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Musashi RNA-Binding Proteins As Cancer Drivers and Novel Therapeutic Targets Alexander E

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Musashi RNA-Binding Proteins As Cancer Drivers and Novel Therapeutic Targets Alexander E Published OnlineFirst January 31, 2017; DOI: 10.1158/1078-0432.CCR-16-2728 Review Clinical Cancer Research Musashi RNA-Binding Proteins as Cancer Drivers and Novel Therapeutic Targets Alexander E. Kudinov1, John Karanicolas1, Erica A. Golemis1, and Yanis Boumber1,2 Abstract Aberrant gene expression that drives human cancer can arise mRNA stability and translation of proteins operating in essential from epigenetic dysregulation. Although much attention has oncogenic signaling pathways, including NUMB/Notch, PTEN/ focused on altered activity of transcription factors and chroma- mTOR, TGFb/SMAD3, MYC, cMET, and others. On the basis of tin-modulating proteins, proteins that act posttranscriptionally these activities, MSI proteins maintain cancer stem cell popula- can potently affect expression of oncogenic signaling proteins. tions and regulate cancer invasion, metastasis, and development The RNA-binding proteins (RBP) Musashi-1 (MSI1) and Musashi- of more aggressive cancer phenotypes, including drug resistance. 2 (MSI2) are emerging as regulators of multiple critical biological Although RBPs are viewed as difficult therapeutic targets, initial processes relevant to cancer initiation, progression, and drug efforts to develop MSI-specific inhibitors are promising, and RNA resistance. Following identification of Musashi as a regulator of interference–based approaches to inhibiting these proteins have progenitor cell identity in Drosophila, the human Musashi proteins had promising outcomes in preclinical studies. In the interim, were initially linked to control of maintenance of hematopoietic understanding the function of these translational regulators may stem cells, then stem cell compartments for additional cell types. yield insight into the relationship between mRNA expression and More recently, the Musashi proteins were found to be overex- protein expression in tumors, guiding tumor-profiling analysis. pressed and prognostic of outcome in numerous cancer types, This review provides a current overview of Musashi as a cancer including colorectal, lung, and pancreatic cancers; glioblastoma; driver and novel therapeutic target. Clin Cancer Res; 23(9); 2143–53. and several leukemias. MSI1 and MSI2 bind and regulate the Ó2017 AACR. Introduction signaling cascade, a critical regulator of asymmetric cell division in cell progenitors (3). Further studies in Drosophila demonstrated The Drosophila musashi (msi) gene was first described in 1994 roles for msi in development of spermatogenesis and germline by Nakamura and colleagues (1) based on its role in regulating stem cells (4–6). asymmetrical division of Drosophila sensory organ precursor Following the identification of murine and human MSI1 (SOP) cells. Whereas SOPs normally undergo a program of cell (7, 8) and MSI2 (2), profiling of gene expression and direct division that culminates in production of neuronal precursors, a functional assessments in mouse models first demonstrated an bristle shaft and a socket cell, loss of msi leads to a double-bristle evolutionarily conserved role in guiding the appropriate dif- phenotype. The gene name reflects similarity of this phenotype to ferentiation of neuronal progenitor cells (7, 9, 10). This was martial portrayals of the two-sword fighting style originated by the followed by additional studies showing roles for the Musashi Japanese national hero Miyamoto Musashi. The msi gene is proteins in regulating normal cell differentiation and organ evolutionarily conserved: As a consequence of earlier gene dupli- development for other tissue types (5, 11–15), emphasizing the cation, humans have two related genes, Musashi-1 (MSI1) and essential nature of this protein signaling over great evolutionary Musashi-2 (MSI2). MSI1 and MSI2 share roughly 75% amino acid distance. For example, in early studies in mice, Msi1 was found identity in overall structure and similar to msi, belong to a family to be highly expressed in the brain, and Msi1 deficiency caused of RNA-binding proteins (RBP; ref. 2). Since their first identifica- hydrocephaly (16). MSI2 is a critical regulator of hematopoietic tion, the Musashi genes have attracted considerable interest as stem cells, where it plays a function distinct from MSI1 regulators of stem and progenitor cell characteristics. Subsequent (11, 12). Although MSI2 deletion leads to a decrease in study of msi demonstrated that it posttranscriptionally regulated hematopoietic stem cells, overexpression of MSI2 leads to an expression of genes such as numb, a component of the Notch increase in progenitor and stem cells (11, 12). MSI2 also regulates spermatogenesis and embryogenesis (4–6, 15). Cancer is a disease characterized by changes in cell symmetry, 1Program in Molecular Therapeutics, Fox Chase Cancer Center, Philadelphia, differentiation, and self-renewal capacity. Unsurprisingly, for a Pennsylvania. 2Department of Hematology/Oncology, Fox Chase Cancer Cen- number of genes originally identified as regulators of stem and ter, Philadelphia, Pennsylvania. progenitor cell capacity, altered function has been linked to Corresponding Author: Yanis Boumber, Molecular Therapeutics Program, cancer. The Musashi proteins were first linked to cancer in Department of Hematology/Oncology, Fox Chase Cancer Center, 333 Cottman 2001 based on studies demonstrating the elevated expression of Avenue, Philadelphia, PA 19111. Phone: 215-214-4297; Fax: 215-728-3639; E-mail: MSI1 in gliomas (17), medulloblastomas (18), and hepatomas [email protected] (19). In 2003, MSI2 was identified as part of a translocation event doi: 10.1158/1078-0432.CCR-16-2728 with HoxA9 in chronic myeloid leukemias (CML) that preserved Ó2017 American Association for Cancer Research. MSI2 RNA-binding motifs (20), also implicating this paralog in www.aacrjournals.org 2143 Downloaded from clincancerres.aacrjournals.org on October 1, 2021. © 2017 American Association for Cancer Research. Published OnlineFirst January 31, 2017; DOI: 10.1158/1078-0432.CCR-16-2728 Kudinov et al. Table 1. Summary of Musashi-1 and Musashi-2 signaling effectors Direction of Gene Target gene effect on target Protein function Cell type Reference Musashi-1/2 BRD4 " Receptor tyrosine kinase; cell invasion Pancreatic adenocarcinoma 23 cMET " Epigenetic controls; DNA repair HMGA2 " Notch ligand Jagged 1 # Notch antagonist Breast cancer 54 NUMB # Cell-cycle regulation Neural progenitor cells 3, 11 p21 (CDKN1A) # Receptor tyrosine kinase; cell invasion Embryonic kidney cells 37, 92 Intestinal epithelium Musashi-1 Doublecortin # Microtubule bundling Neural progenitor cells 90 Musashi-2 # Translation regulation Testis germ cells 6 NF-YA # Transcription factor Breast cancer 59 Tetraspanin 3 " Formation of supramolecular membrane complexes AML 36 Musashi-2 AHR " Transcription factor; aryl hydrocarbon receptor HSC 91 ESR1 " Hormonal receptor Breast cancer 30 Hoxa9 " Transcription factors; lymphocyte development ALL/AML 53 Ikzf2 " Myc " Transcription factor; cell-cycle progression PTEN # Phosphatase and inhibitor of PDK–AKT–mTORC1 Intestinal epithelium 37 LRIG1 # Negative regulator of tyrosine kinase signaling SMAD3 # Transmembrane threonine kinase for TGFb superfamily HSC, NSCLC 13, 26 members TGF-b RI # Abbreviations: ALL, acute lymphoblastic leukemia; AML, acute myelogenous leukemia; HSC, hematopoietic stem cells; NSCLC, non–small cell lung cancer. cancer development. The past several years have been marked negatively regulated in glioblastoma xenografts and in depleted by a surge of reports elucidating the frequency and mechanisms stem-like glioblastoma cells treated with the c-MET inhibitor of involvement of the Musashi proteins, and particularly MSI2, crizotinib (39). In colon cancer, both stimulation by the in multiple forms of human cancer. This review focuses on these NOTCH ligand DLL4 and expression of activated NOTCH3 new findings, including the potential for drugging MSI2 as a increased MSI1 levels, whereas silencing of NOTCH3 by short therapeutic target. For comprehensive summaries of Musashi hairpin RNA reduced MSI1 levels in cancer cells and tumor biology, the reader is directed to recent excellent articles, including xenografts (40). In lung-infiltrating breast cancer cells, upregu- refs. 14, 21, 22. lation of MSI1 was dependent on expression of tenascin C (TNC), an extracellular matrix component that protects stem Elevated Musashi Protein Expression cells (31). 0 Characterizes Solid and Liquid Tumors: Posttranscriptionally, the MSI1 3 -UTR is regulated by direct interaction with another important RBP, HuR, which positively Evidence for Prognostic Biomarker Status regulated Msi1 expression in glioblastoma; expression of both Since the first implication of MSI1 and MSI2 as potential proteins was correlated in primary glioblastoma tumor samples contributors to cancer pathology, elevated expression of these (41). The Msi1 30-UTR region is also potentially targeted by proteins has been identified in many types of solid tumors several tumor suppressor miRNAs in glioblastoma and neuro- (Table 1) arising from organ sites including the brain, breast, blastoma (miR-34a, -101, -128, -137, and -138; ref. 41). The pancreas, colon, lung, ovary, bladder, and others (17, 23–33). finding of regulation by the tumor-suppressive miR-137 was In addition, elevated expression of Musashi proteins has been independently made in colorectal cancers (42). found in CML, acute myelogenous leukemia (AML), and acute Finally, in insights from noncancer models that may be relevant lymphoblastic leukemia (ALL; refs. 11, 12, 34–36). For
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