A New Tool for the Detection of Horsemeat Adulteration in Raw Meat
ors ens & B s io io e de Faria et al., J Biosens Bioelectron 2018, 9:4 B l e f c o t r l DOI: 10.4172/2155-6210.1000264 o Journal of a n n i r c u s o J ISSN: 2155-6210 Biosensors & Bioelectronics Research Article Open Access A New Tool for the Detection of Horsemeat Adulteration in Raw Meat Ricardo Adriano Dorledo de Faria1,2, Iden H2, Bharucha E2, Lins VFC1, Younès Messaddeq2,3*, Matencio T4 and Heneine LGD5 1Department of Chemical Engineering, Universidade Federal de Minas Gerais (UFMG), Brazil 2Center for Optics, Photonics and Lasers (COPL), Université Laval, Canada 3Institute of Chemistry, UNESP, Araraquara, São Paulo, Brazil 4Department of Chemistry, Universidade Federal de Minas Gerais (UFMG), Brazil 5Department of Applied Immunology, Fundação Ezequiel Dias (FUNED), Brazil Abstract This work deals with the development of an impedimetric immunosensor sensitive to horse immunoglobulin G. The biosensing platform involve the electrodeposition of polyaniline onto screen-printed carbon electrode as electrochemical transducer matrix. Cyclic Voltammetry and Scanning Electron Microscopy were used to monitor the formation of the recognition layer through the immobilization of anti-horse IgG antibodies on the conductive polymer. Electrochemical Impedance Spectroscopy (EIS) was performed to test the specific detection of horse serum in PBS buffer, indicating that the immunosensor was sensitive to the target analyte and without any response to heterologous sera (swine and bovine). The constructed sensors were first tested with PBS homogenates of commercial samples of raw ground horsemeat, pork and beef diluted at 10-5, 10-4 and 10-3% w/v in PBS.
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