New Tools for Studying Microglia in the Mouse and Human CNS
New tools for studying microglia in the mouse and human CNS Mariko L. Bennetta,1,F.ChrisBennetta,b, Shane A. Liddelowa,c, Bahareh Ajamid, Jennifer L. Zamaniana, Nathaniel B. Fernhoffe,f,g,h, Sara B. Mulinyawea, Christopher J. Bohlena, Aykezar Adila, Andrew Tuckera, Irving L. Weissmane,f,g,h, Edward F. Changi, Gordon Lij, Gerald A. Grantj, Melanie G. Hayden Gephartj, and Ben A. Barresa,1 aDepartment of Neurobiology, Stanford University School of Medicine, Stanford, CA 94305; bDepartment of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Stanford, CA 94305; cDepartment of Pharmacology and Therapeutics, University of Melbourne, Melbourne, VIC, Australia, 3010; dDepartment of Neurology, Stanford University School of Medicine, Stanford, CA 94305; eInstitute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA 94305; fLudwig Center for Cancer Stem Cell Research and Medicine, Stanford University School of Medicine, Stanford, CA 94305; gStanford Cancer Institute, Stanford University School of Medicine, Stanford, CA 94305; hDepartment of Pathology, Stanford University School of Medicine, Stanford, CA 94305; iUniversity of California, San Francisco Epilepsy Center, University of California, San Francisco, CA 94143; and jDepartment of Neurosurgery, Stanford University School of Medicine, Stanford, CA 94305 Contributed by Ben A. Barres, January 12, 2016 (sent for review November 8, 2015; reviewed by Roman J. Giger and Beth Stevens) The specific function of microglia, the tissue resident macrophages and well-validated antibodies to known antigens yet exist to of the brain and spinal cord, has been difficult to ascertain because specifically and stably identify microglia. of a lack of tools to distinguish microglia from other immune cells, Therefore, we sought a molecular marker that would allow for thereby limiting specific immunostaining, purification, and manipu- the identification, isolation, and study of microglia across many lation.
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