FI:DP/CPR/88/077 Field Document 9 December 1990

PEOPLE'S REPUBLIC OF CHINA

EQUIPMENT REQUIREMENTS

Report prepared for the project Fisheries development in Qinghai Province People's Republic of China

by C.J. Smith Consultant

FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS Rome, 1990 TABLE OF CONTENTS Page 1 INTRODUCTION AND TERMS OF REFERENCE 1 2 CURRENT STATUS OF EQUIPMENT 1 3 CURRENT STATUS OF PERSONNEL 1 Appendix I EQUIPMENT CURRENTLY AT THE INSTITUTE OF AQUATIC 3 PRODUCTS Appendix II: SPECIFICATIONS - LOTS 1-11 4 1. INTRODUCTION AND TERMS OF REFERENCE The project aims to assist development of fish production in Qinghai Province in two ways: by producing a management plan for the important naked carp fishery on Qinghai Lake and by demonstration of the technology of rainbow trout culture. This report identifies the equipment and monitoring requirements of the project by the assessment of: – the current equipment status of the laboratory of the Institute of Aquatic Products, Xining City, Qinghai Province, People's Republic of China – the equipment requirements of the project to enable a basic limnological investigtion to be undertaken in line with the objectives of the project. As part of the project operations, FAO assigned Mr C.J. Smith as consultant from 6 September to 25 October 1989. After consul-tations with project Chief Technical Adviser, Mr David Edwards and with FAO HQ staff in Rome the consultant's terms of reference were modified to take account of the above requirements. 2. CURRENT STATUS OF EQUIPMENT The laboratory at the Institute of Aquatic Products is in a dilapidated condition; however, there are number of old but functional pieces of equipment (listed in Appendix ]). Apart from a small portable pHmeter, all equipment appears fixed. 3. CURRENT STATUS OF PERSONNEL After discussion with the present staff of the Institute and observations on the status of the laboratory and the current work programme, it is evident that, although the level of training appears to be adequate, there is a considerable lack of practical experience both in the field and in the laboratory. It is assumed that this could be rectified by a relatively short training programme before the full project field work commences. Detailed discussions with the CTA and HQ staff in Rome have stressed the need to undertake limnological investigations only inasfar as they affect the fishery. It is not the purpose of this project to conduct a detailed academic survey of the environment and biota of Qinghai Lake. Consequently, numerous detailed precise analyses of a large variety of parameters were not necessary. It is essential, however, that in keeping the institution-building aspects of this project, a complete, operational laboratory is equipped with the necessary analytical and investigative equipment. To this end, the equipment requirements have been kept to a minimum and emphasis placed on any physical and chemical analyses being carried out, where possible, in the field, with the fixed laboratory facility in Xining City being used for those parameters not easily tested in the field (e.g., BOD, COD, protein, etc.) and for occasional confirmatory analyses of the performance of the field kits. The equipment required is listed in Appendix II, and is divided into a series of lots for easy categorization and procurement. Assessment has been made of some of the available models on the market, and where appropriate, recommendations made as to the best available option. This decision has been based on delivery, cost, technical specia1ications, previous "hands-on" experience of the consultant, and first-hand knowledge of the project site and capabilities of local staff. It is imperative that all the equipment noted is ordered and delivered without delay in order that the project is ready to commence in March 1990 with the personnel of the Institute trained in its use. It is understood that throughout the project's 4-year span, provision must be made for the replacement of consumable items and the purchase of technical literature as it is identified. The costings shown are indicate and precise costings must await pro-forma invoices from suppliers, and will be subject to currency exchange rate fluctuations. For the purposes of this report a conversion rate of £1 = US$ 1.60 is applied. Given that the limnological investigations should pertain to fisheries aspects only, the monitoring programme needs to be very flexible once the survey vessel starts work in spring 1990. The provision of a depth profiler to carry out determinations of pH, temperature, conductivity, dissolved oxygen and light intensity at depth, will considerably reduce the number of analyses needed to establish a baseline reference for the identification of water masses. Once this baseline is established, areas of the lake important in terms of the fishery can be examined in more detail. In order that remote upstream spawning sites can be visited and examined, and the fact that the difficult terrain in the area precludes visits by four-wheel vehicles overland, or by the workboats upstream, a recommendation is made f or the purchase of 2 off-road motorcycles. APPENDIX I Equipment currently at the Institute of Aquatic Products, Xining City, Qinghai Province, People's Republic of China NON-EXPENDABLES Electronic, double pan balance and weights 1 Manual, double pan balance, with weights 1 Electric, distilled water still, 10L/hour 1 20L/hour 1 Electric Oven, temperature adjustable (Note 1) 50–250°C 1 Electric muffle furnace (Note 2) range 0–950°C 1 Spectrophotometer, range 360nm–800nm, plus cuvettes.(Note 3). l Spectrophotometer, range 420nm–700nm, plus cuvettes.(Note 3). l pH meter, portable 1 pH and redox potential meter, fixed, 1 Centrifuge, cup capacity 250 ml 1 Dessicators, glass, non-vacuum 3 Water bath, electric, four rings, rectangular 1 Beam balance, maximum load lOOOg 1 EXPENDABLES (Note 4) Beakers, mainly 500ml capacity 25 Flasks, Erlenmeyer, 500,250 and 100 ml capacity 30 Flasks, volumetric, 500,250 and 100 ml capacity 30 Pipettes, bulb and straight type, all capacities 50 Burettes, ‘B’ grade accuracy, 50ml capacity 15 Watch glasses, various sizes 50 Bottles, dropping,100, 50 and 25ml capacity 10 Bottles, sample, 500ml capacity 5 Bottles, wash, operated by mouth, 1L capacity 2 Bottles, reagent, various sizes, plastic stoppers 20 Bottles, BOD, ground glass stoppers 2 Condensers, coil type, 2 Test tubes, with stoppers, 50ml capacity 30 Filter funnels, mainly small sizes 5 Measuring cylinders, 100, 250, and 500ml capacity 20 NOTES: 1. Thermostat broken. To be purchased locally. 2. Not connected, but assured is in working order. 3. Old model, but apparently in working order. 4. Many pieces of glassware contain chemicals or solutions that may render washing and re-use impractical. APPENDIX II LOT 1 LABORATORY CONSUMABLES LOT 2 SURVEY EQUIPMENT LOT 3 RESEARCH MICROSCOPE LOT 4 TRANSPORT LOT 5 LAKE CHEMISTRY PROFILER LOT 6 DATA COLLECTION APPARATUS LOT 7 SURVEY VESSEL REQUISITES LOT 8 FISHING NETS LOT 9 NAVIGATION AND COMMUNICATION LOT 10 LITERATURE LOT 11 CHEMICALS APPENDIX II LOT 1 SPECIFICATIONS UNIT No. TOTAL COST COST (USD) (USD) 1.1. Pressure tubing, red, rubber 5mm bore, 13mm outside diameter, 10m coil, 4mm thick wall. Catalogue No.TMG134 17.60 1 17.60 1.2 Red rubber tubing, general laboratory purpose grade , 5mm bore, 8mm outside diameter, 1.5mm wall, 10m coil. Catalogue No.TMG116 7.36 1 7.36 1.3 Sample bottles, square, wide mouth with push in leakproof seals and easy to grip caps. Caps must have a toggle for attachment of label. HDPE or similar material 2L capacity Catalogue No.BGE242 3.81 500 1905.00 1.4 As 1.3 but 1L capacity Catalogue No.BGE240 2.64 100 264.00 1.5 As 1.3 but 500ml capacity Catalogue No.BGE236 2.09 100 209.00 1.6 As 1.3 but 50ml capacity and LDPE material Catalogue No.BGE218 0.81 50 40.50 1.7 Round, wide mouth, HDPE, robust, aspirators. Fitted with minimum 12mm bore stopcock welded to the bottle. Minimum of two carrying handles. 50L capacity. Catalogue No.BGH070 94.08 2 188.16 1.8 Round, sloping shoulder, LDPE, distilled water wash bottles. Minimum capacity 250ml with screw cap leakproof fitting and swivel delivery tubes Catalogue No.WGF530 1.55 8 12.40 1.9 Thermometers, total immersion, 300mm long, glass, yellow back glass, mercury filled with reinforced bulb. -10°C to 1°C in 1°C gradation. Catalogue No. TNG040 2.40 10 24.00 1.10 Glass microscope slide, sandblasted edges, 1.0 to 1.2mm nomimal thickness, 76x26mm, plain. “Blue Star” or similar type. Catalogue No.MLP024 0.0258 500 12.90

1.11 Cover slips, glass, for item 1.11, 22mm square, thickness, 0.155mm – 0.185mm Catalogue No.MLP058 0.024 1000 24.00 1.12 Metal bottle store to meet Highly Inflammable Liquids Regulations. Capacity 8 × 2.5L Winchester type bottles, with partitioning trays and two carrying handles. Catalogue No.BMP176 175.20 3 525.60 1.13 Beakers, borosilicate glass, with spout and graduations, low form, Duran type, 50ml capacity Catalogue No.BKZOOO 2.18 50 109.00 1.14 As 1.13 but 100ml capacity Catalogue No.BKZ002 2.10 50 105.00 1.15 As 1.13 but 250ml capacity Catalogue No.BKZOO6 2.29 50 114.50 1.16 As 1.13 but 1000ml capacity Catalogue No.BKZO16 5.39 10 53.90 1.17 Kjeldahl apparatus comprising, electothermal 220/240AC heating mantle with six heating recesses to take 100 -300ml capacity Kjeldahl flask apparatus total wattage 1800v maximum. 6 complete Kjeldahl “Quickfit” assemblies comprising condenser, dropping funnel, Kjeldahl flask, multiple adapter, spring clips, delivery adapter and splash head. Catalogue No. KLL012 × 1 KLL000 × 6 1163.84 1 1163.84 1.18 Glass, Petri dishes, borosilicate, with lid and base, size 100mm × 20mm. Catalogue No. PLX012 4.53 50 226.50 1.19 Plastic, disposable, gloves Catalogue No.GJU028 0.02 200 4.00 1.20 Asbestos gloves, cotton lined, single palm, 460mm long per pair, medium size Catalogue No.GJU020 23.52 4 94.08 1.21 Rubber, heavy duty gloves, medium size. Catalogue No.GJUO66 2.64 4 10.56 1.22 Cotton drill, laboratory coats, with buttons, 3 patch pockets and centre back vent, medium size. Catalogue No.LJU038 21.26 10 212.60 1.23 Safety spectacles, single clear polycarbonate construction, light weight, able to be worn with prescription glasses. Grade 2 impact standard. Catalogue NO.EKP042 2.53 10 25.30 1.24 Plastic bin with lid, minimum capacity 50L in rigid polyethylene, Catalogue No.BWX036 173.54 10 1735.40 1.25 Plastic bags, clear polyethylene with fastening grip for sealing and resealing, minimum size 152x229mm. Catalogue No.BMQ184 0.05 1000 50.00 1.26 Pipette filler, single hand operation, three valves for pressure release, suction and delivery by gravity or pressure. Capable of use with pipettes from 10ml capacity upwards. Catalogue No.PWY145 7.45 10 74.50 1.27 Watchmakers, eye held, magnifying glass, plastic casing, × 6 magnification. No catalogue Number 10.00 10 100.00 1.28 Hand held magnifying glass, in chrome frame with black plastic handle, lens diameter 90mm, × 3 magnification. Catalogue No.MLQ102 4.70 10 47.00 1.29 Folding, pocket type, magnifying glass lens diameter 13mm, × 10 magnification. Catalogue No. MLQOO8 8.64 10 86.40 1.30 Anenometer, hand held, portable readings available directly in km/hour. Beaufort scale, metres/sec Accuracy 3km/hr. Catalogue No.ALM052 120.00 1 120.00 1.31 Sediment sieves, aluminium or brass, stacking type, mesh sizes 10,20,40,60,80, and 100. Complete with collection pan and lid. Catalogue No.SMH062 56.00 1 56. 00 1.32 Photographic trays, 1L capacity, with lid, white, polypropylene for dissection and examination. Catalogue No.TWR202 4.10 10 41.00 1.33 As 3.3 but 10L capacity. Catalogue No.TWR204 9.89 10 98.90 1.34 Binocular microscope, daylight lit, inclined movable binocular head (magnification x1.5). Supplied with x7 and x10 paired eyepieces, and x8 x40 and x90 objectives. Complete with carrying case. Catalogue No.MLQ042 392.40 1 392.40 1.35 Steroscopic microscope for dissection and field work, large field, daylight lit, 150mm diameter base with black/white reversible disc and metal clips. Magnification × 8.75. Supplied in woodencase. Catalogue No.MLQ046 96.00 2 192.00 1.36 Stereoscopic microscope for research and precision operations. Large field artificial light from 8v, 20w 220/240AC supply fitted to body of microscope. Working distance 64mm. Supplied with x6 x8, x12 and x14 eyepieces, and x0.6, x1, x2, x4 and x7 objectives Catalogue No. MLQ034 592.00 2 1184.00 1.37 Haemocytometer, “Neubauer” type counting chamber single chamber, with mouthpiece, tubing and case Catalogue No.HLF074 32.40 2 64.80 1.38 Dissection kit comprising scalpel handles, blades, holders, scissors, forceps, seekers, needles etc. in cloth carrying case with individual pocket inserts Catalogue No.DKG184 55.10 10 551.00 1.39 Tally counter, hand operated, 4 clear digits and reset key in metal surround Catalogue No.TMR290 24.00 10 240.00 1.40 Medium sized, vacuum pump,1.5m3/hour 185mm bar, motor rating 0.09kw, 220/240AC supply. Catalogue No.VNJ014 333.52 1 333.52 1.41 Small, oil free silent pump for creating vacuum. 18L/minute, 500mm Hg pressure,2kg/cm2. 220/240V AC Catalogue No.PNJ114 183.50 2 367.00 1.42 Membrane filter holders, borosilicate glass with stainless steel filter support and screen. 47mm membrane diameter with 300ml reservior capacity. Catalogue No.MLN176 155.71 6 934.27 1.43 Glass fibre papers, Grade GF/B, 1mm particle retention 47mm diameter. Catalogue No.FKR538 0.20 5000 1000.00 1.44 Imhoff Sedimentation system comprising rectangular plastic stand with 3 holes and locating depressions in base Catalogue No.SWXOO6 135.30 1 135.30 1.45 Imhoff cones of 1L capacity with stoppers. Catalogue No.SWXOO8 47.12 3 141.36 1.46 “Grant” water bath, temperature range 50°C above ambient to 150°C, complete with heaters, pump stirrer and temperature sensor. 38L total capacity 220/240V AC. Catalogue No.WTB012 672.00 1 672.00 1.47 Electric centrifuge to take up to 8xl5ml samples at up to 7000rpm producing 5400g with electronic braking. Electronic lid lock, 220/240v AC. Catalogue No.CKC122 546.00 1 546.00 1.48 Swing out rotor, 8 × 15ml. For item 1.47 Catalogue No.CKC126: 130.40 1 130.40 1.49 Hand operated centrifuge, capable of 2000rpm with heavy duty buckets, for 15ml conical glass tubes, 4 bucket type. Complete with tubes. Catalogue No.CKC172 136.68 2 273.36 1.50 Centrifuge tubes, 15ml. capacity conical, rimmed, 0.82 20 16.40 glass Catalogue No.CKT014 1.51 “Ohaus” 700 triple beam balance weighing up to 2.61kg with sensitivity of l00mg. Complete with removable polypropylene scoop. 220/240AC. Catalogue No.BJC0041 137.60 2 275.20 1.52 “Salter” EW-3000B balance, weighing up to 3kg with sensitivity up to 1g Mains and portable with rechargeable batteries Catalogue No.BJE286 440.00 2 880.00 1.53 Weather Station to provide data on rainfall, barometric pressure temperature, and humidity. Complete with Stevenson Screen Catalogue No WLM072 and WLM078 146.32 1 146 .32 TOTAL COST OF LOT 1 16234.33 (United States Dollars sixteen thousand two hundred and thirty four and thirty three cents) Recommended Supplier of all above items:- Astell Scientific 172 Brownhill Road LONDON SE6 2DL United Kingdom Justification: Astell Scientific have provided the most comprehensive quotation in terms of price, delivery, and quality, and have vast experience in the equipping of laboratories in the U.K. and Overseas. 3 other companies were contacted. We understand that Astell Scientific are regular suppliers to the FAO. APPENDIX II LOT 2 SPECIFICATIONS UNIT TOTAL COST No COST (USD) (USD) 2.1 Water samplers for taking water at depths up to 30m. Metal construction (preferably bronze) activated by messenger, complete with suitable cable and messengers. 2L capacity 708.80 3 2126.40 Recommended type: “Ruttner” Recommended supplier: Duncan and Associates Beech Court Cart Lane Grange-over-Sands Cumbria LA11 7AF United Kingdom 2.2 Spare messengers for item 2.1 48.00 10 480.00 2.3 Spare cables for item 2.1 60.00 3 180.00 2.4 Sampler for taking bottom sediments of soft or compacted mud. 0.5m tube length. Complete with messenger activated system and 30m cable 7088.00 1 7088.00 Recommended type: “Jenkins” Recommended supplier: As 2.1 2.5 Spare sample tubes for item 2.4 133.00 20 2660.00 2.6 Grab or similar instrument for taking of bottom samples from compacted substrate and small gravelbottoms. Activated by metal messenger and 30m of suitable cable/rope 616.00 1 616.00 Recommended type: “Windermere” Grab (WG1) Recommended supplier: As 2.1 2.7 Bucket bottom sediment sampler, capacity 4.5L for compacted and rocky substrates. Activated by messenger, complete with 30m suitable cable/rope 2100.00 1 2100.00 Recommended type: "Orange Peel" Recommended supplier: As 2.1 2.8 Spare messengers for item 2.8 45.00 10 450.00 2.9 Phytoplankton nets for towing behind survey vessel and workboat, 40cm mouth opening with metal tap end. Meshsize 55 micron 374.00 3 1122.00 Recommended type: Apstein Recommended supplier: As 2.1 2.10 Hand held, sampling nets, wooden handle, metal frame, for obtaining “kick” samples from suitable sites on lakes and river shallows. Mesh size 180 per inch 54.40 5 272.00 Recommended type: FBA Recommended supplier: As 2.1 2.11 Zooplankton nets as 2.9 but mesh size 335 micron 374.00 3 1122.00 2.12 Wooden measuring boards, minimum length size 1.0m, with markings to 0.1cm 92.80 4 371.20 2.13 Weighing bags, large, plastic 35.20 4 140.80 2.14 Metal Secchi Disc. White and black quarters with fixing ring to the upper surface. Minimum 30cm diameter complete with 30m of marked rope, of minimum diameter 7mm. 50.00 3 150.00 2.15 Dispensing reel and rope for use with survey equipment polyethylene reel on coated metal frame contains 60m of pre-stretched nylon/terylene marked off at metre intervals. Complete with side handle. 25.00 6 150.00 2.16 Glass aquarium, 24 × 12 × 18 inches, with metal frame 52.32 5 261.60 Recommended type: Gem Panavision or similar Recommended supplier: C.J. Skilton (Aquarists) Ltd., Great Gibcracks Close Butts Green Sandon CHELMSFORD CM2 7TR Essex United Kingdom 2.17 Sub gravel filters, with air lift and dispersal cap. Size 24 × 12 × 18, inches, plastic base with clear acrylic air lift tube 7.20 5 36.00 2.18 Air pumps to deliver air to item 2.16 22.50 5 112.50 2.19 Air line, air stones, and accessories for item 2.18 21.00 5 105.00 TOTAL COST OF LOT 2 19542.70 (United States Dollars Nineteen thousand five hundred and forty two and seventy cents) APPENDIX II LOT 3 SPECIFICATIONS UNIT No TOTAL COST COST (USD) (USD) 3.1 Binocular microscope, artificial light, phase contrast, moveable binocular head. x8 paired eyepieces, objectives x4, xl0, x20, x40 and xl00 (oil). Quintuple nosepiece, condenser and 3 spare bulbs. Complete with camera attachment and viewing screen 18668.19 1 18668.19 TOTAL COST OF LOT 3 18668.19 (United States Dollars Eighteen thousand six hundred and sixty eight and nineteen cents.) Recommended type: Wild Leitz Diaplan Microscope, with CCTV and photography attachments (see attached quotation) Recommended supplier: Wild Leitz Ltd., 9 435 HEERBURG Switzerland Justification: Technically the finest microscope on the market, for our specific requirements. APPENDIX II LOT 4 SPECIFICATIONS UNIT No. TOTAL COST COST (USD) (USD) 4.1 Outboard engines, 15hp, petrol engines, standard 1600.00 4 6400.00 shaft, manual start, Recommended type Yamaha 15hp Recommended supplier: Yamaha Motor Company P.O. Box 1 IWATA 438 Japan 4.2 Manufacturers recommended spares kit for item 4.1 300.00 4 1200.00 to include spare fuel tank, leads, etc. 4.3 Pairing bar for item 4.1 160.00 2 320.00 4.4 Trial motor cycles, 125cc, 2 stroke liquid cooled, front and rear disc brakes, monocross suspension, 2560.00 2 5120.00 high speed timing retard ignition Recommended type: Yamaha DT125R Recommended supplier: Yamaha Motor Co. Ltd., 2500 Shingai Iwata-Shi Shizuoka-Ken Box 438 Japan 4.5 Manufacturers recommended tool kit for item 4.4. 65.00 2 130.00 TOTAL COST OF LOT 4 13170.00 (United States Dollars

Thirteen thousand one hundred and seventy.)

APPENDIX II LOT 5 SPECIFICATIONS UNIT No. TOTAL COST COST (USD) (USD) 5.1 Electrochemical lake profiler, deep water (up to 30m) portable, type. For electronic measurement of pH,(range 5–10), conductivity (0–25mS/cm,) temperature (0–50°C) oxygen (0–20 mg/1) and light penetration.(400–700nm) all via surface readout. With 30m marked rope. 12400.00 1 12400.00 TOTAL COST OF LOT 5 12400.00 (United States Dollars Twelve thousand four hundred.) Recommended type: Multi-parameter probe system” Recommended supplier: Partech (Electronics) Ltd., Eleven Doors Charlestown St. Austell CORNWALL PL25 3NN United Kingdom Justification.: Sole supplier of this particular item. This model is currently in use in a number of sites, and is rugged and suitable for the weather conditions anticipated at Qinghai Lake. APPENDIX II LOT 6 SPECIFICATIONS UNIT No. TOTAL COST COST (USD) (USD) 6.1 Portable chemical analysis laboratory carrying case, photometer, and instruction book. 622.40 3 1867.20 Recommended type: Palintest PT260 Recommended supplier: Palintest Limited, Palintest House Kingsway Team Valley Gateshead Tyne and Wear NE11 ONS United Kingdom Justification: Price: simplest to use: Supplier is manufacturer:Meets our basic requirements and is not over specified compared with Hach kits 6.2 Modules for item 6.1 Alkalinity (PM188) 10.40 3 31.20 Ammonia (PM152) 15.20 3 45.60 Cooper (PM186 17.60 3 52.80 Iron LR (PM155) 12.80 3 38.40 Manganese (PM173) 12.80 3 38.40 Nitrate (PM163) 18.40 3 55.20 Nitrite (PM109) 32.40 3 97.20 Phosphate LR (PM177) 8.16 3 24.48 Silica (PM181) 16.80 3 50.40 Sulphate (PM154) 5.60 3 16.80 Zinc (PM148) 17.60 3 52.80 6.3 Reagent tablets for use with item 6.1 to carry out

following analyses (1000 each) Alkalinity 0–500mg/l. Code AP188 78.40 1 78.40 Ammonia 0–1 mg/1N. Code AP152, 86.08 1 86.08 Copper 0–5mg/l. Code AP186 117.36 1 117.36 Iron LR 0–1mg/l. Code AP155 125.12 1 125.12 Manganese 0–0.03mg/l.Code AP173 103.04 1 103.04 Nitrate 0–1mg/l. Code AP163 194.80 1 194.80 Nitrite 0–0.5mg/l. Code AP109, 57.60 1 57.60 Phosphate LR 0–4mg/l. Code AP177 134.88 1 134.88 Silica 0–4mg/l. Code AP181 172.00 1 172.00 Sulphate 0–200mg/l. Code AP194 45.44 1 45.44 Zinc 0–4mg/l. Code AP148 194.80 1 194.80 Calcium hardness. Code ATO776 134.40 1 134.40 Chloride. AT0496 163.20 1 163.20 6.4 Environmental multiprobe for monitoring pH (range 4–11), conductivity (500–5000uS/cm), temperature (0–50xC), dissolved oxygen 0–200% saturation, 1167.00 3 3501.00 light penetration, complete with case. Recommended type: WPA Environmental Probes Recommended supplier: Walden Precision Instruments The Old Station Linton Cambridge CB16NW United Kingdom Justification: Proven excellence in the field. Manufacturer is sole supplier of this item. Need for this portable kit to be from a different manufacturer than item 5.1 6.5 Electrofishing system, comprising: Pulsed and 4896.00 4896.00 smooth DC machine 2.5KVA rated generator, petrol powered Anodes, complete with cable Extension

leads for remote fishing Cathode, with 5m cable Extension leads for cathode Handnets, for fish collection Recommended type: WFC6C-10 Recommended supplier: Electracatch 23 Buckley Road Penn Wolverhampton WV4 4LJ United Kingdom Justification: Best U.K. supplier. Most competetive quotation. With a potentially lethal apparatus such as this it is vital that safety is paramount. This machine conforms to relevant international regulations as well as national parameters. UK supplier chosen to ease training in the use of the machine by the Consultant. 6.6 Rubber Chest Waders, for use with item 6.4. Medium size 131.20 3 393.60 6.7 Rubber Aprons, for use with item 6.5 15,00 3 45.00 6.8 Rubber gauntlets, for use with item 6.5 8.00 3 24.00 TOTAL COST OF LOT 6 12333.92 (United States Dollars twelve thousand three hundred and thirty three and ninety two cents) APPENDIX II LOT 7 SPECIFICATIONS UNIT TOTAL COST No. COST (USD) (USD) 7.1 Binoculars, 7 × 50 magnification, with rangefinder and compass, nitrogen filled/ rubber body, adjustable eyepieces, carrying case and strap 410.00 6 2460.00 Recommended type:"Greenkat" 7x50 7.0 with scale and compass Recommended supplier: Gransden Marine Lower Boat House North Pondside Road Chatham Historic Dockyard CHATHAM Kent ME4 4TY Justification: Delivery, and price 7.2 Folding anchors, small, approx 0.5 metre total length incorporating three anchor legs plus attachment ring 24.00 3 72.00 No recommended type Recommended supplier: As 7.1 7.3 Hand held, bearing compass, rubber surround, plastic inserts, dial and pointer 78.40 3 235.20 Recommended type: Offshore Minicompass 2000/S Recommended supplier: As 7.1 7.4 Fire extinguisher for petrol fires dry powder, made to British Standard 5423. 4.5kg capacity 95.00 6 570.00 Recommended Type: Amerex 419 Recommended supplier: As 7.1 7.5 Fire extinguishers for electrical fires. BCF (Halon 1211) made to British Standard 5306. 0.7kg capacity 100.00 6 600.00 Recommended type:Amerex 344 Recommended supplier: As 7.1 7.6 Fire extinguishers for other fires Water, made to British Standard 5423. Capacity 9.5 litres 96.00 6 576.00 Recommended type:Amerex 240 Recommended supplier: As 7.1 7.7 Hand held rechargeable spotlight. 24v or 220/240 250.00 2 500.00 Recommeded type: Perko or similar Recommended supplier: As 7.1 7.8 Fixed spotlight 24v or 220/240. 6" diameter, with tilt and swivel 160.00 2 320.00 Recommended type: Perko 40537 CP 6" Recommended supplier: As 7.1 7.9 Deck floodlights, sealed beam, waterproof, non- corroding, with 25m cable for 24v supply, 50watt. 73.84 4 295.36 Recommended type: Aquasignal 1613400 Recommended supplier: As 7.1 7.10 Fixed ships compass, self compensating, with approximately 20cm. face dial. Conforms to Lifeboat standards. 232.00 1 232.00 Recommended type: Lifeboat Binnacle Compass Recommended supplier: As 7.1 7.11 Life raft. 10 man, with emergency packs and survival equipment, auto-inflation via pull rope 2560.00 1 2560.00 Recommended type: Avon 10 man YM(E) Pack Recommended supplier: As 7.1 7.12 Semi-inflatable lifejackets. Inflation by compressed air pull tag. Complete with mouthpiece for topping up, and whistle. 107.00 6 642.00 Recommended type: “Crewsaver” Crewfit Recommended supplier: As 7.1 7.13 Bouyancy aids, with waist ties. Waistcoat type model, with built in polystyrene blocks. 62.00 10 620.00 Recommended type: Helly Hensen Navigare Recommended supplier:As 7.1 7.14 Power block for lifting nets etc. Maximum weights 250 to 500kgs. 450.56 1 450.56 Recommended type: Strongarm Electric winch Recommended supplier: As 7.1 7.15 Mark/recapture fish tags, for dorsal fin attachment. 25.00 50 1250.00 7.16 “Panjet” dye fish marking system with advised spares 350.00 2 700.00 7.17 Waterproof note books,200x95mm, with 100 pages 17.60 10 176.00 Recommended type: Aquascribe EL1 Recommended supplier: Hawkins and Manwaring Westborough Newark Nottinghamshire NG23 5HJ United Kingdom Justification: Best available 7.18 Waterproof pencils 10.00 10 100.00 Recommended type: Automatic 7mm Recommended supplier: As 7.17 7.19 Pressure storm lanterns. kerosene fuelled, large size 80.00 4 320.00 Recomended type “Hippolito” H/14 Recommended supplier Anglers Den 10 Franklin Road Gillingham Kent ME7 4DF United Kingdom Justification: Used to supplying these items to offshore users. 7.20 Spare mantles for item 7.19 2.00 100 200.00 Recommended supplier: As 7.19 7.21 Spring, dial balance to weigh up to 30kg, x50g, with hook. 115.00 3 345.00 Recommended type: Ruben-Heaton F5110 Recommended supplier: As 7.19 7.22 Spring balance, dial readout, to weigh up to 10kg × l00g with hook. 80.00 3 240.00 Recommended type: Salter Super Samson F4922/22 Recommended supplier: As 7.19 7.23 Spring balance, linear, readout, to weigh up to 5kg. × 50g, with hook. 60.00 3 180.00 Recommended type: Salter Super Samson F4922/11 Recommended supplier: As 7.19 7.24 Spring balance, linear readout, to weigh up to 100g with hook. In 1 gram divisions 67.20 3 201.60 Recommended type: Pesolas No.55 Recommended supplier: As 7.19 7.25 Spring balances, linear readout, to weigh up to 30g with hook. In 0.5g divisions 67.20 3 201.60 Recommended type: Pesolas No.69 Recommended supplier: As 7.19 7.26 Spring balances, linear readout, to weigh up to l0g with hook. In 0.2g divisions 67.20 3 201.60 Recommended type: Pesolas No.52 Recommended supplier: As 7.19 7.27 Battery operated stirrer for vertical mixing and mastication. 50.00 2 100.00 No recommended type or supplier TOTAL COST OF LOT 7 14348.92 (United States Dollars Fourteen thousand three hundred and forty eight and ninety two cents.) APPENDIX II LOT 8 SPECIFICATIONS UNIT No. TOTAL COST COST (USD) (USD) 8.1 Graded mesh sizes from ca 40mm stretched mesh to ca 150mm stretched mesh. Each mesh size makes 1 panel. Each net should be ca 27 meshes deep and 10 to 20m length. individual mesh size panel areas approximately equal.i.e. One panel of 150mm mesh may equal in areas (or3) 40mm nets joined together. 8.2 Experimental Gill Nets (Monofilament) 5 complete fleets of the following Panels 10mm – 30mm. Integral float and lead lines. 8.3 Integral weighted line 500m 8.4 Mounting Twine for mounting net panels on

headline and bottom line. 30 Bobbins (l/2kg) 8.5 Head/Bottom lines (On which net panels are mounted) These will be ca 5mm diameter 2000 metres 8.6 5000 plastic headline floats. 50 large flourescent marker bouys ca 0.5m diameter. 10 large flourescent marker bouys ca lm diameter 8.7 Experimental trawl nets (made up on project) 10 nets each of range of mesh sizes 40 – 60mm 4 nets of ca 10mm mesh. 8.8 Experimental Purse Seines (to be made up on project need FAO tech.advice) Suggest 100m or

150mand 10m deep as easily handled, smallest mesh practicable. 8.9 Warp topes Floating, coloured (?Polypropylene?)

ca 10mm diameter ca 20mm 0 1 coil of each 8.10 Mosquito netting (or fishing net of similar mesh) To be made up on project 50m × 10m (approx) 8.11 Beach seines (To make up on project) Thread nets ca 10mm – 20mm mesh ca 200m × 3m 8.12 Hooks and lines

Selection of graded hooks, nylon snoods, swivels 8.13 Mosquito netting 8.14 Bolting cloth, zooplankton 8.15 Bolting cloth phytoplankton NOTE: No recommendation is made as to suppliers for this particular Lot. It is suggested that the Fishing Industry Group in Building G be consulted as to potential suppliers and possible alternative specification. This should also coincide with a de-briefing of the Technical Advisor, Dr. I.G. Dunn, who is due to return to Rome approximately 30 October 1989. APPENDIX II LOT 9 SPECIFICATION UNIT No TOTAL COST COST (USD) (USD) 9.1 Echo sounder, recording, 24v, multiple depth ranges, for use in saline conditions, for locating fish and comparing with bottom echo 700.00 1 700.00 Recommended type: Eagle Mach 1 or similar Recommended supplier: Eagle Electronics P.O. Box 669 Catoosa Oklahoma 74015 U.S.A Justification: This item should be chosen after discussions with FAO Fishing Industry Division. Recommended type and supplier is guideline. 9.2 Radio telephone system one unit on board the research vessel and one at a fixed land station. Weatherproof casing, digital readout. Able to operate over 120km at high altitudes. 24v. 3200.00 2 6400.00 Recommended type: IC-M700 Recommended supplier: Icom (UK) Ltd., Sea Street Herne Bay Kent CT6 8LD United kingdom Justification: Best available at reasonable price 9.3 Satellite navigation system able to operate on the GPS system. Accurate to 50m or better with route sequencing and course deviation indicator 5977.60 1 5977.60 Recommended type: Raystar 920 GPS Recommended supplier: Raytheon Marine Sales Siljangade 6 DK-2300 Copenhagen Denmark Justification: State of the art technology. Accurate to 15m 9.4 Aluminium work boat, 5m, commercial, semivee hull, built in bouyancy tanks, hand welded seams, hand rail and lockable cabinet for stores 3920.00 2 7840.00 Recommended type: "Sea-Strike" 5m Recommended supplier: Latent Bondings Limited Adini House Wolverhampton Road Wedges Mills Cannock Staffordshire WS11 1ST United Kingdom Justification: Best available. One of few available with welded seams. TOTAL COST OF LOT 9 20917.60 (United States Dollars Twenty thousand nine hundred and seventeen and sixty cents) APPENDIX II LOT 10 LITERATURE UNIT No TOTAL COST COST (USD) (USD) 10.1 Plankton and Fisheries Author: J. Grahame Publisher: Ed. Arnold ISB.No. 0.7131.2941.7 19.12 1 19.12 10.2 Introduction to Practical Fisheries Science Author: Will Royce Publisher: Academic Press ISB No. 0.12.600960.0 48.00 1 48.00 10.3 Biology of Fish Growth Author: A.H.Weatherly & H.S.Gill Publisher: Academic Press ISB No. 0.12.739055.3 64.00 1 64.00 10.4 The Control of Fish Migration Author: R.J.F. Smith Publisher: Academic Press ISB No. 3.540.13707.6 70.16 1 70.16 10.5 Control of Fish Quality Author: Connell Publisher: Fishing News Books ISB No. 0.85238.105.0 20.00 1 20.00 10.6 Fishing and Stock Fluctuation Author: Laevastu & Favorite Publisher: Fishing News Books ISB No. 0.85238.155.7 40.00 1 40.00 10.7 A Treatise on Limnology Volumes 1, 2, & 3. Author: Hutchinson Publisher: Wiley 280.00 1 280.00 10.8 Secondary Production in Fresh Water IBP Hand Book 17 Author: Downing Rigler Publisher: Blackwell ISB No. 0.632.00616.1 46.40 1 46.40 10.9 Phytoplankton Ecology Author: Harris Publisher: Chapman & Hall ISB No. 0.412.24330.X 60.80 1 60.80 10.10 Fishing with Electricity Editor: R.Vibert Publisher: F.A.O. ISB No. 0.85238.047 16.00 1 16.00 10.11 How to Make a Set of Nets 14.00 1 14.00 Author: John Garner Publisher: Fishing News Books ISB No. 0.85238.031.3 10.12 Par Trawling & Pair Author: D. Thomson Publisher: Fishing News Books ISB No. 0.85238.087.9 28.80 1 28.80 10.13 The Study of Benthic Communities Author: R.Parker Publisher: Elsevier ISB No. 0.444.41203.4 36.80 1 36.80 10.14 A Manual of Chemical & Biological Methods for Sea Water Analysis Author: Parsons, Malta, Malli Publisher: Pergamon Press ISB No. 0.08.030287.4 11.20 1 11.20 10.15 Handbook of Limnology Author: J. Schwoerbel Publisher: Ellis Horwood ISB No. 0.7458.0033.5 49.60 1 49.60 10.16 The Ecology of Algae Author: Round ISB No. 0.521.26906.7 Publisher: Cambridge University Press 52.00 1 52.00 10.17 Introduction to Fresh Water Algae Author: A. Pentecost Publisher: Richmond Publication ISB No. 0.85546143.8 15.92 1 15.92 10.18 Invertebrates of Streams and Rivers A Key to Identification Author: M. Quigley Publisher: Edward Arnold ISB No. 0.7131.0091.5 9.52 1 9.52 10.19 Animal-Life in Fresh Water Author: Melllanby Publisher: Chapman and Hall ISB No. 0.412.21360.5 11.12 1 11.12 10.20 Commercial Fishing Methods Author: John Sainsbury Publisher: Fishing News Books ISB No. 0 85238 142 5 17.60 1 17.60 10.21 Fisheries Sonar Author: R.B. Mitson Publisher: Fishing News Books ISB No. 0 85238 124 7 48.00 1 48.00 10.22 Netting Materials for Fishing Gear Author: G. Klust Publisher: FAO ISB No. 0 85238 118 2 18.80 1 18.80 10.23 Finding Fish with Echo-sounders Author: J.Burczynski and M. Ben Yami Publisher: FAO Training Series No.7 ISB No. 92 5 101327 6 9.00 1 9.00 10.24 Stocking and Introduction of Fish in lakes and reservoirs in the ASEAN countries Author: FAO Publisher: FAO Fisheries Tech. Paper No. 236 ISB No. 92 5 101356 7 6.00 1 6.00 10.25 The Potential yield of Fish Stocks Author: FAO Publisher: FAO Fisheries Tech. Paper No.242 ISB No. 92 5 101492 2 6.00 1 6.00 TOTAL COST OF UNIT 10 998.84 (United States Dollars Nine hundred and ninety eight and eighty four cents) NOTE: This list represents the basis of the establishment of a relevant library and should be updated by all members of the Project Team at an early opportunity. APPENDIX II LOT 11 SPECIFICATIONS CHEMICALS REQUIRED Acetone 5L Aluminium Sulphate 500g Ammonium molybdate 500g Anti-bumping granules 100g Ascorbic acid 100g

Barium Chloride 500g Boric acid 500g Brilliant Yellow 50g

Calcium carbonate 1000g Calcium sulphate 500g

Devardos Alloy 100g

Ethanol 5L

Ferrous sulphate 500g Ferrous ammonium sulphate 500g Formaldehyde solution 25L

Hexanol 500ml Hydrochloric acid 10L

Manganous sulphate 1000g Methyl Red 25g Methylene Blue 50g Magnesium oxide 100g Mercuric sulphate 100g Mercuric nitrate 100g Methyl orange 25g Magnesium carbonate 500g Methanol 5L Methyl cellulose 100g Magnesium coil 100g

Nitric acid 10L

Phenophthalein 25g Potassium dihydrogen phosphate 500g Potassium dichromate 1000g Potassium chromate 500g Potassium aluminium sulphate 100g Potassium iodate 100g Potassium iodide 100g Propanol-iso 5L

Sodium iodide 1000g Sodium thiosulphate 1000g Starch 500g Sulphuric acid 10L Sodium hydroxide 1000g Sodium antimony tartrate 100g Silver sulphate 100g Silver nitrate 100g Sodium chloride 1000g Sodium bicarbonate 1000g Solochrome black 25g Sodium sulphite 500g Sodium dihydrogen EDTA 1000g

Toluene 5L Triethanolamine 500ml NOTE: These chemicals are recommended as the minimum requirement to enable the Institute of Aquatic Products to carry out the following analyses: BOD, Ammoniacal Nitrogen, Nitrate Nitrogen, phosphate, COD, Chlorophyll'A', Magnesium, Calcium, Sodium, Potassium, Barium, Silica, Chloride, Alkalinity, Hardness and Sulphate. The list is prepared assuming the use of 'Standard Methods' of analysis, and that equipment and chemicals already existing in the Institute are utilised, together with those supplied from overseas. The laboratory should be encouraged to 'customise' its analytical procedures as soon as possible. The above list should enable a considerable number of tests to be carried out before replenishment is required. These analytical procedures should be used to back up and confirm field investigations carried out on portable analysis kits, to undertake special and detailed analyses on plant and animal material as required, and to carry out those tests not suitable for field analysis. It is recommended that as many of these items as possible are purchased in China, or from a Far Eastern agency of a European Company. All chemicals should be of "Analytical Grade".