234 3 Y XIE and others Lh and Fsh signaling in 234:3 233–246 Research zebrafish spermatogenesis The highly overlapping actions of Lh signaling and Fsh signaling on zebrafish spermatogenesis Yuxin Xie1, Lianhe Chu1, Yun Liu1, Kathy W Y Sham1, Jianzhen Li1,2 and Correspondence Christopher H K Cheng1 should be addressed 1School of Biomedical Sciences, The Chinese University of Hong Kong-Shandong University Joint Laboratory to J Li or C H K Cheng on Reproductive Genetics, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China Email 2College of Life Sciences, Northwest Normal University, Lanzhou, China
[email protected] or
[email protected] Abstract Gonadotropin signaling plays a pivotal role in the spermatogenesis of vertebrates, but Key Words exactly how gonadotropins regulate the process in non-mammalian species remains f Fsh elusive. Using a gene knockout approach in zebrafish, we have previously demonstrated f Lh the non-canonical action of gonadotropin signaling on spermatogenesis by analyzing f testis four single mutant lines (lhb, lhr, fshb and fshr) and three double mutant lines (lhb;fshb, f spermatogenesis lhr;fshr and fshb;lhr). In this study, we further investigated the actions of gonadotropins f zebrafish Endocrinology on the testis by establishing three other double-mutant zebrafish lines (lhb;lhr, fshb;fshr of and lhb;fshr). All lhb;lhr and fshb;fshr mutant males were fertile. Analysis on the gonadosomatic index and testicular histology in these lhb;lhr and fshb;fshr mutants Journal demonstrated that Lh signaling and Fsh signaling could functionally compensate each other in the testis.