FI:DP/CPR/88/077 Field Document 9 December 1990 PEOPLE'S REPUBLIC OF CHINA EQUIPMENT REQUIREMENTS Report prepared for the project Fisheries development in Qinghai Province People's Republic of China by C.J. Smith Consultant FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS Rome, 1990 TABLE OF CONTENTS Page 1 INTRODUCTION AND TERMS OF REFERENCE 1 2 CURRENT STATUS OF EQUIPMENT 1 3 CURRENT STATUS OF PERSONNEL 1 Appendix I EQUIPMENT CURRENTLY AT THE INSTITUTE OF AQUATIC 3 PRODUCTS Appendix II: SPECIFICATIONS - LOTS 1-11 4 1. INTRODUCTION AND TERMS OF REFERENCE The project aims to assist development of fish production in Qinghai Province in two ways: by producing a management plan for the important naked carp fishery on Qinghai Lake and by demonstration of the technology of rainbow trout culture. This report identifies the equipment and monitoring requirements of the project by the assessment of: – the current equipment status of the laboratory of the Institute of Aquatic Products, Xining City, Qinghai Province, People's Republic of China – the equipment requirements of the project to enable a basic limnological investigtion to be undertaken in line with the objectives of the project. As part of the project operations, FAO assigned Mr C.J. Smith as consultant from 6 September to 25 October 1989. After consul-tations with project Chief Technical Adviser, Mr David Edwards and with FAO HQ staff in Rome the consultant's terms of reference were modified to take account of the above requirements. 2. CURRENT STATUS OF EQUIPMENT The laboratory at the Institute of Aquatic Products is in a dilapidated condition; however, there are number of old but functional pieces of equipment (listed in Appendix ]). Apart from a small portable pHmeter, all equipment appears fixed. 3. CURRENT STATUS OF PERSONNEL After discussion with the present staff of the Institute and observations on the status of the laboratory and the current work programme, it is evident that, although the level of training appears to be adequate, there is a considerable lack of practical experience both in the field and in the laboratory. It is assumed that this could be rectified by a relatively short training programme before the full project field work commences. Detailed discussions with the CTA and HQ staff in Rome have stressed the need to undertake limnological investigations only inasfar as they affect the fishery. It is not the purpose of this project to conduct a detailed academic survey of the environment and biota of Qinghai Lake. Consequently, numerous detailed precise analyses of a large variety of parameters were not necessary. It is essential, however, that in keeping the institution-building aspects of this project, a complete, operational laboratory is equipped with the necessary analytical and investigative equipment. To this end, the equipment requirements have been kept to a minimum and emphasis placed on any physical and chemical analyses being carried out, where possible, in the field, with the fixed laboratory facility in Xining City being used for those parameters not easily tested in the field (e.g., BOD, COD, protein, etc.) and for occasional confirmatory analyses of the performance of the field kits. The equipment required is listed in Appendix II, and is divided into a series of lots for easy categorization and procurement. Assessment has been made of some of the available models on the market, and where appropriate, recommendations made as to the best available option. This decision has been based on delivery, cost, technical specia1ications, previous "hands-on" experience of the consultant, and first-hand knowledge of the project site and capabilities of local staff. It is imperative that all the equipment noted is ordered and delivered without delay in order that the project is ready to commence in March 1990 with the personnel of the Institute trained in its use. It is understood that throughout the project's 4-year span, provision must be made for the replacement of consumable items and the purchase of technical literature as it is identified. The costings shown are indicate and precise costings must await pro-forma invoices from suppliers, and will be subject to currency exchange rate fluctuations. For the purposes of this report a conversion rate of £1 = US$ 1.60 is applied. Given that the limnological investigations should pertain to fisheries aspects only, the monitoring programme needs to be very flexible once the survey vessel starts work in spring 1990. The provision of a depth profiler to carry out determinations of pH, temperature, conductivity, dissolved oxygen and light intensity at depth, will considerably reduce the number of analyses needed to establish a baseline reference for the identification of water masses. Once this baseline is established, areas of the lake important in terms of the fishery can be examined in more detail. In order that remote upstream spawning sites can be visited and examined, and the fact that the difficult terrain in the area precludes visits by four-wheel vehicles overland, or by the workboats upstream, a recommendation is made f or the purchase of 2 off-road motorcycles. APPENDIX I Equipment currently at the Institute of Aquatic Products, Xining City, Qinghai Province, People's Republic of China NON-EXPENDABLES Electronic, double pan balance and weights 1 Manual, double pan balance, with weights 1 Electric, distilled water still, 10L/hour 1 20L/hour 1 Electric Oven, temperature adjustable (Note 1) 50–250°C 1 Electric muffle furnace (Note 2) range 0–950°C 1 Spectrophotometer, range 360nm–800nm, plus cuvettes.(Note 3). l Spectrophotometer, range 420nm–700nm, plus cuvettes.(Note 3). l pH meter, portable 1 pH and redox potential meter, fixed, 1 Centrifuge, cup capacity 250 ml 1 Dessicators, glass, non-vacuum 3 Water bath, electric, four rings, rectangular 1 Beam balance, maximum load lOOOg 1 EXPENDABLES (Note 4) Beakers, mainly 500ml capacity 25 Flasks, Erlenmeyer, 500,250 and 100 ml capacity 30 Flasks, volumetric, 500,250 and 100 ml capacity 30 Pipettes, bulb and straight type, all capacities 50 Burettes, ‘B’ grade accuracy, 50ml capacity 15 Watch glasses, various sizes 50 Bottles, dropping,100, 50 and 25ml capacity 10 Bottles, sample, 500ml capacity 5 Bottles, wash, operated by mouth, 1L capacity 2 Bottles, reagent, various sizes, plastic stoppers 20 Bottles, BOD, ground glass stoppers 2 Condensers, coil type, 2 Test tubes, with stoppers, 50ml capacity 30 Filter funnels, mainly small sizes 5 Measuring cylinders, 100, 250, and 500ml capacity 20 NOTES: 1. Thermostat broken. To be purchased locally. 2. Not connected, but assured is in working order. 3. Old model, but apparently in working order. 4. Many pieces of glassware contain chemicals or solutions that may render washing and re-use impractical. APPENDIX II LOT 1 LABORATORY CONSUMABLES LOT 2 SURVEY EQUIPMENT LOT 3 RESEARCH MICROSCOPE LOT 4 TRANSPORT LOT 5 LAKE CHEMISTRY PROFILER LOT 6 DATA COLLECTION APPARATUS LOT 7 SURVEY VESSEL REQUISITES LOT 8 FISHING NETS LOT 9 NAVIGATION AND COMMUNICATION LOT 10 LITERATURE LOT 11 CHEMICALS APPENDIX II LOT 1 SPECIFICATIONS UNIT No. TOTAL COST COST (USD) (USD) 1.1. Pressure tubing, red, rubber 5mm bore, 13mm outside diameter, 10m coil, 4mm thick wall. Catalogue No.TMG134 17.60 1 17.60 1.2 Red rubber tubing, general laboratory purpose grade , 5mm bore, 8mm outside diameter, 1.5mm wall, 10m coil. Catalogue No.TMG116 7.36 1 7.36 1.3 Sample bottles, square, wide mouth with push in leakproof seals and easy to grip caps. Caps must have a toggle for attachment of label. HDPE or similar material 2L capacity Catalogue No.BGE242 3.81 500 1905.00 1.4 As 1.3 but 1L capacity Catalogue No.BGE240 2.64 100 264.00 1.5 As 1.3 but 500ml capacity Catalogue No.BGE236 2.09 100 209.00 1.6 As 1.3 but 50ml capacity and LDPE material Catalogue No.BGE218 0.81 50 40.50 1.7 Round, wide mouth, HDPE, robust, aspirators. Fitted with minimum 12mm bore stopcock welded to the bottle. Minimum of two carrying handles. 50L capacity. Catalogue No.BGH070 94.08 2 188.16 1.8 Round, sloping shoulder, LDPE, distilled water wash bottles. Minimum capacity 250ml with screw cap leakproof fitting and swivel delivery tubes Catalogue No.WGF530 1.55 8 12.40 1.9 Thermometers, total immersion, 300mm long, glass, yellow back glass, mercury filled with reinforced bulb. -10°C to 1°C in 1°C gradation. Catalogue No. TNG040 2.40 10 24.00 1.10 Glass microscope slide, sandblasted edges, 1.0 to 1.2mm nomimal thickness, 76x26mm, plain. “Blue Star” or similar type. Catalogue No.MLP024 0.0258 500 12.90 1.11 Cover slips, glass, for item 1.11, 22mm square, thickness, 0.155mm – 0.185mm Catalogue No.MLP058 0.024 1000 24.00 1.12 Metal bottle store to meet Highly Inflammable Liquids Regulations. Capacity 8 × 2.5L Winchester type bottles, with partitioning trays and two carrying handles. Catalogue No.BMP176 175.20 3 525.60 1.13 Beakers, borosilicate glass, with spout and graduations, low form, Duran type, 50ml capacity Catalogue No.BKZOOO 2.18 50 109.00 1.14 As 1.13 but 100ml capacity Catalogue No.BKZ002 2.10 50 105.00 1.15 As 1.13 but 250ml capacity Catalogue No.BKZOO6 2.29 50 114.50 1.16 As 1.13 but 1000ml capacity Catalogue No.BKZO16 5.39 10 53.90 1.17 Kjeldahl apparatus comprising, electothermal 220/240AC heating mantle with six heating recesses to take 100 -300ml capacity Kjeldahl flask apparatus total wattage 1800v maximum. 6 complete Kjeldahl “Quickfit” assemblies comprising condenser, dropping funnel, Kjeldahl flask, multiple adapter, spring clips, delivery adapter and splash head.