Bioengineering Coagulation Factor Xa Substrate Specificity Into
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2-Diss Preface1
Purification and preliminary characterization of Bothrops moojeni venom components active on haemostasis (Botmo Thesis) Inauguraldissertation zur Erlangung der Würde eines Doktors der Philosophie vorgelegt der Philosophisch-Naturwissenschaftlichen Fakultät der Universität Basel von Anna Maria Perchu ć aus Warschau, Polen Referent: Prof. Dr. Beat Ernst Korreferent: Prof. Dr. phil. Jürg Meier Basel, 2010 Genehmigt von der Philosophisch-Naturwissenschaftlichen Fakultät auf Antrag von Herrn Prof. Dr. Beat Ernst und Herrn Prof. Dr. phil. Jürg Meier Basel, den 16. September 2008 Prof. Dr. Eberhard Parlow Dekan Wissenschaft ist der gegenwärtige Stand unseres Irrtums Jakob Franz Kern (1897-1924) Anna Maria Perchuć – Botmo Thesis I Acknowledgements To my Doktorvater Prof. Dr. Beat Ernst for his patience, support and scientific advice. To Prof. Dr. phil. Jürg Meier (my Korreferent) and to Prof. Dr. Reto Brun for their input to my PhD exam. To Pentaphatm Ltd. for giving me the opportunity to work on the “Bothrops moojeni Venom Proteomics Project”. To Marianne and Bea for helpful discussions, scientific, practical and personal support, their enthusiasm and friendship. To Reto for giving me the encouragement and guidance and for coordinating the Botmo Project. To the whole Hämostase und Test-Kit-Entwicklung Team for their friendly and helpful cooperation. To Laure, Reto, Philou and the whole Atheris Team for their scientific assistance. To Marc and his Synthesis Team for their involvement in the Botmo Project, their help, support and sense of humour. To Uwe and Andre for the fruitful cooperation. To Andreas for his great assistance and many valuable suggestions. To Remo and Martin for their support in the cell culture lab. -
Serine Proteases with Altered Sensitivity to Activity-Modulating
(19) & (11) EP 2 045 321 A2 (12) EUROPEAN PATENT APPLICATION (43) Date of publication: (51) Int Cl.: 08.04.2009 Bulletin 2009/15 C12N 9/00 (2006.01) C12N 15/00 (2006.01) C12Q 1/37 (2006.01) (21) Application number: 09150549.5 (22) Date of filing: 26.05.2006 (84) Designated Contracting States: • Haupts, Ulrich AT BE BG CH CY CZ DE DK EE ES FI FR GB GR 51519 Odenthal (DE) HU IE IS IT LI LT LU LV MC NL PL PT RO SE SI • Coco, Wayne SK TR 50737 Köln (DE) •Tebbe, Jan (30) Priority: 27.05.2005 EP 05104543 50733 Köln (DE) • Votsmeier, Christian (62) Document number(s) of the earlier application(s) in 50259 Pulheim (DE) accordance with Art. 76 EPC: • Scheidig, Andreas 06763303.2 / 1 883 696 50823 Köln (DE) (71) Applicant: Direvo Biotech AG (74) Representative: von Kreisler Selting Werner 50829 Köln (DE) Patentanwälte P.O. Box 10 22 41 (72) Inventors: 50462 Köln (DE) • Koltermann, André 82057 Icking (DE) Remarks: • Kettling, Ulrich This application was filed on 14-01-2009 as a 81477 München (DE) divisional application to the application mentioned under INID code 62. (54) Serine proteases with altered sensitivity to activity-modulating substances (57) The present invention provides variants of ser- screening of the library in the presence of one or several ine proteases of the S1 class with altered sensitivity to activity-modulating substances, selection of variants with one or more activity-modulating substances. A method altered sensitivity to one or several activity-modulating for the generation of such proteases is disclosed, com- substances and isolation of those polynucleotide se- prising the provision of a protease library encoding poly- quences that encode for the selected variants. -
Chrystelle Bonnart
UNIVERSITE TOULOUSE III – PAUL SABATIER U.F.R. Sciences T H E S E Pour obtenir le grade de DOCTEUR DE L’UNIVERSITE TOULOUSE III Discipline : Physiopathologie moléculaire, cellulaire et intégrée Présentée et soutenue par Chrystelle Bonnart Le 20 novembre 2007 Etude fonctionnelle de LEKTI et de sa nouvelle cible, l’élastase 2 pancréatique Directeur de thèse : Pr Alain Hovnanian JURY Pr Alain Hovnanian, Président Pr Pierre Dubus, rapporteur Pr Michèle Reboud-Ravaux, rapporteur Dr Heather Etchevers, examinateur 2 Heureux sont les fêlés, car ils laissent passer la lumière… Michel Audiard 3 REMERCIEMENTS Tout d’abord, mes remerciements s’adressent à mon directeur de thèse, Alain Hovnanian, pour m’avoir accueillie dans son équipe, pour m’avoir rapidement fait confiance en me laissant une grande autonomie et pour m’avoir encouragée à participer à des congrès internationaux. Je tiens à remercier Pr Pierre Dubus et Pr Michèle Reboud-Ravaux pour m’avoir fait l’honneur d’évaluer ce travail de thèse. Je remercie également Dr Heather Etchevers pour sa présence dans le jury de thèse. Je remercie vivement les enseignant-chercheurs et professeurs de l’UPS que j’ai eu l’occasion de croiser pendant mon monitorat. Tout d’abord, merci à Estelle Espinos, tu as assumé ton rôle de tutrice avec brio, tu as été à mes côtés dans mes débuts hésitants, merci pour ta disponibilité. Merci à Martine Briet et Pascale Bélenguer, qui m’ont fait bénéficier de leur grande expérience de l’enseignement. Un merci particulier à Nathalie Ortega, que j’ai eu la chance de rencontrer dans ma dernière année de monitorat. -
Durham E-Theses
Durham E-Theses Midgut proteases from larval spodoptera littoralis (lepidoptera: noctutoae) Lee, Michael James How to cite: Lee, Michael James (1992) Midgut proteases from larval spodoptera littoralis (lepidoptera: noctutoae), Durham theses, Durham University. Available at Durham E-Theses Online: http://etheses.dur.ac.uk/5739/ Use policy The full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-prot purposes provided that: • a full bibliographic reference is made to the original source • a link is made to the metadata record in Durham E-Theses • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders. Please consult the full Durham E-Theses policy for further details. Academic Support Oce, Durham University, University Oce, Old Elvet, Durham DH1 3HP e-mail: [email protected] Tel: +44 0191 334 6107 http://etheses.dur.ac.uk MIDGUT PROTEASES FROM LARVAL SPODOPTERA LITTORALIS (LEPIDOPTERA: NOCTUTOAE) By Michael James Lee B.Sc. (Dunelm) The copyright of this thesis rests with the author. No quotation from it should be pubhshed without his prior written consent and information derived from it should be acknowledged. Being a thesis submitted for the degree of Doctor of Philosophy of the University of Durham. November, 1992 Hatfield College University of Durham 6 APR 1993 DECLARATION I hereby declare that the work presented in this document is based on research carried out by me, and that no part has been previously submitted for a degree in this or any other university. -
Manual D'estil Per a Les Ciències De Laboratori Clínic
MANUAL D’ESTIL PER A LES CIÈNCIES DE LABORATORI CLÍNIC Segona edició Preparada per: XAVIER FUENTES I ARDERIU JAUME MIRÓ I BALAGUÉ JOAN NICOLAU I COSTA Barcelona, 14 d’octubre de 2011 1 Índex Pròleg Introducció 1 Criteris generals de redacció 1.1 Llenguatge no discriminatori per raó de sexe 1.2 Llenguatge no discriminatori per raó de titulació o d’àmbit professional 1.3 Llenguatge no discriminatori per raó d'ètnia 2 Criteris gramaticals 2.1 Criteris sintàctics 2.1.1 Les conjuncions 2.2 Criteris morfològics 2.2.1 Els articles 2.2.2 Els pronoms 2.2.3 Els noms comuns 2.2.4 Els noms propis 2.2.4.1 Els antropònims 2.2.4.2 Els noms de les espècies biològiques 2.2.4.3 Els topònims 2.2.4.4 Les marques registrades i els noms comercials 2.2.5 Els adjectius 2.2.6 El nombre 2.2.7 El gènere 2.2.8 Els verbs 2.2.8.1 Les formes perifràstiques 2.2.8.2 L’ús dels infinitius ser i ésser 2.2.8.3 Els verbs fer, realitzar i efectuar 2.2.8.4 Les formes i l’ús del gerundi 2.2.8.5 L'ús del verb haver 2.2.8.6 Els verbs haver i caldre 2.2.8.7 La forma es i se davant dels verbs 2.2.9 Els adverbis 2.2.10 Les locucions 2.2.11 Les preposicions 2.2.12 Els prefixos 2.2.13 Els sufixos 2.2.14 Els signes de puntuació i altres signes ortogràfics auxiliars 2.2.14.1 La coma 2.2.14.2 El punt i coma 2.2.14.3 El punt 2.2.14.4 Els dos punts 2.2.14.5 Els punts suspensius 2.2.14.6 El guionet 2.2.14.7 El guió 2.2.14.8 El punt i guió 2.2.14.9 L’apòstrof 2.2.14.10 L’interrogant 2 2.2.14.11 L’exclamació 2.2.14.12 Les cometes 2.2.14.13 Els parèntesis 2.2.14.14 Els claudàtors 2.2.14.15 -
(12) Patent Application Publication (10) Pub. No.: US 2004/0081648A1 Afeyan Et Al
US 2004.008 1648A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2004/0081648A1 Afeyan et al. (43) Pub. Date: Apr. 29, 2004 (54) ADZYMES AND USES THEREOF Publication Classification (76) Inventors: Noubar B. Afeyan, Lexington, MA (51) Int. Cl." ............................. A61K 38/48; C12N 9/64 (US); Frank D. Lee, Chestnut Hill, MA (52) U.S. Cl. ......................................... 424/94.63; 435/226 (US); Gordon G. Wong, Brookline, MA (US); Ruchira Das Gupta, Auburndale, MA (US); Brian Baynes, (57) ABSTRACT Somerville, MA (US) Disclosed is a family of novel protein constructs, useful as Correspondence Address: drugs and for other purposes, termed “adzymes, comprising ROPES & GRAY LLP an address moiety and a catalytic domain. In Some types of disclosed adzymes, the address binds with a binding site on ONE INTERNATIONAL PLACE or in functional proximity to a targeted biomolecule, e.g., an BOSTON, MA 02110-2624 (US) extracellular targeted biomolecule, and is disposed adjacent (21) Appl. No.: 10/650,592 the catalytic domain So that its affinity Serves to confer a new Specificity to the catalytic domain by increasing the effective (22) Filed: Aug. 27, 2003 local concentration of the target in the vicinity of the catalytic domain. The present invention also provides phar Related U.S. Application Data maceutical compositions comprising these adzymes, meth ods of making adzymes, DNA's encoding adzymes or parts (60) Provisional application No. 60/406,517, filed on Aug. thereof, and methods of using adzymes, Such as for treating 27, 2002. Provisional application No. 60/423,754, human Subjects Suffering from a disease, Such as a disease filed on Nov. -
A Genomic Analysis of Rat Proteases and Protease Inhibitors
A genomic analysis of rat proteases and protease inhibitors Xose S. Puente and Carlos López-Otín Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Instituto Universitario de Oncología, Universidad de Oviedo, 33006-Oviedo, Spain Send correspondence to: Carlos López-Otín Departamento de Bioquímica y Biología Molecular Facultad de Medicina, Universidad de Oviedo 33006 Oviedo-SPAIN Tel. 34-985-104201; Fax: 34-985-103564 E-mail: [email protected] Proteases perform fundamental roles in multiple biological processes and are associated with a growing number of pathological conditions that involve abnormal or deficient functions of these enzymes. The availability of the rat genome sequence has opened the possibility to perform a global analysis of the complete protease repertoire or degradome of this model organism. The rat degradome consists of at least 626 proteases and homologs, which are distributed into five catalytic classes: 24 aspartic, 160 cysteine, 192 metallo, 221 serine, and 29 threonine proteases. Overall, this distribution is similar to that of the mouse degradome, but significatively more complex than that corresponding to the human degradome composed of 561 proteases and homologs. This increased complexity of the rat protease complement mainly derives from the expansion of several gene families including placental cathepsins, testases, kallikreins and hematopoietic serine proteases, involved in reproductive or immunological functions. These protease families have also evolved differently in the rat and mouse genomes and may contribute to explain some functional differences between these two closely related species. Likewise, genomic analysis of rat protease inhibitors has shown some differences with the mouse protease inhibitor complement and the marked expansion of families of cysteine and serine protease inhibitors in rat and mouse with respect to human. -
12) United States Patent (10
US007635572B2 (12) UnitedO States Patent (10) Patent No.: US 7,635,572 B2 Zhou et al. (45) Date of Patent: Dec. 22, 2009 (54) METHODS FOR CONDUCTING ASSAYS FOR 5,506,121 A 4/1996 Skerra et al. ENZYME ACTIVITY ON PROTEIN 5,510,270 A 4/1996 Fodor et al. MICROARRAYS 5,512,492 A 4/1996 Herron et al. 5,516,635 A 5/1996 Ekins et al. (75) Inventors: Fang X. Zhou, New Haven, CT (US); 5,532,128 A 7/1996 Eggers Barry Schweitzer, Cheshire, CT (US) 5,538,897 A 7/1996 Yates, III et al. s s 5,541,070 A 7/1996 Kauvar (73) Assignee: Life Technologies Corporation, .. S.E. al Carlsbad, CA (US) 5,585,069 A 12/1996 Zanzucchi et al. 5,585,639 A 12/1996 Dorsel et al. (*) Notice: Subject to any disclaimer, the term of this 5,593,838 A 1/1997 Zanzucchi et al. patent is extended or adjusted under 35 5,605,662 A 2f1997 Heller et al. U.S.C. 154(b) by 0 days. 5,620,850 A 4/1997 Bamdad et al. 5,624,711 A 4/1997 Sundberg et al. (21) Appl. No.: 10/865,431 5,627,369 A 5/1997 Vestal et al. 5,629,213 A 5/1997 Kornguth et al. (22) Filed: Jun. 9, 2004 (Continued) (65) Prior Publication Data FOREIGN PATENT DOCUMENTS US 2005/O118665 A1 Jun. 2, 2005 EP 596421 10, 1993 EP 0619321 12/1994 (51) Int. Cl. EP O664452 7, 1995 CI2O 1/50 (2006.01) EP O818467 1, 1998 (52) U.S. -
(12) United States Patent (10) Patent No.: US 8,561,811 B2 Bluchel Et Al
USOO8561811 B2 (12) United States Patent (10) Patent No.: US 8,561,811 B2 Bluchel et al. (45) Date of Patent: Oct. 22, 2013 (54) SUBSTRATE FOR IMMOBILIZING (56) References Cited FUNCTIONAL SUBSTANCES AND METHOD FOR PREPARING THE SAME U.S. PATENT DOCUMENTS 3,952,053 A 4, 1976 Brown, Jr. et al. (71) Applicants: Christian Gert Bluchel, Singapore 4.415,663 A 1 1/1983 Symon et al. (SG); Yanmei Wang, Singapore (SG) 4,576,928 A 3, 1986 Tani et al. 4.915,839 A 4, 1990 Marinaccio et al. (72) Inventors: Christian Gert Bluchel, Singapore 6,946,527 B2 9, 2005 Lemke et al. (SG); Yanmei Wang, Singapore (SG) FOREIGN PATENT DOCUMENTS (73) Assignee: Temasek Polytechnic, Singapore (SG) CN 101596422 A 12/2009 JP 2253813 A 10, 1990 (*) Notice: Subject to any disclaimer, the term of this JP 2258006 A 10, 1990 patent is extended or adjusted under 35 WO O2O2585 A2 1, 2002 U.S.C. 154(b) by 0 days. OTHER PUBLICATIONS (21) Appl. No.: 13/837,254 Inaternational Search Report for PCT/SG2011/000069 mailing date (22) Filed: Mar 15, 2013 of Apr. 12, 2011. Suen, Shing-Yi, et al. “Comparison of Ligand Density and Protein (65) Prior Publication Data Adsorption on Dye Affinity Membranes Using Difference Spacer Arms'. Separation Science and Technology, 35:1 (2000), pp. 69-87. US 2013/0210111A1 Aug. 15, 2013 Related U.S. Application Data Primary Examiner — Chester Barry (62) Division of application No. 13/580,055, filed as (74) Attorney, Agent, or Firm — Cantor Colburn LLP application No. -
The Role of Elastases in Pancreatic Diseases
The role of elastases in pancreatic diseases Ph.D. Thesis Anna Zsófia Tóth M.D. Supervisors: Prof. Péter Hegyi, M.D., Ph D., D.Sc. Prof. Miklós Sahin-Tóth, M.D., Ph.D., D.Sc Doctoral School of Theoretical Medicine Szeged 2018. Content Content ....................................................................................................................................... 1 1. List of abbreviations ........................................................................................................... 3 2. Introduction ......................................................................................................................... 5 2.1. Exocrin pancreatic insufficiency ................................................................................. 5 2.2. Pancreatic elastases ...................................................................................................... 5 2.3. Pancreatic function tests based on detection of elastases ............................................ 7 2.4. Possible role of elastases in chronic pancreatitis ......................................................... 8 3. Aims .................................................................................................................................. 10 3.1. ScheBo Pancreatic Elastase 1 Test Study .................................................................. 10 3.2. Genetic analysis study ............................................................................................... 10 4. Materials and methods ..................................................................................................... -
Evaluation of the Efficacy of Three Plant Extracts Against Venoms of Five Snake Species in Northern Nigeria
EVALUATION OF THE EFFICACY OF THREE PLANT EXTRACTS AGAINST VENOMS OF FIVE SNAKE SPECIES IN NORTHERN NIGERIA BY YUNUSA YAHAYA DEPARTMENT OF VETERINARY PUBLIC HEALTH AND PREVENTIVE MEDICINE FACULTY OF VETERINARY MEDICINE AHMADU BELLO UNIVERSITY, ZARIA MARCH 2017 EVALUATION OF THE EFFICACY OF THREE PLANT EXTRACTS AGAINST VENOMS OF FIVE SNAKE SPECIES IN NORTHERN NIGERIA BY YUNUSA Yahaya P15VTPH9009 BSc, (1997), MSc (2006) (ABU, ZARIA) A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA IN PARTIAL FULFILMENT OF THE REQUIREMENTS FOR THE AWARD OF DEGREE OF DOCTOR OF PHILOSOPHY IN VETERINARY PUBLIC HEALTH AND PREVENTIVE MEDICINE DEPARTMENT OF VETERINARY PUBLIC HEALTH AND PREVENTIVE MEDICINE, AHMADU BELLO UNIVERSITY, ZARIA March, 2017 i DECLARATION I declare that the work in this thesis entitled “Evaluation of the efficacy of three plant extracts against venoms of five snake species in Northern Nigeria” has been performed by me in the Department of Veterinary Public Health and Preventive Medicine. The information derived from the literature has been duly acknowledged in the text and list of references provided. No part of this dissertation was previously presented for another degree or diploma at this or any other Institution. _____________________ ___________________ ______________ YUNUSA Yahaya Signature Date ii CERTIFICATION The thesis entitled “Evaluation of the efficacy of three plant extracts against venoms of five snake species in Northern Nigeria” by YUNUSA Yahaya meets the regulations governing the award of Doctor of philosophy degree in Veterinary Public Health and Preventive Medicine of Ahmadu Bello University, Zaria and is approved for its contribution to knowledge and literary presentation. -
Isolation and Characterization of Snake Venom Proteins and Peptides from Members of Viperidae and Elapidae Snake Families from Kilifi County
ISOLATION AND CHARACTERIZATION OF SNAKE VENOM PROTEINS AND PEPTIDES FROM MEMBERS OF VIPERIDAE AND ELAPIDAE SNAKE FAMILIES FROM KILIFI COUNTY OTIENO KEPHER ONYANGO MASTER OF SCIENCE (Molecular Medicine) JOMO KENYATTA UNIVERSITY OF AGRICULTURE AND TECHNOLOGY 2018 Isolation and Characterization of Snake Venom Proteins and Peptides from Members of Viperidae and Elapidae Snake Families from Kilifi County Otieno Kepher Onyango A thesis submitted in partial fulfillment for the Degree of Master of Science in Molecular Medicine in the Jomo Kenyatta University of Agriculture and Technology 2018 DECLARATION This thesis is my original work and has not been presented for a degree in any other University. Signature: ……………………… Date: ……………………….. Otieno Kepher Onyango This thesis has been submitted for examination with our approval as University supervisors. Signature: ……………………..... Date: …………………………. Prof. Joseph Kangangi Gikunju, PhD JKUAT, Kenya Signature: ……………………….... Date: ………………………… Dr. Kimani Gachuhi, PhD KEMRI, Kenya. ii DEDICATION This work is dedicated to my parents, Mr.Gabriel Otieno and Mrs. Christine Otieno, my wife Mrs. Florence Onyango and our two daughters M/s. Michelle and M/s. Maureen for making sure that I had easy moment during my studies. I wish you many blessings from God Almighty. iii ACKNOWLEDGEMENT Completing a major research project in snake venom proteomics would not have been made possible without the moral and material support from several individuals who deserve so much appreciation more than just a piece of an acknowledgement. First to my hardworking university and research supervisors: Prof. Joseph K.Gikunju and Dr. Kimani Gachuhi, I want to thank you so much for providing me with the required academic and research advice for the successful completion of my research work and their assistance with securing laboratory space within the required time.