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(12) Patent Application Publication (10) Pub. No.: US 2010/0166677 A1 Ptchelintsev (43) Pub

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(12) Patent Application Publication (10) Pub. No.: US 2010/0166677 A1 Ptchelintsev (43) Pub US 201001 66677A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2010/0166677 A1 Ptchelintsev (43) Pub. Date: Jul. 1, 2010 (54) USE OF TILIACORATRIANDRA IN Publication Classification COSMETCS AND COMPOSITIONS (51) Int. Cl. THEREOF A636/00 (2006.01) A6IR 9/12 (2006.01) (75) Inventor: Dmitri S. Pitchelintsev, Jersey City, A61O 19/00 (2006.01) NJ (US) (52) U.S. Cl. ........................................... 424/47; 424/725 (57) ABSTRACT Correspondence Address: The present disclosure relates to compositions and methods AVON PRODUCTS, INC. for treating, preventing and improving the condition and aes AVON PLACE thetic appearance of skin, particularly, treating, preventing, SUFFERN, NY 10901 (US) ameliorating, reducing and/or eliminating fine lines and/or wrinkles of skin, where the compositions include natural (73) Assignee: Avon Products, Inc., Suffern, NY plant constituents which increase expression levels genes, (US) collagen replacement and retention, and cell proliferation of epidermis and dermis associated with the dermatological (21) Appl. No.: 12/345,707 signs of aging. The compositions of the invention are topi cally applied to the skin, or are delivered by directed means to a site in need thereof, once daily in an amount effective in (22) Filed: Dec. 30, 2008 improving the condition and aesthetic appearance of skin. Patent Application Publication Jul. 1, 2010 Sheet 1 of 4 US 2010/0166677 A1 175 S 150 E 5C 125 Š 100 S O O ------------ - - - - - - - - - - - - ---- - - - - - - r--- - - --- -- - - - - - - - - - - O 50 100 150 200 Tiliacora triandra extract (ug/ml) F.G. 1 Patent Application Publication Jul. 1, 2010 Sheet 2 of 4 US 2010/0166677 A1 125 - 3 100 75 a 50 O as 0 ------ --- -r- O SO 00 150 200 Tiliacora triandra extract (ug/ml) F.G. 2 Patent Application Publication Jul. 1, 2010 Sheet 3 of 4 US 2010/0166677 A1 A Lane 1 2 O 3 4 5 B 2O OO O 85 60 s 48 S'we' 4. C 9 20 mM O O 50 O) phenanthroline Tiliacora triandra extract (ug/ml) FG, 3 Patent Application Publication Jul. 1, 2010 Sheet 4 of 4 US 2010/0166677 A1 A. 125 - 95 - o 100 | 86 80 e is e s 50 C. SS N O 25 O |- ------- --- ------------ - - - O O 50 100 Tiliacora triandra extract (ug/ml ) B. OO 80 30 --------- --------- --- --------- - -- aw O SO 100 200 Tiliacora triandra extract (ug/ml ) F.G. 4 US 2010/01 66677 A1 Jul. 1, 2010 USE OF TLACORATRANDRAIN dermatological signs of aging, including environmental COSMETCS AND COMPOSITIONS stress, due to a progressive degradation of the epidermal and THEREOF dermal skin layers, would be advantageous for the formula tion of treatments and products for the skin. As described FIELD herein, novel and beneficial methods and compositions, as well as their mode of action, for the treatment of wrinkles and 0001. This disclosure generally relates to cosmetic com the like, as well as for personal care products for the skin, are positions and their use, and more particularly to cosmetic provided herein. compositions and to their use in improving the condition and appearance of skin. SUMMARY BACKGROUND 0007. It is an object of the present disclosure to provide a topical composition having a natural plant material, blends 0002 There is an increasing demand in the cosmetics thereof, or components therefrom, in an amount Sufficient to industry to develop products that may be applied topically to prevent, ameliorate and/or reduce dermatological signs of the skin that improve the condition and appearance of skin. chronologically or hormonally-aged orphoto-aged skin, Such Consumers are interested in mitigating or delaying the der as fine lines, wrinkles, sagging skin, and other conditions due matological signs of chronologically- or hormonally-aged to a progressive degradation of the skin cell growth, prolif skin, as well as skin aging due to the environmental stress, eration and functionality in the epidermal and dermal layer, Such as fine lines, wrinkles, sagging skin and other conditions where the plant material, blends thereofor components there due to a progressive loss of cell growth, proliferation and from are in a cosmetically, dermatologically acceptable functionality in the epidermal and dermal skin layers. During vehicle, carrier, or diluent. the aging process, the complexion of the skin, i.e., the color 0008. It is another object of the present disclosure to pro and appearance of the skin, deteriorates slowly from aging vide topical compositions having an extract from at least and/or exposure to environmental stress, e.g., Sunlight. Tiliacora triandra Diels plant, in an amount effective to treat, Numerous cosmetic and medical treatments have been devel prevent, control, ameliorate, inhibit, and/or reduce dermato oped in an attempt to treat environmentally damaged, aging or logical signs of chronologically or hormonally-aged or aged skin. However, such cosmetics or treatments commonly photo-aged skin, such as fine lines, wrinkles, sagging skin, contain organic acids as their active ingredients or compo and other conditions due to a progressive degradation of the nents, and are frequently associated with consumer discom skin cell growth, proliferation and functionality in the epider fort, Such as burning, itching, and redness. mal and dermal layer. 0003. Therefore, there remains a general need in the cos 0009. Yet another object of the disclosure to provide a metics industry for products that retard or counter the aging topical composition having a natural plant ingredient, which effects on the skin, and more specifically for products that enhances dermal-epidermal cell growth, proliferation and produce such effects without undesirable side effects. In par functionality of skin and mechanical properties of skin, as ticular, there remains a need for topically applied cosmetic well as, cell-cell cohesion in the epidermis of skin, anchoring compositions that have anti-aging and skin texture benefits of the cells of skin, tissue stability of skin, and communica using natural plant materials as active components. tion between cells of skin, in order to improve the aesthetic 0004 Active ingredients or components derived from appearance of skin. plants and plant seeds have commonly been employed for a 0010. It is still a further object of the present disclosure to myriad of medicinal, therapeutic and cosmetic purposes. provide a method of improving the appearance of skin, Such actives may be obtained from the entire plant or various including treating the effects of aging in the skin, by topically parts of a plant, Such as seeds, needles, leaves, roots, bark, applying the compositions of the disclosure to the skin. cones, stems, rhizomes, callus cells, protoplasts, organs and 0011 Further objects, features and advantages of the organ systems, and meristems. Active ingredients or compo present disclosure will be better appreciated upon a reading of nents are incorporated in compositions in a variety of forms. the detailed description. Such forms include a pure or semi-pure component, a Solid or 0012. These and other objects and advantages of the liquid extract or derivative, or a solid natural plant material. present disclosure, and equivalents thereof, are achieved by Plant material may be incorporated in a variety of subforms cosmetic compositions having a single natural botanical Such as whole, minced, ground or crushed, or otherwise ingredient or blends of natural botanical ingredients, or com physically modified for incorporation into a composition. ponents therefrom, and use of Such compositions for topical 0005 Tiliacora triandra Diels of the Tiliacora family, also application. known as Yanang, is a species of flowering plant native to mainland Southeast Asia and used particularly in the cuisines BRIEF DESCRIPTION OF THE FIGURES of northeast Thailand and Laos. It is a climbing plant with mostly single, Smooth, oval-shaped, deep green leaves and 0013 FIG. 1 shows the effect of Tiliacora triandra extract yellowish flowers. In traditional Southeast Asian medicine, on fibroblast cell proliferation/survival. The survival of Tiliacora triandra has been used as an herbal medicine for untreated fibroblast cells (Oug/ml of extract) was assigned the fever relief, alcohol intoxication, inflammation, and bacterial/ value of 100%. The survival of treated fibroblast cells at 25, fungal infection. For instance, the use of Tiliacora triandra 50, 75, 100, 150, and 200 mg/ml of the extract was deter Diels against plasmodium falciparum (cause malaria in mined by comparing the number of treated cells in reference humans) is disclosed in Pavanand et al., Phytother: Res., 3, to the untreated cells (control). The standard deviation of each 215-217 (1989). extract concentration is represented by error bars. 0006 Safe, effective and new components of composi 0014 FIG. 2 shows the effect of Tiliacora triandra extract tions to treat, prevent, reduce, inhibit and/or improve the on keratinocyte cell proliferation/survival. The survival of US 2010/01 66677 A1 Jul. 1, 2010 untreated keratinocyte cells (Oug/ml of extract) was assigned (UV) Sunlight. As commonly known, the skin is composed of the value of 100%. The survival of treated keratinocyte cells multiple layers of cells, which may be broadly divided into at 25, 50, 75, 100, 150, and 200 mg/ml of the extract was two sections—the top epidermis and the underlying dermis determined by comparing the number of treated cells in ref layers. erence to the untreated cells (control). The standard deviation of each extract concentration is represented by error bars. Epidermis 0015 FIG.3 shows the effect of Tiliacora triandra extract 0021. The epidermis is the topmost layer of the skin that on gelatinolytic activity. FIG. 3A shows lanes 1-5 of the provides waterproofing and serves as a barrier to infection gelatin Zymogram: lane 1 is a positive control (10 mM and other external elements. This layer is mostly consists of Phenanthroline); lane 2 is a negative control (untreated); and keratinocyte cells, which originate in the basal layer (the lanes 3-5 are samples treated with 10, 50, and 100 g/ml of the deepest layer of the epidermis) from the division of kerati extract.
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