Studies on the Terrestrial and Freshwater Algae of Aldabra Atoll
Total Page:16
File Type:pdf, Size:1020Kb
Durham E-Theses Studies on the terrestrial and freshwater algae of aldabra atoll Donaldson, Alan How to cite: Donaldson, Alan (1978) Studies on the terrestrial and freshwater algae of aldabra atoll, Durham theses, Durham University. Available at Durham E-Theses Online: http://etheses.dur.ac.uk/8442/ Use policy The full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-prot purposes provided that: • a full bibliographic reference is made to the original source • a link is made to the metadata record in Durham E-Theses • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders. Please consult the full Durham E-Theses policy for further details. Academic Support Oce, Durham University, University Oce, Old Elvet, Durham DH1 3HP e-mail: [email protected] Tel: +44 0191 334 6107 http://etheses.dur.ac.uk STUDIES ON THE TERRESTRIAL AND FRESHWATER ALGAE OF ALDABRA ATOLL by Alan Donaldson (B.Sc. Dunelm) A thesis submitted for the degree of Doctor of Philosophy in the University of Durham, England The copyright of this thesis rests with the author. No quotation from it should be published without his prior written consent and information derived from it should be acknowledged. Cr SCIENCE % -7 FEB 1979 SECTION /j This thesis, which is entirely the result of my own work, has not been accepted for any degree, and is not being submitted concurrently in candidature for any other degree. STUDIES ON THE TERRESTRIiiL.-AND FRESIWATER ALGAE OF ALllABM ATOLL by Alan Donaldson (B.Sc. Dunelm) ABSTMCT A study was made of the terrestrial and freshwater algae of Aldabra Atollj Indian Ocean. Field work was carried out between Septrember 19 72 and June 1973. Wide ranging excursions were made to various areas of the atoll in order to collect information on the species present together with physico-chemical and descriptive data from representative habitats. Data were collected in a standard manner and stored in a computable form using a modified version of a stream recording system devised in Durham, 1053 samples were collected during the field studyj of xchich 521 were complete with detailed taxonomic and environmental data. The latter are stored on computer file at Durham. The 521 samples constituted an Aldabran data bank and a range of computer programmes facilitated the rapid retrieval of information and the performing of statistical analyses. An example of one such statistical analysis is included in the present study. A taxonomic checklist is given of the 29Z species together V7ith descriptions of these species, comparisons with the descriptions given by other authors, the areas of Aldabra from which they v/ere recorded, notes on distribution on Aldabra, habitats, abundance and com.parisons with records of other vrorkers. Blue-green algae are the most abundant species present, predominating in both terrestrial and freshwater habitats. In terrestrial habitats they are often present almost to the exclusion of other species, though in freshwater habitats this predominance is less x«?ell-marked. It is probable that some species are restricted to certain regions or habitats: the reasons for such restriction are discussed. A study of one physiological process in biue-green algae is presented. The nitrogen-fixing potential of Tolypothrix byssoidea and several Hostoc species was investigated using the acetylene reduction assay technique. The study includes rates measured between 0930 and 1200 h, a 24 h time course study for Kostoc Cornm^'^A&. and laboratory studies on the onset of acetylene reduction after revetting. ACKNOVJLEDGMENTJ It is a pleasure to acknowledge the many people who have helped rae during the period of this work. I am especially indebted to Dr B. A. Whitton, my supervisor, for his original inoculation and further culture of ray interest in blue-gresn algae. • This work would not have been possible without the splendid support of the Royal Society and all those concerned with the conservation of Aldabra. In particular I should like to thank Mr L. U. Mole and Mr D. J. H, Griffin who ensured efficient transport of myself and my equipment to and from Aldabra. While on Aldabra the staff of the Royal Society Research Station gave invaluable assistance with travel on the atoll and in discussion. VJithout George Larue, Mazarin, Harry Charles, Andrew Quatre and Bernard Legae travel and collection of data on Aldabra would have been so much more difficult. Harry Vidot and Octavien ensured'that I was well fed. In Durham Mr J. W. Simon gave invaluable technical and laboratory assistance. Dr C. Sinclair and Dr J. W. Hargreaves provided helpful discussion in the development of laboratory • techniques. I wish to reserve special thanks to Mr B. M. Diaz v;ho spent many hours developing the computing techniques used and instructing me in their use. My thanks to Professor D. Boulter and the staff both academic and technical of the Botany Department, Durham for the many pleasant years spent there, and' to Mr Vv . B, Woodvjard and the staff of the Science Library. Finally I am extremely grateful to Mrs H. Winn for the skill and patience she has shown in typing this thesis. There are many other people who have assisted in the making of this thesis, and T am sure that they know that I am grateful to them all and will forgive their omission from this list. This work was carried out with a grant awarded to Dr B. A. Whitton from the Natural Environmental Research Council with the full support of the Council of the Royal Society Aldabra Research Committee, to both of which I am most grateful. 1 CONTENTS page List of Tables 5 List of Figures 8 1 INTRODUCTION 10 1.1 Algal vegetation of atolls and related 10 habitats 1.11 Coral islands and atolls 10 1.12 Other types of tropical island 13 1.2 Environmental factors 14 1.21 Subaerial and terrestrial 15 communities t.22 Freshwater communities 18 1.23 Algae and carbonate deposits 19 1.231 Chemistry of carbonate 20 1.232 Deposition of carbonate 23 1.233 Erosion of carbonate 24 1.3 Aldabra 25 1.31 Geography 25 1.32 Climate 26 1.33 Geomorphology 26 1.34 Flora and fauna 34 1.341 Flora 34 1.342 Fauna 35 1.35 Influence of man on Aldabra 37 1.4 Taxonomy of blue-green algae 3-8 1.5 Nitrogen fixation 42 1.6 Aims of project 44 FEB 1979 SECTION 1 page 2 MATERIALS AND METHODS 47 2.1 List of abbreviations and symbols 47 2.2 Collection, preservation and exam- 48 ination of specimens 2.21 Collection 48 2.22 Preservation 75 2.23 Examination 81 2.3 Physical and chemical techniques 83 2.31 Meteorological 83 2.32 Analysis of water 84 2.321 Conductivity 89 2.322 Optical density 89 2.323 Temperature 89 2.32 4 pH 90 2.325 Dissolved oxygen 90 2.326 Cations 91 2.327 Anions 91 2.33 Analysis of photopigments 92 2.331 Introduction 92 2.332 Extraction of chlorophyll a 98 2.333 Estimation of chlorophyll a 99 2.334 Uptake of moisture in three 101 types of Nostoc colony 2.335 Effect of drying and re- 101 wetting on the chlorophyll a content of Nostoc commune 2.34 Estimation of total nitrogen 102 2.35 Dry weight determination 103 3 page 2.36 Isolation and culture 103 2.361 Apparatus for the growth 103 of cultures 2.362 Measurement of physical 104 features 2.363 Cleaning of glassware 104 2.364 Sterilisation 105 2.365 Media 105 2.37 Nitrogen fixation 108 2.38 Statistics and computing 111 2.381 Parametric tests 111 2.382 Non-parametric tests 114 2.383 Recording, storage and 115 analysis of data 3 ENVIRONMENTAL DATA 128 3.1 Meteorological 128 3.2 Analysis of water 133 3.21 Conductivity 133 3.22 Optical Density 151 3.23 Temperature 151 3.24 pH 152 3.25 Dissolved oxygen 152 3.26 Cations 152 3.27 Anions 153 3.271 Chloride 153 3.272 Phosphate 153 3.273 Inorganic combined nitrogen 154 3.3 Algal cultures 156 page 4 SYSTEMATIC ACCOUNT 159 4.1 Species list 159 4.2 Description of species 160 4.3 Statistical Analysis 465 5 ACETYLENE REDUCTION STUDIES 478 6 DISCUSSION 486 6.1 Species composition ' 486 6.2 Environmental factors 489 6.3 Climate 498 6.4 Flora ,505 6.5 Fauna 506 6.6 Influence of man 508 6.7 Chemistry of pools 509 6.8 Nitrogen fixation 512 6.9 Future studies 516 SUMMARY ' , 518 APPENDIX 520 REFERENCES ' 5 29 5 List of Tables Page Chapter 2 2.1 Aldabran pools included in the present 66 survey. 2.2 Physiognomic and environmental categories 76 recorded for each sample. 2.3 Sampling programme for water chemistry. 87 2.4 The degradation of chlorophyll £ extracts 96 in methanol when stored under different conditions. 2.5 Effect of drying and rewetting on the 97 amount of chlorophyll a extracted from ' colonies of Nostoc commune. 2.6 Composition of media (mg 1 ^ of elements). 106 2.7 Composition of media (mg 1 ^ salt) 107 2.8 Simple variables for the Aldabra data set. 127 Chapter 3 3.1 Meteorological data collected at the 129 synoptic observation ^le Picard during the period of this survey. 3.2 Rainfall totals for periods October - June 131 inclusive 1967 - 1974. 3.3 Temperature of selected terrestrial sites 132 on Tie Picard.