RESEARCH HIGHLIGHTS

DOI: VESICULAR TRAFFICKING endocytic traffic. Furthermore, these 10.1038/nrn2166 structures became more numerous and elaborate with increasing Traffic jam at the age and density of the cortical cultures. Extracellular tracers during stimulation showed that although the synaptic vesicle formation, the formation of vesicles was not efficient authors were surprised to find that and recovery after stimulation took -1-knockout mice had an longer, vesicle formation did occur in apparently normal prenatal develop- dynamin-1-knockout mice. ment. However, these mice die within The researchers measured the net two weeks after birth which indicates rate of endo/ with the help that, postnatally, dynamin 1 is indis- of synapto-pHluorin, a chimaera pensable. of a synaptic vesicle and a The authors investigated synaptic fluorophore that is quenched at low transmission in primary cortical cul- pH, which is relevant as the lumen tures derived from knockout mice and of synaptic vesicles is acidified. showed that in electrophysiological Stimulation of recordings, although synaptic events release at increasingly high frequen- occurred, the peak amplitude of single cies will reach a threshold at which evoked miniature inhibitory postsy- can no longer keep naptic currents (mIPSCs) was greatly up with exocytosis, and therefore reduced in the absence of dynamin 1. the net rate of endo/exocytosis will Furthermore, sustained stimulation decline. Comparing cortical cultures led to a faster depression of mIPSCs, derived from wild-type and knockout with slower recovery. These findings mice, the authors observed that the suggest that synaptic transmission was threshold of this decline was reached less effective in the knockout mice, but at a lower stimulation frequency in unexpectedly was not abolished. the knockout cultures, consistent Investigating tissue sections and with impaired vesicle recovery. This cultured by electron micros- impairment of endocytosis could Electron micrograph of a involves a tightly copy, the authors noticed that synaptic be rescued by transfection with synapse from a cultured cortical of a controlled balancing act between vesicles were often heterogeneous in dynamin 1 and also by overexpres- dynamin-1-knockout mouse. synaptic vesicle exocytosis and endo- size and on average slightly larger in sion of dynamin 3, but only partially Note the presence of abnormally large vesicles cytosis. One of the key players in knockout samples, yet the number of by overexpression of dynamin 2. sparsely distributed among endocytosis is the GTPase dynamin, vesicles was only moderately reduced These results suggest overlapping, synaptic vesicles, and numerous coated which assembles at the neck of at most . This indicates that but also different, functions of the profiles with tubular necks. clathrin-coated pits and regulates dynamin 1 is not required for the three isoforms. Tomographic analysis of similar synapses indicated the fission of endocytic vesicles. In a biogenesis of synaptic vesicles. Ferguson et al. showed that that the most of these recent Science paper, Ferguson et al. Imaging studies using extracellular dynamin 1 has a key role during profiles are connected to the plasma membrane by showed that dynamin 1 is not tracers demonstrated the presence of endocytosis at synaptic sites under branched tubular networks. required for embryonal development intracellular, unusual, interconnected conditions of high synaptic activity. Image courtesy of S. Ferguson, S. Paradise and P. and is essential for endocytosis and and branched clathrin-coated vesicu- Further studies are required to shed De Camilli, Department of synaptic transmission only during lar structures that were continuous light on the mechanisms that these Biology, Howard high synaptic activity. with the plasma membrane of cul- mice use to compensate for the loss Hughes Medical Institute, Yale University School of There are three mammalian tured dynamin-1-knockout neurons. of dynamin 1 during development Medicine, New Haven, isoforms, dynamin 1–3, of which This is in stark contrast to the very and low-level synaptic stimulation. Connecticut, USA. dynamin 1 is by far the most few clathrin-coated profiles observed Claudia Wiedemann abundant in the . in wild-type neurons, generally single Clustering of synaptic vesicles at pits or single vesicles. This suggests ORIGINAL RESEARCH PAPER synaptic sites is a defining event in that the final stages of endocytosis Ferguson S. M. et al. A selective activity- dependent requirement for dynamin 1 in synaptic synapse formation. So, as dynamin 1 were impaired in dynamin-1-knock- vesicle endocytosis. Science 316, 570–574 (2007) was believed to be essential for out mice, resulting in a backup of

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