Agilent Technologies, Inc

SOLICITATION: RFB 10850 I OPENING DATE: Thursday, April 22, 2021

PROJECT: LIQUID CHROMATOGRAPHY SYSTEM

BIDDER BASIS OF AWARD PREFERRED VENDOR ADJUSTED PRICE

Aligent $378,035.10 NIA

Water Technologies $314,644.43 NIA

Bid Officer: Bid Clerk:

This Notice oflntent to Award I Abstract ONLY indicates the APPARENT low bidder. Conditions that may displace an apparent low bidder include, but are not limited to: math errors, conditioning of bid, mistake in bid, failing pre-award Survey, and the bid being non responsive.

Bids Received Agilent Technologies, Inc. 800 227 9770 telephone LSCA Customer Sales and Service 302 993 5941 facsimile 2850 Centerville Road, MS 3J7 www.agilent.com Wilmington, DE 19808 Email: [email protected]

April 21, 2021

County of San Diego 5560 Overland Avenue, Suite 270 San Diego, CA 92123-1204 Attn: Lina Kim

Re: RFB - 10850

Dear Lina,

Agilent Technologies, Inc. (hereinafter, “Agilent”) is pleased to respond to the subject Request for Quote.

Agilent (NYSE: A) is a preeminent leader in the life sciences, diagnostics, and applied chemical markets worldwide. The company provides laboratories all over the world with exceptional instruments, services, consumables, applications and expertise, enabling its customers to gain the insights and knowledge that they seek. Agilent’s expertise and trusted collaboration provide its customers with the highest confidence in our solutions.

Agilent is bidding in accordance with Agilent’s Quotation # 3545728 (the “Quotations”) with 1290 Infinity II / G6230BA in lieu of the specified Water’s instrumentation in response to the attached Request for Quote RFB – 10850 the “RFQ”), and is in full compliance with the requirements of the RFQ and any amendments thereto.

Agilent Exception to Specifications: Section B, subsection 7 the dimensions are mentioned. Agilent’s system is larger (135 cm Height).

If you have questions regarding Agilent’s products listed in the Quotation, please do not hesitate to contact the undersigned at 302-636-1575 or by e-mail at [email protected]. Thank you in advance.

Sincerely, AGILENT TECHNOLOGIES, INC.

Elaine Jones Bids Response Specialist

JOHN M. PELLEGRINO DEPARTMENT OF PURCHASING AND CONTRACTING ALLEN R. HUNSBERGER DIRECTOR ASSISTANT DIRECTOR 5560 OVERLAND AVENUE, SUITE 270, SAN DIEGO, CALIFORNIA 92123-1204 Phone (858) 505-6367 Fax (858) 715-6452

April 9, 2021

COUNTY OF SAN DIEGO - REQUEST FOR BIDS (RFB) 10850 SHERIFF’S DEPARTMENT - CRIME LAB LIQUID CHROMATOGRAPHY SYSTEM AND MAINTENANCE SERVICES

The County of San Diego is seeking bids from firms interested in providing Liquid Chromatography System and Maintenance Services (“Offerors”).

The Basis of Award will be the total bid price. Offeror must respond to all bid items to be considered for award.

The resulting contract will be for initial (base) period from the date of the last signature through June 30, 2022 with four (4) one-year options.

The Contracting Officer for this solicitation is Lina Kim, Procurement Specialist ([email protected]).

This RFB package includes: This Cover Letter to the RFB RFB Instructions and Rules Submittal Items Bid Cover Page (PC 600 Form) Representations and Certifications Form Nondisclosure Indemnification Agreement DVB Requirements and Forms Agreement, which includes the following: Exhibit A - Statement of Work Exhibit B - Insurance Requirements Exhibit C - Payment Schedule

SCHEDULE

Below is the County’s timeline for this RFB, and is subject to change at any time. The County will issue an addendum for changes to the Questions due date, or Bid due date. The actual timing and sequence of events resulting from this RFB shall ultimately be determined by the County.

Event Description Date and Time (San Diego Time) Questions due April 15, 2021 prior to 5:00 p.m. Bids due April 22, 2021 prior to 11:00 a.m. COUNTY OF SAN DIEGO – REQUEST FOR BIDS (RFB) 10850 SHERIFF’S DEPARTMENT - CRIME LAB LIQUID CHROMATOGRAPHY SYSTEM AND MAINTENANCE SERVICES

BID OPENING The County has temporarily changed procurement processes due to the social distancing and other requirements put in place in response to the COVID-19 pandemic. The County will conduct a public bid opening using the WebEx platform instead of in-person attendance. • Virtual bid opening link: https://sdcountyca.webex.com/sdcountyca/j.php?MTID=m40ab756a8c450531c56ea7de20e5c891 • Password (if requested): cosd_bidopening • You may also join by phone by calling (1‐470‐238‐5742), access code 177 120 2660

A bid abstract and the bids received will be posted on BuyNet after the bid opening.

BID SUBMITTAL

The County has temporarily changed procurement processes due to the social distancing and other requirements put in place in response to the COVID-19 pandemic. Unless otherwise modified by the County’s COVID-19 Updates for County Contracts and Contractors, found at https://www.sandiegocounty.gov/content/sdc/purchasing/COVID-19_Updates.html, the following procedures shall apply to this RFB:

 This RFB is posted to the BuyNet site with a solicitation type of RFP, to allow the submission of electronic bids through BuyNet.  Offeror must submit a complete original bid in accordance with the format provided in this solicitation to the County of San Diego, Department of Purchasing and Contracting through electronic upload to BuyNet by the Date and Time listed in Schedule.  If Offeror is unable to submit via BuyNet for any reason, Offeror may submit via electronic files on CD/DVD or a printed bid at the Purchasing and Contracting front desk. Please use this method only if unable to submit through BuyNet. Bids will be accepted at the front desk by mail or courier service only. The County will not accept personal delivery by Offerors or Offeror’s representatives. When submitting via BuyNet, allow extra time to submit by an alternate method in the event of technical difficulties.  When submitting electronic files, Offeror may optionally submit in original format (e.g. Microsoft Word) with clear pictures (.jpg, .jpeg, .gif, or ".png") of signature pages containing original signatures. Electronic or digital signatures will not be accepted. Please use this method only if unable to submit in PDF form. The County's decision about the timeliness or responsiveness of any submitted document shall be final, and the County has the discretion to waive or not waive any defect or nonconformance.

Additional COVID-19 procurement information is available at: https://www.sandiegocounty.gov/content/sdc/purchasing/COVID-19_Updates.html

QUESTIONS

Questions regarding this RFB shall be submitted in writing to the Contracting Officer by the Date and Time listed in Schedule, in accordance with Section 8 (Duty to Inquire, Questions, and Explanation to Offerors). Oral explanations or responses to Questions will not be binding upon the County.

Concerns or comments regarding this solicitation should be directed to the Contracting Officer.

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RFB INSTRUCTIONS AND RULES

1. RFB PROCESS 1.1. RFBs shall normally be made available on the County of San Diego’s BuyNet Website at https://buynet.sdcounty.ca.gov/. If you are unable to download this document, you may request a hard copy from Purchasing and Contracting’s front desk or contact clerical support at (858) 505-6367. 1.2. The County reserves the right to host pre-bid conference(s). If scheduled, the date, time, and location for the pre- bid conference(s) will be as set forth in this RFB or as otherwise provided on BuyNet. 1.3. Offerors should submit all Questions by due date and time specified in the cover letter to this RFB. Questions received after the due date may or may not be addressed. 1.4. Bids received timely will be opened publicly following the due date and time. 1.4.1. Bids received and the abstract of responses will be posted to BuyNet after the bid opening. 1.5. The posted bid opening abstract of responses serves as Notice of Intent to Award (“NOI”) to the apparent low bidder(s). 1.6. The County will evaluate bids received for conformance to the requirements of this RFB through a pre-award survey process. As a result of the pre-award survey, County may determine that a bid is non-responsive to the County’s bid requirements or was submitted by a non-responsible bidder, as described in Section 3 below. 1.7. In the event the County finds an apparent low bid to be non-responsive or to have been submitted by a non- responsible bidder pursuant to Section 3 below, the County will post a revised NOI. The County may rescind or modify the NOI at any time. 1.8. If a contract is awarded, the County will award to the lowest responsive, responsible bidder(s) as determined by the County’s evaluation (“Successful Bidder”). Bids are subject to acceptance at any time within 120 days after bid opening. This period may be extended by mutual agreement of County and Offeror. 1.9. Offeror’s submittal of this bid constitutes an offer that, upon County’s acceptance (which may be of some or all of the bid items or lots in accordance with the Basis of Award) forms a binding contract that incorporates the Agreement, among all other terms of this RFB. Without limiting the foregoing, Offeror agrees to execute and deliver a revised contract document reflecting the final contract award and such additional documents and instruments as may be reasonably required in order to carry out the purposes of this RFB and the intentions of the parties. 1.10. In the event that an Offeror is involved in a merger, acquisition, or other change in control, the County reserves the right to award a contract to a resulting entity. 1.11. The County may, at any time, restart evaluations or correct any deficiencies in the procurement process or evaluation. 1.12. The timing and sequence of events resulting from this RFB shall ultimately be determined by the County.

2. INSTRUCTIONS FOR SUBMITTING A BID 2.1. It is the Offeror’s responsibility to submit a bid based on the most current RFB, addenda thereto, responses to Questions, any diligence material made available by the County, and any other information posted on BuyNet. Offerors must consistently check BuyNet for information and are responsible to comply with anything posted on BuyNet relating to this RFB. The County has no obligation to contact Offerors directly with any such RFB related information. 2.2. Offerors shall submit one original bid prior to the date and time specified and through the means specified in the cover letter to this RFB. 2.2.1. For bids submitted electronically through BuyNet, the time on the BuyNet server shall be considered the official time for purposes of determining timely submittal. 2.2.2. For bids submitted at the Purchasing and Contracting front desk, the time stamp at the front desk shall be considered official time for purposes of determining timely submittal. 2.2.3. In the event of multiple submittals of an original bid by an Offeror, the County will only consider the most recent submittal submitted by the due date and time. The County will not review or consider previous submittals, whether submitted at the front desk or through BuyNet. Offerors are strongly encouraged to submit only one original bid and withdraw or replace any previous submittals in the event they choose to

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update their bid. Bids may be found non-conforming if the County is unable to determine which is the most recent, timely submittal. 2.3. Bids must be submitted using the County forms provided in this RFB, accepting the County’s statement of work, terms and conditions, and other requirements without condition. Bids containing modifications, conflicting information, or competing terms may be found non-responsive. Bidders are advised to submit only the requested information. 2.3.1. All bids shall be signed by an authorized officer or employee of the Offeror. The name, title, mailing address, email address, and phone number of the authorized officer or employee shall be included. Scanned images of pages containing original signatures are acceptable for submission of an electronic file. Electronic or digital signatures will not be accepted. 2.4. The bid must be submitted in compliance with the following format, unless otherwise specified in this RFB: 2.4.1. Submit electronic bids as files in .pdf format. Separate each exhibit into one or more files. Clearly name files as to the exhibit and order. Pages requiring signatures must be scanned from an original signature. Other pages may be scanned or converted to .pdf from other file formats. Converted and searchable formats are preferred. 2.4.2. All information submitted with your bid shall be formatted to print on 8-1/2” x 11” page size with no less than ½” margins and eleven (11) point font. 2.4.3. All information submitted with your bid shall be formatted as black ink on white background/paper with no shading, or otherwise suitable for black and white reproduction. 2.4.4. If submitting your bid at the Purchasing and Contracting front desk, it must be submitted in a sealed envelope with “RFB” and the RFB number prominently marked on the outside of the envelope. 2.5. Your bid must be organized in accordance with this RFB. The bid shall conform to the following organization: 2.5.1. A completed and signed PC 600 Form shall be submitted as the cover of Offeror’s bid. 2.5.2. Completed and signed Representations and Certifications form. 2.5.3. Completed Exhibit C Payment Schedule. 2.5.4. Completed and signed Nondisclosure Indemnification Agreement (if applicable). 2.5.5. Required supplemental information per the Submittal Items. 2.5.6. Confidential/Proprietary exhibit (if applicable). 2.6. Confidential/Proprietary Information 2.6.1. No confidential/proprietary information, including PI (Personal Information), PII (Personally Identifiable Information) or PHI (Protected Health Information), is to be included in the supplemental information. Responses that include the confidential/proprietary information shall refer to the response contained within the Confidential/Proprietary exhibit (for example: If Submittal Item #1 requires staff Social Security Numbers, the response to Submittal Item #1 shall reflect “see response #1 contained within Confidential/Proprietary exhibit). 2.6.2. Submit a separate Confidential/Proprietary exhibit (if applicable). The County is a public agency subject to the disclosure requirements of the Public Records Act, California Government Code Section 6250 and following. The County of San Diego intends to publish bids received and resulting contracts, to its public web site. If confidential/proprietary information is contained within the submission: 2.6.2.1. It must be submitted in a separate clearly labeled exhibit with all pages marked as “CONFIDENTIAL/PROPRIETARY EXHIBIT”. 2.6.2.2. Offeror must provide a signed Nondisclosure Indemnification Agreement. 2.6.2.3. It shall only contain supplemental information. In accordance with the California Public Records Act, the County will not treat pricing or any bid forms as confidential. 2.7. Bid Pricing 2.7.1. Submit bid pricing on the included Exhibit C Payment Schedule. Bid using only the units of measure stated in the Payment Schedule and price each bid item clearly and separately (do not aggregate bid items nor provide more than one price or a range of prices per item). 2.7.1.1. Pricing shall not exceed two (2) digits to the right of decimal point (hundredths). If unit pricing exceeds two (2) digits to the right of the decimal point, any digit(s) beyond two (2) will be dropped and will not recognized by the County for the purposes of bid evaluation, contract award, or invoicing. The County will only use the first two digits after the decimal points without

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rounding up or down. If the County calculates a “new” unit price, said unit price will be in favor of the County and may be below the Contractor’s original bid price. The “new” unit price shall prevail over Contractor’s original bid price and shall be used in evaluation of bids, award, and invoicing. 2.7.2. All prices and notations must be clearly legible and in ink or typewritten. No erasures permitted. Mistakes may be crossed out and corrected and must be initialed in ink by person signing the bid. 2.7.3. Submitted bid prices must be balanced (reflecting the true relative costs of the bid items), and not shift costs from one portion of the work to another portion of the work so as to place doubt on whether the bid will result in the lowest overall cost to the County or be tantamount to allowing an advance payment. 2.7.4. Unless otherwise specified, all prices shall be F.O.B. destination. 2.7.5. Prices shall include all freight and/or delivery charges, unless freight or delivery charges are separately itemized or otherwise allowed for in the Payment Schedule. 2.7.6. Unless otherwise specified, prices bid herein should NOT include California sales/use tax or Federal excise tax. The County generally is required to pay California sales/use tax, and it should be shown as a separate item on invoices. The County is exempt from payment of Federal excise tax. It must NOT be included in invoices. 2.7.7. Do not include prompt payment discounts into your bid prices. Prompt payment discounts will not be considered in evaluation of bids to determine low bidder. 2.7.8. Prices shall be fixed during the performance of the Agreement and not subject to adjustment except as explicitly set forth in the Agreement. Bidder accepts the risk of any changes to bidder’s costs during the term of the Agreement. 2.7.9. Estimated quantities may be included in the Payment Schedule, and are provided solely for evaluation of bids. They represent approximate anticipated use based on historical consumption or other estimates. If the County’s actual requirements do not result in orders in the quantities described as estimated in the Payment Schedule, that fact shall not entitle the successful bidder(s) to price adjustment or other relief. 2.7.10. RESERVED 2.8. Brand Name or Equal 2.8.1. If items in this RFB have been identified as brand name “or equal,” such identification establishes a quality standard and is not intended to limit competition. Other brands will be considered for award if their products are determined by the County to be of equal or greater quality and to meet the County’s requirements. 2.8.1.1. Bidder shall clearly identify the brand name of items that are not of the brand specified by the County. Bids shall be considered as offering the specified brand unless otherwise indicated in the bid. 2.8.2. The County’s evaluation as to equality of a product offered by a different brand shall be based upon the information furnished in the bid or pre-approval request, as well as other information known to the County. It is the bidder’s responsibility to provide sufficient information for evaluation. 2.8.2.1. If bidder proposes to modify a product so as to make it conform to the requirements, bidder shall include in the pre-approval request a clear description of such modifications. 2.8.2.2. Modifications proposed after bid submission will not be considered. 2.8.3. If bidder proposes to furnish an equal product, bidder must submit a request for pre-approval, including sufficient information for the County to evaluate the proposed equal item. The County’s evaluation as to equality of the product offered shall be based upon the information furnished in the bid as well as other information known to the County. It is the bidder’s responsibility to provide sufficient information for evaluation. Bidder must submit requests for pre-approval at least 5 days before the bid due date and time. If the County accepts the proposed equal item, the County will issue an addendum stating its acceptance. 2.9. RESERVED 2.10. All bids become the property of the County. An Offeror may request the return of its bid upon withdrawal as specified in Paragraph 6, which return County may grant or deny in its sole discretion. 2.11. County has the right to withhold all information regarding this procurement (other than information disclosed through public bid opening) until after contract award, including, but not limited to, the County pre-award survey

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and results. Information releasable after award is subject to the disclosure requirements of the Public Records Act, California Government Code Section 6250 and following. 2.12. RESERVED

3. EVALUATION AND SELECTION 3.1. Award will be made to the lowest priced responsive bid(s) from a responsible bidder(s). The determination of lowest price shall include the application of the local preference, when applicable. 3.1.1. Award may be based on total bid amount, distinct lots, or individual items (“Basis of Award”). 3.1.2. When determining lowest price for award, the County will not consider prompt payment discounts. 3.1.3. In the event of a conflict between unit price bid and bidder's extended price, the unit price will prevail unless the conflict is a result of a Mistake in Bid. In that event, the bid will be rejected as non-responsive. 3.1.4. In the event of a tie bid between responsible bidders, award will be by random selection through a process determined by County and observable by the bidders involved in the tie. Should one or more of the bidders involved in the tie be a Preferred Vendor, award will be made as described in 3.3 below. 3.2. The County reserves the right to perform a pre-award survey to determine whether a bidder is responsible (possessing the attributes of trustworthiness, quality, fitness, capacity, and experience to satisfactorily perform the contract) and whether a bid is responsive (conforming with material bid requirements). 3.2.1. As part of the pre-award survey, The County reserves the right to request clarification of and/or additional information (“Clarification”) from Offerors. 3.2.2. The Contracting Officer will determine the appropriate means of Clarification. The County is not obligated to seek Clarification, so Offerors are advised to submit complete and accurate information in the bid. 3.2.3. The request for Clarification is not an opportunity for a bidder to modify their bid, but only to provide additional information requested by the County to support the pre-award survey. 3.2.4. The County may request that samples be provided as part of the bid process, including with the bid submission, when evaluating proposed equal items, or during the pre-award survey. Samples must be furnished free of expense to the County, prominently marked with the RFB number, and delivered to the location specified by the County. Upon request and where feasible, samples will be returned to bidder at bidder’s expense and risk when County review is complete. 3.2.5. Failure to timely provide requested information or samples may result in a finding of non-responsiveness or non-responsibility based on the information available to the County. 3.2.6. The County may consider information known to the County in addition to the information provided in response to the RFB. 3.2.7. The County has the discretion to treat each lot or line item separately for purposes of evaluation, in accordance with the Basis of Award. 3.2.8. The County has the discretion, but not the obligation, to waive any minor irregularities or deviations from the requirements of the RFB. The County reserves the right to waive a variation in specification if County determines such variation does not materially change performance. 3.2.9. The County’s determination of responsibility or responsiveness shall be conclusive. 3.3. Local preference: In accordance with Section 405 of the San Diego County Code of Administrative Ordinances, five percent (5%) or fifty thousand dollars ($50,000), whichever is less (“Price Adjustment”), shall be subtracted from the bid price of any Preferred Vendor (a Local Business that is also a Veteran Owned Business, Disabled Veteran Business, or Small Business) being evaluated for award (“Adjusted Price”). A Preferred Vendor’s Adjusted Price shall be the basis for award to the lowest responsible bidder. In the event that one Preferred Vendor is involved in a tie for the lowest responsible bidder, the Preferred Vendor shall be awarded the contract. In the event that more than one Preferred Vendor is involved in a tie for the lowest responsible bidder, the County shall randomly select among those Preferred Vendors for contract award.

If a contract is awarded to a Preferred Vendor, it shall be awarded at the unadjusted bid price. For example, a Preferred Vendor submitting a bid for $100,000 would be evaluated as if the bid price was $95,000. If based on the Adjusted Price, the Preferred Vendor is the lowest responsible bidder, the Preferred Vendor would be awarded the contract at the unadjusted price of $100,000.

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4. DETERMINATION OF ECONOMY AND EFFICIENCY Pursuant to County Charter Sections 703.10 and 916, award of a contract resulting from this RFB may be subject to the County’s requirement to determine that the services can be provided more economically and efficiently by an independent contractor than by persons employed in the Classified Service.

5. COUNTY COMMITMENT 5.1. This RFB does not commit the County to award, nor does it commit the County to pay any cost incurred in the submission of the bid, or in making necessary studies or designs for the preparation thereof, nor procure or contract for services or supplies. Further, no reimbursable cost may be incurred in anticipation of a contract award. 5.2. The County reserves the right to accept or reject any or all bids received as a result of this RFB or to separately procure the same or similar goods or services. 5.3. The County reserves the right to terminate this RFB in part or in its entirety at any time prior to contract execution. 5.4. No prior, current, or post award communication with any officer, agent, or employee of the County shall affect or modify any terms or obligations of this RFB except as explicitly provided for in this RFB.

6. LATE, MODIFIED, OR WITHDRAWN BID 6.1. A bid that is received, but that is not received at the office or by the electronic means designated in the solicitation by the exact time specified for receipt will not be considered unless: it is received before award is made; the County determines that it is in its best interest to accept the bid; and 6.1.1. It was sent by mail or personal delivery, and it is determined by the County that the late receipt was due solely to mishandling by the County after receipt by the County; or 6.1.2. No responsive bid was submitted. 6.2. Bids may not be modified after the due date and time. Bids may not be withdrawn after the due date and time except in the event of a Mistake in Bid, provided that the Offeror notifies the County of the Mistake in Bid, specifying in detail the mistake and how the mistake occurred, within five (5) business days. 6.3. A mistake in bid (“Mistake in Bid”) is defined as a mistake that (1) made the bid materially different than the bidder intended it to be, and (2) was made in filling out the bid and not due to error in judgment or to carelessness in inspecting the site of the work, or in reading the requirements.

7. DILIGENCE 7.1. County, its agents, advisors, and representatives make no representation or warranty, express or implied, as to the accuracy or completeness of any provided diligence material. Without limiting the generality of the foregoing, the diligence material may include certain statements, estimates, and projections provided by or with respect to the County. Such statements, estimates, and projections reflect various assumptions made by the County, which may or may not prove to be correct. No representations are made by the County as to the accuracy of such statements, estimates, projections, or assumptions. 7.2. Offerors are expected to perform reasonable due diligence with regard to the work required. The Selected Bidder(s) shall not be entitled to any price adjustment or other relief based upon information that was discovered or should have been discovered through due diligence. Such due diligence shall include but not be limited to, information that can be obtained during a site visit, if applicable (including verification of measurements, conditions, and other attributes of the site), or independently to the extent that information about the work required or the site is otherwise available for inspection or review.

8. DUTY TO INQUIRE, QUESTIONS, AND EXPLANATION TO OFFERORS 8.1. Offerors’ Inquiries and County Responses – All communications from the Offeror (including its employees, agents, and representatives) to the County or its officers and employees (including consultants working on or assisting with this procurement), related to this RFB or the Offeror’s bid, must be directed in writing exclusively to the Contracting Officer, unless otherwise authorized in writing by the Contracting Officer. Any improper contact may, at County’s sole discretion, cause the Offeror to be removed from consideration for contract award. 8.2. Should an Offeror find discrepancies in or omissions from, or be in doubt as to the meaning of, the RFB or related documents, Offeror shall have a duty to at once notify the County. Such notifications, or other requests for explanation regarding the RFB or related documents, shall be directed to the Contracting Officer in writing as a RFB 10850 Liquid Chromatography System and Maintenance Services Page 7 of 31 COUNTY OF SAN DIEGO – REQUEST FOR BIDS (RFB) 10850 SHERIFF’S DEPARTMENT - CRIME LAB LIQUID CHROMATOGRAPHY SYSTEM AND MAINTENANCE SERVICES

question (“Question”). Offeror is responsible for ensuring that Questions are received by the County. The County may choose not to respond to Questions received after the date stated in the Cover Letter. Offerors should not communicate with or attempt to contact any other County personnel about this solicitation, except as otherwise allowed for in this RFB

9. PROTEST PROCEDURE County Board of Supervisors Policy A-97 sets forth the procedures for filing and resolution of protests related to this RFB. Board Policy A-97 is available from the Clerk of the Board, 1600 Pacific Highway, San Diego, CA 92101, or on the County’s Web site at http://www.SanDiegoCounty.gov/ under the Clerk of the Board’s page.

All protests must be filed in accordance with Board Policy A-97, which requires among other things that a protest must be filed timely, be submitted in writing to the Contracting Officer identified in the solicitation package, and be based on a protestable action and established grounds for protest.

10. RESERVED

11. OFFEROR COMMUNICATIONS 11.1. If Offeror issues any public announcement or otherwise engages in communication that, in the County’s sole determination, compromises the integrity of this RFB process or attempts to restrain competition, Offeror may be removed from consideration for award. 11.2. Audio and/or video recording of pre-bid conferences, presentations, discussions, negotiations, debriefings, or other communications with the County regarding this RFB are prohibited, unless specifically authorized in writing by the Contracting Officer.

12. CLAIMS AGAINST THE COUNTY Neither Offeror nor any of its representatives shall have any claims whatsoever against the County or any of its respective officials, agents, or employees arising out of or relating to this RFB or these procedures (other than those arising under a definitive contract with Offeror in accordance with the terms thereof).

13. SOLICITING EMPLOYEES Until contract award, Offerors shall not, directly or indirectly, solicit any employee of the County to leave the County’s employ in order to accept employment with the Offeror, its affiliates, actual or prospective contractors, or any person acting in concert with the Offeror, without prior written approval of the County’s Contracting Officer. This paragraph does not prevent the employment by an Offeror of a County employee who has initiated contact with the Offeror.

14. PROHIBITED CONTRACTS 14.1. In accordance with Section 67 of the San Diego County Administrative Code, the County shall not contract with, and shall reject any bid or proposal submitted by the person or entities specified below, unless the Board of Supervisors finds that special circumstances exist which justify the approval of such contract: (a) Persons employed by the County or of public agencies for which the Board of Supervisors is the governing body; (b) Profit making firms or businesses in which employees described in subsection (a) serve as officers, principals, partners or major shareholders; (c) Persons who, within the immediately preceding twelve (12) months, came within the provisions of subsection (a), and who (1) were employed in positions of substantial responsibility in the area of service to be performed by the contract, or (2) participated in any way in developing the contract or its service specifications; and (d) Profit making firms or businesses in which the former employees described in subsection (c) serve as officers, principals, partners or major shareholders. 14.2. Offeror certifies it is not a person or entity specified above and that it will promptly notify the County in the event it becomes a person or entity specified above.

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15. CALIFORNIA REVENUE AND TAXATION CODE SECTION 18662 In compliance with California Revenue and Taxation code section 18662, if Offeror is a non-resident of California (out- of-state invoices) that receives California source income and has not completed FTB Form 590, there may be a backup withholding on all payments. Fifteen (15) business days prior to the first payment, new suppliers or suppliers with expired forms or forms with incorrect information must submit new forms to the County (forms are available from the Franchise Tax Board website listed below).

Under certain circumstances, Offerors may be eligible for reduced or waived nonresident withholding. If Offeror has already received a waiver or a reduced withholding response from the State of California and the response is still valid, Offeror should submit the response to the County in lieu of the forms. Failure to submit the required forms will result in withholding of payments. Offerors should to the Franchise Tax Board websites (listed below) for tax forms and information on non-resident withholding, including waivers or reductions. The County will not give Offerors any tax advice. It is recommended that Offerors speak with their tax advisers and/or the State of California for guidance. Franchise Tax Board Websites:

https://www.ftb.ca.gov https://www.ftb.ca.gov/forms/Search/Home/FormRequest/1619 https://www.ftb.ca.gov/forms/search/

If selected for award, the Offeror is to submit forms to the Auditor & Controller via fax at (858) 694-2060 or mail originals to: County of San Diego, 5530 Overland Avenue, Suite 410, San Diego, CA 92123. The P.O. Number or Contract Number (if available) and “California Revenue and Taxation Code Section 18662” must appear on fax cover sheet and/or the outside of the mailing envelope.

16. W-9 FORM If selected for award, the Offeror must complete and submit a W-9 form if a current form is not on file with the County.

(Remainder of this page left blank)

RFB 10850 Liquid Chromatography System and Maintenance Services Page 9 of 31 COUNTY OF SAN DIEGO – REQUEST FOR BIDS (RFB) 10850 SHERIFF’S DEPARTMENT - CRIME LAB LIQUID CHROMATOGRAPHY SYSTEM AND MAINTENANCE SERVICES

SUBMITTAL ITEMS

Offeror shall provide the requested supplemental information in response to the following Submittal Items in support of the County’s pre-award survey evaluation of responsiveness and responsibility. The failure to respond to all of the following Submittal Items may be grounds for the County to find the bid non-responsive.

Do not include any information in your response to these Submittal Items that modifies or conflicts with the requirements of this bid, including the Statement of Work and Agreement.

If confidential/proprietary information is required to respond to these Submittal Items, include it in a separate CONFIDENTIAL/PROPRIETARY exhibit in accordance with section 2.6 of the RFB Instructions and Rules.

For this solicitation: Additional Supporting information is not required for this RFB. Do not submit any additional information other than the required bid forms.

RFB 10850 Liquid Chromatography System and Maintenance Services Page 10 of 31

COUNTY OF SAN DIEGO – REQUEST FOR BIDS (RFB) 10850 SHERIFF’S DEPARTMENT - CRIME LAB LIQUID CHROMATOGRAPHY SYSTEM AND MAINTENANCE SERVICES

DVB REQUIREMENTS AND FORMS

The County, as a matter of policy, encourages the participation of Disabled Veterans Businesses (DVB) through DVB Subcontractor Participation goals. County of San Diego, Board of Supervisors Policy B-39a Veteran Owned Business (VOB) and Disabled Veterans Business Enterprise (DVBE) Program is found at http://www.sdcounty.ca.gov/cob/policy/index.html#. The County DVB program recognizes the State of California DVBE certification, which may be found at http://www.dgs.ca.gov/PD and the federal SDVOSB certification, which may be found at https://www.va.gov/osdbu/verification/ .

For this solicitation:

DVB Subcontractor Participation Requirements are not applicable. Bidder/Offeror (Offeror) does not need to submit DVB documentation with its submittal.

RFB 10850 Liquid Chromatography System and Maintenance Services Page 14 of 31 COUNTY OF SAN DIEGO – REQUEST FOR BIDS (RFB) 10850 SHERIFF’S DEPARTMENT - CRIME LAB LIQUID CHROMATOGRAPHY SYSTEM AND MAINTENANCE SERVICES AGREEMENT

RFB 10850

LIQUID CHROMATOGRAPHY SYSTEM AND MAINTENANCE SERVICES

AGREEMENT

INCLUDES: Exhibit A – Statement of Work Exhibit B – Insurance Requirements Exhibit C – Payment Schedule

RFB 10850 Liquid Chromatography System and Maintenance Services Page 15 of 31 COUNTY OF SAN DIEGO – REQUEST FOR BIDS (RFB) 10850 SHERIFF’S DEPARTMENT - CRIME LAB LIQUID CHROMATOGRAPHY SYSTEM AND MAINTENANCE SERVICES AGREEMENT

COUNTY CONTRACT NUMBER ______AGREEMENT WITH ______FOR LIQUID CHROMATOGRAPHY SYSTEM AND MAINTENANCE SERVICES

This agreement (“Agreement”) is made and entered into effective as of the date of the last signature on the signature page by and between the County of San Diego, a political subdivision of the State of California (“County”) and ______(“Contractor”). For valuable consideration, the receipt and sufficiency of which are hereby acknowledged, the parties agree as follows:

1. Agreement. The Agreement shall consist of this document, Exhibit A Statement of Work, Exhibit A-1 RFB 10850 including all addenda and attachments (incorporated herein by reference), Exhibit B Insurance Requirements, and Exhibit C Payment Schedule. In the event of a conflict between any provisions of this Agreement, the following order of precedence shall govern: First (1st) this document; Second (2nd) Exhibit B; Third (3rd) Exhibit A; Fourth (4th) Exhibit C; and fifth (5th) Exhibit A-1.

2. Term. The initial term of this Agreement shall begin on the date of the last signature below and end on June 30, 2022 (“Initial Term”).

Option to Extend. The County shall have the option to extend the term of this Agreement for four (4) increments of one (1) year each for a total of four (4) years beyond the expiration of the Initial Term, not to exceed June 30, 2026, pursuant to Exhibit C Payment Schedule or other applicable pricing provisions of this Agreement. Unless County notifies Contractor in writing not less than thirty (30) days prior to the expiration date that the County does not intend to extend the Agreement, the Agreement will be automatically extended for the next option period.

3. Standard of Performance. Contractor shall, in good and workmanlike manner and in accordance with the highest professional standards, at its own cost and expense, furnish all of the labor, technical, administrative, professional and all other personnel, all supplies and materials, equipment, printing, transportation, training, facilities, and all other means whatsoever, except as herein otherwise expressly specified to be furnished by County, necessary or proper to perform and complete the work and provide the services required of Contractor by this Agreement. To the extent not in conflict with Exhibits A and A-1, Contractor shall perform all work under this Agreement in strict conformance to its bid, included herein by this reference, unless modified in accordance with this Agreement.

4. Payment. Pursuant to Exhibit C or other applicable pricing provisions of this Agreement, County agrees to pay a sum not to exceed $______for the initial term of this Agreement and a sum not to exceed $______for each of the # one- year option periods, for a maximum Agreement amount of $______in accordance with exhibit C Pricing schedule.

5. Sales and Use Tax. On invoices, Contractor shall show any sales or use tax as separate items, giving permit number authorizing collection of use tax. Contractor shall deduct any cash discount before computing sales or use Tax.

6. Notices. Notice to either party shall be in writing and personally delivered; sent by certified mail, postage prepaid, return receipt requested; or emailed to the County’s or Contractor’s representative designated below (or such party’s authorized representative). Any such notice shall be deemed received by the party (or such party’s authorized representative) on the earliest of the date of personal delivery, three (3) business days after deposit in the U.S. Mail, or upon sending of an email from which an acknowledgement of receipt has been received other than an out of office, unavailable, or undeliverable reply. RFB 10850 Liquid Chromatography System and Maintenance Services Page 16 of 31 COUNTY OF SAN DIEGO – REQUEST FOR BIDS (RFB) 10850 SHERIFF’S DEPARTMENT - CRIME LAB LIQUID CHROMATOGRAPHY SYSTEM AND MAINTENANCE SERVICES AGREEMENT

FOR COUNTY: FOR CONTRACTOR:

#Name and Title #Name and Title #Address #Address #Address #Address #Phone, FAX and email #Phone, FAX and email

Above contact is designated as the Contracting Officer’s Representative for this Agreement (“COR”).

7. Compliance with Laws. Contractor shall at all times perform its obligations hereunder in compliance with all applicable federal, State, and local laws, rules, and regulations, current and hereinafter enacted, including facility and professional licensing and/or certification laws, and keep in effect any and all licenses, permits, notices and certificates as are required. Contractor shall further comply with all laws applicable to wages and hours of employment, occupational safety, and to fire safety, health and sanitation.

8. County Policies and Requirements. Without limiting Section 7 above, Contractor shall specifically comply with the following, as applicable:

a. Board of Supervisors Policy A-79, Interlocking Directorates b. Board of Supervisors Policy A-120, Zero Tolerance for Fraudulent Conduct in County Services c. Board of Supervisors Policy B-67, Environmentally Preferable Procurement d. Board of Supervisors Policy C-25, County of San Diego Drug and Alcohol Use Policy e. Section 32.801, et seq. of the San Diego County Code of Regulatory Ordinances, Prohibitions of AIDS Discrimination f. Article IIIk (Section 84, et seq.) of the San Diego County Administrative Code, Affirmative Action Program for Vendors

9. DVB Participation. If this Agreement resulted from a solicitation containing Disabled Veteran Business (“DVB”) requirements and forms, such requirements and Contractor’s submitted forms are incorporated herein by reference to the extent not included as an Exhibit to this Agreement. Contractor shall make all commercially reasonable efforts to comply with all such DVB requirements, including meeting the DVB Percent of Utilization on Contractor’s DVB Subcontractor Participation Plan. Contractor shall maintain a rate of DVB utilization throughout the term of this Agreement that is reasonably in alignment with the progress of the Agreement (e.g. term, utilization, deliverables). Contractor shall provide to County, upon request, documentation sufficient to verify Contractor’s compliance with such requirements.

If in County’s determination, Contractor is not in compliance with all DVB requirements, County may take corrective action, which may include (i) requiring Contractor to submit a corrective action plan acceptable to County detailing actions the Contractor will take to fulfill its DVB requirements and/or (ii) withholding of payments to Contractor equivalent to the amount of DVB underutilization. Such corrective actions shall be in addition to any other remedies the County may have under this Agreement or at law or equity.

10. Preferred Vendor. If this Agreement resulted from a solicitation where Contractor claimed Preferred Vendor status in its response per Section 405 of the San Diego County Administrative Code, Contractor shall perform a commercially useful function (as that term is defined in California Military and Veterans Code § 999 or successor statute) throughout the term of this Agreement. RFB 10850 Liquid Chromatography System and Maintenance Services Page 17 of 31 COUNTY OF SAN DIEGO – REQUEST FOR BIDS (RFB) 10850 SHERIFF’S DEPARTMENT - CRIME LAB LIQUID CHROMATOGRAPHY SYSTEM AND MAINTENANCE SERVICES AGREEMENT

11. Political Activities Prohibited. None of the funds, provided directly or indirectly, under this Agreement shall be used for any political activities or to further the election or defeat of any candidate for public office. Contractor shall not utilize or allow its name to be utilized in any endorsement of any candidate for elected office. Neither this Agreement nor any funds provided hereunder shall be utilized in support of any partisan political activities, or activities for or against the election of a candidate for an elected office.

12. Lobbying. Contractor shall comply with the lobbying ordinances of the County and ensure that its officers and employees comply before any appearance before the County Board of Supervisors. Except as required by this Agreement, none of the funds provided under this Agreement shall be used for publicity or propaganda purposes designed to support or defeat any legislation pending before State and federal legislatures, the Board of Supervisors of the County, or before any other local governmental entity. This provision shall not preclude Contractor from seeking necessary permits, licenses and the like necessary for it to comply with the terms of this Agreement.

13. Americans With Disabilities Act (ADA) 1990. Contractor shall not discriminate against qualified people with disabilities in employment, public services, transportation, public accommodations and telecommunications services in compliance with the Americans with Disabilities Act (ADA) and California Administrative Code Title 24.

14. Religious Activity Prohibited. There shall be no religious worship, instructions, or proselytization as part of or in connection with the performance of this Agreement.

15. Contractor Permits and License. Contractor certifies that it possesses and shall continue to maintain or shall cause to be obtained and maintained, at no cost to County, all approvals, permissions, permits, licenses, and other forms of documentation required for it and its employees to comply with all existing foreign or domestic statutes, ordinances, and regulations, or other laws, that may be applicable to performance of work hereunder. County reserves the right to reasonably request and review all such applications, permits, and licenses prior to the commencement of any work hereunder.

16. Offshore Prohibition. Except where Contractor obtains the County’s prior written approval, Contractor shall perform the work of this Agreement only from or at locations within the United States. Any County approval for the performance of work outside of the United States shall be limited to the specific instance and scope of such written approval, including the types of work and locations involved. Notwithstanding the foregoing, this Section shall not restrict the country or countries of origin of any assets purchased to provide the work hereunder; provided that when such assets are used to provide the work, such assets shall be used only from or at locations within the geographic boundaries of the United States.

17. Equal Opportunity. Contractor shall comply with the provisions of Title VII of the Civil Rights Act of 1964 in that it will not discriminate against any individual with respect to their compensation, terms, conditions, or privileges of employment nor shall Contractor discriminate in any way that would deprive or intend to deprive any individual of employment opportunities or otherwise adversely affect their status as an employee because of such individual’s race, color, religion, sex, national origin, age, handicap, medical condition, sexual orientation or marital status.

18. Non-Discrimination. Contractor shall ensure that services and facilities are provided without regard to ethnic group identification, race, color, nation origin, creed, religion, age, sex, physical or mental disability, political affiliation or marital status in accordance with applicable laws, including, but not limited to, Title VI of the Civil Rights Act of 1964 (42 U.S.C 2000d), Section 162 (a) of the Federal-Aid Highway Act of 1973 (23 U.S.C 324), Section 504 of the Rehabilitation Act of 1973, The Civil Rights Restoration Act of 1987 (P.L. 100-209), Executive Order 12898 (February 11, 1994), Executive Order 13166 (August 16, 2000), Title VII of the Civil Rights Act of 1964 (42 U.S.C. 2000-e), the Age Discrimination Act of 1975 (42 U.S.C. 6101), Article 9.5, Chapter 1, Part 1, Division 2, Title 2 (Section 11135, et seq.) of the California Government Code, Title 9, Division 4, Chapter 6 (Section 10800, et seq.) of the CCR and California Dept. of Social Services Manual of Policies and Procedures (CDSS MPP) Division 19.

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19. Hazardous Materials. Contractor shall comply with all Environmental Laws and all other laws, rules, regulations, and requirements regarding Hazardous Materials, health and safety, notices, and training. Contractor agrees that it will not store any Hazardous Materials at any County facility for periods in excess of ninety (90) days or in violation of the applicable site storage limitations imposed by Environmental Law. Contractor agrees to take, at its expense, all actions necessary to protect third parties, including, without limitation, employees and agents of the County, from any exposure to Hazardous Materials generated or utilized in its performance under this Agreement. Contractor agrees to report to the appropriate governmental agencies all discharges, releases, and spills of Hazardous Materials that are required to be reported by any Environmental Law and to immediately notify the County of it. Contractor shall not be liable to the County for the County’s failure to comply with, or violation of, any Environmental Law. As used in this Section, the term “Environmental Laws” means any and all federal, state or local laws or ordinances, rules, decrees, orders, regulations or court decisions (including the so-called “common law”), including, but not limited to, the Resource Conservation and Recovery Act, relating to hazardous substances, hazardous materials, hazardous waste, toxic substances, environmental conditions or other similar substances or conditions. As used in this Section the term “Hazardous Materials” means any chemical, compound, material, substance or other matter that: (a) is a flammable, explosive, asbestos, radioactive nuclear medicine, vaccine, bacteria, virus, hazardous waste, toxic, overtly injurious or potentially injurious material, whether injurious or potentially injurious by itself or in combination with other materials; (b) is controlled, referred to, designated in or governed by any Environmental Laws; (c) gives rise to any reporting, notice or publication requirements under any Environmental Laws, or (d) is any other material or substance giving rise to any liability, responsibility or duty upon the County or Lessee with respect to any third person under any Environmental Laws.

20. Debarment, Exclusion, Suspension, and Ineligibility. Contractor certifies that, to the best of its knowledge, and except as disclosed to County and acknowledged in writing by County prior to the execution of this Agreement, Contractor, its employees, directors, officers, agents, subcontractors, vendors, consultants, and volunteers: a. Are not presently debarred, excluded, suspended, declared ineligible, voluntarily excluded, or proposed for debarment, exclusion, suspension or ineligibility by any federal, state, or local department or agency; b. Have not within a 3-year period preceding this Agreement been convicted of, or had a civil or administrative judgment rendered against them for, the commission of fraud or a criminal offense or civil action in connection with obtaining, attempting to obtain, or performing a public (federal, State, or local) transaction; violation of federal or State anti-trust statutes or commission of embezzlement, theft, forgery, bribery, falsification or destruction of records, making false statements, receiving stolen property; physical, financial or sexual abuse or misconduct with a patient or client, or medical negligence or malpractice; c. Are not presently indicted or otherwise criminally, civilly or administratively charged by a government entity (federal, state, or local) with commission of any of the offenses enumerated in the paragraph above; d. Have not within a 3-year period preceding this Agreement had one or more public transaction (federal, state, or local) terminated for cause or default.

Contractor shall have an ongoing duty during the term of this Agreement to disclose to the County any occurrence that would prevent Contractor from making the certifications contained in this Section on an ongoing basis. Such disclosure shall be made in writing within five (5) business days of when Contractor discovers or reasonably believes there is a likelihood of such occurrence.

21. Conflicts of Interest. Contractor presently has no interest, including but not limited to other projects or independent agreements, and shall not acquire any such interest, direct or indirect, which would conflict in any manner or degree with the performance of services required to be performed under this Agreement. The Contractor shall not employ any person having any such interest in the performance of this Agreement. Contractor shall not hire County's employees to perform any portion of the work or services provided for herein including secretarial, clerical and similar incidental services except upon the written approval of County. Without such written approval, performance of services under this Agreement by associates or employees of County shall not relieve Contractor from any responsibility under this Agreement

RFB 10850 Liquid Chromatography System and Maintenance Services Page 19 of 31 COUNTY OF SAN DIEGO – REQUEST FOR BIDS (RFB) 10850 SHERIFF’S DEPARTMENT - CRIME LAB LIQUID CHROMATOGRAPHY SYSTEM AND MAINTENANCE SERVICES AGREEMENT

22. California Political Reform Act and Government Code Section 1090, et seq. Contractor acknowledges that the California Political Reform Act (“Act”), Government Code section 81000 et seq., provides that contractors hired by a public agency, such as County may be deemed to be a “public official” subject to the Act if the Contractor advises the agency on decisions or actions to be taken by the agency. The Act requires such public officials to disqualify themselves from participating in any way in such decisions if they have any one of several specified “conflicts of interest” relating to the decision. To the extent the Act applies to Contractor, Contractor shall abide by the Act. In addition, Contractor acknowledges and shall abide by the conflict of interest restrictions imposed on public officials by Government Code section 1090 et seq.

23. Prohibited Agreements. In accordance with Section 67 of the San Diego County Administrative Code, the County shall not contract with, and shall reject any bid or proposal submitted by the person or entities specified below, unless the Board of Supervisors finds that special circumstances exist which justify the approval of such contract: a. Persons employed by the County or of public agencies for which the Board of Supervisors is the governing body; b. Profit making firms or businesses in which employees described in subsection (a) serve as officers, principals, partners or major shareholders; c. Persons who, within the immediately preceding twelve (12) months, came within the provisions of subsection (a), and who (1) were employed in positions of substantial responsibility in the area of service to be performed by the contract, or (2) participated in any way in developing the contract or its service specifications; and d. Profit making firms or businesses in which the former employees described in subsection c. serve as officers, principals, partners or major shareholders.

Contractor certifies it is not a person or entity specified above and that it will promptly notify the County in the event it becomes a person or entity specified above during the term of this Agreement.

24. Indemnity. County shall not be liable for, and Contractor shall defend and indemnify County and the employees and agents of County (collectively “County Parties”), against any and all claims, demands, liability, judgments, awards, fines, mechanics’ liens or other liens, labor disputes, losses, damages, expenses, charges or costs of any kind or character, including attorneys’ fees and court costs (hereinafter collectively referred to as “Claims”), related to this Agreement or the work covered by this Agreement and arising either directly or indirectly from any act, error, omission or negligence of Contractor or its contractors, licensees, agents, servants or employees, including, without limitation, Claims caused by the sole passive negligent act or the concurrent negligent act, error or omission, whether active or passive, of County Parties. Contractor shall have no obligation, however, to defend or indemnify County Parties from a Claim if it is determined by a court of competent jurisdiction that such Claim was caused by the sole negligence or willful misconduct of County Parties.

Without limiting the foregoing, Contractor’s defense and indemnity obligations under this Section shall specifically apply to any claim, suit, proceeding, demand, liability, loss, damage or expense (including but not limited to attorneys’ fees) arising from or relating to a claim that any work performed pursuant to this Agreement infringes a patent, copyright, moral right, trademark, trade secret, or other intellectual property right of a third party. Without limiting the generality of the foregoing, if any portion of any the same or County’s use of the same is, or in Contractor’s or County’s opinion is likely to be, held to infringe the rights of any third party, Contractor shall at its expense either (i) procure the right for County to use the infringing item free of any liability or expense to County to the full extent contemplated by this Agreement; or (ii) replace it with a non-infringing equivalent reasonably satisfactory to County. Without limiting the County’s other rights and Contractor’s obligations under this Section, County shall have the right to employ counsel at its own expense for, and participate in the defense of, any claim.

25. Insurance. Prior to execution of this Agreement, Contractor must obtain at its own cost and expense, and keep in force and effect during the term of this Agreement, including all extensions, the insurance specified in Exhibit B, Insurance Requirements, attached hereto.

RFB 10850 Liquid Chromatography System and Maintenance Services Page 20 of 31 COUNTY OF SAN DIEGO – REQUEST FOR BIDS (RFB) 10850 SHERIFF’S DEPARTMENT - CRIME LAB LIQUID CHROMATOGRAPHY SYSTEM AND MAINTENANCE SERVICES AGREEMENT

26. Maintenance of Records. Contractor shall maintain and keep available all records within the County of San Diego for a minimum of three (3) years from the ending date of this Agreement unless a longer period is specified in the Statement of Work or unless County agrees in writing to an earlier disposition.

27. Audit Right. Pursuant to California Government Code Section 8546.7, the parties acknowledge that every contract involving the expenditure of public funds in excess of $10,000 shall be subject to audit by the State Auditor.

Authorized federal, State and County representatives shall have the right to monitor, assess, and evaluate Contractor’s performance pursuant to this Agreement, said monitoring, assessments, or evaluations to include but not be limited to audits, inspection of premises, reports, and interviews of project staff and participants.

At any time during normal business hours and as often as County may deem necessary, Contractor shall make available to County, State or federal officials for examination all of its records with respect to all matters covered by this Agreement and will permit County, State or federal officials to audit, examine and make excerpts or transcripts from such records, and to make audits of all invoices, materials, payrolls, records of personnel, information regarding clients receiving services, and other data relating to all matters covered by this Agreement. If an audit is conducted, it will be done in accordance with generally accepted government auditing standards as described in Government Auditing Standards, published for the United States General Accounting Office.

28. Termination for Convenience. The County may, by written notice stating the extent and effective date, terminate this Agreement for convenience in whole or in part, at any time. The County shall pay the Contractor, as full compensation for work performed in accordance with the terms of this Agreement until such termination: a. The unit or pro rata price for any delivered and accepted portion of the work. b. A reasonable amount, as costs of termination, not otherwise recoverable from other sources by the Contractor as approved by the County, with respect to the undelivered or unaccepted portion of the work, provided compensation hereunder shall in no event exceed the total price. c. In no event shall the County be liable for any loss of profits on the work or portion thereof so terminated. d. County’s termination of this Agreement for convenience shall not preclude County from taking any action in law or equity against Contractor for: i. Improperly submitted claims, or ii. Any failure to perform the work in accordance with the Statement of Work, or iii. Any breach of any term of condition of the Agreement, or iv. Any actions under any warranty, express or implied, or v. Any claim of professional negligence, or vi. Any other matter arising from or related to this Agreement, whether known, knowable or unknown before, during or after the date of termination.

29. Termination for Default. The County may, by written notice of default to the Contractor, terminate this Agreement in whole or in part, should the Contractor fail to make satisfactory progress, fail to perform within the time specified, or fail to deliver in strict conformance to specifications or requirements set forth herein. In the event of such termination, the County reserves the right to purchase or obtain the supplies or services elsewhere and the Contractor shall be liable for the difference between the prices set forth in this Agreement and the actual cost thereof to the County. In such case, the prevailing market price shall be considered to be the fair repurchase price. The rights and remedies of County provided in this Section shall not be exclusive and are in addition to any other rights and remedies provided by law or under this Agreement.

If, after notice of termination of this Agreement under the provisions of this clause, it is determined for any reason that the Contractor was not in default under the provisions of this clause, the rights and obligations of the parties shall be the same as if the notice of termination had been issued pursuant to Section 28, Termination for Convenience.

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30. Full Cost Recovery of Investigation and Audit Costs. Contractor shall reimburse County (by direct payment or County withholding of payment, at County’s sole discretion) for all direct and indirect expenditures incurred in conducting an audit/investigation when Contractor is found in violation (material breach) of the terms of the Agreement.

31. Disallowance. In the event the Contractor receives payment for work under Agreement that is later disallowed by the County, the Contractor shall promptly refund the disallowed amount to the County on request, or at its option, the County may offset the amount disallowed from any payment due to the Contractor under any contract with the County.

32. Warranty. Contractor agrees that any goods and/or services furnished under this Agreement shall be covered by the most favorable commercial warranties Contractor gives to any of its customers for the same or substantially similar goods and/or services. Any warranties so provided shall supplement, and shall not limit or reduce, any rights afforded to County by any other provision of this Agreement or by any applicable Uniform Commercial Code warranties.

33. Assignment. This Agreement is assignable by County. Except as to any payment due hereunder, this Agreement is not assignable by Contractor without written approval of County.

34. Delivery. Unless otherwise specified in writing in this Agreement, all shipments shall be F.O.B. point of destination. County shall not be liable for freight or handling charges except as stated in this Agreement. Transportation receipts, if allowed under the Agreement, must accompany invoice.

35. Changes. The Director of Purchasing and Contracting or designee is the only County official authorized to modify this Agreement. The County may at any time, by written order, make changes within the general scope of this Agreement ("Change Order"). If any Change Order causes an increase or decrease in the cost or time required for the performance of the work under this Agreement, an equitable adjustment shall be made to the price, delivery schedule, or both. No other modification of this Agreement shall be valid unless it is in writing and signed by both parties.

36. Waiver. The waiver by one party of the performance of any term, provision, covenant or condition shall not invalidate this Agreement, nor shall it be considered as a waiver by such party of any other term, provision, covenant or condition.

37. Governing Law. This Agreement shall be governed, interpreted, construed and enforced in accordance with the laws of the State of California.

38. Timeliness. Time is of the essence for each provision of this Agreement.

IN WITNESS WHEREOF, County and Contractor have executed this Agreement effective as of the date of the last signature below.

COUNTY OF SAN DIEGO [#CONTRACTOR NAME] JOHN M. PELLEGRINO, Director Department of Purchasing and Contracting

By: ______By: ______[#Name and Title] [#Name and Title]

Date: ______Date: ______

RFB 10850 Liquid Chromatography System and Maintenance Services Page 22 of 31 COUNTY OF SAN DIEGO – REQUEST FOR BIDS (RFB) 10850 SHERIFF’S DEPARTMENT - CRIME LAB LIQUID CHROMATOGRAPHY SYSTEM AND MAINTENANCE SERVICES EXHIBIT A – STATEMENT OF WORK

I. SCOPE OF SERVICES Contractor shall provide a complete bundled system for the efficient analysis of both known and unknown controlled substances. The Liquid Chromatography system shall be the listed brand/model or equivalent. Contractor shall provide the following: Make/Model: Waters Acquity RDa System "or equal" A. Liquid Chromatography (LC) system: 1. Must include a temperature-controlled compartment for the LC column with a temperature range of 20-90 °C. 2. Must be capable of accurate binary solvent blending at pressures up to 18,000 psi. 3. System must be capable of operating at flow ranges of 0.001 to 2.000mL/min with inject-to-inject cycle times of ≤15 seconds. Flow accuracy of ±1% at flow rate of 0.500 mL/min. 4. Utilizes a Flow-Through Needle system (FTN) for injection volumes of 0.1-10 Ul. 5. Must include UPLC HSS T3 1.8 um, 2.1x100mm LC column or equivalent. 6. The system must support leak sensing with full 96-hour diagnostic data displayed through console software. Drips must be managed through a drip tray, which directs all leaks to either the front or rear of the instrument, and then into a suitable waste container. 7. System includes sample manager (autosampler) capable of sampling from well plates or vials with sample capacity minimum of 96 x 2.00mL vials. a. Sample compartment is temperature controlled with a range of 4.0-40.0 °C. b. Samples can be added to the autosampler system during a run. c. The sample manager heat time must be≤ 30 min from room temperature to 40°C. The sample manager cool time must be ≤ 60 min from room temperature to 4°C. 8. System contains an integrated injection needle wash. 9. System is equipped with active wash system to flush rear of high-pressure seals and plunger. 10. The pump must have an integrated degasser, which operates up to the maximum flow rate, with an independent channel for each solvent. The degasser must support additional channels for the sample manager. The total solvent volume must be less than 480 µL. B. Time of Flight Mass Spectrometer (TOF) 1. Equipped with a high stability TOF mass analyzer a. Mass measurement accuracy of <2.5 ppm b. Utilizes lock mass solutions for mass scale calibration accuracy of <1 ppm over a mass range of 50-2000 m/z 2. Must be capable of full scan with minimum mass ranges of: a. Positive ion mode: 50-2000 m/z or 400-7000 m/z b. Negative ion mode: 50-2000 m/z or 400-5000 m/z

RFB 10850 Liquid Chromatography System and Maintenance Services Page 23 of 31 COUNTY OF SAN DIEGO – REQUEST FOR BIDS (RFB) 10850 SHERIFF’S DEPARTMENT - CRIME LAB LIQUID CHROMATOGRAPHY SYSTEM AND MAINTENANCE SERVICES EXHIBIT A – STATEMENT OF WORK

3. The system is able to collect: Full Scan Mass Spectra (MS) Or Simultaneous full scan MS and fragment ion MS (Full Scan with Fragmentation) 4. The TOF has a pre-optimized ionization source which requires no physical user-adjustment for consistent performance. 5. The TOF is able to self-diagnose faults. If a fault occurs with the instrument, the software needs to display information that provides step-by-step guidance to fix the fault. 6. Routine maintenance can be performed without venting the TOF or replacing the sample cone assembly or equivalent. 7. The TOF mass analyzer has a small foot print no bigger than 80 (H) x 40 (W) x 80 (D) cm (H W x D). 8. The TOF can be connected to a single-phase mains electricity supply that has a nominal voltage within the range 100 to 127 Volts, without the need for much laboratory preparation including an additional transformer within the laboratory. 9. System already demonstrated to be capable of the analysis of seized drug unknown samples. C. Instrument Software Requirements 1. Must include reliable processing and instrument control software for setting up sequence/run tables, tuning, method configurations, real-time system status, and diagnostics. a. Automated workflow for routine analysis data processing b. Customizable, automated reporting c. Software is capable of communicating with common Laboratory Information Management Systems (LIMS) for data analysis and reporting functionality. 2. Must include spectral interpretation and classification data for the identification of unknowns. 3. Software must include a mass library of pharmaceutical and controlled substances for forensic purposes. a. Software must allow for building custom, in-house libraries. 4. Software must include drug discovery tools to access chemical databases to identify unknown compounds from analysis. 5. PC system capable of integration into Sheriff Network (minimum Windows 10, 16 GB memory, modern CPU processor) a. Hard drive storage capacity for modern TOF data storage (2 terabytes or more) CI. Nitrogen Generator 1. Must include multi-stage purification system capable of nitrogen gas purity of 99.5%. 2. Generator capable of variable pressure up to 116 psi with max flow rate of 35 L/min 3. Generator system must be Phthalate and Butylated hydroxytoluene (BHT) free. 4. Generator must be capable of supporting high performance LC-TOF instruments. 5. Generator must be able to utilize a 120V outlet. CII. Consumables 1. Must include 1 pack of 50 0.2µm 25mm syringe filters. 2. Must include ten 5.75 glass Pasteur pipettes.

RFB 10850 Liquid Chromatography System and Maintenance Services Page 24 of 31 COUNTY OF SAN DIEGO – REQUEST FOR BIDS (RFB) 10850 SHERIFF’S DEPARTMENT - CRIME LAB LIQUID CHROMATOGRAPHY SYSTEM AND MAINTENANCE SERVICES EXHIBIT A – STATEMENT OF WORK

3. Must include two 2-mm latex pipette bulbs. 4. Must include at least four class A 10mL volumetric pipettes. 5. Must contain one kit of mass detector calibration solution containing Leu-enkephalin, Caffeine, and Pentenasulfonic Acid to compensate for mass shift to due temperature changes. F. Additional Requirements 1. Training a. On-site training by an applications scientist, until the needs of the department are met. b. Training shall be provided during normal business hours Monday through Friday from 8:00 AM to 5:00 PM (San Diego Time) excluding County Holidays. 2. Installation a. Complete, professional installation of the LC-TOF system. b. The system will be configured with a method for the analysis of seized drugs. 3. Quality Assurance a. Include a Declaration of Conformity to ensure each system component meets or exceeds performance specifications. b. System must reliably function on a daily basis with minimal downtime. 4. Maintenance Contract a. Unlimited number of support calls, responds on site within 72 hours with factory-certified engineer. Services include the following at no additional cost: i. Engineer labor and travel ii. Planned maintenance visits iii. Performance verified parts iv. One scheduled preventative maintenance visit per year v. Technical reviews using call history vi. Software updates and notifications vii. E-support online technical resources viii. Priority status for Technical Support Inquiries, response within two hours ix. Contractor shall provide written details of all service performed on the instrument x. Contractor shall provide on-site service during normal business hours Monday through Friday from 8:00 AM to 5:00 PM (San Diego Time) excluding County Holidays b. This is to be a single vendor contract. c. This Contract will be in effect for no less than two years from the installation date.

II. OTHER REQUIREMENTS

A. Where the material, design or construction of the equipment is not specified, the quality of such equipment shall be, at a minimum, rated for commercial use.

B. Only new equipment is accepted. Equipment that has been used in any way, refurbished, reconditioned, or grey market goods, etc., is absolutely unacceptable and will be rejected upon delivery.

RFB 10850 Liquid Chromatography System and Maintenance Services Page 25 of 31 COUNTY OF SAN DIEGO – REQUEST FOR BIDS (RFB) 10850 SHERIFF’S DEPARTMENT - CRIME LAB LIQUID CHROMATOGRAPHY SYSTEM AND MAINTENANCE SERVICES EXHIBIT A – STATEMENT OF WORK

C. Equipment shall be provided with model-specific assembly and maintenance manual.

D. Equipment shall be suitable and ready for use by end-user without professional installation required, unless specified as "required assembly by vendor."

E. Lead time for delivery shall be no more than 30 days from notice of award.

F. For “or equal” brands, offeror must submit a request for pre-approval at least 5 days before the bid due date and time, including sufficient information for the County to evaluate the proposed equal item. It is the Contractor’s responsibility to provide sufficient information.

III. WARRANTY AND RETURNS

A. A commercial warranty shall be included, and shall be the greater of the manufacturer’s warranty, vendor- provided warranty, or the minimum warranty specified in equipment specifications section.

B. Equipment shall be provided with warranty information with contact phone number for support and services per warranty.

C. The County shall be given credit for damaged and returned items within five (5) business days.

D. There shall be no restocking fee or other charges for returns of damaged or incorrect items.

IV. DELIVERY AND SERVICE LOCATION

A. Deliveries are by appointment only and shall only occur between the hours of 7:00am and 2:00pm San Diego Time, Monday – Friday (Excluding County observed Holidays).

B. Contractor shall make delivery to the following location: San Diego County Sheriff's Department Crime Laboratory 5590 Overland Avenue San Diego, CA 92123

RFB 10850 Liquid Chromatography System and Maintenance Services Page 26 of 31 COUNTY OF SAN DIEGO – REQUEST FOR BIDS (RFB) 10850 SHERIFF’S DEPARTMENT - CRIME LAB LIQUID CHROMATOGRAPHY SYSTEM AND MAINTENANCE SERVICES EXHIBIT B – INSURANCE REQUIREMENTS

INSURANCE REQUIREMENTS FOR CONTRACTORS Without limiting Contractor’s indemnification obligations to County, Contractor shall provide at its sole expense and maintain for the duration of this contract, or as may be further required herein, insurance against claims for injuries to persons or damages to property which may arise from or in connection with the performance of the work hereunder and the results of the work by the Contractor, his agents, representatives, employees or subcontractors.

1. Minimum Scope of Insurance Coverage shall be at least as broad as: A. Commercial General Liability, Occurrence form, Insurance Services Office form CG0001.

B. Automobile Liability covering all owned, non owned, hired auto Insurance Services Office form CA0001.

C. Workers’ Compensation, as required by State of California and Employer’s Liability Insurance.

2. Minimum Limits of Insurance Contractor shall maintain limits no less than: A. Commercial General Liability including Premises, Operations, Products and Completed Operations, Contractual Liability, and Independent Contractors Liability: $2,000,000 per occurrence for bodily injury, personal injury and property damage. The General Aggregate limit shall be $4,000,000.

B. Automobile Liability: $1,000,000 each accident for bodily injury and property damage.

C. Employer’s Liability: $1,000,000 each accident for bodily injury or disease. Coverage shall include waiver of subrogation endorsement in favor of County of San Diego.

If the contractor maintains broader coverage and/or higher limits than the minimums shown above, the County requires and shall be entitled to the broader coverage and/or higher limits maintained by the Contractor. As a requirement of this contract, any available insurance proceeds in excess of the specified minimum limits and coverage stated above, shall also be available to the County of San Diego.

3. Self-Insured Retentions Any self-insured retention must be declared to and approved by County Risk Management. At the option of the County, either: the insurer shall reduce or eliminate such self-insured retentions as respects the County, the members of the Board of Supervisors of the County and the officers, agents, employees and volunteers; or the Contractor shall provide a financial guarantee satisfactory to the County guaranteeing payment of losses and related investigations, claim administration, and defense expenses.

4. Other Insurance Provisions The insurance policies are to contain, or be endorsed to contain, the following provisions: A. Additional Insured Endorsement The County of San Diego, the members of the Board of Supervisors of the County and the officers, agents, employees and volunteers of the County, individually and collectively are to be covered as additional insureds on the General Liability policy with respect to liability arising out of work or operations performed by or on behalf of the Contractor including materials, parts, or equipment furnished in connection with such work or operations and automobiles owned, leased, hired or borrowed by or on behalf of the Contractor. General Liability coverage can be provided in the form of an endorsement to the Contractor’s insurance (at least as broad as ISO from CG 2010 11 85 or both CG 2010, CG 2026, CG 2033, or CG 2038; and CG 2037 forms if later revisions used).

B. Primary Insurance Endorsement For any claims related to this contract, the Contractor’s insurance coverage, including any excess liability policies, shall be primary insurance at least as broad as ISO CG 2001 04 13 as respects the County, the members of the Board of Supervisors of the County and the officers, agents, employees and volunteers of the County, individually and

RFB 10850 Liquid Chromatography System and Maintenance Services Page 27 of 31 COUNTY OF SAN DIEGO – REQUEST FOR BIDS (RFB) 10850 SHERIFF’S DEPARTMENT - CRIME LAB LIQUID CHROMATOGRAPHY SYSTEM AND MAINTENANCE SERVICES EXHIBIT B – INSURANCE REQUIREMENTS

collectively. Any insurance or self-insurance maintained by the County, its officers, employees, or volunteers shall be excess of the Contractor’s insurance and shall not contribute with it.

C. Notice of Cancellation Each insurance policy required above shall state that coverage shall not be canceled, except with notice to the County.

D. Severability of Interest Clause Coverage applies separately to each insured, except with respect to the limits of liability, and that an act or omission by one of the named insureds shall not reduce or avoid coverage to the other named insureds.

General Provisions

5. Qualifying Insurers All required policies of insurance shall be issued by companies which have been approved to do business in the State of California by the State Department of Insurance, and which hold a current policy holder’s alphabetic and financial size category rating of not less than A, VII according to the current Best’s Key Rating guide, or a company of equal financial stability that is approved in writing by County Risk Management.

6. Evidence of Insurance Prior to commencement of this Contract, but in no event later than the effective date of the Contract, Contractor shall furnish the County with a copy of the policy declaration and endorsement pages along with the certificates of insurance and amendatory endorsements effecting coverage required by this clause. Policy declaration and endorsement pages shall be included with renewal certificates and amendatory endorsements submissions and shall be furnished to County within thirty days of the expiration of the term of any required policy. Contractor shall permit County at all reasonable times to inspect any required policies of insurance.

7. Failure to Obtain or Maintain Insurance; County’s Remedies Contractor’s failure to provide insurance specified or failure to furnish certificates of insurance and amendatory endorsements or failure to make premium payments required by such insurance shall constitute a material breach of the Contract, and County may, at its option, terminate the Contract for any such default by Contractor.

8. No Limitation of Obligations The foregoing insurance requirements as to the types and limits of insurance coverage to be maintained by Contractor, and any approval of said insurance by the County are not intended to and shall not in any manner limit or qualify the liabilities and obligations otherwise assumed by Contractor pursuant to the Contract, including, but not limited to, the provisions concerning indemnification.

9. Review of Coverage County retains the right at any time to review the coverage, form and amount of insurance required herein and may require Contractor to obtain insurance reasonably sufficient in coverage, form and amount to provide adequate protection against the kind and extent of risk which exists at the time a change in insurance is required.

10. Self-Insurance Contractor may, with the prior written consent of County Risk Management, fulfill some or all of the insurance requirements contained in this Contract under a plan of self-insurance. Contractor shall only be permitted to utilize such self-insurance if in the opinion of County Risk Management, Contractor’s (i) net worth, and (ii) reserves for payment of claims of liability against Contractor, are sufficient to adequately compensate for the lack of other insurance coverage required by this Contract. Contractor’s utilization of self-insurance shall not in any way limit liabilities assumed by Contractor under the Contract.

RFB 10850 Liquid Chromatography System and Maintenance Services Page 28 of 31 COUNTY OF SAN DIEGO – REQUEST FOR BIDS (RFB) 10850 SHERIFF’S DEPARTMENT - CRIME LAB LIQUID CHROMATOGRAPHY SYSTEM AND MAINTENANCE SERVICES EXHIBIT B – INSURANCE REQUIREMENTS

11. Subcontractors’ Insurance Contractor shall require and verify that all subcontractors maintain insurance meeting all the requirements stated herein, and Contractor shall ensure that County is an additional insured on insurance required from subcontractors. Such Additional Insured endorsement shall be attached to the certificate of insurance in order to be valid and on a form at least as broad as ISO from CG 2010 11 85 or both CG 2010, CG 2026, CG 2033, or CG 2038; and CG 2037 forms if later revisions used. If any sub contractor’s coverage does not comply with the foregoing provisions, Contractor shall defend and indemnify the County from any damage, loss, cost, or expense, including attorneys’ fees, incurred by County as a result of subcontractor’s failure to maintain required coverage.

12. Waiver of Subrogation Contractor hereby grants to County a waiver of their rights of subrogation which any insurer of Contractor may acquire against County by virtue of the payment of any loss. Contractor agrees to obtain any endorsement that may be necessary to affect this waiver of subrogation. The Workers’ Compensation policy shall be endorsed with a waiver of subrogation in favor of the County for all work performed by the Contractor, its employees, agents and subcontractors.

RFB 10850 Liquid Chromatography System and Maintenance Services Page 29 of 31 COUNTY OF SAN DIEGO – REQUEST FOR BIDS (RFB) 10850 SHERIFF’S DEPARTMENT - CRIME LAB LIQUID CHROMATOGRAPHY SYSTEM AND MAINTENANCE SERVICES EXHIBIT C: PAYMENT SCHEDULE

THE PAYMENT SCHEDULE IS POSTED TO BUYNET

AS A SEPARATE EXCEL ATTACHMENT

Bidders should be sure to read and complete, in its entirety, the Payment Schedule.

Award will be made to the lowest, responsive, responsible bidder based on the total price for the entire contract term as stated on the Payment Schedule.

RFB 10850 Liquid Chromatography System and Maintenance Services Page 30 of 31 COUNTY OF SAN DIEGO – REQUEST FOR BIDS (RFB) 10850 SHERIFF’S DEPARTMENT - CRIME LAB LIQUID CHROMATOGRAPHY SYSTEM AND MAINTENANCE SERVICES

Payment Terms.

Payment terms are, unless otherwise specified by County, thirty (30) days from the later of: (i) performance of work under the Agreement entitling Contractor to payment, or (ii) County receipt of a correct and substantiated invoice. Payment shall be deemed to have been made on the date the County submits electronic payment or mails a warrant or check. Contractor may offer County a prompt payment discount or other more favorable payment terms at any time during the term of the Agreement, which the County may accept or not accept at its discretion. If Contractor qualifies as a Small Business or Veteran Owned Business, County may authorize more favorable payment terms in accordance with Board of Supervisor’s Policy B-53 Small Business Policy B-39a Veteran Owned Business (VOB) and Disabled Veterans Business Enterprise (DVBE) Program.

Prices

Prices shall be fixed during the performance of the Agreement and not subject to adjustment except as explicitly set forth in this Agreement. Bidder accepts the risk of any changes to bidder’s costs during the term of the Agreement.

Estimated Quantities

Any estimated quantities are provided solely for evaluation of bids and are not guarantees by the County. The successful bidder(s) shall not be entitled to price adjustment or other relief on the basis of any variance from estimated quantities.

RFB 10850 Liquid Chromatography System and Maintenance Services Page 31 of 31 Quotation

Quote No. Create Date Delivery Time Page Jorge Pena 3545728 04/16/2021 5 Weeks 1 of 6 County of San Diego 5255 Mount Etna Dr Contact Phone no. Valid to San Diego CA 92117-6912 Alexander Soloviev 918-613-4559 06/15/2021

To place an order: Visit www.agilent.com/store to place online order using a purchase order or credit card and track your order status. Product Email FAX Consumables [email protected] 302-633-8901 Genomics [email protected] 512-321-3128 Pathology [email protected] 800-566-3256 Instruments [email protected] 302-633-8953 1-800-227-9770 Option 1

Unit Extended List Discount Net Item Product/Description Qty/Unit Price Amount Price

1000 G7120A 1.000 EA 49,606.01 USD 27,283.31- 22,322.70

1290 Infinity II High Speed Pump. Power range 1300 bar and 5ml/min flow, binary high pressure mixing, lowest delay, highest precision and accuracy. Includes Active Seal Wash, Tool Kit, Solvent Cabinet, bottles and ISET.

With the following configuration: Select bundled column : RRHD Eclipse Plus C18,2.1x50mm Delete ISET (895) : Delete ISET Manual DVD for 1220/1260/1290 : DVD included Ship-to Country : USA RRHD Eclipse Plus C18,2.1x50mm,1.8um Delete ISET Installation (44K) Training (44P)

Special discount of 55.00 % is applied.

2000 G7167B 1.000 EA 38,513.00 USD 21,182.15- 17,330.85

1290 Infinity Multisampler up to 1300 bar for well plates and individual sample containers. Includes 1 sample drawer, 2x 54-vial containers, a needle flush port, 40 uL metering device, 20 uL loop. (for Dual-needle instruments metering is 100 ul and loop size is 100 ul).

With the following configuration: Type of SW license : MassHunter System Driver Add thermostat (101) : InfLab sample thermostat incl. Dual-height drawer kit (132) : 2 Ship-to Country : USA Agilent InfinityLab Sample Thermostat Dual-height drawer (2H) Installation (44K)

Special discount of 55.00 % is applied.

3000 G7116B 1.000 EA 9,249.00 USD 5,086.95- 4,162.05 Quotation

Quote No. Create Date Delivery Time Page

3545728 04/16/2021 5 Weeks 2 of 6 Jorge Pena County of San Diego Contact Phone no. Valid to 5255 Mount Etna Dr Alexander Soloviev 918-613-4559 06/15/2021 San Diego CA 92117-6912 To place an order: Visit www.agilent.com/store to place online order using a purchase order or credit card and track your order status. Product Email FAX Consumables [email protected] 302-633-8901 Genomics [email protected] 512-321-3128 Pathology [email protected] 800-566-3256 Instruments [email protected] 302-633-8953 1-800-227-9770 Option 1

Unit Extended List Discount Net Item Product/Description Qty/Unit Price Amount Price

1290 Infinity II Multicolumn Thermostat. Capacity up to 8 columns, temperature range 4 deg C. to 110 deg C. Includes 1.6uL Quick-Connect Heat Exchanger, Quick-Connect fitting and two Quick-Turn fittings. Valve drive optional.

With the following configuration: Ship-to Country : USA Valve drive for 1290 MCT Installation (44K)

Special discount of 55.00 % is applied.

4000 G4231C 1.000 EA 8,331.00 USD 4,582.05- 3,748.95

2pos/6port valve head, 1300bar

With the following configuration: Kap kit QCHE G4231C (005) : selected Ship-to Country : USA 2/6 cap kit 0.12 mm ID, incl. QC-HE Installation (44K)

Special discount of 55.00 % is applied.

5000 G6230BA 1.000 EA 334,724.00 USD 184,098.20- 150,625.80

Accurate Mass TOF LC/MS System with AJS

With the following configuration: Ship-to Country : USA Add Laser Printer Quotation

Quote No. Create Date Delivery Time Page

3545728 04/16/2021 5 Weeks 3 of 6 Jorge Pena County of San Diego Contact Phone no. Valid to 5255 Mount Etna Dr Alexander Soloviev 918-613-4559 06/15/2021 San Diego CA 92117-6912 To place an order: Visit www.agilent.com/store to place online order using a purchase order or credit card and track your order status. Product Email FAX Consumables [email protected] 302-633-8901 Genomics [email protected] 512-321-3128 Pathology [email protected] 800-566-3256 Instruments [email protected] 302-633-8953 1-800-227-9770 Option 1

Unit Extended List Discount Net Item Product/Description Qty/Unit Price Amount Price

Installation (44K) Introduction (44L) 1 Year SW Update/Phone Assist (44W) Training (44P) 1YR PC Repair Recovery Service (0TP)

Special discount of 55.00 % is applied.

6000 G1953A 1.000 EA 33,133.00 USD 18,223.15- 14,909.85

PEAK Nitrogen Generator System, including install. For model specific information, consult ELSA N2 generator ordering guide. For extended warranty and support service, contact PEAK directly. www.peakscientific.com

With the following configuration: Ship-to Country : USA Peak Genius XE 35 Nitrogen Generator120V Installation (44K)

Special discount of 55.00 % is applied.

6500 G3876CA 1.000 EA 17,205.00 USD 9,462.75- 7,742.25

LC/MS QTOF Standalone Forensics Toxicology PCDL.

With the following configuration: Ship-to Country : USA

Generic Checkout Test Mix + Column Installation (44K) Introduction (44L)

Special discount of 55.00 % is applied.

7000 H2149A 1.000 EA 11,059.00 USD 2,764.75- 8,294.25 Quotation

Quote No. Create Date Delivery Time Page

3545728 04/16/2021 5 Weeks 4 of 6 Jorge Pena County of San Diego Contact Phone no. Valid to 5255 Mount Etna Dr Alexander Soloviev 918-613-4559 06/15/2021 San Diego CA 92117-6912 To place an order: Visit www.agilent.com/store to place online order using a purchase order or credit card and track your order status. Product Email FAX Consumables [email protected] 302-633-8901 Genomics [email protected] 512-321-3128 Pathology [email protected] 800-566-3256 Instruments [email protected] 302-633-8953 1-800-227-9770 Option 1

Unit Extended List Discount Net Item Product/Description Qty/Unit Price Amount Price

Method and Application Consulting On-site consulting for a maximum of 4 participants. Certificates and manuals not included.

With the following configuration: Ship-to Country : USA Three Day On-site (Includes Travel)

Special discount of 25.00 % is applied.

8000 SYS-LM-TOF 1.000 EA 49,560.00 USD 14,868.00- 34,692.00

Support for: LCMS TOF System

With the following configuration: Ship-to Country : USA

CrossLab Silver - 2yrs total

Promotion discount 5.00 %. Special discount of 25.00 % is applied.

Gross Amount : $ 551,380.01 Total Discount : $ 287,551.31 Net Amount : $ 263,828.70

Total : $ 263,828.70 Quotation

Quote No. Create Date Delivery Time Page

3545728 04/16/2021 5 Weeks 5 of 6 Jorge Pena County of San Diego Contact Phone no. Valid to 5255 Mount Etna Dr Alexander Soloviev 918-613-4559 06/15/2021 San Diego CA 92117-6912 To place an order: Visit www.agilent.com/store to place online order using a purchase order or credit card and track your order status. Product Email FAX Consumables [email protected] 302-633-8901 Genomics [email protected] 512-321-3128 Pathology [email protected] 800-566-3256 Instruments [email protected] 302-633-8953 1-800-227-9770 Option 1

Ask about our attractive payment options and how we can help you acquire the latest innovations while minimizing the upfront costs. Contact your Agilent sales representative today or visit us online at www.agilent.com/en/technology/agilent-financial-solutions to learn why more and more labs are choosing these flexible payment plans and identify which works best for you.

To place an order: Visit www.agilent.com/store to place online orders using a purchase order or credit card and track your order status. Product Email FAX Consumables [email protected] 302-633-8901 Genomics [email protected] 512-321-3128 Pathology [email protected] 800-566-3256 Instruments [email protected] 302-633-8953 1-800-227-9770 Option 1

To place an order, the following information is required: · Purchase order number or credit card, delivery date, ship to, invoice to, end user, and quote number. . GSA customers please provide GSA contract #.

EXCLUSIVE OFFERS FOR NEW INSTRUMENT CUSTOMERS, go to www.agilent.com/chem/exclusiveoffers TO CHECK THE STATUS OF AN ORDER: 1) Visit agilent.com/store to check the status of your order. 2) Call 1-800-227-9770 (option 1) any weekday between 8 am and 8 pm Eastern time, in the U.S., Canada & Puerto Rico. You will need to know the purchase order or credit card number the order was placed on.

TERMS AND CONDITIONS:

· Pricing: Web prices are provided only for the U.S. in U.S.dollars. All phone prices are in local currency and for end use. Applicable local taxes are applied. · All Sales Tax is subject to change at the time of order. · Shipping and Handling Charges: Orders with a value less than $4000 or those requiring special services such as overnight delivery may be subject to additional shipping & handling fees. Some of these charges may be avoided by ordering via the Web · Payment Terms: Net 30 days from invoice date, subject to credit approval. * Quotation Validity: This quotation is valid for 60 days unless otherwise indicated. * Warranty period for instrumentation is 1 year. The Warranty period for columns and consumables is 90 days. Visit www.agilent.com/chem · For Training course information and registration including e-Seminars, select Education. · For Literature, Application notes, and other information, select Library. · For Online Technical Support including the Technical Support Assistant and Frequently Asked Questions, select Technical Support.

It is Agilent Technologies intent to ship product at the earliest available date unless specified otherwise. Quotation

Quote No. Create Date Delivery Time Page

3545728 04/16/2021 5 Weeks 6 of 6 Jorge Pena County of San Diego Contact Phone no. Valid to 5255 Mount Etna Dr Alexander Soloviev 918-613-4559 06/15/2021 San Diego CA 92117-6912 To place an order: Visit www.agilent.com/store to place online order using a purchase order or credit card and track your order status. Product Email FAX Consumables [email protected] 302-633-8901 Genomics [email protected] 512-321-3128 Pathology [email protected] 800-566-3256 Instruments [email protected] 302-633-8953 1-800-227-9770 Option 1

The sale of Agilent Products and Services referenced in this quotation is subject to the then current version of Agilent's Terms of Sale, and any Supplemental Terms or Occasional Reseller Terms of Sale or other applicable terms referenced herein. The sale of Microplates Products shall be subject to Microplates Terms of Sale and any Supplemental Terms or other applicable terms referenced herein. The sale of Microplates Tooling Products shall be subject to Microplates Tooling Terms of Sale and any Supplemental Terms or other applicable terms referenced herein. All of the above "Terms" as applicable. A copy of the Terms is either attached or has been previously provided to you. Please contact us if you have not received a copy or require an additional copy. If you have a separate agreement in effect with Agilent covering the sale of Products and Services referenced in this quotation, the terms of that agreement will take precedence for those Products and Services. Agilent expressly objects to any different or additional terms in your purchase/sales order documentation, unless agreed to in writing by Agilent. Products and Services availability dates are estimated at the time of the quotation. Actual delivery dates or delivery windows will be specified at the time Agilent acknowledges and accepts your purchase order. The above conditions shall apply to the fullest extent permitted by the law. You may have other statutory or legal rights available. Commodities, technology or software exported from the United States of America ("U.S.") or from other exporting countries will be subject to the U.S. Export Administration Regulations and all exporting countries' export laws and regulations. Diversion contrary to U.S. law and the applicable export laws and regulations is prohibited. Agilent Technologies Inc. Tel: 1-800-227-9770 opt 1, then 5 2850 Centerville Road Fax: 1-302-633-8954 Wilmington DE 19808-1610 Email: [email protected] United States Website: www.agilent.com/chem

Delivery Site: County of San Diego Sheriffs Dept Quotation Number: Quotation Date: 5255 Mount Etna Dr 5000895160 April 21, 2021 SAN DIEGO CA 92117-6912 USA Tax ID: Coverage Period: Payment Terms: 06/01/2022 - 05/31/2026 Net 30 Days Jorge Pena [email protected] Quotation Expiration Date: +18582856300 All quoted prices are valid for 90 days from quotation date. Invoice To: County of San Diego Direct Inquiries to: Sheriffs Dept Contact Name: Peter Ott 5255 Mount Etna Dr Contact E-mail: [email protected] SAN DIEGO CA 92117-6912 Contact Telephone: +13026338434 USA Contact Fax: Tax ID:

Comment: RFB 10850 -- 4 year option year

Can align dates with end of warranty date.

Service Contract Quotation:

Ship To: County of San Diego Sheriffs Dept 5255 Mount Etna Dr SAN DIEGO CA 92117-6912 USA

Learn more about Agilent's Special Offers, Products, Services and our full range of laboratory productivity solutions optimized for your applications and workflows. Visit us at www.agilent.com/chem

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Page 1 of 3 Quotation Number: 5000895160 Quotation Date: April 21, 2021

Item # System Serial Model Service Description EGS Qty Start date / Total Handle Number Description End date if Price different from (USD) SC Period 1 LCMS TOF System CROSSLAB SILVER 1 163,152.00 Line Item Note: 1 G6230BA, G1953A, G7116B, G7167B, G7120A

Total Amount before Discount 163,152.00 Discount - Region/Country -42,419.52 Discount - Multi-Year PO -6,526.08 Total before Tax 114,206.40 Sales Tax 0.000% 0.00 Total (USD) 114,206.40

EGS = End of Guaranteed Support for earliest module

Summary of Charges: Note: These prices reflect additions, deletions and warranties. Year: Invoice Period: Prices by Invoice Period: YEAR 1 06/01/2022 - 05/31/2023 28,551.60

YEAR 2 06/01/2023 - 05/31/2024 28,551.60

YEAR 3 06/01/2024 - 05/31/2025 28,551.60

YEAR 4 06/01/2025 - 05/31/2026 28,551.60

Notes: Amount excludes any applicable taxes. Applicable taxes will be separately stated on the invoice at time of billing.

Exhibit URL Exhibit22X.as http://www.agilent.com/en-us/services/analytical-instrument-services/exhibit22x px Password: br8zUWuC

Page 2 of 3 Quotation Number: 5000895160 Quotation Date: April 21, 2021

This Quotation is valid only for the period indicated. All Services quoted are subject to the Agilent Service Terms (http://www.chem.agilent.com/en-US/products-services/Services/Pages/e16S_us_canada_mexico.aspx) and the Agilent Service Exhibits referenced above. Products, parts and software are subject to the Agilent Terms of Sale (http://www.chem.agilent.com/en-US/products-services/Services/Pages/e16S_us_canada_mexico.aspx). Products or Services that are manufactured, configured or adapted to meet Customer's requirements are subject to the Agilent Terms of Sales - Custom products (http://www.chem.agilent.com/en-US/products-services/Services/Pages/e16S_us_canada_mexico.aspx).If you have a previously signed agreement with Agilent covering such Products, Services or spare parts, the terms of that agreement will take precedence for those Products, Services or spare parts. Agilent expressly objects to any different or additional terms in your purchase/sales order documentation, unless agreed to in writing by Agilent. Spare parts and Service availability dates are estimated at the time of the quotation. Actual delivery dates or delivery windows will be specified at the time Agilent acknowledges and accepts your purchase order. The above conditions shall apply to the fullest extent permitted by the law governing this transaction and shall not derogate from any statutory or legal rights you may have thereunder. Commodities, technology or software exported from the United States of America ("U.S.") or from other exporting countries will be subject to the U.S. Export Administration Regulations and all exporting countries' export laws and regulations. Diversion contrary to U.S. law and the applicable export laws and regulations is prohibited. Warranty: Unless otherwise indicated herein, spare parts referenced in this document will receive a 90 day replacement warranty.

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Page 3 of 3 REQUEST FOR BID (RFB) 10850 SHERIFF’S DEPARTMENT - CRIME LAB GRAND T LIQUID CHROMATOGRAPHY SYSTEM AND MAINTEANCE SERVICES EXHIBIT C - PAYMENT SCHEDULE (Submit completed Payment Schedule with your bid)

BASE TERM (Date signed thru 06/30/22) EST. ITEM DESCRIPTION QUANTITY 1 Liquid Chromatography (LC) system - List Make and Model Number below 1 2 Freight (if applicable; no tax) Lump sum 3 Installation & Validation Lump sum 4 Training Lump sum 5 Maintenance (ongoing support) Base Year Date of Award - 06/30/22 1 6 Maintenance (ongoing support) Option Year 1 07/01/22-06/30/23 1 7 Maintenance (ongoing support) Option Year 2 07/01/23-06/30/24 1 8 Maintenance (ongoing support) Option Year 3 07/01/24-06/30/25 1 9 Maintenance (ongoing support) Option Year 4 07/01/25-06/30/26 1

Make/Model Number: 1290 Infinity II / 6230BA TOF LCMS Company Name: Manufacturer: Agilent Technologies, Inc. Name: List Warranty Duration: 2 Years Title: Lead Time: 4 Weeks Delivery ARO Phone Number: Additional info (optional): Installation and Validation are included Email: Offeror qualifies as a Preferred Vendor* (if checked, complete information below.)

*By selecting Yes and submitting a Preferred Vendor Adjusted Price, Offeror represents that it qualifies as a P San Diego Code of Administrative Ordinances and Section 3.3 of the RFB Instructions and Rules. To qualify as Business that is also a Veteran Owned Business, Disabled Veteran Owned Business or Small Business. Offeror 2. below. Offeror must provide supporting documentation upon request of the County.

1. Local Business: Offeror maintains a headquarters or provides the same or similar services to those propose located within the geographic boundaries of San Diego County. Headquarters Other location providing the same or similar services Address City State AND 2. Offeror holds a current certification that qualifies it as a Veteran Owned Business, Disabled Veteran Owne Indicate certification(s) below. State of California Certifications:

Small Business (SB) Micro Business (MB) Small Business for the Purpose of Public Works (SB-PW) Disabled Veteran Business Enterprise (DVBE) U.S. Department of Veterans Affairs Certifications: Veteran-Owned Small Business (VOSB)

Service-Disabled Veteran-Owned Small Business (SDVOSB) Preferred Vendor Adjusted Price: GRAND TOTAL PRICE ADJUSTMENT (from Pricing Schedule): multiply Box 1 by 0.05 (if greater than $50,000, enter $50,000) Box 1 ($) Box 2 ($)

County maintains the right to verify the calculation of the Adjusted Price. In the event of a mathematical erro prevail, and a corrected Price Adjustment shall be used. TOTAL: $ 378,035.10

UNIT PRICE EXTENDED PRICE $ 220,842.45 $ 220,842.45 $ - $ - $ - $ - $ 8,294.25 $ 8,294.25 $ 34,692.00 $ 34,692.00 $ 28,551.60 $ 28,551.60 $ 28,551.60 $ 28,551.60 $ 28,551.60 $ 28,551.60 $ 28,551.60 $ 28,551.60 TOTAL $ 378,035.10

Agilent Technologies, Inc. Elaine Jones Bids Response Specialist 302-636-1575 [email protected]

Preferred Vendor as described in Section 405 of the a Preferred Vendor, Offeror must be a Local must document eligibility by satisfying both 1. and

ed from the following address(es)

Zip

ed Business or Small Business.

Certificate # Certificate # Certificate # Certificate #

Certificate # Certificate #

ADJUSTED PRICE subtract Box 2 from Box 1

$ -

or, the Grand Total from the pricing schedule shall

Agilent Value Promise OUR INSTRUMENTS LAST FOR YEARS OUR COMMITMENT LASTS FOREVER AGILENT TECHNOLOGIES MAXIMIZE YOUR INSTRUMENT INVESTMENT

Plan to optimize Plan a smooth transition Through the years, you’ve trusted Agilent Technologies to provide Long before your instrument’s end-of-production date, Agilent is the instrumentation, supplies, and support needed to keep your lab helping you smoothly transition by taking full advantage of our fixed- running at peak performance. As each of your instruments reach the cost, multi-year support agreements for continuous support, service, end of its useful life and as technology applications evolve, you can and supplies, and by planning to move to the latest technology. continue looking to Agilent to maximize the longevity of your initial With our wide variety of cost-effective program options, we work investment. Let Agilent help ensure that your lab is always running to meet your specific support and system requirements throughout at its highest level of efficiency and productivity. Plan to optimize each lifecycle. During current production, you can rely on Agilent’s your assets with our timely and cost-effective transition solutions. highly-rated support to maximize your assets. When your system reaches its end-of-production date you can expect uninterrupted Promising 10 years of value support and parts, as well as a clear and strategic path to the most cost-effective transition options for optimal performance. All the The Agilent Value Promise reflects while, our 10-year Value Promise stays in force to ensure your lab upmost confidence in our unrivaled gets the most for its budget. industry standards for quality system design and manufacturing. From the date you purchase select instruments from our Agilent’s first concern is helping you maintain leading Agilent chromatography, optimal instrument uptime so your lab can spectrometry, and spectroscopy be competitively efficient and profitable. Let product lines, our Value Promise guarantees at least 10 years’ use or residual-value credit towards a replacement model upgrade. our range of service options and technology Because we stand behind our systems, our Value Promise maximizes transition solutions protect your investment. your return on investment by assuring your purchase is safe. Mike McMullen Senior Vice President & President Chemical Analysis Group

2 AGILENT TECHNOLOGIES A PROVEN PARTNER FOR YOUR SUCCESS

At Agilent, we look for ways to optimize your lab’s performance by Plan for uninterrupted lab operation enhancing our 45 years of measurement solutions expertise with constant customer communication. That is why you can rely on us to As your instrument is phased out of production, trust the Agilent provide the tools you need. Proudly, the Agilent name has become support process–from our Service Guarantee to our Value Promise. the assurance of unequalled dependability, reliability, ease of use, By informing you along the way, we keep you ahead of the curve in and enhanced productivity because we rigorously design, test and managing the lifetime of your instrument. manufacture our comprehensive array of instruments, supplies, and services to the highest standards. We become your experienced partner delivering unrivaled systems, solutions, technical support, and education to help your lab increase efficiency and profit.

AGILENT VALUE PROMISE - 10 YEARS AGILENT SERVICE GUARANTEE The Agilent Value Promise guarantees you at least 10 If you are under an Agilent Advantage contract and years of use of your new instrument, from the date of a hardware problem cannot be solved immediately, a purchase, or we will credit you with the residual value formal escalation process ensures that key technical of that system when you upgrade to a replacement and management resources quickly attend to your model1. issue. If this process does not resolve the hardware issue, we will replace your analytical instrument for free. This escalation process exemplifies how Agilent is committed to keeping your lab up and running, no matter what.

Purchase of Instrument Agilent Value Promise - 10 years of usable life

Agilent manufactures instrument 7 years after EOP

Agilent Service Guarantee - Fix or Replace

Agilent Advantage Service Contracts Asset Max Plus Asset Max

Best effort support Best effort support with parts with no parts

Product EOP EGS EOS introduction End of production End of guaranteed support End of Support

1 Applies to current generation Agilent instruments not being used in highly corrosive and bio-hazardous environments 3 Contact Us Learn more: agilent.com/chem/value

Find an Agilent Customer Center in your country: agilent.com/chem/contactus

An Agilent Service contract guarantees repair of your instrument or we replace it for free.

Information, descriptions, and specifications in this publication are subject to change without notice.

THE AGILENT COMMITMENT TO YOU

With a heritage spanning more than 75 years of invention and innovation, Agilent is committed to providing superior instruments that help you raise your performance and deepen your insight. We are dedicated to ensuring cost-effective ownership of Agilent instruments, demonstrated through the scale of our offering, the investment we put into our services, and the extent of our expertise – all of which provide you with experience you can rely on to keep your instruments operating at peak performance. A complimentary 12 month warranty for your new Agilent instrument is included with your terms of sale.

This document contains valuable information on: • Warranty overview • Welcome offers • Educational overview • What happens when my warranty expires? AGILENT CROSSLAB WELCOME PACKAGE

WARRANTY OVERVIEW

What is included? Agilent prides itself on developing the best and most reliable instruments in the industry. However, we also appreciate that the need for repairs can happen and want to ensure that should you encounter any challenges, we are able to rectify them as a priority. The warranty is in place because we are not only committed to delivering the best instruments, we are also intent on delivering the best customer service. The terms of your specific warranty can be found here; please note they are tailored per country and so it is worth familiarizing yourself with the relevant country’s warranty specification for your reference. If a product is moved to another country, the destination country’s standard warranty will apply except for on-site warranty where Agilent does not have an applicable product-specific support presence or authorized representative in that country.

When does the warranty start and what does it cover? Your complimentary warranty is valid for the first 12 months of your instrument’s life from the installation date. During this time you will be entitled to: • Preferred response time to speed resolution of your service request • All required on-site warranty-level repair visits to restore your systems to operating condition and minimize downtime • Genuine Agilent parts necessary for warranty-level repair • Dedicated technical hotline providing hardware support as often as necessary

Welcome Offers As a valued client, we at Agilent would like to take this opportunity to introduce you to some of our dedicated customer support programs. We have a range of services to ensure you get the maximum benefit from your Agilent laboratory equipment.

Am I eligible for any discounts? To extend our thanks for purchasing a new instrument from Agilent, you are also eligible to receive a 20% promotional discount on Agilent columns and supplies for your instrument. Once the shipment of your new equipment has been made you will have 90 days to register for this one-time promotional discount. Please click here for more details. Our second exclusive offer entitles you to 100 complimentary training credits to get you started on a select range of Agilent learning options. To redeem your credits, please use the key #CP100SC to register at www.agilent.com/crosslab/university. AGILENT CROSSLAB WELCOME PACKAGE

EDUCATION OVERVIEW

Improve the way you work Agilent University education provides insights that drive superior scientific, operational and economic outcomes, with a new expanded learning portfolio that matches customer location, format and time-frame requirements. We offer a wide range of training topics, resources and methods. Learn online, in the classroom, or on-site in your own lab. All courses are developed by application and technology experts, offering you the very best methods to help improve your daily workflow. Productivity gains achieved through Agilent University often result in a rapid return on investment, leading to significant long-term value.

A full portfolio of learning solutions At Agilent University we teach the latest techniques and trends in laboratory operations, maintenance, applications and workflows to help realize the full potential of the instruments in your laboratory. We tailor a wide range of learning options to the needs of your lab, helping optimize productivity on your new or existing equipment. Whether you are a lab technician, chemist, or lab manager, we offer the appropriate level of education regardless of expertise. With our continuum of learning options and courses, you can become a lifetime learner, improving your knowledge and skills throughout your career.

The right level of education when and where you need it In a classroom, in the lab, or at your desk, there are many ways to advance your knowledge around analytical methodologies and workflows. Agilent University caters to all your individual training needs by delivering a complete curriculum of learning solutions in the location, format and time-frame you require. • Formal classroom training at an Agilent site • Customized training at your site • Online, self-paced learning through eLearning modules and recorded lectures • Virtual Instructor-Led Training • Application consulting in your laboratory For more information on Agilent training near you, please visit the website. AGILENT CROSSLAB WELCOME PACKAGE

WHAT HAPPENS WHEN MY WARRANTY EXPIRES?

Your complimentary warranty provides a complete support package for your new equipment for the first 12 months following installation. To extend your warranty, once it has expired, the reassurance of an Agilent CrossLab service agreement will support you through the remaining lifetime of your instrument. Agilent CrossLab delivers timely, tailored solutions that improve your science and enhance your laboratory operations – from a single instrument all the way through your entire workflow. Thanks to our 360o perspective, we synchronize our services to create a complete solutions package that addresses your challenges and provides immediate, lasting benefits for all of your laboratory processes.

AGILENT VALUE PROMISE - 10 YEARS AGILENT SERVICE GUARANTEE The Agilent Value Promise guarantees you at least 10 If you are under an Agilent CrossLab contract and a years of use of your new instrument, from the date of hardware problem cannot be solved immediately, a purchase, or we will credit you with the residual value formal escalation process ensures that key technical of that system when you upgrade to a replacement and management resources quickly attend to your model1. issue. If this process does not resolve the hardware issue, we will replace* your analytical instrument for free. This escalation process exemplifies how Agilent is committed to keeping your lab up and running, no matter what.

1 Applies to current generation Agilent instruments not being used in highly * Free replacement of your analytical instrument is not valid for Agilent corrosive and bio-hazardous environments. CrossLab Extended Services.

Purchase of Instrument Agilent Value Promise - 10 years of usable life

Agilent manufactures instrument 7 years after EOP

Agilent Service Guarantee - Fix or Replace

Agilent CrossLab Service Contracts Agilent CrossLab Extended Services

Product EOP EGS EOS introduction End of production End of guaranteed support End of Support AGILENT CROSSLAB WELCOME PACKAGE

MULTI-VENDOR SERVICE AND SUPPORT

Agilent offers expert maintenance and repair of instruments from many manufacturers. Whatever your laboratory configuration, Agilent CrossLab can combine onsite service and remote diagnostics to deliver the exact level of support that best meets your goals and budget. Choose from a range of service options that maximize throughput, ensure compliance, and minimize costly downtime.

Agilent CrossLab Service Contract

PREVENTIVE GOLD SILVER BRONZE MAINTENANCE Services Included in All Agilent CrossLab Service Agreements

Contract-level preferred response vs. T&M ★ ★ ★

Hardware telephone support ★ ★ ★

Software telephone support ★ ★ ★

Onsite Repair Services

Unlimited onsite repair visits (travel & labor) ★ ★ ★

Parts required for repair ★ ★ ★

Consumables/supplies required for repair,* including ★ ★ ★ liners, seals, tubing, assemblies, and multipliers

Maintenance Services

Annual onsite preventive maintenance ★ ★ ★

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Agilent remote advisor-assist** ★ ★ ★

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Extended coverage hours discount ★

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Discount when bundling operational qualification (OQ) ★ ★

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Enterprise Asset Management A comprehensive approach to asset lifecycle management that helps define the precise instruments for your needs and then enhances their utility and lifecycle value to achieve your desired business outcomes.

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This information is subject to change without notice.

© Agilent Technologies, Inc. 2016 Printed in the USA September 24, 2015 5991-5993ENUC Agilent Fact Sheet Revenue Mix by End Market At a Glance: Chemical Pharma President and CEO: Mike McMullen & Energy 33% Headquarters: Santa Clara, California 21% Number of Employees: Approximately 16,300 NYSE: A Food Academia & 10% Government Total Market Profile: 10%

Global Leader in Life Sciences, Environmental Diagnostics Diagnostics and Applied Markets & Forensics & Clinical 11% 15% • Market size1: $58 billion Revenue by Geography Key Platforms: • Liquid and gas chromatography • Mass spectrometry Americas Europe 37% 27% • Spectroscopy • Genomics and diagnostics • Consumables, services and software

Growth Drivers: Asia Pacific • Food and water quality 36% • Rising energy demands Headcount by Geography • Healthy and aging population • Emerging economies

Americas Europe 25% FY20 Financial Stats: 37% • Revenues: U.S. $5.3 billion + 1% core growth3,4 • Adjusted operating margin2: 23.5%4 of revenue

Asia Pacific 38%

1. Total Available Market. Market size per Company estimates. 2. FY20 Revenue January 2021 3. Core growth is reported growth adjusted for the effects of acquisitions and FX. 4. Presented on a non-GAAP basis, reconciliations to closest GAAP equivalent provided.

Why Stop at Detection? Start Identifying

Agilent 6230 time-of-flight LC/MS system

It’s Time for More Specific Detection It’s Time for Time-of-Flight LC/MS

Screening samples by HPLC enables rapid compound detection. With UV and quadrupole MS detection, great insights on mixture compositions can be achieved. When the need arises for even more selectivity, accessible accurate mass measurement delivers enhanced identification confidence.

Your screening workflows don’t end with signal detection. With Agilent time-of-flight LC/MS, neither does your analytical platform. The Agilent 6230 time-of-flight LC/MS system provides accurate mass measurement with high mass resolution for confident compound identification beyond conventional HPLC detection. Reveal the full picture of the compounds present in your samples with the Agilent 6230 LC/TOF.

x10 2 DAD1 - dad1A:dad1A SB_CN.d 3.5

3.25

2.75 2.5 Peak 1 2.25 UV, nominal MS detection,

2 0.94 Peak 2 526.59 1.75 1.06 and other analytical techniques 516.79 1.5

1.25 might alert you to only the 1 presence, class or nominal mass 0.75 0.5 of a compound. 0.25

-0.25 x10 5 Peak 1 0.7 0.8 0.9 1 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 2.9 3 3.1 3.2 1.6 TOF MS Spectrum Response Units vs. Acquisition Time (min) 1.5 171.0290 1.4 1.3 1.2 1.1 1 0.9 0.8 193.0110 0.7 0.6 0.5 0.4 0.3 0.2 0.1 188.0556 208.9761 214.9932 221.0287 0 158 160 162 164 166 168 170 172 174 176 178 180 182 184 186 188 190 192 194 196 198 200 202 204 206 208 210 212 214 216 218 220 222 Counts (%) vs. Mass-to-Charge (m/z) x105 5 Peak 2 4.5 TOF MS Spectrum 127.0391 4 LC/TOF goes one step further: 3.5 It provides accurate mass 3 information to help you identify 2.5

2 the compounds you detect.

1.5

1 149.0213 0.5 128.0423 111.0475 116.9819 118.9507 125.0223 130.1530 133.0882 136.1085 142.0866 144.0682 145.9658 150.0254 0 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154

Counts (%) vs. Mass-to-Charge (m/z)

3 Now Anyone Can Achieve Reliable Results—Regardless of Skill Level

A comparison between classic and SWARM tuning Producing expert-level results shouldn’t require expert-level training. results for the low mass metabolite malate. With the Agilent 6230 LC/TOF system, it won’t. 106 -ESI EIC(133.0137) Scan Frag130.0V 20180710SWARMTunemz1700 LCIP2FinalMix3.d S –– Experience the best possible mass spectrometer performance with a 2.5 2.4 single click through innovative, user-friendly SWARM autotune. 2.3 O 2.2 2.1 HO –– Give everyone in your lab easy access to compound identification with 2 OH MassHunter Walkup software. 1.9 1.8 O OH 1.7 –– Go beyond UV or quadrupole MS detection with full-spectrum, 1.6 Malate 134.09 g/mol high-resolution TOF data. 1.5 m/z 133.01373 1.4 1.3 1.2 1.1 Agilent SWARM autotune quickly searches across a wide parameter space, 1 0.9 converging on the optimal settings to ensure your best results. 0.8 SWARM Results 0.7 Classic Results 0.6 0.5 0.0016700M 0.4 0.3 0.0016695M 0.2 0.1 0 0.0016690M 7.25 7.3 7.35 7.4 7.45 7.5 7.55 7.6 7.65 Counts () vs. Acquisition Time (min) 0.0016685M

0.0016680M

0.0016675M Minor Md

0.0016670M

0.0016665M

0.0016660M

0.0016655M

0.0016650M 43.0 43.2 43.4 43.6 43.8 44.0 44.2 44.4 44.6 44.8 45.0 Bot Sit Multidimensional Tuning TOF Mirror and Slit Correct Best Solution is (44.00 1,667.65)

Particle 0 Particle 1 Particle 2 Particle 3 Particle 4 Particle 5 Particle 6 Particle 7 Particle 8 Particle 9

4 Managing your LC/MS applications with MassHunter Walkup software increases efficiency, consistency, and throughput. You’ll also minimize errors associated with maintaining multiple diverse workflows.

Compound verification Intact protein analysis Peptide mapping and purity assessment

Sample preparation Enzymatic digestion Sample preparation

Upload expected formula Upload sequence Sample preparation or molecular weight

MS spectrum Upload sequence Chromatographic deconvolution separation

Data acquisition Zero-charge mass Peak detection determination

Compound extraction

Sequence matching Assess peak MS spectra

Sequence matching

Determine post-translational Compare expected monoisotopic mass modifications Determine posttranslational to observed m/z considering adducts modifications Optional high-throughput compound Optional ADC DAR calculation purity assessment with Sequence coverage Analytical Studio Reviewer

Reporting

Delivery of results

5 Confidence Beyond UV Detection

The All Ions approach enables easier method setup, more sophisticated data processing, and—ultimately—higher confidence in target compound identifications.

–– Reduce identification uncertainty with exacting isotopic fidelity and high-resolution, accurate-mass spectra. –– Improve compound selectivity. Data-independent acquisition enables spectral library matching of precursor and product ion spectra. –– Detect analytes down to trace levels with available Agilent Jet Stream technology.

Number of theoretical formulas for a compound composed of C, H, N, and O with a molecular weight of 609.28066 m/z

Number of Mass Accuracy (mDa) Mass Accuracy (ppm) MS Technique Matching Formulas

0.1005 165 209 Nominal mass (e.g., quadrupole)

0.0061 10 13 ...

0.0030 5 7 ...

0.0018 3 4 ...

0.0012 2 2 Accurate mass (e.g., 6230 LC/TOF)

Cpd1 C33 H40 N2 O9 ESI Scan (rt 0.242-0.399 min,15 scans) ReserpineTOF100pgms3.d x105 1.3 609.2806 1.2 (C33H40N2O9H) 1.1 1 0.9 0.8 0.7 0.6 610.2836 (C33H40N2O9H) 0.5 Reserpine m/z 609.28066 0.4 0.3 611.2865 0.2 (C33H40N2O9H) 612.2892 613.2974 0.1 (C33H40N2O9H) (C33H40N2O9H) 0 609 609.5 610 610.5 611 611.5 612 612.5 613 613.5 614 Counts () vs. Mass-to-Charge (m/z)

Isotopic matching of reserpine (C33H40N2O9). The red boxes show theoretical isotope abundance and position.

6 Qualitative Analysis software enables confident compound identification by comparing measured mass accuracy, chromatographic co-elution, and retention time to high-resolution spectral libraries with precursor and product ion spectra.

LC sample inlet

Enhance LC/MS sensitivity 5-fold Agilent Jet Stream thermal focusing Heated drying gas technology significantly increases LC/MS sensitivity by improving the spatial focusing of electrospray Superheated MS inlet droplets. The subsequent ion density sheath gas and desolvation enhancements result in higher MS signal intensities and Nozzle voltage improved signal-to-noise ratios. Heated drying gas Collimated thermal containment zone results in enhanced MS sensitivity Resistive sampling capillary

7 Tailor Your System to Both Large and Small Molecules

With the Agilent 6230 LC/TOF system, you can identify both small molecules and large biological compounds with high-resolution, accurate-mass data. –– Confidently analyze intact proteins and other large molecules with high TOF vacuum levels and a wide mass range using MassHunter BioConfirm software. –– Optimize small-molecule sensitivity by choosing from a wide range of ion sources. –– Detect trace-level compounds in the presence of coelution with a wide in-spectrum dynamic range.

These three spectra show a progressive analysis of x102 ESI Scan (rt 2.551 - 2.600 min, 4 scans) Frag400.0V Intact NIST mAb500ng01.d 5.5 intact NIST mAb. The high TOF vacuum level efficiently 3025.423 5.25 5 transmits large molecules that are beyond the 4.75 practical range of other mass spectrometer platforms. 4.5 3088.461 4.25 2745.407 The magnified spectrum shows excellent separation 4 3154.156 3.75 between proteoforms, with the deconvoluted spectrum 3.5 3.25 retaining this enhanced selectivity. 3 2.75 2512.822 2.5 3222.657 2.25 2 1.75 3294.283 1.5 2353.361 1.25 1 0.75 0.5 0.25 0

2100 2200 2300 2400 2500 2600 2700 2800 2900 3000 3100 3200 3300 3400 3500 3600 3700 x102 ESI Scan (rt 2.551 - 2.600 min, 4 scans) Frag400.0V Intact NIST mAb500ng01.d 5.75 Counts () vs. Mass-to-Charge (m/z) 5.5 5.25 2906.816 5 2909.95 4.75 4.5 4.25 4 3.75 3.5 3.25 2903.598 3 2.75 2913.165 2.5 2.25 x102 ESI Scan (rt 2.534 - 2.683 min, 10 scans) Frag400.0V Intact NIST mAb500ng01.d 2 1.25 Subtract econvoluted (Isotope Width0.0) 1.75 1.225 1.5 2915.990 1.2 1.25 1.175 1.15 10F, 11F (1607.5013) 1 1.125 0.75 1.1 18198.70 1.075 0.5 1.05 0.25 1.025 0 1 0.975 2890 2892 2894 2896 2898 2900 2902 2904 2906 2908 2910 2912 2914 2916 2918 2920 2922 2924 2926 2928 2930 0.95 0.925 0.9 21F (1607.5013) Counts () vs. Mass-to-Charge (m/z) 0.875 18360.90 0.85 0.825 0.8 0.775 0.75 0.725 0.7 mAb 0.675 20F (NA2F)(15.3580) 0.65 18036.8 0.625 0.6 0.575 0.55 0.525 11F,12F (NA2F)(1769.65) 0.5 18521.35 0.475 0.45 0.425 0.4 0.375 0.35 0.325 0.3 22F (NA2F)(1769.65) 0.275 0.25 1868.32 0.225 0.2 0.175 11F-lcNAc (1386.2899) 0.15 17832.90 188.15 0.125 0.1 17969.59 18997.73 0.075 0.05 17628.71 18767.87 1911.77 0.025 0 147400 147500 147600 147700 147800 147900 148000 148100 148200 148300 148400 148500 148600 148700 148800 148900 149000 149100 149200 149300 Counts () vs. econvoluted Mass (amu)

8 Detect the Unexpected—Even at Trace Levels

With a variety of available ion sources to analyze compounds of differing polarities and weights, the 6230 LC/TOF offers the full-spectrum sensitivity needed for challenging detection limits. A broad in-spectrum dynamic range means trace-level analyte molecules and their isotopes are accurately detected even in the presence of abundant coelution.

x105

1.4 346.1006

1.2

1 191.0196 0.8 286.0793 0.6

0.4 521.9827 0.2 983.0347 805.9861 633.1838 1099.9794 0 100 200 300 400 500 600 700 800 900 1000 1100 1200 1300 Counts () vs. Mass-to-Charge (m/z)

x10 2 521.9835 1.2 (C10H16N5O14P3-H)- 1.1 0.19 PPM 1 0.9 0.8 0.7 0.6 Guanosine triphosphate 0.5 0.4 522.9861 0.3 (C10H16N5O14P3-H)- 523.9880 0.2 0.38 PPM (C10H16N5O14P3-H)- 0.38 PPM 0.1 0 521.6 521.8 522 522.2 522.4 522.6 522.8 523 523.2 523.4 523.6 523.8 524 524.2 524.4 524.6 524.8 525 525.2 Counts () vs. Mass-to-Charge (m/z)

9 Future-Proof Your Analyses

As with any Agilent instrument, you can expect consistent, reliable data over the lifetime of the 6230 LC/TOF system. –– Enable retrospective analysis—without reinjection—through full-time, full-spectrum acquisition. –– Prepare for evolving analytical requirements with a capable, flexible platform. –– Combine world-class detection and separation with leading Agilent chromatographic systems, including HPLC/UHPLC, SFC, CE, and GC. –– Protect your investment with a guaranteed 10 years of useable instrument life—including 7 years of guaranteed support.

Review

Full-spectrum data

Analyze Acquire

Inject once and reanalyze as needed with full-spectrum data for complete retrospective analysis. The Agilent 6230 LC/TOF system lets you refer back to your data anytime, without rerunning, to investigate samples further.

10 Agilent InfinityLab LC Series Systems: The Perfect Partners for the Agilent 6230 LC/TOF System

1220 Infinity II LC 1260 Infinity II LC 1290 Infinity II LC Affordable efficiency Everyday efficiency The benchmark in efficiency This high-quality, integrated A trusted platform with the broadest This next generation of liquid system puts you on the fast choice of instrumentation, this system chromatography instrumentation track to efficiency—for maximum gives you the best operational efficiency sets the standard for ultrahigh return on investment. for maximum confidence, day after day. performance and efficiency.

Simplified LC/MS for everyone Enable routine protein characterization With Agilent MassHunter Walkup software, users of Using accurate-mass LC/MS or LC/MS/MS data, Agilent different skill levels can perform their own automated MassHunter BioConfirm software helps you confirm LC and LC/MS analyses. They simply input basic identities and identify variants before you start expensive information, choose a method, and insert samples as testing. It also features a drug-to-antibody ratio calculator directed. What’s more, results automatically appear in for characterizing biopharmaceutical products, such as the user’s inbox. monoclonal antibodies.

11 Learn more: www.agilent.com/chem/6230b Buy online: www.agilent.com/chem/store U.S. and Canada 1-800-227-9770 [email protected] Europe [email protected] Asia Paciﬁc [email protected]

This information is subject to change without notice.

The Agilent 1260 Infinity II Flexible Pump

The Benchmark for High Accuracy and Precision in Low Pressure Mixing Gradient Pumps

800

700

600

500 U

mA 400

300

200

100

0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.56.0 6.57.0 7.58.0 Time (min)

Authors Abstract Melanie Metzlaff and The Agilent 1260 Infinity II Flexible Pump provides the highest flexibility for running Patric Hörth conventional applications on 4.6 mm columns or fast runs on 2.1 and 3.0 mm id Agilent Technologies, Inc. columns with no compromise regarding precision and accuracy. This Technical Overview demonstrates the outstanding performance of the 1260 Infinity II Flexible Pump. Precision of retention times under demanding conditions was evaluated and compared to the Agilent 1260 Infinity II Binary Pump. In addition, the composition accuracy, delay volume, and flow accuracy of both pumps were evaluated by running step gradients. Introduction Merck (Darmstadt, Germany). Trifluoroacetic acid (TFA) and formic acid The Agilent 1260 Infinity II Flexible Pump (FA) were purchased from Sigma-Aldrich is a low-pressure mixing gradient pump, (Steinheim, Germany). and is capable of 800 bar operating Methods pressure. The integrated multipurpose valve enables convenient features Table 1. Chromatographic conditions for step gradients. such as automatic purge, switching an Parameter Value additional mixer in and out, and filter Column Restriction capillary backflush. A) 0.3 % Acetone in water Solvent To demonstrate the superior B) Water Between 20 and 45 minutes in 1 % steps from 100 to 94 %A performance of the 1260 Infinity II Between 45 and 55 minutes in 2 % steps from 94 to 90 %A Between 55 and 80 minutes in 10 % steps from 90 to 51 %A Flexible Pump, different demanding Gradient Between 80 and 90 minutes in 1 % steps from 51 to 49 %A applications were chosen. Results Between 90.01 and 110 minutes in 10 % steps from 40 to 10 % obtained with the 1260 Infinity II Flexible Between 110.01 and 135 minutes in 2 % steps from 6 to 0 %A Pump were compared to the results Stop time 140 minutes obtained with the Agilent 1260 Infinity II Flow rate 1 mL/min Binary Pump. Column temperature 30 °C DAD 273/4 nm, Ref. 360/100 nm, 10 Hz

Experimental Table 2. Chromatographic conditions for shallow gradient runs.

Instrumentation Parameter Value Polyphenolic mix: Catechin, caffeic acid, epicatechin, epigallocatechin Compounds For the experiments, the following (all purchased from Sigma-Aldrich) modules were used: Column Agilent ZORBAX StableBond C18, 2.1 × 50 mm, 1.8 µm (p/n 857700-902) A) 0.1 % FA in water • Agilent 1260 Infinity II Flexible Pump Solvent B) 0.1 % FA in acetonitrile (G7104C) 0 minutes–6 %B Gradient • Agilent 1260 Infinity II Binary Pump 8 minutes–12 %B (G7112B) Stop time 8 minutes Post time 3 minutes • Agilent 1260 Infinity II Multisampler Flow rate 0.6 mL/min (G7167A), equipped with an Injection volume 2 µL with 3 seconds needle wash (60 % acetonitrile in water) integrated sample cooler Sample temperature 10 °C (option #100) Column temperature 30 °C • Agilent 1260 Infinity II MCT DAD 280/4 nm, Ref. 360/50 nm, 40 Hz (G7116A) Table 3. Chromatographic conditions for low organic percentage runs. • Agilent 1260 Infinity II Diode Array Detector WR (G7115A), equipped Parameter Value Sulfa drugs: Sulfanilamid, sulfathiazole, sulfamerazine, with a standard flow cell: 10 mm Compounds sulfachloropyridazine, sulfamethazine (all purchased from Sigma-Aldrich) path length,13 µL volume Column Agilent ZORBAX StableBond C18, 2.1 × 50 mm, 1.8 µm (p/n 857700-902) A) 0.1 % TFA in water Software Solvent B) 0.1 % TFA in acetonitrile Agilent OpenLAB CDS 2.2 (M8413A) 0 minutes–1 %B Gradient 6 minutes–25 %B Chemicals Stop time 8 minutes All solvents were LC grade. Fresh Post time 3 minutes ultrapure water was obtained from a Flow rate 0.6 mL/min Milli-Q Integral system equipped with Injection volume 2 µL with 3 seconds needle wash (60 % acetonitrile in water) a 0.22 µm membrane point‑of‑use Sample temperature 10 °C cartridge (Millipak). Acetonitrile Column temperature 45 °C and acetone were purchased from DAD 254/4 nm, Ref. 360/80 nm, 20 Hz

2 Results and Discussion Table 4. Chromatographic conditions for high organic percentage runs. Parameter Value Experiment 1: Step gradient Sudan red mix: Sudan I, Sudan II, Sudan III, Sudan IV (all purchased from Compounds Step gradients, including a tracer in Sigma-Aldrich) solvent A, were used to determine the Column Agilent ZORBAX Eclipse Plus C18, 3.0 × 50 mm, 1.8 µm (p/n 959941-302) A) Water Solvent system delay volume, the composition B) 95 % Acetonitrile in water accuracy, mixing noise, and flow 0 minutes–90 %B Gradient accuracy. For the flow accuracy 4 minutes – 100 %B evaluation, a flow accuracy meter (FAM) Stop time 4 minutes was used. Post time 2 minutes Flow rate 1 mL/min Step gradients under identical conditions Injection volume 1 µL with 3 seconds needle wash (acetonitrile) were applied to the 1260 Infinity II Sample temperature 10 °C Flexible Pump, with and without the Column temperature 40 °C Jet Weaver mixer, and to the 1260 DAD 490/20 nm, no Ref., 20 Hz Infinity II Binary Pump. Figure 1 shows an overlay of the three step gradients Table 5. Chromatographic conditions for fast gradient runs. running from 100 %A (water + tracer) Parameter Value to 0 %A (water + tracer) in 1, 2, and Compounds Agilent RRLC checkout sample (p/n 5188-6529) 10 % steps. Both pumps showed Column Agilent ZORBAX Eclipse Plus C18, 3.0 × 50 mm, 1.8 µm (p/n 959941-302) excellent performance over the complete A) Water Solvent gradient range. The gradient steps were B) Acetonitrile perfectly resolved even for the 1 % steps 0 minutes–30 %B Gradient at low % solvent B. These results clearly 1 minutes–100 %B demonstrate an equivalent gradient Stop time 2 minutes performance of the 1260 Infinity II Post time 1 minutes Flexible Pump compared to the Flow rate 2 mL/min 1260 Infinity II Binary Pump, even for Injection volume 1 µL with 3 seconds needle wash (60 % acetonitrile in water) demanding ranges like 1 to 5 %B. Sample temperature 10 °C Column temperature 60 °C DAD 254/4 nm, Ref. 360/60, 80 Hz

Norm. Agilent 1260 Infinity II Flexible Pump with the 380 µL Jet Weaver Mixer Agilent 1260 Infinity II Flexible Pump without the mixer 600 Agilent 1260 Infinity II Binary Pump

500

400

Norm. 300 585

200 580 575 100 570

565 0 20 25 30 35 40 45 min

-100 20 40 60 80 100 120 min

Figure 1. Step gradients from 100 to 0 % tracer on an Agilent 1260 Infinity II Flexible Pump with and without a mixer, and on an Agilent 1260 Infinity II Binary Pump.

3 Composition accuracy was calculated for a more stable UV signal due to a better For the 1260 Infinity II Flexible Pump, both pumps based on the step gradients. mixing performance. These results the real flow rate (0.998 mL/min) was Figure 2 shows the composition clearly demonstrate the outstanding slightly lower than the programmed accuracy for the 1260 Infinity II Flexible performance of the Jet Weaver mixer flow rate of 1 mL/min. The real flow Pump (with and without the 380 µL Jet with efficient mixing and smart design rate for the 1260 Infinity II Binary Pump Weaver mixer) and for the 1260 Infinity II to keep the delay volume as low as showed slightly higher values with Binary Pump. The specifications for possible. 1.003 mL/min. However, both pumps composition accuracy were defined as By adding a FAM to the LC stack performed excellently, and were inside ±0.35 % for the 1260 Infinity II Binary configuration, the actual flow over the the specification range of ±1 %. Pump and ±0.4 % for the 1260 Infinity II step gradient runs was monitored. Flexible Pump. The composition accuracy for both pumps was inside the specified range. 1.0 Agilent 1260 Infinity II Flexible Pump with a Agilent 1260 Infinity II Agilent 1260 Infinity II Table 7 summarizes the system delay 0.8 380 µL Jet Weaver mixer Flexible Pump without a mixer Binary Pump volume, mixing noise, and flow accuracy. As expected, the 1260 Infinity II Flexible 0.6 Pump without the Jet Weaver mixer had 0.4 +0.35 % the lowest delay volume, approximately cy (%) 0.2 310 µL. Switching the 380 µL Jet Weaver mixer into the flowpath increased the 0 delay volume to only 460 μL. For the -0.2 1260 Infinity II Binary Pump, a delay -0.35 % -0.4 volume of 610 µL was calculated. All Composition accu ra pump delay volumes include the volume -0.6 of the capillaries, restriction capillary, -0.8 and detector cell. The 1260 Infinity II -1.0 Multisampler was not part of the 0 20 40 60 80 100 0 20 40 60 80 100 0 20 40 60 80 100 flowpath for these measurements. Setpoint solvent B (%)

The mixing noise was calculated for Figure 2. Composition accuracy for the Agilent 1260 Infinity II Flexible Pump with and without a mixer, and every step over the complete gradient. for the Agilent 1260 Infinity II Binary Pump. Table 7 compares the values at three different steps: 95 %, 49 %, and 4 % Table 7. Step gradients results. tracer. The 1260 Infinity II Flexible Pump Agilent 1260 Infinity II and the 1260 Infinity II Binary Pump Flexible Pump with the 380 µL Agilent 1260 Infinity II Agilent 1260 Infinity II showed similar results. However, the Jet Weaver mixer Flexible Pump Binary Pump 1260 Infinity II Flexible Pump with the Delay volume ~460 µL ~310 µL ~610 µL 380 µL Jet Weaver mixer provided the Mixing noise at 95 %A 0.015 % 0.048 % 0.023 % best results regarding the mixing noise. Mixing noise at 49 %A 0.024 % 0.040 % 0.045 % With the additional mixer, the delay Mixing noise at 4 %A 0.013 % 0.034 % 0.012 % volume of the 1260 Infinity II Flexible Mean flow rate (mL/min) 0.998 0.998 1.003 Pump increased slightly, but facilitates Mean ∆ flow rate (%) -0.164 -0.155 0.270

4 Experiment 2: Retention time precision for shallow gradients To determine the retention time precision under shallow gradient conditions, a mix of four polyphenolic compounds was analyzed. The gradient went from 6 %B Agilent 1260 Infinity II to 12 %B in eight minutes, representing Flexible Pump a change of solvent B of 0.75 % per U minute. Figure 3 shows an overlay of two mA chromatograms, one acquired with the 1260 Infinity II Flexible Pump (without additional mixer) and the other with the 1260 Infinity II Binary Pump. The Agilent 1260 Infinity II retention time precisions (Table 8) for Binary Pump the four polyphenolic compounds were Time (min) similar on both pumps. This experiment demonstrates the high mixing precision Figure 3. Analysis of polyphenolic compounds using a shallow gradient. Elution order: catechin, of the 1260 Infinity II Flexible Pump even caffeic acid, epicatechin, then epigallocatechin. under shallow gradient conditions, where a binary pump is preferred. Table 8. Retention time precision for the analysis of polyphenolic compounds. Eight consecutive runs were used for the calculation. Experiment 3: Retention time Agilent 1260 Infinity Flexible Pump precision for low organic percentage Catechin Caffeic acid Epicatechin Epigallocatechin A mix of five sulfa drugs was analyzed, Average RT (min) 2.019 2.372 3.979 4.426 running a gradient from 1 to 25 % Standard deviation RT 0.0018 0.0015 0.0042 0.0036 organic (Figure 4). For this low organic RSD RT 0.087 0.061 0.106 0.081 percentage range, a binary pump is Agilent 1260 Infinity II Binary Pump advantageous. The 1260 Infinity II Average RT (min) 2.348 2.710 4.927 5.491 Flexible Pump is specified for a Standard deviation RT 0.0035 0.0023 0.0032 0.0033 composition range of 0 to 100 %. To RSD RT 0.151 0.085 0.066 0.060 evaluate the performance at this low organic percentage range for both pumps, the retention time precision was calculated from eight consecutive runs (Table 9). Both pumps showed excellent performance regarding retention time precision. For the two early eluting compounds sulfanilamide Agilent 1260 Infinity II Flexible Pump and sulfathiazole, the 1260 Infinity II mAU Flexible Pump even outperformed the 1260 Infinity II Binary Pump.

Agilent 1260 Infinity II Binary Pump

Time (min)

Figure 4. Analysis of sulfa drugs at low organic percentage. Elution order: sulfanilamide, sulfathiazole, sulfamerazine, sulfachloropyridazine, then sulfamethazine.

5 Experiment 4: Retention time Table 9. Retention time precision for the analysis of sulfa drugs. precision for high organic percentage Agilent 1260 Infinity Flexible Pump For this experiment, Sudan Red 1, 2, 3, Sulfanilamid Sulfathiazole Sulfamerazine Sulfachloropyridazine Sulfamethazine and 4 were analyzed using a gradient Average RT (min) 0.505 2.926 3.607 3.935 4.586 from 90 to 100 % organic (Figure 5 and Standard 0.0005 0.0009 0.0012 0.0013 0.0005 Table 10). The results show that the deviation RT 1260 Infinity II Flexible Pump is well RSD RT 0.092 0.032 0.033 0.033 0.010 suited for this kind of application, and Agilent 1260 Infinity II Binary Pump performs better than the 1260 Infinity II Average RT (min) 0.499 3.468 4.331 4.671 5.355 Binary Pump. Standard 0.0009 0.0044 0.0022 0.0022 0.0024 deviation RT RSD RT 0.174 0.127 0.050 0.047 0.045

Agilent 1260 Infinity II Flexible Pump mAU

Agilent 1260 Infinity II Binary Pump

Time (min)

Figure 5. Analysis of a mix of Sudan Red compounds at high organic percentage. Elution order: Sudan Red 1, Sudan Red 2, Sudan Red 3, then Sudan Red 4.

Table 10. Retention time precision for the analysis of Sudan Red compounds. Eight consecutive runs were used for the calculation.

Agilent 1260 Infinity Flexible Pump Sudan I Sudan II Sudan III Sudan IV Average RT (min) 0.643 1.085 1.497 2.656 Standard deviation RT 0.0004 0.0008 0.0007 0.0014 RSD RT 0.055 0.070 0.047 0.053 Agilent 1260 Infinity II Binary Pump Average RT (min) 0.630 1.058 1.468 2.640 Standard deviation RT 0.0009 0.0018 0.0027 0.0044 RSD RT 0.147 0.173 0.187 0.166

6 Experiment 5: Retention time precision for fast runs To enable shorter runs, a high flow rate Agilent 1260 Infinity II Binary Pump of 2 mL/min was applied. The gradient low delay configuration time was set to one minute, and went from 30 to 100 % organic (Figure 6). Agilent 1260 Infinity II Binary Pump For the 1260 Infinity II Binary Pump, standard configuration the standard configuration and the low delay configuration were tested. mAU

For the low delay configuration, the Agilent 1260 Infinity II Flexible Pump damper and mixer were bypassed. The standard configuration 1260 Infinity II Flexible Pump was used with and without the mixer. The pressure Agilent 1260 Infinity II Flexible Pump for all system configurations was with the 380 µL Jet Weaver mixer approximately 540 bar. The 1260 Infinity II Binary Pump with Time (min) the low delay configuration provided Figure 6. Analysis of the RRLC checkout sample using a high flow rate. the shortest run time, followed by the 1260 Infinity II Flexible with the standard configuration. Table 11. Retention time precision for the analysis of the RRLC checkout sample. Three representative peaks were chosen. Eight consecutive runs were used for the calculation. To demonstrate the retention time precision for these fast chromatographic Agilent 1260 Infinity II Flexible Pump—standard configuration runs (Table 11), three representative Peak 3 Peak 6 Peak 8 0.697 0.977 1.166 peaks were chosen. Even under these Average Standard deviation 0.0004 0.0005 0.0005 demanding conditions, the retention RSD 0.051 0.047 0.040 time precision for both pumps and their Agilent 1260 Infinity II Flexible Pump with 380 µL Jet Weaver mixer corresponding configuration showed Average 0.766 1.135 1.341 excellent values, with <0.09 %RSD. Standard deviation 0.0005 0.0004 0.0000 In an additional experiment, the flow RSD 0.060 0.031 0.003 rate was increased from 2 mL/min to Agilent 1260 Infinity II Binary Pump—standard configuration 2.6 mL/min, and the resulting system Average 0.813 1.112 1.290 pressure was approximately 750 bar. For Standard deviation 0.0005 0.0005 0.0006 this experiment, only the 1260 Infinity II RSD 0.066 0.048 0.050 Flexible Pump in standard configuration Agilent 1260 Infinity II Binary Pump low delay configuration was used, given that this pump has Average 0.614 0.850 1.023 an operating pressure range of up to Standard deviation 0.0005 0.0006 0.0005 800 bar, whereas the 1260 Infinity II RSD 0.084 0.074 0.047 Binary Pump is limited to 600 bar.

7 By increasing the flow rate to Compound Average RT (min) RSD RT (%) 2.6 mL/min, the gradient run time 1. Acetanilide 0.171 0.103 was reduced from 1 to 0.77 minutes 2. Acetophenone 0.351 0.071 (Figure 7). All peaks eluted below 3. Propiophenone 0.546 0.037 one minute, with an outstanding 4. Butyrophenone 0.668 0.021 precision of retention times, below the 5. Valerophenone 0.710 0.019 specification of 0.15 %RSD. The last 6. Hexanophenone 0.763 0.025 compound eluted earlier (0.967 minutes) 7. Heptanophenone 0.842 0.025 with the 1260 Infinity II Flexible Pump 8. Octanophenone 0.909 0.024 than with the 1260 Infinity II Binary 9. Benzophenone 0.967 0.024 Pump (1.095 minutes) with a flow rate of ×102 2 mL/min in low delay configuration. 5.0 5 4.5 Conclusion 4.0 3.5 1 U 3.0 2 The Agilent 1260 Infinity II Flexible mA 2.5 3 4 2.0 6 7 Pump provided excellent performance 8 1.5 9 regarding retention time precision for 1.0 various challenging LC applications. The 0.5 0 applied step gradients showed excellent 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.80.9 1.01.1 1.21.3 1.41.5 composition accuracy and mixing noise Time (min) for the 1260 Infinity II Flexible Pump and Figure 7. Overlay of eight chromatograms applying a flow rate of 2.6 mL/min at 750 bar backpressure. the Agilent 1260 Infinity II Binary Pump. Real-time flow rates were measured and monitored for both pumps, showing that the flow accuracy was always below 0.3 %. The system delay volume of the 1260 Infinity II Flexible Pump is approximately 310 µL, and 460 μL with the 380 µL Jet Weaver mixer. For the 1260 Infinity II Binary Pump, the system delay volume adds up to 610 µL. This Technical Overview shows that both pumps demonstrated excellent performance. In addition, the 1260 Infinity II Flexible Pump showed excellent results for demanding applications where a binary pump is normally advantageous.

www.agilent.com/chem

This information is subject to change without notice.

Data Sheet

The Agilent 6230 Accurate-Mass TOF LC/MS achieves sensitivity, mass accuracy, mass resolution, and scan speed specifications after autotune without manual user intervention.

Parameter Measure Specification MS sensitivity with AJS 1 pg reserpine injected on-column Signal-to-noise ratio > 50:1 Measured at m/z 609.2807 Noise = 1 × RMS MS sensitivity with ESI (Option 200) 1 pg reserpine injected on-column Signal-to-noise ratio > 10:1 Measured at m/z 609.2807 Noise = 1 × RMS

Mass resolving power Measured at m/z 1,522 after automatic tuning 22,000 FWHM procedure Mass accuracy–MS mode Measured at m/z 609.2807 using an internal reference < 1 ppm mass Spectral acquisition rate, MS mode Spectra acquisition from m/z 100 to 3,200 30 spectra/second Dynamic range In-scan dynamic range on co-eluting components Up to 5 decades Mass range m/z 25 to 20,000 Polarity switching Pos/neg switching < 1 sec Mass accuracy temperature stability–MS mode Temperature: 15 to 35 °C (59 to 95 °F) at constant Maintain 2 ppm mass accuracy within 2 °C drift temperature while setting autotune at 25 °C per hour General system specifications

Parameter Measure Single point of control Single point data system method capability with full control for HPLC system and TOF and communication between the two such that if the LC or MS experiences a not ready condition, error, or leak, the system stops injecting the sample. Wide range of ionization sources • Electrospray (ESI) • Atmospheric pressure chemical ionization (APCI) • Atmospheric pressure photoionization (APPI) • Multimode source (simultaneous ESI and APCI) • HPLC-Chip/MS interface • AP/MALDI PDF + source based upon pulsed dynamic focusing Transparent introduction of • Dual-sprayer orthogonal ESI source, with one sprayer for analytical flow and one for the reference compound reference masses • Built-in calibrant delivery system for automated introduction of reference masses • Real-time internal reference mass correction for MS operation • Internal reference mass correction for heated sources–multimode, APCI, APPI Autotune Automated optimization of ion optics and full TOF MS mass axis calibration Data mining tools One-button extraction of compound-specific spectral and chromatographic information using Find Compounds algorithms Linked software to enable quick manual Compound-based data browsing with dynamic links to spectra and chromatograms review of complex data sets Software for protein identification Full compatibility with optional Spectrum Mill for MassHunter Workstation, including optimized Data Extractor Software for verification of recombinant BioConfirm software proteins or synthetic peptides Software for walk up compound analysis Easy Access software Molecular profiling tools • Measurement of potentially relevant molecular features from one or more LC/MS analyses (MassHunter) • Differential expression analysis to reveal features that separate one sample set from another (optional MassHunter Mass Profiler) Warranty First year expanded warranty to include all labor, parts, and travel expenses. Includes preventive maintenance and certain consumables Safety • Canadian Standards Association ^CAN/CSA-C22-2 No. 61010-01-4 ^CSA/Nationally Recognized Test Laboratory (NRTL) UL 61010-1:2005 • International Electrotechnical Commission (IEC) 61010-1 • EuroNorm (EN): 61010-1 Electromagnetic compatibility CISPR11/EN55011: Group 1, Class A Sound emission EN 27779:1991 – sound pressure Lp < 70 db Power • Americas and Japan: 200 to 220 VAC; 2500 VA max, 50/60 Hz, 15 A circuit • Europe and Asia Pacific: 220 to 240 VAC; 2500 VA max, 50 Hz, 15 A circuit Operating environment Humidity: 20 to 85 % Size 83 cm long × 73 cm wide × 133 cm high Weight 260 pounds

Ordering Information

Part number Product description G6230BA 6230B Accurate Mass TOF LC/MS System*

* Includes the 6230B Accurate-Mass TOF LC/MS System with AJS (Agilent Jet Stream Technology), MassHunter Software, a PC, a monitor and printer, and service installation of the system. www.agilent.com/chem/tof The above are not standard installation specifications. Performance specifications in this document are reviewed for accuracy, but they This information is subject to change without notice. do not represent the tests and procedures performed at installation, which are described in the Agilent 6230 Time-of-Flight LC/MS © Agilent Technologies, Inc., 2012 Published in the USA, October 19, 2012 System Installation Guide, document G3335-90064 or subsequent 5991-1347EN version number. See Site Preparation Guide and Service Notes for additional product and specification information.

Agilent G6230AA/G6224AA Accurate Mass TOF LC/MS

Site Preparation Guide

Notices Warranty Safety Notices The material contained in this document is © Agilent Technologies, Inc. 2009 provided “as is,” and is subject to being CAUTION No part of this manual may be reproduced in changed, without notice, in future editions. any form or by any means (including electronic Further, to the maximum extent permitted by A CAUTION notice denotes a hazard. storage and retrieval or translation into a applicable law, Agilent disclaims all It calls attention to an operating foreign language) without prior agreement and warranties, either express or implied, with procedure, practice, or the like that, if regard to this manual and any information written consent from Agilent Technologies, not correctly performed or adhered to, Inc. as governed by United States and contained herein, including but not limited to international copyright laws. the implied warranties of merchantability and could result in damage to the product fitness for a particular purpose. Agilent shall or loss of important data. Do not Manual Part Number not be liable for errors or for incidental or proceed beyond a CAUTION notice consequential damages in connection with G2597-90034 until the indicated conditions are fully the furnishing, use, or performance of this document or of any information contained understood and met. Edition herein. Should Agilent and the user have a First edition, February 2009 separate written agreement with warranty WARNING terms covering the material in this document Printed in USA that conflict with these terms, the warranty Agilent Technologies, Inc. terms in the separate agreement shall A WARNING notice denotes a control. hazard. It calls attention to an operating procedure, practice, or the Technology Licenses like that, if not correctly performed The hardware and/or software described in or adhered to, could result in this document are furnished under a license personal injury or death. Do not and may be used or copied only in accordance with the terms of such license. proceed beyond a WARNING notice until the indicated conditions are Restricted Rights Legend fully understood and met. If software is for use in the performance of a U.S. Government prime contract or subcontract, Software is delivered and licensed as “Commercial computer software” as defined in DFAR 252.227-7014 (June 1995), or as a “commercial item” as defined in FAR 2.101(a) or as “Restricted computer software” as defined in FAR 52.227-19 (June 1987) or any equivalent agency regulation or contract clause. Use, duplication or disclosure of Software is subject to Agilent Technologies’ standard commercial license terms, and non- DOD Departments and Agencies of the U.S. Government will receive no greater than Restricted Rights as defined in FAR 52.227- 19(c)(1-2) (June 1987). U.S. Government users will receive no greater than Limited Rights as defined in FAR 52.227-14 (June 1987) or DFAR 252.227-7015 (b)(2) (November 1995), as applicable in any technical data.

2 Agilent G6230AA/G6224AA TOF Site Preparation Guide

Contents

1 General Information 6 Instrument Identification 6 Important Safety Warnings 6 Many internal parts of the 6230/6224 TOF carry dangerous voltages 6 Electrostatic discharge is a threat to TOF electronics 7 Some parts are dangerously hot 7 Chemical Safety 8 Safety and Regulatory Certifications 8 Information 8 Symbols 9 Electromagnetic compatibility 10 Sound emission declaration 10 Cleaning 10 Recycling the Product 11

2 Site Preparation 12 Overview 12 Customer Responsibility 12 Agilent responsibility 13 Activities not covered as part of installation procedure 13 Other documentation 14 Space and Weight Requirements 14 Electrical requirements 17 Voltage ranges and power requirements of major components 17 Single-phase power configurations 18 Power Configurations 19 Configuration 19 Measurement 19

Agilent G6230AA/G6224AA TOF Site Preparation Guide 3

Nominal Voltage 19 Power plugs and cords 20 Other electrical considerations 20 Communication Requirements 21 Telephone 21 Site LAN network 21 Air conditioning requirements 21 Environmental Conditions 21 Temperature and humidity 22 Airborne dust 22 Exhaust venting 22 Nitrogen Gas Requirements 24 Nitrogen purity for drying gas 24 Special installation requirement for HPLC Chip Cube configurations 25 Regulators, tubing, and fittings 26 Laboratory Supply Requirements 26 Operating supplies 26 Cleaning solvents 26 Fume hood 27 Recommended Tools 27 Data System Supplies 28 Spare parts and consumables 28 Receiving the System 30 Delivery and unloading 30 Inspecting for damage 30 Storage 31 Unpacking 31 Installation and Verification 32 Installation 32

4 Agilent G6230AA/G6224AA TOF Site Preparation Guide

Nitrogen Generator Considerations 32 System Verification 32 Sensitivity specifications 32

Appendix A: Power Cords 33

Appendix B: Nitrogen Gas Generators 35 Single-instrument gas generators 35 Nitrogen gas generator installation 35

Agilent G6230AA/G6224AA TOF Site Preparation Guide 5 General Information

1 General Information

This document is used to verify that the installation site is properly configured for the Agilent 6230/6224 TOF MS system. The site conditions must meet the minimum specifications, as outlined below, before the Agilent Customer Engineer (CE) can proceed with the TOF system installation.

Instrument Identification Each Agilent 6230/6224 Accurate Mass TOF MS is identified by a unique 10-character serial number. This serial number is located on a label on the lower left front corner of the instrument below the power switch. When corresponding with Agilent Technologies about your instrument, be sure to include the model number and the full 10- character serial number. Write the serial number of your Agilent 6230/6224 Accurate Mass TOF here for reference:

Important Safety Warnings There are several important safety notices that you should always keep in mind when using the Agilent 6230/6224 Accuate Mass TOF system.

Many internal parts of the 6230\6224 TOF carry dangerous voltages If the 6230/6224 TOF is connected to a power source, even if the power switch is off, potentially dangerous voltages exist on: • The wiring between the 6230/6224 TOF power cord and the AC power supply, the AC power supply itself, and the wiring from the AC power supply to the powerswitch. With the power switch on, potentially dangerous voltages also exist on: • All electronics boards in the instrument. • The internal wires and cables connected to these boards. • The wires for any heater.

6 Agilent G6230AA/G6224AA TOF Site Preparation Guide General Information

WARNING All these parts are shielded by covers. With the covers in place, it should be difficult to accidentally make contact with dangerous voltages. Unless specifically instructed to, never remove a cover.

WARNING If the power cord insulation is damaged, frayed, or worn, the cord must be replaced. Contact your Agilent service representative.

Interrupting the protective conductor inside or outside the 6230/6224 Accuate Mass TOF system or disconnecting the protective earth terminal can create a shock hazard for the operator and can damage the instrument.

WARNING The Agilent 6230/6224 Accurate Mass TOF LC/MS System relies on a protective earth ground for safety. The instrument must be connected to a grounded power outlet. Please refer to Appendix A for more information.

Make sure the power cords supplied with the 6230/6224 TOF are appropriate for your country and site before using them. Maintain easy access to the power cords so they can be disconnected during maintenance. The use of incorrect or makeshift fuses or the short-circuiting of fuse holders creates a shock hazard for the operator and can damage the instrument. Replace fuses only with fuses of identical current rating and type. Excessive fluctuations in the line voltage can create a shock hazard and can damage the instrument. Make sure the supply voltage does not fluctuate more than -5% or +10% from the rated voltage. This equipment must be installed in a Category II environment as defined in IEC 664.

Electrostatic discharge is a threat to TOF electronics The printed circuit (PC) boards in the 6230/6224 TOF can be damaged by electrostatic discharge. Do not touch any of the boards unless it is absolutely necessary. If you must handle them, wear a grounded wrist strap and take other antistatic precautions. Wear a grounded wrist strap any time you must remove the 6230/6224 TOF covers.

Some parts are dangerously hot Some parts of the 6230/6224 TOF operate at temperatures high enough to cause serious burns. Whenever possible, cool the part of the instrument that you will be maintaining before you begin working on it. You should always cool heated areas of the 6230/6224 TOF to room temperature before working on them. They will cool faster if you first set the temperature of the heated zone to room temperature. Turn the zone off after it has reached the setpoint. If you must perform maintenance on hot parts, use a wrench and wear gloves.

Agilent G6230AA/G6224AA TOF Site Preparation Guide 7 General Information

Chemical Safety

WARNING The foreline pump exhaust and source exhaust will contain traces of the chemicals you are analyzing. These could potentially be toxic. Vent the foreline pump exhaust and source exhaust outside your laboratory or into a fume hood. Be sure to comply with all local environmental regulations.

Safety and Regulatory Certifications The 6230/6224 Accurate Mass TOF conforms to the following safety standards: • Canadian Standards Association (CSA): CAN/CSA–C22.2 No. 61010–1–04 • CSA/Nationally Recognized Test Laboratory (NRTL): UL 61010–1:2005 • International Electrotechnical Commission (IEC): 61010–1 • EuroNorm (EN): 61010–1 The 6230/6224 Accurate Mass TOF conforms to the following regulations on Electromagnetic Compatibility (EMC) and Radio Frequency Interference (RFI): • CISPR 11/EN 55011: Group 1, Class A • IEC/EN 61326 • AUS/NZ This ISM device complies with Canadian ICES-001. Cet appareil ISM est conforme a la norme NMB—001 du Canada.

The 6230/6224 Accurate Mass TOF is designed and manufactured under a quality system registered to ISO 9001.

Information The Agilent Technologies 6230/6224 Accuate Mass TOF meets the following IEC (International Electro-technical Commission) classifications: Equipment Class I, Laboratory Equipment, Installation Category II, Pollution Degree 2. This unit has been designed and tested in accordance with recognized safety standards and is designed for use indoors. If the instrument is used in a manner not specified by the manufacturer, the protection provided by the instrument may be impaired. Whenever the safety protection of the 6230/6224 TOF has been compromised, disconnect the unit from all power sources and secure the unit against unintended operation.

8 Agilent G6230AA/G6224AA TOF Site Preparation Guide General Information

Refer servicing to qualified service personnel. Substituting parts or performing any unauthorized modification to the instrument may result in a safety hazard.

Symbols Warnings in the manual or on the instrument must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions violates safety standards of design and the intended use of the instrument. Agilent Technologies assumes no liability for the customer’s failure to comply with these requirements. Symbols used in this manual:

See accompanying instructions for more information

Indicates a hot surface.

Indicates hazardous voltages.

Indicates earth (ground) terminal.

Indicates electrostatic discharge hazard.

Indicates that you must not discard this electrical/electronic product in domestic household waste.

Agilent G6230AA/G6224AA TOF Site Preparation Guide 9 General Information

Electromagnetic compatibility This device complies with the requirements of CISPR 11. Operation is subject to the following two conditions: • This device may not cause harmful interference. • This device must accept any interference received, including interference that may cause undesired operation. If this equipment does cause harmful interference to radio or television reception, which can be determined by turning the equipment off and on, the user is encouraged to try one or more of the following measures: 1 Relocate the radio or antenna. 2 Move the device away from the radio or television. 3 Plug the device into a different electrical outlet, so that the device and the radio or television are on separate electrical circuits. 4 Make sure that all peripheral devices are also certified. 5 Make sure that appropriate cables are used to connect the device to peripheral equipment. 6 Consult your equipment dealer, Agilent Technologies, or an experienced technician for assistance. 7 Changes or modifications not expressly approved by Agilent Technologies could void the user’s authority to operate the equipment.

Sound emission declaration Sound pressure Sound pressure Lp < 70 dB according to EN 27779:1991. Schalldruckpegel Schalldruckpegel LP < 70 dB am nach EN 27779:1991.

Cleaning To clean the unit, disconnect the power and wipe down with a damp, lint-free cloth.

10 Agilent G6230AA/G6224AA TOF Site Preparation Guide General Information

Recycling the Product For recycling, contact your local Agilent sales office.

Agilent G6230AA/G6224AA TOF Site Preparation Guide 11 Site Preparation

2 Site Preparation

Overview This section describes how to properly prepare your site for a new 6230/6224 Accuate Mass TOF . Follow these instructions carefully as delays due to improper site preparation may result in loss of instrument use during the warranty period. Before the 6230/6224 Accuate Mass TOF system can be installed, the site must be properly prepared. Site preparation includes, but is not limited to, ensuring that adequate facilities are available. Among the site requirements are: • Adequate space is available for the 6230/6224 Accuate Mass TOF system. • A suitable supporting bench is available. • Adequate electrical power is available at the correct voltages and frequencies. • Environmental control systems are adequate to maintain a correct, stable operating environment. • An adequate source of clean, dry nitrogen gas for up to 18 L/min of continuous flow. • Adequate exhaust venting for up to 20 L/min of nitrogen gas and vapor from the 6230/6224 Accuate Mass TOF system. • Preparations for safe exhaust venting are adequate. • Supplies necessary for instrument operation are available, including solvents, carrier and reagent gasses, and printer paper.

WARNING Do not stack the LC modules on top of the TOF system. This arrangement is potentially unstable and dangerous.

Delays due to inadequate site preparation could cause loss of instrument use during the warranty period. In extreme cases, Agilent Technologies may ask to be reimbursed for the additional time required to complete the installation. Agilent Technologies provides service during the warranty period and under maintenance agreements only if the specified site requirements are met.

Customer Responsibility Unless previous arrangements have been made with Agilent Technologies, site preparation is the customer’s responsibility. Customer responsibilities include, but are not limited to: 1 Planning, scheduling, and preparing the site according to the specifications in this manual

12 Agilent G6230AA/G6224AA TOF Site Preparation Guide Site Preparation

2 Verifying that the electrical environment is safe and adequate for the 6230/6224 TOF system installation and operation 3 Complying with all local laws (codes, ordinances, and regulations) for mechanical, building, and electrical distribution systems, hazardous waste disposal, and chemical storage Compliance must exist prior to installation 4 Providing lifting equipment adequate to unload the system from the delivery vehicle and transport it to the site where it will be installed 5 Providing at least four people to help lift the 6230/6224 TOF onto the laboratory bench 6 Providing adequate secure storage space for the system until it can be installed by an Agilent Technologies representative

Agilent responsibility An Agilent Technologies service representative will install the 6230/6224 TOF and verify its performance. The service representative’s responsibilities are limited to: 1 Unpacking the 6230/6224 Accuate Mass TOF system and verifying that all components are present and undamaged. 2 Connecting the carrier gas line to the instrument from the tank, regulators, and lines previously installed by the customer. 3 Installing, connecting, and turning on 6230/6224 Accuate Mass TOF system components. 4 Verifying that the system meets Agilent Technologies performance standards. 5 Providing basic user familiarization for system hardware and software. 6 Signing the customer up through the Response Centers for instrument and software support.

Activities not covered as part of installation procedure Agilent Technologies is not responsible for: • Any task not listed in the Agilent 6230/6224 Accuate Mass TOF system Installation Manual or in the installation guides for the LC, data system, and other accessories • Installing a nitrogen gas generator and/or air compressor unless additional installation time is purchased • Connecting, or verifying the performance of hardware and software not provided by Agilent Technologies

Agilent G6230AA/G6224AA TOF Site Preparation Guide 13 Site Preparation

NOTE Items that do not include installation by Agilent Technologies must be installed by the customer.

• Testing the 6230/6224 Accuate Mass TOF system using customer standards or samples • Detailed instruction in the operation of the computer operating system and MassHunter Workstation software.

NOTE Contact Agilent Technologies for information concerning training classes

• Setting up laboratory procedures. Assistance with laboratory procedures can be obtained from an Agilent Applications Engineer (AE) on a consulting basis at additional cost.

Contact Agilent Technologies for information concerning assistance with NOTE laboratory procedures, application development, or chemical analysis consulting.

• Operation of the 6230/6224 Accuate Mass TOF system following installation

Other documentation Additional information is contained in the following documentation: • Agilent 6230/6224 Accuate Mass TOF system Users Manual • Agilent 6230/6224 Accuate Mass TOF system Hardware Installation manual • 1200 Series User manuals • G3335AA MassHunter Workstation software manuals and online help • The appropriate sensitivity specifications for your instrument. See the Agilent Technologies Web site at: http://www.agilent.com/chem

Space and Weight Requirements The Agilent 6230/6224 Accuate Mass TOF system is designed for indoor use at altitudes of up to 2,000 meters. Table 1 lists dimension and weight information for the 6230/6224 TOF ystem and related components. The installation site must have enough bench space for the 6230/6224 Accuate Mass TOF system, LC system, data system and accessories. In addition, there must be sufficient space around the instrumentation for air flow and maintenance access. At least 30 cm (12 in) to the left of the 6230/6224 TOF and at least 15 cm (approximately 6 inches) behind the 6230/6224 TOF must kept clear.

14 Agilent G6230AA/G6224AA TOF Site Preparation Guide Site Preparation

The supporting surface must be sturdy enough to support the weight of the entire 6230/6224 Accuate Mass TOF system and any accessories.

WARNING Do not stack the LC modules on top of the 6230/6224 Accurate Mass TOF system. This arrangement is potentially unstable and dangerous.

The rough pump must be located under the laboratory bench or on the floor. It must be within the 200 cm (79 in) length of the vacuum hose. The hose exits only the back of the 6230/6224 TOF. The hose is metal and cannot be bent sharply.

CAUTION Do not put the rough pump on your laboratory bench with the 6230/6224 TOF due to the vibration that the pump creates. This vibration can lead to a loss of mass accuracy and resolution.

CAUTION The supporting surface for the 6230/6224 Accurate Mass TOF system should be kept relatively vibration free.

The 6230/6224 TOF spray chamber must be connected to the spray chamber waste bottle which must be positioned below the 6230/6224 TOF instrument. The drain bottle is supplied with a 180 cm (72 in) teflon hose. A hose extension kit (G1946-67002) is available to add 120 cm (48 in) to the drain hose length. Please refer to Table 1 for further information regarding totals weights for the 6230/6224 TOF, rough pump, and various accessories.

Table 1 Product dimensions 1

Product Dimensions, cm (in) Weight kg (lbs) Height Width Depth 6230/6224 TOF dimensions2 133 73 (28.74) 73 (28.74) 114 (252) E2M28 Rough pump 25.6 (10.1) 17 (6.7) 58.3 (23.0) 44.5 (98) Agilent Jet Steam G3251B Dual-spray ESI source3 17 (6.8) 18 (7.1) 9.5 (3.7) 1.7 (3.7) G3252B APCI source3 23 (9.2) 18 (7.1) 13.0 (5.1) 1.7 (3.7) G1978B Multimode Source3 23 (9.2) 18 (7.1) 13.0 (5.1) 5.05 (2.29) G4240B HPLC Chip Cube 35.9 (14.1) 34.9 (13.7) 29.8 (11.7) 14 (31)

Agilent 1100/1200 Series LC Size and weight of the LC system depends on the number and System type of modules included. Most Agilent 1100/1200 Series LC modules are approximately 35 cm (14 in) wide and 45 cm (18 in) deep.

Data System Size and weight of the Data system depends on the components included. Reserve at least 100 cm (39 in) of bench space for the data system. A typical data system weight is 23 kg (50 lb).

Agilent G6230AA/G6224AA TOF Site Preparation Guide 15 Site Preparation

1 The 6230/6224 TOF requires a source of nitrogen gas. Typically, this is either a 160-liter Dewar of liquid nitrogen or a nitrogen generator. Be sure to plan for the space and utilities your nitrogen source may require.

2 The 6230/6224 TOF dimensions represent the maximum cabinet dimensions with a G3251B Dual- Spray ESI source installed. At least 30 cm (1 ft) to the left (source end) of the instrument must be added to these dimensions to provide adequate instrument access. The supporting surface must be relatively vibration free and capable of supporting the combined weight of the TOF system.

3 The various API (atmospheric pressure ionization) sources attach to the left side of the 6230/6224 TOF. Their depths must be added to the width of the 6230/6224 TOF. Heights for the interfaces include the height of the nebulizer.

Please refer to Figures 1 and 2 for suggested system configurations.

Figure 1 Suggested configuration 1

Figure 2 Suggested configuration 2

16 Agilent G6230AA/G6224AA TOF Site Preparation Guide Site Preparation

Electrical requirements

The customer is responsible for providing appropriate electrical power and power outlets for all of the components and accessories used with the 6230/6224 Accuate Mass TOF system. Power considerations include: • Voltage ranges of major components • Power requirements of major components • Single-phase power configurations • Power plugs, receptacles and cords

Voltage ranges and power requirements of major components Table 2 lists the voltage ranges and power requirements for the 6230/6224 TOF and related equipment. Extra power capacity for future additions is strongly recommended.

NOTE: Each product listed requires a dedicated circuit. The TOF, LC, and data system should each have a separate circuit breaker. The TOF system includes a wide-range power supply that can operate without reconfiguration, however the rough pump model must be configured for either of two voltage ranges of single-phase alternating current (ac): • 200 - 210 Vac +10%, -5%, 50/60 Hz (typical for the U.S. and Japan) • 220 - 240 Vac Vac +10%, -5%, 50 Hz (typical for Europe and Australia)

The rough pump will be configured according to the standard voltage and frequency in the country from which the order originates. For example, if an order originates from an Agilent sales office in Germany, the rough pump will be configured for the standard electrical power in Germany.

CAUTION If an instrument is being ordered from one location, but is to be installed in another location with different electrical power characteristics, a special note must be made on the order that the electrical power at the site is different from the standard electrical power in that country.

Power must meet the stated stability specifications. Use a line monitor to check the power stability. If the line power is unstable, it may be necessary to install a line conditioner.

Agilent G6230AA/G6224AA TOF Site Preparation Guide 17 Site Preparation

Table 2 Voltage and Power requirements 1 Line voltage Maximum Supply Product No. of continuous AC circuit outlets power rating 6230/6224 Accuate 200 – 210 Vac2 50/60 Hz 2500 VA 15 A 1 Mass TOF system2 (U.S. & Japan) 6230/6224 Accuate 220 - 240 Vac2 50 Hz 2500 VA 15 A 1 Mass TOF system2 (Europe) 1200 Series LC 100-120 or 220-240 Vac2 800-1200 VA3 15 A 4 to 63 50/60 Hz 6230/6224 TOF Data 100-120 or 220-240 Vac2 1000 V3 15 A 4 to 6 System 50/60 Hz 1 Excessive fluctuations in the voltage of the power supply can create a shock hazard and can damage the instrument. Make sure the supply voltage does not fluctuate more than -5% or +10% from the rated voltage. This equipment must be installed in a Category II environment as defined in IEC 60664. 2 The rough pump is supplied for the standard voltage in the country where the order originates. The Agilent G3251B Dual-spray ESI source and the Agilent G3252B APCI source draw their power from the TOF instrument and do not require separate line voltage. 3 Depends on product configuration.

Single-phase power configurations

Electrical power for the 6230/6224 TOF may be delivered in either single- phase or 208-Wye configuration. Table 3 lists the primary voltage characteristics of each configuration. Correct grounding for the 208-Wye configuration must be verified by an electrician. The neutral wire cannot be used for safety grounding. The ground wire should carry zero current except for ground-fault current or static electric discharge. The entire system should share an isolated, noise-free electrical ground that is connected to the main earth ground for the facility.

WARNING The power source to which you connect a 6230/6224 TOF must be equipped with a protective earth contact (ground).

WARNING Do not interrupt the protective earth contact inside or outside the 6230/6224 TOF or disconnect the protective earth terminal (ground).

18 Agilent G6230AA/G6224AA TOF Site Preparation Guide Site Preparation

Table 3 Power Configurations Configuration Measurement Nominal Voltage

Single phase Line to neutral 200, 220, 230, or 240 Vac1

Line to ground 200, 220, 230, or 240 Vac1

Ground to neutral < 0.5 V rms

208-Wye Line to line (phase A to 208, 220 Vac phase B) Line to ground (phase A 120, 127 Vac to ground) Line to ground (phase B 120, 127 Vac to ground) 1 Varies with country. Single phase, 60 Hz operation is not supported for nominal voltage above 220 Vac.

Agilent G6230AA/G6224AA TOF Site Preparation Guide 19 Site Preparation

Power plugs and cords

The 6230/6224 TOF is supplied with an appropriate power cord and plug for the country in which the order originates. See Appendix A on page 33 for illustrations of the available power cords. The length of all 6230/6224 TOF power cords is approximately 2.4 m (8 ft).

The 6230/6224 TOF data system components also include power cords with plugs appropriate for the country in which the order was placed. Power cord lengths for the data system components and accessories are approximately 2.3 m (7.5 ft).

WARNING Make sure the power cords supplied with the 6230/6224 TOF are appropriate for your country and site before using them. See Appendix A for a description of power cords used by country.

WARNING Do not use extension cords with the 6230/6224 TOF. Extension cords cannot provide enough power and can be a safety hazard.

Other electrical considerations Additional electrical considerations include: • Electromagnetic interference (EMI), such as is generated by NMRs, radio transmitters, and microwave links, may interfere with system performance. • Protect the system from static electricity by observing humidity and temperature requirements. Minimize the presence of non- conductive products such as carpets and vinyl floor tiles. • Install emergency-off pushbuttons that can disconnect power to the ventilation system and all electric equipment in the room except overhead lighting. • Separate convenience outlets should be provided for building maintenance and other appliances. Convenience outlets must be on circuits separate from the 6230/6224 Accuate Mass TOF system. Convenience outlets must share the normal building distribution ground, not the 6230/6224 Accuate Mass TOF system ground. • In some geographical areas it may be advisable to install lightning protection for personnel and equipment.

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Communication Requirements Telephone A telephone with a long cord or a cordless phone to be used at the computer will allow the operator to communicate with Agilent Technologies support personnel.

Site LAN network If you intend to connect your 6230/6224 Accuate Mass TOF system to your site’s LAN network, you must have an additional shielded twisted pair network cable. In addition, internet access for the control PC is strongly recommended to allow remote control and diagnosis of the 6230/6224 TOF through WebEx or Carbon Copy software. This is required for some service contracts.

Agilent Technologies is not responsible for connecting to or establishing NOTE communication with your site LAN network. The service representative will test the 6230/6224 TOF’s ability to communicate with the bundled PC ONLY.

The IP address for the 6230/6224 TOF is fixed and cannot be changed. Placing NOTE the 1200 LC or the 6230/6224 TOF on a corporate LAN environment is not supported.

Air conditioning requirements Air conditioning considerations include temperature, humidity, airborne dust, and exhaust venting. Each of these is considered in more detail in the following material.

Environmental Conditions The 6230/6224 TOF is designed for use in the following environmental conditions for Class 1 Laboratory Equipment. Please see Table 4 for a summary of environmental conditions.

Table 4 Environmental Conditions Equipment class: Class 1 Laboratory Equipment Pollution: Degree 2 Installation: Category II Environment: Indoor Use Altitude: Not to exceed 2000 m Electrical Supply: 200 - 210 Vac, 50/60 Hz (configuration for U.S. & Japan) 220 - 240 Vac, 50 Hz (configuration for Europe)

Agilent G6230AA/G6224AA TOF Site Preparation Guide 21 Site Preparation

Mains supply voltage: Fluctuations not to exceed 10% of nominal supply voltage Operating Temperature: 15 to 35°C (59 to 95°F) at constant temperature +/- 3°C from calibration temperature Humidity: 20-85% RH at 35 °C

Temperature and humidity The Agilent 6230/6224 TOF TOF is specified for operation under the following conditions: • 15 to 35°C (59 to 95°F) • Constant temperature (variations < 3°C/hr) • 20-85% relative humidity at 35°C • Non-condensing, non-corrosive atmosphere Environmental control systems must maintain these temperatures and humidity ranges. The 6224 TOF dissipates up to 910 Watts (3,107 BTU/hr). The 6230 TOF dissipates up to 1120 Watts (3,824 BTU/hr). Approximately 600 Watts (2,047 BTU/hr) are removed with the source exhaust. The LC and data system also contribute significantly to the cooling load. The exact amounts will depend on their configurations. Additional allowances should be made for other heat sources such as heat from other equipment, heat from adjacent rooms, and heat from laboratory personnel.

Airborne dust Agilent recommends a maximum airborne particle density of 55 mg/m3. If you suspect your site exceeds this limit, contact your local Agilent Customer Service Organization. Agilent Customer Engineers with special training and equipment can test for airborne particle density. They can offer suggestions for reducing airborne dust.

Exhaust venting There are two sources of exhaust on the 6230/6224 TOF: the spray chamber and the rough pump. The drain hose routes both nitrogen gas and vapor from the spray chamber to the drain bottle. The vapor is made up of mobile phase and sample. The spray chamber and rough pump vent up to 20 liters/minute of nitrogen gas and vapor. The rough pump exhaust contains traces of solvent, sample, and hydrocarbon pump fluid. The mist filter on the rough pump traps the majority of the pump fluid vapor. It does not trap the traces of solvent and sample that may be present in the exhaust gases.

22 Agilent G6230AA/G6224AA TOF Site Preparation Guide Site Preparation

WARNING User safety requires that the spray chamber exhaust and the rough pump exhaust are vented externally to the building and not recirculated by the environmental control system. Exhaust gas venting must comply with all local environmental codes. Health hazards include chemical toxicity of solvents, samples, buffers, pump fluid vapor, and aerosolized biological samples.

WARNING Failure to vent the rough pump and spray chamber separately will void the warranty for the 6230/6224 TOF. Agilent service representatives will not install an Agilent 6230/6224 TOF until an adequate exhaust system is present and functioning. See the caution statement below for further details.

CAUTION The spray chamber exhaust and rough pump exhaust must be vented using separate lengths of exhaust tubing. These may be connected into a common exhaust manifold. This is to minimize the chances of rough pump fluid vapor entering the spray chamber when drying gas is not flowing.

The combined exhaust flow from the spray chamber and rough pump is up to 20 l/min of gas and vapor. Flow is continuous as long as the instrument is on. Both exhaust vents must be at or slightly below atmospheric pressure (negative pressure) with an exhaust flow of 20 l/min. If a negative pressure vent is not available, the length of the tubing from the rough pump and the drain bottle to the vent should each not exceed 460 cm (15 ft).

CAUTION Positive pressure in the spray chamber exhaust tubing and drain bottle can affect instrument performance and may contribute to excessive background contaminant levels.

Agilent G6230AA/G6224AA TOF Site Preparation Guide 23 Site Preparation

Nitrogen Gas Requirements The Agilent 6230/6224 TOF requires a very large quantity of high- purity nitrogen for drying gas, nebulizing gas, and to pressurize the calibrant delivery system.

WARNING Use only nitrogen for the drying and nebulizing gas. Use of air, oxygen, or other gases when combined with volatile solvents and high voltages in the spray chamber, could result in an explosion. Use of air, oxygen, or other gases may also cause deterioration of parts in the 6230/6224 TOF and have a negative effect on instrument operation and sensitivity.

Systems with G4240A HPLC Chip Cube source will need air to help lower NOTE background counts. See the section Special installation requirement for HPLC Chip Cube configurations.

Due to the large volumes of nitrogen required for instrument operation, the use of a nitrogen generator (gas separator) or a large (160 liters or greater) dewar of liquid nitrogen is recommended. Agilent Technologies offers several nitrogen gas generators well suited for use with the 6230/6224 TOF. See Appendix B on page 35 for more detailed information about these generators. Liquid chromatographs other than the Agilent 1100 or 1200 Series LC may require compressed gases for the LC autosampler and for sparging the LC solvents. If you are installing a liquid chromatograph other than the Agilent 1100 or 1200 Series LC, see the site preparation and installation material supplied with that LC.

WARNING Contact your gas supplier for handling and safety information for the gases you use. Compressed or liquefied gases can be dangerous.

Nitrogen purity for drying gas The nitrogen gas used for drying gas must be free of contaminants. Table 5 lists specifications for the nitrogen. Purity specifications vary according to the source of the nitrogen. This is due to the different types of contaminants common to different nitrogen sources. Bottled nitrogen tends to be contaminated with hydrocarbons which can severely degrade 6230/6224 TOF sensitivity. Even with the gas conditioner (purifier) supplied with the TOF, it is virtually impossible to remove all of the hydrocarbon contaminants if the bottled nitrogen is not at least 99.5% pure to start with. Oxygen is the primary contaminant of gas supplied by a nitrogen generator. Oxygen has less impact on 6230/6224 TOF sensitivity, and in some operating modes and certain applications may enhance sensitivity in small concentrations. Therefore, the purity specification for gas from a nitrogen generator can be less stringent.

24 Agilent G6230AA/G6224AA TOF Site Preparation Guide Site Preparation

A high-capacity gas conditioner is supplied with the 6230/6224 TOF. Its primary function is to remove hydrocarbon contamination from the nitrogen before it reaches the TOF system. This conditioner has 1/4- inch Swagelok fittings.

Table 5 Nitrogen gas requirements

Source Purity Gas Pressure Flow Nitrogen generator or 95% pure3 or better 5.5 to 6.8 bar Up to 18 liters/minute4 liquid nitrogen and hydrocarbon free2 (80 to 100 psi) (1080 liters/hour) High pressure bottled 99.5% pure1 or better 5.5 to 6.8 bar Up to 18 liters/minute4 Nitrogen and hydrocarbon free2 (80 to 100 psi) (1080 liters/hour) (Not supported due to high usage of nitrogen) 1 With the remaining gas being oxygen. 2 Less than 0.1 parts per million of hydrocarbons. 3 With the remaining gas being oxygen and trace argon. 4 At least 3 liters/minute is required at all times to prevent air from entering the instrument.

Special installation requirement for HPLC Chip Cube configurations For HPLC Chip Cube installation, high purity air is required. The high purity air is used to reduce background ions in the spray chamber. Please see Table 7 for a summary of the air for Chip Cube requirements.

Table 7 Air for Chip Cube requirements

Source Purity Gas Pressure Flow High pressure 99.99% pure or better and 5.5 to 6.8 bar 1 4 liters/minute bottled Air hydrocarbon free1 (80 to 100 psi) (0.06 liter/hour) Air Compressor 99.99% pure or better and 5.5 to 6.8 bar 1 4 liters/minute hydrocarbon free1 (80 to 100 psi) (0.06 liter/hour) 1 Inlet gas pressure for air must equal the pressure used for the nitrogen gas supply pressure.

Agilent G6230AA/G6224AA TOF Site Preparation Guide 25 Site Preparation

Regulators, tubing, and fittings You must supply an appropriate regulator for your source of nitrogen gas. The regulator must be able to supply gas in the specified pressure range. It must have one outlet with 1/4-inch Swagelok fittings. Gas generators have built-in regulators so they do not require an external regulator. A dewar of liquid nitrogen typically requires a single-stage regulator (see the dewar manufacturer’s literature for specifics). Nitrogen from a house supply requires a single-stage regulator if the supply is at a pressure higher than the specified range. Bottled, compressed nitrogen typically requires a dual-stage regulator. See the Agilent Chemical Analysis Columns and Supplies Catalog (5988- 4970EN) for dual-stage regulators available from Agilent Technologies. The 6230/6224 TOF is supplied with 300 centimeters of heavy-wall 1/4-inch Teflon tubing to connect the nitrogen supply to the 6230/6224 TOF . You may need to supply additional heavy-wall 1/4-inch Teflon tubing if the nitrogen supply is located farther from the TOF system. You can substitute 1/4-inch medical-grade polypropylene tubing for the Teflon tubing. You must supply fittings, ferrules, and connectors of a Swagelok design for the 1/4-inch tubing.

Laboratory Supply Requirements Operating supplies Routine operation of the 6230/6224 TOF requires the following supplies: • Acetonitrile, pesticide residue analysis (PRA) grade or better • Methanol, PRA grade or better • Water, Nanopure/HPLC grade or better • Ammonium formate, 97% purity or better • Acetic acid, 99.7% minimum purity • Formic acid, 95% minimum purity

It is recommended that the highest grade solvents and chemicals available be NOTE used with the 6230/6224 TOF. Lower purity grades will result in higher background counts seen in the 6230/6224 TOF.

Cleaning solvents Cleaning tasks for the 6230/6224 TOF require the following HPLC- grade or better solvents: • Isopropyl alcohol • Methanol

26 Agilent G6230AA/G6224AA TOF Site Preparation Guide Site Preparation

• Water Proper storage, handling, and disposal of these chemicals is required for personal and environmental safety.

WARNING Contact your chemical supplier for solvent handling and safety information. Chemical solvents should be considered hazardous and must be handled with care.

Fume hood An auxiliary work space and fume hood are needed for some maintenance procedures.

Recommended Tools Table 8 lists the basic hand tools required for maintenance of the 6230/6224 Accuate Mass TOF system.

Table 8 Commonly used tools

Description Part Number Adjustment fixture for nebulizer G1946-20215 1 Nebulizer magnifier G1946-80049 1 Plastic tubing cutter 8710-1930 1 Pliers, needle nose 8710-0004 Safety glasses 9300-1159 1

Screwdrivers flat blade, large 8730-0002 Flat blade, pocket 8730-0008 1 6mm Allen T Driver, 200mm 8710-2549 1 Torx T-6 8710-2548 1 Torx T-10 8710-1623 1 Torx T-20 8710-1615 1

Wrenches 3-mm, open end 8710-2699 1 1/4-inch x 5/16-inch, open end 8710-0510 1 1/2-inch x 7/16-inch, open end 8710-0806 1 1/2-inch x 9/16-inch, open end 8710-0877 1 Hex key, 6 mm 8710-1839 1 Torx T-25 “L” wrench 8710-1817 1 1 Included in the shipping kit supplied with the 6230/6224 TOF.

Agilent G6230AA/G6224AA TOF Site Preparation Guide 27 Site Preparation

Data System Supplies You will need paper for printing the results of the testing done during installation and later for printing reports of your analyses. You may also need appropriate media such as floppy disks, tape cartridges, or writable DVDs for making backup copies of your data files.

Spare parts and consumables Table 9 lists recommended spare parts and consumables used in the operation and maintenance of an Agilent 6230/6224 Accuate Mass TOF system. Keeping these parts on hand can reduce system downtime related to instrument maintenance and repair. Table 9 Recommended spare parts and consumables

Chemical consumables Part Number Calibrant solutions ES TOF calibrant solution G1969-85000 ESI TOF reference solution G1969-85001 APCI TOF calibrant solution G1969-85010 Multimode for TOF calibrant solution G1969-85020

Performance standards ES/APCI positive ion performance standard (reserpine) G2423A

Gas filters Nitrogen drying gas conditioner (purifier) BHT-4 Maintenance kit for pre-filter cartridges for 5183-2003 or 5183-2004 5183-2014 nitrogen gas generators

General supplies Abrasive mesh (micro-grit paper) 8660-0827 Cloths, clean, lint-free 05980-60051 Cotton swabs 5080-5400

Agilent 6230/6224 TOF spare parts Glass Capillary 59987-20040 Spring for capillary cap 1460-2571 H.T. capillary cap G1946-20301 Capillary seal, front, 1/4-inch id 0905-1475 Corona needle G1947-20029 Corona needle holder G1947-60103 TOF MCP Detector G1969-80224 Rough pump oil, Inland 45, 1 liter 6040-0834 Rough pump oil, Inland 45, 1 gallon 6040-0798 Inlet filter assembly G1946-60180 Replacement 5 micron filter element for inlet filter assembly (5 ea) 0100-2051 Fuse, 8A, Time Delay 2110-0969 Fuse, 12.5A, Time Delay 2110-1398

28 Agilent G6230AA/G6224AA TOF Site Preparation Guide Site Preparation

Nebulizer needles & ferrules Electrospary Nebulizer Assembly G1946-60098 Electrospray & reference nebulizer needle kit G2427A APCI nebulizer needle kit G2428A Needle holder (included with ES & APCI nebulizer needle kit) G1946-20147 Ferrule for nebulizer needle (included with ES & APCI nebulizer G1946-20213 needle kit)

Agilent G6230AA/G6224AA TOF Site Preparation Guide 29 Site Preparation

Receiving the System When your 6230/6224 Accuate Mass TOF system is delivered, it is your responsibility to provide for removal of the shipping containers from the truck and their storage until installation. Contact your Agilent service representative as soon as your shipment arrives to arrange an installation date.

Delivery and unloading The TOF shipping containers are large and very heavy. Table 10 lists the dimensions and weight of the TOF base unit and TOF flight assembly shipping containers. Additional shipping containers contain the TOF shipping kit and PC data system.

Table 10 TOF Shipping Containers

Description Width Depth Height Weight TOF base unit 73 cm (28.74 in) 73 cm (28.74 in) 63 cm (24.8 in) 114.5 kg (252 lbs) TOF flight assembly 84 cm (33 in) 53 cm (21 in) 122 cm (48 in) 39 kg (85 lbs)

The shipping containers require a loading dock and a fork lift or similar lifting device. If no loading dock and/or suitable lifting device is available, the containers cannot be removed from the delivery truck. If you make prior arrangements with your Agilent service representative, the system can be delivered in a lift-gate truck. This removes the need for a loading dock, but a lifting device is still required to move the containers.

CAUTION The shipping containers must be kept upright at all times to prevent damage to the instrument. All doorways, hallways, floors, and elevators must be able to accommodate the largest, heaviest container.

Inspecting for damage Once the shipping containers are unloaded, examine them for any obvious external damage. If any of the containers appear damaged, note on the carrier's bill of lading that there is Apparent damage - subject to inspection and test. Arrange for both the carrier's claims representative and your Agilent Technologies service representative to be present when the containers are unpacked. Do not open any of the shipping containers unless a representative of Agilent Technologies is present. Opening any of the containers without an Agilent Technologies representative present will void the receiving warranty on the instrument.

30 Agilent G6230AA/G6224AA TOF Site Preparation Guide Site Preparation

Storage It is your responsibility to store the containers until installation. If your site does not have adequate storage space, the containers may be stored at your expense in a bonded warehouse. Allow space for data system and accessory containers too. The environment in the storage area should be between 5 °C and 50 °C (41 °F and 122 °F), 20% to 80% relative humidity, non-condensing and non-corrosive. There are chemicals that are shipped with the system that may also require refrigeration between 4 and 8°C. Additionally, some of these chemicals should not be exposed to tempeartures below 5°C (41 °F)

Unpacking Do not open any shipping containers until an Agilent representative is present. Warranty claims for missing items will not be honored unless an Agilent representative is present to verify the contents of each container as it is unpacked. With the exception of the flight tube shipping insert (part number G1969-60055), the actual shipping containers become your property and should not be returned to Agilent Technologies. The flight tube shipping insert should be returned to Agilent Technologies using the prepaid shipping paperwork included in the flight tube shipping box.

Agilent G6230AA/G6224AA TOF Site Preparation Guide 31 Site Preparation

Installation and Verification Installation Once the installation has begun, it should progress in a timely manner to completion. Delays due to inadequate site preparation could cause loss of instrument use during the warranty period. In extreme cases, Agilent Technologies may ask to be reimbursed for the additional time required to complete the installation. The 6230/6224 TOF base unit is too heavy (approximately 150 kg / 350 lb) to be lifted by one person. At least four people must be available to help lift the 6230/6224 TOF onto the laboratory bench. The primary user of the 6230/6224 TOF should be present during installation to receive familiarization instruction from the Agilent service representative.

Nitrogen Generator Considerations Installation is not included with the purchase of a nitrogen generator. If you do not want to install the generator yourself, you must purchase additional Agilent Customer Engineer time. An additional travel charge is required only if the nitrogen generator is ordered after the 6230/6224 TOF is installed. In addition, you are also responsible for the availability of compressed air to supply the nitrogen generator; including, if necessary, the installation of an air compressor. The air that is supplied to the nitrogen generator must be free of hydrocarbons. Please see Appendix B of this manual for additional information.

System Verification The final step in the installation process is system verification. Your Agilent service representative will test the system against Agilent standards as documented in the Agilent 6230/6224 TOF LC/MS System Installation Manual or in the installation guides for the for the 6230/6224 TOF accessories you have purchased. The Agilent service representative will not test your system against your standards or samples. Furthermore, the Agilent Customer Engineer will not set up your laboratory procedures. Assistance with laboratory procedures can be obtained from your local Agilent Applications Engineer (AE) on a consulting basis at an additional cost.

Sensitivity specifications See the appropriate sensitivity specifications for your instrument type using the Agilent Technologies Web site at: http://www.chem.agilent.com/

32 Agilent G6230AA/G6224AA TOF Site Preparation Guide

Appendix A: Power Cords

This appendix shows the power cords available for the Agilent 6230/6224 TOF. See page 10 for information about ensuring that the correct power cord is supplied with your system.

US and Canada, NEMA 6-15P (Agilent Part Number 8120-8623)

An alternative power cord (G1946-60066) with a NEMA L6-30P connector is available at extra cost. It is useful if a twist-lock plug is desired.

European Power, CEE 7/7 (Agilent Part Number 8120-8621)

UK / Hong Kong / Singapore, BS 1363 (Agilent Part Number 8120-8620)

Switzerland / Denmark, IEC 309 (Agilent Part Number 8120-8622)

Agilent G6230AA/G6224AA TOF Site Preparation Guide 33

Australia, (Agilent Part Number 8120-8619) China, (Agilent Part Number 8121-0070)

India / South Africa, BS 546 (Agilent Part Number 8121-0710)

Israel, SI 32 (Agilent Part Number 8121-0161)

Japan, NEMA L6-20P (Agilent Part Number 8120-6903)

Taiwan, NEMA 6-20P (Agilent Part Number 8120-6360)

Korea, 15A, 2.5 M (Agilent Part Number 8121-1222)

(Not Shown)

34 Agilent G6230AA/G6224AA TOF Site Preparation Guide

Appendix B: Nitrogen Gas Generators

Agilent Technologies offers several nitrogen gas generators that are well suited to supplying a 6230/6224 TOF with nitrogen. In these generators, clean, compressed air is passed through hollow-fiber membranes that separates it into a concentrated nitrogen output stream and an oxygen-enriched permeate stream. The nitrogen is supplied to the 6230/6224 TOF while the oxygen is vented from the generator.

Single-instrument gas generators Table 11 lists the physical and performance specifications for the Agilent nitrogen gas generator (5183-2003) and the Agilent nitrogen gas generator with oxygen analyzer (5183-2004). These gas generators can supply sufficient nitrogen for a single 6230/6224 TOF. The oxygen analyzer is especially helpful in negative ion APCI operation where small concentrations of oxygen can improve performance for some analyses. These two generators require up to 60 L/min of clean, oil-free air at a pressure of at least 760 kPa (110 psi). You must have either house compressed air or an oil-free air compressor that can meet these requirements. Each generator includes two pre-filters for removing particulates and excess water vapor from the air.

Table 11 Specifications for single-instrument nitrogen gas generators Nitrogen purity 95.0% - 99.5% 1 Minimum/maximum air input pressure 415/1000 kPa (60/145 psi) Max air consumption 60 l/min at 760 kPa (110 psi) 2 Dimensions (height x width x depth) 127 cm x 40 cm x 40 cm (50 in x 16 in x 16 in) Shipping weight 34 kg (75 lbs) Electrical power (5183-2003) none Electrical power (5183-2004) 100-120 or 220-240 Vac ± 10%, 50/60 Hz 1 Varies with the input pressure and output flow required. Purity of 97% can be achieved at the maximum nitrogen output required for 6230/6224 TOF operation.

Nitrogen gas generator installation Installation is not included in the price of a nitrogen gas generator. Detailed installation, operation, and maintenance instructions are included with each generator. If you do not want to install the generator yourself, you must purchase additional installation time. See page 32 for more information. The customer is responsible for the installation of any air compressor that is needed.

Agilent G6230AA/G6224AA TOF Site Preparation Guide 35

Table 12 Nitrogen gas generator ordering information

Single-instrument generators Part Number Nitrogen gas generator 5183-2003 Nitrogen gas generator with oxygen analyzer 5183-2004 Fitting set for single-instrument generator installation: 5180-4145 1/4-inch male NPT to 1/4-inch Swagelok, brass (2/package) Maintenance pre-filter cartridge kit for 5183-2003 or 5183-2004 5183-2014

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36 Agilent G6230AA/G6224AA TOF Site Preparation Guide



www.agilent.com

In This Book

This book contains information to prepare your site for the Agilent 6224/6230 Accurate Mass TOF LC/MS system components.

Agilent Technologies, Inc. 2009

Printed in USA 08/09 Second edition, August 2009

G2597-90034

Agilent G6230AA/G6224AA TOF Site Preparation Guide An Application Kit for the Screening of Samples for Analytes of Forensic Toxicological Interest using TOF or Q-TOF LC/MS with a Personal Forensic Toxicology Database

Application Note Forensic Toxicology

Authors Abstract

Peter Stone and Jerry Zweigenbaum A Forensic Toxicological screening application kit has been developed for use with Agilent Technologies, Inc. the Agilent TOF and Q-TOF Mass Spectrometers which contains an accurate mass 5301 Stevens Creek Blvd. database with a content of around 6700 analytes. The aim of the MassHunter Santa Clara, CA 95051 Personal Forensic Toxicology Database Kit is to provide a user with a sufficient USA starting point for the analysis of samples for which the ability to detect and identify from a large array of forensic toxicological analytes is necessary. The combined system allows the user to create custom databases containing retention times of compounds of interest for smaller and more specific suites of analytes according to specific requirements. A test mix containing analytes of forensic interest, to demonstrate the functionality of the MassHunter Personal Forensic Toxicology Database Kit, together with an example of a general screening method for common drugs of abuse is provided. Introduction Experimental The application of high definition accurate mass spectrome- The analysis results outlined in this application note were ters, such as time-of-flight (TOF) and quadrupole time-of-flight obtained using an Agilent 6230 Time-of-Flight LC/MS coupled (Q-TOF), to screening, discovery and confirmation in the areas to an Agilent 1200 SL Series LC system. The LC system con- of forensic toxicology has become more desirable given the sisted of a binary pump (G1312B), vacuum degasser indiscriminant and non-targeted nature of their full spec-tral (G1379B), automatic liquid sampler (G1367D), thermostatted data capture. Indeed, given the highly accurate and sensi-tive column compartment (G1316B) and MassHunter Workstation mass measurement of modern TOF and Q-TOF instru-ments equipped with the (G6855AA) MassHunter Personal Forensic (sub 2-ppm mass accuracy, pg on-column sensitivity and high Toxicology Database Kit. resolution) in combination with powerful software data mining tools, post acquisition screening techniques are easier to Sample preparation perform reliably with a higher number of analytes in one An ampoule from the LC/MS Forensic Toxicology Test Mix analytical method. The lists of potential toxins are large and (p/n 5190-0470) which is included in the MassHunter typically depend on the area of analytical focus. Personal Forensic Toxicology Database Kit [G6855AA] was opened and 10 µL of the 1 µg/mL (1 ppm) solution was Accurate single-stage mass spectrometry (MS) mass mea- diluted to a concentration of 100 ng/mL (100 ppb) using 990 surements identify monoisotopic adducts to a high confirma- µL of pure LC/MS grade methanol to create a clean solvent tory degree, and databases can be built to accommodate vari- standard for method checkout purposes. ous suites of forensic toxicological analytes of interest. They are obtained from both TOF and Q-TOF LC/MS instru-ments. In Table 1 outlines the composition of the LC/MS Forensic contrast LC/MS/MS with a triple quadrupole MS in its most Toxicology Test Mix (p/n 5190-0470) which is intended to sensitive mode, multi-reaction monitoring (MRM), provides cover a wide and representative range of forensic analyte targeted screening and confirmation only [1]. classes. Table 1. LC/MS Forensic Toxicology Test Mix Components (1 µg/mL) This application note describes the Agilent MassHunter Compound name Formula Mass Personal Forensic Toxicology Database Kit for Forensic 3,4-Methylendioxyamphetamine (MDA) C H NO 179.09463 Toxicological Screening and Identification which contains the 10 13 2 accurate mass (AM) details for around 6700 analytes of foren- 3,4-Methylenedioxyethamphetamine (MDEA) C12H17NO2 207.12593 sic toxicology interest. The content was gathered upon advice Alprazolam C17H13ClN4 308.08287 from many leading institutions and knowledge bases world- Clonazepam C15H10ClN3O3 315.04107 wide and contains information such as common names, Cocaine C H NO 303.14706 monoisotopic mass, compound formulas, CAS & Chemspider 17 21 4 Codeine C H NO 299.15214 IDs, chemical structure and in most cases the IUPAC nomen- 18 21 3 delta9-Tetrahydrocannabinol (THC) C H O 314.22458 clature. In addition to accurate mass, the ability to add reten- 21 30 2 Diazepam C H ClN O 284.07164 tion time for a chromatographic method to every analyte for 16 13 2 Heroin C H NO 369.15762 extra search confirmation is a built-in functionality of the 21 23 5 MassHunter Personal Compound and Library (PCDL) program Hydrocodone C18H21NO3 299.15214 interfaces. This allows accurate mass retention time (AMRT) Lorazepam C15H10Cl2N2O2 320.01193 data mining routines. Furthermore, an analyst can use the Meperidine (Pethidine) C15H21NO2 247.15723 database content 'as is' for non-targeted screening or create Methadone C H NO 309.20926 smaller custom and more targeted databases from the read- 21 27 Methamphetamine C H N 149.12045 only supplied database. Custom databases can be edited by 10 15 Methylendioxymethamphetamine (MDMA) C H NO 193.11028 changing entries, adding, and deleting entries and semi-auto- 11 15 2 Nitrazepam C H N O 281.08004 matically updating retention times for particular analytes and 15 11 3 3 methods. [2] The analyst can create as many custom data- Oxazepam C15H11ClN2O2 286.05091 bases with LC-dependent retention times as needed. Oxycodone C18H21NO4 315.14706 Phencyclidine (PCP) C H N 243.1987 This application note describes the typical use of the 17 25 Phentermine C H N 149.12045 MassHunter Personal Forensic Toxicology Database Kit 10 15 through a few analytical screening work flow examples. Proadifen C23H31NO2 353.23548

Strychnine C21H22N2O2 334.16813

Temazepam C16H13ClN2O2 300.06656

Trazodone C19H22ClN5O 371.15129

Verapamil C27H38N2O4 454.28316

2 Reagents and chemicals MS acquisition method parameters Reference ion mass enabled 121.050873, 922.009798 Burdick & Jackson LC/MS grade acetonitrile together with de-ionized water (locally produced 18.1 MW) were used for Acquisition mode MS1 mobile phases. Buffers were freshly prepared using a high Minimum mass value 50 m/z purity source of formic acid and ammonium formate. Maximum mass value 1,050 m/z Scan rate 3 Hz Instrument settings and MS acquisition method parameters All other instrument operating parameters were taken care of by Agilent's autotune functionality and subsequent mass LC conditions calibration using standard settings. Column Agilent ZORBAX Eclipse Plus C18, 2.1 mm × 100 mm, 1.8 µm (p/n 959764-902) Results and Discussion Column temperature 60 °C

Mobile phase A) 5 mM NH4 formate/0.01% formic acid in water B) 0.01% formic acid in acetonitrile Fast and easy start up with Agilent LC/MS Flow rate 0.5 mL/min Forensic Toxicology Test Mix Gradient program Time (min) %A %B Flow rate (mL/min) The LC/MS ForensicToxicology Test Mix (p/n 5190-0470) is 0 90 10 0.5 included in the MassHunter Personal Forensic Toxicology 0.5 85 15 0.5 Database Kit (G6855AA) to rapidly implement the method and 3.0 50 50 0.5 verify that acquisition and data analysis methodology is 4.0 5 95 0.5 6.0 5 95 0.5 correctly set up. The LC/MS Forensic Toxicology Test Mix contains a representative range of components from 25 Injection volume 1 µL (with 5 second needle wash in flushport) forensic analyte classes. Analysis time 6.0 minutes (see Table 1). MS screening depends on accurate mass Post Time 2.0 minutes results from the TOF or Q-TOF. Therefore, the use of appropri- Overall cycle time 8.0 minutes ate reference ions as outlined in the Experimental conditions section obtains the most accurate results. Agilent 6230 TOF MS conditions Source conditions ×106 Cpd 10: Codeine: +ESI ECC Scan Frag=150.0V Tox_Std_SeqA-r028.d Electrospray AP-ESI (using Agilent Jet Stream Technology) 1.4 Positive ionization polarity 1.3 Sheath gas temperature and flow 380 °C, 12 L/min 1.2 Nozzle voltage 500 V 1.1 Drying gas temperature and flow 320 °C, 8 L/min 1 0.9 Nebulizer gas pressure 27 psi 1.169 Capillary voltage 3,750 V 0.8 Cpd 10: Codeine Fragmentor voltage 150 V 0.7 Electrospray AP-ESI 0.6 Positive ionization polarity 0.5 Drying gas temperature and flow 350 °C, 12 L/min 0.4

Nebulizer gas pressure 30 psi 0.3

Capillary voltage 2,000 V 0.2

Fragmentor voltage 150 V 0.1

0 123456 Counts vs. Acquisition Time (min)

Figure 1. Extracted compound chromatogram of LC/MS Forensic Toxicology Test Mix.

3 In compliance with the methodology outlined in the experi- Personal Compound Database and Library (PCDL) mental section, a 1-µL injection of the 100 ng/mL LC/MS Software interface Forensic Toxicology Test Mix equates to a 100 pg on-column Outline injection amount. Figure 1 shows an overlay of the expected An open database dialog box appears after invoking the PCDL extracted compound chromatograms for the LC/MS Forensic interface from the desktop icon. It is best to choose the pre- Toxicology Test Mix. A standard method is included for TOF installed Forensic.cdb from the MassHunter\database direc- and Q-TOF as part of the MassHunter Personal Forensic tory. Figure 2 illustrates the single search view of the software Toxicology Database Kit. These can be loaded so that all interface. The screen shows a list of search results for conditions are correct and the user can reproduce the analysis. amphetamine. There are seven views available to the user, however, for the scope of this application note, only the first These methods are acquisition only methods and correspond four (tabs to the left) that are directly applicable to AMRT to the instrument configuration as outlined in the experimen- functionality will be described. These views are switched on tal section of this application note. Appropriate settings must this flat user interface by clicking the appropriate tab: Single be manually input if a different instrument configuration is Search, Batch Search, Batch Summary, or Edit Compounds. used. Similar results will demonstrate that the system is working properly.

Figure 2 Single Manual Search view of the PCDL software interface.

4 Any field or combination of fields in the upper portion of the Workflow A. Manual (Single Mass Search) Single Search tab (Figure 2.) can be used to manually search the loaded database. Table 2 lists all available search fields Using PCDL Program from the PCDL single search view. The powerful search algo- Single search would normally be used manually by obtaining rithm also handles partial names (for example, 'amph' will a measured mass from a measured or observed spectrum in return all database entries containing this letter string.) MassHunter Qualitative Analysis program and typing it in to the mass search field. Figure 3 illustrates this manual applica- Note: To view the entire contents of the loaded database, a tion of the MassHunter Qualitative Analysis program and single search invoked with all empty search fields will allow PCDL single search capability for observed masses. the user to display the entire database content. In this example, a compound peak was identified in MassHunter Qualitative Analysis program from positive polar- Table 2. All Available Search Fields for PCDL Single Search ity TOF data, the spectrum was extracted, and the observed Search fields available mass of 244.205770 m/z was searched against the PCDL (Single Search view) Value database (including cations) for [M+H]+ adducts using a Mass Measured mass (m/z) mass tolerance of 10 ppm. Retention time (minutes) Formula Empirical Formula The search returns an accurate mass match with phencyclidine (PCP) and with a mass deviation (or delta mass) of Name Common name of compound (or part thereof) 0.85 ppm between the measured and theoretical database Notes Compound class or description values. IUPAC IUPAC or commonly recognized compound name CAS Unique CAS number More detailed information of single search capability can be ChemSpider Unique ChemSpider ID found in Agilent G6855AA MassHunter Personal Forensic Toxicology Database and Kit Quick Start Guides [3,4] .

×105 +ESI Scan (2.838-2.997 min, 30 scans) Frag=150.0V Tox_Std_1ppm… 244.205770 1.6 1.4 1.2 1 0.8 0.6

0.4 245.208943 0.2 246.211774 0 243.5 244 244.5 245 245.5 246 246.5 Counts vs. Mass-to-Charge (m/z) Figure 3. Manual search of observed mass.

5 Figure 4A. Manual Search of observed mass using Agilent MassHunter Qualitative Analysis program. Single manual search of database using Agilent MassHunter Qualitative Analysis program To obtain a seamless single spectral peak database search via MassHunter Qualitative Analysis program, the database must be specified in the qualitative analysis method editor. Compatible software versions are B.03.01 or higher. Figures 4A through 4D illustrate the settings used for this example.

Figure 4a shows the typical MassHunter Qualitative Analysis program view containing the chromatographic peak in question together with its manually extracted spectrum. On the left side of the screenshot, the Identify Compounds method explorer options have been expanded and the Search Database method editor was selected. In the method editor, the required AMRT database was specified as forensic.cdb.

Figure 4B shows the mass tolerance window and the search criteria that can be selected, such as mass only or mass with retention time. Figure 4B. Manual search criteria settings.

6 Figure 4C illustrates more adduct and charge state options required for the database search.

Right-click in the spectrum window and a shortcut menu appears against the specified AMRT database (Figure 4A.) This menu has various options including Search database for spectrum peaks. Selection of this option automatically invokes the database search. In Figure 4D the spectrum peak has been identified as PCP, with 0.87 ppm mass deviation and a spectral combined score of 99.36 out of 100, indicating extra confirmation of identity.

To calculate this score, three distinct score components were considered: Mass Match, Abundance Match, and Spacing Match with values of 99.61, 98.61, and 99.79, respectively. These are individually displayed in Figure 4D.

For trustworthy results, the software scores the database matches based on the similarity of each of the isotopic masses (Mass Match), isotope ratios (Abund Match), isotope spacing (Spacing Match), and optionally the retention time (RT Match). Figure 4C. Manual Search Adduct selection.

Scoring based on Monoisotopic mass (varies in ppm)

Isotope abundance (varies in %)

Isotope spacing (varies in ppm)

Figure 4D. Manual database search results using Agilent MassHunter Qualitative Analysis program.

7 Isotope spacing is another important component of the scoring algorithm. The mass spacing from the M to the M+1 and M+2 isotopes can be measured with low-ppm accuracy. Any small mass shifts affect all isotopes equally, so this measurement is independent of overall mass axis shifts. This is outlined graphically in Figure 4D.

In this example, a single AMRT database result of phencyclidine (PCP) was returned, together with its structure which is optionally overlaid on the peak spectrum as shown in Figure 4D and can be displayed if selected in the reporting options.

More detailed information about MassHunter Qualitative analysis program database searching can be found in the MassHunter Qualitative Analysis Program Help Files or user guides [5]. Workflow B. Data mining using Molecular Feature Extractor (MFE) Batch PCDL searches (tabs 2 and 3) are designed for data- Figure 5A. MFE extraction parameters. base searching and identification using an accurate mass list created from an automated data mining algorithm such as the Agilent Molecular feature extractor (MFE.) Such algorithms are extremely powerful, especially with complex data derived from difficult sample matrices, such as blood extracts. For the remainder of this application note, only batch searches invoked from inside the MassHunter Qualitative Analysis program interface will be outlined and described. For information on how to perform batch searches within the PCDL interface, please refer to the PCD application note [2].

Data mining algorithms such as MFE automatically search and mine complex sets of single-stage MS data to determine and distinguish most likely and real compound peaks from continuous background interferences. Combinations of adducts can be selected as part of the compound identification protocol to provide added assurance of compound validity.

Other data mining algorithms such as find by MS/MS and find by Targeted MS/MS are integral options included as part Figure 5B. MFE ion species setup. of the MassHunter Qualitative Analysis program software. The algorithms are dependent on the mode of operation and editor was opened under the method explorer in the Find nature of the instrument being used. Find by Formula com- Compounds section (Figures 5A and B). pound search routines are described in the Workflow C section of this application note. A very aggressive setting of absolute peak height threshold (>500 counts) was used in this example (Figure 5A), together For illustrative purposes, the LC/MS Forensic Toxicology Test with the small molecules algorithm (chromatographic) which Mix was analyzed under the conditions outlined in the yielded over 3,000 possible compound hits. By raising this experimental section. The data file was loaded into threshold amount, less abundant analytes may remain unde- MassHunter Qualitative Analysis program. The Find by tected. Conversely with a higher threshold the number of Molecular Feature method potential false positives are greatly reduced. Only [M+H]+

8 Figure 6. MFE compound database search settings. adducts were searched in this instance, however, further confidence could have been sought (Figure 5B) by choosing + + additional adducts such as Na and NH4 . No compound, mass filters or mass defect filters were specified for this search and a maximum charge state of 1 was specified in the MFE method setup. The next step after MFE search was to specify the forensic toxicology AMRT database (Figure 6) in the identify compound/search database method editor, highlight all of the MFE-found compounds and search each compound against its content. A mass and retention time (RT) match was specified, since RT database values had already been pre-determined by analyzing individual standards and inserted into a customized compound database.

9 Figure 7 illustrates the results obtained from the MFE operation invoked by pressing the green process button highlighted in the title bar of the MFE method editor (Figure 6).

Figure 7. MFE compound database search results using Agilent MassHunter Qualitative Analysis program.

10 These results are detailed in Table 3 and show that all Isobaric compounds such as codeine/hydrocodone and 25 compounds of the LC/MS Forensic Toxicology Test Mix methamphetamine/phentermine were also correctly identi- were identified for this sample injection. This confirms that fied and distinguished automatically, by using the retention the data analysis settings for the find and identify steps are capability of the PCDL database and by inputting the pre- appropri-ate for the identification process. Many of the 3,000+ determined retention time of each analyte for this chromato- com-pounds identified by MFE did not find any PCDL matches graphic methodology as outlined in the Agilent G6855AA as expected and the data analysis option of excluding MassHunter Personal Forensic Toxicology Database Quick non-positives was used to report only the database hits. Start Guide [3].

Table 3. MFE Compound and Database Search Results

RT Diff Mass Diff Score Name RT RT (DB) (DB) Mass (DB) (DB, ppm) Formula (DB) (DB)

Verapamil 3.574 3.577 0.003 454.2833 454.2832 -0.31 C27 H38 N2 O4 98.43

Trazodone 2.84 2.824 -0.016 371.1516 371.1513 -0.81 C19 H22 Cl N5 O 59.25

Temazepam 3.94 3.946 0.006 300.067 300.0666 -1.62 C16 H13 Cl N2 O2 97.01

Strychnine 1.788 1.769 -0.019 334.1684 334.1681 -0.77 C21 H22 N2 O2 98.67

Proadifen 4.116 4.121 0.005 353.2355 353.2355 -0.18 C23 H31 N O2 98.05

Phentermine 1.77 1.75 -0.02 149.1199 149.1205 3.78 C10 H15 N 89.91

Phencyclidine (PCP) 2.931 2.901 -0.03 243.199 243.1987 -1.32 C17 H25 N 72.24

Oxycodone 1.434 1.423 -0.011 315.1475 315.1471 -1.44 C18 H21 N O4 91.16

Oxazepam 3.524 3.528 0.004 286.0511 286.0509 -0.71 C15 H11 Cl N2 O2 98.37

Nitrazepam 3.535 3.544 0.009 281.0804 281.08 -1.34 C15 H11 N3 O3 99.2

Methylendioxymethamphetamine 1.625 1.621 -0.004 193.1108 193.1103 -2.77 C11 H15 N O2 79.54 (MDMA)

Methamphetamine 1.606 1.593 -0.013 149.1197 149.1205 4.82 C10 H15 N 81.88

Methadone 3.638 3.638 0 309.2094 309.2093 -0.61 C21 H27 N O 99.67

Meperidine (Pethidine) 2.477 2.456 -0.021 247.1577 247.1572 -1.7 C15 H21 N O2 97.91

Lorazepam 3.616 3.621 0.005 320.012 320.0119 -0.19 C15 H10 Cl2 N2 O2 98.27

Hydrocodone 1.575 1.56 -0.015 299.1525 299.1521 -1.2 C18 H21 N O3 85.2

Heroin 2.322 2.297 -0.025 369.1579 369.1576 -0.63 C21 H23 N O5 98.97

Diazepam 4.272 4.275 0.003 284.072 284.0716 -1.36 C16 H13 Cl N2 O 58.97 delta9-Tetrahydrocannabinol (THC) 5.275 5.292 0.017 314.2243 314.2246 0.94 C21 H30 O2 94.83

Codeine 1.169 1.16 -0.009 299.1524 299.1521 -0.72 C18 H21 N O3 72.49

Cocaine 2.44 2.418 -0.022 303.1475 303.1471 -1.29 C17 H21 N O4 98.03

Clonazepam 3.625 3.638 0.013 315.0412 315.0411 -0.42 C15 H10 Cl N3 O3 98.72

Alprazolam 3.726 3.726 0 308.083 308.0829 -0.33 C17 H13 Cl N4 96.77

3,4-Methylenedioxyethamphetamine 1.862 1.846 -0.016 207.1263 207.1259 -1.8 C12 H17 N O2 97.4 (MDEA)

3,4-Methylendioxyamphetamine 1.474 1.473 -0.001 179.095 179.0946 -2.23 C10 H13 N O2 86.15 (MDA)

11 Customized databases with user-added retention times Customizing and updating PCDL AMRT compound data is One of the benefits of the Agilent Personal Forensic accomplished by using tab 4 (from left) of the PCDL program Toxicology Database is that it can be saved to a user cus- interface. This is shown in Figure 8, where the options of tomized form. To create a read-write customizable database Add New, Save as New, Update Selected, and the user selects New Database from the PCDL File menu. The Delete Selected are clearly present. When Allow Editing is PCDL program then allows selection of an existing database activated from the Database/Library pull-down menu, any of and the naming of a new database. A description can also be the displayed information fields in the users' custom given. When Create is selected, the database with the new database can be changed, added to, or deleted. Furthermore, name contains all the entries of the selected database. In this the ability to insert *.mol molecular diagrams to any new way multiple custom or smaller, more targeted databases can database entry is possible from the Edit Compounds tab. be created depending on the analytes of interest. A technical note on the Pesticide PCD [2] shows how users can run standards with unique chromatographic conditions and easily update or insert retention times in their custom database.

Figure 8. Edit Compounds PCDL interface tab.

12 Workflow C. Data mining using 'Find by Formula' spacing in a combined score (shown) together with retention (FBF) time. The DA method editor settings used for this FBF analysis are shown in Figure 10, where Tox_std_01.cdb was a The Find by Formula data-mining algorithm of the custom PCDL-format database. MassHunter Qualitative Analysis program uses a pre-defined empirical formula (or list of formulae) to search TOF and When reporting the results, FBF assesses the chromato- Q-TOF (MS) data files for evidence that peaks may be present. graphic peak shape and isotopic match scores and returns The PCDL-format databases can also be specified as the list the best match, even if there are several peaks displayed in of empirical formulae. Depending on the size and content of the extracted compound chromatogram of similar mass. the database, FBF can take slightly longer than the MFE + + + approach. However, FBF is highly accurate and sensitive Additional adducts [M+Na] , [M+NH4] and [2M+H] were especially at very low analyte concentration levels. used during this FBF data screen. The extra information is displayed in the spectrum view and results table to provide Figure 9 illustrates the results screen displayed after a Find by added confirmatory evidence. Figure 9 shows the Temazepam Formula search has been undertaken using the LC/MS spectrum which displays both [M+H]+ and [M+Na]+ adducts. Forensic Toxicology Test Mix data file. All 25 compounds were matched with accurate mass, abundance and isotopic

Figure 9. Find By Formula Database search results, Agilent MassHunter Qualitative Analysis program.

13 Figure 10. Find By Formula Database search - Method editor settings.

More in-depth information can be obtained from MassHunter Qualitative Analysis program Help files or Agilent MassHunter Workstation Software Qualitative Analysis Familiarization Guide [5].

Reporting

Manual, MFE and FBF database searching all use the identical method of compound reporting options in the MassHunter Qualitative Analysis program software interface. Figure 11 details the reporting options which are based upon the standard compound report template CompoundReportWithIdentificationHits.xlsx. Under the General section of the method explorer, the 'Common report- Figure 11. Common compound reporting options for Manual/MFE/FBF ing options' link opens the corresponding method editor PCDL Searches. pane, shown on the left side of Figure 11. MassHunter Qualitative Analysis program treats search algorithm data and database searches as compound-centric data. Therefore, to report the results the appropriate compound report template must be chosen. In this example, the correct report template is displayed. 14 More specific content can then be specified by choosing the information required for the Forensic Toxicology screen report using the Compound Report options of the method editor (shown on the right in Figure 11).

Decisions about the report content are decided here. For example, if the check box for Exclude Details for Unidentified Compounds is activated, then only positive PCDL identifications will be reported. The option to report compound extracted chromatograms, individual MS spectra, or summary results and individual compound tables is also determined from the compound report method editor.

Once all the correct settings have been achieved for the reporting of results, the green button (circled in Figure 12) activates the printing dialogue window which gives various options for directing the output of the data file results. The user can choose to send results directly to a specified printer or save the results in excel format or public distribution format (pdf). Alternatively, the results report can be processed by choosing the Print Compound Report option from the drop-down File menu.

Figure 12. Compound Reporting for Manual/MFE/FBF PCDL Searches.

15 Figure 13 illustrates a typical report summary front page for the LC/MS Forensic Toxicology Test Mix.

Figure 13. Output Report from MFE/Database search.

16 Worklist Automation In this example, a list of automatic data analysis steps are defined in order of operation, as they would be undertaken Once the analyst or operator has decided on the correct set- manually. tings for all aspects of the data mining routines, the PCDL search options and reporting options (outlined in this applica- First, the sample data file is loaded, and all previous results (if tion note) can be saved to one convenient data analysis any) are cleared. Next, the Find by MFE routine according to method. This method can be used for repetitive and consis- the saved DA method setup is performed with the compound tent data manipulation from week to week. This is achieved results searched against the PCDL database specified in the by choosing the Save As option from the drop-down Method DA method. Finally, any results are automatically sent to a menu in the MassHunter Qualitative Analysis program inter- final report, the format of which has been determined and face. This method will then open as the default DA method also saved to the DA method. when the MassHunter Qualitative Analysis program is started Two further steps must be performed to run such a worklist until another DA method is saved or loaded. automation routine automatically during sample data An added advantage to saving reprocessing options is the acquisition. Worklist Automation functionality built into the MassHunter First, the DA analysis method and the Worklist Automation Qualitative Analysis program. Figure 14 outlines the setup of routine must be saved into the acquisition method by using Worklist automation and specifically addresses a routine that the Save As option from the Method menu and selecting the would automatically interrogate a data file using MFE and MassHunter acquisition method name. Once OK is selected, PCDL database search followed by reporting of results to the the data analysis method becomes an integral part of the specified printer or data file location. Acquisition method.

Figure 14. Worklist automation method setup.

17 Finally, to automatically perform Worklist Data Analysis during References data acquisition, the Worklist Run Parameters window must be opened from the Worklist menu of MassHunter Acquisition 1. Multi-Residue Pesticide Analysis with Dynamic Multiple software. Figure 14 shows a screen capture of this window Reaction Monitoring and Triple Quadrupole LC/MS/MS, with the settings highlighted so that the DA routine will oper- Agilent Technologies Application Note, publication ate Parts of method to Run - Both Acquisition and DA. The number 5990-4253EN. data analysis has the option to be run Synchronously or 2. Pesticide Personal Compound Database for Screening Asyncronously. and Identification, Agilent Technologies Technical Note, publication number 5990-3976EN. Conclusions 3. Agilent Personal ForensicToxicology Database Quick Start The Agilent MassHunter Personal Forensic Toxicology Guide, Agilent Technologies, publication number G6855- Database Kit has been developed to provide comprehensive 90003. screening of samples for both targeted and non-targeted 4. Agilent G6855AA MassHunter Personal Forensic approaches. The database includes accurate mass data for Toxicology Database Kit Quick Start Guide, around 6,700 compounds of potential interest and gives the Agilent Technologies, publication number 5990-4264EN user flexibility in its use. 5. Agilent MassHunter Workstation Software Qualitative The MassHunter Personal Forensic Toxicology Database Kit Analysis Familiarization Guide, Agilent Technologies, offers: publication number G3335-90060.

• Fast and easy startup of complex analyses • A comprehensive database of around 6,700 compounds including • Chemical structures, formulas and exact masses • Direct Chemical Internet links to PUBCHEM and ChemSpider • IUPAC names • The ability to create MS/MS spectral libraries • Complete customization with additions/deletions of retention time for chromatographic conditions developed by the user • Results can be searched from within the PCDL software interface or directly from the MassHunter Qualitative www.agilent.com/chem Analysis program. For Forensic Use. • Results can be data-mined with powerful searching tools, such as the Molecular Feature Extractor and Find by This information is subject to change without notice. Formula © Agilent Technologies, Inc., 2009, 2016 • Searches of the database can be partially or completely Printed in the USA March 17, 2016 automated using MassHunter Qualitative Analysis pro- 5990-4252EN gram and the MassHunter Acquisition Worklist B. Time of Flight Mass Spectrometer (TOF) 1. Equipped with a high stability TOF mass analyzer

Yes, we comply. We propose Agilent 6230 TOF MS. More details about this system are included in the attached sales brochure. This system is known for its high stability because of its low thermal expansion flight tube design. To minimize mass error, which is introduced by room temperature variations, the flight tube material has to contain low thermal expansion rate material. The inner flight tube must be separated from outer flight tube using low vacuum to provide extra temperature stabilization for consistent mass accuracy. This system has ability to achieve steady mass accuracy without adding expensive temperature control systems in the operating room.

AGILENT 6230 TIME OF FLIGHT MASS SPECTROMETER

Agilent 6230 TOF MS comes with a proprietary ESI source called Agilent JetStream® (AJS) source whose design includes a single-bend, orthogonal ESI spray. Further details about Agilent JetStream ESI source is given below. All Agilent ion source designs include sprayers that are positioned orthogonal to the inlet of the mass spectrometer. The orthogonal spray helps to improve the signal/noise and minimize maintenance by keeping the system clean. The single- bend design minimizes ion losses for maximum sensitivity.

Jet Stream Ion Source Technology

Agilent Jet Stream thermal gradient focusing technology was developed to significantly enhance sensitivity in ESI-MS by improving the desolvation and spatial focusing of ions. Superheated nitrogen sheath gas confines the nebulizer spray to more effectively dry ions and concentrate them in a thermal confinement zone. Desolvation reduces noise. Full confinement of the spray by the superheated nitrogen gas eliminates sample recirculation and reduces peak tailing. Improved ion production results in higher MS and MS/MS signal intensities and improved signal-to-noise ratios. On average, a 5 to 10-fold improvement in MS and MS/MS sensitivity is realized by using Agilent Jet Stream technology versus standard ESI ionization. a. Mass measurement accuracy of <2.5 ppm

Mass accuracy of Agilent 6230 TOF MS is < 1 ppm exceeds the mass accuracy requirements. Please see the Data Specifications Sheet for details. b. Utilizes lock mass solutions for mass scale calibration accuracy of <1 ppm over a mass range of 50-2000 m/z

Yes we comply

2. Must be capable of full scan with minimum mass ranges of: a. Positive ion mode: 50-2000 m/z or 400-7000 m/z b. Negative ion mode: 50-2000 m/z or 400-5000 m/z Yes we comply.

3. The system is able to collect: Full Scan Mass Spectra (MS) Or Simultaneous full scan MS and fragment ion MS (Full Scan with Fragmentation)

Yes we comply. Agilent 6230 TOF MS comes with All Ions feature which is full scan with fragmentation.

4. The TOF has a pre-optimized ionization source which requires no physical user- adjustment for consistent performance.

Yes, we comply

5. The TOF is able to self-diagnose faults. If a fault occurs with the instrument, the software needs to display information that provides step-by-step guidance to fix the fault.

Yes, we comply. The System Logbook will display any faults that occur in the system. The built-in Help tab in MassHunter Acquisition, in addition to the MassHunter Resources program contains information to diagnose instrument faults.

6. Routine maintenance can be performed without venting the TOF or replacing the sample cone assembly or equivalent.

Yes, we comply. Daily and weekly source cleaning procedures, in addition to Nebulizer cleaning and needle replacement do NOT require venting the TOF. As mentioned above in Sec B, Item 1. all Agilent ion source designs include sprayers that are positioned orthogonal to the inlet of the mass spectrometer. The orthogonal spray not only helps to improve the signal/noise but also minimize maintenance by keeping the system clean. The single-bend design minimizes ion losses for maximum sensitivity.

7. The TOF mass analyzer has a small foot print no bigger than 80 (H) x 40 (W) x 80 (D) cm (H W x D).

Footprint details of Agilent 6230 TOF MS are given below:

8. The TOF can be connected to a single-phase mains electricity supply that has a nominal voltage within the range 100 to 127 Volts, without the need for much laboratory preparation including an additional transformer within the laboratory.

Agilent 6230 TOF MS would need 200 VAC for which a single 50 Hx power outlet is needed. See detailed power requirements below:

9. System already demonstrated to be capable of the analysis of seized drug unknown samples. Please see the attached Application Note: “An Application Kit for the Screening of Samples for Analytes of Forensic Toxicological Interest using TOF or Q-TOF LC/MS with a Personal Forensic Toxicology Database” – Authors Pete Stone and Jerry Zweigenbaum, Application Scientists, Agilent Technologies, Inc. C. Instrument Software Requirements 1. Must include reliable processing and instrument control software for setting up sequence/run tables, tuning, method configurations, real-time system status, and diagnostics. a. Automated workflow for routine analysis data processing b. Customizable, automated reporting c. Software is capable of communicating with common Laboratory Information Management Systems (LIMS) for data analysis and reporting functionality.

Yes, we comply. All instrument parameters for routine operations, diagnostic procedures for all performances are under Agilent MassHunter software suite. Agilent MassHunter Acquisition User Interface is shown below:

MassHunter User Interface (above): Eight windows shown above provide the tools to set up acquisition methods, run samples interactively or automatically, monitor instrument status, monitor runs and tune the instrument. Actuals window shows real-time status of the various parameters of the instrument.

2. Must include spectral interpretation and classification data for the identification of unknowns.

The system comes with Agilent MassHunter software suite which includes – MassHunter Qualitative analysis and MassHunter Quantitative analysis for spectral interpretation and quantitation applications.

3. Software must include a mass library of pharmaceutical and controlled substances for forensic purposes. a. Software must allow for building custom, in-house libraries.

Agilent Forensic Toxicology Personal Compound Database and Library is included with this system. This database is developed with highest Agilent quality and reliability for forensic applications. We also provide guidance for quick start. The library can also be expanded for increased screening scope, and the software supports easy addition of new compounds and building custom in-house libraries.

4. Software must include drug discovery tools to access chemical databases to identify unknown compounds from analysis.

Yes, Masshunter Qualitative analysis includes easy access to other chemical databases to identify unknown compounds from analysis.

5. PC system capable of integration into Sheriff Network (minimum Windows 10, 16 GB memory, modern CPU processor) a. Hard drive storage capacity for modern TOF data storage (2 terabytes or more) Yes, we comply. HP Z4 G4 PC is used for Agilent 6230 TOF MassHunter PC. Following are detailed specifications of this computer: 1. Operating System: Windows 10 Pro 64-bit 2. PROCESSOR: Intel Xeon W-2133 (3.6 GHz, 8.25 MB cache, 6 cores) CHIPSET: Intel C422 3. MEMORY: 16GB (2x8GB) DDR4 2666 DIMM ECC Registered Memory 4. STORAGE: 4x 500GB SATA 7200 rpm 6Gb/s 3.5" HDD 5. OPTICAL DRIVE: HP USB External DVDRW Drive (F2B56AA) 6. COMMUNICATIONS: Integrated Intel I219 and I210 PCIe GbE; HP Serial Port Adapter; Intel® X550-T2 10GbE Dual Port NIC 7. A mouse, mouse pad and a keyboard included. 8. A 24-inch flat panel LCD monitor included 9. Includes 3 Year Warranty from HP through Agilent. This means that calls for support are taken by Agilent on the 1-800 number or other means and routed to HP for service delivery. 10. Cable assembly will be provided for connecting all components 11. GRAPHICS/DISPLAY: AMD FirePro W2100 2GB GFX 2x Display Port1.2 connectors CI. Nitrogen Generator 1. Must include multi-stage purification system capable of nitrogen gas purity of 99.5%. 2. Generator capable of variable pressure up to 116 psi with max flow rate of 35 L/min 3. Generator system must be Phthalate and Butylated hydroxytoluene (BHT) free. 4. Generator must be capable of supporting high performance LC-TOF instruments. 5. Generator must be able to utilize a 120V outlet.

Yes, we comply

CII. Consumables 1. Must include 1 pack of 50 0.2μm 25mm syringe filters.

2. Must include ten 5.75 glass Pasteur pipettes.

3. Must include two 2-mm latex pipette bulbs.

4. Must include at least four class A 10mL volumetric pipettes. Above mentioned items in CII. Items 1 – 4 are not manufactured or distributed by Agilent and hence have not been included in this bid.

5. Must contain one kit of mass detector calibration solution containing Leu- enkephalin, Caffeine, and Pentenasulfonic Acid to compensate for mass shift to due temperature changes.

The flight tube material of Agilent 6230 TOF MS has a specially designed low thermal expansion flight tube to minimize mass error, which is introduced by room temperature variation. The flight tube is made of low thermal expansion alloy material. The inner flight tube is also separated from outer flight tube using low vacuum to provide extra temperature stabilization for consistent mass accuracy. This system has ability to achieve steady mass accuracy without adding expensive temperature control systems in the operating room. Thus, mass shift due normal room temperature variations is not a big concern with this instrument.

Contents of Agilent Tune Mix which is used for tuning, calibration and sometimes also as reference compound is attached as reference.

F. Additional Requirements 1. Training a. On-site training by an applications scientist, until the needs of the department are met.

Yes, we comply. We will provide 3 days of onsite, hands-on training and method development with the instrument. Furthermore, we have also included one seat in an offsite LC/MS/MS classroom training course (user must cover travel expenses). If offsite travel is not feasible, we can also convert this course to 1 additional day of onsite time with our Applications Scientists. We generally recommend the offsite training course, as it provides a very thorough curriculum for new users. The combination of this structured course and the specific implementation delivered will provide the users with deeper foundational knowledge.

b. Training shall be provided during normal business hours Monday through Friday from 8:00 AM to 5:00 PM (San Diego Time) excluding County Holidays.

Yes, we comply

2. Installation a. Complete, professional installation of the LC-TOF system. b. The system will be configured with a method for the analysis of seized drugs.

Yes, we comply. Agilent will complete all unpacking, setup, installation and training on the system.

3. Quality Assurance a. Include a Declaration of Conformity to ensure each system component meets or exceeds performance specifications. b. System must reliably function on a daily basis with minimal downtime.

Yes, we comply

4. Maintenance Contract a. Unlimited number of support calls, responds on site within 72 hours with factory-certified engineer. Services include the following at no additional cost: i. Engineer labor and travel ii. Planned maintenance visits iii. Performance verified parts iv. One scheduled preventative maintenance visit per year v. Technical reviews using call history vi. Software updates and notifications vii. E-support online technical resources viii. Priority status for Technical Support Inquiries, response within two hours ix. Contractor shall provide written details of all service performed on the instrument x. Contractor shall provide on-site service during normal business hours Monday through Friday from 8:00 AM to 5:00 PM (San Diego Time) excluding County Holidays

Yes, we comply b. This is to be a single vendor contract.

Yes we comply c. This Contract will be in effect for no less than two years from the installation date. Yes we comply

Next Bid

Lina Kim County of San Diego Department of Purchasing and Contracting 5560 Overland Avenue, Suite 270 San Diego, CA 92123

Re: RFB 10850 due April 22, 2021

Dear Lina,

Thank you for including Waters for RFB 10850. Waters is pleased to offer the Acquity RDa system as described on quotation #22592188.

The system includes the ACQUITY I-Class Plus binary UPLC system and the RDa detector. It can achieve the method requirements in Exhibit A I A-C. A nitrogen generator that meets the requirements in Exhibit A I CI has been included in the quote as well. Waters doesn’t manufacture or sell the consumables except for item 1 and 5 in Exhibit A I CII.

In response to the terms and conditions contained in the RFB, Waters would like to propose negotiation of a mutually beneficial agreement between Waters and the County of San Diego, if the bid to which we are responding to is awarded. Please contact Alicia Shannon, per the RFB point of contact, to discuss this in further detail.

Thank you again for considering Waters for this bid. I will be happy to help with any questions or concerns regarding this proposal. I look forward to hearing from you.

Sincerely,

Jinxi Li Mass Spectrometry Specialist Waters Technologies Corporation 949.241.6227 [email protected]

34 Maple Street Milford, MA 01757-3696 U.S.A. [T] 508.478.2000 [T] 1.800.252.4752 [F] 508.872.1990 [W] www.waters.com

Mr. Chris Vance County of San Diego Sheriffs Crime Labor Dept. Sheriff Crime Laboratory 5255 Mt Etna Dr San Diego,CA,92117-6912 US

Telephone : 0545-1430 AT 858-285-6320 Email : [email protected]

Sales Proposal Please reference this Quotation when Purchase Order is issued Quotation No: 22592188 - Expiration Date: 05/14/2021

Dear Mr. Chris Vance,

Thank you for your interest in Waters! Please find the enclosed Sales Quotation for the products you inquired about. We look forward to working with you and your team for all of your laboratory needs.

To place an order for products and services on this quotation, you may send your hard copy purchase order via email to [email protected]

You may also contact Waters Sales Support to place your order via telephone at 800-252-4752 Ext.8023, fax your purchase order to 508-482-8532 or 508-482-8834.

If you have any questions regarding this quotation, please contact your local Account Representative: Jinxi Li. Jinxi may be reached by telephone at , or via Email at [email protected], or visit us online at www.waters.com.

Waters Sales Support Tel: 800-252-4752 Ext.8023 Email: [email protected]

FOR

Waters Technologies Corporation dba Waters Corporation, 34 Maple St, Milford MA 01757 800 252 4752 This quotation is expressly conditioned upon, and subject to all terms and conditions set forth within Page: 1 / 7 Account : County of San Diego Quotation number : 22592188 Creation date : 04/13/2021 Expiration date : 05/14/2021

Sales Proposal Please reference this Quotation when Purchase Order is issued

Inco Terms: Destination (DST)

Item Product# Qty Description Unit Price Discount Net Price 1 176850054 1 Acquity™ RDa™ System 325,785.00 - 130,286.25 195,498.75 With the following configuration: 176004474 1 Acquity™ RDa™ Detector 176004707 1 RDa Reference Standards Kit 176004364 1 LC-MS Grade Water and Acetonitrile Kits 176015151 1 LC Sys Stack I-Class w/FTN/CH-A 205000726 1 CH-A Extension Kit H/I-Class for MS waters_connect 176004745 1 W/S waters_connect PC 668000273 1 MONITOR, Lenovo ThinkVision Flat Panel 176004657 1 waters_connect Workstation Software 667006223 1 waters_connect Wrk Small Molecule Option 176002866 1 Screening Solution Application Kit The Screening Solution Application Kit contains the following items: • ACQUITY UPLC BEH C18 Column, 2.1 x 100mm, 1.7 μm • DisQuE AOAC QuEChERS Kit - Pouch Format • DisQuE CEN QuEChERS Kit - Pouch Format • CH-A extension 176002908 1 Waters Screening Solution Installation Kit The Kit contains the following items: • 20 Compound Screening Standard • Ammonium Acetate 1 M pH5 • Glassware Kit: • Qty 10: 5.75 glass Pasteur Pipets • Qty 2: 2-mm latex Pipet Bulb • Qty 4: 10-mL Class A Volumet 176004662 1 Waters Multi Accurate Mass Screening Lib 740006345 1 MP1Y waters_connect Workstation 2,247.00 - 921.27 1,325.73 Installation, Training and Plans 741000518 1 ACQUITY RDa System Installation Cert Extended Warranty 176600010 1 FlexCHOICE™ Coverage for Acquity™ 115,140.00 - 23,028.00 92,112.00 RDa™ System coverage for 60 months See detail description pages for more information 2 186009339 1 Nitrogen Generator, Genius XE35, 120V 20,800.00 20,800.00 3 720002242EN 1 Implementation Kit 1.00 - 1.00 0.00

______

Waters Technologies Corporation dba Waters Corporation, 34 Maple St, Milford MA 01757 800 252 4752 This quotation is expressly conditioned upon, and subject to all terms and conditions set forth within Page: 2 / 7 Account : County of San Diego Quotation number : 22592188 Creation date : 04/13/2021 Expiration date : 05/14/2021

Sales Proposal Please reference this Quotation when Purchase Order is issued

Net Total 309,736.48 Estimated Freight 4,907.95 ______Total Quotation in USD 314,644.43 (Taxes are extra if applicable) ______

Waters Standard Terms and Conditions

Delivery: 60 Days Freight Terms: FOB Shipping Point Prepaid & Added Payment Terms: NET 30 DAYS Payment Terms Subject to Credit Review

Additional Notes:

Estimated Freight via Federal Express Saver

For Finance and Leasing Options, please contact our Waters Leasing Account Manager, Jonathan Bennett at (800) 252-4752, extension 8206.

Waters Technologies Corporation dba Waters Corporation, 34 Maple St, Milford MA 01757 800 252 4752 This quotation is expressly conditioned upon, and subject to all terms and conditions set forth within Page: 3 / 7 Account : County of San Diego Quotation number : 22592188 Creation date : 04/13/2021 Expiration date : 05/14/2021

Sales Proposal Please reference this Quotation when Purchase Order is issued

WatersTMFlexCHOICETM: mySystem Coverage WatersTM FlexCHOICETM personalized, flexible support, maintenance, and service options to meet the unique requirements of your laboratory.

- Performance Maintenance (PM) is a proactive maintenance that keeps your system operating at peak performance. One or more PM visits may be included as specified within the quote. A PM visit is a combination of Waters Quality Parts, including documented standardized protocols, delivered by a Waters Certified Field Service Specialist.

- A more detailed description of coverage and additional options are available in our Statement of Coverage, available upon request.

- Taxes may be applied at time of invoicing where applicable.

- All pricing on this quotation is subject to change, unless otherwise noted in a signed agreement. Pricing on this quotation is valid through date stated as ""Quotation Valid Until"". Payment Terms are subject to Credit Review.

- Please reference this quotation number when issuing your purchase order. Thank you for choosing Waters FlexCHOICE mySystem coverage to protect your investment. We look forward to providing the customized level of service and support that's right for your laboratory, while maintaining the performance you have come to trust with Waters

Waters Technologies Corporation dba Waters Corporation, 34 Maple St, Milford MA 01757 800 252 4752 This quotation is expressly conditioned upon, and subject to all terms and conditions set forth within Page: 4 / 7 Account : County of San Diego Quotation number : 22592188 Creation date : 04/13/2021 Expiration date : 05/14/2021

Sales Proposal Please reference this Quotation when Purchase Order is issued Detail Product Description(s) Product# Description 176015151 LC Sys Stack I-Class w/FTN/CH-A Note: This system is recommended when purchasing or supporting the ACQUITY UPLC I-Class Core System as a full LC/MS system. The LC stack is pre-assembled, pre-tested and ready for connection, to shorten system installation process for these types of integrated systems and generally more complex installs.

This ACQUITY UPLC I-Class PLUS LC Stack - Core System has integrated solvent, sample and column heating configured for binary solvent delivery and sample introduction via a Flow-Through Needle sample manager (SM-FTN-I). This system is FTN-based only.

This option is recommended currently as the CH-A based inlet of choice for any supported Waters’ mass detector. The system may be configured with UV-based UPLC optical detectors or other optical detectors and with a choice of instrument control and data management software.

205000726 CH-A Extension Kit H/I-Class for MS Extension Arm Kit

668000273 MONITOR, Lenovo ThinkVision Flat Panel 22" Flat Panel Monitor Lenovo ThinkVision L2250p - LCD display - TFT - 22" - Widescreen - 1680 x 1050 / 75 Hz - 250 cd/m2 - 1000:1 - 5 ms -0.282 mm DVI-D, VGA - business black

176600010 FlexCHOICE™ Coverage for Acquity™ RDa™ System Seamless coverage for the 60 months No. of Performance Maintenance Visits: 4 Service Level: Standard Response Time: Standard

Covered Modules: UPISMFTN+ - UPLC I SM-FTN PLUS UPBSM+ - UPLC BSM PLUS RDA - RDA UPCHA - UPLC ACTIVE COL HEAT

Waters Technologies Corporation dba Waters Corporation, 34 Maple St, Milford MA 01757 800 252 4752 This quotation is expressly conditioned upon, and subject to all terms and conditions set forth within Page: 5 / 7 Account : County of San Diego Quotation number : 22592188 Creation date : 04/13/2021 Expiration date : 05/14/2021

Sales Proposal Please reference this Quotation when Purchase Order is issued Waters General Sales Terms and Conditions

THIS TRANSACTION IS EXPRESSLY CONDITIONED UPON AND SUBJECT TO ALL OF THE FOLLOWING TERMS AND CONDITIONS: 1. Acceptance - Buyer's acceptance of the offer to purchase the products and/or services set forth on the front page made by Waters Technologies Corporation d/b/a Waters Corporation (Waters) of this quotation shall create a contract subject to and expressly limited by the terms and conditions contained on this form. Acceptance of this quotation may only be made on the exact terms and conditions set forth on this quotation; if additional or different terms are proposed by Buyer, such additional or different terms shall not become a part of the contract formed by Buyer's acceptance of the quotation. Receipt of the products sold hereunder or commencement of the services provided hereunder shall be deemed acceptance of the terms and conditions of this quotation. 2. Taxes and Payment - Any tax, duty, custom or other fee of any nature imposed upon this transaction by any federal, state or local governmental authority shall be paid by Buyer in addition to the price quoted. In the event Waters is required to prepay any such tax or fee, Buyer will reimburse Waters. Payment terms shall be net thirty (30) days after shipment and are subject to credit approval. An interest charge equal to 1 1/2% per month (18% per year) will be added to quotations outstanding beyond 30 days after shipment. In addition, Waters reserves the right, in its sole discretion, to require C.O.D. payment terms from any Buyer. Waters may also refuse to sell to any person until all prior overdue accounts are paid in full. 3. Delivery and Shipment - Delivery terms shall be F.O.B. Waters shipping point; identification of the products shall occur when they leave Waters shipping point at which time title and risk of loss shall pass to Buyer. All shipment costs shall be paid by Buyer and if prepaid by Waters the amount thereof shall be reimbursed to Waters. Waters will make reasonable commercial efforts to ship the products or provide the services hereunder in accordance with the delivery date set forth on the reverse side hereof provided, that Waters accepts no liability for any losses or for general, indirect special or consequential damages arising out of delays in delivery. 4. Warranty - The products and/or services shall be covered by the applicable Waters standard warranty, a copy of which is supplied with the products and/or services or upon request. NO OTHER WARRANTY, WHETHER EXPRESS OR IMPLIED, IS MADE WITH RESPECT TO THE PRODUCTS AND/OR SERVICES. WATERS EXPRESSLY EXCLUDES THE IMPLIED WARRANTIES OF MERCHANTABILITY AND OF FITNESS FOR A PARTICULAR PURPOSE. Any model or sample furnished to the Buyer is merely illustrative of the general types and quality of goods and does not represent that the products will conform to the model or sample. Buyer's remedies under Waters warranty shall be limited to repair or replacement of the product or component which failed to conform to Waters applicable standard warranty. WATERS SHALL NOT BE LIABLE FOR CONSEQUENTIAL, INCIDENTAL, SPECIAL OR ANY OTHER INDIRECT DAMAGES RESULTING FROM ECONOMIC LOSS OR PROPERTY DAMAGE SUSTAINED BY BUYER FROM THE USE OF ITS PRODUCTS OR SERVICES. 5. Returned Goods - Waters may, in its sole discretion, authorizeproduct returns in appropriate circumstances, subject to such conditions as Waters may specify. Any such return shall be subject to the express prior authorization of Waters and payment by Buyer of a restocking charge. No returns will be authorized after one hundred twenty (120) days following shipment to Buyer. 6. Technical Advice - Waters may, at Buyer's request furnish technical assistance, advice and information with respect to the products if and to the extent that such advice, assistance and information is conveniently available. It is expressly agreed that there is no obligation to provide such information, which is provided without charge at the Buyer's risk, and which is PROVIDED WITHOUT WARRANTY OF ANY KIND AND IS SUBJECT TO THE WARRANTY DISCLAIMERS AND LIMITATION OF LIABILITY SET FORTH IN PARAGRAPH 4. 7. Waters Right of Possession, etc. - Buyer hereby grants Waters a purchase money security interest in the goods offered by this quotation to secure the due and punctual payment of the purchase price specified in this quotation. In the event of default by Buyer in any payment due Waters, Waters shall have the right, in addition to any other remedies it may have at law or in equity, to withhold shipment, to recall goods in transit and retake the same, to repossess any goods which may be stored with Waters for Buyer's account without the necessity of Waters initiating any other proceedings. In addition, Waters shall have all of the rights and remedies of a secured party under the Massachusetts Uniform Commercial Code and may exercise all such rights and remedies in accordance therewith. Buyer shall execute such documents as Waters may request to effectuate the foregoing security interest. 8. Agents, etc. - No agent, employee or other representative has the right to modify or expand Waters standard warranty applicable to the products and/or services or to make any representations as to the products other than those set forth in the applicable user or operator's guide delivered with the products, and any such affirmation, representation or warranty, if made, should not be relied upon by Buyer and shall not form a part of contract between Waters and Buyer for the purchase of the products or services. 9. Fair Labor Standards - The products or services provided hereunder were produced and/or performed in compliance with the requirements of all sections of the Fair Labor Standards Act of 1938 as amended. 10. Equal Employment - Waters is an Equal Opportunity Employer. It does not discriminate in any phase of the employment process against any person because of race, color, creed, religion, national origin, sex, age, veteran or handicapped status. 11. Modifications, Waiver, Termination - The contract formed by Buyer's acceptance of this quotation may be modified and any breach thereunder may be waived only by a written and signed document by the party against whom enforcement thereof is sought. 12. Governing Law - The contract formed by Buyer's acceptance of this quotation shall be governed by and construed in accordance with the laws of the Commonwealth of Massachusetts, U.S.A. 13. Compliance with Laws - Buyer shall at all times comply with all applicable federal, state and local laws and regulations, including, without limitation, the provisions of the United States Export Control Laws as may be in effect for any of the products or services, and, if products or services hereunder are used in clinical applications, all applicable rules and regulations of the United States Food andDrug Administration and/or other domestic or international agencies with respect to the application of, as the case may be, Good Clinical Practices ("GCP"), Good Laboratory Practices ("GLP") or good Manufacturing Practices ("GMP"). 14. Additional Terms and Conditions - This quotation is also subject to any Waters Special Terms and Conditions applicable to the products or services offered by this quotation, which appear on the front of this quotation. Any variance from the terms and conditions of this quotation in any order or other written notification from Buyer, will be of no effect. Should Buyer order products or services through a Waters office located outside of the United States, the terms and conditions of the quotation issued by the office outside of the United States shall govern such order. 15. Arbitration - Any and all disputes or controversies arising in connection with the contract formed by Buyer's acceptance of this quotation or the sale of products and/or performance of the services shall be resolved by final and binding arbitration in Boston, Massachusetts, under the rules of the American Arbitration Association then obtaining. The arbitrators shall have no power to add to, subtract from or modify any of these terms or conditions of this contract. Any award rendered in such arbitration may be enforced by either party in either the courts of the Commonwealth of Massachusetts or in the United States District Court for the District of Massachusetts, to whose jurisdiction for such purposes Waters and Buyer each hereby irrevocably consents and submits. 16. Software - To the extent there is any software included with the products, the software is being licensed, not sold and all rights, title and interest therein shall remain with Waters. Use of the software shall be in accordance with the applicable software license delivered with the products. U.S. Government Restricted Rights - RESTRICTED RIGHTS LEGEND. Use, duplication or disclosure by the Government is subject to restrictions as set forth in subparagraph (c)(1)(ii) of the Rights in Technical Data and Computer Software clause at DFARS 252.227-7013 or subparagraphs (c)(1) and (2) of the Commercial Computer Software - Restricted Rights clause at 48 CFR 52.227-19, as applicable. 17. Force Majeure - Waters shall have no liability for failure toperform, or delay in performance, in the delivery of any and all equipment manufactured or sold by Waters including instruments, supplies, components, systems, chemistry, accessories, replacement spare parts, or any and all services provided by Waters, caused by circumstances beyond its reasonable control including, but not limited to, acts of God, acts of nature, floods, fire, explosions, war or military mobilization, United States governmental action or inaction, request of governmental authority, delays of any kind in transportation or inability to obtain material or equipment, acts of other governments, strikes, or labor disturbances. 18. Diagnostic Products - Buyer acknowledges and agrees that only those products which are labeled and identified as in vitro diagnostic (“IVD”)

Waters Technologies Corporation dba Waters Corporation, 34 Maple St, Milford MA 01757 800 252 4752 This quotation is expressly conditioned upon, and subject to all terms and conditions set forth within Page: 6 / 7 Account : County of San Diego Quotation number : 22592188 Creation date : 04/13/2021 Expiration date : 05/14/2021

Sales Proposal Please reference this Quotation when Purchase Order is issued Waters General Sales Terms and Conditions devices are intended to be used for IVD purposes. Buyer acknowledges and agrees that any products that are not labeled and identified as IVDs are general laboratory products intended for research and other general scientific uses and are not for use in IVD procedures.

Waters Technologies Corporation dba Waters Corporation, 34 Maple St, Milford MA 01757 800 252 4752 This quotation is expressly conditioned upon, and subject to all terms and conditions set forth within Page: 7 / 7 REQUEST FOR BID (RFB) 10850 SHERIFF’S DEPARTMENT - CRIME LAB GRAND T LIQUID CHROMATOGRAPHY SYSTEM AND MAINTEANCE SERVICES EXHIBIT C - PAYMENT SCHEDULE (Submit completed Payment Schedule with your bid)

Make/Model Number: Acquity I-Class, Acquity RDa Company Name: Manufacturer: Waters Name: List Warranty Duration: 1 year Title: Lead Time: No more than 30 days Phone Number: Additional info (optional): Item 1 includes LCMS and nitrogen generator Email: Offeror qualifies as a Preferred Vendor* (if checked, complete information below.)

County maintains the right to verify the calculation of the Adjusted Price. In the event of a mathematical erro prevail, and a corrected Price Adjustment shall be used. TOTAL: $ 314,644.43

**Please refer to Waters Quotation #22592188 for detailed pricing information.

UNIT PRICE EXTENDED PRICE $ 212,544.73 $ 212,544.73 $ 4,907.95 $ 4,907.95 $ 5,079.75 $ 5,079.75 $ - $ - $ - $ - $ 23,028.00 $ 23,028.00 $ 23,028.00 $ 23,028.00 $ 23,028.00 $ 23,028.00 $ 23,028.00 $ 23,028.00 TOTAL $ 314,644.43

Waters Jinxi Li Mass Spectrometry Specialist 949-241-6227 [email protected]

referred Vendor as described in Section 405 of the a Preferred Vendor, Offeror must be a Local must document eligibility by satisfying both 1. and

ed from the following address(es)

Zip

d Business or Small Business.

Certificate # Certificate # Certificate # Certificate #

Certificate # Certificate #

ADJUSTED PRICE subtract Box 2 from Box 1

$ -

or, the Grand Total from the pricing schedule shall