DOCSLIB.ORG

Increasing Renewable Oil Content and Utility

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Increasing Renewable Oil Content and Utility University of Kentucky UKnowledge Theses and Dissertations--Plant and Soil Sciences Plant and Soil Sciences 2017 INCREASING RENEWABLE OIL CONTENT AND UTILITY William Richard Serson University of Kentucky, [email protected] Digital Object Identifier: https://doi.org/10.13023/ETD.2017.243 Right click to open a feedback form in a new tab to let us know how this document benefits ou.y Recommended Citation Serson, William Richard, "INCREASING RENEWABLE OIL CONTENT AND UTILITY" (2017). Theses and Dissertations--Plant and Soil Sciences. 89. https://uknowledge.uky.edu/pss_etds/89 This Doctoral Dissertation is brought to you for free and open access by the Plant and Soil Sciences at UKnowledge. It has been accepted for inclusion in Theses and Dissertations--Plant and Soil Sciences by an authorized administrator of UKnowledge. For more information, please contact [email protected]. STUDENT AGREEMENT: I represent that my thesis or dissertation and abstract are my original work. Proper attribution has been given to all outside sources. I understand that I am solely responsible for obtaining any needed copyright permissions. I have obtained needed written permission statement(s) from the owner(s) of each third-party copyrighted matter to be included in my work, allowing electronic distribution (if such use is not permitted by the fair use doctrine) which will be submitted to UKnowledge as Additional File. I hereby grant to The University of Kentucky and its agents the irrevocable, non-exclusive, and royalty-free license to archive and make accessible my work in whole or in part in all forms of media, now or hereafter known. I agree that the document mentioned above may be made available immediately for worldwide access unless an embargo applies. I retain all other ownership rights to the copyright of my work. I also retain the right to use in future works (such as articles or books) all or part of my work. I understand that I am free to register the copyright to my work. REVIEW, APPROVAL AND ACCEPTANCE The document mentioned above has been reviewed and accepted by the student’s advisor, on behalf of the advisory committee, and by the Director of Graduate Studies (DGS), on behalf of the program; we verify that this is the final, approved version of the student’s thesis including all changes required by the advisory committee. The undersigned agree to abide by the statements above. William Richard Serson, Student Dr. David Hildebrand, Major Professor Dr. Arthur Hunt, Director of Graduate Studies INCREASING RENEWABLE OIL CONTENT AND UTILITY ________________________________ DISSERTATION ________________________________ A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the College of Agriculture, Food and Environment at the University of Kentucky By William Richard Serson Lexington, Kentucky Director: Dr. David Hildebrand, Professor of Plant and Soil Sciences Lexington, Kentucky 2017 Copyright © William Richard Serson 2017 ABSTRACT OF DISSERTATION INCREASING RENEWABLE OIL CONTENT AND UTILITY Since the dawn of agriculture man has been genetically modifying crop plants to increase yield, quality and utility. In addition to selective breeding and hybridization we can utilize mutant populations and biotechnology to have greater control over crop plant modification than ever before. Increasing the production of plant oils such as soybean oil as a renewable resource for food and fuel is valuable. Successful breeding for higher oil levels in soybean, however, usually results in reduced protein, a second valuable seed component. We show that by manipulating a highly active acyl-CoA: diacylglycerol acyltransferase (DGAT) the hydrocarbon flux to oil in oilseeds can be increased without reducing the protein component. Compared to other plant DGATs, a DGAT from Vernonia galamensis (VgDGAT1A) produces much higher oil synthesis and accumulation activity in yeast, insect cells and soybean. Soybean lines expressing VgDGAT1A show a 4% increase in oil content without reductions in seed protein contents or yield per unit land area. Furthermore, we have screened a soybean fast neutrino population derived from M92-220 variety and found three high oil mutants that do not have reduced levels of protein. From the F2 plant populations we quantitatively pooled the high oil and low oil plants and performed comparative genomics hybridization (CGH). From the data it appears that two families have a 0.3 kb aberration in chromosome 14. We are performing further analysis to study this aberration and develop markers for molecular breeding. Mutagenic techniques are also useful for developing other traits such as early flowering varieties and adapting new high oil crops to a new region. Chia (Salvia hispanica) is an ancient crop that has experienced an agricultural resurgence in recent decades due to the high omega 3 fatty acid (ω-3) content of the seeds and good production potential. The area of cultivation has been expanded to Kentucky using mutagenized populations and the composition traits are similar to that of the original regions of cultivation in Central and South America. KEYWORDS: diacylglycerol acyltransferase, triacylglycerides, mutagenesis, comparative genomics hybridization, chia, soybean ____William R Serson June 28, 2017 INCREASING RENEWABLE OIL CONTENT AND UTILITY By William R. Serson David Hildebrand________ Director of Dissertation Arthur Hunt ___________ Director of Graduate Studies June 28, 2017___________ TABLE OF CONTENTS LIST OF TABLES ..................................................................................................................... v LIST OF FIGURES .................................................................................................................. vi Chapter 1 INTRODUCTION ..................................................................................................... 1 Soybean Oil Content and Composition .................................................................................. 1 Chia: Salvia hispanica .......................................................................................................... 10 Chapter 2 VgDGAT1A INCREASES RENEWABLE OIL CONTENT AND OLEIC ACID CONTENT IN SOYBEANS (Glycine max) ............................................................................ 20 Introduction .......................................................................................................................... 20 Materials and Methods ......................................................................................................... 22 cDNA cloning .................................................................................................................. 22 Expression in insect cells ................................................................................................. 23 Yeast microsome assays .................................................................................................. 23 Lipid analysis ................................................................................................................... 24 Seed-specific expression vector construction and soybean somatic embryo transformation .................................................................................................................. 26 Segregating Line Analysis ............................................................................................... 28 Results .................................................................................................................................. 28 In vitro assays of VgDGAT1A ........................................................................................ 28 Oil and protein content of transgenic soybeans expressing VgDGAT1A ....................... 29 Segregating Line Analysis ............................................................................................... 30 Fatty acid composition in high oil lines ........................................................................... 30 Discussion ............................................................................................................................ 31 Chapter 3 A LARGE COPY NUMBER VARATION ON CHROMOSOME 14 APPEARS TO INCREASE OIL CONTENT IN SOYBEANS ................................................................. 41 Introduction .......................................................................................................................... 41 Materials and Methods ......................................................................................................... 43 Genetic Material ............................................................................................................... 43 Backcrossing .................................................................................................................... 43 Tissue Sampling, Seed Composition and DNA Extraction ............................................. 43 NMR Methods ................................................................................................................. 44 CGH Analysis .................................................................................................................. 44 Results .................................................................................................................................. 44 Oil and Protein Content of Backcrosses .......................................................................... 44 CGH Analysis .................................................................................................................
Load more

Recommended publications
  • Oilseeds As Crop Biofactories for Industrial Raw Materials
    AOF Forum, 2004 Grain & chemical industry drivers Oilseeds as crop ! Global competition increasing biofactories for ! Downward pressure on price and market share industrial raw ! Need to diversify away from commodities ! Need to capture maximum value materials Allan Green ! Desirable to replace petroleum with renewable CSIRO Plant Industry sources of industrial raw materials ! Need for increased biodegradability of C O C O C O industrial products C C C C C C O C O C O C Opportunities for industrial use Seed oils are triglycerides ! Current Australian non-food use of vegetable oils ! Seed oils are comprised almost entirely of is about 12,000 tonnes (~3% of total oil usage) triglycerides composed of fatty acids ! Long-term opportunities exist for:- ! Oils are deposited in the seed in oilbodies - direct use in lubricants and inks - bio-diesel fuels (e.g. rapeseed ME) - specialty oleochemicals - pure fatty acids (e.g. oleic acid) - fatty acid derivatives (e.g. erucamide) - alkyl units for polymers (e.g. nylon) - biodegradable plastics - pharmaceutical proteins Fatty acids are like petrochemicals Oil composition can be changed ! Fatty acids are simply hydrocarbon chains ! Seed oils are needed only for energy of various lengths with a carboxyl group storage and release during germination (~COOH) at one end (i.e. no structural role) ! Fatty acids can have bond types or functional groups that allow them to be ! Fatty acid composition can therefore be cleaved or derivatised by chemical dramatically modified, provided that new processing fatty
    [Show full text]
  • Role of Epoxide Hydrolases in Lipid Metabolism
    Biochimie 95 (2013) 91e95 Contents lists available at SciVerse ScienceDirect Biochimie journal homepage: www.elsevier.com/locate/biochi Mini-review Role of epoxide hydrolases in lipid metabolism Christophe Morisseau* Department of Entomology and U.C.D. Comprehensive Cancer Center, One Shields Avenue, University of California, Davis, CA 95616, USA article info abstract Article history: Epoxide hydrolases (EH), enzymes present in all living organisms, transform epoxide-containing lipids to Received 29 March 2012 1,2-diols by the addition of a molecule of water. Many of these oxygenated lipid substrates have potent Accepted 8 June 2012 biological activities: host defense, control of development, regulation of blood pressure, inflammation, Available online 18 June 2012 and pain. In general, the bioactivity of these natural epoxides is significantly reduced upon metabolism to diols. Thus, through the regulation of the titer of lipid epoxides, EHs have important and diverse bio- Keywords: logical roles with profound effects on the physiological state of the host organism. This review will Epoxide hydrolase discuss the biological activity of key lipid epoxides in mammals. In addition, the use of EH specific Epoxy-fatty acids Cholesterol epoxide inhibitors will be highlighted as possible therapeutic disease interventions. Ó Juvenile hormone 2012 Elsevier Masson SAS. All rights reserved. 1. Introduction hydrolyzed by a water molecule [8]. Based on this mechanism, transition-state inhibitors of EHs have been designed (Fig. 1B). Epoxides are three atom cyclic ethers formed by the oxidation of These ureas and amides are tight-binding competitive inhibitors olefins. Because of their highly polarized oxygen-carbon bonds and with low nanomolar dissociation constants (KI) [9] [10].
    [Show full text]
  • Download Product Insert (PDF)
    PRODUCT INFORMATION (±)12(13)-EpOME Item No. 52450 Formal Name: (±)12(13)epoxy-9Z-octadecenoic acid Synonyms: (±)2,13-EODE, Isoleukotoxin, COOH (±)-Vernolic Acid MF: C18H32O3 FW: 296.5 Chemical Purity: ≥98% O Supplied as: A solution in methyl acetate NOTE: Relative stereochemistry shown in chemical structure Storage: -20°C Stability: ≥1 year Information represents the product specifications. Batch specific analytical results are provided on each certificate of analysis. Laboratory Procedures (±)12(13)-EpOME is supplied as a solution in methyl acetate. To change the solvent, simply evaporate the methyl acetate under a gentle stream of nitrogen and immediately add the solvent of choice. Solvents such as ethanol, DMSO, and dimethyl formamide purged with an inert gas can be used. The solubility of (±)12(13)-EpOME in these solvents is approximately 50 mg/ml. Further dilutions of the stock solution into aqueous buffers or isotonic saline should be made prior to performing biological experiments. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. If an organic solvent-free solution of (±)12(13)-EpOME is needed, it can be prepared by evaporating the methyl acetate and directly dissolving the neat oil in aqueous buffers. The solubility of (±)12(13)-EpOME in PBS (pH 7.2) is approximately 1 mg/ml. We do not recommend storing the aqueous solution for more than one day. Description (±)12(13)-EpOME is the 12,13-cis epoxide form of linoleic acid (Item Nos. 90150 | 90150.1 | 21909).1,2 It is formed primarily via linoleic acid metabolism by the cytochrome P450 (CYP) isoforms CYP2J2, CYP2C8, and CYP2C9, however, CYP1A1 can contribute to (±)12(13)-EpOME production when pharmacologically induced.2 (±)12(13)-EpOME (500 µM) induces mitochondrial dysfunction and cell death in renal proximal tubule epithelial cells.
    [Show full text]
  • Epomes Act As Immune Suppressors in a Lepidopteran Insect
    www.nature.com/scientificreports OPEN EpOMEs act as immune suppressors in a lepidopteran insect, Spodoptera exigua Mohammad Vatanparast1, Shabbir Ahmed1, Dong‑Hee Lee2, Sung Hee Hwang3, Bruce Hammock3 & Yonggyun Kim1* Epoxyoctadecamonoenoic acids (EpOMEs) are epoxide derivatives of linoleic acid (9,12‑octadecadienoic acid) and include 9,10‑EpOME and 12,13‑EpOME. They are synthesized by cytochrome P450 monooxygenases (CYPs) and degraded by soluble epoxide hydrolase (sEH). Although EpOMEs are well known to play crucial roles in mediating various physiological processes in mammals, their role is not well understood in insects. This study chemically identifed their presence in insect tissues: 941.8 pg/g of 9,10‑EpOME and 2,198.3 pg/g of 12,13‑EpOME in fat body of a lepidopteran insect, Spodoptera exigua. Injection of 9,10‑EpOME or 12,13‑EpOME into larvae suppressed the cellular immune responses induced by bacterial challenge. EpOME treatment also suppressed the expression of antimicrobial peptide (AMP) genes. Among 139 S. exigua CYPs, an ortholog (SE51385) to human EpOME synthase was predicted and its expression was highly inducible upon bacterial challenge. RNA interference (RNAi) of SE51385 prevented down‑regulation of immune responses at a late stage (> 24 h) following bacterial challenge. A soluble epoxide hydrolase (Se-sEH) of S. exigua was predicted and showed specifc expression in all development stages and in diferent larval tissues. Furthermore, its expression levels were highly enhanced by bacterial challenge in diferent tissues. RNAi reduction of Se‑sEH interfered with hemocyte‑spreading behavior, nodule formation, and AMP expression. To support the immune association of EpOMEs, urea‑based sEH inhibitors were screened to assess their inhibitory activities against cellular and humoral immune responses of S.
    [Show full text]
  • Download Product Insert (PDF)
    PRODUCT INFORMATION (±)12(13)-EpOME-d4 Item No. 10009996 Formal Name: (±)12(13)epoxy-9Z-octadecenoic-9,10,12,13-d4 acid Synonyms: (±)12,13-EODE-d4, Isoleukotoxin-d4, (±)-Vernolic Acid-d4 D D MF: C18H28D4O3 COOH FW: 300.5 Chemical Purity: ≥98% Deuterium DDO Incorporation: ≥99% deuterated forms (d1-d4); ≤1% d0 Supplied as: A solution in methyl acetate NOTE: Relative stereochemistry shown in chemical structure Storage: -20°C Stability: ≥1 year Information represents the product specifications. Batch specific analytical results are provided on each certificate of analysis. Laboratory Procedures (±)12(13)-EpOME-d4 contains four deuterium atoms at the 9, 10, 12, and 13 positions. It is intended for use as an internal standard for the quantification of (±)12(13)-EpOME (Item No. 52450) by GC- or LC-MS. The accuracy of the sample weight in this vial is between 5% over and 2% under the amount shown on the vial. If better precision is required, the deuterated standard should be quantitated against a more precisely weighed unlabeled standard by constructing a standard curve of peak intensity ratios (deuterated versus unlabeled). (±)12(13)-EpOME-d4 is supplied as a solution in methyl acetate. To change the solvent, simply evaporate the methyl acetate under a gentle stream of nitrogen and immediately add the solvent of choice. Solvents such as ethanol, DMSO, and dimethyl formamide purged with an inert gas can be used. The solubility of (±)12(13)-EpOME-d4 in is these solvents is approximately 50 mg/ml. Description (±)12(13)-EpOME-d4 is intended for use as an internal standard for the quantification of 12(13)- EpOME by GC- or LC-MS.
    [Show full text]
  • Survey of the Fatty Acid Composition of Peanut (Arachis Hypogaea) Germplasm and Characterization of Their Epoxy and Eicosenoic Acids Earl G
    Food Science and Human Nutrition Publications Food Science and Human Nutrition 10-1-1997 Survey of the fatty acid composition of peanut (arachis hypogaea) germplasm and characterization of their epoxy and eicosenoic acids Earl G. Hammond Iowa State University Daniel Duvick Iowa State University Tong Wang Iowa State University, [email protected] Hortense Dodo Alabama A&M University R. N. Pittman United States Department of Agriculture Follow this and additional works at: http://lib.dr.iastate.edu/fshn_ag_pubs Part of the Food Science Commons, and the Human and Clinical Nutrition Commons The ompc lete bibliographic information for this item can be found at http://lib.dr.iastate.edu/ fshn_ag_pubs/17. For information on how to cite this item, please visit http://lib.dr.iastate.edu/ howtocite.html. This Article is brought to you for free and open access by the Food Science and Human Nutrition at Iowa State University Digital Repository. It has been accepted for inclusion in Food Science and Human Nutrition Publications by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. Survey of the fatty acid composition of peanut (arachis hypogaea) germplasm and characterization of their epoxy and eicosenoic acids Abstract Peanut (Arachis hypogaea) plant introductions (732) were analyzed for fatty acid composition. Palmitate varied from 8.2 to 15.1%, stearate 1.1 to 7.2%, oleate 31.5 to 60.2%, linoleate 19.9 to 45.4%, arachidate 0.8 to 3.2%, eicosenoate 0.6 to 2.6%, behenate 1.8 to 5.4%, and lignocerate 0.5 to 2.5%.
    [Show full text]
  • Highaperformance Liquid Chromatography of the Triacylglycerols of Vernonia Galamensis and Crepis Alpina Seed Oils W.E
    7 00' "Ii 449 d v V .!.. HighaPerformance Liquid Chromatography of the Triacylglycerols of Vernonia galamensis and Crepis alpina Seed Oils W.E. Neff*, R.O. Adlof, H. Konishi1 and D. Weisleder Food Quality and Safety Research, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604 The triacylglycerols of Vernonia galamensis and Crepis tirely suitable for intact TAG analysis. For example, Christie alpina seed oils were characterized because these oils have (9) indicated that TAG with unsaturated FA may undergo high concentrations of vernolic (cis·12,13-epoxy-cis-9-octa­ possible thermal alteration due to the high temperature re­ decenoic) and crepenynic (cis-9-octadecen-12-ynoic) acids, quired for the GC analysis. Christie (9) reviewed methods respectively. The triacylglycerols were separated from for TAG analysis without thermal alteration by reversed­ other components of crude oils by solid-phase extraction, phase high-performance liquid chromatography (RP-HPLC) followed by resolution and quantitation of the individual and concluded that the best HPLC method for characteriz­ triacylglycerols by reversed-phase high-performance liquid ing unsaturated TAG is a mobile gradient of acetonitrile and chromatography with an acetonitrile/methylene chloride methylene chloride, and a detector based on the transport gradient and flame-ionization detection. Isolated triacyl­ flame-ionization principle (FID), which gives a quantitative glycerols were characterized by proton and carbon nuclear response (10). magnetic resonance and by capillary gas chromatography We report here a qualitative and quantitative TAG analy­ of their fatty acid methyl esters. The locations of the fat­ sis of Vernonia galamensis and Crepis alpina by RP-HPLC­ ty acids on the glycerol moieties in the oils were obtained FID, as well as a direct stereospecific analyses of the TAGs by lipolysis.
    [Show full text]
  • Aberystwyth University Vernonia Galamensis and Vernolic Acid Inhibit
    Aberystwyth University Vernonia galamensis and vernolic acid inhibit fatty acid biohydrogenation in vitro Ramos Morales, E.; McKain, N.; Gawad, R. M. A.; Hugo, A.; Wallace, R. J. Published in: Animal Feed Science and Technology DOI: 10.1016/j.anifeedsci.2016.10.002 Publication date: 2016 Citation for published version (APA): Ramos Morales, E., McKain, N., Gawad, R. M. A., Hugo, A., & Wallace, R. J. (2016). Vernonia galamensis and vernolic acid inhibit fatty acid biohydrogenation in vitro. Animal Feed Science and Technology, 222, 54-63. https://doi.org/10.1016/j.anifeedsci.2016.10.002 General rights Copyright and moral rights for the publications made accessible in the Aberystwyth Research Portal (the Institutional Repository) are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. • Users may download and print one copy of any publication from the Aberystwyth Research Portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the Aberystwyth Research Portal Take down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. tel: +44 1970 62 2400 email: [email protected] Download date: 25. Sep. 2021 Elsevier Editorial System(tm) for Animal Feed Science and Technology Manuscript Draft Manuscript Number: ANIFEE-16-7735R2 Title: Vernonia galamensis and vernolic acid inhibit fatty acid biohydrogenation in vitro Article Type: Research Paper Section/Category: Ruminants Keywords: biohydrogenation, conjugated linoleic acid, rumen, vernolic acid, Vernonia galamensis Corresponding Author: Dr.
    [Show full text]
  • Identification of Two Epoxide Hydrolases in Caenorhabditis
    Available online at www.sciencedirect.com ABB Archives of Biochemistry and Biophysics 472 (2008) 139–149 www.elsevier.com/locate/yabbi Identification of two epoxide hydrolases in Caenorhabditis elegans that metabolize mammalian lipid signaling molecules Todd R. Harris a, Pavel A. Aronov a, Paul D. Jones b, Hiromasa Tanaka a, Michael Arand c, Bruce D. Hammock a,* a Department of Entomology and Cancer Research Center, University of California, One Shields Avenue, Davis, CA 95616, USA b International Flavors and Fragrances, 1515 State Highway 36, Union Beach, NJ 07735, USA c Institute of Pharmacology and Toxicology, University of Zu¨rich, Winterthurerstrasse 190, CH-8057 Zu¨rich, Switzerland Received 11 September 2007, and in revised form 12 January 2008 Available online 31 January 2008 Abstract We have identified two genes in the genomic database for Caenorhabditis elegans that code for proteins with significant sequence similarity to the mammalian soluble epoxide hydrolase (sEH). The respective transcripts were cloned from a mixed stage cDNA library from C. elegans. The corresponding proteins obtained after recombinant expression in insect cells hydrolyzed standard epoxide hydrolase substrates, including epoxyeicosatrienoic acids (EETs) and leukotoxins (EpOMEs). The enzyme activity was inhibited by urea-based compounds originally designed to inhibit the mammalian sEH. In vivo inhibition of the enzymes using the most potent of these com- pounds resulted in elevated levels of the EpOMEs in the nematode. These results suggest that the hydrolases are involved in the metab- olism of possible lipid signaling molecules in C. elegans. Ó 2008 Elsevier Inc. All rights reserved. Keywords: Soluble epoxide hydrolase; Lipid signaling; Epoxyeicosatrienoic acid; Leukotoxin; 9,10-Epoxy-12-octadecenoate; 12,13-Epoxy-9-octadecenoate Soluble epoxide hydrolase (sEH)1, an enzyme first char- In mammals, the epoxide hydrolase activity of sEH has acterized in mammals, hydrolyzes an epoxide moiety been implicated in the metabolism of several epoxide-con- through the addition of water [1,2].
    [Show full text]
  • PDF (Otey Finalthesis.Pdf)
    STRUCTURAL AND FUNCTIONAL EXPLORATION OF AN ARTIFICIAL FAMILY OF CYTOCHROMES P450 Thesis by Christopher R. Otey In Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy CALIFORNIA INSTITUTE OF TECHNOLOGY Pasadena, California 2007 (Defended October 30th, 2007) ii © 2007 Christopher R. Otey All Rights Reserved iii ACKNOWLEDGEMENTS The best feature of CalTech is the diversity and intelligence of the people one has an opportunity to work and interact with. The broad perspectives and vast knowledge base allows one to learn and grow in different ways each and every day. I have developed a lot of great friendships that I hope will last long into the future and I want to thank everyone I have come in contact with for how they have helped me grow as a person. In the laboratory, everyone’s assistance has been invaluable but I especially want to thank a few individuals. I want to start with Joff Silberg, who provided a great deal of mentorship during the early stages of my research and was invaluable in providing guidance and inspiration. I am thankful I could always go to him with questions, to get ideas and to get back on track. Kaori Hiraga was a joy to work with and developed SISDC and was extremely helpful in constructing the chimeric P450 library, both times we made it! I must thank the ‘theoretical’ folks in the lab; Jeff Endelman, Allan Drummond, Jesse Bloom, Chris Voigt and Chris Snow. Their assistance has proven to me once and for all that they do exist. I must especially thank Jeff Endelman and Chris Voigt for their direct contributions in the form of logistic regression tools and the SCHEMA algorithm.
    [Show full text]
  • Ligand Access Channels in Cytochrome P450 Enzymes
    Ligand Access Channels in Cytochrome P450 Enzymes: A Review Francisco Esteves, Diana Campelo, Sophie Bozonnet, Jose Rueff, Michel Kranendonk, Philippe Urban, Thomas Lautier, Denis Pompon, Gilles Truan To cite this version: Francisco Esteves, Diana Campelo, Sophie Bozonnet, Jose Rueff, Michel Kranendonk, et al.. Lig- and Access Channels in Cytochrome P450 Enzymes: A Review. International Journal of Molecular Sciences, MDPI, 2018, 19 (6), pp.134 - 140. 10.3390/ijms19061617. hal-01903000 HAL Id: hal-01903000 https://hal.archives-ouvertes.fr/hal-01903000 Submitted on 26 May 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Distributed under a Creative Commons Attribution| 4.0 International License International Journal of Molecular Sciences Review Ligand Access Channels in Cytochrome P450 Enzymes: A Review Philippe Urban * ID , Thomas Lautier, Denis Pompon and Gilles Truan * Laboratoire d’Ingénierie des Systèmes Biologiques et des Procédés, Université de Toulouse, CNRS, INRA, INSA, 31000 Toulouse, France; [email protected] (T.L.); [email protected] (D.P.) * Correspondence: [email protected] (P.U.); [email protected] (G.T.) Received: 18 April 2018; Accepted: 28 May 2018; Published: 30 May 2018 Abstract: Quantitative structure-activity relationships may bring invaluable information on structural elements of both enzymes and substrates that, together, govern substrate specificity.
    [Show full text]
  • Aberystwyth University Vernonia Galamensis and Vernolic Acid
    Aberystwyth University Vernonia galamensis and vernolic acid inhibit fatty acid biohydrogenation in vitro Ramos Morales, E.; McKain, N.; Gawad, R. M. A.; Hugo, A.; Wallace, R. J. Published in: Animal Feed Science and Technology DOI: 10.1016/j.anifeedsci.2016.10.002 Publication date: 2016 Citation for published version (APA): Ramos Morales, E., McKain, N., Gawad, R. M. A., Hugo, A., & Wallace, R. J. (2016). Vernonia galamensis and vernolic acid inhibit fatty acid biohydrogenation in vitro. Animal Feed Science and Technology, 222, 54-63. https://doi.org/10.1016/j.anifeedsci.2016.10.002 General rights Copyright and moral rights for the publications made accessible in the Aberystwyth Research Portal (the Institutional Repository) are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. • Users may download and print one copy of any publication from the Aberystwyth Research Portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the Aberystwyth Research Portal Take down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. tel: +44 1970 62 2400 email: [email protected] Download date: 03. Oct. 2019 Elsevier Editorial System(tm) for Animal Feed Science and Technology Manuscript Draft Manuscript Number: ANIFEE-16-7735R2 Title: Vernonia galamensis and vernolic acid inhibit fatty acid biohydrogenation in vitro Article Type: Research Paper Section/Category: Ruminants Keywords: biohydrogenation, conjugated linoleic acid, rumen, vernolic acid, Vernonia galamensis Corresponding Author: Dr.
    [Show full text]