments around microbial cells play a key role in the diverse responses of microbial growth and secondary metabolisms. Therefore, “indirect” effects represent a reasonable pathway to explain the occurrence of these phenomena in microorganisms. This review summarizes current knowledge on the changes in microbial growth and secondary metabolism in response to spaceflight and its analogs and discusses the diverse and conflicting results. In addition, recommendations are given for future studies on the effects of microgravity in space on microbial growth and secondary metabolism. Keywords: Microbial growth, Secondary metabolism, Spaceflight, Microgravity, Simulated microgravity, Microgravity analogs Background [21–23], and microbial mutations and relation to adapta- Microbes are highly evolved [1] and can survive in tion to LSMMG [24]. Considerable effort has been fo- many extreme environments [2, 3], including outer cused on cell growth and secondary metabolism. space [4, 5]. However, the different mechanisms by The significance of exploring the effects of space which they respond and adapt to these environments microgravity on microbial growth and metabolism in- (especially to microgravity in space) remain unclear. Re- cludes two important implications. First, the growth of cently, spaceflight and ground simulated microgravity microorganisms (especially pathogenic microbes) in a (SMG) or low-shear modeled microgravity (LSMMG) ex- space capsule could be a threat to astronaut health and periments have demonstrated that microgravity can affect be detrimental to their immune systems [10, 11, 25, 26]. cellular processes and functions in microorganisms, such Second, microorganisms can produce many special sec- as cell growth [6–9], gene expression [10–12], cell morph- ondary metabolites that could be utilized as medicine ology and development [13, 14], virulence and resistance for both humans and animals [5, 23, 27] as well as some [15–18], biofilm formation [19, 20], secondary metabolism toxic secondary metabolites that may threaten the health of astronauts [28]. Investigations into whether the pro- duction of secondary metabolites by these microorganisms * Correspondence: [email protected]; [email protected] is altered in the space environment are worthwhile. †Bing Huang and Dian-Geng Li contributed equally to this work. 2State Key Laboratory of Microbial Resources, Institute of Microbiology, Although studies on the responses of microbes to Chinese Academy of Sciences, Beijing 100101, China microgravity date back to the 1960s, many basic ques- 1Nanlou Respiratory Diseases Department, Chinese PLA General Hospital/ tions concerning the effects of microgravity on microbial Chinese PLA Postgraduate Medical School, Beijing 100853, China © The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. Huang et al. Military Medical Research (2018) 5:18 Page 2 of 14 behavior are far from being fully resolved [29, 30]. More- level (i.e., the value of equivalent accelerated speed) over, our systematic and in-depth understanding of the needs to be specified in this context. Generally, the − genetic and phenotypic responses of a variety of micro- “microgravity” level ranges from approximately 10 3 to − organisms to microgravity environments in space is in- 10 6 g and is dependent on the location within the sufficient due to technological and logistical hurdles. To spacecraft and the frequency of vibrations [33, 34]. date, only a few typical microbes, including streptomy- Therefore, the term “microgravity” has been suggested cetes, have been investigated in terms of their responses to be exclusively restricted to experiments performed in to microgravity and its analogs [29]. Interestingly, plaus- an environment such as drop towers, parabolic flights, ible but conflicting results for cellular growth rates were sounding rockets, recoverable satellites, spaceships, and reported in different spaceflight and clinorotation exper- the space station (spacelab). Furthermore, the usage of iments [31]. In addition to the microbial growth rate, the term “microgravity” should be independent of the secondary metabolism was also found to be similarly sen- interfering factors of the actual acceleration of the sitive to microgravity and simulated microgravity [22, 31]. spacecraft in space and cosmic radiation, while the term Furthermore, the results of these studies have been mixed, “spaceflight” should contain “microgravity” and the other without conclusive assertions and suggestions for future inherent factors in space (i.e., cosmic radiation). antibiotic production in space environments [22, 32]. Generally, each type of spaceflight opportunity has its Thus, nothing conclusive or concrete is known about the own time range of duration and corresponding “micro- effects of microgravity or simulated microgravity on mi- gravity” level based on the various spaceflight technolo- crobial growth and secondary metabolism; thus, this area gies [35] (Table 1). To date, many studies associated of research remains open to further exploration. with the responses of terrestrial life have been conducted In this review, we compare the technological methods in space microgravity conditions by recoverable satellites, of microgravity experiments used for spaceflight and space shuttles and on the space station (spaceship) [4]. ground-based simulated microgravity. We also analyzed The effect of an organism in response to the microgravity the similarities and differences in their effects on micro- of a space experiment in these studies is frequently de- bial growth and secondary metabolism as well as the scribed as the “spaceflight effect” due to considerations of causes of the inconsistent results. Based on the analysis the interference of cosmic radiation, spacecraft vibrations of previous studies, it is clear that the experiments per- and hypervelocity; the effects of microgravity and space- formed under spaceflight and SMG conditions differed flight are different. Earlier studies often lacked on board in some procedures, including in the use of different controls during spaceflight due to restrictions in the use strains, growth media, and types of ground-based facilities of centrifuges and sample fixation in orbit. Recently, these (GBFs), which may lead to conflicting results. We also drawbacks have been gradually overcome by using an propose that subtle differences in the microenvironment incubator-centrifuge in orbit that could simulate 1 g Earth could play a key role in the diverse responses observed for gravity and thus separate other space environmental fac- microbial growth and secondary metabolism. Finally, we tors during spaceflight. Furthermore, real-time sample fix- provide recommendations for future studies on the effects ation in orbit could avoid the interference of spacecraft of microgravity in a space environment on microbial landing [36, 37]. growth and secondary metabolism. Although some of these studies were conducted in a space environment by means of the spacecraft and space Space microgravity and its analogs on the ground station, microgravity experiments in space are costly and A large proportion of the experiments were performed performed infrequently due to technological and logis- under simulated microgravity conditions using ground- tical hurdles. Hence, several GBFs with different physical based microgravity simulators due to the scarcity and cost- concepts have been constructed to simulate microgravity liness of spaceflight opportunities. However, it should be on the ground [38, 39] (Table 2). The term “simulated noted that the real microgravity in space is not equivalent to microgravity analogs using ground-based simulators. Table 1 Several flight opportunities and their characteristics Therefore, questions remain concerning the similarities Flight opportunities Time of duration
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