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PARL Antibody Cat PARL Antibody Cat. No.: 4761 PARL Antibody Immunocytochemistry of PARL in 3T3 cells with PARL Immunofluorescence of PARL in 3T3 cells with PARL antibody at 2.5 μg/mL. antibody at 2.50 μg/mL. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse, Rat HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%) PARL antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human PARL. IMMUNOGEN: The immunogen is located within amino acids 40 - 90 of PARL. TESTED APPLICATIONS: ELISA, ICC, IF, WB September 25, 2021 1 https://www.prosci-inc.com/parl-antibody-4761.html PARL antibody can be used for detection of PARL by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight of ~42kDa, PARL is observed at a higher molecular weight in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. APPLICATIONS: Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. POSITIVE CONTROL: 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate 2) Cat. No. 17-201 - 3T3/BALB Cell Slide Properties PURIFICATION: PARL Antibody is affinity chromatography purified via peptide column. CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: PARL Antibody is supplied in PBS containing 0.02% sodium azide. CONCENTRATION: 1 mg/mL PARL antibody can be stored at 4˚C for three months and -20˚C, stable for up to one STORAGE CONDITIONS: year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: PARL PARL Antibody: PSARL, PSARL1, RHBDS1, PRO2207, PSENIP2, PSARL, Presenilins- ALTERNATE NAMES: associated rhomboid-like protein, mitochondrial, Mitochondrial intramembrane cleaving protease PARL, Pbeta ACCESSION NO.: Q9H300 PROTEIN GI NO.: 143811433 GENE ID: 55486 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References September 25, 2021 2 https://www.prosci-inc.com/parl-antibody-4761.html PARL Antibody: PARL is a mitochondrial integral membrane protein and a member of the highly conserved rhomboid superfamily of membrane proteins. PARL is required for the control of apoptosis during postnatal growth and is required for the processing of OPA1, a protein that prevents the release of cytochrome C from the mitochondria in response to apoptotic signals. In lymphocytes and neurons, PARL's association with other proteins, BACKGROUND: such as the Bcl-2 family-related protein Hax1 and Omi can lead to the proteolytic processing of Omi by PARL, preventing the accumulation of Bax, thereby suppressing apoptosis. Variations in this gene have been associated with increased risk for type 2 diabetes. PARL may also play a role in the progression of Alzheimer's disease through its associations with presenilin-1 and -2. Multiple isoforms of PARL are known to exist. 1) Pellegrini L, Passer BJ, Canelles M, et al. PAMP and PARL, two novel putative REFERENCES: metalloproteases interacting with the COOH-terminus of Presenilin-1 and -2. J. Alzheimers Dis.2001; 3:181-90. 2) Cipolat S, Rudka T, Hartmann D, et al. Mitochondrial rhomboid PARL regulates cytochrome c release during apoptosis via OPA1-dependent cristae remodeling. Cell2006; 126:163-75. 3) Chao JR, Parganas E, Boyd K, et al. Hax1-mediated processing of HtrA2 by PARL allows survival of lymphocytes and neurons. Nature2008; 452:98-102. 4) Walder K, Kerr-Bayles L, Civitarese A, et al. The mitochondrial rhomboid protease PSARL is a new candidate gene for type 2 diabetes. Diabetologia2005; 48:459-68. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 25, 2021 3 https://www.prosci-inc.com/parl-antibody-4761.html.
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