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CYP1A1 CYP1A2 CYP1B1 CYP3A4 Qiagen

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CYP1A1 CYP1A2 CYP1B1 CYP3A4 Qiagen Supplemental Table 1 Primer Size PCR PCR Genes Taq used Exons Primers Sequence primer 5'-3' [mM] product program* 2 2F1 cctgacactctagatattggc 0,3 571 TD 60°C 2R2 tccaggtagcaggaggttga 0,3 2F2 agtaatggtcagagcatgtcc 0,3 588 TD 60°C 2R2 aagccccatgcagttcctc 0,3 3-4 3 F aaggaccagacctggatgg 0,3 461 TD 56°C Qiagen 4R acagggacaagatggatgc 0,3 CYP1A1 Hot Star 5-6 5 F agtggctcccttcaaagggg 0,3 496 TD 60°C 6R tataagagcttaagagggtgg 0,3 7 7 F tggagctccactcacttgac 0,6 467 TD 56°C 7R tcagtgtctatgagtttcaggc 0,6 3'UTR P3'F actcaccctgaaccccattc 0,3 405 TD 60°C P3'R taggagtcttgtctcatgcct 0,3 5'UTR P5'F agcgttcatgttgggaatcttg 0,3 336 TD 56°C P5'R tcaaacccttgagcacccag 0,3 2 2F1 tatccagctgggagccaagc 0,3 512 TD 60°C 2R1 tctgtgctgaaggtcaagctc 0,3 2F3 acgtcctgcagatccgcattg 0,3 736 TD 64°C 2R2 aggtgtccagagccttccta 0,3 Qiagen 3 3 F agcaacgttcagcctttgacc 0,3 319 TD 64°C CYP1A2 Hot Star 3R tcccaagccagatctcagtatg 0,3 4-5 4 F tgatggtttccttgtttctgtc 0,6 601 TD 64°C 5R aggaagagaaaacatggcaagc 0,6 6 6 F tggaggtaggagcaacacat 0,6 233 TD 64°C 6R agttcctagcagggacaaac 0,6 7 7 F tgttctcaacagaagtctccc 0,3 467 TD 60°C 7R agaaaggaagagaaacaagggc 0,3 1 1F tgaaagcctgctggtagag 0,6 697 TD 60°C Qiagen 1R aggcgcgtaacggttcctg 0,6 Hot Star 2 2F1 acctctccacccaacggca 0,6 591 TD 60°C + Q 2R1 ggctggcgcgtgaagaagtt 0,6 CYP1B1 solution 2F2 acactcggagcactggaagg 0,6 714 TD 60°C 2R2 actcagcatattctgtctctac 0,6 Qiagen 3 3F1 agtcactgagctagatagcc 0,3 806 TD 56°C Hot Star 3R2 cagcacaaaagaggaactgg 0,3 CYP3A4 Qiagen 1 1F tgtgtgtgattctttgccaac 0,3 429 TD 60°C Hot Star 1R acgcccggcctgaacatct 0,3 2 2F tcttgccttaatgttacctcg 0,6 319 TD 60°C 2R tgtaaaacttcagaccttccc 0,6 3 3F acctcctctgttttctctt 0,6 317 TD 56°C 3R tcatcccaagaggctttgg 0,6 4 4F tccagctgtagaataaggctg 0,3 269 TD 64°C 4R tggacgtggaaccttcctgg 0,3 5/6 5F agcatctagcatagggccca 0,3 705 TD 60°C 6R actggaataacccaacagca 0,3 7 7F atgtgggtttcctgttgcat 0,3 376 TD 60°C 7R tgatggtcacacatatcttc 0,3 8 8F tggcttccagttgagaacc 0,3 393 TD 60°C 8R tgctctaaacatgagcagtc 0,3 9 9F aggaccacgcttgtgatttac 0,3 307 TD 60°C 9R tgcctctagaaagtgcctccaa 0,3 10 10F agggcttcacttagatttctc 0,3 383 TD 60°C 10R agagccttcctacatagagtc 0,3 11 11F tcattagtactgcatggactg 0,3 382 TD 60°C 11R tcctgtagattaagagaggc 0,3 12 12F catagcaggatttcaatgacc 0,3 447 TD 60°C 12R cagatgggcctaattgattct 0,3 13 13F agtgtctcactcactttgatgc 0,3 489 TD 64°C 13R aggaggagttaatggtgctaac 0,3 1 1F tccttctccagcacataaatc 0,3 341 TD 56°C 1R tccaacacttcagctacttc 0,3 2 2F tgaccctctgtgattgagtgc 0,3 653 TD 64°C 2R gtgaaacctcagaactccct 0,3 3 3F agacatctctgaatagcttcc 0,3 363 TD 56°C 3R ctactcccaagtaattatcc 0,3 4 4F tgtaccacccagcttaacg 0,3 573 TD 60°C 4R agattgcaagatgttaccac 0,3 5/6 5F tgtgcaaccatgaagatcacc 0,3 618 TD 64°C 6R gagtaacccaacagtgcgac 0,3 7 7F tagtggaaggacggtaagag 0,3 403 TD 56°C Qiagen 7R gacagctaaagtgtgtgaggg 0,3 CYP3A5 Hot Star 8 8F tgggttccagttgagaatca 0,3 390 TD 56°C 8R ctaccaaatggcttctcttgt 0,3 9 9F tcttcaagaagccataggg 0,6 383 TD 56°C 9R tggaacatggctatacctgt 0,6 10 10F tctaagctgtgatgttgtacg 0,6 350 TD 56°C 10R cctacattatgtcagtgaag 0,6 11 11F tgagttattctctggagcttc 0,3 433 TD 56°C 11R tgattgtacaaccacacgat 0,3 12 12F acccctaacatgtaactctg 0,3 362 TD 60°C 12R tcaaagatggatccaagtagg 0,3 13 13F actatccagtatttacccagc 0,3 479 TD 56°C 13R tggtcacctcctttatactac 0,3 COMT Qiagen 1 1 F gctacttgtggctagaagca 0,6 494 TD 56°C Hot Star + Q solution 1R tcacttcacagcggtccgaat 0,6 Qiagen 2 2 F tatctggaccttggtcatcgc 0,3 461 TD 56°C Hot Star 2R3 tggacgctccattttagaaag 0,3 3 3 F gatggtggcactccaagcaaa 0,3 455 TD 60°C 3R acagcttctcctgtaagggctt 0,3 4 4 F accagggaggtgaaatacc 0,3 497 TD 56°C 4R ctggtgatagtgggttttca 0,3 5 5 F tgacaggcgctgttgtatagg 0,3 281 TD 60°C 5R agaggctgaggctgactgaat 0,3 6 6 F tcctttcctgcaggagtcacg 0,3 484 TD 60°C 6R aggtgtgctttgcatttaggac 0,3 1 1F tcttgacttccaccagttgc 0,3 465 TD 56°C 1R aggtccctaatctcttccc 0,3 2/3 2F aggagatggaatgcaataacc 0,3 651 TD 64°C 3R tgcagtaaagtgggtaactg 0,3 Qiagen 4 4F actgtcaagttggctgacc 0,3 712 TD 56°C NQO1 Hot Star 4R aggcttaggatctaattgtgc 0,3 5 5F acagcaaataggacagacttg 0,3 571 TD 60°C 5R aggctcaatgaatgaattccc 0,3 6 6F aggcctccttatcagagtgtc 0,3 487 TD 64°C 6R agtcagggaagccttggaaag 0,3 1 1F1 atccacatgctcagactgttg 0,3 457 TD 60°C 1R1 aaggtctgaccatctcttaatc 0,3 1F2 atcccaacaactcatccgc 0,6 594 TD 56°C 1R2 aagctctgccttcaaagacac 0,6 2 2F acctcacaattctcctactac 0,6 467 TD 60°C 2R tcagtgtaagtcaaacactctg 0,6 Qiagen 3 3F tcttttggtagtgcccgctg 0,3 420 TD 60°C UGT2B7 Hot Star 3R tgtcagcatatttacatagggg 0,3 4 4F tcccttgatctcattcctactc 0,6 334 TD 56°C 4R tctttctcccttaagactgga 0,6 5 5F acactgtcactttcagagcc 0,3 373 TD 56°C 5R actcacactcactattgacaag 0,3 6 6F tccttctttaactcggtgtc 0,6 412 TD 56°C 6R actgaagtagtctcacctatc 0,6 *see PCR conditions at www.cephb.fr/tcf1 Supplemental Table 2 : CYP1B1 allelic frequencies in the groups of patients 55 women with a 98 women with a non Alleles mutated HNF1α mutated benign tumor adenoma * CYP1B1*1 9% 10% CYP1B1*2 25% 31% CYP1B1*3 40% 38% CYP1B1*4 24% 20% CYP1B1*6 1% 1% CYP1B1*7 1% 0% Chi-squared 3.224 P value 0.665 • unvailable data in three cases. Supplemental Figure 1 : : Effect of Y81N on CYP1B1 catalytic activity. Effect of Y81N polymorphism on CYP1B1 catalytic activity. The EROD activity is expressed as picomoles of resorufin per milligram of protein per minute and normalized to the total ß-galactosidase activity measuring the transfection efficiency. Values are means +/- S.D. (n = 4 replicates). EROD activity of the CYP1B1*3 allele was used as reference and fixed to 1. Values of other alleles were expressed according to it. Corresponding immunoblot analysis using an anti-CYP1B1 primary antibody are indicated by an arrow. .
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