Research in Biotechnology 2020

Research in Iowa State University Office of Biotechnology Biotechnology 1210 Molecular Biology Building 2437 Pammel Drive Ames, IA 50011-1079 2020 tel: 515-294-9818 e-mail: [email protected] www.biotech.iastate.edu

A publication of the Office of Biotechnology and the Office of the Vice President for Research Iowa State University Research in Biotechnology 2020

This research directory serves as a resource for faculty at Iowa State University who are interested in developing interdisciplinary projects and for individuals in other universities, government and industry who are interested in establishing collaborative research relationships with the university.

For more information on the topics covered in this publication, please contact the Iowa State University Office of Biotechnology, 1210 Molecular Biology Building, 2437 Pammel Drive, Ames, Iowa 50011-1079. Telephone: 515-294-9818 E-mail: [email protected] Web Address: www.biotech.iastate.edu

Iowa State University does not discriminate on the basis of race, color, age, ethnicity, religion, national origin, pregnancy, sexual orientation, gender identity, genetic information, sex, marital status, disability, or status as a U.S. Veteran. Inquiries regarding non-discrimination policies may be directed to Office of Equal Opportunity, 3410 Beardshear Hall, 515 Morrill Road, Ames, Iowa 50011, Tel. 515 294-7612, Hotline 515-294-1222, email [email protected]

Research in Biotechnology 2020 Table of Contents

ISU Office of Biotechnology Overview ...... 1 Faculty Profiles...... 6 Services and Facilities...... 61 Technology Transfer, Patents and Licensing...... 61 Animal Gene Transfer Facility ...... 61

BioCentury Research Farm...... 61

Biomolecular Nuclear Magnetic Resonance Facility...... 62

Chemical Instrumentation Facility...... 62 Comparative Pathology Core Service...... 63 Crops Utilization Research (Center for)...... 63 DNA Facility ...... 63 Doubled Haploid Facility ...... 64

Fermentation Facility ...... 65 Flow Cytometry Facility ...... 65 Genome Informatics Facility...... 66

Genomic Technologies Facility...... 66 Grain Quality Laboratory...... 67 High Resolution Microscopy Facility (Roy J. Carver)...... 67 Hybridoma Services...... 69 Macromolecular X-ray Crystallography Facility...... 69 Materials Analysis and Research Laboratory...... 70 Materials Preparation Center...... 71 Metabolomics Research Laboratory (W. M. Keck)...... 71

Microfabrication Facility (W. M. Keck)...... 71 Nutrition and Wellness Research Center (Food Science and Human Nutrition)...... 71 Olfactometry and Air Quality Laboratories ...... 72 Plant Transformation Facility ...... 72 Protein Facility ...... 73 Faculty Indexed by Department ...... 75

Biotechnology Overview Iowa State University An Overview of Biotechnology Research Biotechnology 2020 at Iowa State University

Built on a Strong Foundation • 2003 – The BOEC teaches its 1,000th teacher. The bio- Iowa State University is a land-grant institution that has ethics outreach coordinator taught Iowa State’s first-ever served society in three centuries. The State of Iowa con- summer bioethics workshop to help teachers and exten- tinues to provide financial support to maintain Iowa State sion professionals incorporate ethics into life sciences University’s leadership in biotechnology education, research classrooms and discussions. Public forums were held to and outreach. In 2020, Iowa State will continue to empha- discuss USDA regulations on field testing pharmaceutical size innovative education for young people and adults, de- crops. velopment of new products and processes from basic and • 2004 – The biotechnology program celebrates its 20th applied research and service to the biotechnology industry. year. A record number of companies and organizations Highlights of Iowa State’s program include: participate in Biotechnology Career Day. The BOEC reaches its 20,000th user. The bioethics outreach program adds two • 1984 – A Biotechnology Council made up of faculty new online courses for educators. members from five academic colleges is established. • 2005 – The number of new faculty in molecular biology Today, the Council continues to make recommendations to with start-up support from the Office of Biotechnology the Vice President for Research for biotechnology educa- nears 110. The Office awards its 187th graduate fellow- tion, research and outreach. ship. A 228-page curriculum for grades 9-12, From Mendel • 1986 – Iowa provides $17 million over a four-year period to Markers, is released. The Office grants its 65th faculty to establish Iowa State’s biotechnology program. development award since 1992. The BOEC celebrates its • 1988 – The Iowa State Public Education Program in fifth year. Biotechnology begins. • 2006 – The BOEC is utilized by 1,634 people. Free • 1989 – Iowa State conducts the first field test in the supplies for laboratory experiments in biotechnology are nation of a genetically engineered tree, a pest-resistant provided to 129 schools in Iowa for the benefit of 14,167 poplar variety. students. Over 1,700 individuals participate in off-campus • 1990 – Iowa State hosts a national meeting to assess the BOEC activities. The number of new faculty in molecular benefits and risks of introducing herbicide tolerant crops in biology with start-up support from the Office of Iowa. Biotechnology reaches 116. The W. M. Keck Laboratory • 1991 – Two Iowa State biotechnology researchers win for High Throughput Atom-Scale Analysis is initiated. The one of the coveted R&D 100 Awards presented by R&D Nuclear Magnetic Resonance (NMR) Facility installs a new Magazine to the top 100 product innovations of the year. 700 MHz spectrometer. • 1992 – The Molecular Biology Building, built at a cost of • 2007 – The Office of Biotechnology awards its 199th $30.5 million, is dedicated. graduate fellowship. The number of new faculty in molecu- • 1995 – Thirty-one Iowa counties hold biotechnology lar biology with start-up support from the Office reaches camps for youth. 121. The Office directly supports five faculty-organized • 1996 – Iowa State hosts an international conference on biotechnology symposia. The Microscopy and NanoImag- extension’s role in biotechnology education. ing Facility installs a new scanning transmission electron • 1999 – Iowa State’s Biotechnology Outreach Education microscope (STEM). The Macromolecular X-ray Crystallog- Center (BOEC) is built. First Biotechnology Career Day at- raphy Facility opens. The BOEC is utilized by 3,690 individu- tracts more than 400 students. als. Free supplies are provided for laboratory experiments • 2000 – The Office of Biotechnology awards its 100th in biotechnology to 115 schools in Iowa for over 14,000 biotechnology graduate fellowship. The international confer- students. The number of biotechnology-related research ence, Engaged Institutions’ Role in Biotechnology Educa- projects funded through the Grow Iowa Values Fund pro- tion, attracts more than 225 educators. gram reaches 28 in just three years. The Bioethics Program • 2001 – A curriculum about a new soybean with improved sponsors a lecture series covering ethical and legal issues digestibility is published for students in grades 9 to 12. A related to ethics in biotechnology. regional meeting explores strategies for the coexistence • 2008 – Graduate fellowships from the Office of Biotech- of genetically modified (GM), non-GM and organic crop nology top 200. A Carver Trust grant supports the purchase production. of a new confocal microscope. A DNA sequencing instru- • 2002 – A full-time bioethics outreach coordinator is hired mentation shared-use agreement is developed with the to work with Iowa State Extension. A free CD that provides University of Iowa. The renovation of the Microscopy and laboratory exercises, PowerPoint presentations and other NanoImaging Facility is completed. The Cell Facility is tools for life sciences teachers is made available to Iowa’s re-christened Flow Cytometry Facility. The Office of K-12 educators. Service Facilities publication begins. Biotechnology website is re-designed. More than 16,000

1 Research in Biotechnology 2020 students in more than 150 Iowa schools receive free Facility joins the Office of Biotechnology core instrumenta- supplies for biotechnology experiments, bringing the total tion facilities. The Genome Informatics Facility moves to number of students who have experienced biotechnology Science I. activities in their classrooms using the free supplies and • 2013 – The Iowa State Biotechnology Public Education equipment to over 200,000. Program marks 20 years of providing free equipment, • 2009 – 25th anniversary of the Office of Biotechnology. supplies and instruction, to the benefit of 289,410 K-16 Walter Fehr writes and releases the book, The First 25 Iowa students during that time. In 2013 alone, the Bio- Years of the Office of Biotechnology. James Reecy be- technology Outreach Education Center (BOEC) provides comes the new director of the Office of Biotechnology. supplies for biotechnology experiments to 113 Iowa Nearly 12,000 Iowa high school students in 116 schools schools, serving 11,621 students, and directly serves 3,209 receive supplies for biotechnology experiments. A new students through Mike Zeller, BOEC Outreach Education Next Gen Sequencer is installed in the DNA Facility. The Coordinator, either on the Iowa State campus or at schools Confocal Microscopy Facility installs a new state-of-the-art throughout the state. The Office of Biotechnology website microscope. is redesigned. Graduate fellowship awards to date number • 2010 – The Office of Biotechnology begins offering re- 237, while Office-supported faculty development activities sources to assist faculty or professional and scientific staff number 105. Joel Nott, Assistant Scientist III and manager in coordination, budget development, and editing of grant of the Protein Facility, is awarded a Professional and Scien- proposals or subcontracts related to biotechnology or in- tific Excellence Award, recognizing and honoring those who strumentation acquisitions. The BOEC begins working with have achieved excellence in their respective fields. The Iowa State’s Bioeconomy Institute (BEI) and the Center for DNA Facility acquires a Fluidigm® BioMark™ HD system, Biorenewable Chemicals (CBiRC) to develop K-12 curricu- which uses microfluidic technology to accurately combine lum modules on biorenewables. The Materials Analysis and reagents and samples to perform thousands of individual Research Laboratory, Proteomics Facility and Macromo- nanoliter-scale PCR or genotyping assays in a single run. lecular X-Ray Crystallography Facility become part of the The Materials Analysis and Research Laboratory upgrades core instrumentation facilities administered by the Office thermal gravimetric analysis and x-ray fluorescence sample of Biotechnology. The Proteomics Facility and the Protein preparation equipment and installs a heating stage option Facility combine their services. The Flow Cytometry Facility for the scanning electron microscope, which performs installs new cell sorting instrumentation. Biotechnology dynamic in situ analysis of samples in a temperature range experiment supplies are provided to 121 schools serving from ambient to 1,000°C. over 10,000 students. Over 2,200 students and teachers • 2014 – The Office of Biotechnology supports 105 faculty have educational experiences through the Biotechnology development activities in its 30-year history. The Protein Outreach Education Center. Facility adds a new Typhoon scanner for imaging proteins • 2011 – The Office hires a full-time manager for the and a Q Exactive Hybrid™ Quadrupole-Orbitrap Mass Macromolecular X-ray Crystallography Facility, James Spectrometer. The Macromolecular X-ray Crystallography Phillips. The Genome Informatics Facility comes online with Facility acquires a TTP Labtech Mosquito® Crystal liquid Andrew Severin, manager. The hard-copy Biotechnology handler which allows faster and more cost-effective protein Update morphs into the electronic Biotechnology E-News. crystallization screening. The Chemical Instrumentation The Office of Biotechnology assists the Office of the Vice Facility upgrades to an LCMS 2020 and adds a TGA/DSC. President for Research in starting the E-News for Research- The Genome Informatics Facility aquires an archive server ers. The DNA facility’s new HiSeq and cBot equipment with 132 Terabytes of backup storage. The W.M. Keck comes online, creating a 10-fold increase in DNA sequenc- Metabolomics Research Laboratory upgrades its newest ing capacity on campus. The BOEC provides supplies for high resolution mass spectrometer, FT-ICR, for image-mass biotechnology experiments to 123 Iowa schools, serving spectrometry. The Biotechnology Public Education Program 13,225 students. Students directly served by Mike Zeller, marks 21 years of providing free equipment, supplies and BOEC Outreach Education Coordinator, in the BOEC and instruction to the benefit of 302,390 K-16 Iowa students traveling to schools totaled 2,612 for the year. In coordina- during that time. The Biotechnology Outreach Education tion with the College of Engineering, the Office is granted a Center (BOEC) provides supplies for biotechnology experi- Roy J. Carver Charitable Trust award to upgrade microsco- ments to 114 Iowa schools, serving 10,002 students, and py on campus. Graduate fellowship awards to date number directly serves 3,141 students through Mike Zeller, BOEC 217, while the Office supports 95 faculty development Outreach Education Coordinator, either on the Iowa State activities. campus or at schools throughout the state. • 2012 – The Iowa State Biotechnology Outreach Education • 2015 – The Biotechnology Outreach Education Center Center and Mike Zeller receive Friend of Science Awards (BOEC) provides supplies for biotechnology experiments from the Iowa Academy of Science for contributions to sci- to 118 Iowa schools, serving 11,960 students, and directly ence education. The BOEC provides supplies for biotech- serving 3,475 students through Mike Zeller, BOEC Out- nology experiments to 121 Iowa schools, serving 13,042 reach Education Coordinator, either on the Iowa State cam- students. Students directly served by the BOEC or traveling pus or at schools throughout the state. Office of Biotech- to schools totaled 3,651 for the year. Graduate fellowship nology Director Dr. James Reecy is named as Fellow of the awards to date number 229, while the Office supports 101 American Association for the Advancement of Science. The faculty development activities. The Plant Transformation DNA Facility installs a Pacific Biosciences RS II sequencer 2 Biotechnology Overview capable of providing long-read sequences averaging 15,000 Synergy LX Multimode Plate Reader. The DNA Facility also base pairs per run. The Materials Analysis Research Labora- expands services and reduces costs of library and sample tory acquires an X-ray photoelectron spectrometer for char- prep for researchers by changing in sourcing. The Genome acterization of the chemical states of the surfaces of solids. Informatics Facility creates an online Bioinformatics Work- The Flow Cytometry Facility purchases a new 14-color flow book that enables researchers to design and analyze high- cytometer with a grant from the Roy J. Carver Charitable throughput sequencing data. Trust. • 2019 – Hybridoma Services becomes part of the Protein • 2016 – The Roy J. Carver Charitable Trust of Muscatine, Facility. The Flow Cytometry Facility purchases a Beckman Iowa, committed $448,659 to Iowa State University to Coulter MoFlow XDP. Able to sort individual chromosomes, purchase microscopy equipment for the Office of Biotech- the MoFlow XDP brings a completely new technological nology. The Microscopy and NanoImaging Facility (MNIF) capability to Iowa State. The Research Experiences for in Bessey Hall was gifted approximately $2,500,000 in mi- Teachers Program moves to the Office of Biotechnology. croscopic and ancillary instrumentation by DuPont Pioneer The Biotechnology Outreach Education Center (BOEC) from its Wilmington, Delaware, Imaging Facility. The Carver provides supplies for biotechnology experiments to 74 Iowa grant and the DuPont gift were combined with existing schools, serving 8,259 students, and directly serves 1,400 microscopy facilites on campus to create the Roy J. Carver students through student visits to the Iowa State campus High Resolution Microscopy Facility. The DNA Facility or by BOEC visits to schools throughout the state. upgraded its short-read sequencing capability by installing an Illumina HiSeq3000 that generates twice the data in half Innovative Research the time. The Protein Facility acquired the Proteome Dis- For Iowa State’s biotechnology researchers, excellence is a coverer 2.1 which has the capability to quantify identified way of life. Iowa State has more than 350 biotechnologists proteins using isotope labeling (SILAC), isobaric mass tags from 34 departments in five colleges. Many of them are (iTRAQ or TMT) or label-free relative quantification. The part of interdisciplinary research teams. The university has Materials Analysis Research Laboratory acquired a Fis- identified three focus areas for its biotechnology research, chione model 1060 ion mill for preparation of difficult including: samples. The milled surface is far superior to that possible by mechanical polishing allowing for the examination • increasing the value of current agricultural raw materials of fine structures. The Genome Informatics Facility hired through bioprocessing techniques two scientists to increase bioinformatics service capac- • increasing the efficiency, profitability and sustainability ity. The Biotechnology Outreach Education Center (BOEC) of plant and animal production and genetic modification provided supplies for biotechnology experiments to 120 of plants and animals Iowa schools, serving 12,970 students, and directly serv- • genetic modification of plants, animals and microbes ing 3,417 students through student visits to the Iowa State to produce more diverse products of greater economic campus or by visits at schools throughout the state. value. • 2017 – Dr. James Reecy was promoted from Director of the Office of Biotechnology to Associate Vice President for To help its new biotechnology researchers pursue these Research in the Office of the Vice President for Research. goals, start-up funds for 135 new researchers have been The Roy J. Carver Charitable Trust of Muscatine, Iowa, awarded through the biotechnology program. committed $192,600 to Iowa State University to expand x-ray crystallography capabilities for the univer- University-wide instrumentation facilities provide faculty sity. The Vice President for Research announced that Dr. and students from the university, other educational institu- Jeanne Serb would become the next director of the Office tions and industry scientists with access to state-of-the-art of Biotechnology, starting in this role January 1, 2018. instruments to support biotechnology research. See the The Biotechnology Outreach Education Center (BOEC) Services and Facilities section of this publication for details. provided supplies for biotechnology experiments to 113 Iowa schools, serving 10,232 students, and directly serv- Responsive Organization ing 3,377 students through student visits to the Iowa State The internal organization of the biotechnology program campus or by visits at schools throughout the state. allows quick responses to changing needs. The university- • 2018 – Dr. Jeanne Serb becomes the Director of the wide program is administered by the Office of Office of Biotechnology. The Plant Transformation Facil- Biotechnology, which is responsible to the Office of the ity moves under the administration of the Office of Bio- Vice President for Research. In 1984, the Office established technology and David Wright becomes the manager. The the Biotechnology Council which consists of faculty mem- Biotechnology Outreach Education Center (BOEC) provides bers with active research programs in biotechnology disci- supplies for biotechnology experiments to 85 Iowa schools, plines from the Colleges of Agriculture and Life Sciences, serving 8,550 students and directly serving 2,437 students Engineering, Human Sciences, Liberal Arts and Sciences through student visits to the Iowa State campus or by visits and Veterinary Medicine. The Office facilitates and advanc- at schools throughout the state. The DNA Facility purchases programs in research, education and outreach that con- es three new instruments – an Oxford Nanopore GridION tribute to the goals of Iowa State University’s Strategic Plan x5, a10X Genomics Chromium system and a BioTek in the area of Biotechnology. The Council recommends how

3 Research in Biotechnology 2020 funds available from the State of Iowa and other sources • Assisting in the location of industrial research and pro- could be used to obtain maximum benefit for biotechnology duction facilities in Iowa education, research and outreach. • Providing highly-trained scientists for the industrial work force Outreach Education The Office of Biotechnology conducts an education pro- The Office of Biotechnology has close working relation- gram for youth and adults that strives for clear explanations ships with the Iowa State Research Foundation, the Iowa and hands-on experiences with the science of biotechnol- State Office of Intellectual Property and Technology ogy and a balanced exploration of biotech’s risks, benefits Transfer, the Research Park Corporation, the Iowa Biotech- and ethical issues. The program’s financial sponsors have nology Association, the Iowa Department of Economic included the State of Iowa, commodity groups, indus- Development and chambers of commerce in the state. tries and individuals. Since it began in 1988, the program has developed teams of people from high schools, com- For further information about Iowa State’s technology trans- munity colleges, area education agencies and extension fer programs, contact Interim Director Dana Sue Rewoldt, offices throughout the state who can organize and conduct Telephone: 515-294-1041, E-mail: [email protected], biotechnology education programs for teachers and other Web: www.techtransfer.iastate.edu/. public educators. To Find Out More Public education activities continue to expand each year. The Office issues several publications that are available at Available services include: little or no cost. Many of these publications, news releases and other Iowa State biotechnology resources may be ac- • Free laboratory supplies, equipment and instructional cessed online at www.biotech.iastate.edu. materials for laboratory activities for Iowa K-12 schools, including Bt corn, soybean flavor and DNA extraction, Publications Available from the Office of Biotechnology fingerprinting and transformation To order the following publications, please contact us at: • The Biotechnology Outreach Education Center (BOEC), Iowa State University, Office of Biotechnology, 1210 Mo- a 2,300-square-foot laboratory and prep room facility lecular Biology Building, 2437 Pammel Drive, Ames, Iowa available to pre-service and K-12 teachers, extension 50011-1079; Telephone: 515-294-9818, E-mail: lorimill@ professionals and other educators to complete the same iastate.edu. The publications may be found online at www. experiments they will be teaching and to practice the lab biotech.iastate.edu. preparation required. K-12 students frequently visit the BOEC to experience hands-on activities in biotechnology, Research in Biotechnology — Annual directory of biotech- enhancing their STEM backgrounds. The BOEC has a full- nology faculty members and their research interests, plus time coordinator to assist educators and K-12 students an overview of the Biotechnology Program. Free. with instruction and educational development. Service Facilities for Biotechnology Research — Annual For more information about Iowa State’s biotechnology directory of research facilities on campus that offer biotech- outreach education program, please contact the BOEC at: nology-related instrumentation and resources. Free. Iowa State University, Biotechnology Outreach Education Center, Office of Biotechnology, 1210 Molecular Biology Iowa Biotech Educator — Electronic newsletter for biotech- Building, 2437 Pammel Drive, Ames, Iowa 50011-1079; nology educators that describes biotechnology educational Telephone: 515-294-9818, E-mail: [email protected], opportunities in Iowa, the content of educational programs, website: www.biotech.iastate.edu/for-k-14-educators/. classroom resources for educators and tips for teachers. Three issues per year. Free. Technology Transfer The State of Iowa and Iowa State University are committed Biotechnology E-News — Occasional electronic newsletter to economic development and public service by assisting in that contains brief articles about biotechnology research the transfer of scientific discoveries and technology to the and technology transfer developments at Iowa State. View marketplace. A responsibility of the Office of Biotechnology online or subscribe by sending an email with a request to is to support activities related to this mission. The key ele- be added to the E-News list to [email protected]. ments of technology transfer at Iowa State include: Biotechnology-Related Materials Available from Iowa • Making the resources of the university available to State Extension Distribution Center biotechnology companies To order the following publications, please visit the online • Establishing and promoting opportunities for research store at: http://store.extension.iastate.edu. collaboration between the biotechnology industries and the university • Promoting industrial investment in university product development and commercialization activities

4 Biotechnology Overview Curricula 4H 948 LDR – From Mendel to Markers - Impact of Mo- lecular Biology Technologies on Animal, Plant and Human Genetics. Grades 9-12, free download

Other PM 1988A – About Ethics - Bioethics Outreach Series, free download

5 Faculty Profiles Contact Information Faculty Profiles Research Interests Research Descriptions

Adelman, James S. Allenspach, Karin Natural Resource Ecology and Management Veterinary Clinical Sciences, Professor Assistant Professor 2512 Lloyd Small Animal Hospital 515-294-1281 201 Science Hall II 515-294-9682 Email: [email protected] Email: [email protected] Web: vetmed.iastate.edu/ Web: www.nrem.iastate.edu/people/james-adelman Drmedvet, Diplomate ECVIM-CA, Ph.D., University of Ph.D., Princeton University, 2010 Zurich, University of Berne, Switzerland, 1994 Research Interests: Animal physiology, evolutionary Research Interests: Biochemical engineering, veterinary biology, infectious diseases, immunobiology clinical trials Research Description: Influence of individual heterogene- Research Description: Investigation of the interplay of the ity on group- and population-level processes. Manner in intestinal mucosal innate immunity and epithelial cells with which variation in immune and behavioral responses to in- the microbiome. Use of intestinal organoids to investigate fection alter the transmission and evolution of an emerging interplay between host and microbiome. wildlife pathogen. Collaborates widely across all taxa with a special interest in songbirds. Ambrosio, Linda Roy J. Carver Department of Biochemistry, Biophysics and Al-Kaisi, Mahdi Molecular Biology; Associate Professor Agronomy, Professor 3264 Molecular Biology Building 515-294-9549 2303 Agronomy Hall 515-294-8304 Fax: 515-294-8457 Fax: 515-294-3163 Email: [email protected] Email: [email protected] Ph.D., Princeton University, 1985 Web: www.agronext.iastate.edu/smse/ Research Interests: Animal embryos, animal genetics, Ph.D., North Dakota State University, 1986 biochemical engineering, gene expression Research Interests: Soil-plant Interactions Research Description: Molecular mechanisms important Research Description: Effect of cropping systems, resi- for embryonic development and metamorphosis in due removal, nitrogen, and tillage management on soil car- insects. Investigation of enzyme mechanisms with a spe- bon dynamics, greenhouse gas emissions and soil quality. cial emphasis on the role of protein phosphorylation in animal developmental pathways essential to animal growth.

Allen, Heather K. Veterinary Microbiology and Preventive Medicine Anand, Robbyn Assistant Professor; USDA ARS, Collaborator Chemistry, Assistant Professor Food Safety and Enteric Pathogens Research Unit, NADC, 2101 C Hach Hall 515-294-8944 1920 Dayton Avenue, Ames, IA 50010 515-337-7207 Email: [email protected] Email: [email protected] Ph.D., University of Texas at Austin, 2010 Web: www.ars.usda.gov/pandp/people/people. Research Interests: Cancer, gene expression, live-cell htm?personid=44876 imaging, microfluidics, tissue engineering Ph.D., University of Wisconsin, 2009 Research Description: Detection and isolation of circulat- Research Interests: Antimicrobials, bioinformatics, food ing tumor cells. Dielectrophoretic technologies for manipu- safety, functional genomics, gene expression, metagenom- lation of cells (transport, trapping, sorting) in microfluidic ics, microbial genetics, microbial identification, microbial chips. Migration of cells in a tissue-on-chip environment, virulence, molecular genetics of microorganisms, nucleic especially as related to tumor metastasis. acids, systems biology, transposable elements Research Description: Alternatives to in-feed antibiotics in agricultural animals. Effect of in-feed antibiotics on the swine intestinal microbiome. Phage induction in commensal bacteria and in the foodborne pathogen Salmonella enterica serovar Typhimurium. Combining high-throughput sequence analysis with classical microbiological tools such as anaerobic culturing to address biological questions.

6 Research in Biotechnology 2020 Anantharam, Vellareddy Arias, Silvina Biomedical Science, Associate Professor Plant Pathology and Microbiology; PhD in Biochemistry 1107 Veterinary Medicine 515-294-6179 2115 Osborn Drive #164 5152940661 Fax: 515-294-315 Email: [email protected] Email: [email protected] Web: www.seeds.iastate.edu Web: vetmed.iastate.edu/users/anantram Ph.D. in Biochemistry, National University of Cordoba Ph.D., Indian Institute of Science, India, 1987 (Argentina), 2014 Research Interests: Animal cell culture, Big Data, bioin- Research Interests: Studies of interactions among plant formatics, brain research, clinical pharmacology, CRISPR/ pathogens, environmental conditions, and host defense Cas9, molecular pharmacology, nanotechnology, responses; plant-microbe associations; detection of neuroscience, pharmacokinetics, signal transduction, seedborne and seed-transmitted pathogens of crop plants; toxicology, transgenic animals analysis of mycotoxins and other secondary metabolites. Research Description: Cellular and molecular mechanisms Research Description: Study of risk associated with maize of neurodegeneration and the application of such informa- seed from plants infected with Xanthomonas vasicola pv. tion to the development of novel strategies for treating vasculorum (Xvv), the causal organism of the bacterial leaf Parkinson’s disease, prion disease and other neurodegen- streak (BLS), an emerging disease in corn in the U.S. erative diseases. Role of oxidative stress and endoplasmic Investigation of specific biochemical mechamisms related reticulum stress in etiopathogenesis of Parkinson’s and to the phytotoxic effects of Fumonisin B1 in tissues of prion diseases. maize seedlings, and the role of this mycotoxin in the ability of F. verticillioides to cause seedling disease.

Andreasen, Claire Veterinary Pathology, Professor; One Health, Director Arora, Rajeev 1790B Lloyd Veterinary Medical Building 515-294-0869 Horticulture, Professor Fax: 515-294-5423 139 Horticulture Hall 515-294 0031 Email: [email protected] Fax: 515-294-0730 DVM, Ph.D., Texas A & M University, University of Georgia, Email: [email protected] 1982, 1990 Ph.D., University of Wisconsin, 1990 Research Interests: Animal protection from infectious Research Interests: Plant physiology, protein structure and diseases, cytochemistry, food safety, immunobiology, function, stress response in plants stress response in animals, comparative pathology, Research Description: Physiology, molecular biology and diagnostics, One Health genetics of plant response to low-temperature stress, with Research Description: Granulocyte function and use of special emphasis on mechanism(s) of freeze injury, immunomodulators that normalize or upregulate cell func- recovery, cold acclimation and dormancy. tion in bacterial diseases. Assessment of granulocyte cell function and interaction with bacteria, especially in poultry during staphylococcal and Salmonella infections with and Aung, Kyaw without stress. Animal models to compare the clinicopath- Genetics, Development and Cell Biology; ologic and mechanistic pathogenesis of infectious dis- Assistant Professor eases. Comparative clinical pathology. Animal and human 3207 ATRB 5152949055 health – One Health. Email: [email protected] Ph.D., Michigan State University, 2011 Research Interests: Cell biology at the plant-microbe Andreotti, Amy H. interface Roy J. Carver Department of Biochemistry, Biophysics and Research Description: Understanding how plants harness Molecular Biology; University Professor intercellular communication to maintain homeostasis in 4208 Molecular Biology Building 515-294-4953 the regulation of growth and defense. Utilization of cellular, Fax: 515-294-0453 molecular, biochemical, and genetic approaches to uncover Email: [email protected] how pathogenic microbes manipulate plant PD to promote Ph.D., Princeton University, 1994 disease, using Arabidopsis-Pseudomonas syringae and Research Interests: Protein structure and function, Magnaporthe oryzae pathosystems. Using pathogenic structural biology, immunobiology microbes as probes, revealing the fundamental regulation Research Description: Macromolecular structure/function of cell-to-cell communication network in plants. Plant cell- relationships using multi-dimensional nuclear magnetic autonomous and non-cell-autonomous responses in the resonance (NMR) techniques. plant-microbe interface.

7 Faculty Profiles Bai, Hua Baum, David H. Genetics, Development and Cell Biology Veterinary Diagnostic and Production Animal Medicine Assistant Professor Senior Clinician 3260 Molecular Biology Building 515-294-9395 2656 Vet Med 515-294-1186 Email: [email protected] Fax: 515-294-3564 Web: www.thebailab.com Email: [email protected] Ph.D., University of Kentucky, 2009 Web: vetmed.iastate.edu/vdl Research Interests: Animal physiology, cell biology, gene MS, DVM, Ph.D., Iowa State University, 1978, 1983, 1997 expression, lipids, metabolomics, molecular genetics, Research Interests: Infectious diseases, immunobiology, muscle biology, proteomics, senescence, signal risk assessment transduction, stress response in animals Research Description: Observational studies for food Research Description: How animals age. Deciphering the animal production system’s analysis of antibody assay contributing factors (e.g. hormones) that regulate muscle results for improved animal productivity. aging.

Baum, Thomas J. Bannantine, John P. Plant Pathology and Microbiology Veterinary Microbiology and Preventive Medicine Charles F. Curtiss Distinguished Professor Assistant Professor 351 Bessey Hall 515-294-2398 USDA/ARS/National Animal Disease Center 515-337-7340 Fax: 515-294-9420 Fax: 515-337-7428 Email: [email protected] Email: [email protected] Web: www.baumlab.org Ph.D., Iowa State University, 1995 Ph.D., Clemson University, 1993 Research Interests: Animal protection from infectious Research Interests: Gene expression, molecular diseases, gene expression, genomics, microbial virulence, genetics of plants, plant-pest Interactions, signal monoclonal antibodies transduction, stress response in plants, transgenic plants Research Description: Identification and molecular char- Research Description: Molecular dissection of the com- acterization of specific proteins in Mycobacterium avium patible interaction between cyst nematodes and their subsp. paratuberculosis. This bacterium is a facultative hosts, with the goal to engineer novel plant resistance intracellular pathogen that causes Johne’s disease in cattle, mechanisms. sheep and other ruminant animals. The unique antigen(s) present only within the context of infection are the primary focus. Baumgard, Lance Animal Science, Associate Professor 313 Kildee Hall 515-294-3615 Bassham, Diane Fax: 515-294-3795 Genetics, Development and Cell Biology Email: [email protected] Loomis Professor of Plant Physiology Web: www.ans.iastate.edu/people/lance-baumgard 1035B Roy J. Carver Co-Lab 515-294-7461 Ph.D., Cornell University, 2001 Fax: 515-294-5256 Research Interests: Animal physiology, animal nutrition, Email: [email protected] lipids, stress response in animals, animal hormones and Web: www.gdcb.iastate.edu/people/diane-bassham peptide growth factors Ph.D., Warwick University, 1994 Research Description: Effect of thermal stress on post- Research Interests: Cell biology, gene expression, func- absorptive carbohydrate and lipid metabolism. Nutrient tional genomics, live-cell imaging, molecular genetics of partitioning in dairy cows and how dietary supplements plants, plant genetics, plant physiology, proteomics, signal alter feed efficiency in agriculturally important animals. transduction, stress response in plants, senescence, RNA Manipulation of fatty acid profiles in animal-derived food structure and function products and how this impacts nutritional value. Research Description: Plant stress responses. Signal perception and transduction during nutrient stress. Role of vacuolar autophagy in development and survival under stress conditions. Mechanisms of protein transport to the vacuole.

8 Research in Biotechnology 2020 Beattie, Gwyn A. Beitz, Donald Plant Pathology and Microbiology, Professor Animal Science, Distinguished Professor; Roy J. Carver 207 Science I 515-294-5571 Department of Biochemistry, Biophysics and Molecular Fax: 515-294-6019 Biology; Distinguished Professor Email: [email protected] 313 Kildee Hall 515-294-5626 Ph.D., University of Wisconsin, 1991 Fax: 515-294-3795 Research Interests: Gene expression, genomics, microbial Email: [email protected] genetics, microbial ecology, microbial virulence, molecu- Web: ans.iastate.edu/people/donald-beitz lar genetics of microorganisms, plant-pest interactions, Ph.D., Michigan State University, 1967 proteomics, stress response in microorganisms, stress Research Interests: Animal nutrition, human nutrition and response in plants, Big Data health, lipids, meat science and technology, vitamins and Research Description: Genomics and ecology of plant- minerals associated bacteria, bacterial plant pathology, role of plant Research Description: Dietary control of cholesterol microbiomes in plant drought stress tolerance, light-sens- homeostasis. Prevention and treatment of fatty liver and ing in Pseudomonas, metagenomes and microbiomes of ketosis in dairy cattle. Impact of nutrition and genetics on soybean plants. the composition and quality of animal products. Effect of probiotics and other dietary constituents on animal productivity and health. Beavis, William Agronomy and Plant Sciences Institute, Professor 1208 Agronomy and 1073 Carver Co-Laboratory Bellaire, Bryan 515-294-7301 Veterinary Microbiology and Preventive Medicine, Fax: 515-294-5267 Associate Professor and BSL3 Laboratory Director Email: [email protected] 1136 Veterinary Medicine 515-294-1006 Ph.D., Iowa State University, 1985 Fax: 515-294-8500 Research Interests: Genomics, functional genomics, Email: [email protected] translation of genomic discoveries Web: bbella.research.cvm.iastate.edu/ Research Description: Multi-objective optimization of Associate Professor, Louisiana State University, 2014 genetic improvement projects. Research Interests: Microbial virulence, animal protection from infectious diseases, microbial genetics, immunobiology, stress response in microorganisms, antimicrobials, Beck, Josh antibiotic resistance and imaging technology Biomedical Sciences Research Description: Host-pathogen interactions of 2052 Veterinary Medicine 515-294-8293 chronic bacterial and parasitic diseases. Understanding Email: [email protected] the microbial and cellular events that take place dur- Assistant Professor, UCLA, 2012 ing persistent infections. Basic research into pathogenic Research Interests: Apicomplexan parasite biology, mechanisms of Brucella, Francisella, Burkholderia, Yersinia malaria, membrane transport, protein trafficking and Mycobacterium species in a variety of hosts species, Research Description: Effector protein trafficking including humans. Translational approach to developing a Plasmodium parasites. novel anti-infective delivery platform to treat persistent and antimicrobial-resistant infections. Coordination of the College of Veterinary Medicine’s BSL3 Research Laboratory Facilities. Becraft, Philip W. Agronomy; Genetics, Development and Cell Biology, Professor; Associate Chair, Genetics, Development and Cell Biology Bentil, Sarah A. 2114 Molecular Biology Building 515-294-2903 Mechanical Engineering, Assistant Professor Fax: 515-294-7629 2104 Black Engineering Building 515-294-8528 Email: [email protected] Fax: 515-294-3261 Web: www.public.iastate.edu/~becraft/ Email: [email protected] Ph.D., University of California, 1992 Web: www.me.iastate.edu/sbentil/ Research Interests: Developmental biology, gene Ph.D., The Ohio State University, 2013 expression, genomics, molecular genetics of plants, plant Research Interests: Biomaterials, biomedical blast and genetics, signal transduction blunt impact injuries, brain-machine interfaces, engineer- Research Description: Molecular genetics of plant devel- ing, brain research, comparative pathology, computational opment and maize grain development. Cell interactions and biology, diagnostics, high-speed imaging, imaging technol- cell fate acquisition. Hormonal control of plant growth. ogy, neuroscience, non-invasive brain stimulation.

9 Faculty Profiles Research Description: Application of noninvasive experi- Blanchong, Julie mental techniques to characterize the material properties Natural Resource Ecology and Management of soft biological materials (e.g. brain) and biomaterials Associate Professor subjected to blast and blunt loading. Development of 339 Science II 515-294-9699 clinical diagnostic tools for assessing brain injury. Fax: 515-294-2995 Email: [email protected] Web: www.nrem.iastate.edu/people/julie-blanchong Bhattacharyya, Madan K. Ph.D., Michigan State University, 2003 Agronomy, Professor Research Interests: Animal genetics, epidemiology, G303 Agronomy Hall 515-294-2505 molecular genetics of animals, molecular ecology, molecu- Fax: 515-294-2299 lar genetics of microorganisms, vector-borne disease Email: [email protected] Research Description: Using genetics to characterize Web: www.agron.iastate.edu/people/madan-bhattacharyya relationships among host ecology and disease susceptibil- Ph.D., University of Western Ontario, 1987 ity, transmission, and distribution; identifying ecological and Research Interests: Functional genomics, genome map- environmental factors associated with disease outbreaks ping, genomics, metabolic engineering, metabolomics, and pathogen persistence; and evaluating impact of microfluidics, molecular genetics of microorganisms, disease to wildlife populations. molecular genetics of plants, plant genetics, plant-pest interactions, proteomics, transcriptome analysis, transgenic plants, transposable elements Blitvich, Bradley J. Research Description: Molecular basis of Phytophthora Veterinary Microbiology and Preventive Medicine resistance in soybean and nonhost resistance in Arabidop- Associate Professor sis and mechanism of sudden death syndrome in soybean 2116 Veterinary Medicine Building 515-294-9861 with a long-term goal to create wide-spectrum and durable Fax: 515-294-8500 resistance in soybean against Phytophthora sojae, Fusari- Email: [email protected] um virguliformae and other pathogens. Ph.D., University of Western Australia, 1997 Research Interests: Animal cell culture, animal protection from infectious diseases, gene expression, molecular Bigelow, Timothy A. virology, vector-borne disease, virology Electrical and Computer Engineering, Associate Professor Research Description: Arthropod-borne viruses (arbo- 255D Applied Science II 515-294-4177 viruses), genetic determinants that condition flavivirus Fax: 515-294-3637 host-range, impact of flaviviruses and orthobunyaviruses on Email: [email protected] human and veterinary health in Mexico, characterization of Ph.D., University of Illinois, Urbana, 2004 genes involved in apoptotic regulation in mosquito vectors. Research Interests: Biomedical engineering, cancer, developmental biology, drug detection, imaging technology, biorenewables Bobeck, Elizabeth Research Description: Improving the diagnostic and Animal Science, Assistant Professor therapeutic effectiveness of medical ultrasound. Applying 201F Kildee Hall please contact by email ultrasound to process microalgae for biofuel production. Email: [email protected] Quantifying the physical properties of tissue using back- Web: www.ans.iastate.edu/people/elizabeth-bobeck scattered ultrasound signals, applying ultrasound enhanced Ph.D., University of Wisconsin-Madison, 2012 drug/gene delivery to medical problems, exploring ultra- Research Interests: Animal cell culture, animal nutri- sound induced bioeffects for ultrasound safety and therapy tion, animal physiology, animal protection from infectious applications and developing ultrasound therapy techniques diseases, antibody diversity, antibody structure-function, to treat diseases. bioavailability of nutrients, cell biology, immunobiology, lipids, stress response in animals, vaccines Research Description: The intersection of immunology and nutrition, including characterization of lipid activation or down-regulation of immune system functions and effects on gut health and growth in poultry and related species. Mechanistic understanding of feed additives, gut health, and immune system activation. Targeted antibody engineering and neutraceutical production using laying hens. Improvement of poultry production through immune system modulation.

10 Research in Biotechnology 2020 Bobik, Thomas Brehm-Stecher, Byron Roy J. Carver Department of Biochemistry, Biophysics and Food Science and Human Nutrition, Associate Professor Molecular Biology; Professor 3344 Food Sciences Building 515-294-6469 2152 Molecular Biology Building 515-294-4165 Fax: 515-294-8181 Fax: 515-294-8247 Email: [email protected] Email: [email protected] Web: fshn.hs.iastate.edu/directory/profile. Ph.D., University of Illinois, Urbana, 1990 php?u=byron&embedded=true Research Interests: Bacterial genetics, bacterial Ph.D., University of Wisconsin, 2002 microcomponents, structural biology Research Interests: Food safety, food preservation and Research Description: Metabolism of vitamin B12 as it quality, antimicrobials, natural products, biomaterials, biore- relates to the treatment of human diseases associated newables, biosensors, nucleic acids, diagnostics, antibiotic with defects in the process. Production of commodity resistance, imaging technology, live-cell imaging, infectious chemicals from renewable resources. diseases, preanalytical sample preparation methods Research Description: Food safety. Rapid detection of foodborne pathogens and food spoilage organisms. Flow Boylston, Terri D. cytometry, image analysis and other single cell methods. Food Science and Human Nutrition, Associate Professor Antimicrobial development. Clean labels. 2547 Food Sciences Building 515-294-0077 Fax: 515-294-8181 Email: [email protected] Brewer, Matt Ph.D., Michigan State University, 1988 Veterinary Pathology, Assistant Professor Research Interests: Food preservation and quality, lipids, 2758 Vet Med 515-294-3090 flavor chemistry Email: [email protected] Research Description: The effects of processing and Web: brewer.research.cvm.iastate.edu/ storage on the lipid and flavor composition of foods, impact DVM, Ph.D., DACVM (Parasitology), Iowa State University, of probiotic bacteria on flavor quality of dairy products, 2012 development of starch-based products with increased Research Interests: Parasitic diseases, parasitic drug resis- resistant starch content. tance, alternative methods for control of parasitic disease Research Description: Investigation of parasite populations, drug resistance, diagnostic methods. Offers para- Bracha, Vlastislav sitology diagnostic services and can assist with animal Biomedical Sciences, Professor models of parasitic disease. 2032 Veterinary Medicine 515-294-6278 Fax: 515-294-2315 Email: [email protected] Bronikowski, Anne Web: vetmed.iastate.edu/users/vbracha Ecology, Evolution and Organismal Biology Ph.D., Czechoslovak Academy of Sciences, 1988 Associate Professor Research Interests: Animal physiology, human physiology, 237 Bessey Hall 515-294-7170 neuroscience Fax: 515-294-1337 Research Description: Neural substrates of learning and Email: [email protected] memory, movement control and goal-oriented behavior. Web: www.eeob.iastate.edu/faculty/BronikoA/homepage. html Ph.D., University of Chicago, 1997 Bratlie, Kaitlin Research Interests: Gene expression, genomics, Chemical and Biological Engineering, Associate Professor; molecular genetics of animals, senescence, stress Materials Science and Engineering, Associate Professor response in animals 2220 Hoover Hall 515-294-7304 Research Description: Evolutionary dynamics of loci that Fax: 515-294-5444 determine the form and rate of senescence. Genomic Email: [email protected] technologies used for study of physiological deterioration Web: kbratlie.public.iastate.edu with age and their pleiotropic effects on development and Ph.D., University of California, Berkeley, 2007 performance. Comparative biology of aging in the class Research Interests: Biomaterials, biomedical engineering, Reptilia, with emphasis on snakes and turtles. Physiological drug delivery, imaging technology, optical imaging experiments coupled with genome/transcriptome scans for Research Description: Understanding how polymer aging loci and expression regulation. properties influence host responses. In vivo imaging of collagen biosynthesis and innate immune cell secretions in response to implanted polymers. Non-linear optical techniques for examining host responses to polymers.

11 Faculty Profiles Brown, Robert Caston, Stephanie Gary and Donna Hoover Chair in Mechanical Engineer- Veterinary Clinical Sciences, Assistant Professor ing; Chemical and Biological Engineering, Anson Marston 2249 Lloyd Veterinary Medical Center 515-294-1500 Distinguished Professor of Engineering; Agricultural and Email: [email protected] Biosystems Engineering Web: vetmed.iastate.edu/users/scaston 1140 Biorenewables Research Laboratory 515-294-7936 DVM, DACVS - LA, Texas A&M University, 2002 Fax: 515-294-3091 Research Interests: Animal cell culture, cancer, Email: [email protected] immunobiology, STEM education Web: www.me.iastate.edu/directory/faculty/robert-c- Research Description: Evaluation of CD47 expression in brown/ veterinary patient tumors and development of immuno- Ph.D., Michigan State University, 1980 therapy utilizing anti-CD47 antibodies. Veterinary student Research Interests: Biorenewables teaching, including clinical skills using simulators and Research Description: Thermochemical and hybrid development of a clinical skills laboratory focusing on conversion of biomass into fuels, chemicals and energy. independent learning with virtual or live mentoring/ Thermal deconstruction of biomass into solubilized car- instruction, self-directed practice, and remediation. bohydrate, phenolic oil and acetate using pyrolysis and solvent liquefaction. Fermentation of syngas derived from biomass gasification into ethanol and other biobased prod- Chang, Carl K. ucts. Solvent liquefaction of technical lignin from cellulosic Computer Science, Professor and Director/ ethanol plants into high value aromatic chemicals. Carbon Software Engineering negative energy technologies to remove carbon from the 226 Atanasoff 515-294-4377 atmosphere and sequester it in geological deposits (as Fax: 515-294-0258 carbon dioxide) or soils (as biochar). Email: [email protected] Web: www.cs.iastate.edu/~chang Ph.D., Northwestern University, 1982 Brumm, Thomas J. Research Interests: Brain research, human physiology, Agricultural and Biosystems Engineering STEM education Associate Professor Research Description: Understanding human perception 1328 Howe Hall 515-294-5145 and applying brain informatics to human intention detection Fax: 515-294-6184 and software services evolution. Email: [email protected] Ph.D., Iowa State University, 1990 Research Interests: Biomaterials, biorenewables Charavaryamath, Chandrashekhar Research Description: Storage of biorenewable materials; preservation, degradation, enhancement, and integration (Chandru) into biorefineries. Biomedical Sciences, Assistant Professor 1044 Veterinary Medicine 515-294-7710 Email: [email protected] BVSc, MVSc, Ph.D., University of Agricultural Sciences, Carlson, Steve University of Saskatchewan, Saskatoon, SK, Canada, Biomedical Sciences, Associate Professor Research Interests: Innate inflammation in the lung and 2028 Veterinary Medicine 515-294-0912 brain and gut mucosal immunity Fax: 515-294-2315 Research Description: Understanding the mechanisms Email: [email protected] of organic dust exposure induced airway and neuroinflam- DVM, Ph.D., Iowa State University, University of Iowa, mation, role of mitochondria and endoplasmic reticulum in 1990, 1997 inflammation and gut mucosal immunity. Research Interests: Antimicrobials, food safety, microbial genetics, microbial virulence, neuroscience, pharmacokinetics, signal transduction, vaccines, antibiotic resistance Research Description: Identification of novel antimicrobial agents. Identification of biocontrol measures that promote food safety. Identification of Salmonella genes involved in virulence and antibiotic resistance. Identification of etiopathogenic mechanisms involved in Salmonella toxic encephalopathies. Identification of protozoal receptors involved in bacterial engulfment.

12 Research in Biotechnology 2020 Chen, Stone Ciardo, Gianfranco Roy J. Carver Department of Biochemistry, Biophysics and Computer Science, Professor Molecular Biology; Assistant Professor 105 Atanasoff Hall 515-294-3264 3110 Molecular Biology Building 515-294-9891 Email: [email protected] Email: [email protected] Web: web.cs.iastate.edu/~ciardo/ Web: www.stonechenlab.org/ Ph.D., Duke University, 1989 Ph.D., ISU, 2008 Research Interests: Bioinformatics Research Interests: Animal cell culture, biochemical Research Description: Genome assembly algorithms. engineering, bioinformatics, cancer, cell adhesion, cell biology, CRISPR/Cas9, enzyme mechanisms, live-cell imaging, molecular machines, molecular mechanisms, molecular Claussen, Jonathan pharmacology, neuroscience, protein engineering, protein Mechanical Engineering, Assistant Professor structure and function, protein 2104 Black Engineering 515-294-4690 structure prediction, proteomics, signal transduction Email: [email protected] Research Description: Regulation of the actin cytoskel- Web: web.me.iastate.edu/claussen/ eton. Ph.D., Purdue University, 2011 Research Interests: Biomedical engineering, biosecurity, food safety, microfluidics, nanotechnology Cho, Michael W. Research Description: Understanding/developing science Biomedical Sciences, Professor and technology that interfaces with biology on the nano/mi- 2022 Veterinary Medicine 515-294-6449 croscale. Developing carbon nanomaterials (e.g., graphene, Fax: 515-294-2315 carbon nanotubes, carbon dots) and hybrid nanostructured Email: [email protected] materials for disease diagnostics/treatment, energy, mo- Ph.D., University of Utah, 1994 lecular logic, and small-scale propulsion applications. Use Research Interests: Antiviral agents, bioinformatics, of biological agents (enzymes, antibodies, DNA, cells) and biomedical engineering, carbohydrates, cell biology, DNA surface chemistry techniques. Understanding and model- structure and function, floral biology, gene expression, ing the reaction-diffusion kinetics associated with the nano- immunobiology, microbial virulence, molecular virology, bio interface, requiring expertise from nearly all engineering monoclonal antibodies, nanotechnology, protein and science backgrounds. engineering Research Description: Structure-function analyses of viral envelope glycoproteins. Virus-host interactions, especially Clavijo, Maria, J. the molecular mechanism of virus entry. Development of Veterinary Diagnostic and Production Animal Medicine, viral vaccines and virus entry inhibitors, and characteriza- Research Assistant Professor tion of host immune responses (humoral immunity, in par- 2201 Lloyd Veterinary Medicinen 612-868-4396 ticular). Development of vaccine delivery platforms, includ- Email: [email protected] ing viral and bacterial vectors, and nanoparticles. Primary Web: vetmed.iastate.edu/users/mclavijo areas of research are HIV-1 and influenza A virus. DVM, Ph.D., Universidad Central de Venezuela (DVM), University of Minnesota (PhD), 2009, 2014 Research Interests: Antibiotic resistance, antimicrobials, Chou, Li-Shan biosecurity, diagnostics, epidemiology, microbial ecology, Kinesiology, Professor and Chair microbial identification, microbial virulence, molecular ecol- 235A Forker Building 515-294-1425 ogy, molecular epidemiology Email: [email protected] Research Description: Infectious diseases of swine, epi- Ph.D., University of Illinois at Chicago, 1995 demiology and diagnostics. Research Interests: Biomechanics, rehabilitation engineering, human movement analysis Research Description: Interactions among the muscular Coats, Joel R. skeletal system, the brain and central nervous system, and Entomology, Distinguished Professor the sensory system when presented with injuries, deficits, 116 Insectary 515-294-4776 or interventions, and how they affect outcomes of human Fax: 515-294-7406 movement. Email: [email protected] Ph.D., University of Illinois, 1974 Research Interests: Insect toxicology, natural products, environmental toxicology and chemistry Research Description: Study of naturally occurring toxins and their effects on insects, including growth, development, repellency, behavior and mortality. Investigation of

13 Faculty Profiles microbial degradation of toxic substances and environmen- Dai, Xiongtao tal fate and effects of agrochemicals. Statistics, Assistant Professor Snedecor 2220 515-294-2182 Email: [email protected] Cochran, Eric W. Web: xdai.public.iastate.edu/ Chemical and Biological Engineering, Associate Professor Ph.D., University of California, Davis, 2018 3155 Sweeney Hall 515-294-0625 Research Interests: Longitudinal data analysis, plant Fax: 515-294-2689 genomics Email: [email protected] Research Description: Development and application of Web: www.cbe.iastate.edu/the-department/ statistical methods for analyzing longitudinal data in biologi- facultystaff/?user_page=ecochran cal sciences. Modeling population dynamics of repeated Ph.D., University of Minnesota, 2004 elements in plant genomes. Research Interests: Biomaterials, biorenewables, nanotechnology, plant protein and oil utilization Research Description: Thermoplastic polymers, elasto- Day, Tim A. mers, and copolymers derived from biofeedstocks includ- Biomedical Sciences, Associate Professor ing vegetable oils, algal oil, fast pyrolysis residues and 2008 Veterinary Medicine 515-294-7100 biodiesel residues. Fax: 515-294-2315 Email: [email protected] Web: www.public.iastate.edu/~day/ Coffman, Clark Ph.D., Michigan State University, 1992 Genetics, Development and Cell Biology Research Interests: Animal physiology, bioinformatics, Assistant Professor cell biology, comparative genomics, muscle biology, signal 3258 Molecular Biology Building 515-294-3911 transduction Email: [email protected] Research Description: Biology of parasitic worms and the Web: www.public.iastate.edu/~ccoffman/ identification of novel drug targets in parasitic worms, with Ph.D., University of California, San Diego, 1993 an emphasis on the nervous system and the role that neu- Research Interests: Animal genetics, animal embryos, ropeptides play in parasite neurobiology. Also, the emer- cancer, cell biology, developmental biology, signal gence of resistance to antiparasitic drugs used to control transduction, STEM education infections in humans. Internal bone forces. Research Description: Investigation of the regulation of cell migration and programmed cell death using Drosophila germ cells as a model for normal developmental events, as Dekkers, Jack C. M. well as metastasis. Mechanisms used by cells to pathfind Animal Science, Distinguished Professor and move within an organism. How cells that are mis- 239D Kildee Hall 515-294-7509 placed or in excess are targeted for elimination. Improving Fax: 515-294-9150 student engagement and learning in large classrooms, Email: [email protected] student motivation for learning and student retention. Web: www.ans.iastate.edu/people/jack-c-dekkers Ph.D., University of Wisconsin, 1989 Research Interests: Animal breeding, animal genetics, Cunnick, Joan E. quantitative genetics, genomics, bioinformatics Animal Science, Professor Research Description: Quantitative genetics and animal 207 Science I 515-294-2070 breeding, including use of molecular genetic information in Fax: 515-294-6019 selection programs, QTL detection, and genetics of Email: [email protected] disease and feed efficiency. Web: www.ans.iastate.edu/people/joan-cunnick Ph.D., Kansas State University, 1987 Research Interests: Immunobiology, stress response in animals, vaccines Research Description: Edible vaccines. Immune modulation in various animal species, directed at understanding the mechanisms by which stress (psychoneuroimmunol- ogy) or toxicants can alter and suppress immune function. Nutrients or foods which can increase immune function and the mechanisms of action.

14 Research in Biotechnology 2020 Derrick, Timothy R. Dickson, James S. Kinesiology, Professor Animal Science, Professor 249 Forker Building 515-294-8438 215F Science I 515-294-4733 Fax: 515-294-8740 Fax: 515-294-6019 Email: [email protected] Email: [email protected] Ph.D., University of Massachusetts, 1996 Web: www.ans.iastate.edu/people/james-dickson Research Interests: Biomedical engineering, structural Ph.D., University of Nebraska, Lincoln, 1984 biology Research Interests: Food preservation and quality, food Research Description: Calculation of internal bone forces processing, food safety, meat science and technology, in humans. Transmission of shock waves in the human microbial identification, microbial virulence, risk assess- body. Injuries to the body that result from impacts or asyn- ment, stress response in microorganisms chronous joint motion. Kinematic and kinetic analysis of Research Description: Food preservation and quality. human motion.

DiSpirito, Alan A. Derscheid, Rachel J. Roy J. Carver Department of Biochemistry, Biophysics and Veterinary Diagnostic and Production Animal Medicine Molecular Biology; Professor Assistant Professor 4114 Molecular Biology Building 515-294-2944 1815 Veterinary Medicine Annex 515-294-1173 Fax: 515-294-0453 Email: [email protected] Email: [email protected] Ph.D., DVM, Iowa State University, 2012 Ph.D., The Ohio State University, 1983 Research Interests: Mycoplasma of veterinary impact, Research Interests: Bioremediation of environmental comparative pathology, infectious diseases, vaccines pollutants, use of methanobactin in the treatment of cop- Research Description: Collaborative research on diseases per related diseases, enzyme mechanisms, fermentation, of livestock, particularly swine, with focus on Mycoplasma metalloproteins, molecular genetics of microorganisms, spp., including pathogenesis, diagnostic assay and tool protein structure and function, metal binding compounds development, and prevention and treatment. Pathogenesis Research Description: Mechanism of methane oxidation of livestock diseases with emphasis on pathogen-host in methanotrophs. Mechanism of electron flow between interaction and prevention of disease. the oxidative reactions and electron transport chain. Evaluation of the use of methanotrophs in bio-remediation processes. Determination of the rates and mechanism(s) of Dickerson, Julie A. oxidation of halogenated hydrocarbons by methanotrophs. Electrical and Computer Engineering Medical, environmental uses of methanobactin. Northrop Grumman Professor 3123 Coover Hall 515-294-7705 Fax: 515-294-8432 Dixon, Philip Email: [email protected] Statistics, University Professor Web: home.eng.iastate.edu/~julied/ 2121 Snedecor Hall 515-294-2142 Ph.D., University of Southern California, 1994 Fax: 515-294-4040 Research Interests: Bioinformatics, comparative genom- Email: [email protected] ics, computational biology, gene expression, metabolic Web: pdixon.stat.iastate.edu engineering, metabolomics, signal transduction, systems Ph.D., Cornell University, 1986 biology, Big Data Research Interests: Biostatistics, metabolomics, risk Research Description: Modeling metabolic networks assessment in plants and bacteria. Applications of data visualization, Research Description: Statistical analysis of complex machine learning, and virtual reality to better understand biological data. Modeling in metabolomic data. Quantitative high-throughput “omics” data. Database development for risk assessment. gene expression and metabolomics datasets. Data analysis and integration for cross-species and cross-experiment comparisons.

15 Faculty Profiles Dong, Liang Research Description: Genetic factors and mechanisms Electrical and Computer Engineering, Assistant Professor underlying selection response in elite maize breeding 2115 Coover Hall 515-294-0388 populations. Application of translational genomics and sta- Fax: 515-294-8432 tistical tools in plant breeding programs. Email: [email protected] Ph.D., Tsinghua University, 2004 Research Interests: Biomedical engineering, biomaterials, Ellinwood, N. Matthew drug detection, biorenewables, food safety, nanotechnol- Animal Science, Veterinary Clinical Sciences ogy, imaging technology, tissue engineering Associate Professor Research Description: Development of miniature lab-on- 2356D Kildee Hall 515-294-5136 chip devices and systems for studying influence of chemi- Fax: 515-294-4471 cal/biological/mechanical/electrical/optical/electromagnetic Email: [email protected] environments on single cells and multicellular organisms, Web: www.ans.iastate.edu/people/matthew-ellinwood e.g., plants or nematodes. D.V.M., Ph.D., Colorado State University, Colorado State University, 1997, 2000 Research Interests: Reproductive physiology, animal cell Dorman, Karin S. culture, animal genetics, animal physiology, biological com- Statistics, Genetics, Development and Cell Biology pound separation and detection, carbohydrates, catalysis, Professor enzyme mechanisms, gene expression, genomics, 2411 Snedecor Hall, 534 Science II 515-294-1457 molecular genetics of animals, neuroscience Fax: 515-294-4040 Research Description: The genetics, pathology, and Email: [email protected] treatment of neuropathic disorders, specifically lysosomal Ph.D., University of California, Los Angeles, 2001 storage disorders using spontaneous large animal models Research Interests: Bioinformatics, computational biology, of human diseases. genomics, molecular evolution, virology, systems biology Research Description: Developing probability and statistical models for biological data, with specific interest in Ellis, Timothy G. molecular evolution, especially virus evolution; next genera- Civil, Construction and Environmental Engineering tion sequencing, especially error modeling and correction; Associate Professor phylogenetic methods; epigenetics, especially CpG meth- 375 Town Engineering 515-294-8922 ylation; and all kinds of genetic sequence analysis. Fax: 515-294-8216 Email: [email protected] Web: www.public.iastate.edu/~tge/ Dutta, Somak Ph.D., Clemson University, 1995 Statistics, Assistant Professor Research Interests: Bioreactors, bioremediation of 2409 Snedecor Hall 515-294-4998 environmental pollutants, waste management Email: [email protected] Research Description: Use of biological systems for Ph.D., University of Chicago, 2015 treatment of municipal, industrial and agricultural wastes Research Interests: Gene expression, factor models, for by-product recovery and environmental restoration. significance tests, clinical trial, dose-response models, Evaluation of biodegradation kinetics using respirometry. regression and QTL mapping, Recovery of valuable fertilizer components from agricultural Research Description: Factor models, statistical wastes using struvite precipitation. Evaluation, design and computations, spatial statistics, high-dimensional methods. operation of odor control facilities. Biomethane production from municipal, agribusiness and industrial wastestreams. Upset early warning detection using whole cell microbial Edwards, Jode biosensors. Agronomy, Assistant Professor; USDA ARS CICG Collaborator 1503 Agronomy Hall 515-294-7607 Fax: 515-294-9359 Email: [email protected] Web: www.ars.usda.gov/pandp/people/people. htm?personid=35607 Ph.D., Iowa State University, 1999 Research Interests: Biostatistics, computational biology, genomics, plant breeding, quantitative genetics

16 Research in Biotechnology 2020 Espin Palazon, Raquel Research Interests: Molecular genetics of plants, plant Genetics, Development and Cell Biology breeding, plant genetics, stress response in plants, Assistant Professor transgenic plants 2213 Pammel Drive; Room 3003 515-294-4628 Research Description: Turfgrass germplasm evaluation Email: [email protected] and enhancement, molecular biology of abiotic stress University of Murcia, Spain, 2013 tolerance in perennial grasses, genetic transformation and Research Interests: Normal and diseased hematopoiesis, molecular marker studies. hematopoietic stem cells, blood development, Inflamma- tory signals, zebrafish, single cell biology Research Description: The improvment of human health Fernández-Baca, David through the identification of targets and novel therapies Computer Science, Professor to advance regenerative medicine to treat blood disorders 209 Atanasoff Hall 515-294-2168 such as myelodysplasia and leukemia. Deciphering the cel- Fax: 515-294-0258 lular and molecular mechanisms that lead to blood forma- Email: [email protected] tion. Zebrafish as animal vertebrate model to study how Web: www.cs.iastate.edu/~fernande/ blood is created during embryo development in vivo. This Ph.D., University of California, Davis, 1986 unique model provides the entire context of an organism, Research Interests: Bioinformatics, computational biology, that allows for blood formation deciphering. Once under- evolutionary biology stood, in vitro experiments with human cells in combina- Research Description: Construction of phylogenetic trees, tion with bioinformatics tools and single cell biology are supertree methods, clustering of phylogenic data, phyloge- utilized for the translation of the findings to human health. netic databases.

Essner, Jeffrey J. Fernando, Rohan L. Genetics, Development and Cell Biology, Professor Animal Science, Professor 3007 Advanced Teaching and Research Building 237 Kildee Hall 515-294-5348 515-294-7133 Fax: 515-294-9150 Fax: 515-294-8457 Email: [email protected] Email: [email protected] Web: www.ans.iastate.edu/people/rohan-fernando Web: www.gdcb.iastate.edu/people/jeffrey-essner Ph.D., University of Illinois, Urbana, 1984 Ph.D., University of Minnesota, 1996 Research Interests: Animal breeding, animal genetics, Research Interests: Animal embryos, cancer, cell biology, genome mapping developmental biology, gene editing, signal transduction, Research Description: Development of statistical meth- transgenic animals, transposable elements ods and computer algorithms for mapping quantitative trait Research Description: Use of zebrafish to interrogate loci and for incorporating genetic marker data in genetic vascular and tube morphogenesis using gene editing evaluation and selection. technologies.

Friedberg, Iddo Eulenstein, Oliver Veterinary Microbiology and Preventive Medicine Computer Science, Professor Associate Professor 212 Atanasoff Hall 515-294-2407 2118 Vet Med 515-294-5959 Fax: 515-294-4377 Email: [email protected] Email: [email protected] Web: iddo-friedberg.net Web: www.cs.iastate.edu/~oeulenst/ Ph.D., Hebrew University of Jerusalem, 2003 Dr. rer. nat., Bonn University, Germany, 1998 Research Interests: Bioinformatics, evolutionary biology, Research Interests: Computational biology, bioinformatics genomics, metagenomics, structural biology, protein struc- Research Description: Algorithms for problems in ture and function, microbial genetics, microbial ecology molecular biology. Research Description: Description of the evolution of operons and gene blocks in bacteria. Development of a molecular clock to discern the rate of evolution of gene Fei, Shui-zhang blocks. Automated computational protein function predic- Horticulture, Associate Professor tion and collaboration regarding it. Correlation between the 257 Horticulture Hall 515-294-5119 human gut microbiota and gut gene expression. Infant gut Fax: 515-294-0730 development and the effect of gut microbes on human Email: [email protected] health and wellness. Ph.D., University of Nebraska, Lincoln, 1997

17 Faculty Profiles Gabler, Nicholas Gassmann, Aaron J. Animal Science, Associate Professor Entomology, Associate Professor 201 Kildee Hall 515-294-7370 18 Insectary Building 515-294-7623 Fax: 515-294-1399 Fax: 515-294-7406 Email: [email protected] Email: [email protected] Web: www.ans.iastate.edu/people/nicholas-gabler Web: www.ent.iastate.edu/dept/faculty/gassmann/ Ph.D., La Trobe University, Australia, 2005 Ph.D., State University of New York, Stony Brook, 2003 Research Interests: Swine nutrition, intestinal physiology, Research Interests: Economic implications, evolutionary cell biology, developmental biology, metabolism, stress and biology, food biotechnology, integrated pest management, disease response in swine plant-pest interactions Research Description: Molecular mechanisms by which Research Description: Study of interactions between nutrition and immune system activation alter growth and agricultural pest insects and genetically-engineered crops intestinal function. Investigation of the molecular aspects that produce insecticidal toxins derived from the bacterium of feed efficiency in pigs with an emphasis on feed intake Bacillus thuringiensis (Bt). Ecological and genetic factors regulation, skeletal muscle synthesis/degradation pathways that affect the evolution of Bt resistance in insects. and metabolism. Characterization of molecular and physiological impact of environmental stressors such as nutrition, disease and heat stress on gastrointestinal integrity, Gauger, Phillip function and metabolism. Veterinary Diagnostic and Production Animal Medicine Associate Professor 1575 Veterinary Medicine 515-294-2437 Gallagher, Paul W. Email: [email protected] Economics, Associate Professor Web: vetmed.iastate.edu/users/pcgauger 568D Heady Hall 515-294-6181 DVM, Ph.D., Iowa State University, 1994, 2012 Fax: 515-294-3838 Research Interests: Comparative pathology, molecular Email: [email protected] diagnostics, molecular virology, vaccines, virology Ph.D., University of Minnesota, 1983 Research Description: Pathogenesis of infectious dis- Research Interests: Economic implications eases of production animals and development of diagnostic Research Description: Conducts research through the tests. Center for Crops Utilization Research.

Gillette, Jason C. Gardner, Candice Kinesiology, Associate Professor Agronomy, Collaborator 245 Forker Building 515-294-8310 G212 Agronomy 515-294-7967 Fax: 515-294-8740 Fax: 515-294-4880 Email: [email protected] Email: [email protected] Ph.D., Iowa State University, 1999 Ph.D., University of Missouri-Columbia, 1982 Research Interests: Biomedical engineering, risk assess- Research Interests: Biorenewables, biosecurity, doubled ment, human physiology haploids, plant breeding, plant genetics Research Description: Using video, force, and EMG Research Description: Plant genetic resource conserva- measurements to analyze human motion, along with mus- tion and utilization. Use of technology to identify, utilize, culoskeletal modeling to estimate internal loading on the and adapt unique plant genetic resources to support plant structures of the human body. Athletic movements such as research objectives. Develop and provide doubled haploid jump landings and running, along with activities of daily liv- maize inbred genetic resources to support research objec- ing such as walking, sit-to-stand, and stair negotiation. ACL tives. Use of biotechnology to better support plant genetic injury mechanisms and the pathway of knee degeneration resource conservation. from ACL and meniscal injury in young adults to development of osteoarthritis in older adults. Assessment of exoskeleton use in industrial settings to prevent shoulder fatigue and chronic overuse injuries.

18 Research in Biotechnology 2020 Girton, Jack R. Gordon, Mark Roy J. Carver Department of Biochemistry, Biophysics and Chemistry, Distinguished Professor Molecular Biology; University Professor 201 Spedding Hall 515-294-0452 3152 Molecular Biology Building 515-294-0342 Fax: 515-294-5204 Fax: 515-294-0345 Email: [email protected] Email: [email protected] Web: www.msg.ameslab.gov Ph.D., University of Alberta, 1979 Ph.D., Carnegie Mellon University, 1968 Research Interests: Animal genetics, developmental Research Interests: Computational biology, enzyme biology, gene expression, molecular genetics of animals, mechanisms transposable elements Research Description: Examination of function, structure Research Description: Structure and function of chromo- and reactivity using quantum chemistry. Quantum mechan- somes in Drosophila. Regulation of gene expression by ics combined with model potentials. modification of histome and nonhistome chromosome proteins. Epigenetic impacts of modification of chromosomal proteins. Genetic control of the mitotic spindle matrix. Grant, David Identity, structure and function of spindle matrix genes. Us- Agronomy, Affiliate Associate Professor ing Drosophila P type transposable element transformation G304 Agronomy Hall 515-294-1205 vectors to determine the function of components of genes. Email: [email protected] Web: www.agron.iastate.edu/people/david-grant Ph.D., University of Chicago, 1977 Gleason, Mark L. Research Interests: Bioinformatics, comparative genom- Plant Pathology and Microbiology, Professor ics, genomics, molecular genetics of plants, evolutionary 313 Bessey Hall 294-0579 biology, plant genetics Fax: 515-294-9420 Research Description: Evolution of genome structure in Email: [email protected] plants after complete or segmental genome duplication. Ph.D., University of Kentucky; University of Virginia, 1985; Use bioinformatics tools to study gene content, gene func- 1980 tion and mRNA processing in soybean. Web applications Research Interests: Phylogenetic analysis and genomics for presentation of genomic and genetic data. of phytopathogenic fungi and bacteria Research Description: Identification of species of fungal and bacterial pathogens of fruit and vegetable crops, Greenlee, Heather including sooty blotch and flyspeck (SBFS) of apple, Biomedical Sciences, Associate Professor anthracnose fruit rot (AFR) of strawberry, and bacterial wilt 2070 Vet Med 515-294-9251 of cucurbits, among others. Genomics of pathogen adapta- Fax: 515-294-2315 tions to environment and host, focusing on SBFS, AFR, and Email: [email protected] bacterial wilt. Ph.D., Iowa State University, 1999 Research Interests: Comparative pathology, infectious diseases, non-invasive diagnostics Goggi, A. Susana Research Description: Using the retina to develop non- Agronomy, Professor invasive diagnostics for protein misfolding neurodegenera- 195C Seed Science (515) 294-6372 tive disorders. Email: [email protected] Ph.D., Mississippi State University, 1990 Research Interests: Seed physiology, abiotic and biotic Greiner, Laura stress effects on seed Animal Science; Assistant Professor Research Description: Physiological aspects of seed 109C Kildee, 806 Stange Rd 5152946728 development, maturation, longevity, dormancy, and ger- Email: [email protected] mination. Strategies for understanding and minimizing the Assistant Professor, Iowa State University, 2001 adverse effects of the production environments on seed Research Interests: Swine nutrition and health interactions quality of soybeans and corn. Phenotypic and molecular study of environmental stress-induced soybean genome fluidity events affecting seed quality and vigor.

19 Faculty Profiles Gu, Xun of microorganisms, molecular plant-microbe interactions, Genetics, Development and Cell Biology stress response in microorganisms Associate Professor Research Description: Identification of molecular mecha- 503 Science II 515-294-8075 nisms of bacterial and algal adaptation to environmental Email: [email protected] stress, including how bacterial-algal interactions facilitate Ph.D., University of Texas, 1996 algal stress tolerance. Examining the integration of stress Research Interests: Computational biology, genome adaptation mechanisms and bacterial biofilm developmen- mapping, plant genetics tal processes on fitness. Investigations into soil and plant Research Description: Understanding the functional diver- microbiomes and how cropping systems and plant geno- gence between homologous genes within a gene family. types influence microbiome structure and function. Identification of important amino acid residues for these functional differences. Applications in functional genomics. Hannapel, David J. Horticulture, Professor Gupta, Mohan 253 Horticulture Hall 515-294-9130 Genetics, Development and Cell Biology Fax: 515-294-0730 Assistant Professor Email: [email protected] 457 Bessey Hall 515-294-3951 Ph.D., Purdue University, 1985 Fax: 515-294-1337 Research Interests: Developmental biology, gene expres- Email: [email protected] sion, molecular genetics of plants, plant breeding, plant Web: www.guptalab.science genetics, RNA structure and function Ph.D., Iowa State University, 2001 Research Description: Characterization of regulatory Research Interests: Cancer, cell biology, live-cell imaging, genes involved in controlling plant growth. protein structure and function Research Description: Define the molecular mechanisms that control microtubule dynamics during diverse cellular Hansen, Stephanie L. processes. Understand how defects in microtubule dynam- Animal Science, Associate Professor ics cause neurological disease and can be leveraged for 313F Kildee Hall 515-294-7326 improved cancer treatments. Experimental approaches Fax: 515-294-3795 include cell biology, biochemistry, genetics, biophysics, and Email: [email protected] high-resolution microscopy with special emphasis on the Web: www.ans.iastate.edu/hansen-ruminant-nutrition-lab budding yeast model system, Saccharomyces cerevisiae. Ph.D., North Carolina State University, 2008 Research Interests: Animal nutrition, bioavailability of nutrients, gene expression, vitamins and minerals Halbur, Patrick G. Research Description: Ruminant nutrition, emphasis on Veterinary Diagnostic and Production Animal Medicine mineral metabolism, including molecular mechanisms re- Professor and Chair sponsible for absorption and transport of minerals, as well 2203A Lloyd Veterinary Medical Center 515-294-6970 as the interactions between trace elements. Email: [email protected] Web: scholar.google.com/citations?user=TmyQSOkAAAA J&hl=en Hargrove, Mark DVM, Ph.D., Iowa State University, 1986, 1995 Roy J. Carver Department of Biochemistry, Biophysics and Research Interests: Animal protection from infectious Molecular Biology; Professor diseases, molecular virology, vaccines, virology 4116 Molecular Biology Building 515-294-2616 Research Description: Pathogenesis, diagnosis, treat- Fax: 515-294-0453 ment of and protection against swine and bovine infectious Email: [email protected] diseases. Epidemiology of zoonotic diseases. Web: www.bbmb.iastate.edu/people/mark-hargrove Ph.D., Rice University, 1996 Research Interests: Biorenewables, enzyme mechanisms, Halverson, Larry metalloproteins, protein structure and function Plant Pathology and Microbiology, Associate Professor Research Description: Protein structure and function. 304 Science I 515-294-0495 Use of X-ray crystallography and kinetic methods to study Email: [email protected] hemoglobins from plants, animals and bacteria in an effort Web: www.plantpath.iastate.edu/ to understand how this protein fold and prosthetic group Ph.D., University of Wisconsin-Madison, 1991 dictates different functions in different organisms. Design Research Interests: Biorenewables, biosensors, gene of cellulases based on cellulosomes found in anaerobic expression, metabolic engineering, microbial ecology, bacteria. Use of biochemical methods to assemble large microbial genetics, molecular ecology, molecular genetics enzymes with multiple hydrolases for the development of enzymes for cellulose degradation. 20 Research in Biotechnology 2020 Harmon, Karen M. Heindel, Theodore (Ted) J. Veterinary Diagnostic and Production Animal Medicine, Mechanical Engineering, University Professor and Senior Clinician Bergles Professor of Thermal Science 1573 Veterinary Medicine 515-294-5184 2644 Howe Hall 515-294-0057 Email: [email protected] Fax: 515-294-3261 Ph.D., Iowa State University, 1986 Email: [email protected] Research Interests: Bioinformatics, diagnostics, food Web: www.me.iastate.edu/directory/faculty/ted-heindel/ safety, nucleic acids, virology Ph.D., Purdue University, 1994 Research Description: Detection and identification of Research Interests: Bioreactors, biorenewables, veterinary and zoonotic pathogens by molecular methods. fermentation, multiphase flows Development and validation of PCR and sequencing Research Description: Transport phenomena in gas-liquid, assays. Investigation of new technologies for rapid gas-solid, and gas-liquid-solid systems, with a focus on pathogen detection. multiphase flow visualization and characterization using X-ray imaging technology, bioreactor hydrodynamics, and gas-liquid mass transfer. Hashemi, Nastaran Mechanical Engineering, Assistant Professor 2028 Black Engineering 515-294-2877 Hellmich, Richard Fax: 515-294-3261 Entomology, Associate Professor; USDA Collaborator Email: [email protected] 110 Genetics Laboratory 515-294-4509 Web: web.me.iastate.edu/hashemi/ Fax: 515-294-2265 Ph.D., Virginia Polytechnic Institute and State University, Email: [email protected] 2008 Ph.D., The Ohio State University, 1983 Research Interests: Microfluidics, optical imaging, bio- Research Interests: Integrated pest management, plant- medical engineering, biorenewables, live-cell imaging, pest interactions, transgenic plants biomaterials Research Description: Corn insect resistance to transgen- Research Description: Microfluidics, biosensors, optoflu- ic corn, insect-plant interactions related to insect resistance idics, diagnostics and therapeutics, Bio-N/MEMS (design, management, effects of transgenic corn on nontarget modeling, and fabrication) and physics of micro/nanoscale insects, such as monarch butterflies. phenomena with applications in environmental monitoring, microbial fuel cells, and portable biosensors. Helmers, Matt Agricultural and Biosystems Engineering, Assistant Profes- Hayes, Dermot J. sor; Director, Iowa Nutrient Research Center Economics, Professor 4354 Elings Hall 515-294-6717 568C Heady Hall 515-294-6185 Fax: 515-294-2552 Fax: 515-294-6336 Email: [email protected] Email: [email protected] Ph.D., University of Nebraska, 2003 Ph.D., University of California, Berkeley, 1986 Research Interests: Soil-plant interactions Research Interests: Economic implications Research Description: Water quality and water manage- Research Description: Economics of biotechnology. ment, including investigations of nutrient movement in tile-drained landscapes, water movement and management in these landscapes, and the effectiveness of vegetation Haynes, Joseph S. buffers and perennial vegetation in improving water quality. Veterinary Pathology, Professor and Chair 2718 Veterinary Medicine 515-294-0879 Fax: 515-294-5423 Henderson, Eric R. Email: [email protected] Genetics, Development and Cell Biology, Professor DVM, Ph.D., University of Missouri, 3114 Molecular Biology Building 515-294-5784 University of Minnesota, 1979, 1986 Email: [email protected] Research Interests: Animal protection from infectious Web: www.public.iastate.edu/~telomere diseases Professor, University of California, Los Angeles, 1984 Research Description: Pathogenesis of infectious disease. Research Interests: Genomics, nanotechnology, pro- Application of immunohistochemistry and in situ hybridiza- teomics, structural biology tion in study of disease in domestic animals. Research Description: Bionanoscience, DNA nanoma- chines, translational science/entrepreneurialism.

21 Faculty Profiles Hillier, Andrew Howell, Stephen H. Chemical and Biological Engineering Genetics, Development and Cell Biology Wilkinson Professor and Chair Distinguished Professor 2114 Sweeney Hall 515-294-3678 1035A Roy J. Carver Co-Laboratory 515-294-5737 Email: [email protected] Fax: 515-294-5256 Ph.D., University of Minnesota, 1995 Email: [email protected] Research Interests: Optical imaging, catalysis, imaging Web: howell-lab.gdcb.iastate.edu/ technology, nanotechnology, biosensors Ph.D., Johns Hopkins University, 1967 Research Description: Plasmonic sensing of carbohydrate Research Interests: Cell biology, developmental biology, and protein arrays using nanostructured surfaces. Biosen- genomics, molecular genetics of plants, plant cell culture, sor development using optical and surface analytical plant genetics, signal transduction, stress response in methods, including plasmonics, ellipsometry, plants, transgenic plants reflectometry, quartz crystal gravimetry, electrochemistry. Research Description: Plant stress responses. Regulation of plant gene expression. Maize and Arabidopsis genomics.

Ho Eckart, Louisa Veterinary Clinical Sciences, Assistant Professor Huang, Cheng 1832 Veterinary Medicine 515-294-4900 Electrical and Computer Engineering, Assistant Professor Email: [email protected] 2124 Coover Hall 515-294-2722 BVSc Hons MS, University of Sydney, 2004 Email: [email protected] Research Interests: Oncogenes, stem cells Ph.D., The Hong Kong University of Science and Research Description: Use of platelet rich plasma for Technology, 2014 wound healing and other disease conditions. Identifying Research Interests: Power management integrated other methods of achieving faster coagulation. circuits, wireless power transfer, fully-Integrated high-frequency switching converters, energy harvesting Research Description: Advanced Power Management IC Honzatko, Richard B. (PMIC) designs, including fully-integrated high-frequency Roy J. Carver Department of Biochemistry, Biophysics and switching power converters, wireless power transfer Molecular Biology; Professor systems, energy harvesting circuits, and other different 4206 Molecular Biology Building 515-294-7103 kinds of PMICs for various applications, such as biomedical Fax: 515-294-0453 implantable devices and wearable devices. The aim is to Email: [email protected] improve the efficiency, power density and transient per- Ph.D., Harvard University, 1982 formance, while at the same time reduce the form factor, Research Interests: Carbohydrates, enzyme mechanisms, cost and system design complexity. protein structure and function, biorenewables, antimicrobials, antimalarials Research Description: Determining structure of macro- Huang, Xiaoqiu molecules by X-ray diffraction. Enzymes that are involved Computer Science, Professor in the degradation of oligosaccharides, control of glycolysis 226 Atanasoff Hall 515-294-2432 and gluconeogenesis, biosynthesis of purine nucleotides Fax: 515-294-0258 and replication of microbial DNA. Drug development Email: [email protected] targeting of malaria and tuberculosis. Web: www.cs.iastate.edu/~xqhuang Ph.D., Penn State, 1990 Research Interests: Bioinformatics Howe, Adina Research Description: Development of algorithms and Agricultural and Biosystems Engineering software for computational problems in genome Assistant Professor sequencing and analysis. 3346 Elings Hall 515-294-0176 Fax: 515-294-6633 Email: [email protected] Web: www.germslab.org Ph.D., Iowa State University, 2008 Research Interests: Bioinformatics, metagenomics, microbial ecology, microbial genetics Research Description: Microbial interactions, ranging from engineered to natural systems. Biomarkers of environmental health and productivity, next generation sequencing, computational biology, data-driven biology.

22 Research in Biotechnology 2020 Huffman, Wallace and resilience of microbial communities following environ- Economics, Professor mental perturbations. Public health risks posed by environ- 478 Heady Hall 515-294-6359 mental biofilms. Fate and transport of antibiotic resistance Fax: 515-294-0221 genes in engineered and natural water systems. Email: [email protected] Web: www.econ.iastate.edu/faculty/huffman Ph.D., University of Chicago, 1972 Jacobs, Keri L. Research Interests: Food biotechnology, food safety Economics, Associate Professor Research Description: Economics of consumers’ valuation 469 Heady Hall 515-294-6780 of new food products made possible by the applications of Email: [email protected] biotechnology. Experimental auctions are used to assess Web: www2.econ.iastate.edu/faculty/jacobs/ the impact of information on food labels and in packaged Ph.D., North Carolina State University, 2010 information on consumers’ willingness-to-pay for low- Research Interests: Cooperative land use decisions under acrylamide biotech potato products. uncertainty Research Description: Economics of biomass production and markets and contracting for biomass supply. Hufford, Matthew B. Ecology, Evolution, and Organismal Biology Assistant Professor Janzen, Fredric J. 339A Bessey Hall 515-294-8511 Ecology, Evolution and Organismal Biology Email: [email protected] Professor Web: www.public.iastate.edu/~mhufford/HuffordLab/ 251 Bessey Hall 515-294-4230 home.html Fax: 515-294-1337 Ph.D., University of California, Davis, 2010 Email: [email protected] Research Interests: Evolutionary genomics, maize Web: www.public.iastate.edu/~fjanzen/ Research Description: The evolution and ecology of crops Ph.D., University of Chicago, 1992 and their wild relatives with a particular focus on maize and Research Interests: Animal embryos, animal genetics, the teosintes. Utilizing genomic data to study independent quantitative genetics, comparative genomics, evolutionary adaptation of maize landraces to highland environments biology, molecular ecology and to evaluate gene flow across taxa in the genus. Research Description: Integrating molecular and quantitative genetic techniques with experimental laboratory and field studies to assess the biological Hurburgh, Jr., Charles R. significance of diverse sex-determining mechanisms; the Agricultural and Biosystems Engineering, Professor impacts of environmental and genetic factors on variation 3167 NSRIC Building, 1029 N. University 515-294-8629 in early life-history traits and the current and historical Email: [email protected] genetic and demographic structure of populations. Elucidat- Ph.D., Iowa State University, 1981 ing adaptive processes and solving conservation concerns. Research Interests: Food preservation and quality, food processing, product certification and traceability, grain and biomass analytics Jarboe, Laura Research Description: Food safety and quality manage- Chemical and Biological Engineering, Assistant Professor ment systems, grain processing, certification and traceabil- 4134 Biorenewables Research Laboratory 515-294-2319 ity, analytics and quality control, spectroscopic methods, Fax: 515-294-2689 chemometrics. Email: [email protected] Web: www.cbe.iastate.edu/the-department/ facultystaff/?user_page=ljarboe Ikuma, Kaoru Ph.D., University of California, Los Angeles, 2006 Civil, Construction and Environmental Engineering Research Interests: Biorenewables, metabolic engi- Assistant Professor neering, microbial genetics, microbial virulence, stress 328 Town Engineering 515-294-7660 response in microorganisms, systems biology, antibiotic Email: [email protected] resistance Ph.D., Duke University, 2011 Research Description: Engineering and evolution of Research Interests: Antibiotic resistance, bioremediation bacterial biocatalysts for tolerance of inhibitory products of environmental pollutants, metagenomics, microbial or substrate contaminants. Soil attachment and antibiotic ecology, stress response in microorganisms resistance by agricultural E. coli isolates. Research Description: Interactions between environmental biofilms and contaminants with special focus on contaminant transport and biodegradation. Stress response

23 Faculty Profiles Jelesijevic, Tomislav Jiang, Shan Veterinary Pathology; Assistant Professor Materials Science and Engineering, Assistant Professor 2720 Veterinary Medicine 515-294-0614 Hoover 2220BD 515-294-1445 Fax: 515-294-7730 Email: [email protected] Email: [email protected] Web: sjiang1.public.iastate.edu PhD, University of Georgia-Athens, 2011 Ph.D., University of Illinois at Urbana-Champaign, 2009 Research Interests: Immunomodulation in infectious dis- Research Interests: Biomaterials, nanomaterial, gene eases and/or neoplasia. Canine and Feline mammary gland transfection, biomedical engineering, drug and gene tumors delivery, cancer, cancer genetics, drug detection Research Description: Immunomodulation of immune Research Description: Gene and drug delivery through responses mediated by myeloid derived suppressor cells nanoparticles. (MDSCs) in infectious diseases and tumors.

Jiles, David C. Jenks, William Electrical and Computer Engineering Chemistry, Professor and Chair Distinguished Professor and Chair 1605 Gilman Hall 515-294-7871 2215 Coover 515-294-8353 Email: [email protected] Fax: 515-294-3637 Ph.D., Columbia University, 1991 Email: [email protected] Research Interests: Enzyme mechanisms, photosynthesis Web: www.engineering.iastate.edu/directory/?user_ and photorespiration page=dcjiles Research Description: Study of photochemistry of DSc., University of Birmingham, United Kingdom, 1990 aromatic sulfoxides, active oxygen species and oxidations. Research Interests: Biomedical engineering, brain Molecular recognition techniques in photochemistry and research, transcranial magnetic stimulation, non-invasive electron transfer. Photocatalytic degradation of organic brain stimulation, non-invasive treatment of brain disorders waste products. Research Description: Application of non-invasive magnetic methods, specifically transcranial magnetic stimulation (TMS), for stimulation of the brain for diagnostic and Jernigan, Robert therapeutic purposes and as a replacement for surgery. Roy J. Carver Department of Biochemistry, Biophysics and Development of hardware systems for deep brain stimula- Molecular Biology; Professor tion for treatment of diseases and brain disorders such as 112 Office and Labs Building 515-294-3833 traumatic brain injury, concussion, depression, Parkinson’s Fax: 515-294-3841 disease, ischemic stroke and vascular dementia. Non- Email: [email protected] invasive stimulation of the brain for identification of func- Web: www.public.iastate.edu/~jernigan/ tionality of different regions. Use of magnetic methods for Ph.D., Stanford University, 1968 stimulation of the peripheral nervous system. Application Research Interests: Bioinformatics, biomedical engineer- of computer modeling methods to simulation of magnetic ing, comparative genomics, computational biology, DNA fields in the brain. structure and function, genomics, molecular evolution, molecular pharmacology, nucleic acids, biomaterials, protein engineering, protein structure and function, Johannes, Chad proteomics, RNA structure and function, structural biology Veterinary Clinical Sciences, Assistant Professor Research Description: Computational studies on the 1569 LVMC 515-291-5216 structures of cellular assemblies, interacting proteins and Email: [email protected] proteins interacting with nucleic acids, proteins, and small DVM, DACVIM (SAIM, Oncology), Kansas State University, molecules and their interactions. Functional mechanisms of 1997 structures. Protein datamining, threading and conformation Research Interests: Cancer, immunobiology, monoclonal generation. Protein and docked-model evaluations. Free antibodies, oncogenes, vaccines energies, entropies and interaction energies. Applications Research Description: Translational oncology research include gene annotations, structure interaction predictions, with a focus in small molecule inhibitors and immuno- developing molecular models and selecting new drugs. oncology platforms (monoclonal antibodies, therapeutic vaccines). Strong interest in drug development for dog and cat oncology therapeutics.

24 Research in Biotechnology 2020 Johansen, Jorgen Research Description: Self-assembly is a process by Roy J. Carver Department of Biochemistry, Biophysics and which micro- or nanoscopic building blocks, such Molecular Biology; Professor as small particles, polymers or proteins are used to fabri- 3156 Molecular Biology Building 515-294-2358 cate new types of materials for energy storage (e.g., solar Fax: 515-294-4858 cells) or drug delivery (e.g., encapsulation of a pharmaceuti- Email: [email protected] cal). The processes for fabrication are often slow and lim- Web: www.bbmb.iastate.edu/people/jorgen-johansen ited by the rate of diffusion. In order to speed up the self- Ph.D., University of Copenhagen, 1984 assembly process, the Juárez lab uses externally directed Research Interests: Cell biology, cytochemistry, develop- fields (e.g., electric, magnetic, acoustic) to increase the mental biology, DNA structure and function, gene expres- rate of assembly. Our aim is to adapt the use of external sion, protein structure and function fields as a process to improve the additive manufacturing Research Description: Nuclear division, oncogenesis, cell- (3D printing) of composite materials. Tuning the composi- cycle control and signal transduction. Chromatin structure tional structure of composites can lead to improvements and function. in the functional quality of these composites, including enhancements to mechanical stability for soft robotics or sensitivity to local chemistry for biosensing. Johansen, Kristen Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology; Roy J. Carver Professor and Chair Jurenka, Russell A. 1210 Molecular Biology Building 515-294-0528 Entomology, Professor Fax: 515-294-7629 434 Science II 515-294-1485 Email: [email protected] Email: [email protected] Web: www.bbmb.iastate.edu/people/kristen-johansen Web: www.ent.iastate.edu/people/russell-jurenka Ph.D., Yale University, 1989 Ph.D., University of Nevada, Reno, 1987 Research Interests: Animal cell culture, biochemical Research Interests: Animal physiology, insect toxicology, engineering, gene expression, monoclonal antibodies, cell lipids, natural products, neuroscience biology, genomics, live cell imaging, molecular genetics of Research Description: Biochemical mode of action of animals, protein structure and function, signal transduction insect peptide hormones, including site of synthesis and Research Description: Regulation of nuclear division, on- release, signal transduction and receptor identification. cogenesis, cell-cycle control and signal transduction events Enzymes involved in insect lipid metabolism, including in cell differentiation. Chromatin structure and function. biosynthesis of pheromones, polyunsaturated fatty acids and prostaglandins.

Jones, Douglas E. Veterinary Pathology, Associate Professor Kadelka, Claus 2750 Veterinary Medicine 515-294-4682 Mathematics; Assistant Professor Fax: 515-294-5423 444 Carver Hall 515 294 7579 Email: [email protected] Email: [email protected] DVM, University of Pennsylvania, 1989 Web: faculty.sites.iastate.edu/ckadelka/ Research Interests: Animal protection from infectious dis- PhD, Virginia Tech, 2015 eases, cell biology, computational biology, immunobiology, Research Interests: Mathematical biology, mathematical microbial virulence, vaccines modeling, bioinformatics, nonlinear dynamics, network Research Description: Identification of the cellular and science, biomedical data science, molecular epidemiology, molecular factors that influence the host-parasite relation- HIV immunology, gene regulation ship. Investigating the mechanisms by which the intracellu- Research Description: Unraveling the mechanisms under- lar parasite L. amazonensis inhibits the development of an lying the robustness of gene regulatory networks and HIV effective cell-mediated immune response. Development of antibody induction/vaccine development. an immune-feedback regulated vaccine device. Kanthasamy, Anumantha Juarez, Jaime J. Biomedical Sciences, Professor and W. Eugene and Linda Mechanical Engineering, Assistant Professor Lloyd Endowed Professor 2020 Black Engineering 515-294-3298 2062 Veterinary Medicine 515-294-2516 Email: [email protected] Fax: 515-294-2315 Ph.D., Johns Hopkins University, 2011 Email: [email protected] Research Interests: Biomaterials, biomedical engineering, Web: vetmed.iastate.edu/users/akanthas microfluidics, nanotechnology, video microscopy Ph.D., University of Madras, 1990

25 Faculty Profiles Research Interests: Immunobiology, proteomics, stem Research Description: Tools for the detection and treat- cells, toxicology, transgenic animals ment of diseases in swine populations. Analgesic com- Research Description: Cellular/molecular mechanisms for pounds and technology for the detection and treatment of pathogenesis of Parkinson’s disease and related neurologi- clinical signs of pain in swine. cal disorders. Characterization of mechanism-based therapeutic strategies for these disorders. Cellular and molecular changes in dopaminergic neurons following exposure to Keating, Aileen F. neurotoxic pesticides and other chemicals that are risk Animal Science, Associate Professor factors for Parkinson’s disease. Pathogenic mechanism 2356J Kildee Hall 515-294-3849 underlying prion diseases. Fax: 515-294-4471 Email: [email protected] Web: www.ans.iastate.edu/people/aileen-keating Kanthasamy, Arthi Ph.D., National University of Ireland, Galway, 2003 Biomedical Sciences, Assistant Professor Research Interests: Animal physiology, animal reproduc- 2024 Veterinary Medicine 515-294-7238 tive efficiency, toxicology, signal transduction, human Fax: 515-294-2315 physiology, gene expression Email: [email protected] Research Description: Investigation of how xenobiot- Web: vetmed.iastate.edu/users/arthik ics cause disruption to ovarian function. Specifically, how Ph.D., Purdue University, 2001 chemicals cause destruction of ovarian follicles leading to Research Interests: Gene expression, cell biology, animal infertility and early menopause, and improvement of cell culture, neuroscience, signal transduction, toxicology, fertility in humans and production animals. transgenic animals Research Description: Cellular and molecular mechanisms underlying stroke, Parkinson’s disease and related neuro- Kelley, Dior logical disorders. Developing gene therapy technologies Genetics, Development and Cell Biology aimed at limiting ischemia-induced brain damage. Evalua- Assistant Professor tion of the signal transduction cascades underlying PD- 3011 ATRB 294-2438 induced neuronal damage with particular emphasis on the Email: [email protected] role of autophagy in dopaminergic neuronal degeneration. Web: www.gdcb.iastate.edu/kelleylab/ Ph.D., UC Davis, 2009 Research Interests: Plant biology, hormone signaling, plant Kanwar, Rameshwar S. development, omics, networks. Agricultural and Biosystems Engineering Research Description: Hormone regulated plant develop- C. F. Curtiss Distinguished Professor ment in Arabidopsis and maize. 4358 Elings Hall 515-294-1434 Fax: 515-294-6633 Email: [email protected] Kerr, Brian J. Ph.D., Iowa State University, 1981 Animal Science, Associate Professor; USDA, Collaborator Research Interests: Bioremediation of environmental 2165 National Swine Research and Information Center pollutants, photosynthesis and photorespiration, soil-plant 515-294-0224 interactions, stress response in plants, waste management Fax: 515-294-1209 Research Description: Chemical and bacterial transport in Email: [email protected] the environment, quality of groundwater and surface water, Ph.D., University of Illinois, 1988 manure management and its effects on water quality, ex- Research Interests: Animal nutrition, bioavailability of cessive water stress on plants and irrigation and drainage. nutrients, carbohydrates, vitamins and minerals, waste management Research Description: Amino acid needs for optimal pig Karriker, Locke performance and carcass lean deposition, nitrogen and Veterinary Diagnostic and Production Animal Medicine amino acid utilization, crystalline- and protein-bound amino Assistant Professor acid absorption, mineral balance, impact of carbohydrate 2227 Lloyd Veterinary Medical Center 515-294-2283 source on gastrointestinal and manure microbial ecology, Fax: 515-294-1072 impact of nutritional programs on malodorous compound Email: [email protected] production. DVM, Diplomate, Mississippi State College of Veterinary Medicine, American College of Veterinary Preventive Medicine, 1999, 2006 Research Interests: Animal protection from infectious diseases, epidemiology, biosecurity, antimicrobials, pharmacokinetics 26 Research in Biotechnology 2020 Kimber, Michael J. Khokhar, Ashfaq Biomedical Sciences, Interim Department Chair Electrical and Computer Engineering, Associate Professor Professor and Palmer Department Chair 2008 Veterinary Medicine 515-294-4415 2211 Coover Hall 515-294-2664 Fax: 515-294-2315 Email: [email protected] Email: [email protected] Ph.D., University of Southern California, 1993 Ph.D., Queen’s University, Belfast, 2001 Research Interests: Big Data, bioinformatics, biomedical Research Interests: Animal physiology, gene expression, engineering, biosensors, electronic health records, evolu- immunobiology, infectious diseases, neuroscience, RNA tionary biology, genome mapping, genomics, imaging tech- structure and function, host-parasite interactions. nology, molecular ecology, proteomics, STEM education Research Description: Biology of parasitic nematodes, in Research Description: Investigation of high-performance particular, filarial nematodes that are the etiological agents computing solutions for Big Data proteomics. Investiga- of human lymphatic filariasis. Study of the neurobiology tion of efficient techniques for wild population evolutionary of these parasites in an effort to better understand how biology problems such as sibling and multilevel inheritance. they coordinate neuromuscular function and perceive their Application of machine learning techniques in biomedical environment. Focus on parasite small RNAs as a mecha- imaging diagnosis. Development of IoT systems for nism for regulating gene expression and parasite-derived biosensor-based application. extracellular vesicles as manipulators of the host-parasite interface.

Kim, A. Ram Aerospace Engineering; Assistant Professor Knapp, Allen 2239 Howe Hall 515-294-8850 Agronomy, Professor Email: [email protected] 1557 Agronomy Hall 515-294-9830 Ph.D., University of Kansas, 2018 Fax: 515-294-5506 Research Interests: Autonomous system, bio-inspired Email: [email protected] navigation, guidance and control algorithms Web: www.agron.iastate.edu/people/allen-knapp Research Description: Swarm of unmanned aerial sys- Ph.D., Washington State University, 1981 tems, collaboration of multi-agent unmanned systems. Research Interests: Stress response in plants, seed physiology, plant physiology Research Description: Investigation of low temperature stress response of maize during germination and heterotro- Kim, Jaeyoun phic seedling growth. Characterization of physiological and Electrical and Computer Engineering, Associate Professor genetic changes in closed maize populations as a result 2216 Coover Hall 294-4214 of selection. Mapping of QTL associated with corn seed Email: [email protected] quality. Ellucidation of the mechanisms of seed and plant Web: www.plasmonics.net dormancy. Investigation of seed quality in maize Ph.D., University of Michigan, 2003 recombinant inbred lines. Research Interests: Biomedical engineering, microfluidics, nanotechnology, optical imaging Research Description: Unconventional microtechnol- ogy for biomedical sensors and actuators. Plasmonic and Kohut, Marian L. optical sensing of molecules. Biomimetic and bioinspired Kinesiology, Professor engineering. 235 Forker Building 515-294-8364 Fax: 515-294-8740 Email: [email protected] Ph.D., University of South Carolina, 1996 Kim, Jinoh Research Interests: Antiviral agents, immunobiology, Biomedical Sciences/Associate Professor senescence, human nutrition and health, vaccines Vet Med 2086 515-294-3401 Research Description: Investigation of age-associated Email: [email protected] alterations in immune response to vaccines or viral infec- Web: kimlab.research.cvm.iastate.edu/ tion. Role of innate host defense in modifying adaptive Associate Professor, University of Connecticut, 2000 immunity to influenza. Stress-induced immunomodulation, Research Interests: Intracellular protein trafficking, neuroendocrine-immune interaction and effect of exercise ER stress and botanicals on immune system. Research Description: My laboratory studies COPII-dependent ER export and human diseases caused by defec- tive ER export. We also study diseases contributed by aberrant ER stress responses.

27 Faculty Profiles Koltes, James Kreuder, Amanda Animal Science, Assistant Professor Veterinary Microbiology and Preventive Medicine 239A Kildee Hall 515-294-8274 Assistant Professor Email: [email protected] 2124 Veterinary Medicine 515-294-2174 Web: www.ans.iastate.edu/people/james-koltes Email: [email protected] Ph.D., Iowa State University, 2007 Ph.D, DVM, Iowa State University, 2008; 2016 Research Interests: Bioinformatics, genomics, phenomics Research Interests: Molecular pathogenesis of zoonotic Research Description: Applications of genetics, genomics bacterial diseases, antimicrobial resistance and automated data collection to enhance animal health and efficiency. Bioinformatics research focuses on supporting these goals, through the analysis of sequence Kudva, Indira T. data to better understand gene expression, regulation and Food Safety and Enteric Pathogens Research Unit, National variant function as resources for the animal genomics com- Animal Disease Center, Lead Research Microbiologist; munity. Veterinary Microbiology and Preventive Medicine, Adjunct Assistant Professor NADC/USDA, Building. 20/Rm 1121, 1920 N. Dayton Ave. Koziel, Jacek 515-337-7376 Agricultural and Biosystems Engineering Email: [email protected] Associate Professor Web: www.ars.usda.gov/midwest-area/ames/nadc/people/ 4350 Elings Hall 515-294-4206 indira-kudva/ Fax: 515-294-4250 B.Sc., M.Sc., Ph.D.; Stella Maris College, India; Kasturba Email: [email protected] Medical College, India; University of Idaho; 1984, 1987, Web: www.biotech.iastate.edu/biotechnology-service-facili- 1997 ties/atmospheric-air-quality-laboratory/ Research Interests: Animal cell culture, animal physiol- Ph.D., University of Texas, 1998 ogy, animal protection from infectious diseases, antibiotic Research Interests: Odor, biochemical engineering, bio- resistance, antibody diversity, antibody structure-function, logical compound separation and detection, bioreactors, antimicrobials, B cell immunity, bacterial microcomponents, biorenewables, bioremediation of environmental pollutants, bioinformatics, cell adhesion, comparative genomics, biosecurity, drug detection, grain and biomass analysis, cytochemistry, DNA structure and function, diagnostics, metabolomics, nanotechnology, natural products, plant- epidemiology, food safety, functional genomics, gene pest interactions, product certification and traceability, expression, genomics, immunobiology, infectious diseases, toxicology, waste management metabolomics, metagenomics, microbial ecology, micro- Research Description: Air quality engineering and live- bial genetics, microbial identification, microbial virulence, stock odor control. Gas, dust and odor emissions moni- microfluidics, molecular epidemiology, molecular genetics toring and abatement. Development and testing of odor of microorganisms, molecular mechanisms, monoclonal control technologies. Simultaneous chemical and sensory antibodies, nanotechnology, nucleic acids, proteomics, risk analysis. Development of novel analytical methods for assessment, RNA structure and function, STEM education, chemical and olfactory analysis of air, water, wastewater, stress response in animals, stress response in microorgan- manure, process gas, biorenewables, volatiles emitted isms, systems biology, tissue engineering, transcriptome by plants, crops, and insects. Metabolomics. Solid phase analysis, transgenic animals, transposable elements, microextraction and multidimensional gas chromatography- vaccines mass spectrometry. The fate of trace volatile organic com- Research Description: Develop preharvest strategies to pounds in the environment. control STEC in cattle in order to minimize ‘farm to fork’ contamination of food using global (proteomics, metabolomics, microbiome analysis) and targeted (genetic, Kraus, George A. histological, cell and in vitro organ culture, animal study) Chemistry, University Professor technologies to analyze STEC colonization dynamics and 2759 Gilman Hall 515-294-7794 identify key targets for therapeutic and diagnostic use. Fax: 515-294-0105 Projects include STEC interactions with the bovine gastro- Email: [email protected] intestinal cells, especially those at the recto-anal junction Ph.D., Columbia University, 1976 (RAJ); STEC factors that promote its survival in the bovine Research Interests: Biorenewables, virology, nanotechnol- rumen and persistence at the RAJ; adherence mechanisms ogy, natural products deployed by STEC in strain and host-dependent manner; Research Description: Synthesis of novel antiviral agents. development of rational vaccines and vaccine-alternatives Applications of bioprivileged molecules. Development of that target STEC in cattle; and development of diagnostic industrial uses for renewable resources. assays to study STEC adherence and to identify STEC- colonized cattle.

28 Research in Biotechnology 2020 Lamkey, Kendall R. Research Description: Identifying mechanisms of Agronomy, Chair and Professor microvascular dysfunction in response to aging, stress, or 2101 Agronomy Hall 515-294-7636 other pathological conditions. Human skin is used as an in Fax: 515-294-3163 vivo indicative of pathogenic changes and interventions to Email: [email protected] improve microvascular function to reduce disease Web: www.agron.iastate.edu/people/kendall-lamkey progression. Ph.D., Iowa State University, 1985 Research Interests: Plant breeding, quantitative genetics Research Description: Use of plant breeding to increase Lathrop, James the biomass production of corn. Focus is on both grain and Computer Science, Senior Lecturer stover production. Plant breeding research is being con- B4 Atanasoff 294-5817 ducted to identify genetic characteristics of corn for growth Email: [email protected] and production in living mulches. Web: www.cs.iastate.edu/~jil Ph.D., Iowa State University, 1997 Research Interests: Nanotechnology, STEM education Lamont, Susan J. Research Description: Molecular programming and Animal Science, Distinguished Professor verification. 2255 Kildee Hall 515-294-4100 Fax: 515-294-2401 Email: [email protected] Lauter, Nick Web: www.ans.iastate.edu/people/susan-lamont Plant Pathology and Microbiology, Assistant Professor Ph.D., University of Illinois Medical Center, 1980 415 Bessey Hall 515-294-8260 Research Interests: Animal breeding, animal genetics, Fax: 515-294-9420 animal protection from infectious diseases, gene Email: [email protected] expression, genome mapping, genomics, immunobiology, Ph.D., University of Minnesota, 2001 molecular genetics of animals, systems biology Research Interests: Molecular genetics of plants, gene Research Description: Identification of genetic markers, expression, plant-pest interactions, quantitative genetics, genes and pathways for resistance to pathogens and to metabolomics, bioinformatics heat stress. Defining the genetic architecture underlying Research Description: Discovering and characterizing complex biological traits. Isolation and characterization of agronomically-important genes in cereal crops. Understand- genes and proteins regulating immunoresponsiveness, ing the host regulatory mechanism underlying complex fitness and growth in poultry. Production of healthier and transcriptional responses to inoculation and infection by more efficient poultry through genetic selection. Genomic powdery mildew and stem rust. Life history traits and their selection. relevance to disease resistance and defense capability in corn. Definition of the extent to which basal defense genes are expressed only after the onset of adult shoot identity. Lamsal, Buddhi Food Science and Human Nutrition, Associate Professor 2312 Food Sciences Building 515-294-8681 Lavrov, Dennis Fax: 515-294-8181 Ecology, Evolution and Organismal Biology Email: [email protected] Associate Professor Ph.D., University of Wisconsin, 2004 343A Bessey Hall 515-294-9091 Research Interests: Fermentation, food processing, food Fax: 515-294-1337 biotechnology, biomaterials, biorenewables, plant protein Email: [email protected] and Oil Utilization Web: lavrovlab.github.io/ Research Description: Value-added utilization of agricul- Ph.D., University of Michigan, 2001 tural crops, fermentation and bioprocessing, pre- and pro- Research Interests: Animal genetics, bioinformatics, biotics, biomass utilization for bioenergy and biomaterials, comparative genomics, molecular evolution, organelle non-food use of crops and their components. genetics, RNA structure and function Research Description: Our main research interests are animal evolution, molecular phylogenetics, and mitochon- Lang, James, A. drial biology. Our favorite animals are marine non-bilaterian Kinesiology, Assistant Professor taxa, including Porifera (sponges), Cnidaria (corals, jel- 0103R Forker Building 5152944027 lyfishes), and Ctenophora (comb jellies). From a phyloge- Email: [email protected] netic perspective, each of these groups forms at least an Ph.D., Penn State University, 2010 equal-status taxon to all bilaterian animal (from flatworms Research Interests: Microvascular skin blood flow, to humans) and thus should harbor a large portion of thermoregulation animal genomic diversity that we are only now beginning to explore. 29 Faculty Profiles Lawrence-Dill, Carolyn Lee, Michael Agronomy; Genetics, Development and Cell Biology Agronomy, Professor Associate Professor 1553 Agronomy Hall 515-294-7951 0035B Roy J. Carver Co-Laboratory 515-294-4294 Fax: 515-294-3052 Fax: 515-294-5256 Email: [email protected] Email: [email protected] Ph.D., University of Minnesota, 1986 Web: dill-picl.org/ Research Interests: Molecular genetics of plants, plant Ph.D., University of Georgia, 2003 breeding, plant genetics Research Interests: Bioinformatics, phenomics, compara- Research Description: DNA markers, genetic mapping, tive genomics, evolutionary biology, functional genomics, genome organization, genetics and breeding of maize, genomics, molecular genetics of plants, plant genetics, other grasses and plant model systems. Identification of systems biology genes for biotic and abiotic stress and grain quality. Genetic Research Description: We develop computational sys- diversity. tems and tools that enable researchers to leverage plant genetics and genomics information to better understand basic biology and effect crop improvement. Group mem- Lee, Young-Jin bers are specifically interested in functional prediction for Chemistry, Associate Professor genes, predictive phenomics, and how gene and chromo- 35A Roy J. Carver Co-Lab 515-294-1235 some architecture regulate cellular processes. Although Fax: 515-294-0105 work by group members is not specifically limited to maize, Email: [email protected] it is by far our favorite model system. Web: yjlee.public.iastate.edu/ Ph.D., Seoul National University, 1997 Research Interests: Mass spectrometry, chemical imag- Leandro, Leonor ing, metabolomics, proteomics, pyrolysis GC-MS, bio-oil Plant Pathology and Microbiology, Associate Professor analysis 325 Bessey 515-294-8855 Research Description: Development and application of Fax: 515-294-9420 mass spectrometric techniques for biological or engineer- Email: [email protected] ing applications. Mass spectrometric imaging of plant Ph.D., Iowa State University, 2002 metabolites. High resolution mass spectrometric analysis Research Interests: Epidemiology, integrated pest man- of thermochemically-converted bio-oils. High-throughput agement, molecular genetics of microorganisms, soil-plant metabolomics. interactions, plant-pest Interactions, plant breeding Research Description: Host-pathogen interactions in soybean fungal pathogens. Characterizing genetic diversity of Lence, Sergio Fusarium virguliforme populations. Detection and quantifi- Economics; Professor and Marlin Cole Chair of cation of Fusarium in soil and roots using RT-PCR. International Agricultural Economics Phenotyping resistance of soybean varieties to sudden 368E Heady Hall 515-294-8960 death syndrome and soybean rust. Fax: 515-294-6644 Email: [email protected] Web: www.econ.iastate.edu/people/sergio-lence Lee, Duck-chul Ph.D., Iowa State University, 1991 Kinesiology, Associate Professor Research Interests: Economic implications, product 103H Forker Building 515-294-8042 certification and traceability, transgenic plants Fax: 515-294-8740 Research Description: Market and welfare effects of Email: [email protected] the introduction of genetically-modified products. Welfare Ph.D., Seoul National University, 2007 impact of different intellectual property right regimes on Research Interests: Physical activity epidemiology private-sector seed research. Research Description: Physical activity epidemiology, especially with regard to the independent and combined effects of aerobic and resistance exercise on various health LeVine, Dana outcomes in adults. The associations of physical activity, Veterinary Clinical Sciences; cardiorespiratory fitness, muscular strength, dietary pattern Assistant Professor of Small Animal Internal Medicine and alcohol consumption with various health outcomes, 2516 Lloyd Veterinary Medical Center 515-294-4900 including hypertension, type 2 diabetes, obesity, falling, Email: [email protected] depression, and cardiovascular disease morbidity and DVM, Ph.D., Cornell University, North Carolina State mortality. University, 2004, 2013 Research Interests: Hematology, thrombosis, immune thrombocytopenia, platelets, NETs

30 Research in Biotechnology 2020 Li, Ganwu Linhares, Daniel Veterinary Diagnostic and Production Animal Medicine Veterinary Diagnostic and Production Animal Medicine Assistant Professor Assistant Professor 201 VMRI Building 4, 515-294-3358 2221 Lloyd Veterinary Medical Center 515-294-9358 Email: [email protected] Email: [email protected] Ph.D., Berlin Free University, Berlin, Germany; 2005 DVM, MBA, Ph.D., Universidade Federal de Goias, Funda- Research Interests: Functional genomics, microbial cao Getulio Vargas, University of Minnesota, 2003, 2007, identification, microbial virulence, molecular virology 2013 Research Description: Characterization and identification Research Interests: Biosecurity, economic implications, of animal pathogens by using next-generation sequencing epidemiology, infectious diseases, risk assessment technology. Investigation of microbial virulence, with spe- Research Description: Applied research on swine health cial emphasis on the virulence mechanisms of and productivity challenges, including evaluation of strate- extraintestinal pathogenic E. coli. gies to reduce frequency of new pathogen introduction, and to increase success rate and effectiveness of pathogen control and elimination programs. Ongoing projects Li, Qi include applied research with PRRSV, Mycoplasma hyo- Computer Science; Assistant Professor pneumoniae, Influenza A virus and Senecavirus A (former 0102 Atanasoff 294-0081 Seneca Valley) virus. Email: [email protected] Web: sites.google.com/iastate.edu/qili/ Ph.D, State University of New York - Buffalo Lippolis, Katy Research Interests: Data mining, text mining Animal Science, Assistant Professor Research Description: Conducting information extraction 313D Kildee Hall 5152943020 from biomedical literature to facilitate the search and Email: [email protected] analysis of scientific publications. Ph.D., Oregon State University, 2018 Research Interests: Beef cattle, nutrition, weaning, immunity, health, morbidity, bovine respiratory disease Li, Qing Industrial and Manufacturing Systems Engineering Assistant Professor Liu, Peng 3031 Black Engineering 515-294-4867 Statistics, Associate Professor Email: [email protected] 2117 Snedecor Hall 515-294-7806 Web: www.imse.iastate.edu/qing-li/ Fax: 515-294-4040 Ph.D., Virginia Tech, 2015 Email: [email protected] Research Interests: Bayesian hierarchical modeling, Ph.D., Cornell University, 2006 recurrent-event change-point detection, Poisson process, Research Interests: Analysis of Next-Generation sequenc- clustering, signal processing ing data, bioinformatics, biostatistics, gene expression, Research Description: Statistical analysis. microbiomes, metagenomics, quantitative genetics Research Description: Statistical methods for the analysis of high-dimensional genomic data such as next-generation Licht, Mark A. sequencing data and microbiome data. Agronomy, Assistant Professor 2104M Agronomy Hall, 716 Farm House Lane 515-294-0877 Lonergan, Elisabeth Email: [email protected] Animal Science, Professor Web: www.agron.iastate.edu/people/mark-licht 215A Meat Laboratory 515-294-9125 Ph.D., Iowa State University, 2015 Fax: 515-294-9143 Research Interests: Big Data, plant physiology, plant-pest Email: [email protected] interactions, soil-plant interactions, stress response in Web: www.ans.iastate.edu/people/elisabeth-huff-lonergan plants, transgenic plants Ph.D., Iowa State University, 1995 Research Description: Extension, research and teach- Research Interests: Food biotechnology, food preservation ing program focused on how to holistically manage Iowa and quality, muscle biology, proteomics cropping systems to achieve productivity, profitability and Research Description: Identification of molecular environmental goals. Varied aspects of soybean, corn and mechanisms of muscle growth and postmortem conver- cover crop management, as well as agronomic implications sion of muscle to meat. Application of modern proteomics of precision technologies. techniques to understand how to improve the efficient growth of lean muscle.

31 Faculty Profiles Lonergan, Steven Lu, Chaoqun Animal Science, Professor Ecology, Evolution, and Organismal Biology 215B Meat Laboratory 515-294-9126 Assistant Professor Fax: 515-294-9193 143 Bessey Hall 515-294-7443 Email: [email protected] Email: [email protected] Web: www.ans.iastate.edu/people/steven-lonergan Web: clu.public.iastate.edu/ Ph.D., University of Nebraska, Lincoln, 1995 Ph.D., Chinese Academy of Science, and Auburn Univer- Research Interests: Meat science and technology sity, 2009 Research Description: Improving muscle growth and Research Interests: Soil-plant interactions meat quality in livestock. Protein turnover in muscle and Research Description: Modeling representation and the regulation of proteolysis in muscle catalyzed by calpain assessment of soil-plant-atmosphere interactions, including proteinases, using chromatography, SDS-PAGE, 2D-PAGE, carbon, nitrogen and water cycling, greenhouse gas 2D-DIGE and other proteomic applications. emissions, nutrient movement from land to aquatic systems within the context of climate change, atmospheric composition change, land use and management practices Looft, Torey at scales ranging from site to watershed, region, nation, Veterinary Microbiology and Preventive Medicine and globe. Collaborator/Assistant Professor Building 20, National Animal Disease Center 515-337-7140 Email: [email protected] Lu, Meng Ph.D., Iowa State University, 2012 Electrical and Computer Engineering Research Interests: Bioinformatics, Gene Expression, Assistant Professor Metagenomics, Microbial Ecology, Molecular Ecology 2128 Coover Research Description: Using metatranscriptomics, Email: [email protected] metagenomics, and genomic techniques to study bacterial Ph.D., University of Illinois at Urbana Champaign, 2008 gene expression and spatial distribution within the intesti- Research Interests: Optical biosensors, point-of-care nal tract of poultry. Identifying external (management, diet) tests, Lab-on-a-chip and internal (intestinal microbes) factors. Research Description: Design, prototype, and test sensors and instruments for applications, such as antimicrobial resistance, cancer diagnosis, and exosomes. Loving, Crystal L. Veterinary Microbiology and Preventive Medicine, USDA-ARS-NADC, Adjunct Faculty Lübberstedt, Thomas Science University of Montpellier 515-337-7364 Agronomy, Professor, K. J. Frey Chair Email: [email protected] 1204 Agronomy Hall 515-294-5356 Web: www.ars.usda.gov/people-locations/person/?person- Fax: 515-294-3163 id=44824 Email: [email protected] Ph.D., Iowa State University, 2006 Web: www.plantbreeding.iastate.edu/DHF/DHF.htm Research Interests: Animal genetics, animal nutrition, ani- Ph.D., University of Munich, 1993 mal physiology, animal protection from infectious diseases, Research Interests: Biorenewables, comparative genom- antibiotic resistance, antimicrobials, B cell immunity, com- ics, gene expression, genome mapping, genomics, mo- putational biology, food safety, functional genomics, gene lecular genetics of plants, plant breeding, plant genetics, expression, genomics, immunobiology, infectious plant-pest interactions, plant physiology, quantitative genet- diseases, metabolomics, microbial ecology, microbial ics, stress response in plants, doubled haploids genetics, microbial virulence, STEM education, stress Research Description: Application and development of response in animals, transcriptome analysis, vaccines tools and methods provided by genome analysis (especially Research Description: Identifying critical interactions be- DNA markers) to understand composition of complex traits tween commensal intestinal microbiota and the developing and phenomena, to determine and exploit genetic diversity immune system that contribute to a balanced and healthy in elite and exotic germplasm and apply this knowlege pig. Development of intervention strategies to limit in-feed to plant breeding. Forage quality, vernalization response, antibiotic use and colonization with foodborn pathogens. resistance, and heterosis in ryegrass and maize. Develop- ment of gene-derived, functional markers and their implementation in plant breeding, as well as in breeding bioenergy crops.

32 Research in Biotechnology 2020 Lutz, Jack H. Research Interests: Cancer, human nutrition and health, Computer Science, Professor natural products 226 Atanasoff 515-294-9941 Research Description: Dietary compounds’ role in the Fax: 515-294-0258 etiology and regulation of colon cancer and inflammatory Email: [email protected] bowel disease. Cellular changes induced in the carcino- Web: www.cs.iastate.edu/~lutz genic process and mediation of these events by dietary Ph.D., California Institute of Technology, 1987 compounds. Cellular biomarkers of cancer that are influ- Research Interests: Nanotechnology, nucleic acids enced by dietary compounds using proteomic and meta- Research Description: Molecular programming and DNA bolic approaches. nanotechnology. Universality, robustness, self-assembly, information flow, modularity, programming methods, randomness, fractal geometry, verification, and safety in MacIntosh, Gustavo chemical reaction networks, DNA strand displacement Roy J. Carver Department of Biochemistry, Biophysics and systems, DNA tile assembly, DNA origami, and molecular Molecular Biology; Associate Professor robotics. 4108 Molecular Biology Building 515-294-2627 Email: [email protected] Ph.D., Universidad de Buenos Aires, Argentina, 1997 Lutz, Robyn R. Research Interests: Cell biology, gene expression, Computer Science, Professor molecular genetics of plants, nucleic acids, plant genetics, 226 Atanasoff 515-294-3654 plant-pest interactions, plant physiology, proteomics, stress Fax: 515-294-0258 response in plants Email: [email protected] Research Description: Gene expression and metabolic Web: www.cs.iastate.edu/~rlutz changes during plant defense responses to pests, Ph.D., University of Kansas, 1980 functional genomics of plant nucleases. Research Interests: Nanotechnology, nucleic acids Research Description: Molecular programming and self-assembly. Software engineering, software safety, Maier, Dirk requirements engineering, product lines, formal methods Agricultural and Biosystems Engineering, Professor for specification and verification, fault analysis and fault 3325 Elings Hall 515-294-0140 tolerance for DNA origami and molecular robotics. Email: [email protected] Web: www.abe.iastate.edu Ph.D., Michigan State University, 1992 Lyte, Mark Research Interests: Animal nutrition, biosecurity, food Veterinary Microbiology and Preventive Medicine preservation and quality, grain and biomass analytics, Professor, W. Eugene Lloyd Chair in Toxicology sociopolitical implications Vet Med 2158, 515-294-5957 Research Description: Post-harvest engineering applied Email: [email protected] to grain and feed operations and processing, including loss Web: vetmed.iastate.edu/users/mlyte reduction and prevention, food security, grain operations Ph.D., M.S., MT(ASCP), Weizmann Institute of Science, management, feed technology, post-harvest engineering 1983 (crop handling, drying and storage), value-added processing Research Interests: Animal nutrition, neuroscience, mi- of agricultural crops and food/feed products, ecosystem crobiome, stress response in animals, stress response in modeling, stored products protection (IPM, fumigation), microorganisms alternative crop storage systems (grain chilling, hermetic Research Description: Examining the interface between storage), dehydration of food and biological products, bulk microbiology and neurobiology as regards behavior and material (grain, feed) handling and segregation (IP), quality health in both animals (i.e. farm-production) and humans. assurance of agricultural crops and biological products, and Including probiotic design and infectious disease aspects. facilities planning and design (including safety, entrapment rescue, dust explosion prevention, system simulation).

MacDonald, Ruth S. Food Science and Human Nutrition, Professor and Chair 2312 Food Sciences Building 515-294-5991 Fax: 515-294-8181 Email: [email protected] Web: fshn.hs.iastate.edu/directory/profile. php?u=ruthmacd&embedded=true Ph.D., University of Minnesota, 1985

33 Faculty Profiles Mair, Gunnar Maury, Wendy Biomedical Sciences; Assistant Professor Veterinary Microbiology and Preventive Medicine VetMed 2020 515-294-9568 Professor/Collaborator Email: [email protected] 3-750 Bowen Science Building 319-335-8021 PhD, Queen’s University Belfast, 1998 Fax: 319 335 9007 Research Interests: malaria, parasite transmission, gene Email: [email protected] expression control, cell biology Web: www.medicine.uiowa.edu/microbiology/profile/ wendy-maury Ph.D., University of Virginia, 1988 Mallapragada, Surya K. Research Interests: Animal cell culture, antibody diversity, Chemical and Biological Engineering antiviral agents, carbohydrates, gene expression, immu- Distinguished Professor, Carol Vols Johnson Chair nobiology, infectious diseases, molecular virology, tissue 2114 Sweeney Hall 515-294-7407 engineering, vaccines, virology Fax: 515-294-2689 Research Description: Host/virus interactions. How Email: [email protected] enveloped viral glycoproteins bind to and mediate entry Web: www.cbe.iastate.edu/the-department/ into permissive cells. Cellular attachment factors, recep- facultystaff/?user_page=suryakm tors, internalization pathways and fusion events used by Ph.D., Purdue University, 1996 a virus to enter cells. Development of antiviral therapies. Research Interests: Biomaterials, biomedical engineering, Ebola virus and Marburg virus, Zika virus. Identified a novel nanotechnology, pharmacokinetics, tissue engineering cell surface receptor for filoviruses, currently exploring the Research Description: Polymeric systems for controlled role of this family of cellular receptors in enveloped virus and targeted drug, vaccine and gene delivery. Polymeric pathogenesis. Elucidate the endosomal pathways used by scaffolds for tissue regeneration. filoviruses for entry.

Mansell, Thomas McGill, Jodi Chemical and Biological Engineering, Assistant Professor Veterinary Microbiology and Preventive Medicine 3035 Sweeney Hall 515-294-7177 Assistant Professor Email: [email protected] VMRI Building 5 515-249-2635 Ph.D., Cornell University, 2011 Email: [email protected] Research Interests: Antimicrobials, CRISPR/Cas9, carbo- Ph.D, University of Iowa hydrates, functional genomics, genome-editing nucleases, Research Interests: Veterinary immunology, respiratory metabolic engineering, microbiomes, protein engineering disease, vaccines and immunomodulators, innate immunity Research Description: Synthetic biology for microbiome Research Description: The focus of the McGill lab’s engineering. Prebiotics, probiotics, and antibiotics to shape research is to understand the mucosal immune response microbial populations. to respiratory infections in humans and animals, and to develop successful intervention strategies to promote protective immunity in the respiratory tract. Response to several respiratory pathogens of cattle, including bovine Martin, Richard respiratory syncytial virus, Mannheimia haemolytica and Biomedical Sciences, Professor and Chair, Mycobacterium bovis, the causative agent of bovine and Dr. E.A. Benbrook Chair in Pathology and Parasitology zoonotic tuberculosis. Nonconventional T cells and their 2008 Veterinary Medicine 515-294-2470 role in protection of the respiratory tract. Fax: 515-294-2315 Email: [email protected] Web: www.vetmed.iastate.edu/users/rjmartin Ph.D., University of Liverpool, 1977 McGrail, Maura A. Research Interests: Neuroscience, toxicology, cell biology Genetics, Development and Cell Biology Research Description: Anthelmintic drugs and mecha- Assistant Professor nisms of resistance. Physiology and pharmacology of C. 3005 Advanced Teaching and Research Building elegans and Ascaris suum nematode ion channels studied 515-294-4455 using patch-clamp techniques. Development of new target Email: [email protected] sites for anthelmintic drugs. Ph.D., University of Minnesota, 1996 Research Interests: Cancer, cancer genetics, neural developmental biology, functional genomics, gene-editing, CRISPR/Cas9, molecular genetics, conditional gene tools Research Description: Neural development and brain cancer in zebrafish using CRISPR/Cas9 gene editing and functional genomics.

34 Research in Biotechnology 2020 McNeill, Elizabeth Research Description: Investigation of the interface Food Science and Human Nutrition, Assistant Professor between segmented RNA viruses and the cells they infect, 1103 Human Nutritional Sciences Building 515-294-7421 particularly at the protein and nucleotide levels. Examina- Email: [email protected] tion of viral and cellular regulation of viral RNA transcrip- Ph.D., University of Wisconsin, Madison, 2009 tion, translation, and replication. Understanding the role of Research Interests: MicroRNA, synaptogenesis, synaptic spatial organization of viral replication and assembly into plasticity, neurodegenerative disease, tissue homeostasis, specialized viral and cellular protein-derived structures. Drosophila Utilization of a protein-protein interaction technology based Research Description: Investigation of the role of micro- on spatial localization of proteins within cells to identify and RNAs in formation and maintenance of neuronal connec- define new protein interactions, and screening for drugs tions. Exploring microRNA function in maintaining heart or small molecules which inhibit these interactions. Inves- muscle during aging using the genetic Drosophila model. tigation into the mechanisms of viral oncolysis of hypoxic tumor cells.

Mellata, Melha Food Science and Human Nutrition, Assistant Professor Miller, Les 3346 Food Sciences Building 515-294-9220 Computer Science Email: [email protected] 226 Atanasoff Hall 515-294-4337 Ph.D., Montreal University, 2004 Fax: 515-294-0258 Research Interests: Antimicrobials, food safety, infectious Email: [email protected] diseases, microbial virulence, microbiomes, vaccines Web: www.cs.iastate.edu/~lmiller Research Description: Pathogenesis of bacterial diseases Ph.D., Southern Methodist University, 1980 and emergence of antimicrobial resistance in bacteria, Research Interests: Computational biology, protein especially E. coli. Host-immune responses to infections structure and function and vaccination. Molecular pathogenesis and zoonotic Research Description: Development of a data warehouse mechanisms of food- and farm-associated bacteria. Effec- for computational biology. Predicting protein structure. tive intervention strategies. Vaccine development for food Applying machine learning techniques to biological data. safety, animal and human health. Miller, W. Allen Mendonca, Aubrey F. Plant Pathology and Microbiology, Professor; Roy J. Carver Food Science and Human Nutrition, Assistant Professor Department of Biochemistry, Biophysics and Molecular 2312 Food Sciences Building 515-294-2950 Biology; Professor Fax: 515-294-8181 413 Bessey Hall 515-294-2436 Email: [email protected] Fax: 515-294-9420 Ph.D., Iowa State University, 1992 Email: [email protected] Research Interests: Antimicrobials, food safety, stress Web: www.plantpath.iastate.edu/millerlab/ response in microorganisms Ph.D., University of Wisconsin, 1984 Research Description: Use of natural antimicrobials for Research Interests: Bioinformatics, gene expression, enhancing the destructive effect of heat, irradiation or high molecular virology, nucleic acids, plant cell culture, plant- hydrostatic pressure against food-borne human enteric pest interactions, protein structure and function, RNA pathogens. Influence of stress-adaptation on bacterial structure and function, transgenic plants, virology, resistance to food processing methods. structural biology, phenomics Research Description: Mechanisms of novel translation initiation and recoding events used by RNA viruses. RNA virus discovery by deep sequencing. Ribosome profiling. Miller, Cathy L. Engineering plant viruses as expression vectors. Aphid Veterinary Microbiology and Preventive Medicine, transmission of plant viruses. Interim Department Chair, Assistant Professor Veterinary Medicine Institute I 515-294-4797 Fax: 515-294-1401 Email: [email protected] Ph.D., University of Missouri-Columbia Medical School, 2001 Research Interests: Animal protection from infectious disease, antiviral agents, cancer, cell biology, imaging technology, molecular virology, protein structure and function, proteomics, RNA structure and function, vector-borne diseases, virology

35 Faculty Profiles Miner, Andrew S. Montazami, Reza Computer Science, Associate Professor Mechanical Engineering, Assistant Professor 233 Atanasoff 515-294-2392 2094 Black Engineering 515-294-8733 Email: [email protected] Email: [email protected] Web: www.cs.iastate.edu/~asminer Web: web.me.iastate.edu/montazami/ Ph.D., College of William and Mary, 2000 Ph.D., Virginia Polytechnic Institute, 2011 Research Interests: Nanotechnology Research Interests: Functional materials, biomaterials, Research Description: Modeling and analysis of DNA nanotechnology, biomedical engineering. nanotechnology using chemical reaction networks and Research Description: Design, fabrication and character- Petri nets. ization of soft bio-electronic devices. Synthesis and characterization of functional soft materials. Development of soft microrobotics for biomedical devices. Minion, Chris Veterinary Microbiology and Preventive Medicine Professor Moorman, Thomas B. 1130 Veterinary Medicine 515-294-6347 Agronomy, Associate Professor, USDA Collaborator Fax: 515-294-8500 National Laboratory for Agriculture and the Environment, Email: [email protected] Room 318 515-294-2308 Web: vetmed.iastate.edu/users/fcminion Fax: 515-294-8125 Ph.D., University of Alabama, Birmingham, 1983 Email: [email protected] Research Interests: Animal protection from infectious dis- Ph.D., Washington State University, 1983 eases, microbial genetics, bioinformatics, gene expression, Research Interests: Bioreactors, bioremediation of envi- genomics, immunobiology, microbial virulence, molecular ronmental pollutants, antimicrobials, soil-plant interactions genetics of microorganisms, nucleic acids, proteomics, Research Description: Pathways and rates of pesticide vaccines and antibiotic biodegradation in soils and stream sedi- Research Description: Study of Mycoplasma pathogen- ments. Management of excess nitrate in agricultural water- esis at the protein and genetic levels. Testing of new vac- sheds. Detection of antibiotic-resistant microorganisms in cines and therapeutic delivery systems. manure, manure-treated soils and streams. Investigation of rhizosphere microbiology of cropping systems.

Mochel, Jonathan Paul Biomedical Sciences, Associate Professor; Mooyottu, Shankumar Veterinary Diagnostics and Production Animal Medicine, Veterinary Pathology, Assistant Professor, Associate Professor 2716 Veterinary Medicine 515-294-3877 2448 Veterinary Medicine 515-294-7424 Email: [email protected] Email: [email protected] Web: vetmed.iastate.edu/users/shaan DVM, MSc, Ph.D., Dipl.ECVPT, Leiden University, 2007, DVM, Ph.D. DACVP, University of Connecticut, 2016 2009, 2013, 2015 Research Interests: C. difficile, gut microbiome, antibiotic Research Interests: Animal physiology, antibiotic resistance, molecular epidemiology resistance, antimicrobials, Big Data, biostatistics, cancer, Research Description: Development of novel non-antibiot- clinical pharmacology, computational biology, human ic strategies for controlling important enteric diseases such physiology, molecular dynamics, molecular mechanisms, as C. difficile infection (CDI) in humans and animals with pharmacokinetics, toxicology special reference to gut-microbiome and gut-dysbiosis. Research Description: Foster synergistic research be- Identification of gut-friendly small molecules that could tween human health and animal health in order to improve potentially be used for preventing gut-dysbiosis and associ- health outcomes and well-being of humans and animals. ated enteric diseases. Molecular epidemiology and poten- Bridge the knowledge gap between experimental murine tial zoonotic or foodborne transmission of CDI in humans. models and human patients and support the selection of the most promising clinical candidates with high probability of success, both from an efficacy and safety standpoint. Use modeling and simulation approaches for translational (animal to human) and reverse translational (human to animal) medical research.

36 Research in Biotechnology 2020 Moschini, GianCarlo Research Interests: Biosecurity, epidemiology, integrated Economics, Professor pest management, microbial virulence, plant-pest interac- 583 Heady Hall 515-294-5761 tions, toxicology, transgenic plants Fax: 515-294-6336 Research Description: Biology and epidemiology of Email: [email protected] plant diseases affecting corn and soybean crops. Occur- Web: www.econ.iastate.edu/faculty/moschini rence and management of toxigenic fungi affecting crop Ph.D., University of Guelph, 1986 plants and plant products, including co-products of ethanol Research Interests: Economic implications processing. Impacts of biotechnology on plant diseases. Research Description: Biofuel policies, biotechnology, Detection and risk assessment of seedborne pathogens. incentives for innovation, intellectual property rights, R&D, regulatory policies, risk. Myers, Alan M. Roy J. Carver Department of Biochemistry, Biophysics and Moss, Walter Molecular Biology; Professor Roy J. Carver Department of Biochemistry, Biophysics and 2110 Molecular Biology Building 515-294-9548 Molecular Biology; Assistant Professor Fax: 515-294-0453 3256 Molecular Biology Building 515-294-6214 Email: [email protected] Email: [email protected] Web: www.bbmb.iastate.edu/people/alan-myers Web: www.mosslab.org/ Ph.D., Duke University, 1983 Ph.D., University of Rochester, 2012 Research Interests: Carbohydrates, metabolic engineer- Research Interests: Bioinformatics, cancer, infectious ing, molecular genetics of plants diseases, molecular virology, nucleic acids, RNA structure Research Description: Maize endosperm metabolism. and function Research Description: Identify RNA sequences with a high propensity to form structure; deduce their structures, Narasimhan, Balaji functions, and the roles played by structure. Chemical and Biological Engineering Anson Marston Distinguished Professor 2035 Sweeney Hall 515-294-8019 Most, Corinna Email: [email protected] Ecology, Evolution and Organismal Biology Web: www.cbe.iastate.edu/narasimhan Adjunct Assistant Professor Ph.D., Purdue University, 1996 Bessey 131 858-652-0873 Research Interests: Engineered biomaterials, nanovac- Email: [email protected] cines, nanomedicine, drug and vaccine delivery Ph.D., University of California San Diego, 2018 Research Description: Molecular design of nanostructured Research Interests: Primate behavioral ecology, infant polymer systems and engineered biomaterials, controlled development, onteogeny and phylogeny of social behavior delivery of drugs, proteins, and vaccines, and combinatorial Research Description: Development of social behavior materials science. and the acquisition of social skills in wild olive baboons in Laikipia, Kenya. In particular, the effects of maternal behavior and of relationships between infants and individuals Nason, John other than the mother on infant development. The manner Ecology, Evolution and Organismal Biology in which environmental changes in the area - specifically Associate Professor and Chair the spread of an invasive plant species that has become a 341 Bessey Hall 515-294-2268 favorite baboon food - influence patterns of female repro- Fax: 515-294-1337 duction, maternal behavior, and infant development. Use of Email: [email protected] methods and theories from human development research Ph.D., University of California, Riverside, 1991 to draw comparisons between the processes observed in Research Interests: Animal genetics, molecular evolution, baboons and those present in our own species. evolutionary biology, plant genetics, plant-pest interactions Research Description: Comparative population genetic structure, phylogeography and diversification of coevolving Munkvold, Gary plant/insect interactions. Host race formation in herbivo- Plant Pathology and Microbiology rous insects. Genetic and reproductive consequences of Professor and Seed Science Endowed Chair habitat fragmentation. 160 Seed Science 515-294-7560 Fax: 515-294-2014 Email: [email protected] Ph.D., University of California, Davis, 1992

37 Faculty Profiles Neihart, Nathan M. Nikolau, Basil J. Electrical and Computer Engineering, Assistant Professor Roy J. Carver Department of Biochemistry, Biophysics 2132 Coover Hall 515-294-5709 and Molecular Biology; Frances F. Craig Professor; Center Fax: 515-294-3637 for Metabolic Biology, Director; Center for Biorenewable Email: [email protected] Chemicals, Deputy Director Web: www.ece.iastate.edu/~neihart 3254 Molecular Biology Building 515-294-9423 Ph.D., University of Washington, 2008 Fax: 515-294-0453 Research Interests: Biomedical engineering, systems Email: [email protected] biology Ph.D., Massey University, New Zealand, 1982 Research Description: Development and design of low Research Interests: Biorenewables, enzyme mechanisms, power interface circuit design for biomedical applications. gene expression, genomics, lipids, metabolic engineering, Development of low power, high dynamic range sensor metabolomics, molecular genetics of plants, seed physiol- interface circuits for bio-sensing applications. ogy, transgenic plants, vitamins and minerals Research Description: Molecular biology and genetics of metabolism. Biochemical and genetic regulation of metabo- Nelson, Scott W. lism. Metabolic networking. Lipid metabolism. Molecular Roy J. Carver Department of Biochemistry, Biophysics and biology of vitamins. Metabolic diversity. Biocatalysis. Molecular Biology; Assistant Professor 4112 Molecular Biology 515-294-3434 Fax: 515-294-0453 Nilsen-Hamilton, Marit Email: [email protected] Roy J. Carver Department of Biochemistry, Biophysics and Ph.D., Iowa State University, 2002 Molecular Biology; Professor Research Interests: Antimicrobials, enzyme mechanisms, 3206 Molecular Biology Building 515-294-9996 infectious diseases, protein structure and function, nucleic Fax: 515-294-0453 acids, cancer Email: [email protected] Research Description: Molecular mechanisms of key en- Ph.D., Cornell University, 1973 zymes involved in DNA replication and repair. Structure and Research Interests: Nucleic acids, gene expression, function analysis of the Mre11/Rad50 DNA repair complex. protein structure and function, animal cell culture, animal Enzymology of DNA polymerases. hormones and peptide growth factors, cancer, cell biology Research Description: Nucleic acid aptamers as probes for imaging in molecular analysis and in medicine. Nettleton, Dan Bio-based materials development. Mechanisms by which Statistics, Distinguished Professor growth factors regulate genes in mammalian cells. 1121H Snedecor 515-294-7754 Fax: 515-294-4040 Email: [email protected] O’Neal, Matthew Web: dnett.github.io/ Entomology, Assistant Professor; Graduate Program in Distinguished Professor, University of Iowa, 1996 Sustainable Agriculture, Chair Research Interests: Bioinformatics, biostatistics, gene ex- 117 Insectary 515-294-8622 pression, genomics, phenomics, proteomics, quantitative Fax: 515-294-7406 genetics, transcriptome analysis Email: [email protected] Research Description: Developing statistical methods for Ph.D., Michigan State University, 2002 the analysis of omics data. Research Interests: Integrated pest management, plant- pest interactions Research Description: Response of soybeans to insect Niemi, Jarad pests, including the recent invasive soybean aphid Aphis Statistics, Associate Professor glycines and the native bean leaf beetle Ceratoma trifurca- 2208 Snedecor 515-294-8679 ta. Impact of plant health on insect olfactory response and Email: [email protected] population growth. Web: jarad.me Ph.D., Duke University, 2009 Research Interests: Biostatistics, computational biology, epidemiology, systems biology, transposable elements, vaccines Research Description: Developing statistical methods for the analysis of intracellular protein dynamics and disease prevention, outbreak detection, and control.

38 Research in Biotechnology 2020 Ong, Say Kee Palerme, Jean-Sebastien Civil, Construction and Environmental Engineering Veterinary Clinical Sciences, Assistant Professor Professor Rm 2522 Lloyd Veterinary Medical Center 515-294-8790 486 Town Engineering 515-294-3927 Email: [email protected] Fax: 515-294-8216 DVM, University of Montreal, 2008 Email: [email protected] Research Interests: Infectious diseases, immunobiology, Web: www.ccee.iastate.edu/facultystaff/?user_ vector-borne disease page=skong Research Description: Clinical research in small animal Ph.D., Cornell University, 1990 veterinary medicine with an interest in infectious diseases Research Interests: Bioremediation of environmental and minimally-invasive diagnostics and therapeutics. pollutants, waste management Research Description: Biological and physical-chemical degradation of organic compounds, pharmaceutical drugs Pandey, Santosh and pesticides in wastewater, soils and sediments. Biore- Electrical and Computer Engineering, Associate Professor mediation technologies for wastewater and contaminated 2126 Coover Hall 515-294-7504 soils. Natural attenuation of organic compounds. Email: [email protected] Ph.D., Lehigh University, 2006 Research Interests: Microfluidics, stress response in Opriessnig, Tanja plants, stress response in animals, stress response in Veterinary Diagnostic and Production Animal Medicine microorganisms, imaging technology Professor Research Description: Smart sensor platforms for real- Veterinary Medicine Institute, Building 1, 1802 University time study of spatial cell growth, intercellular communica- Boulevard 515-294-1137 tion, functionality of neural networks, planar patch-clamp- Fax: 515-294-6961 ing, electroporation/sonoporation, membrane transport and Email: [email protected] nanofabrication. Web: vetmed.iastate.edu/users/tanjaopr Doctora medicinae veterinariae (DVM equivalent), Ph.D., University of Veterinary Medicine Vienna, Austria, Iowa Parshad, Rana State University, 2000, 2002, 2006 Mathematics, Assistant Professor Research Interests: Animal protection from infectious 456 Carver Hall 515-294-7294 diseases, antiviral agents, molecular virology, vaccines, Email: [email protected] virology Web: rparshad.wixsite.com/mbio Research Description: Pathogenesis, diagnosis, treatment Ph.D., Florida State University, 2009 and protection against swine infectious diseases. Develop- Research Interests: Mathematical biology, population ment of new diagnostic tools for pathogens of veterinary dynamics importance.

Peschel, Joshua Padalkar, Sonal Agricultural and Biosystems Engineering Mechanical Engineering, Assistant Professor Assistant Professor 2068 Black Engineering 515-294-6066 2348 Elings Hall (515) 294-4814 Email: [email protected] Email: [email protected] Web: www.me.iastate.edu/directory/faculty/sonal-padalkar/ Web: www.peschelgroup.org Ph.D., Purdue University, 2010 Ph.D., Texas A&M University, 2012 Research Interests: Biomaterials, energy development, Research Interests: Animal production systems, Big microfluidics, nanotechnology, peptide growth factors Data, biosensors, cyber-physical-human systems, machine Research Description: Synthesis, characterization and learning, optical imaging, phenomics, precision agriculture, simulation of hybrid nanostructures for energy-related precision animal science, remote sensing, robotics, socio- application. Synthesis and characterization of nanomateri- political implications, unmanned systems als for biosensing. Characterization techniques like TEM, Research Description: Creating new technologies, data HRTEM, EELS and Atom Probe to obtain solutions for sets, and predictive models for sensing and sensemaking complex research problems. Bottom synthesis techniques to advance cyber-physical-human interaction in agricultural, for nanoelectronic applications. Design, synthesis and char- natural, and urban systems. acterization for photoanodes for hydrogen generation.

39 Faculty Profiles Peters, Reuben Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology; Roy J. Carver Professor 4216 Molecular Biology Building 515-294-8580 Phillips, Gregory J. Fax: 515-294-0453 Veterinary Microbiology and Preventive Medicine Email: [email protected] Professor Ph.D., University of California, San Francisco, 1998 202 Veterinary Medical Research Institute, Building 6 Research Interests: Biochemical engineering, biore- 515-294-1525 newables, enzyme mechanisms, genomics, metabolic Email: [email protected] engineering, molecular evolution, natural products, plant Web: vetmed.iastate.edu/users/gregory hormone synthesis and action, protein engineering, protein Ph.D., University of Georgia, 1987 structure and function Research Interests: Microbial genetics, gene expression, Research Description: Investigation of natural products genomics, microbial virulence, molecular genetics of micro- biosynthesis, as well as physiological function, spanning a organisms, RNA structure and function, vaccines, metage- range of systems that include medicinal herbs, cereal crop nomics, functional genomics plant defense, rhizobacteria-legume symbiosis and various Research Description: Microbiomes. Protein targeting to bacterial plant-pathogens (e.g., Xanthomonas), and the hu- bacterial membranes, microbial antibiotic resistance and man pathogen Mycobacterium tuberculosis. tolerance and bacterial genomics.

Peterson, Thomas Pineyro, Pablo Genetics, Development and Cell Biology, Professor Veterinary Diagnostic and Production Animal Medicine 2258 Molecular Biology Building 515-294-6345 Assistant Professor Email: [email protected] 1655 Veterinary Diagnostic Laboratory 515-294-6250 Ph.D., University of California, 1984 Email: [email protected] Research Interests: Gene expression, molecular genetics Web: vetmed.iastate.edu/users/pablop of plants, plant genetics, transposable elements, transgen- DVM, MVSc, DVSc, Ph.D., Iowa State University, ic plants, molecular cytogenetics Research Interests: Infectious diseases, molecular Research Description: Plant molecular genetics and cyto- virology, vaccines, virology genetics, including transposable elements and their effects Research Description: Diagnostic assay development on gene expression and genome structure. and basic pathogenesis of viral infectious disease affecting swine production systems. Porcine Epidemic Diarrhea Virus (PEDV) host-pathogen interaction and development of Petrich, Jacob W. diagnostic assay and pathogenesis of Senecavirus A (SVA). Chemistry, Professor Collaborative projects on Porcine Circovirus Type 1 (PCV1), 0773 Gilman Hall 515-294-9422 Porcine Circovirus Type 2 (PCV2), and Porcine Reproduc- Fax: 515-294-0105 tive and Respiratory Syndrome Virus (PRRSV). Email: [email protected] Web: www.chem.iastate.edu/people/jacob-petrich Ph.D., University of Chicago, 1985 Plummer, Paul J. Research Interests: Antiviral agents, food safety, protein Veterinary Diagnostic and Production Animal Medicine structure and function Associate Professor Research Description: Development and application of ul- 2426 Veterinary Medicine Building 515-294-8522 trafast laser spectroscopies and related optical techniques Email: [email protected] to study and characterize systems of biological relevance. DVM, Ph.D., University of Tennessee, Iowa State Light-induced antitumor and antiviral agents. Remote sens- University, 2000, 2009 ing of environmental contaminants. Excited-state proton Research Interests: Animal protection from infectious and charge transfer; solvation dynamics in protein environ- diseases, antimicrobials, antibiotic resistance, food safety, ments. immunobiology, microbial virulence, molecular genetics of microorganisms Research Description: Bacterial pathogens associated with disease in food production animals or food safety. Molecular and traditional microbiological techniques to facilitate the development of intervention strategies useful in decreasing animal disease and improving food safety. Mechanisms and ecology of antimicrobial resistance in animals and humans.

40 Research in Biotechnology 2020 Potoyan, Davit Qiu, Yumou Chemistry, Assistant Professor Statistics, Assistant Professor 2759 Gilman Hall (515)-294-9971 3214 Snedecor Hall 515-294-0212 Email: [email protected] Email: [email protected] Web: group.chem.iastate.edu/Potoyan/index.html Ph.D., Iowa State University, 2014 Ph.D., University of Maryland, College Park, 2012 Research Interests: High-dimensional data analysis, infer- Research Interests: Theoretical and computational ence for large covariance and precision matrices, high- biophysics throughput phenotyping Research Description: Assembly of proteins and nucleic Research Description: High-dimensional data analysis acids, mesoscale reaction-diffusion in cells, genetic regula- and application to genetic studies, correlation and partial tory networks. correlations among genes, imaging data analysis for high- throughout phenotyping data, functional data analysis for plant growth. Powell-Coffman, Jo Anne Genetics, Development and Cell Biology, Professor; Associate Dean for Research in the College of Liberal Arts Que, Long and Sciences Electrical and Computer Engineering, Associate Professor 2108 Molecular Biology Building 515-294-3906 2113 Coover Hall 515-294-6951 Email: [email protected] Email: [email protected] Ph.D., University of California, San Diego, 1993 Ph.D., University of Wisconsin-Madision, 2000 Research Interests: Animal genetics, cancer, developmen- Research Interests: Biomedical engineering, microfluidics, tal biology, gene expression, signal transduction, stress nanotechnology, optical imaging response in animals, functional genomics, microfluidics, Research Description: Screening and detection of diseas- molecular genetics of animals, systems biology, toxicology es, including cancers, at early stage using nano-photonic Research Description: Adaptation to hypoxia (low oxy- biosensors. Single cell analysis using patch-clamp microflu- gen), with a focus on the hypoxia-inducible factor and the idic chips. DNA sequencing and single molecule imaging aryl hydrocarbon receptor transcription factors. Develop- using nanostructured fluorescence sensing technology. mental genetics in C. elegans. Application of nanotechnology and optical technology for cell transfection.

Prusa, Kenneth J. Food Science and Human Nutrition, Professor Raman, D. Raj 220 MacKay Hall 515-294-4323 Agricultural and Biosystems Engineering, Morrill Professor Fax: 515-294-2215 3356 Elings Hall 515-294-0465 Email: [email protected] Email: [email protected] Ph.D., Kansas State University, 1983 Ph.D., Cornell University, 1994 Research Interests: Food quality evaluation, sensory Research Interests: Perennial groundcovers, biorenew- evaluation methodology, pork quality measurements ables, fermentation, STEM education Research Description: Composition, sensory quality and Research Description: Techno-economic and lifecycle physical properties of meat and meat products. assessment of biorenewable resource production and processing systems.

Putz, Austin Animal Science Ramirez, Alejandro 239C Kildee Hall 515-249-6638 Veterinary Diagnostic and Production Animal Medicine, Email: [email protected] Associate Professor, Roy A. Schultz Professorship in Swine Ph.D., Iowa State University, 2019 Medicine Research Interests: Quantitative genetics, animal breed- 2231 Lloyd Vet Med Center 515-294-7463 ing, resilience in pigs, pigs, computational aspects of Fax: 515-294-1072 animal breeding Email: [email protected] Research Description: Computational swine genetics, as DVM, MPH, Ph.D., Iowa State University, 1993, 2004, well as all aspects of swine animal husbandry. 2011 Research Interests: Animal protection from infectious diseases, biosecurity, epidemiology, infectious diseases, vaccines Research Description: Infectious swine disease transmission and diagnostic tools and interpretation. Within-herd dynamics of disease transmission related to PRRSV, as

41 Faculty Profiles well as the use of oral fluids for surveillance monitoring of Reecy, James other viral agents. Improved design of testing schemes, as Office of the Vice President for Research well as how to better interpret multi-agent diagnosis from Associate Vice President; Animal Science, Professor a population standpoint. Developed a piglet model for Clos- 2810 Beardshear Hall 515-294-1609 tridium difficile studies. Using Mycoplasma hyopneumoni- Fax: 515-294-2401 ae and M. hyosynoviae to better understand prevalence, Email: [email protected] diagnostics and clinical significance. Special emphasis in Web: www.ans.iastate.edu/people/james-reecy the area of infectious disease zoonosis. Evidence-based Ph.D., Purdue University, 1995 medicine and how to better evaluate studies to improve Research Interests: Animal cell culture, animal genetics, the reporting of studies. animal protection from infectious disease, bioinformatics, cell biology, developmental biology, gene expression, genomics, molecular genetics of animals, muscle biology, Rao, Guru signal transduction, transgenic animals, transcriptome Office of the Vice President for Research, Associate Vice analysis President for Research; Roy J. Carver Department of Research Description: Identification of molecular mecha- Biochemistry, Biophysics and Molecular Biology; Professor nisms that control skeletal muscle growth and develop- 4212 Molecular Biology Building 515-294-6116 ment, meat quality, nutrient composition of meat, and Fax: 515-294-0453 disease resistance. Development of bioinformatics tools to Email: [email protected] facilitate livestock genomics. Ph.D., University of Mysore (India), 1981 Research Interests: Proteomics, protein engineering, protein structure and function, signal transduction Ren, Juan Research Description: Broad interest in protein science, Mechanical Engineering, Assistant Professor with an emphasis on the biochemical characterization of 2030 Black Engineering 515-294-1805 proteins to establish structure-activity relationships, protein Email: [email protected] engineering for altering protein function, protein folding, Web: www.me.iastate.edu/directory/faculty/juan-ren/ protein stability and protein-protein interactions. Study of Ph.D., Rutgers Unviersity, 2015 the cell surface receptor Crinkly4 in Arabidopsis (ACR4) Research Interests: Biomaterials, cell biology, live-cell which encodes a tumor necrosis factor-like (TNFR-like) imaging, nanotechnology, STEM education receptor kinase. Applying biochemical and biophysical Research Description: Nanoscale probe-based broadband techniques to understand the structure-function properties biomechanics characterization. High-speed imaging and of ACR4 and to delineate the downstream protein-protein broad-band viscoelasticity spectroscopy of soft and biologi- interactions mediated by the kinase domain. Elucidation of cal materials. the signal transduction pathways in seed development to provide key insights into novel strategies to produce grain for food, feed and renewable fuels. Reuel, Nigel Chemical and Biological Engineering, Assistant Professor 3051 Sweeney 515-441-9169 Reddy, Manju Email: [email protected] Food Science and Human Nutrition, Professor Web: reuelgroup.org 1107 Human Nutritional Sciences Building 515-294-2024 Ph.D., Massachusetts Institute of Technology, 2014 Fax: 515-294-5390 Research Interests: Antibody structure-function, Email: [email protected] biomaterials, biosensors, carbohydrates, diagnostics, Ph.D., Texas A & M University, 1987 enzyme mechanisms, microfluidics, nanotechnology, pro- Research Interests: Bioavailability of nutrients, human tein structure and function nutrition and health, vitamins and minerals Research Description: Characterization, synthesis and Research Description: Human studies using stable iso- applications of protein-based bio-materials, hydrolytic tope methodology to measure iron absorption from novel enzyme switches, and contact-free, label-free biosensors iron compounds used in supplementation and fortification. for plant, animal, and human health and physiology. Cell culture model to screen food iron bioavailability from various food combinations to identify best strategies to reduce the prevalence of anemia. Safe iron supplementation in various diseases. Understanding the alteration of iron metabolism in Parkinson’s disease and assessing the protective effect of green tea polyphenols and phytic acid in preventing the progression of the disease in humans, animals and cell culture models.

42 Research in Biotechnology 2020 using a variety of molecular markers, including genomic Robertson, Alison data (RAD Seq), DNA sequence data, and micro satellites. Plant Pathology and Microbiology, Associate Professor 2203 Advanced Teaching and Research Building 515-294-6780 Roof, Michael Fax: 515-294-1741 Veterinary Microbiology and Preventive Medicine, Email: [email protected] Collaborator/Professor Ph.D., Clemson University, 2003 2412 South Loop Drive 515-294-6746 Research Interests: Plant-pest interactions, epidemiology, Fax: 515-294-7937 integrated pest management, microbial virulence Email: [email protected] Research Description: Biology and epidemiology of Ph.D., Iowa State University, 1991 oomycete pathogens of corn and soybean. Effect of crop Research Interests: Animal cell culture, animal protection management practices on seedling diseases of corn. Role from infectious diseases, antiviral agents, microbial of allelopathy in seedling disease of corn. Genetic diversity genetics, vaccines, immunobiology, microbial virulence, of and molecular characterization of avirulence genes in molecular genetics of microorganisms, molecular virology Phytophthora sojae. Research Description: Bacterial and viral infectious agents associated with clinical disease in animals. Virulence factions and key immunogens. Novel, non-needle delivery Roche, Julien of vaccines. Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology; Assistant Professor 3116 Molecular Biology Bulding 515-294-6437 Rosentrater, Kurt A. Email: [email protected] Agricultural and Biosystems Engineering Web: www.bbmb.iastate.edu/people/julien-roche Assistant Professor Ph.D., Science University of Montpellier, FRA, 2012 3327 Elings Hall 515-294-4019 Research Interests: Structural biology Email: [email protected] Research Description: Protein folding and characterization Web: www.public.iastate.edu/~karosent/ of disordered proteins involved in neurological diseases Ph.D., Iowa State University, 2001 using NMR. Research Interests: Animal nutrition, biomaterials, bioreactors, biorenewables, carbohydrates, economic implications, energy development, fermentation, food Rodermel, Steven processing, grain and biomass analytics, human nutrition Genetics, Development and Cell Biology, Professor and health, plant protein and oil utilization, product separa- 467 Bessey Hall 515-294-8890 ration/recovery, waste management Fax: 515-294-1337 Research Description: Improving the sustainability of Email: [email protected] biorenewable resources, including the development of Ph.D., Harvard University, 1986 value-added materials, processes, and coproducts. Research Interests: Developmental biology, gene Improvements in processing efficiencies, techno-economic expression, metabolic engineering, molecular genetics of and life cycle assessments. plants, organelle genetics, protein structure and function Research Description: Chloroplast biogenesis. Carotenoid biosynthesis and alternate electron sinks. Arabidopsis Ross, Jason W. variegation mutants and mechanisms of nuclear-organelle Animal Science, Lloyd L. Anderson Professor in Physiology interactions. Chloroplast proteomics. Iowa Pork Industry Center, Director 2356E Kildee Hall 515-294-8647 Fax: 515-294-4471 Roe, Kevin J. Email: [email protected] Natural Resource Ecology and Management, Web: www.ans.iastate.edu/people/jason-ross Adjunct Assistant Professor Ph.D., Oklahoma University, 2006 339 Science II 515-294-8332 Research Interests: Animal cell culture, animal embryos, Fax: 515-294-2995 pig reproduction, molecular genetics of animals, animal Email: [email protected] reproductive efficiency Web: www.nrem.iastate.edu/research/roe/ Research Description: Basic and applied swine reproduc- Ph.D., University of Alabama, 1999 tion and biotechnology. Understanding the molecular and Research Interests: Evolutionary biology, molecular physiological factors governing reproduction. genetics of animals, molecular ecology Research Description: Conservation genetics, evolutionary relationships, and biogeography of aquatic organisms

43 Faculty Profiles Roth, James A. Sacco, Randy Veterinary Microbiology and Preventive Medicine, Veterinary Microbiology and Preventive Medicine Distinguished Professor Associate Professor; USDA, Collaborator 2156 Veterinary Medicine 515-294-8459 National Animal Disease Center 515-337-7354 Fax: 515-294-8259 Fax: 515-337-7428 Email: [email protected] Email: [email protected] DVM, Ph.D., Iowa State University, 1975, 1981 Ph.D., Texas A & M University, 1987 Research Interests: Animal hormones and peptide growth Research Interests: Cell biology, immunobiology, B cell factors, animal protection from infectious diseases, immu- immunity nobiology, microbial virulence, stress response in animals, Research Description: Modulation of host immune vaccines responses following infection with respiratory pathogens; Research Description: Cell-mediated immunity to viral interaction between innate and adaptive immunity; anti- and bacterial pathogens and vaccines of cattle and swine. gen presenting cells including B cells, macrophages, and Development and licensing of vaccines for emerging and dendritic cells. exotic diseases of domestic animals.

Sahin, Orhan Rothschild, Max F. Veterinary Diagnostic and Production Animal Medicine Animal Science, Charles F. Curtiss Distinguished Professor Assistant Professor in Agriculture and Life Sciences, M. E. Ensminger Chair 1583 Veterinary Medicine 515-294-3861 in International Animal Agriculture; Center for Sustainable Fax: 515-294-8500 Rural Livelihoods, Associate Director Email: [email protected] 2255 Kildee Hall 515-294-6202 Ph.D., The Ohio State University, 2003 Fax: 515-294-2401 Research Interests: Comparative genomics, epidemiology, Email: [email protected] food safety, genomics, microbial genetics, microbial ecol- Web: www.ans.iastate.edu/people/max-rothschild ogy, microbial identification, proteomics, stress response in Ph.D., Cornell University, 1978 microorganisms, systems biology, vaccines, vector-borne Research Interests: Animal breeding, animal genetics, disease gene expression, genomics, immunobiology Research Description: Development of effective vac- Research Description: Gene identification in the pig and cines and antimicrobials for bacterial pathogens of humans other livestock species including cattle, sheep, goats, and animals. Molecular epidemiology and antimicrobial chickens, horse and camel. Identification of genes affect- resistance mechanisms of foodborne bacterial pathogens ing economic traits, including performance, reproduction, including Campylobacter in animal reservoirs. Pathogenesis health and meat quality traits. Genome-wide association and immunobiology of bacterial infections in animals and studies and QTL analysis and identification. Role of MHC humans. Comparative and functional genomics of bacterial genes in pig health. Genetics of dogs and cats. Sequenc- organisms. ing of sheep, pig, goat and shrimp. Genomic development projects in Africa. Sakaguchi, Donald S. Genetics, Development and Cell Biology, Morrill Professor Rowling, Matthew 502 Science II 515-294-3112 Food Science and Human Nutrition, Assistant Professor Fax: 515-294-8457 1103 Human Nutritional Sciences 515-337-9105 Email: [email protected] Fax: 515-294-6193 Web: dssakagu.public.iastate.edu/Sakaguchi/ Email: [email protected] Ph.D., State University of New York, Albany, 1984 Ph.D., Iowa State University, 2004 Research Interests: Animal cell culture, animal embryos, Research Interests: Human nutrition and health, bioethics, cell biology, developmental biology, drug deliv- oncogenes, human physiology, vitamins and minerals, cell ery, monoclonal antibodies, nanotechnology, neuroscience, biology, cancer signal transduction, stem cells, tissue engineering Research Description: Investigation of nutrient traffick- Research Description: Stem cell development, differentia- ing mechanisms in tissues prone to tumor formation with tion and plasticity. Experimental strategies for brain rescue specific interest in breast, prostate and colon. Understand- and repair. Advanced retinal therapies. Nerve regeneration. ing how tissue concentrations of anti-tumorigenic nutrients Stem cell biology. (specifically vitamins A and D) can be optimized to reduce cancer incidence. Interactions between type 2 diabetes and vitamin D status. Optimization of vitamin D nutrition in type 2 diabetics in order to reduce the incidence of cancers that have strongly been associated with type 2 diabetes.

44 Research in Biotechnology 2020 Salas Fernandez, Maria Sato, Yuko Agronomy, Associate Professor Veterinary Diagnostic and Production Animal Medicine 1126E Agronomy Hall 515-294-9563 Assistant Professor Email: [email protected] Lloyd Veterinary Medical Complex 2430 515-294-0710 Web: faculty.agron.iastate.edu/mgsalas/ Email: [email protected] Ph.D., Cornell University, 2008 Web: register.extension.iastate.edu/eggsymposium Research Interests: Gene expression, genomics, mo- DVM, Purdue University, 2012 lecular genetics, molecular genetics of plants, molecular Research Interests: Extension, biosecurity, infectious mechanisms, phenomics, photosynthesis and photores- diseases piration, plant breeding, plant genetics, plant physiology, Research Description: Avian influenza, clostridial dermati- quantitative genetics, stress response in plants, transgenic tis in turkeys, laying hen diseases, current/emerging infec- plants tious diseases, public/producer outreach. Research Description: Development of superior sorghum lines as lignocellulosic feedstock for biofuel production. Discovery of genes associated with traits of economic Schalinske, Kevin L. importance including photosynthesis, photoprotection, Food Science and Human Nutrition, Professor plant architecture, grain yield and abiotic stress tolerance. 220 MacKay Hall 515-294-9230 Exploitation of candidate genes for food, feed, fiber and Fax: 515-294-6193 fuel production by genetic engineering or gene editing. Email: [email protected] Development and use of high-throughput phenotyping Web: fshn.hs.iastate.edu/directory/profile. technologies for genetic studies. php?u=kschalin&embedded=true Ph.D., University of Wisconsin, 1992 Research Interests: Cancer, cell biology, developmental Sappington, Thomas W. biology, gene expression, human nutrition and health, Entomology, USDA-ARS, Affiliate Professor nucleic acids, oncogenes, toxicology, vitamins and minerals 109 Genetics Laboratory 515-294-9759 Research Description: Nutritional biochemistry and Fax: 515-294-9359 molecular biology applied to the role of nutrients and Email: [email protected] nutrient interactions in health and disease. Determining Web: www.ent.iastate.edu/people/thomas-sappington how various factors can adversely regulate the folate-de- Ph.D., University of Kansas, 1989 pendent, one-carbon pool and methyl group metabolism to Research Interests: Evolutionary biology, genomics, contribute to disease. integrated pest management, molecular ecology, molecular evolution, plant-pest interactions, transgenic plants Research Description: Population genetics and molecular Schmitz-Esser, Stephan ecology of insect pests of corn. Insect migration, dispersal, Animal Science, Associate Professor and gene flow in the context of insect resistance manage- 3222 NSRIC 515-294-2739 ment to transgenic crops. Email: [email protected] Web: www.ans.iastate.edu/ Ph.D., University of Vienna, 2004 Sashital, Dipali G. Research Interests: Comparative genomics, food safety, Roy J. Carver Department of Biochemistry, Biophysics and gene expression, genomics, metagenomics, microbial Molecular Biology; Assistant Professor genetics, transcriptome analysis 3112 Molecular Biology 515-294-5121 Research Description: Interactions of the microbiome Email: [email protected] with farm animal hosts. Functional characterization of Liste- Ph.D., University of Wisconsin, 2006 ria monocytogenes in food production environments. Research Interests: Structural biology, CRISPR, RNA structure and function, protein structure and function, gene expression, nucleic acids Research Description: Study of CRISPR-Cas bacterial immune systems and biotechnology tools.

45 Faculty Profiles Schnable, Patrick S. Sebranek, Joseph G. Agronomy, C. F. Curtiss Distinguished Professor and Iowa Animal Science, Food Science and Human Nutrition Corn Promotion Board Endowed Chair; Plant Sciences University Professor Institute, Director 215 Meat Laboratory 515-294-1091 2035B Carver Co-Laboratory 515-294-0975 Fax: 515-294-5066 Fax: 515-294-5256 Email: [email protected] Email: [email protected] Web: www.ans.iastate.edu/people/joseph-sebranek Web: schnablelab.plantgenomics.iastate.edu Ph.D., University of Wisconsin, 1974 Ph.D., Iowa State University, 1986 Research Interests: Meat science and technology Research Interests: Bioinformatics, computational biology, Research Description: Investigation of various meat and genome mapping, genomics, molecular genetics of plants, non-meat ingredients for effects on product quality and plant breeding, plant genetics, Big Data, phenomics. safety. Physical, chemical and microbiological implications of ingredient applications are studied.

Schneider, Ian C. Chemical and Biological Engineering, Associate Professor Selsby, Joshua 2114 Sweeney Hall 515-294-0450 Animal Science, Associate Professor Fax: 515-294-2689 2356 Kildee Hall 515-294-7227 Email: [email protected] Fax: 515-294-4471 Web: www.cbe.iastate.edu/ians/ Email: [email protected] Ph.D., North Carolina State University, 2005 Web: www.ans.iastate.edu/people/joshua-selsby Research Interests: Biomedical engineering, biomaterials, Ph.D., University of Florida, 2005 cancer, cell biology, live-cell imaging, microfluidics, signal Research Interests: Animal physiology, cell biology, gene transduction, tissue engineering expression, human nutrition and health, human physiology, Research Description: Engineering environments to study muscle biology, proteomics, stem cells, autophagy and control migration, particularly contact guidance, Research Description: Muscle injury and disease. Coun- chemotaxis and durotaxis. Mechanical regulation of extra- termeasures and therapies using conventional and gene cellular matrix remodeling and degradation. Regulation of transfer approaches. Development, validation, and use of matrix metalloproteinases and paracrine signaling. large animal models of muscle diseases. Heat stress-mediated changes to skeletal muscle physiology.

Scott, Paul Agronomy, Associate Professor; USDA, Collaborator Serão, Nick, VL 1407 Agronomy Hall 515-294-7825 Animal Science, Assistant Professor Fax: 515-294-9359 225A Kildee Hall 515-294-3435 Email: [email protected] Email: [email protected] Web: www.agron.iastate.edu/people/marvin-scott Web: faculty.sites.iastate.edu/serao/ Ph.D., Purdue University, 1992 Ph.D., University of Illinois at Urbana-Champaign, 2012 Research Interests: Gene expression, molecular Research Interests: Quantitative genetics and genomics, genetics of plants, plant breeding, plant physiology, animal genetics, statistical methods, swine genetics transgenic plants Research Description: Identification of genomic regions Research Description: Molecular genetics and biochemis- associated with complex traits in pigs, such as reproduc- try of grain quality traits in maize. Biotechnology and plant tion and responses to diseases. Development of statistical breeding approaches to crop improvement. methods for genomic analyses.

Sebbag, Lionel Veterinary Clinical Sciences, Assistant Professor 1832 Veterinary Medicine 515-291-1127 Email: [email protected] Web: vetmed.iastate.edu/users/lsebbag DVM, Dipl. ACVO, National Veterinary School of Toulouse, 2011 Research Interests: Ocular pharmacology, translational models of ocular diseases Research Description: Tear film dynamics and pharmacology in health and disease.

46 Research in Biotechnology 2020 Serb, Jeanne Shanks, Jacqueline V. Office of Biotechnology, Director; Ecology, Evolution and Chemical and Biological Engineering Organismal Biology, Associate Professor Manley R. Hoppe Professor 313 Bessey Hall 515-294-7479 3031 Sweeney Hall 515-294-4828 Fax: 515-294-1337 Fax: 515-294-2689 Email: [email protected] Email: [email protected] Web: www.eeob.iastate.edu/people/jeanne-serb Web: www.engineering.iastate.edu/directory/?user_ Ph.D., University of Alabama, 2003 page=jshanks Research Interests: Molecular genetics of animals, Ph.D., California Institute of Technology, 1989 comparative genomics, developmental biology, gene Research Interests: Biochemical engineering, metabolic expression, molecular evolution, animal cell culture, engineering, natural products, plant cell culture, systems bioinformatics, neuroscience, transcriptome analysis, biology, metabolomics protein structure and function Research Description: Metabolic network analysis of me- Research Description: Molecular mechanisms involved tabolism in plants and microbes. Development of analytical in eye development, phototransduction, and structural tools, including 2D NMR, HPLC and MS, to obtain fluxes in change over time, molecular evolution, G-protein coupled plant tissues and microbes. Applications to metabolic engi- receptions, opsin, evolution of developmental pathways, neering of indole alkaloids, protein and oil synthesis in crop identification of genetic and environmental factors involved plants, and biorenewable chemicals from microbes. in complex trait evolution with an emphasis on eyes, phylogenetics. Shao, Zengyi Chemical and Biological Engineering, Assistant Professor Severin, Andrew J. 4140 Biorenewables Research Laboratory 515-294-1132 Ecology, Evolution and Organismal Biology, Adjunct Email: [email protected] Assistant Professor; Office of Biotechnology Genome Ph.D., University of Illinois at Urbana Champaign, 2009 Informatics Facility, Manager Research Interests: Synthetic biology, biochemical en- Manager gineering, biorenewables, metabolic engineering, natural 451 Bessey Hall (515) 294-1320 products, protein engineering Email: [email protected] Research Description: Elucidating the functional genom- Web: gif.biotech.iastate.edu ics of high-performing microbial species that have unique Ph.D., Iowa State University, 2009 biochemical, metabolic, and physiological features. Devel- Research Interests: Bioinformatics, Big Data, aquaculture, oping platform technologies to provide generalizable strain- comparative genomics, computational biology, evolutionary engineering solutions, enabling rapid-functional modifica- biology, functional genomics, gene expression, genome tions of high-performance microbes, and expanding the mapping, genomics, metagenomics, proteomics, microbial current collection of microbial factories. genetics, plant genetics, protein structure and function Research Description: Utilization of high-throughput sequencing technology to improve genomic resources for Sharp, Rick L. sustainable agriculture, aquaculture and livestock. Kinesiology, Professor Exploration of these data to improve understanding of 250 Forker Building 515-294-8650 genome evolution as it relates to the phenotype. Fax: 515-294-8740 Email: [email protected] Ph.D., Ball State University, 1983 Shanks, Brent Research Interests: Carbohydrates, human nutrition and Chemical and Biological Engineering, Steffenson and health, metabolic engineering, muscle biology Distinguished Professor; NSF Engineering Research Center Research Description: Human muscle metabolism during for Biorenewable Chemicals, Director exercise, role of nutrient availability, and factors influencing 1140L Biorenewable Research Laboratory 515-294-1895 human health and performance. Muscle function in aging Fax: 515-294-2689 and countermeasures in minimizing muscle loss. Email: [email protected] Web: www.cbe.iastate.edu/the-department/ facultystaff/?user_page=bshanks Ph.D., California Institute of Technology, 1988 Research Interests: Biorenewables, catalysis, nanotechnology Research Description: Rational design of catalysts for the conversion of biorenewables to chemicals and fuels. Design and synthesis of molecular reaction domains in solid catalysts. 47 Faculty Profiles Shelley, Mack C. Shrotriya, Pranav Political Science and Statistics; University Professor Mechanical Engineering, Associate Professor 509 Ross Hall 515-294-1075 2025 Black Engineering Building 515-294-9719 Fax: 515-294-1003 Fax: 515-294-3261 Email: [email protected] Email: [email protected] Web: www.pols.iastate.edu/faculty/mack-shelley/ Ph.D., University of Illinois, Urbana-Champaign, 2001 Ph.D., University of Wisconsin-Madison, 1977 Research Interests: Nucleic acids, drug detection, Research Interests: Public policy, bioethics, economic nanotechnology implications, food safety, risk assessment, sociopolitical Research Description: Sensors for chemical and biological implications, nutrition species detection, investigation of the mechanical loading Research Description: Attitudes and behavior of corpo- influence in corrosion of biomedical implants, mechanics rate, farm operator, academic, and political participants in governing biomolecular binding and separation, investiga- biotechnology. Issues include government research policy, tion of surface stress development due to chemical binding university-industry linkages, conflicts of interest, university and interface reactions. research policy, and commercialization.

Singh, Asheesh K. Shirtcliff, Elizabeth (Birdie) Agronomy, Assistant Professor Human Development Family Studies, Professor 1501 Agronomy Hall 515-294-3268 2361C Palmer Building NA Email: [email protected] Email: [email protected] Ph.D., University of Guelph, 2007 Web: www.hdfs.hs.iastate.edu/directory/profile. Research Interests: Genomics, molecular genetics of php?u=birdie&embedded=true plants, phenomics, plant breeding, stress response in Ph.D., Penn State, 2003 plants Research Interests: Biostatistics, brain research, devel- Research Description: Soybean breeding, unraveling the opmental biology, evolutionary biology, human physiology, genetic architecture of important traits using genome-wide neuroscience association and epistaxis studies, genome-wide prediction, Research Description: Biobehavioral mechanisms that discovery and application of molecular markers in soybean illustrate the profound impact that a child’s early environ- breeding. ment exerts on their physiology. The Stress Physiology Investigative Team (SPIT) Laboratory collects biomarkers noninvasively in humans. Examination of stress-biomarkers Singh, Ravindra like cortisol, bonding biomarkers like oxytocin, develop- Biomedical Sciences, Professor ment biomarkers like testosterone, or immune biomarkers 2034 Veterinary Medicine 515-294-8505 like herpes simplex virus. Ultimately, the goal is to use Fax: 515-294-2315 biomarkers to point to what really matters: the child’s Email: [email protected] family and social environment. Ph.D., Russian Academy of Sciences, 1993 Research Interests: Biomedical engineering, cell biology, gene expression, drug detection, molecular evolution, Shogren-Knaak, Michael molecular genetics of animals, neuroscience, neurotoxi- Roy J. Carver Department of Biochemistry, Biophysics and cology, nucleic acids, protein structure and function, RNA Molecular Biology; Associate Professor structure and function, stress response in animals 4214 Molecular Biology Building 515-294-9015 Research Description: Mechanism of alternative splicing, Fax: 515-294-0453 a process that increases the coding potential of genomes Email: [email protected] in higher eukaryotes. Understanding the molecular basis of Ph.D., California Institute of Technology, 2000 alternative splicing in human and animal diseases, including Research Interests: DNA structure and function, enzyme neurological and neuromuscular disorders, cardiovascular mechanisms, gene expression, protein structure and disorders and cancer. RNA-protein interactions and isola- function, structural biology tion of RNA aptamers as detection and diagnostic tools. Research Description: The effects of post-translational histone modifications on chromatin structure and function. Special emphasis on the establishment and role of lysine acetylation and methylation.

48 Research in Biotechnology 2020 Sivasankar, Sanjeevi Research Description: Development of information- Physics and Astronomy, Associate Professor theoretical and algebraic techniques for the modeling of A319 Zaffarano Hall 515-233-1220 complex systems and biological processes. Applications Fax: 515-294-6027 include demography, ecology, speciation, oncogenesis, Email: [email protected] senescence, and gene expression. Web: sivasankar.physics.iastate.edu Ph.D., University of Illinois at Urbana Champaign, 2001 Research Interests: Biomaterials, biomedical engineering, Smith, Ryan C. cancer, carbohydrates, cell biology, imaging technology, Entomology, Assistant Professor nanotechnology, protein structure and function, structural 442 Science II 515-294-8828 biology Email: [email protected] Research Description: Single molecule biophysics, cell Ph.D., University of California, Riverside, 2007 adhesion biophysics, structure-function relationship of bio- Research Interests: Genomics, infectious diseases, molecules, development of novel single molecule technolo- immunobiology, proteomics, vector-borne disease, malaria, gies for biological research, fluorescence resonance energy arboviruses transfer (FRET), atomic force microscopy. Research Description: Host-pathogen interactions between vector-borne diseases and their mosquito hosts. Address fundamental questions using interdisciplinary Smith, Emily techniques in molecular and cell biology, including Chemistry, Professor genomics and proteomics technologies. Mosquito innate 0706 Gilman Hall 515-294-1424 immune system. Fax: 515-294-0105 Email: [email protected] Web: esmithgroup.wixsite.com/website Song, Xueyu Ph.D., University of Wisconsin, 2003 Chemistry, Professor Research Interests: Imaging technologies, nanotechnol- 303C Wilhelm Hall 515-294-4383 ogy, biophysics, cell culture, data science Fax: 515-294-0105 Research Description: Vibrational and fluorescence imag- Email: [email protected] ing of cellular processes in cell culture and tissue with an Web: www.public.iastate.edu/~xsong/ emphasis on protein-protein and protein-ligand interactions Ph.D., California Institute of Technology, 1995 in the cell membrane. Novel instrument development for Research Interests: Computational biology, photosynthe- biological research. sis and photorespiration, protein structure and function Research Description: Electron transfer and energy transfer in photosynthesis. Dielectric response of proteins. Smith, Jodi D. Protein crystallization theory. Veterinary Pathology, Assistant Professor 2714 Veterinary Medicine 515-294-9013 Fax: 515-294-5423 Soupir, Michelle Email: [email protected] Agricultural and Biosystems Engineering DVM, Ph.D., Dipl. ACVP, Iowa State University, 2004, 2008, Associate Professor 2010 3358 Elings Hall 515-294-2307 Research Interests: Infectious diseases, comparative Fax: 515-294-2255 pathology, neuroscience Email: [email protected] Research Description: Investigation of pathogenic Web: www.public.iastate.edu/~msoupir/ mechanisms of disease in neurodegenerative protein Ph.D., Virginia Polytechnic Institute and State University, aggregation disorders. 2008 Research Interests: Antimicrobials, epidemiology, waste management Smith, Jonathan D.H. Research Description: Soil and water quality, nonpoint Mathematics, Professor source pollution control, watershed management and 496 Carver Hall 515-294-8172 water quality monitoring. Lab and field scale studies Fax: 515-294-5454 examining the occurrence, fate and transport of patho- Email: [email protected] gens, pathogen indicators and contaminants of emerging Web: orion.math.iastate.edu/jdhsmith/ environmental concern (CoEECs), such as antibiotics and Ph.D., University of Cambridge, 1975 antibiotic-resistant bacteria, to surface and groundwater Research Interests: Biostatistics, evolutionary biology, systems. Improvement of the Total Maximum Daily Load gene expression, senescence (TMDL) development and implementation process, impact of landuse practices on water quality, and development of management practices to reduce pollutant transport. 49 Faculty Profiles Sponseller, Brett A. Stegemoller, Elizabeth Veterinary Clinical Sciences, Assistant Professor; Kinesiology, Assistant Professor Veterinary Microbiology and Preventive Medicine 111K Forker Building 515-294-5966 Associate Professor Fax: 515-294-8740 2134 Veterinary Medicine 515-294-2237 Email: [email protected] Fax: 515-294-8500 Ph.D., Northwestern University, 2010 Email: [email protected] Research Interests: Neuroscience, Parkinson’s disease, DVM, Ph.D., Cornell University, Iowa State University, music therapy 1994, 2003 Research Description: Understanding how music influenc- Research Interests: Immunobiology, molecular virology es movement and associated neurophysiology in persons Research Description: Elucidation of viral mechanisms with Parkinson’s disease. of humoral immune evasion, with special emphasis on viruses of horses. Identification of conserved epitopes for neutralizing antibody among diverse equine infectious Steward, Brian L. anemia virus isolates. Equine neonatal immunology, with Agricultural and Biosystems Engineering, Professor emphasis on predilection to diseases caused by intracel- 2325 Elings Hall 515-294-1452 lular pathogens. Fax: 515-294-2255 Email: [email protected] Ph.D., University of Illinois, Urbana-Champaign, 1999 Stabel, Judith R. Research Interests: Imaging technology Veterinary Microbiology and Preventive Medicine Research Description: Automation and sensing technol- Professor; Animal Science, Collaborator ogy for plant production. National Animal Disease Center 515-337-7304 Fax: 515-337-7428 Email: [email protected] Sundararajan, Sriram Web: nadc.ars.usda.gov Mechanical Engineering, Professor Ph.D., North Carolina State University, 1987 4100 Marston 515-294-1050 Research Interests: Immunobiology, microbial virulence, Fax: 515-294-3261 vaccines Email: [email protected] Research Description: Understanding host immune Web: www.me.iastate.edu/directory/faculty/sriram-sunda- response and pathogenesis of infection with Mycobacte- rarajan/ rium paratuberculosis, a facultative intracellular pathogen. Ph.D., The Ohio State University, 2001 Defining molecular mechanisms of bacterial evasion from Research Interests: Biobased lubricant additives, imaging host defenses in an infective state. Application of these technology, nanotechnology studies to development of improved diagnostic tests and Research Description: Investigation of friction, lubrication vaccines for Johnne’s disease. and wear properties of materials and interfaces. Experi- mental capabilities in evaluating surface structural and mechanical properties. Stanley, Levi Chemistry, Assistant Professor 3101E Hach Hall 515-294-3609 Swanner, Elizabeth Fax: 515-294-6342 Geological and Atmospheric Sciences, Assistant Professor Email: [email protected] 354 Science I (515) 294-5826 Web: www.chem.iastate.edu/faculty/Levi_Stanley/ Email: [email protected] Ph.D., North Dakota State University, 2007 Web: geobiochem.ge-at.iastate.edu/ Research Interests: Catalysis, metalloproteins, protein Ph.D., University of Colorado - Boulder, 2011 engineering Research Interests: Microbial ecology, geochemistry Research Description: Development of a platform for the Research Description: Ascertaining the geochemical generation, expression, purification and metal substitution imprint of microbial life on Earth through geological time of libraries of mutant metal-substituted carbonic anhydras- using tools and techniques from diverse fields. es. Application of metal-substituted carbonic anhydrases as hybrid transition metal catalysts in synthetic organic chemistry. Application of modern protein engineering and directed evolution strategies to improve the activity and selectivity of metal-substituted carbonic anhydrases in oxidation and insertion reactions.

50 Research in Biotechnology 2020 Talbert, Joey Research Description: Plant molecular biology, eukaryotic Food Science and Human Nutrition, Assistant Professor gene regulation and expression, plant-insect interaction, 1547 Food Sciences Building 515-294-7015 biochemistry of nectar production. Email: [email protected] Web: talbertresearchgroup.org Ph.D., Cornell University, 2009 Toth, Amy L. Research Interests: Biosensors, food biotechnology, food Ecology, Evolution and Organismal Biology preservation and quality, food processing, food safety, Assistant Professor nanotechnology, protein engineering 251 Bessey Hall 515-294-3121 Research Description: Application of enzymes and whole Fax: 515-294-1337 cells to enable and improve the conversion, preservation, Email: [email protected] and analysis of post-harvest food and non-food biological Web: www.tothlab.org materials. Ph.D., University of Illinois, 2006 Research Interests: Comparative genomics, evolutionary biology, gene expression, molecular ecology, molecular Tessonnier, Jean-Philippe evolution, molecular genetics of animals Chemical and Biological Engineering, Assistant Professor Research Description: Molecular and physiological basis 2138 Biorenewables Research Laboratory 515-294-4595 of social behavior in bees and wasps. Comparative ge- Fax: 515-294-2689 nomics of the transition from solitary to social behavior in Email: [email protected] insects. Transcriptome and genome sequencing of emerg- Web: www.tessonniergroup.org/ ing model organisms (social bees and wasps). Epigenetics Ph.D., University of Strasbourg - France, 2005 of behavior and development in insects. Effect of nutri- Research Interests: Biorenewables, carbohydrates, tion and viruses on honey bee health, behavior and gene catalysis, nanotechnology expression. Research Description: Development of sustainable solutions for the production of chemicals and energy from renewable feedstock. Design of novel heterogeneous cata- Travesset, Alex lysts at the nanoscale. Current topics include nanocarbons Physics and Astronomy, Associate Professor and hierarchical zeolites. A503 Zaffarano Hall 515-294-7191 Fax: 515-294-6027 Email: [email protected] Thippeswamy, Thimmasettappa Web: www.public.iastate.edu/~trvsst/index.html Biomedical Sciences, Professor Ph.D., University of Barcelona, 1997 2036 Veterinary Medicine 515-294-2571 Research Interests: Biomaterials, computational biology, Email: [email protected] lipids, proteins, viruses, materials science BVSc, MVSC, Ph.D., Bangalore Veterinary College (India), Research Description: Self-assembly of biological matter. Bangalore Veterinary College (India), University of Liverpool Virus structure, spherical crystals, nanoparticle structure. (England), 1985, 1991, 1998 Research Interests: Brain research, cell biology, neuroscience, neurotoxicology, signal transduction Tsou, Jonathan Y. Research Description: Identification of molecular targets Philosophy and Religious Studies, Assistant Professor for disease modification in epilepsy and organophosphate- 402 Catt Hall 515-294-1499 induced long-term neurotoxicity. Tweaking neuron-glial Fax: 515-294-0780 communication during early exposure to neurotoxins can Email: [email protected] prevent/modify the disease onset. Web: sites.google.com/site/jytsou/ Ph.D., University of Chicago, 2008 Research Interests: Bioethics, biomedical engineering, Thornburg, Robert W. human physiology, neuroscience, diagnostics Roy J. Carver Department of Biochemistry, Biophysics and Research Description: The role that research on pharma- Molecular Biology; Professor cological treatments for mental disorders plays in the de- 2212 Molecular Biology Building 515-294-7885 velopment of neurobiological theories of psychopathology. Fax: 515-294-0453 Email: [email protected] Ph.D., University of South Carolina, 1981 Research Interests: Molecular genetics of plants, nucleic acids, plant cell culture, plant hormone synthesis and action, plant-pest interactions, stress response in plants

51 Faculty Profiles Tuggle, Christopher K. post-synaptic density (PSD) signaling complexes using Animal Science, Professor hydrogen/deuterium exchange mass spectrometry. 2255 Kildee Hall, 806 Stange Road 515-294-4252 Fax: 515-294-2401 Email: [email protected] Valentine, Rudy Web: www.ans.iastate.edu/people/christopher-tuggle Kinesiology, Assistant Professor Ph.D., University of Minnesota, 1986 243 Forker Building 515-294-3867 Research Interests: Animal genetics, animal protection Email: [email protected] from infectious diseases, bioinformatics, comparative Ph.D., University of Illinois, 2010 genomics, gene expression, genomics, immunobiology, Research Interests: Animal cell culture, human nutrition molecular genetics of animals, systems biology and health, human physiology, immunobiology, muscle Research Description: Gene expression and genome biology analysis in animals, emphasizing transcriptomics and epi- Research Description: Identifying the mechanism(s) genetics in tissues affecting disease resistance. through which behavioral (e.g., exercise and diet) and Developing immune-deficient pigs as large-animal models pharmacologic therapies mitigate cardiometabolic disease for regenerative medicine and cancer research. pathogenesis, with a focus on inflammation. Examining the relationships between physical activity/fitness, diet, and risk factors for physical disability (e.g., muscle strength, Tylka, Gregory L. muscle quality, and physical function). Investigating the Plant Pathology and Microbiology, Professor biological and behavioral risk factors for obesity-associated 2207 Advanced Teaching and Research Building comorbidities. 515-294-3021 Fax: 515-294-3851 Email: [email protected] Valenzuela, Nicole Web: www.tylkalab.org Ecology, Evolution and Organismal Biology Ph.D., University of Georgia, 1990 Associate Professor Research Interests: Nematode behavior and chemical 337 Bessey Hall 515-294-1285 ecology, integrated pest management, natural products, Fax: 515-294-1337 plant-pest interactions, soil-plant interactions, microfluidics Email: [email protected] Research Description: Investigation of the biology and Web: www.eeob.iastate.edu/faculty/valenzuela/ management of the soybean cyst nematode (SCN), Ph.D., State University of New York, Stony Brook, 1999 Heterodera glycines, including determining the relation- Research Interests: Animal genetics, comparative ship between reproduction of SCN on resistant soybean genomics, chromosome evolution, developmental biology, varieties and agronomic performance and season-long epigenetics, evolutionary biology, gene expression, nematode reproduction in the field, the biological basis for genome mapping, molecular evolution, molecular genetics the increase in sudden death syndrome (SDS) disease in of animals, animal embryos soybeans co-infected with soybean cyst nematode and Research Description: Ecological and evolutionary the biological and biochemical aspects of the interaction of genomics of phenotypic plasticity, developmental SCN with the soybean aphid, Aphis glycines. Other re- pathways, chromosome evolution, and sex determination search includes designing and creating small-scale micro- of reptiles. Life history evolution, conservation and scope chip apparatus and methods for high-throughput, ecological genetics of turtles. sensitive, unbiased assessment of effects of potential nematode management compounds on specific life stages and processes in the plant-parasitic nematode life cycles. VanVeller, Brett Chemistry, Assistant Professor 3126 Hach Hall 515-294-7613 Underbakke, Eric S. Email: [email protected] Roy J. Carver Department of Biochemistry, Biophysics and Web: vanvellergroup.chem.iastate.edu Molecular Biology; Assistant Professor Ph.D., MIT, 2011 4265 Molecular Biology Building 515-294-5793 Research Interests: Cancer, catalysis, DNA structure and Email: [email protected] function, imaging technology, structural biology Ph.D., University of Wisconsin, 2008 Research Description: Employ chemistry-based insights Research Interests: Protein structure and function, struc- to investigate the widening interface of chemistry and tural biology, signal transduction biology. Develop tools and biomimetic materials to interro- Research Description: Applying mass spectrometry- gate, understand, and manipulate the interactions between based protein footprinting strategies to map protein-protein biological building blocks. Synthetic chemistry and the interactions in scaffolded signaling complexes. Probing development of new methods. Applying the principles of conformational changes and dynamic rearrangements in chemistry towards precise molecular-level design.

52 Research in Biotechnology 2020 Vaughn, Eric M. formance, and interpretation of electrophysiology Veterinary Microbiology and Preventive Medicine experiments. Assistant Professor 2412 South Loop Drive 515-268-7425 Fax: 515-268-7556 Vigil, Dennis R. Email: [email protected] Chemical and Biological Engineering, Associate Professor Ph.D., Iowa State University, 1994 3037 Sweeney Hall 515-294-6438 Research Interests: Molecular virology, vaccines, animal Fax: 515-294-2689 protection from infectious diseases, antiviral agents, Email: [email protected] microbial genetics, immunobiology, microbial virulence, Web: www.engineering.iastate.edu/directory/?user_ molecular genetics of microorganisms page=vigil Research Description: Characterization of infectious Ph.D., University of Michigan, 1990 agents and key immunogens associated with clinical Research Interests: Biochemical engineering, bioreactors, disease in animals. Protein expression and structure-based biorenewables, energy development vaccine design. Development of novel bacterial and viral Research Description: Development and scale up of novel vectors as vaccines. NextGen technologies and genomics. algal photobioreactors and photobioreactor-separators. Special emphasis on use of computational fluid dynamics simulations and advanced radiation transport models to Venditti, Vincenzo understand the role of light on biomass productivity. Chemistry, Assistant Professor 0219 Hach Hall 515-294-1044 Email: [email protected] Vollbrecht, Erik Ph.D., Universita’ degli Studi di Siena, 2009 Genetics, Development and Cell Biology; Professor Research Interests: Metabolic engineering, metallopro- 2206 Molecular Biology 515-294-9009 teins, protein structure and function, structural biology Fax: 515-294-7629 Research Description: Investigation of structure/dynam- Email: [email protected] ics/function relationship in enzymatic proteins by NMR and Web: www.gdcb.iastate.edu/people/erik-vollbrecht other biophysical and computational methods. Ph.D., University of California, Berkeley, 1997 Research Interests: Genomics, developmental biology, molecular evolution, plant genetics, computational biology, Verhoeven, David molecular genetics of plants, stress response in plants, VMPM, Assistant Professor systems biology, transposable elements 2152 Veterinary Medicine 515-294-2562 Research Description: Elucidate molecular mechanisms Email: [email protected] of plant architecture and reproduction by genetics and Ph.D., Iowa State University, 2007 genomics. Characterize genes controlling tassel and ear Research Interests: Infectious diseases, immunobiology, development in maize. Dissect the interface between plant microbial virulence, molecular virology, virology development and environmental stresses. Research Description: Identification of divergent/conver- gent immune responses between infants and adults to bacterial and viral pathogens. Respiratory bacterial and viral Walley, Justin pathogenesis. Bacterial vaccines for respiratory pathogens. Plant Pathology and Microbiology, Assistant Professor CD4 T-cell immunopathology during RSV and Influenza 423 Bessey Hall 515-294-6969 infections. Mucosal immunology. Email: [email protected] Ph.D., University of California, Davis, 2009 Research Interests: Bioinformatics, plant-pest interactions, Verma, Saurabh proteomics, stress response in plants, systems biology Biomedical Sciences, Research Assistant Professor Research Description: Gaining an understanding of the 2030 Vet Med 515-294-2547 molecular basis of plant immunity using systems biology Email: [email protected] approaches to analyze transcriptomic and proteomic data. DVM, Ph.D., Iowa State University, 2008 Research Interests: Brain research, infectious diseases, molecular pharmacology, neuroscience, toxicology Research Description: Nicotinic acetylcholine receptors in filarial nematode, Brugia malayi, and heartworm (Dirofilaria immitis). The current project involves the identification and pharmacological characterization of cholinergic receptors and their expression in Xenopus oocytes to elucidate their structure. Independently responsible for the planning, per-

53 Faculty Profiles Wang, Chong Wang, Xuefeng Veterinary Diagnostic and Production Animal Medicine Physics and Astronomy, Assistant Professor Associate Professor A305 Zaffarano Physics Addition 515-294-3545 2239 Lloyd Veterinary Medical Center 515-294-3836 Email: [email protected] Fax: 515-294-1072 Web: mcm.physics.iastate.edu Email: [email protected] Ph.D., Iowa State University, 2009 Ph.D., Cornell University, 2006 Research Interests: Animal cell culture, biomaterials, Research Interests: Antimicrobials, Big Data, microbi- biosensors, cell adhesion, cell mechanobiology, omes, statistics, bioinformatics, biostatistics, gene expres- microfluidics, platelet contraction, single molecule imaging. sion, genomics, proteomics, quantitative genetics Research Description: Studying cell mechanics using Research Description: Statistical methods and theories single molecule approaches. Investigating cell adhesion, and their applications to biological and medical sciences. spreading, migration and endocytosis with molecular force sensor and single molecule imaging microscopy.

Wang, Kan Agronomy, Professor Wang, Yu G405 Agronomy Hall 515-294-4429 Political Science, Assistant Professor Fax: 515-294-2299 545 Ross Hall 515-294-3934 Email: [email protected] Email: [email protected] Web: www.agron.iastate.edu/people/kan-wang Ph.D., Georgia Institute of Technology, 2014 Ph.D., University of Ghent, Belgium, 1987 Research Interests: Biorenewables, energy development, Research Interests: Cell biology, plant cell culture, plant sociopolitical implications, economic implications genetic transformation, genome editing, transgenic plants Research Description: Policy and economic analysis of re- Research Description: Exploring novel technologies for newable energy, biorenewables, and energy development. efficient genetic transformation genome-editing in plants. Assessing the specificity of CRISPR system for increasing editing efficiency and the potential for unanticipated Wannemuehler, Michael effects from off-target mutations. Studying the functions of Veterinary Microbiology and Preventive Medicine non-coding small RNAs of Agrobacterium tumefaciens to Professor improve its ability as a tool for plant genetic transformation. 2178 Veterinary Medicine 515-294-3534 Fax: 515-294-8500 Email: [email protected] Wang, Qun Ph.D., University of Louisville, 1981 Chemical and Biological Engineering, Assistant Professor Research Interests: Animal protection from infectious 1014 Sweeney Hall 515-294-4218 diseases, host-microbe interaction, human protection from Email: [email protected] infectious disease, immunobiology, vaccines Web: www.qunwanglab.com Research Description: Elucidating the impact of intesti- Ph.D., University of Kansas, 2010 nal microbiota on mucosal immune mechanisms. Use of Research Interests: Biomaterials, drug detection, microarray techniques to evaluate changes in host gene nanotechnology, intestinal stem cells, stem cells expression following intestinal colonization with novel Research Description: Using Biomaterials, Intestinal bacteria. Use of gnotobiotic mice to evaluate interactions Engineering, Nanotechnology and Drug Delivery (BIND), between members of intestinal microbiota. Development to provide innovative solutions and products for human of immunotherapeutic approaches, including novel health. Pursuing better disease diagnosis and effective botanical extracts to regulate intestinal inflammation therapeutics. Coordinating the latest science in materials associated with colitis. Development and evaluation of science, stem cells, microfabrication, and pharmaceutical protective immunity using novel polymers and nanotech- chemistry to provide new tools for innovative solutions in a nology that provide the potential for development of single multidisciplinary approach. dose delivery platforms for the induction of long-lived humoral and cell-mediated immunity to bacterial and viral pathogens.

54 Research in Biotechnology 2020 Ware, Wendy molecular biology and nutritional modulation of beta-caro- Veterinary Clinical Sciences, Biomedical Sciences tene 15, 15’-monooxygenase activity. Professor 2038 Veterinary Medicine 515-294-4900 Fax: 515-294-9281 Whitham, Steve Email: [email protected] Plant Pathology and Microbiology, Associate Professor DVM, The Ohio State University, 1982 4203 Advanced Teaching and Research Building Research Interests: Animal physiology 515-294-4952 Research Description: Study of heart failure and Fax: 515-294-9420 myocardial diseases. Animal physiology. Email: [email protected] Ph.D., University of California, Berkeley, 1995 Research Interests: Gene editing, gene expression, Watrelot, Aude genomics, molecular genetics of plants, molecular virology, Food Science and Human Nutrition, Assistant Professor plant genetics, plant-pest interactions, plant physiology, 2567 Food Sciences Building, 536 Farm House Lane signal transduction, stress response in plants, systems 515-294-0343 biology, functional genomics Email: [email protected] Research Description: Identification of pathogen effec- PhD, University of Avignon, France, 2013 tors and their host targets that modulate pathogenesis and Research Interests: Food and wine polyphenols and interactions of plants with multiple stress agents, including polysaccharides characterization, metabolites from plant viruses, fungi, and insects. Development of virus-based material, analytical chemistry, structure-activity relationship tools for functional genomics studies in crop plants, and of polyphenols, thermodynamic parameters of interactions application of these tools to understand plant immune between macromolecules (polyphenols, polysaccharides, signaling networks. proteins), chemistry and sensory analysis identification of the astringency mechanism and perception Research Description: Understanding the relationship Willette, Auriel between food and wine macromolecules, such as poly- Food Science and Human Nutrition, Assistant Professor phenols and polysaccharides and their interactions with 1109 HNSB 515-294-3110 other compounds that might affect their extraction from a Email: [email protected] plant material and their reactivity, especially the astringency Ph.D., MS, University of Wisconsin-Madison, 2010 mechanism. Research Interests: Biostatistics, human nutrition and health, immunobiology, neuroscience, randomized clinical trials Wendel, Jonathan F. Research Description: Immunologic and endocrine bio- Ecology, Evolution and Organismal Biology markers of brain activity, neural structure and composition, Distinguished Professor and Chair and the etiopathogenesis of neurodegenerative disorders. 345A Bessey Hall 515-294-7172 Fax: 515-294-1337 Email: [email protected] Wilson, Lester A. Web: www.eeob.iastate.edu/people/jonathan-wendel Food Science and Human Nutrition, University Professor Ph.D, University of North Carolina, 1983 2541 Food Sciences Building 515-294-3889 Research Interests: Plant evolutionary genomics Fax: 515-294-8181 Research Description: Genetic aspects of evolutionary Email: [email protected] divergence between populations and species, molecular Ph.D., University of California, 1975 evolution and evolution of cultivated plants and their wild Research Interests: Food preservation and quality, food relatives. processing, plant protein and oil utilization, food safety Research Description: Influence of biotechnology and processing and storage conditions on the chemical compo- White, Wendy S. sition, flavor chemistry and quality of soy foods and spices. Food Science and Human Nutrition, Associate Professor 1111 Human Nutritional Sciences 515-294-3447 Fax: 515-294-6193 Email: [email protected] Ph.D., Cornell University, 1990 Research Interests: Human nutrition and health, lipids, vitamins and minerals Research Description: Bioavailability, health benefits and metabolism of beta-carotene and other carotenoids,

55 Faculty Profiles Wise, Roger P. Wu, Yue Plant Pathology and Microbiology, Professor Chemical and Biological Engineering, Associate Professor USDA-ARS, Affiliate Professor 2033 Sweeney 617-875-0178 411 Bessey Hall 515-294-9756 Email: [email protected] Fax: 515-294-9420 Web: www.cbe.iastate.edu/the-department/facultystaff/ Email: [email protected] profile/yuewu Web: wiselab.org Ph.D., Harvard University, 2006 Ph.D., Michigan State University, 1983 Research Interests: Animal cell culture, brain research, Research Interests: Gene expression, genome mapping, muscle biology, nanotechnology, neuroscience plant genetics, functional genomics, plant-pest interactions, Research Description: Investigation of broadly-defined STEM education nanostructured materials. Design, synthesis, characteriza- Research Description: Disease resistance signaling in tion and assembly of nanostructured materials, elucidation cereal crops. of the fundamental electronic, optical and other physical properties of these materials, and exploration of new science and applications towards the efficient harvest, Wolc, Anna storage, and conversion of solar and thermal energy. Animal Science, Adjunct Assistant Professor Seeking collaborators in animal or plant sciences to work 239E Kildee Hall on using nanowires as a platform to study the integration Email: [email protected] of nanomaterials and nanoscale energy devices into living Web: www.ans.iastate.edu/people/anna-wolc cells to create multi-functional living hybrid systems. Ph.D., Poznan University of Life Sciences, 2006 Research Interests: Animal breeding, animal genetics, genomics, quantitative genetics Wu, Zhijun Research Description: Genomic selection in poultry. QTL Mathematics, Associate Professor mapping for quantitative and threshold traits. Pedigree and 482 Carver Hall 515-294-8165 genomic analysis of longitudinal traits in livestock. Fax: 515-294-5454 Email: [email protected] Ph.D., Rice University, 1991 Wolf, Clark Research Interests: Bioinformatics, computational biology, Philosophy and Religious Studies, Professor DNA structure and function, protein structure and function, Bioethics Program, Director RNA structure and function, structural biology 435 Catt Hall 515-294-3068 Research Description: Numerical methods for structure Fax: 515-294-0780 and dynamics of locally genetically mutated proteins. Email: [email protected] Distance geometry approach to protein structure deter- Ph.D., University of Arizona, 1993 mination. Entropy maximization for phase determination Research Interests: Bioethics, sustainable agriculture, in protein X-ray crystallography. Algorithms for sequence public policy and political theory, sociopolitical implications analysis and restriction mapping. of technology. Research Description: Biotechnology and public policy, sustainability and intergenerational justice, moral, legal and Wu, Zuowei political implications of biotechnology, regulation of scien- Veterinary Microbiology and Preventive Medicine, tific research, intellectual property. Assistant Professor 515-294-6170 Email: [email protected] Wright, David A. Research Interests: Antibiotic resistance; bacterial patho- Plant Transformation Facility, Manager genesis, population genomics and evolution, bioinformatics 2519 Agronomy Hall 515-294-5415 Fax: 515-294-2299 Email: [email protected] MS, Ph.D., Iowa State University, 1997, 2000 Research Interests: Plant transformation, genome editing, zinc finger nuclease, TALEN, CRISPR/Cas9, small molecule activated genetic switch Research Description: Gene-editing technology and artificial transcription factors. Small molecule-activated genetic switches, which may be used to precisely control gene editing and transcription factor activity for use in plant transformation.

56 Research in Biotechnology 2020 Wurtele, Eve Syrkin Yin, Yanhai Genetics, Development and Cell Biology, Professor Genetics, Development and Cell Biology, Professor 538 Science I 515-294-8989 1035D Roy J. Carver Co-laboratory 515-294-4816 Fax: 515-294-1337 Email: [email protected] Email: [email protected] Web: www.gdcb.iastate.edu/people/yanhai-yin Web: www.gdcb.iastate.edu/people/eve-syrkin-wurtele Ph.D., The Scripps Research Institute, 1997 Ph.D., University of California, Los Angeles, 1980 Research Interests: Plant genetics, plant hormone Research Interests: Systems biology, bioinformatics, gene synthesis and action, signal transduction expression, lipids, metabolic engineering, orphan genes, Research Description: Regulation of plant growth, devel- plant protein and oil utilization, protein structure and opment and stress responses by plant steroid hormone function, seed physiology, stress response in plants, Brassinosteroid in Arabidopsis and maize using genetics, biorenewables, STEM education genomics and predicative phenomics approaches. Long- Research Description: Origins, functions and fates of term goal: manipulate crops for better quality, higher yield orphan genes. Metabolic networking and its regulation. and improved tolerance to stresses. Development of computational approaches to understanding big/complex biological data. Meta!Blast, the 3D interactive computer game for STEM can be downloaded Yoon, Kyoung-Jin at www.metablast.org/. Veterinary Diagnostic and Production Animal Medicine, Professor and Head of Virology/Molecular Microbiology 209 Veterinary Medical Research Institute Building 1 Yan, Jue 515-294-1083 Mathematics, Associate Professor Fax: 515-294-6619 484 Carver Hall 515-294-8166 Email: [email protected] Fax: 515-294-5454 Web: vetmed.iastate.edu/users/kyoon Email: [email protected] DVM, MS, Ph.D., Seoul National University, Iowa State Ph.D., Brown University, 2002 University, 1985, 1987, 1995 Research Interests: Animal embryos, cell biology, Research Interests: Animal cell culture, animal protection evolutionary biology, infectious diseases, immunobiology from infectious diseases, computational biology, epidemiology, immunobiology, microbial identification, microbial virulence, molecular evolution, molecular virology, Yandeau-Nelson, Marna monoclonal antibodies, vaccines, virology, diagnostics Genetics, Development and Cell Biology Research Description: Ecology of animal viral disease, Assistant Professor pathogenesis and immunobiology of animal virus infec- 4138 BRL 515-294-1079 tions with emphasis on the identification of viral virulence Email: [email protected] factors, immune-mediated or related pathogenic factors Web: www.gdcb.iastate.edu/people/marna-yandeau-nelson and interaction among different microbial agents in disease Ph.D., Iowa State University, 2005 process. Advanced and/or molecular biological techniques Research Interests: Biorenewables, functional genomics, in veterinary diagnostics. Development of prevention and/ lipids, molecular genetics of plants, plant genetics or control strategies for infectious disease, including vac- Research Description: Dissection of biosynthetic and cine development. regulatory genetic networks of metabolic traits to increase the fundamental knowledge of cellular metabolism and to use that knowledge for practical applications (i.e. plant Youngs, Curt breeding for resistance to stresses and the development of Animal Science, Professor biorenewable chemicals and fuels). 2356B Kildee Hall 515-294-5541 Fax: 515-294-4471 Email: [email protected] Yang, X. B. Web: www.ans.iastate.edu/people/curtis-youngs Plant Pathology and Microbiology, Professor Ph.D., University of Minnesota, 1985 351 Bessey Hall 515-294-8826 Research Interests: Animal embryos, animal physiology, Fax: 515-294-9420 animal reproductive efficiency, developmental biology Email: [email protected] Research Description: Manipulation of embryonic devel- Ph.D., Louisiana State University, 1989 opment to improve reproductive efficiency in farm animal Research Interests: Integrated pest management species. Development of techniques for in vitro fertiliza- Research Description: Assessment of benefit and risk tion; cryopreservation; and sexing of sheep, cattle, pigs and in association of application of biotechnology products for goat embryos. Application of embryo transfer to genetic disease control and management. Use of molecular tools improvement programs in farm animal species. for plant pathogen identification.

57 Faculty Profiles Yu, Chenxu Research Description: Plant cell wall characterization and Agricultural and Biosystems Engineering modification; analytical analysis of carbohydrate structures; Assistant Professor identification of enzymes involved in polysaccharide bio- 3224 NSRIC 515-294-4554 synthesis; genetic modification of plants using the genes Fax: 515-294-4250 involved in biosynthesis or biodegradation of cell wall poly- Email: [email protected] saccharides; and functional characterization of bioactive Ph.D., University of Wisconsin, 2003 oligosaccharides using proteomic, genetic and biochemical Research Interests: Nanotechnology, biological compound approaches. separation and detection, biosecurity, fermentation, food safety, food biotechnology, microbial identification, signal transduction, systems biology Zellner, Eric Research Description: Development of spectroscopic Veterinary Clinical Sciences, Assistant Professor imaging techniques that allow real-time monitoring of in- 2567 Lloyd Veterinary Medical Center 515-291-5076 tracellular chemical environments and molecular activities. Email: [email protected] Application of nano biosensors for pathogenic virus and/ Ph.D., Michigan State, 2008 or microorganism detection and identification in biological Research Interests: Skin, tension, tendon, suture samples, food and water. Application of nanomaterials/ Research Description: Primary interest is measuring soft nanostructures for enzymatic fermentation of soybean tissue biomechanical properties. to enrich biologically active compounds and downstream separation, extraction and purification of the products. Zhang, Jianqiang Veterinary Diagnostic and Production Animal Medicine Yu, Jianming Associate Professor Agronomy 1577 Veterinary Medicine Building 515-294-8024 1569 Agronomy Hall 515-294-2757 Fax: 515-294-3564 Email: [email protected] Email: [email protected] Ph.D., University of Minnesota, 2003 Ph.D., University of Kentucky, 2005 Research Interests: Complex trait dissection, breeding Research Interests: Virology, molecular virology, vaccines, methods diagnostics, molecular epidemiology Research Description: Developing and implementing new Research Description: Development of virological and strategies and methods in complex trait dissection and molecular diagnostic assays, investigational disease crop improvement. Genome-Wide Association Studies outbreaks and molecular characterization of animal viruses, (GWAS) with diverse germplasm or multiple designed map- virus-host cell interactions, virus pathogenesis and vaccine ping populations (such as Nested Association Mapping, development. NAM; or meta-QTL analysis), Genomic Selection (GS; or Genome-Wide Selection GWS) to efficiently integrate high throughput genotyping into various breeding processes, gene cloning for traits with agronomic and domestica- Zhang, Qijing Veterinary Microbiology and Preventive Medicine tion importance, Genotype-by-Environment Interaction Clarence Hartley Covault Distinguished Professor (GEI) and epistasis dissection to causal polymorphic sites, 1116 Veterinary Medicine 515-294-2038 genome and chromosome size evolution across taxonomic Fax: 515-294-8500 groups, and genome-wide base composition changes and Email: [email protected] underlying principles. Web: vetmed.iastate.edu/users/zhang123 Ph.D., Iowa State University, 1994 Research Interests: Microbial genetics, food safety, Zabotina, Olga genomics, immunobiology, microbial virulence, protein Roy J. Carver Department of Biochemistry, Biophysics and structure and function, proteomics Molecular Biology; Assistant Professor Research Description: Molecular mechanisms of antimi- 3212 Molecular Biology 515-294-6125 crobial resistance in foodborne pathogens. Examination of Email: [email protected] the molecular basis of pathogen-host interaction in animals. Web: www.bbmb.iastate.edu/people/olga-zabotina Understanding the immune mechanisms against coloniza- Ph.D., Kazan Institute of Biology of Russian Academy of tion by foodborne pathogens. Development of strategies Sciences, 1987 for controlling foodborne diseases. Research Interests: Biological compound separation and detection, biorenewables, carbohydrates, cell biology, enzyme mechanisms, plant physiology, signal transduction, stress response in plants, transgenic plants, molecular genetics of plants

58 Research in Biotechnology 2020 Zheng, Mai Electrical and Computer Engineering Assistant Professor 349 Durham Hall 515-294-6285 Email: [email protected] Web: www.ece.iastate.edu/~mai/ Ph.D., The Ohio State University, 2015 Research Interests: Data storage systems, cloud computing, high-performance computing, data-intensive computing Research Description: Building robust, secure, high-performance storage systems for various data.

Zimmerman, Jeffrey J. Veterinary Diagnostic and Production Animal Medicine Professor VMRI Building One 515-294-1073 Fax: 515-294-3564 Email: [email protected] Web: vetmed.iastate.edu/users/jjzimm DVM, Ph.D., Iowa State University, 1984, 1990 Research Interests: Animal protection from infectious diseases, animal reproductive efficiency, economic implications, food safety, immunobiology, microbial virulence, vaccines, diagnostic assay development, surveillance Research Description: Epidemiology, economics and diagnosis of infectious diseases of animals with particular emphasis on diseases of swine. Development, assessment and application of biotechnologies related to disease prevention, control and diagnosis.

Services and Facilities Services and Facilities Technology Transfer, Patents and Licensing Biotechnology Services for Research For the latest information and rates, see our website at www.biotech.iastate.edu.

Technology Transfer, Patents and Licensing Iowa State University provides many resources to assist in the Specific questions about each facility should be directed to the transfer of scientific discoveries and technologies to the market- contact individual(s) for the facility as listed in this publication. place. Technology transfer resources at Iowa State include the Iowa State University Research Park and the Office of Intellectual The annual publication, Service Facilities for Biotechnology Property and Technology Transfer. Research, provides additional information about these and other facilities on campus available to on- and off-campus biotechnol- Iowa State University Research Park ogy researchers. The information is also available in the top menu Iowa State has networks in place to move research results quickly of the Office of Biotechnology website: www.biotech.iastate.edu. and smoothly from the laboratory to industry. For start-up companies, the Iowa State Research Park provides an environment in which small business entrepreneurs involved in technical Animal Gene Transfer Facility products, research and development can turn their concepts into marketable products and services. Location: 1270 Kildee Hall The Iowa State Research Park offers biotechnology research facili- Hours: By appointment, 8 a.m. to 5 p.m. weekdays ties, microelectronics, new industrial materials, analytical chemis- Contact: Jason Ross, [email protected], 515-294-8647 try, computational fluid dynamics, pharmaceuticals, biologics and 515-294-4471 (fax) more, all supported by unsurpassed communication capabilities and with experts only minutes away at Iowa State University. The facility provides a variety of services to individuals who wish to utilize transgenic animals as part of their research programs. For more information, contact the Iowa State Research Park Cor- The facility maintains equipment necessary for the creation of poration, 1805 Collaboration Place, Suite 1250, Ames, IA 50010- transgenic animals via microinjection or somatic cell nuclear 9166, telephone: 515-296-PARK, website: www.isupark.org. transfer and is available to life science researchers for technical service and for training of a limited number of individuals. All work to be performed in this facility must be scheduled with the Office of Intellectual Property and Technology Transfer manager, as the facility is not staffed full-time. The Iowa State Office of Intellectual Property and Technology Transfer (OIPTT), which works with the Iowa State Research Equipment housed in the facility includes two microinjection Foundation, solicits inventions from Iowa State’s faculty, staff and workstations, micropipette pullers, a microforge, stereo-zoom students to facilitate protection, marketing and licensing activi- microscope and CO2 incubator. Additional supporting equipment ties in the technology transfer process. OIPTT provides educa- located in nearby laboratories to which access may be granted tional services to Iowa State’s faculty, staff and students regarding includes a heated microcentrifuge, tri-gas incubators, stereo-zoom intellectual property issues and works with the Office of the Vice microscopes, inverted microscope, biosafety cabinet, osmometer, President for Research to implement intellectual property policies. pH meter, embryo freezing machine, cell fusion machine, gel electrophoresis equipment and UV transilluminator. For more information about licensing or commercial development opportunities at Iowa State, visit the OIPTT website at www. Service and user fees are determined on a case-by-case basis and techtransfer.iastate.edu. are dependent upon experimental factors. Iowa State University Biotechnology Services for Research BioCentury Research Farm Location: 1327 U Avenue, Boone Academic and Industrial Users Hours: 8 a.m. to 5 p.m. weekdays A major initiative of Iowa State University has been the Other hours by arrangement establishment and support of state-of-the-art instrumentation for Contact: Matt Darr, Director, [email protected], biotechnology research. The Iowa State University biotechnology 515-294-8545, 515-432-2701 (fax) service facilities are open to faculty and students from the univer- Andrew Suby, Manager, sity, other educational institutions and industry scientists. [email protected], 515-294-6300 Equipment Development and Sharing Web: www.biocenturyresearchfarm.iastate.edu In addition to instrumentation facilities, the university has in place an organization whose primary function is the support of Iowa State University’s BioCentury Research Farm (BCRF) is the researchers’ equipment needs. The Research Equipment Assis- first-in-the-nation integrated research and demonstration facil- tance Program (REAP) maximizes the use of research and teaching ity dedicated to biomass production and processing. It is located equipment by maintaining a locational database and loan program seven miles west of the Iowa State University campus on U.S. through which interdepartmental loaning and sharing of equip- Highway 30. The BCRF offers opportunities for large pilot scale ment is conducted. The REAP office has in inventory various and pre-commercial scale research in biomass feedstock produc- pieces of research equipment that are available for loan. For more tion, harvest, transport, storage, preparation, processing and information, call 515-294-5752. laboratory testing. Field plots for crop production trials, field equipment modification and testing facilities, biomass storage fa- For General Information cilities and biomass conversion facilities are available for internal Please see our website at www.biotech.iastate.edu or contact us by and external users. email at [email protected].

61 Research in Biotechnology 2020 The biomass processing facility has three 2,700-square-foot pilot Magnetic Resonance plant processing trains which house biochemical and thermo- Six NMR spectrometers of varying frequencies from 400 to 600 chemical processing systems, four laboratories totaling 2,100 MHz are available. Chemical structure, atomic spatial orientation, square feet, and over 1,400 square feet of office space. The BCRF and molecular diffusion are determined by using this technology. also has 4,200 square feet of dry biomass feedstock storage and A fully-equipped electron paramagnetic resonance spectrometer, 420 square feet of cold storage. used in the detection and characterization of free radicals and paramagnetic ions, is also available. Equipment located in the Services available at the BCRF include biomass feedstock produc- facility includes a Bruker Avance II 600 NMR with cryoprobe- tion; biomass preparation (sizing, drying and chemical pretreat); solution and solids capability; Bruker Avance III 600 and DRX- fermentation production of fuels, industrial chemicals, and 500 and Agilent MR-400 (solution) NMRs; an Avance 400 for other products (500- and 1,000-liter capacities); thermochemi- dedicated solid-state NMR; and a Bruker NEO-400 equipped with cal processing via fast pyrolysis at a rate of 22 kg/hour; solvent flow cell, reaction kinetics, and LC-NMR capabilities. There is liquefaction at a rate of 1 kg/hour; and gasification at a rate of 35 also a fully-equipped Bruker E-580 FT-EPR. kg/hour. The BCRF also has a 720-square-foot algal production facility capable of producing algal biomass of 1.3-4.5 kg/week and Mass Spectrometry a biopolymer processing plant which can process 500 kg/day. The mass spectrometry lab is equipped to provide low- and high-resolution GC-MS on mixtures, pyrolysis GC-MS, and high- More information is available online at www.biocenturyresearch- resolution measurements for determining the elemental composi- farm.iastate.edu. tion of pure samples and multi-component mixtures. GC x GC capability is now offered. Electrospray and APCI are used rou- tinely for the ionization of medium and higher molecular weight Biomolecular Nuclear Magnetic compounds, including synthetic organics and compounds of biological origin. Two dedicated LC-MS instruments are available Resonance Facility for routine work and special projects. MS-MS (parent-daughter relationship) experiments are routine. Equipment located in the Location: 0208 Molecular Biology Building facility includes a Waters Micromass GCT-GCMS equipped with Hours: 9 a.m. to 5 p.m. weekdays or by appointment a pyrolysis unit, an Agilent 5973 GCMS, an Agilent 7250 GC- Contact: Bruce Fulton, Manager, [email protected], QTOF, a Shimadzu LCMS2020, and an Agilent 6540 Q-TOF LC 515-294-2139 MSMS. The Biomolecular Nuclear Magnetic Resonance (NMR) Facility is supported by the Roy J. Carver Department of Biochemistry, Preparative HPLC Biophysics and Molecular Biology. The facility currently operates The Agilent PrepStar SD-1 preparative HPLC system is capable of Bruker Avance II 700 and Avance III 800 spectrometers, each flows ranging from 1-2 ml/minute (analytical) to 200 ml/minute capable of performing a broad range of modern multi-nuclear, (preparative). The system includes two pumps, a dual-wavelength multi-dimensional NMR experiments on biomolecules. The 700 detector, fraction collector and complete data system for instru- is equipped with a 5 mm H/C/N cryoprobe, a 5 mm H/C/BB con- ment control and data processing. Both semi-prep (9 mm i.d.) ventional probe and a 3.2 mm H/BBX/BBY-MAS solids probe. The and preparative (21 mm i.d.) columns are available for use for an 800 is equipped with a 5 mm H/C/N cryoprobe, a 5 mm H/C/ additional fee. BB conventional probe and 3.2 mm H/C/N-P and H/C/D solids probes. X-ray Diffraction A completely-equipped X-ray diffraction laboratory provides The facility has computational resources for processing and instrumentation for the study of the molecular structures of small analyzing NMR data and obtaining molecular structures. The molecules and powders. The equipment includes Bruker APEX II facility provides consultation on the application of NMR to solve CCD and VENTURE D8 single-crystal diffractometers equipped research problems. NMR data can be acquired and interpreted as with low-temperature devices, and a Rigaku Ultima 4-powder dif- an analytical service. Projects larger in scope can be pursued on fractometer available for general use. The facility’s crystallographer a collaborative basis. In the latter case, the facility will provide provides complete reports that are suitable for publication. training and guidance for researchers to operate instruments and interpret data. Spectrophotometry A variety of spectrophotometers is available for routine use in the facility. These instruments provide fingerprint spectra for characterizing and identifying compounds. These instruments currently Chemical Instrumentation Facility include a Horiba time-correlated single photon counting fluorom- Location: 1234 Hach Hall eter, Bruker Tensor 37 FT-IR, a Hewlett-Packard HP-8453 Diode Hours: 24 hours a day, seven days a week; professional Array UV-Vis, a Cary 100 Bio double beam UV-Vis, a Jasco J-710 help available from 8 a.m. to 5 p.m. weekdays circular dichroism spectrophotometer, and two polarimeters. Contact: Sarah Cady, Manager, [email protected], 515-294-5805 Isothermal Titration Calorimetry (ITC) Web: www.cif.iastate.edu A Microcal VP-ITC is available to study the thermodynamics of chemical reactions initiated by the addition of a binding compo- The Chemical Instrumentation Facility has more than five million nent. It is often used to characterize biomolecular interactions. dollars’ worth of analytical instrumentation available to faculty, graduate students, industry and other educational institutions. Elemental Analysis The staff of five highly-qualified professionals supports university A Thermo FlashSmart CHN/S elemental analyzer is available for research by keeping the analytical equipment available and oper- sample submission or for investigator use. Normally, the instru- able and by providing application support and user training. Ser- ment is configured for carbon, hydrogen and nitrogen, but sulfur vices available to the research community include the following: also can be analyzed upon special request.

62 Services and Facilities Comparative Pathology Core Service CCUR administers and works closely with the Iowa State University Fermentation Facility. Location: 2712 Veterinary Medicine More information is available online at www.ccur.iastate.edu/. Hours: By appointment, Monday through Friday, 8 a.m. to 5 p.m. Contact: Rachel Phillips, [email protected], DNA Facility 515-294-0953 Web: vetmed.iastate.edu/vpath/services/comparative- Location: 1184 Molecular Biology Building pathology-core-service Hours: By appointment, 7:30 a.m. to 5 p.m. weekdays Contact: Michael Baker, Manager, [email protected], The Comparative Pathology Core Service (CPC) provides pathol- 515-294-4705 ogy support services and consultation for investigators working Web: dna.iastate.edu with animal models of human and animal diseases. The veterinary pathologists in the CPC provide gross and microscopic The DNA Facility of the Office of Biotechnology performs Sanger tissue examination and clinical pathology evaluation. DNA sequencing, next-gen (Illumina and Oxford Nanopore) sequencing, plant genomic and plasmid DNA extraction, DNA In addition, the core can help design, develop and implement and RNA characterization, automated fluorescent genotyping, ancillary techniques including immunohistochemistry, RNAscope DNA synthesis and primer walking, and quantitative and digital (in situ hybridization), fluorescence microscopy, morphometry, PCR. Clients may submit orders through the OnCore LIMS special stains and slide imaging. The CPC also offers in-depth system. This software allows clients to track the progress of their analysis of these and other techniques using HALO image analy- orders and automatically notifies them when their data are ready sis software. Costs are based on histopathology lab services and to download. assistant scientists’/pathologists’ time. More detailed information can be obtained by contacting Rachel Phillips. Sanger Sequencing (www.dna.iastate.edu/sangersequencing.html) More information is available online at vetmed.iastate.edu/vpath/ Sequencing samples are run on the DNA Facility’s Applied services/comparative-pathology-core-service. Biosystems 3730xl DNA Analyzer. The AB 3730xl provides up to 900 bases of usable sequence data per reaction and is capable of processing 12 sets of samples in a 24-hour period. DNA can Center for Crops Utilization Research be sequenced as plasmid, lambda, cosmid or BAC DNA, or as PCR products. Custom primers can be used with all types of Location: 1041 Food Sciences Building (Administration) templates. When no problems are encountered with a template, 1955 Food Sciences Building the results are generally returned within 48 hours after receipt of (Pilot Plant Office) the samples. A four-color printout of the data can be provided, if Hours: 8 a.m. to 5 p.m. weekdays (fall, winter, spring) needed. The facility also provides a primer walking service (www. 7:30 a.m. to 4 p.m. weekdays (summer) dna.iastate.edu/primerwalking.html). Contact: Zhiyou Wen, Interim Director, wenz@iastate. edu, 515-294-0426 For clients with high-throughput sequencing projects, samples Web: ccur.iastate.edu/ can be submitted in a 96-well format. The facility should be contacted prior to submission of samples in 96-well format. The Center for Crops Utilization Research (CCUR) is a resource to assist Iowa State researchers and external businesses with NextGen Sequencing developing new processes, products and markets for corn, (www.dna.iastate.edu/nextgensequencing.html) soybean and other crops. CCUR strives to add value to grain and The Iowa State DNA Facility operates both an Illumina plant materials by conducting grant and contract research; offer- HiSeq3000 and an Illumina MiSeq. These instruments can ing short courses, workshops, seminars and training; performing produce up to 400 million (HiSeq 3000) and 25 million (MiSeq) analytical testing, pilot plant processing and consumer evaluation sequencing reads per lane, respectively, making them ideally- services; providing technical consulting services; and offering suited for many applications, including ChIP-seq, genome small-business incubator services. The center has state-of-the-art resequencing, mRNA and small RNA sequencing, and 16S laboratory and pilot plant processing and analytical equipment. amplicon sequencing. The facility provides library preparation services for these applications as well as many of the Chromium The pilot plant facilities include a 5,000 square-foot wet-pro- applications, including single cell RNA, linked reads, single cell cessing pilot plant (e.g., soy protein isolation, corn wet milling); ATAC-seq, and immunoprofiling. Clients can choose various read a 2,600-square-foot dry-processing pilot plant (e.g., dry corn lengths and either single or paired-end sequencing. Depending on milling, drying, grinding, sieving); a 3,000-square-foot product the application, up to 384 samples can be multiplexed per lane. processing and technology transfer pilot plant (e.g., food and feed processing; plastic extrusion, molding, and film blowing; packag- The facility also operates an Oxford Nanopore GridION ing; building materials manufacturing); and various process sequencer. This instrument is capable of providing very long development and analysis laboratories (e.g., chromatography, reads. Depending on the quality of the genomic DNA, average grain analysis, vegetable oil refining, baking, process develop- read lengths of 13,000 base pairs or more can be achieved, with ment). some reads greater than 150,000 base pairs per run, producing a total of 10 to 15Gb of data per flow cell. It is well-suited The sensory facility has the space and capabilities for both trained for de novo assemblies, for full-length transcript sequencing, and consumer panels. Consumer evaluation includes preference or in conjunction with existing short-read data to fill gaps in and acceptability tests in private booths. Trained panel evalua- assemblies. The facility provides library preparation services for tion, such as descriptive analysis, takes place in one of two focus most Oxford Nanopore applications. group rooms and private booths. A small theater and conference facilities are available for technology transfer activities.

63 Research in Biotechnology 2020 Genotyping from initial project design through chemistry and material (www.dna.iastate.edu/genotyping.html) selection and data analysis. The facility can process a number of marker types including SSRs, AFLPs, SNPs, ISSRs, and T-RFLPS using an Applied Biosystems More information is available online at www.dna.iastate.edu. 3730 DNA analyzer to electrophorese samples and collect the image data. Each sample can have as many markers as the client can identify. The data are analyzed using Applied Biosystems’ Doubled Haploid Facility GeneMapper Analysis software, and electronic files are provided Location: 1204 Agronomy Hall via the facility server. Hours: 8 a.m. to 5 p.m. weekdays Contact: Thomas Lübberstedt Oligonucleotide Synthesis [email protected], (www.dna.iastate.edu/oligosynthesis.html) 515-294-5356 The DNA synthesis service synthesizes DNA oligomers in two scales – 50-nmol and 200-nmol – and can make modified The Doubled Haploid Facility (DHF) provides haploid doubling oligomers such as the fluorescent primers used in genotyping for maize to Iowa State scientists, as well as external academic applications. Oligos are synthesized using a BioAutomation researchers and breeders. The facility uses laboratories, green- MerMade-192 DNA synthesizer. In addition, primer design for houses and nurseries provided by the Department of Agronomy. primer walking sequencing projects is available at www.dna. The development of homozygous lines is an important, but time- iastate.edu/primerwalking.html. consuming, process in plant breeding and research. The induction and subsequent doubling of haploids is an efficient alterna- DNA Template Preparation and Miscellaneous Services tive to generate homozygous offspring in two generations.

Plasmid Preparation Service The DHF also develops inducer lines with higher adaptation (www.dna.iastate.edu/plasmiddnaextraction.html) to the growing conditions of the Midwest, additional selectable The facility offers two different 96-well format plasmid preps – markers for haploid detection and for use in specialty crops such Millipore’s Montage prep and a lower cost screening prep. as popcorn.

Plant Genomic DNA Extraction Service The mission of the DHF is to provide expertise and service in (www.dna.iastate.edu/plantdnaextraction.html) the production of doubled haploid lines in maize, improve the The facility performs plant genomic DNA extraction using technology in order to get higher success rates and lower costs the AutoGen 740 Automatic DNA Isolation System, a fully- and to teach and train scientists and students. automated instrument that is capable of extracting 192 samples per day. Extraction can be performed on either fresh or preferably The three main steps that comprise the doubling of haploid lines lyophilized material from a variety of plant tissues. The facility are haploid induction by pollination with inducer lines, selection is capable of extracting high quality genomic DNA from plant for haploid kernels, and doubling of haploid plants and subse- tissues suitable for sequencing on the GridION. quent seed production

Plant Tissue and Seed Grinding Service Haploid Induction (www.dna.iastate.edu/tissuegrinding.html) For haploid induction, maternal inducer genotypes developed Plant tissues and seeds are ground in polycarbonate tubes using on the basis of RWS described by Roeber et al., 2005, are used. stainless steel ball bearings. Entire, intact seeds can be ground The average induction rate is 8-12%. into fine powder. Haploid Kernel Selection DNA and RNA Characterization Kernels with a haploid or F1 embryo can be distinguished by (www.dna.iastate.edu/bioanalyzer.html) means of the expression of the dominant anthocyanin marker The facility has two instruments available to assist with nucleic gene R1-nj which provides a red pigmentation of the embryo acid sizing and quantification. The Agilent Bioanalyzer is best- and the endosperm. Kernels with a pigmented endosperm and a suited to smaller numbers of samples with sizes up to 12kb in non-pigmented embryo are selected as haploids. In materials dif- length; the Advanced Analytical Fragment Analyzer can quickly ficult to select based on R1-nj, additional selection for haploids and economically process larger numbers of samples up to can occur at the seedling stage using the red root marker (Pl1). 200Gbp in length. The facility also has a Qubit instrument for DNA/RNA quantitation, a Covaris ME220 for DNA shearing, Doubling a Sage Blue Pippin for DNA size fractionation and a plate Spontaneous chromosome doubling occurs but is dependent on reader capable of running a number of different assays. These the genotype. To obtain more consistent doubling rates, plants instruments are available for client use (training available) or as are treated with colchicine following the procedure. Doubling facility run. rates of up to 25% are realized. The facility calculates with an average of about 10% to determine sizes of experiments for Quantitative and Digital PCR customers.The production of the double haploid lines requires (www.dna.iastate.edu/quantitativepcr.html) two generations. For one generation, a winter nursery in Chile is The DNA Facility maintains a quantitative, real-time PCR used. instrument, the Applied Biosystems StepOnePlus, and will accept jobs on a ready-to-run basis. Applications include gene expression Spontaneous haploid genome doubling (SHGD) studies, validation of microarray data, allelic discrimination, A germplasm with high spontaneous haploid genome doubling SNP analysis and screening for GMOs. For clients with projects is now available. The trait is conferred by one major QTL. Donor that require a higher throughput, the facility has available the lines for SHGD are available for licensing. Contact the facility for Fluidigm® BioMark™ HD system. This instrument utilizes more information. microfluidic technology to accurately combine reagents and samples to perform thousands of individual nanoliter-scale PCR More information is available online at www.plantbreeding.iastate. or genotyping assays in a single run. DNA staff are also available edu/DHF/DHF.htm. to advise or assist clients at any point in the experimental process 64 Services and Facilities Fermentation Facility lipophilic dyes are available that have been utilized as a means of tracking cell life and tissue localization in vivo. Levels of enzyme Location: 1621 Food Science Building (campus location) marker gene expression, such as beta-galactosidase, can be cor- 1327 U Avenue, Boone, IA related with the fluorescence intensity of cleaved substrate by (BioCentury Research Farm) flow cytometry. Intracellular protein products can be measured Hours: 8 a.m. to 5 p.m. weekdays by immunofluorescent labeling of fixed cells. Fluorescencein Contact: Zhiyou Wen, Interim Director, situ hybridization (FISH) techniques provide information on the Center for Crops Utilization Research mRNA expression level of a specific gene and can be used in 515-294-0426 conjunction with flow cytometry to provide quantitative gene Web: ccur.iastate.edu/fermentationfacility/ expression information on a cell-by-cell basis. Necrotic versus apoptotic-mediated cell death can be distinguished using flow The Fermentation Facility is designed to help researchers develop cytometry. It is also possible to sort individual cell populations new fermentation technologies and products and provide bench- via flow cytometry, enabling researchers to separate and further top- and pilot-scale fermentation equipment. The facility has characterize subpopulations of cells. equipment for scaling up the production of industrially-important chemicals, chemical feedstocks, genetically-modified organisms Flow Cytometry Data Acquisition and Cell Sorting and enzymes. The Flow Cytometry Facility maintains two flow cytometers for data acquisition and cell sorting: a BD Biosciences FACSAria III Equipment housed in the Food Sciences Building includes four and BD Biosciences FACSCanto. The facility also maintains sev- benchtop fermentors with 5-liter and 7-liter working volume eral computer workstations with software packages for perform- capacities and pilot-scale fermenters with 15- to 120-liter working ing off-line analysis of flow cytometry data. volume capacities. Downstream processing equipment includes a hollow fiber ultrafiltration system, semi-continuous centrifuge, BD Biosciences FACSAria III French pressure cell press, bio-hood and an eight-shelf freeze The BD FACSAria III is a sorting flow cytometer. The power dryer with stoppering capability. of multi-color flow cytometry and the highly sensitive analysis platform of this instrument allow investigators to categorize an The facility also manages large-scale fermentation activities con- almost endless variety of unique cell subpopulations. Once cells ducted at the BioCentury Research Farm (BCRF) that are focused of interest are identified, the high-speed sorting platform of the on industrial chemicals, biobased products, and biofuels. Pilot- FACSAria III can simultaneously isolate and collect these target scale equipment housed in the Biomass Processing Facility at the events for further study. BCRF, located seven miles west of Ames on U.S. Highway 30, includes two Applikon fermentors with working volumes of 500 The FACSAria III has three excitation lasers (405, 488 and 633 and 1,000 liters, distillation tower, falling film evaporator, rotary nm) and is configured to detect nine fluorescent parametes steam tube dryer, Sharples P660 continuous horizontal decanting (Pacific Blue, AmCyan, FITC, PE, PE-TexasRed, PerCP-Cy5.5, centrifuge, stirred tanks, pumps, and scales. PE-Cy7, APC and APC-Cy7), as well as forward and side scatter. This gives investigators extensive flexibility in reagent selection More information is available online at www.ccur.iastate.edu/fer- and experimental design. The FACSAria III has the capacity for mentationfacility. 11-parameter detection on particle sizes ranging from 0.5 to 50 μm in diameter. Thus, viable cell, aseptic sorts can be performed on a wide range of cell types (i.e. bacteria, mammalian leukocytes, Flow Cytometry Facility plant protoplasts, etc.). Up to four unique populations can be sorted simultaneously into 1.5 mL microtubes, 12 x 75 mm, or Location: 1104 (Office) or 1117 (Lab) Molecular Biology 15 mL tubes. Cells can also be sorted into several different types Building of tissue culture plates and slides, and both sample and collection 1120 Veterinary Medicine Complex chambers can be maintained at pre-determined temperatures. (Satellite facility) Hours: 8 a.m. to 5 p.m. weekdays, by appointment Digital electronics allow the FACSAria III to achieve data acquisi- Contact: Shawn Rigby, Manager, [email protected], tion rates as high as 70,000 events per second. For many com- 515-294-2472 mon sorting experiments, an event rate of 25,000 per second will Web: flow.biotech.iastate.edu consistently attain ≥98% sort purity and ≥80% of expected yield. Higher event rate sorts (i.e. 50,000 – 70,000 per sec.) can be The Flow Cytometry Facility of the Office of Biotechnology achieved in many instances with minimal loss of sort purity and offers flow cytometric analysis and cell sorting for a wide range only moderate loss of expected yield. of research applications. Facility personnel are trained to assist researchers in flow cytometry experimental design, sample prepa- The FACSAria III is located in the Molecular Biology Building ration techniques, and data analysis. Training sessions to provide main facility and is operated by facility personnel only. Data ac- general instruction in these areas are also offered in the facility. All quired on the FACSAria III is loaded onto a network server which facility services are open to internal Iowa State University clients, allows the remote retrieval of data files via an Ethernet connec- as well as to external institutions and individuals. tion. The Flow Cytometry Facility also maintains a backup of user data. An appointment to schedule an experiment on the FACSAria Flow cytometry is used to analyze bacteria, mammalian cells, III can be made by simply calling or emailing Flow Cytometry fungi, yeast, insect cells, microscopic parasites, plant cells, nuclei, Facility personnel. organelles and chromosomes. Immunofluorescence measurements are used to provide information on intracellular and cell surface BD Biosciences FACSCanto receptor densities. DNA/RNA-specific stains supply information The FACSCanto is a data acquisition flow cytometer that com- on genome size, chromatin structure, and cell cycle kinetics. bines a patented optical design, digital electronics and a novel Fluorochromes are available for quantitating a number of cellular sample injection system supporting carryover of less than 0.1%. metabolic functions, such as calcium mobilization and oxidative High-speed data processing, industry-leading sensitivity and burst. Phagocytosis of fluorescently labeled particles (beads, yeast minimal sample-to-sample carryover make this instrument or bacteria) can be quantified. Cell viability and concentration can uniquely suited for rare event analysis. The FACSCanto has the be measured for mammalian cells, as well as bacteria. Fluorescent capacity for 16-parameter detection on particle sizes ranging from 65 Research in Biotechnology 2020 0.5 to 50 μm in diameter, which includes leukocytes, cell lines, knowledge into pipelines that are easy to understand and use by platelets, bacteria, multiplexed bead technologies and more. faculty, staff and students and to facilitate data analysis that leads to the transformation of Big Data into data that dramatically accel- The FACSCanto has three excitation lasers (405, 488, and 633 erates our understanding of biology and evolutionary processes. nm) and is configured to detect 14 fluorescent parameters (including, but not limited to, FITC, PE, PE-TexasRed, PerCP-Cy5.5, Available Services PE-Cy7, APC, Alexa Fluor 700, APC-Cy7, BV421, BV510, BV605, BV650, BV711, and BV785), as well as forward and side scatter. • Grant review (methods, data management plans, outreach) Data are acquired with digital electronics. The system can handle • Figure generation for papers high sample flow rates (up to 120 ml/min.) and fast acquisition • Experimental design review rates (up 10,000 events/sec.). Digital electronics also facilitate • Genome assembly and annotation compensation, with no limits to inter- and intra-beam compensa- • Transcriptome assembly and annotation tion, allowing post-acquisition compensation. Data files are stored • SNP/InDel calling raw and compensated as part of the FCS files, allowing flexibility • RNA-seq analysis for off-line compensation when viewing data. • Single cell RNA-Seq analysis • ChIP-seq The FACSCanto instrument is located in the Molecular Biology • Proteomics analysis Building main facility and is operated by facility personnel only. • Metagenomics analysis FACSCanto-generated data are uploaded to cloud storage which • Training of students, postdocs and staff allows customers to retrieve data files from their office or lab via internet web browser. The Flow Cytometry Facility also maintains To request bioinformatics support or service, please email the a backup of user data. An appointment to schedule an experiment facility at [email protected]. Include in the email the type of on the FACSCanto can be made by contacting facility personnel. organism and the types of analyses envisioned. An appointment can then be made to further discuss the project. Large-Scale Cell Separation The facility maintains an Miltenyi Biotec AutoMACS Pro More information is available online at gif.biotech.iastate.edu/. magnetic cell separation instrument within the Veterinary Medicine Complex satellite office. The AutoMACS Pro is a fully- Genomic Technologies Facility automated bench-top sorter that can be used to perform sterile bulk sorts. Designed for ultra high-speed positive selection, as Location: 2025 Roy J. Carver Co-Laboratory well as depletion, the AutoMACS Pro can isolate virtually any cell Hours: By appointment type and is compatible with almost any direct or indirect MACS Contact: Lakshmi Attigala, Overall Management reagent. Users need only label cells to be sorted and choose [email protected], 515-294-8563 an AutoMACS Pro separation program. The separation is done Web: www.plantgenomics.iastate.edu automatically. Several separation programs can be selected from a touch screen menu. By using the positive selection program, the The Genomic Technologies Facility (GTF), an Iowa State core lab- AutoMACS Pro is capable of isolating up to 2 ×108 pure target oratory in the Plant Sciences Institute, provides access to equip- cells within minutes. Cells as rare as 1 in 106 can be enriched to ment for genomic research. In-house next generation sequencing high purity through the use of double positive selection programs. can be performed on the Ion Proton™ platform. One real-time “Untouched” cells may be obtained just as easily by depleting thermocycler, a Roche LightCycler 480, is available to conduct unwanted cells with the AutoMACS Pro depletion programs. The high-throughput gene expression analysis. The GTF offers Agilent AutoMACS Pro and associated reagents are completely compatible Bioanalyzer, Sage Science Blue Pippin, Eppendorf AF2200 Plate with flow cytometry. Fluorescent and magnetic labeling of cells Reader, Life Technologies Qubit and Qiagen BioSprint instru- can be performed simultaneously. After AutoMACS Pro sorting, ments for DNA/RNA quantification, fragment size selection and cells are immediately ready for flow cytometric analysis. The DNA/RNA extraction. AutoMACS Pro is user-operated. Potential users must complete a mandatory training session, administrated by facility personnel, Life Technologies™ Sequencing Services (Ion Proton™) which is provided upon request. Ion Proton™ Sequencing and Custom Library Preparation can be completed in two days. Template library sequences are amplified Additional Laboratory Equipment on Ion Sphere Particles and checked for quality. An enrichment A refrigerated table-top centrifuge, carbon dioxide incubators, step purifies the amplified template, and sequencing is performed biohazard hood, temperature-controlled water baths, analyti- using Ion Proton’s semiconductor ion chips. During this process, cal balances, pH meter, refrigerators, freezers and a cryostorage nucleotides are sequentially incorporated into DNA, causing a hy- system are available. drogen ion to be released. With the Ion Proton™ P1 chip, 200 bp single end sequencing can provide up to 80-100 million reads per More information is available online at flow.biotech.iastate.edu. run. Sequencing runs can be conducted by on-campus customers on a fee-for-service basis. Genome Informatics Facility Ion Proton™ preparations available include: Ion Xpress Plus Location: 451 Bessey Hall Fragment Library and Ion Total RNA-Sequencing. Hours: 8 a.m. to 5 p.m., weekdays Contact: Andrew Severin, Manager After preparation, libraries are quantified to ensure proper load- [email protected], 515-294-1320 ing. Users can quantify libraries using the GTF’s Bioanalyzer. Web: gif.biotech.iastate.edu/ Before accessing facility equipment and services, users are re- The Genome Informatics Facility (GIF) of the Iowa State Univer- quired to provide a signed and approved user agreement to facil- sity Office of Biotechnology provides bioinformatics services for ity staff. After training, users can operate equipment and conduct investigators within academia, industry and the government. It analyses themselves. Reservations to use instrumentation may be serves as a centralized resource of expertise on the application made using the facility’s on-line scheduler. More information can of emerging sequencing technologies and open source software be found online at www.plantgenomics.iastate.edu. as applied to biological systems. Its mission is to integrate this 66 Services and Facilities Grain Quality Laboratory Roy J. Carver High Resolution Microscopy Facility Location: 3167 National Swine Research and Information Center Location: 0122 Molecular Biology Building Hours: 8 a.m. to 5 p.m. weekdays, other times by (Atomic Force, Electron, Hyperspectral and appointment Light Microscopy) Contact: Glen Rippke, Manager, [email protected], 515-294-5387 0116 Molecular Biology Building Web: www.iowagrain.org (Confocal and Multiphoton Microscopy, Laser Capture Microdissection [LCM] The laboratory provides instrumental analyses of chemical and and Image Analysis) physical properties of grain and other agricultural products. The Hours: 8 a.m. to 5 p.m. weekdays, by appointment services are intended to support high-throughput genetic evalu- Contact: Email: [email protected] ations, surveys of grain quality and other large-scale monitoring Curtis Mosher, Manager, Atomic Force, operations. The facility also calibrates various types of instru- 3D and Hyperspectral Microscopy ments for manufacturers and users. 515-294-6513 Chemical composition is measured with a non-destructive, near- Margie Carter, Manager, Confocal and infrared (NIRS) analyzer. For best accuracy, this test requires 400 Multiphoton Microscopy, LCM, and Image grams or more of product. Sample sizes down to 150 grams will Analysis, 515-294-1011 work with corresponding reductions in accuracy. Tracey P. Stewart, Manager, Crop-specific tests Electron and Light Microscopy 515-294-3872 • Corn: moisture, protein, oil, starch and density; Ethanol yield and feed value can be calculated from the NIR results • Soybeans: moisture, protein, oil, fiber, total saturates and The Roy J. Carver High Resolution Microscopy Facility (HRMF) of linolenic acid; Meal and oil processed value (EPV) can be the Office of Biotechnology provides a variety of instrumentation, calculated from the NIR results technical assistance, consultation and training to individuals and • Soybean meal: moisture, protein, oil and fiber groups of life sciences and biotechnology researchers who want • Distillers grain: moisture, protein, oil and fiber to image biological samples by using atomic force microscopy, photomacrography, laser capture microdissection (LCM), light General Tests microscopy, scanning electron microscopy, transmission electron microscopy, confocal and multiphoton microscopy, optical and • Seed weight and size hyperspectral workstations, cryopreservation, cytochemistry, to- • Test weight mography, x-ray microanalysis and image analysis. In addition to • Thins (corn) the round-the-clock availability for trained individuals, the HRMF • Specific gravity (measure of hardness) by nitrogen displace- carries out service work for both on- and off-campus researchers. ment The staff of the HRMF is available for consultation and individual • Other contracted services help. For imaging material samples, please contact the • Adaptation of NIRS instrumentation for individual situa- Materials Analysis and Research Laboratory. tions and specialty measurements is provided upon request. • The facility’s calibration service program creates calibrations Atomic Force and 3D Microscopy for electronic measurement equipment of various types and trains users in quality control validation. These can be done Digital Instruments Dimension™ 3000 for instrument manufacturers or for individual users on a wide range of products and instrument types. Scanning Probe Microscope The Dimension 3000 scanning probe microscope (SPM) brings The lab has an in-house quality management system to verify together all SPM techniques in a single platform and handles accuracy and reproducibility of data. Advice and set-up of quality a wide range of sample sizes and types. A rigid, low vibration control programs for analytical data can be provided. construction of the Dimension 3000 SPM ensures the highest quality images and measurements. More information is available online at www.iowagrain.org. Samples up to eight inches in diameter can be scanned in ambient air or fluids using the Dimension 3000 SPM. The Dimen- sion 3000 SPM requires little or no sample preparation, and the simple vacuum mounting system allows easy and convenient setup. Superior linearity and resolution in all three dimensions are obtained, even for large samples. Integrated top-view video optics with motorized zoom and 1.5 µm optical resolution help identify areas of interest for detailed scanning quickly and easily. Changing scanning techniques, for example from AFM to STM, requires no tools.

Digital Instruments MultiMode with Tapping Mode™ The MultiMode system features multiple scanners that permit the user to tailor the system for individual research. Scanners with large scan ranges up to 120 microns on the X–Y axes and a Z range up to 6 microns, as well as high-resolution scanners

67 Research in Biotechnology 2020 with 0.5 microns X–Y axes and submicron Z range, are avail- ton laser to include the red and high red ranges, enabling use of able. The vertical-engage “JV” and “EV” scanners allow the tip essential fluorophores such as mCherry, DsRed2, mRFP, Texas to be positioned at any point on the surface, without adjust- Red, Alexa Fluor 660 and others. Researchers are able to precisely ing for lateral movement during approach. The MultiMode is set the emission wavelengths they would like to capture. controlled with a NanoScope IIIa controller. This controller provides 16-bit resolution on all three axes, with three indepen- A Zeiss 780 confocal microscope system is available. This system dent 16-bit digital-to-analog converters (DACs) in X and Y for has five lasers allowing excitation wavelengths of 405, 458, 488, control of the scan pattern, scaling and offset. This configura- 514, 561, 594, and 633 nm and is mounted on an AxioOb- tion provides 16-bit resolution of the lateral scanning motion at server Z1 inverted microscope. It is equipped with two PMTs, any scan size. one 32-channel GaAsP detector and one transmission detector. Emission wavelengths between 290 and 750 nm can be detected. Digital Instruments Dimension™ 3100 Scanning Probe A variety of dry, water, multi-immersion and oil objectives rang- Microscope ing in magnification from 2.5x to 100x are available. Images are The Dimension 3100 is controlled with a Nanoscope IV con- acquired and analyzed using Zeiss Zen Black software. troller. The NanoScope IV features up to ten-times-faster scanning, as well as increased functionality, bandwidth, flexibility Electron Microscopy and expandability. Electron microscopy instrumentation includes a 200kV JEOL 2100 scanning/transmission electron microscope (STEM) with Sensofar S neox Non-contact 3D Surface Profiler elemental analysis, cryo-imaging, tomography, and image The S neox uses Sensofar’s patented technology, based on a analysis systems, and a Hitachi SU4800 FE-SEM field emission microdisplay. The ferroelectric liquid crystal on silicon (FLCoS) scanning electron microscope. microdisplay is a fast switching device that makes the scanning of confocal images fast and very stable with additional interfer- The JEOL 2100 STEM, with <1.4Å resolution, consists of the ometry and focus variation scanning options. Combining this basic STEM, a Thermo Fisher Scientific Pinnacle System light- approach with Sensofar’s high NA objectives (0.95), vertical element energy dispersive x-ray spectrometer (EDS) and resolution is reduced to below 1 nm with state-of-the-art lateral integrated software package for computerized control. The digital resolution. microscope and analytical system allow elemental analysis of the composition and structure of specimens with a nanometer resolution. Special features include a darkfield/brightfield detector Confocal and Multiphoton Instrumentation (STEM), a backscattered electron detector (BSE), high-angle tilt Confocal microscopy allows for real-time optical sectioning of holders for tomography, cryo-preparation system and holder for fixed and living specimens, providing significant improvements in cryo-TEM imaging, and a Gatan One View 4K digital camera for optical contrast and resolution over traditional light and fluores- image recording. cence microscopy. The facility is equipped with a Leica SP5 X MP confocal/multiphoton microscope system with an inverted micro- Ancillary equipment available for preparation of specimens for scope front end. Technology available on this system includes a electron microscopy include a Denton critical point apparatus, white light laser, IR laser and optical parametric oscillator (OPO). Cressington 208HR sputter coater, HPM-100 cryo-fixation system, LEICA AFS II freeze substitution system, two LEICA UC6 Researchers are no longer limited to three or four lasers at three ultramicrotomes, a LEICA UC6 with cryo-sectioning system, and or four fixed wavelengths but can tune the white light laser to any a Leica glass knife maker. wavelength between 470 and 670 nm, which has a similar effect to having 200 lasers and 200 wavelengths. This allows research- The field emission scanning electron microscope (Hitachi SU4800 ers to select the excitation wavelength that is best suited to their FE-SEM) offers excellent resolution (1.3nm at 1kV) and can samples and will result in maximum fluorescence emission. On operate at ultra low voltages (100-500 volts) to provide high- the emission side, the system has an Acousto-Optical Beam Split- resolution imaging of sensitive samples. This system has a ter (AOBS) which allows researchers to precisely set the emission Quorum cryo-imaging system and a Thermo Fisher Scientific wavelengths they would like to capture rather than be limited Pathfinder elemental imaging/analysis (x-ray) system. to preset emission ranges determined by fixed filter sets. Both of these technologies help to separate fluorescence signals with close excitation and/or emission spectra. Light Microscopy Light microscopy instrumentation includes Zeiss AxioImager, Other features include faster scanning, higher resolution and in- AxioObserver, and Macrozoom imaging systems that are fully creased sensitivity. Researchers will now be able to work with live automated, digital, and offer multiple fluorescent filters for cells over an extended period using live cell equipment, including multi-mode imaging, including tiling, z-stacking and time lapse. a heated stage with microcontainment or macrocontainment sys- The HRMF also has a Zeiss Axioplan II compound microscope tem, active gas regulation, cell cultivation chamber and microin- equipped with AxioCam color and B/W digital cameras and the jection system. For capturing images of fast-moving samples or following optical modes – bright-field, phase-contrast, polarizing, events that occur in a fraction of a second, the resonant scanner dark-field, fluorescence and Nomarski (DIC). In addition, this enables video rate scanning with speeds up to 16,000 lines per microscope has an Apotome for creating thin optical slices; an second. The system also has FRET, FRAP, timelapse, hyperspectral Olympus stereomicroscope with reflected and transmitted light signal separation software, 3D imaging, colocalization, decon- sources and a high-resolution digital color camera; dissecting volution, region of interest (ROI) scanning, brightfield and DIC microscopes; and Olympus compound microscopes, one with capabilities. In addition to the white light laser, the system has a a digital camera. Microscopes with digital cameras have image 405 laser providing UV excitation and an Argon laser providing analysis capabilities. CFP excitation and extra power for bleaching. Preparative equipment and space for light microscopy include The system allows users to easily switch between confocal and fixing and processing hoods; paraffin and resin embedding areas; multiphoton mode. In multiphoton mode, users can image deep- rotary-, cryo- and ultra-microtomes; cryostat, knife maker; knife er into their samples than is possible with confocal systems. The sharpener, Vibratome, vacuum oven, and centrifuges. higher excitation wavelengths are less damaging to live samples, as well. The OPO expands the excitation range of the multipho-

68 Services and Facilities Hyperspectral, Optical and Fluorescence Workstation projects are usually screened and selected by the client; how- The workstation consists of an inverted Nikon Eclipse 2000 ever, HS can do the screening and/or training of lab personnel microscope with bright-field, fluorescence and hyperspectral when needed. A hybridoma project usually requires three to five separation with two digital cameras. Workstation software is months for completion. The following timetable is used for gen- designed to automate image acquisition. The fluorescent system eral planning of a hybridoma project: on the workstation has a wide range of customized fluorescent filter cubes and microscope objectives. The Optical Insights hy- • Mouse immunization — 4-6 weeks perspectral system is designed specifically to eliminate problems • Selection of primary hybridomas after cell fusion — 2 weeks inherent in fluorescence microscopy. This system allows spectral • Expansion and freezing of primary hybridomas — 2 weeks separation of defined objects in the data set of imaged specimens. • Cloning and screening of clones — 2 weeks This system includes software for hyperspectral data acquisition • Expansion and freezing of clones — 2 weeks and analysis, in addition to MetaMorph (7.0) imaging software • Bioreactor/ascites fluid production — 4 weeks which allows 4D viewing and 3D measurements. Polyclonal antibody production is available in mice and rats for Molecular Printer System – Nano eNabler™ both on-campus and off-campus clients and in rabbits for on- The Nano eNabler™ system is a highly flexible molecular printer campus clients only. Polyclonal services include the purchase and that can dispense minute volumes of liquid at defined positions to care of animals through the Laboratory Animal Resource group create patterns of spots or lines with high spatial accuracy. This on campus, blood collection and processing of sera, adjuvant ad- technology enables the deposition of 1-30 µm sample droplets dition and injection of antigen, and administration of procedures quickly and precisely using materials such as proteins, nucleic according to an approved protocol and timetable. acids, lipids, colloids, quantum dots, UV-curable adhesives, etchants, and catalysts. Additional Equipment:

Laser Capture Microdissection System – LCM • Nine-cubic-foot, -80°C freezer Laser capture microdissection is a system used to identify and • Two, 18-foot ultra low (cryogenic) freezers for long-term retrieve individual cells from tissue sections. The retrieved cells storage of biological materials can be used for assessment/analysis of RNA, DNA, protein and • Eight tissue culture incubators other biochemical properties. Hybridoma Services and users will work together to develop Instruction, Training and Assistance an estimate for an individual project based on current pricing. An Individual Module Training Program (IMTP) trains individu- Project cost estimates are available at hybridoma.biotech.iastate. als to specifically process and visualize their research materials edu. Current price information for each procedure offered may be using targeted methods and instrumentation to accomplish their obtained by calling 515-294-9837 during regular business hours objectives. This program allows an individual to begin the IMTP or by sending an e-mail message to [email protected]. within a short time after an initial consultation and acceptance of the training conditions. Costs for IMTP include technical training More information is available online at http://hybridoma.biotech. assistance and instrument, lab and materials fees. The length of an iastate.edu. IMTP depends on the individual’s abilities and aptitudes and the complexity of the training. Individuals should contact the HRMF Macromolecular X-ray staff for further details and/or to schedule a consultation. Crystallography Facility More information is available online at http://microscopy.biotech. Location: 0202 Molecular Biology Building iastate.edu. Hours: 9 a.m. to 5 p.m. weekdays, by appointment Contact: Charles Stewart, Manager, 515-294-2846 Web: xray.biotech.iastate.edu Hybridoma Services The Macromolecular X-ray Crystallography Facility of the Office Location: 1106 (Lab) or 1104 (Office) of Biotechnology provides a Rigaku MicroMax-007HF rotat- Molecular Biology Building ing anode generator for single-crystal x-ray diffraction studies, Hours: 8 a.m. to 5 p.m. weekdays, by appointment stereomicroscopes for viewing crystals, temperature and vibration Contact: Amanda Brockman, Assistant Scientist controlled incubators for crystallization, computers with crystal- [email protected], lography software and training in crystallography techniques. 515-294-9837 Additionally, the facility arranges synchrotron data collections and Web: hybridoma.biotech.iastate.edu provides tools for sample prep and shipping. Facility services are provided on a fee-for-service basis and include crystal screen- Hybridoma Services (HS) of the Office of Biotechnology provides ing, data collection, data processing, and structure determination valuable resources for scientists who need monoclonal or poly- and analysis. The facility provides free consultations on protein clonal antibodies but do not have the appropriate equipment purification, crystallization strategy as well as letters of support or are not experienced in antibody production techniques. All and assistance with grant writing. The following outline is used hybridoma services are open to Iowa State University clients and for general planning of a crystallography project: off-campus individuals or companies. 1. Protein purification: Concentrated protein > 95% pure A wide array of procedures can be customized to meet individual 2. Crystal screening: Search for crystallization “hits” requirements. These techniques are provided on an individual 3. Crystal optimization: Generation of well-diffracting crystals charge basis and include animal immunization, cell fusion and from screening hits hybridoma culture maintenance, cell culture and maintenance of 4. Data collection: Using facility or synchrotron equipment other cell lines used in biotechnology and virology labs, large- 5. Structure determination: Processing, phasing, refinement, scale mammalian cell culture (bioreactor), blood sera collection, modeling, analysis and deposition antibody purification and isotyping, cryopreservation and cryostorage of cell lines (-140°C), and ELISA tests. The hybridoma More information is online at: www.xray.biotech.iastate.edu. 69 Research in Biotechnology 2020 X-ray Diffraction (XRD) Materials Analysis and The lab operates a Siemens D500 x-ray diffractometer that is Research Laboratory equipped with a diffracted beam monochromator and a sample spinner. It is also equipped with a heating stage to permit exami- Location: 23 Town Engineering nation of samples up to 300°C. Hours: 8 a.m. to 5 p.m. weekdays, other hours by appointment The diffractometer is fully computer-controlled using hardware Contact: Warren Straszheim, Manager, wesaia@iastate. and software from MDI, and the user has access to a wide variety edu, 515-294-8187 of Windows-based data reduction and analysis software. Programs Web: Marl.iastate.edu include algorithms for search-matching, quantitative analysis, profile fitting, calculating XRD patterns from known or approxi- The Materials Analysis and Research Laboratory (MARL) is a core mated crystal data, and the determination of crystallite size. Both facility of the Office of Biotechnology at Iowa State University. copper and chromium x-ray tubes are available. MARL’s function is three-fold: education, research and development, and service. Its facilities are used for chemical and physical X-ray Photoelectron Spectroscopy (XPS) characterization of a wide variety of materials to support research XPS is also sometimes known as Electron Spectroscopy for programs within the university. MARL also conducts research on Chemical Analysis (ESCA). It is a non-destructive technique for unusual material evaluation problems for outside agencies. (For analyzing the surface chemistry of a solid material. Samples are imaging biological samples, please contact the High Resolution excited using either Al or Mg x-rays. Electrons are emitted from Microscopy Facility.) the sample and their kinetic energy is measured to determine the binding energy of the electrons in the sample. The resolution MARL’s major equipment is listed below. is such that it is possible to determine the chemical state of the electrons in the sample. Scanning Electron Microscopy (SEM) MARL’s FEI Quanta-FEG 250 is a field-emission SEM with 1-nm The XPS is equipped with a cassette for loading up to ten samples resolution. It has a plasma cleaner for removing contamination at a time. The lab also has an exchange device for conveying that otherwise prevents the characterization of nano materials. single samples from a glovebox into the analyzer. The SEM also has environmental capabilities (E-SEM). It features a Peltier stage with moderate heating and cooling capabilities The system is equipped with a high speed ion gun for quickly (-25°C to 55°C). That permits examination of moist samples milling samples with minimal damage. This permits depth under saturated water vapor. It also features a heating stage used profiling to determine chemistry as a function of depth. to perform in situ analysis of samples in a temperature range from ambient to 1000°C. The Quanta is able to record movies in real X-ray Fluorescence (XRF) time. Bulk elemental analysis is provided by a PANalytical PW2404 x-ray fluorescence spectrometer. The spectrometer is equipped A beam deceleration option enables adjustment of the landing with a 60-specimen sample changer for automated operation. The energy of the electron beam on conductive samples. This option spectrometer utilizes a rhodium x-ray tube and a 4kW generator lowers the low kV limit to less than 100V and results in improved to provide the primary x-ray beam. This provides excellent sen- imaging at low voltage. sitivity for the determination of all elements from boron through uranium. The spectrometer is fully computer-controlled and The microscope has a variety of secondary electron and back- the user has access to both qualitative and quantitative analysis scattered electron detectors and a motorized stage. software. Specimens can be introduced into the spectrometer as bulk solids (powders, pellets or fused disks) or as liquids or X-ray Analysis for Microcharacterization (EDS & WDS) semi-solids. A full range of XRF sample preparation equipment is The SEM is equipped with an Oxford Aztec energy-dispersive also available at the lab, including an Eagon-2 automated sample spectrometer (EDS) for elemental analysis on a microscopic scale. fusion system. The EDS has a light-element detector for analysis of elements 2 as light as beryllium. The detector has an active area of 80-mm Thermal Analysis (TGA and SDT) (8 times larger than most EDS detectors) to achieve good count Thermal analysis is provided with TA Instruments thermogravi- rates, even at low beam currents. metric (TGA) and simultaneous DTA-TGA (SDT) modules. TGA can test samples in inert gases from ambient to 1200°C, with a 25 Software is provided for performing quantitative analysis, digital position auto-sampler. SDT can test samples in inert gases from imaging, line-scan x-ray profiles, and elemental x-ray maps. The ambient to a maximum of 1500°C. MARL software is unique in being able to correct line profiles and maps for effects from peak overlaps and background. Light Microscopy Light microscopes are available for reflected light (Olympus BH), MARL recently installed an Oxford wavelength-dispersive spec- transmitted light (Olympus BH-2) and for stereo (Olympus SZH) trometer (WDS) for analysis of difficult samples. WDS provides imaging. A digital camera can be mounted to any of the micro- better resolution than EDS for analysis of overlapping peaks. It scopes for recording still images or movies. also has a better peak-to-background ratio which improves the detection limit. Sample Preparation The lab is equipped with extensive facilities for preparing samples. Equipment includes cutoff saws, grinders, polishers, sputter coaters, evaporators, sample presses, etc. An ion mill is available for preparation of planar and cross sections for difficult samples. More information is available online at www.marl.iastate.edu.

70 Services and Facilities Analysis of samples for external researchers is offered for a fee. Materials Preparation Center After training, internal researchers may choose to analyze their Location: Metals Development Building own samples. Hours: 7:30 a.m. to 4 p.m. weekdays Contact: Matthew Besser, Director, [email protected], 515-294-5236 W. M. Keck Microfabrication Facility Web: www.ameslab.gov/mpc Location: 2831 Gilman Hall (Keck Lab) Hours: Mondays 1-3 p.m. or by appointment. Access The Materials Preparation Center (MPC) is a U.S. Department of available 6 a.m. to 10 p.m. after completion of Energy, Office of Science, Office of Basic Energy Sciences, Division training of Materials Sciences and Engineering Specialized Research Center located at the Ames Laboratory. The W. M. Keck Facility for the Fabrication of Microminiaturized Analytical Instrumentation (Keck Lab), a part of Chemical Ser- The MPC is recognized world-wide by the research community vices, provides Iowa State with access to microfabrication tech- for its unique capabilities in preparation, purification, single nologies. With its approximately 1,000 square feet of class 10/100 crystal growth and characterization of rare earth metals, alkaline- clean rooms, the Keck Laboratory supports all phases of micro- earth metals and refractory metal materials. MPC operations are fabrication and its use in fields ranging from analytical chemistry available on a full cost-recovery basis. to cell biology. Drawing from affiliates across campus and its resident support staff, expertise in micromechanics, microfluidics, Established in 1981, the MPC is a one-of-a-kind facility that is microchip arrays, biology, chemistry, physics and microelectronics acutely sensitive to the needs of researchers. The facility provides can be integrated in translating research ideas into experimental research and developmental quantities of high-purity materials reality. and unique characterization services to scientists at university, industry and government facilities on a cost-recovery basis, Research capabilities in the laboratory include developing mi- allowing access to novel materials and new technologies as they croanalysis systems, chip-scale chromatography, microelectrode are developed. assemblies, biochips and cell culture platforms. The laboratory currently houses equipment for optical lithography, surface Renowned for its outstanding technical expertise, the MPC’s al- metrology (stylus profilometry), wet chemical etching, and plasma loy preparation and processing capabilities are of great potential etching. The facility will soon expand to include wire bonding, value to America’s advancing technology. The MPC provides the thin film deposition and dry chemical etching capabilities. personal service required to meet each client’s individual needs. Customized materials and services that are not available from commercial vendors, and are unmatched in quality anywhere else in the world, are provided by the MPC. The range of utilization is demonstrated by the scientific publications in which the MPC and Food Science and Human Nutrition’s Ames Laboratory are cited. Nutrition and Wellness Equipment and services information is available on the MPC Research Center website, www.ameslab.gov/mpc. Location: 2325 North Loop Drive, Suite 6100 (Iowa State Research Park) and 1126 Human Nutritional Sciences Building (Sensory Evaluation Unit) W. M. Keck Metabolomics Hours: 8 a.m. to 5 p.m. weekdays and as arranged Contact: Ruth MacDonald, Interim Laboratory Director Research Laboratory [email protected] Location: 0124 Molecular Biology Building (W. M. Keck Research Laboratory) The Nutrition and Wellness Research Center (NWRC) is equipped 0101 Molecular Biology Building to facilitate research projects involving human subjects, featuring (FT-ICR Mass Spectrometry) a variety of research, laboratory space and equipment for con- Hours: Weekdays by appointment trolled studies that involve diet, exercise and behavior change. Contact: Ann Perera, Manager, metabolomics@iastate. Equipment may be used by researchers to collect data on meta- edu. 515-294-3019 bolic risk factors, body composition and/or physical fitness. Web: www.metabolomics.biotech.iastate.edu An exercise area with private locker room and shower facilities provides convenient space for controlled exercise studies. Exercise The W. M. Keck Metabolomics Research Laboratory houses many equipment includes several treadmills, three cycle ergometers and different analytical platforms, including Agilent gas chromatogra- metabolic carts. phy instruments with EI and CI ionization methods, GC-GCMS and GC-MS heart cut; an Agilent ion-trap LC/MS instrument with Facilities are available for pilot and research studies. Individual ESI, APCI and APPI ionization capabilities; Agilent LC-TOF mass testing rooms are available to conduct a variety of clinical mea- spectrometer; JEOL TOF mass spectrometer with DART source; surements. One room is set up with a metabolic cart and can be Agilent 1100 and 1260 HPLC systems with UV-DAD, fluores- used for assessing resting and exercise-related energy expenditure. cence and ELSD detectors; and a Synergy Two multi-mode micro- Another room is designated for body composition via BODPOD, plate reader with the ability to assay optically-active molecules via PeaPod, bioelectrical impedance analysis (BIA) and bone mineral luminescence, fluorescence, fluorescence polarization, and UV-Vis densitometry via dual-energy x-ray absorptiometry (DXA). An absorbance (monochromator-based). adjacent room is available for anthropometry assessment. Two phlebotomy rooms are available for collecting blood and other The laboratory has expanded to include a Bruker Solarix FT-ICR clinical data. A fully-equipped metabolic kitchen with a profes- instrument, located in 101 Molecular Biology Building. FT-ICR is sional stove/oven, microwave ovens, two dishwashers, refrigera- a high-end mass spectrometer that provides ultra-high-accuracy tor/freezer, walk-in pantry, walk-in cold room, walk-in freezer mass measurements to sub-ppm level. The laboratory is now and an ice machine are available. Adjacent dining rooms provide capable of providing MALDI-mass spec imaging. capacity to serve meals for up to 40 people simultaneously. Ad- ditionally, the on-campus facility has a fully equipped kitchen, as 71 Research in Biotechnology 2020 well as a dining room with the capacity to serve meals for 24-40 Domestic pet urine and feces participants simultaneously, a phlebotomy and anthropometric The labs have expertise in state-of-the art chemical and sensory room and a private room for clinical counseling/interviewing. analysis of odorous compound analysis of urine and feces of domestic pets like cats and dogs. Chemical method detection limits Estimates regarding costs to conduct human clinical studies are are between part-per-billion and part-per-quadrillion levels. available by contacting the department. Waste Management The labs have expertise in livestock and municipal waste man- Sensory Evaluation Unit agement, valorization of biomass, torrefaction, waste-to-carbon, waste-to-energy and circular economy. The Sensory Evaluation Unit (SEU) provides facilities and expertise for the sensory evaluation of food products and ingredients. Wine flavor, ethanol, aroma and nutraceuticals The labs specialize in state-of-the art chemical and sensory analy- The unit’s facilities include a computerized ten-booth testing area, sis of wine flavors, ethanol impurity, aromas and nutraceuticals. a panelist training room, a sample preparation area and an adjoin- Chemical method detection limits are between part-per-billion ing food science and human nutrition laboratory equipped for and part-per-quadrillion levels. measuring food color, texture and viscosity. Thermal desorption multidimensional GC-MS-olfactometry Services include sensory project design and management; panelist The unique TD-MDGC-MS-O system includes a custom-made recruiting, screening, and training; and collection, analysis and GC-MS system with heart-cutting capability based on a Dean interpretation of data. switch capable of simultaneous chemical and livestock odorants analyses. The instrument is configured to analyze volatile organ- Analytical tests available include discrimination tests, descrip- ics as gas samples or from solid samples with sorbent tubes and tive analysis and time intensity. Consumer tests include product SPME. preference and acceptance. Fees are based on the actual costs for supplies, labor, the use of Solid phase microextraction (SPME) the facilities and panelist incentives. For estimates, please contact SPME is used for extractions of gases emitted from a variety of the facility at 515-294-4323. samples, including livestock and poultry manure, air, food, bever- ages, packaging, bio-based products, live insects, plants, breath and general applications related to quality control and quality assurance.

Olfactometry and Air Quality Laboratories Non-destructive in vivo sampling and analysis of volatiles The labs are equipped for technologies for non-destructive in vivo Location: 0205, 3214 and 4216 Sukup Hall sampling and analysis of volatiles from insects, plants, fruits and Hours: Professional assistance available 8 a.m. to 5 microbial cultures and tissues. p.m. weekdays. Contact: Jacek Koziel, Professor-in-Charge, More information is available online at www.biotech.iastate.edu/ [email protected], 515-294-4206 biotechnology-service-facilities/atmospheric-air-quality-laboratory/. The Olfactometry and Air Quality Laboratories of the Department of Agricultural and Biosystems Engineering are situated in Sukup Hall and serve as a focal point for collaborative research and training in air quality engineering and livestock odor. The laboratories Plant Transformation Facility specialize in development and testing of odor and gaseous emissions mitigation technology, continuous air quality monitoring, Location: B0423 and B0426 Agronomy Hall environmental analysis, quantification of organic compounds with Hours: 8 a.m. to 5 p.m. weekdays, by appointment gas chromatography and mass spectrometry, and simultaneous Contact: David Wright, Manager, [email protected] chemical and olfactometry analyses. 515-294-5415

Livestock odor and gaseous emissions mitigation The Iowa State University Plant Transformation Facility offers The labs offer lab-scale and pilot-scale development and testing of corn, soybean and rice transformation services for on- and off- odor and gaseous emissions mitigation based on physical, chemi- campus research scientists. Transgenic materials are available cal and biological approaches. Testing of the treatment as callus (corn and rice), plantlets (corn, soybean and rice) and efficacy targeting odor, ammonia, hydrogen sulfide, volatile seed (corn and soybean). Transformed corn, soybean and rice are

organic compounds, and greenhouse gases (CO2, CH4, N2O) is generated using Agrobacterium tumefaciens-based methods, while conducted. Full scale tests are conducted at university and com- biolistic-based transformation is available for corn. mercial farms. The facility also provides instrumentation for on-campus person- Air quality monitoring for odor, gases, particulate matter nel and provides technical assistance, consultation and training and greenhouse gases at livestock operations for on- and off-campus research scientists. The facility is sup- The laboratories are equipped with a variety of real-time analyzers ported by client-based fees, the faculty and staff of Iowa State for gaseous ammonia, hydrogen sulfide, carbon dioxide, methane, University and the Office of Biotechnology. nitrous oxide, volatile organic compounds, particulate matter and ozone. They are also equipped to conduct field air sampling and Fee-for-service information is available online at http://www. analysis of organic gases with sorbent tubes and SPME. biotech.iastate.edu/ptf/.

72 Services and Facilities Protein Facility Peptide Synthesis The facility can do both large- and small-scale peptide synthesis, Location: 1178 Molecular Biology Building including the synthesis of phosphopeptides, peptides contain- Hours: 7 a.m. to 5 p.m. weekdays ing unusual amino acids and Multiple Antigen Peptide Systems Contact: Joel Nott, Manager, [email protected], (MAPS) for vaccine production or monoclonal antibody produc- 515-294-3267 tion. The facility also has the capability to synthesize combinato- Web: www.protein.iastate.edu rial peptide libraries.

Protein/Peptide Sequencing The Protein Facility provides equipment and expertise for the The facility provides N-terminal protein/peptide sequence analysis analysis, characterization and synthesis of proteins and peptides of samples in solution or of samples electroblotted onto polyvi- and is open to both internal and external investigators. The nylidene difluoride (PVDF) membrane. The facility personnel also Protein Facility is supported by the Office of Biotechnology, the perform chemical and enzymatic digestion of proteins in solu- Roy J. Carver Department of Biochemistry, Biophysics and tion or proteins blotted onto PVDF to provide internal sequence Molecular Biology and the Plant Sciences Institute. The following information. instrumentation is available in the facility: Tandem Mass Spectrometry (LC-MS/MS) • Amersham Pharmacia DALT 2D electrophoresis system The facility has a Q Exactive™ Hybrid Quadrupole-Obrbitrap • Amersham Pharmacia Image Scanner with transparency Mass Spectrometer from Thermo Scientific. This is an LC-MS/MS module system combining quadrupole precursor ion selection with high- • Beckman System Gold high-performance liquid chromato- resolution, accurate-mass Orbitrap detection. The system can be graphs coupled with either an Agilent 1260 Infinity Capillary pump with x Model 125A Analytical Solvent Module with Model 166 thermostatted column compartment and autosampler or a Ther- programmable UV detector moScientific EASY-nLC 1200 nanopump with integrated autos- x Model 125S Microanalytical Solvent Module with Model ampler. Both a Thermo Scientific API Source with a HESI-II Probe 166 programmable UV detector and SC100 fraction col- and a Thermo Scientific NanoSpray Flex Ion source are available. lector • Beckman Coulter P/ACE MDQ Capillary Electrophoresis This instrument can be used for a variety of experiments, System including the following: • Biorad minigel and blotting apparatus • C.B.S. Scientific spot-picking workstation with UV lightbox • Intact mass measurement: The protein of interest is isolat- • Denovix DS-11 Fx+ ed using chromatographic techniques and introduced to the • GE Healthcare Ettan IPGPhor 3 IEF unit mass spectrometer. The protein can be subjected to a variety • GE Healthcare Typhoon FLA 9500 Scanner of fragmentation techniques (CID and HCD). The protein’s • Genomics Solutions ProGest molecular weight can be determined by deconvolution of the • Savant SpeedVac Plus fragment masses from the various fragmentation techniques. • Shimadzu Axima Confidence MALDI-TOF • Protein ID: For both gel-based and chromatographic sepa- • Shimadzu PPSQ-53A Protein/peptide sequencer rations, proteins will be identified, when possible, by search- • Thermo Scientific Q Exactive Hybrid Quadrupole-Orbitrap ing databases with both MS and MS/MS data using Mascot Mass Spectrometer coupled with either of the following: software. For organisms which do not have complete protein x Agilent 1260 Infinity capillary pump with thermostatted databases, de novo sequences from trypsin fragments can be column compartment autosampler obtained from MS/MS data. Digestion with other proteases x Thermo Scientific EASY nLC-1200 nano-LC pump with is available to increase coverage when required. MS-based integrated autosampler methods are also available for the identification and mapping • Virtis Model 3.5L DBTZL Benchtop FreezeDryer (lyophi- of post-translational modification of proteins. A data analysis lizer) workstation containing the programs required for the inter- • Waters Synapt G2-Si with ACQUITY H-Class UPLC and pretation of mass spectra and database searching is available ACQUITY M-Class UPLC with HDX manager in the facility. Data analysis can be conducted by individual • Software for the analysis of 1D and 2D gels investigators, with assistance and training from facility staff. • Label-free relative quantification of complex protein The following services are offered: extracts: Crude protein extracts are digested in solution with trypsin/Lys-C. After digestion, 250 fmol of PRTC stan- High Performance Liquid Chromatography (HPLC) dard is spiked into the sample to serve as an internal control. The facility offers microanalytical, analytical and semi-preparative The peptides are then separated by liquid chromatography HPLC purification of proteins and peptides. The HPLCs also are and analyzed by MS/MS by fragmenting each peptide. The available as user-operated instruments after a required training resulting intact and fragmentation pattern is compared to a session conducted by facility personnel. theoretical fragmentation pattern (from either MASCOT or Sequest HT) to find peptides that can be used to identify the MALDI-TOF Mass Spectrometry proteins. Label-free quantification uses the Minora Feature The facility provides mass spectrometry services for proteins, Detector to detect and quantify isotopic clusters. peptides, glycoproteins, oligosaccharides, oligonucleotides and • TMT: Crude protein extracts are digested in solution with other polymers using a matrix-assisted laser desorption/ioniza- trypsin/Lys-C. After digestion, the samples are grouped into tion time-of-flight (MALDI-TOF) mass spectrometer. The mass sets of ten and labeled with TMT reagents. A control sample spectrometer also is available as a user-operated instrument after a (composed of an aliquot of all of the samples in the sample required training session. set) is also labeled with an isobaric tag. This control sample is pooled with the ten TMT-labeled samples so that multiple samples can be quantified in a single run. The peptides are then separated by liquid chromatography and analyzed by MS/MS by fragmenting each peptide. The peptides from multiple samples are chemically identical and isobaric so they 73 Research in Biotechnology 2020 will co-elute during the LC run. When they are subjected to Isoelectric Focusing (IEF) HCD fragmentation, the tags from each individual sample The facility provides IEF as a method for separating proteins will generate a unique low-mass reporter ion. The resulting based on isoelectric point prior to SDS-PAGE. The IEF equipment intact and fragmentation pattern is compared to a theoreti- also is available as a user-operated instrument after a required cal fragmentation pattern (from either MASCOT or Sequest training session, allowing researchers to perform the second di- HT) to find peptides that can be used to identify the proteins. mension in their own labs. Reporter ions intensities can then be used for quantification purposes. 2-D Gel Analysis SameSpots from Nonlinear Dynamics allows for the alignment SYNAPT G2-Si HDMS and analysis of 2-D gels in a short amount of time and returns The SYNAPT G2-Si High Definition Mass Spectrometer from data that shows the expression of proteins under different biologi- Waters is a hybrid-quadrupole, ion-mobility, orthogonal-accelera- cal conditions. The software presents a list of the most signifi- tion time-of-flight (oa-ToF) mass spectrometer that combines high cantly changing spots within a set of gels, allowing for the rapid resolution mass spectrometry with high-efficiency ion mobility- screening of the protein spots that may be of the most interest in based measurements and separation (IMS). It is fitted with a dual- a set of gels. These spots can then be further analyzed by other orthogonal API source (LockSpray™ ESI/APCI). Also available equipment in the Protein Facility (trypsin digestion followed are a Waters ACQUITY M-Class UPLC system with Hydrogen- by peptide mass fingerprinting or internal sequencing, direct Deuterium Exchange (HDX) technology and a Waters ACQUITY N-terminal sequencing of the blotted gels or MS/MS using the Q H-Class UPLC system. Exactive tandem mass spectrometer). This package has the ability to align the gels for complete spot matching. SameSpots is also Enzyme Digestion available for individual use. The facility provides in-gel digestion of protein samples from 1D or 2D gels. Gel spots can be digested with a variety of enzymes, Image Scanning and Analysis including trypsin, Arg-C and Glu-C. The resulting peptides from The Typhoon FLA 9500 is a multimode laser scanner for imaging the digestion can then be analyzed by MS or MS/MS methods. and analyzing proteins, DNA and other biomolecular samples. The peptides from the digested proteins can also be separated by The system is capable of imaging gels, membranes and plates HPLC for further analysis by N-terminal sequencing or MALDI- produced by a variety of techniques: multifluorescent, chemilu- TOF. A UV lightbox and spot picking tools are available for minescent, radio-labeled (phosphor screens), infrared dyes and manual gel processing. Digestion of samples in solution can also common visible stains such as silver and Coomassie Blue. The be performed. instrument also is equipped with a large scan area (40 x 46 cm) that allows for the scanning of large 2-D gels and multiple gels or SDS-PAGE/Electroblotting blots simultaneously. The facility provides SDS-PAGE analysis of proteins for purity and molecular weight estimation and western blotting to nitrocellulose More information is available online at www.protein.iastate.edu. or to PVDF for immuno-detection and protein/peptide sequencing, respectively. Stained gels (Sypro Ruby®, Coomassie Brilliant Blue, silver, etc.) can be scanned and analyzed in the facility. The SDS-PAGE equipment also is available as a user-operated service after a required training session.

2-D Gel Electrophoresis The facility provides two-dimensional electrophoresis by separating proteins in the first dimension according to charge [isoelectric focusing (IEF)], followed by separating the focused proteins in the second dimension according to molecular weight by SDS-PAGE. The proteins may be visualized by staining with Coomassie Bril- liant Blue R250, silver stain or fluorescent dyes. These spots can be excised for further analysis, or the 2D array can be analyzed for differences in protein quantity or in proteins present in the gel. The 2D gels also can be electroblotted to PVDF or nitrocellulose membranes for further analysis. The 2D electrophoresis equipment also is available as a user-operated service after a required training session.

74 Research in Biotechnology 2020 Faculty Areas of Interest If you are searching for a researcher in a particular area of interest, the Office of Biotechnology's online database of researchers at www.biotech.iastate.edu may be a useful resource.

Faculty Indexed by Departments Asterisk indicates appointment in more than one department

Agricultural and Hansen, Stephanie L. Biomedical Sciences Civil, Construction Biosystems Engineering Keating, Aileen F. Anantharam, Vellareddy and Environmental Brown, Robert C.* Kerr, Brian J. Beck, Josh Engineering Brumm, Thomas J. Koltes, James Bracha, Vlastislav Ellis, Timothy G. Helmers, Matt Lamont, Susan J. Carlson, Steve Ikuma, Kaoru Howe, Adina Lippolis, Katy Charavaryamath, Ong, Say Kee Hurburgh, Jr., Charles R. Lonergan, Elisabeth Chandrashekhar Kanwar, Rameshwar S. Lonergan, Steven Cho, Michael W. Computer Science Koziel, Jacek Putz, Austin Day, Tim A. Chang, Carl K. Maier, Dirk Reecy, James Greenlee, Heather Ciardo, Gianfranco Peschel, Joshua Ross, Jason W. Kanthasamy, Anumantha Eulenstein, Oliver Raman, D. Raj Rothschild, Max F. Kanthasamy, Arthi Fernandez-Baca, David Rosentrater, Kurt A. Schmitz-Esser, Stephan Kim, Jonoh Huang, Xiaoqiu Soupir, Michelle Sebranek, Joseph G.* Kimber, Michael J. Lathrop, James Steward, Brian L. Selsby, Joshua Mair, Gunnar Li, Qi Yu, Chenxu Serão, Nick VL Martin, Richard Lutz, Jack H. Stabel, Judith R. Mochel, Jonathan Paul* Lutz, Robyn R. Aerospace Engineering Tuggle, Christopher K. Singh, Ravindra Miller, Les Kim, A. Ram Wolc, Anna Thippeswamy, Thimmasettappa Miner, Andrew S. Youngs, Curt Verma, Saurabh Agronomy Ware, Wendy* Ecology, Evolution and Al-Kaisi, Mahdi Roy J. Carver Department Organismal Biology Beavis, William of Biochemistry, Chemical and Biological Bronikowski, Anne Becraft, Philip W.* Biophysics and Molecular Engineering Hufford, Mathew B. Bhattacharyya, Madan K. Biology Bratlie, Kaitlin* Janzen, Frederic J. Edwards, Jode Ambrosio, Linda Brown, Robert C.* Lavrov, Dennis Gardner, Candice Andreotti, Amy H. Cochran, Eric W. Lu, Chaoqun Goggi, A. Susana Beitz, Donald C.* Hillier, Andrew Most, Corinna Grant, David Bobik, Thomas Jarboe, Laura Nason, John Knapp, Allen Chen, Stone Mallapragada, Surya K. Serb, Jeanne Lamkey, Kendall R. DiSpirito, Alan A. Mansell, Thomas Severin, Andrew Lawrence-Dill, Carolyn* Girton, Jack R. Narasimhan, Balaji Toth, Amy L. Lee, Michael Hargrove, Mark Reuel, Nigel Valenzuela, Nicole Licht, Mark A. Honzatko, Richard B. Schneider, Ian C. Wendel, Jonathan F. Lübberstedt, Thomas Jernigan, Robert Shanks, Brent Moorman, Thomas B. Johansen, Jorgen Shanks, Jacqueline V. Economics Salas Fernandez, Maria Johansen, Kristen Shao, Zengyi Gallagher, Paul W. Schnable, Patrick S. MacIntosh, Gustavo Tessonnier, Jean-Phillippe Hayes, Dermot J. Scott, Paul Miller, W. Allen* Vigil, Dennis R. Huffman, Wallace Singh, Asheesh K. Moss, Walter Wang, Qun Jacobs, Keri L. Wang, Kan Myers, Alan M. Wu, Yue Lence, Sergio Yu, Jianming Nelson, Scott W. Moschini, GianCarlo Nikolau, Basil J. Chemistry Animal Science Nilsen-Hamilton, Marit Anand, Robbyn Baumgard, Lance Peters, Reuben Gordon, Mark Beitz, Donald C.* Rau, Guru Jenks, William Bobeck, Elizabeth Roche, Julien Kraus, George A. Cunnick, Joan E. Sashital, Dipali G. Lee, Young-Jin Dekkers, Jack C. M. Shogren-Knaak, Michael Petrich, Jacob W. Dickson, James S. Thornburg, Robert W. Potoyan, Davit Ellinwood, N. Matthew Underbakke, Eric S. Smith, Emily Fernando, Rohan L.* Zabotina, Olga Song, Xueyu Gabler, Nicholas Stanley, Levi Greiner, Laura VanVeller, Brett Venditti, Vincenzo 75 Faculty Indexed by Department Electrical and Computer Vollbrecht, Erik Philosophy and Religious Karriker, Locke Engineering Wurtele, Eve Syrkin Studies Li, Ganwu Bigelow, Timothy A. Yandeau-Nelson, Marna Tsou, Jonathan Y. Linhares, Daniel Dickerson, Julie A. Yin, Yanhai Wolf, Clark Mochel, Jonathan Paul* Dong, Liang Opriessnig, Tanja Huang, Cheng Geological and Physics and Astronomy Pineyro, Pablo Jiles, David C. Atmospheric Sciences Sivasankar, Sanjeevi Plummer, Paul K. Khokhar, Ashfaq Swanner, Elizabeth Travesset, Alex Ramirez, Alejandro Kim, Jaeyoun Wang, Xuefeng Sahin, Orhan Lu, Meng Horticulture Sato, Yuko Neihart, Nathan M. Arora, Rajeev Plant Pathology and Wang, Chong Pandey, Santosh Fei, Shui-zhang Microbiology Yoon, Kyoung-Jin Que, Long Hannapel, David J. Arias, Silvina Zhang, Jianqiang Zheng, Mai Baum, Thomas J. Zimmerman, Jeffrey J. Human Development and Beattie, Gwyn A. Entomology Family Studies Gleason, Mark L. Veterinary Microbiology Coats, Joel R. Shirtcliff, Elizabeth Halverson, Larry and Preventive Medicine Gassmann, Aaron J. Lauter, Nick Allen, Heather K. Hellmich, Richard L. Industrial and Leandro, Leonor Bannantine, John P. Jurenka, Russell A. Manufacturing Systems Miller, W. Allen* Bellaire, Bryan O’Neal, Matthew Engineering Munkvold, Gary Blitvich, Bradley J. Sappington, Thomas W. Li, Qing Robertson, Alison Friedberg, Iddo Smith, Ryan C. Tylka, Gregory L. Kreuder, Amanda Kinesiology Walley, Justin Kudva, Indira T. Food Science and Human Chou, Li-Shan Whitham, Steve Looft, Torey Nutrition Derrick, Timothy R. Wise, Roger P. Loving, Crystal Boylston, Terri D. Gillette, Jason C. Yang, X. B. Lyte, Mark Brehm-Stecher, Byron Kohut, Marian L. Maury, Wendy Lamsal, Buddhi Lang, James A. Political Science McGill, Jodi MacDonald, Ruth S. Lee, Duck-chul Shelley, Mack C.* Miller, Cathy L. McNeill, Elizabeth Sharp, Rick L. Wang, Yu Minion, Chris Mellata, Melha Stegemoller, Elizabeth Phillips, Gregory J. Mendonca, Aubrey F. Valentine, Rudy Statistics Roof, Michael Prusa, Kenneth J. Dai, Xiongtao Roth, James A. Reddy, Manju Materials Science and Dixon, Phillip Sacco, Randy Rowling, Matthew Engineering Dorman, Karin S.* Sponseller, Brett A.* Schalinske, Kevin L. Bratlie, Kaitlin* Dutta, Somak Stabel, Judith R. Sebranek, Joseph G.* Jiang, Shan Liu, Peng Vaughn, Eric M. Talbert, Joey Nettleton, Dan Verhoeven, David Watrelot, Aude Mathematics Niemi, Jared Wannemuehler, Michael White, Wendy S. Kadelka, Claus Qiu, Yumou Wu, Zuowei Willette, Auriel Parshad, Rana Shelley, Mack C.* Zhang, Qijing Wilson, Lester A. Smith, Jonathan D. H. Wu, Zhijun Veterinary Clinical Veterinary Pathology Genetics, Development Yan, Jue Sciences Andreasen, Claire B. and Cell Biology Allenspach, Karin Brewer, Matt Aung, Kyaw Mechanical Engineering Caston, Stephanie Haynes, Joseph S. Bai, Hua Bentil, Sarah A. Ellinwood, N. Matthew* Jelesijevic, Tomislav Bassham, Diane Brown, Robert C.* Ho Eckart, Louisa Jones, Douglas E. Becraft, Philip W.* Claussen, Jonathan Johannes, Chad Mooyottu, Shankumar Coffman, Clark Hashemi, Nastaran LeVine, Dana Smith, Jodi D. Dorman, Karin S.* Heindel, Theodore J. Palerme, Jean-Sebastien Espin Palazon, Raquel Juarez, Jaime J. Sebbag, Lionel Essner, Jeffrey J. Montazami, Reza Sponseller, Brett A.* Asterisks indicate Gu, Xun Pandalkar, Sonal Ware, Wendy* appointment in more than Gupta, Mohan Ren, Juan Zellner, Eric one department Henderson, Eric R. Shrotriya, Pranav Howell, Stephen H. Sundararajan, Sririam Veterinary Diagnostic Kelley, Dior and Production Animal Lawrence-Dill, Carolyn* Natural Resource Ecology Medicine McGrail, Maura A. and Management Baum, David H. Peterson, Thomas Adelman, James S. Clavijo, Maria J. Powell-Coffman, Jo Anne Blanchong, Julie A. Derscheid, Rachel J. Rodermel, Steven Roe, Kevin J. Gauger, Phillip Sakaguchi, Donald S. Halbur, Patrick G. Harmon, Karen M. 76