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MMP-9 (92Kda Collagenase IV) Ab-9, Rabbit Polyclonal Antibody

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MMP-9 (92Kda Collagenase IV) Ab-9, Rabbit Polyclonal Antibody DATA SHEET Rev 091009K MMP-9 (92kDa Collagenase IV) Ab-9 Rabbit Polyclonal Antibody Cat. #RB-1539-P0, -P1, or -P (0.1ml, 0.5ml, or 1.0ml at 1.0mg/ml) (Purified Ab with BSA and Azide) Cat. #RB-1539-P1ABX or -PABX (0.5ml or 1ml at 1.0mg/ml) (Purified Ab without BSA and Azide) Description: MMPs are a group of enzymes involved in matrix degradation. They share some Limitations and Warranty: important characteristics, such as a common mode of Our products are intended FOR RESEARCH USE ONLY activation, a conserved amino acid sequence in the and are not approved for clinical diagnosis, drug use or therapeutic procedures. No products are to be construed as a putative metal binding-active site region, and inhibition recommendation for use in violation of any patents. We make by specific proteinase inhibitors known as tissue no representations, warranties or assurances as to the inhibitors of metalloproteinases (TIMPs). Reportedly, accuracy or completeness of information provided on our MMPs play a crucial role in tumor cell invasion and data sheets and website. Our warranty is limited to the actual metastasis. price paid for the product. NeoMarkers is not liable for any property damage, personal injury, time or effort or economic Mol. Wt. of Antigen: 92kDa (pro form) and loss caused by our products. ~86kDa (active form) Material Safety Data: Epitope: Middle region of MMP-9 This product is not licensed or approved for administration to humans or to animals other than the experimental animals. Species Reactivity: Human and Guinea pig. Does Standard Laboratory Practices should be followed when not react with mouse, rat and cow. Others-not known. handling this material. The chemical, physical, and toxicological properties of this material have not been Immunogen: A synthetic peptide from the middle thoroughly investigated. Appropriate measures should be region of human MMP-9. taken to avoid skin and eye contact, inhalation, and ingestion. The material contains 0.09% sodium azide as a preservative. Applications and Suggested Dilutions: Although the quantity of azide is very small, appropriate care • Western Blotting (Ab-5 is better) should be taken when handling this material as indicated (Ab 2-4µg/ml for 2 hrs at RT) above. The National Institute of Occupational Safety and The optimal dilution for a specific application should Health has issued a bulletin citing the potential explosion hazard due to the reaction of sodium azide with copper, lead, be determined by the investigator. brass, or solder in the plumbing systems. Sodium azide forms hydrazoic acid in acidic conditions and should be discarded Positive Control: Conditioned, serum-free medium in a large volume of running water to avoid deposits forming from (TPA-treated) human fibrosarcoma HT-1080 in metal drainage pipes. cells. For Research Use Only Cellular Localization: Cytoplasmic Supplied As: 1mg/ml of antibody purified from rabbit serum by Protein A chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at 1mg/ml Storage and Stability: Ab with sodium azide is stable for 24 months when stored at 2-8°C. Ab WITHOUT sodium azide is stable for 36 months when stored at below 0°C. Suggested References: 1. Paquet P, et al. Eur J Clin Invest 1998;28(7):528- 532. 2. Jackson CJ, et al. Int J Biochem Cell Biol 1997;29(10):1167-1177. Thermo Fisher Scientific Thermo Fisher Scientific Anatomical Pathology Anatomical Pathology 46360 Fremont Blvd. Manufactured by: 93-96 Chadwick Road, Astmoor Fremont, CA 94538, USA NeoMarkers Runcorn, Cheshire WA7 1PR, UK Tel: 1-510-771-1560 For Tel: 44-1928-562600 Fax: 1-510-771-1570 Lab Vision Corporation Fax: 44-1928-562627 http://www.thermo.com/labvision [email protected] DATA SHEET Rev 091009K MMP-9 (92kDa Collagenase IV) Ab-9 Rabbit Polyclonal Antibody Cat. #RB-1539-P0, -P1, or -P (0.1ml, 0.5ml, or 1.0ml at 1.0mg/ml) (Purified Ab with BSA and Azide) Cat. #RB-1539-P1ABX or -PABX (0.5ml or 1ml at 1.0mg/ml) (Purified Ab without BSA and Azide) Additional Suggested References: the presence of malignant astrocytomas, brain 1. DuPont BR, Linn R, Knight CB, Roodman GD, metastases, and carcinomatous meningitis. Cancer Sakaguchi AY, Lalley PA, Fournier RE, Leach RJ: 1998;82(5):923-930. Assignment of matrix metalloproteinase 9 (Mmp9) to mouse chromosome 2 bands H1-H2. Cytogenet Cell Genet 1996;74(1-2):118-119. 2. Mentzel K, Brauer R: Matrix metalloproteinases, IL-6, and nitric oxide in rat antigen-induced arthritis. Clin Exp Rheumatol 1998 May- Jun;16(3):269-276. 3. Sakalihasan N, Delvenne P, Nusgens BV, Limet R, Lapiere CM: Activatedforms of MMP2 and MMP9 in abdominal aortic aneurysms. J Vasc Surg 1996;24(1):127-133. 4. Yoshizaki T, Sato H, Furukawa M, Pagano JS: The expression of matrix metalloproteinase 9 is enhanced by Epstein-Barr virus latent membrane protein 1. Proc Natl Acad Sci U S A 1998;95(7):3621-3626. 5. Callen DF, Lane SA, Kozman H, Kremmidiotis G, Whitmore SA, Lowenstein M, Doggett NA, Kenmochi N, Page DC, Maglott DR, et al: Integration of transcript and genetic maps of chromosome 16 at near-1-Mb resolution: demonstration of a "hot spot" for recombination at 16p12. Genomics 1995;29(2):503-511. 6. Bertaux B, Hornebeck W: Tissue inhibitors of matrix metalloproteinases TIMP 1-2. Structures and functions. C R Seances Soc Biol Fil 1993;187(2):192-200. 7. Hyuga S, Nishikawa Y, Sakata K, Tanaka H, Yamagata S, Sugita K, Saga S, Matsuyama M, Shimizu S: Autocrine factor enhancing the secretion of M(r) 95,000 gelatinase (matrix metalloproteinase 9) in serum-free medium conditioned with murine metastatic colon carcinoma cells. Cancer Res 1994;54(13):3611- 3616. 8. Giraudon P, Thomasset N, Bernard A, Verrier B, Belin MF: Induction of MMP9 (92 kDa gelatinase) activity and expression of tissue inhibitor of metalloproteinase-2 mRNA (TIMP-2) in primitive neuroectodermal cells infected with retrovirus HTLV-I. Eur J Neurosci 1995;7(5):841-848. 9. Friedberg MH, Glantz MJ, Klempner MS, Cole BF, Perides G: Specific matrix metalloproteinase profiles in the cerebrospinal fluid correlated with Thermo Fisher Scientific Thermo Fisher Scientific Anatomical Pathology Anatomical Pathology 46360 Fremont Blvd. Manufactured by: 93-96 Chadwick Road, Astmoor Fremont, CA 94538, USA NeoMarkers Runcorn, Cheshire WA7 1PR, UK Tel: 1-510-771-1560 For Tel: 44-1928-562600 Fax: 1-510-771-1570 Lab Vision Corporation Fax: 44-1928-562627 http://www.thermo.com/labvision [email protected] .
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