C146-E227A LC/MS/MS Method Package for Primary Metabolites

C146-E227A

LC/MS/MS Method Package for Primary Metabolites Ver. 2 For LabSolutions Ver. 5

LCMS-8050

Ready-to-Use Analytical Conditions Complete from Sample Preparation to Analysis Shimadzu Method Packages deliver conditions for efﬁcient and simultaneous Protocols are included for the preparation of extracts from biological tissue. multi-component analysis. They enable the user to quickly and easily implement Saving the user time and money, even laboratories unfamiliar with extraction complex methods without costly and laborious method development by can follow prescribed steps for LC/MS/MS sample preparation. providing sample preparation protocols, LC separation conditions, and MS acquisition parameters. Normalization of Multiple Sample Results Ion pair or PFPP column method Due to mass differences between biological tissue samples, normalization of results must be performed when multiple samples are analyzed. This method A PFPP:Pentaﬂuorophenylpropyl column method was newly adapted to expand your package includes optimized analytical conditions for two internal standards choice in separation conditions as well as target compounds. to permit normalization across multiple samples.

Supporting LCMS-8030/8040/8050

1 2 This method package supports the latest Shimadzu UFMS triple quadrupole mass S-Adenosylmethionine spectrometer series, LCMS-8030, 8040 and 8050. LCMS-8050 delivers best-in-class 1 2 1 2 sensitivity and the fastest scanning and polarity switching rates. Methionine S-Adenosylhomocysteine

60000 55 STDs Mix. Tyrosine Glucose-6-phosphate Erythrose-4-phosphate TMP ADP 1 2 55000 MES (2-Morpholinoethanesulfonic acid) Ribose-5-phosphate Ribulose-5-phosphate AMP TDP Homocysteine Glutamate Sedoheptulose-7-phosphate Pyruvate cGMP Phosphoenolpyruvate Adenosine 1 2 50000 Aspartate Fructose-6-phosphate CMP cAMP CTP Cystathionine Phenylalanine Tryptophan NAD CDP GTP 1 2 45000 Glycerol-3-phosphate Dihydroxyacetonephosphate 6-Phosphogluconate UTP Inosine Glucose-1-phosphate UMP GDP ATP 40000 Cysteine Glyceraldehyde-3-phosphate GMP UDP TTP 1 2 Uric acid 35000 3-Phosphoglycerate NADPH 1 2 /2-Phoshoglycerate 2,3-bis-Phosphoglycerate 5-Glutamylcysteine 30000 NADP Succinyl-CoA Fructose-1,6-bis- Acetyl-CoA 1 2 25000 phosphate 1 2 Glutathione NADH 20000 Lysine Alanine 1 2 15000 Arginine Glutamine Oxidized glutathione 10000 Histidine Threonine Glycine Cysteine 5000 Serine L-Methionine sulfone Asparagine Methionine 0 1 2 0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0 min Area ratio comparison between two tissues focused Overlaid MRM chromatograms for 55 standards mixture by ion pair method on methylation cycle LC/MS/MS Method Package for Primary Metabolites Ver. 2 For LabSolutions Ver. 5

Index of compounds List of compounds for ion pair method List of compounds for PFPP column method Glycolytic system Nucleotides system lytic Glyco- Organic acids 2,3-Bisphosphoglyceric acid Adenosine 3',5'-cyclic monophosphate Lactic acid 4-Aminobutyric acid 3-Phosphoglyceric acid Adenosine diphosphate Pyruvic acid Adenylsuccinic acid

(2-Phosphoglyceric acid) Adenosine monophosphate TCA cycle Argininosuccinic acid Dihydroxyacetone phosphate Adenosine triphosphate 2-Ketoglutaric acid Cholic acid Fructose 1,6-bisphosphate Cytidine diphosphate Aconitic acid Creatine Glucose 1-phosphate Cytidine monophosphate Citric acid Nicotinic acid Glucose 6-phosphate Cytidine triphosphate Fumaric acid Ophthalmic acid Glycerol 3-phosphate Guanosine 3',5'-cyclic monophosphate Isocitric acid Orotic acid Phosphoenolpyruvic acid Guanosine diphosphate Malic acid Pantothenic acid Pyruvic acid Guanosine monophosphate Succinic acid Taurocholic acid pathway Pentose-phosphate Fructose 6-phosphate Guanosine triphosphate Amino acids Uric acid 4-Hydroxyproline

Glyceraldehyde 3-phosphate Thymidine diphosphate Nucleosides and Nucleotides Alanine 6-Phosphogluconic acid Thymidine monophosphate Adenine Arginine Erythrose 4-phosphate Thymidine triphosphate Cytosine Asparagine Ribose 5-phosphate Uridine diphosphate Guanine Aspartic acid Ribulose 5-phosphate Uridine monophosphate Thymine Asymmetric dimethylarginine Sedoheptulose 7-phosphate Uridine triphosphate Uracil Citrulline Xanthine cycle TCA Co-enzyme Acetyl coenzyme A NAD Cystine Adenosine Succinyl coenzyme A NADH Dimethylglycine Cytidine Glutamic acid Amino acids NADP Guanosine Alanine NADPH Glutamine Inosine Arginine Glycine STDs nal Inter- Thymidine Asparagine 2-Morpholinoethanesulfonic acid Histidine Uridine Aspartic acid Methionine sulfone Homocystine Adenosine 3',5'-cyclic monophosphate Cysteine Isoleucine Adenosine monophosphate Glutamic acid Leucine Cytidine 3',5'-cyclic monophosphate Glutamine Lysine Cytidine monophosphate Glycine Methionine sulfoxide Guanosine 3',5'-cyclic monophosphate Histidine Ornitine Guanosine monophosphate Lysine Phenylalanine Thymidine monophosphate Methionine Proline enzymes Co- Phenylalanine Serine FAD Serine Symmetric dimethylarginine FMN Threonine Threonine NAD

Tryptophan Tryptophan Others Tyrosine Tyrosine 2-Aminobutyric acid Valine Acetylcarnitine Transsulfuration cycle Methylation and Cystathionine Acetylcholine Cysteine Allantoin * The ion pair method is suitable for metabolites associated with a glycolytic system, Homocysteine Carnitine pentose-phosphate pathway and either ADP or ATP. Methionine Carnosine 5-Glutamylcysteine Choline * The PFPP column method is good for covering amino acids, organic acids and metabolites Glutathione Citicoline related to the methylation cycle and urea cycle. Oxidized glutathione Creatinine S-Adenosylhomocysteine Cysteamine S-Adenosylmethionine Dopa Dopamine Epinephrine Histamine Hypoxanthine Kynurenine Niacinamide Norepinephrine Serotonin STDs nal Inter- Remarks and Precautions 2-Morpholinoethanesulfonic acid Methionine sulfone 1. For Research Use Only. Not for Use in Diagnostic Procedures. 2. Shimadzu makes no warranty regarding the accuracy of information included in the database or the usefulness of information obtained from using the database. 3. It is the user’s responsibility to adopt appropriate quality control tests using standard samples to conﬁrm qualitative and quantitative information obtained with this method package.