Cannabidiol Protects Dopaminergic Neuronal Cells from Cadmium
International Journal of Environmental Research and Public Health Article Cannabidiol Protects Dopaminergic Neuronal Cells from Cadmium Jacopo Junio Valerio Branca 1,* , Gabriele Morucci 1,*, Matteo Becatti 2 , Donatello Carrino 1, Carla Ghelardini 3, Massimo Gulisano 1, Lorenzo Di Cesare Mannelli 3 and Alessandra Pacini 1 1 Department of Experimental and Clinical Medicine, Histology and Anatomy Section, University of Firenze, 50134 Firenze, Italy; donatello.carrino@unifi.it (D.C.); massimo.gulisano@unifi.it (M.G.); alessandra.pacini@unifi.it (A.P.) 2 Department of Experimental and Clinical Biomedical Sciences “Mario Serio”, University of Firenze, 50134 Firenze, Italy; matteo.becatti@unifi.it 3 Department of Neuroscience, Psychology, Drug Research and Child Health (NEUROFARBA), Pharmacology and Toxicology Section, University of Firenze, 50139 Firenze, Italy; carla.ghelardini@unifi.it (C.G.); lorenzo.mannelli@unifi.it (L.D.C.M.) * Correspondence: jacopojuniovalerio.branca@unifi.it (J.J.V.B.); gabriele.morucci@unifi.it (G.M.); Tel.: +39-055-2758067 (J.J.V.B.) Received: 21 October 2019; Accepted: 5 November 2019; Published: 12 November 2019 Abstract: The protective effect of cannabidiol (CBD), the non-psychoactive component of Cannabis sativa, against neuronal toxicity induced by cadmium chloride (CdCl2 10 µM) was investigated in a retinoic acid (RA)-differentiated SH-SY5Y neuroblastoma cell line. CBD (1 µM) was applied 24 h before and removed during cadmium (Cd) treatment. In differentiated neuronal cells, CBD significantly reduced the Cd-dependent decrease of cell viability, and the rapid reactive oxygen species (ROS) increase. CBD significantly prevented the endoplasmic reticulum (ER) stress (GRP78 increase) and the subcellular distribution of the cytochrome C, as well as the overexpression of the pro-apoptotic protein BAX.
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