Comprehensive Analysis of Varicella-Zoster Virus Proteins Using a New Monoclonal Antibody Collection
Comprehensive Analysis of Varicella-Zoster Virus Proteins Using a New Monoclonal Antibody Collection Tihana Lenac Roviš,a Susanne M. Bailer,b,c Venkata R. Pothineni,b Werner J. D. Ouwendijk,d Hrvoje Šimic´,a Marina Babic´,e Karmela Miklic´,a Suzana Malic´,a Marieke C. Verweij,f Armin Baiker,b,g Orland Gonzalez,h Albrecht von Brunn,b Ralf Zimmer,h Klaus Früh,f Georges M. G. M. Verjans,d Stipan Jonjic´,a,e Jürgen Haasb,i Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatiaa; Max von Pettenkofer Institut, Ludwig-Maximilians Universität München, München, Germanyb; University of Stuttgart, Biological Interfacial Engineering, Stuttgart, Germanyc; Department of Viroscience, Erasmus Medical Centre, Rotterdam, the Netherlandsd; Department of Histology and Embryology, Faculty of Medicine, University of Rijeka, Rijeka, Croatiae; Vaccine and Gene Therapy Institute, Oregon Health and Science University, Beaverton, Oregon, USAf; Bavarian Health and Food Safety Authority, Oberschleissheim, Germanyg; Institute for Bioinformatics, Ludwig-Maximilians Universität München, München, Germanyh; Division of Pathway Medicine, University of Edinburgh, Edinburgh, United Kingdomi Varicella-zoster virus (VZV) is the etiological agent of chickenpox and shingles. Due to the virus’s restricted host and cell type tropism and the lack of tools for VZV proteomics, it is one of the least-characterized human herpesviruses. We generated 251 monoclonal antibodies (MAbs) against 59 of the 71 (83%) currently known unique VZV proteins to characterize VZV protein expression in vitro and in situ. Using this new set of MAbs, 44 viral proteins were detected by Western blotting (WB) and indi- rect immunofluorescence (IF); 13 were detected by WB only, and 2 were detected by IF only.
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