10.1071/IS14023_AC © CSIRO 2014 Supplementary Material: Invertebrate Systematics 28 , 555–563.

SUPPLEMENTARY MATERIAL

Genetic data confirm the species status of Sepsis nigripes Meigen (Diptera : Sepsidae) and adds one species to the Alpine fauna while questioning the synonymy of Sepsis helvetica Munari

Patrick T. Rohner A,E , Yuchen Ang B, Zhao Lei B, Nalini Puniamoorthy C, Wolf U. Blanckenhorn A and Rudolf Meier B,D

AInstitute of Evolutionary Biology and Environmental Studies, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.

BDepartment of Biological Sciences, National University of Singapore, 14 Science Dr 4, Singapore 117543, Singapore.

CDepartment of Biology, Life Sciences Complex, Syracuse University, 107 College Place, Syracuse, NY 13244, USA.

DUniversity Scholars Programme, National University of Singapore, Singapore 138593, Singapore.

ECorresponding author. Email: [email protected]

Page 1 of 1 Dicranosepsis distincta Outgroups: Allosepsis sp.1 Sepsis arotrolabis 100 Sepsis thoracica (Europe) Sepsis thoracica (South Africa) 98.2 47.1 Sepsis neglecta 91.6 100 Sepsis cynipsea 100 Sepsis neocynipsea 100 Sepsis latiforceps 100 Sepsis punctum 88.4 43.7 100 Sepsis luteipes (Europe) Sepsis luteipes (North America) 99 Sepsis violacea 97.2 100 Sepsis fulgens Sepsis orthocnemis Sepsis fissa 97.8 56.9 Sepsis flavimana Sepsis nigripes 100 98.2 Sepsis pyrrhosoma 64.7 Sepsis biflexuosa 98.6 100 Sepsis duplicata Sepsis secunda 60.5 Sepsis dissimilis Sepsis hirsuta Sepsis frontalis 67.9 100 100 Sepsis niveipennis (African) Sepsis niveipennis (Asian) 48.7 58.7 Sepsis nitens Sepsis sp. (Isangi) 53.7 Sepsis sepsi 100 100 Sepsis coprophila Sepsis lateralis 1.0

Supplementary Fig. 1. MP bootstrap tree (indel = ?) Dicranosepsis distincta Outgroups: Allosepsis sp.1 Sepsis thoracica (Europe) 100 Sepsis thoracica (South Africa) 100 Sepsis neglecta 80.8 Sepsis neocynipsea 100 100 Sepsis cynipsea Sepsis latiforceps 100 100 Sepsis punctum 96 Sepsis luteipes (Europe) 100 Sepsis luteipes (North America) 98.4 48.8 Sepsis violacea 95.2 Sepsis fulgens 100Sepsis orthocnemis Sepsis fissa Sepsis flavimana 93.6 59.2 Sepsis nigripes 98.8 100 Sepsis pyrrhosoma 54.8 Sepsis biflexuosa 99.2 Sepsis duplicata 100 Sepsis secunda Sepsis arotrolabis Sepsis dissimilis 81.2 46.8 Sepsis hirsuta Sepsis frontalis 81.2 100 Sepsis niveipennis (African) 100 Sepsis niveipennis (Asian) 91.6 Sepsis nitens 67.6 Sepsis sp. (Isangi) 88 Sepsis sepsi 100 Sepsis coprophila 100 Sepsis lateralis 0.02

Supplementary Fig. 2. ML bootstrap tree under one partition for all data Dicranosepsis distincta Outgroups: Allosepsis sp.1 Sepsis arotrolabis Sepsis thoracica (Europe) 100 56.4 Sepsis thoracica (South Africa) 44.8 98 Sepsis neglecta Sepsis cynipsea 100 Sepsis neocynipsea 100 100 Sepsis latiforceps 100 Sepsis punctum 94.4 Sepsis luteipes (North America) 40 100 Sepsis luteipes (Europe) 97.2 Sepsis violacea 93.2 Sepsis fulgens 100Sepsis orthocnemis Sepsis fissa Sepsis flavimana 98.8 76.4 100 Sepsis nigripes 98.4 Sepsis pyrrhosoma 69.6 Sepsis biflexuosa 91.2 100 Sepsis duplicata Sepsis secunda Sepsis dissimilis 88 Sepsis hirsuta 88.8 Sepsis frontalis 100 100 Sepsis niveipennis (African) Sepsis niveipennis (Asian) 90.4 Sepsis nitens 60 Sepsis sp. (Isangi) 90 Sepsis sepsi 100 Sepsis coprophila 100 Sepsis lateralis 0.03

Supplementary Fig. 3. ML bootstrap tree partitioned by codon position